pycom16g25370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0016858
Physical Location & Seq
Reverse (-)
27214980 .. 27218771
3792 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g25370.2

Sequence Viewer

Length: 1644 bp
ATGGCCGAACCACCGAGTAGTCCTGAAGTTGAAGCTAGTACTAGTACTGTGCAGCAAGAGTTGATCAGATCAGCAGCGGTCGAAATTGCGTATTTAGCAGGTGATAATATTACCACTCCAGATGTGCATGGGGCGGGCATGAAAATTGAGAGGGCAAGGGATGCATACAAAGTATATGCAGGGTTTGAAGAGAAGCCTAACAAGGTGGAGATTTTGAGTTGGTATTTTTATGAGTTGTGCTCCCACTTCATTCATCTTGTTCTTATCCCGATCGTCTTCCCTCTCATCATCACCCAAATCGGTGACGCTGCACGCGACTCTGGGTCTGGGGAGGGGTTAGCCCGCAAGTACAAGGGCGTGGCCGCTTGCACTCACAAGGAAACCATACTGTACCTTAGACTCACAAACAGGTCATTACTCTCCAAATACTCACCTCTAGAGTGGACTTCAATTTCATGGGGTATAGGTCTAGCTCTAGCAGCTCCACTCCTTCACTTTGTCTCCATCATCCTTGACCATGGCTACAAGCAACCCCTCGTTGCCGGCGCCGCAGCCGCCATCGGGTCCATCTTCTGTCTTCCAGCTGGCTTTTTCCGAAACATCTGGCTCTTTCCACCGTACATTGCCGCCATAGTTTGTGCCAACGTCATTGCCACCGCCTGCCACACCCGCCACCTCGGCCTATTGGTCCGCGGGTTCACGGGACCAACTATCCGAAAATCCCAATTCCAAGCCCGAAGAGCAGTCTCCGCCTGGCTTTCGCTCTACGCAGCCGCGGCGGGCTCATTAGGCTCTGCCGTCTTCTCATCCTTCGCTTACCACATGCTTAGGGAAGATGAAGAGTTGCTAGGGCTTTGGATTGTGTCAATCTTTGGTGGGATCATATGGTTCACTGGGATATTCCATTTCTTTGTGACCAATAGGCCCTGTGTGAATCCACCCATCTCAAAGTCCCATGCACTTTCAGTCTTCAAGTTTCCTCATGCAGTTGGAGGACTTGTGGGAACTTTCCTCTCCTCGTTCACATCAATGTGTATTTTCACAGGGGGAGTGCTTTACCTAGTTGGGCAGCTTTGCTATGATCCAAAAGCATTGCTATTTTTTTTCCTAACTTACTTCATCTTCCCCTTGATTTCTCTCCCAATGTTACAACCACTGCAGCATCTCATCAAGGCGGACGCCATCAAAATGCAACTCATGGGTTTCTTCCTATCAGCACTCACTTCCGGGTCCGGATTCTATTTCCGGAACTCACTTTGGGGGCATCCTCATGTCCTCTTCTTCGGTGCGGTTCAAAGCACGTCCGCGGGGCTTTTGCACGCCTTTGGACGGGTTCTGCTGATGGATTGTTCGCCGCCAGGGAAAGAAGGCGCTTTCTCCACATGGCATTCCTGGATCAAAGCTCTTGGCACATGTGTTGGCTTCGCATTGGCAACGGCAGTCCCAGGAAACCTGTTCACTTCTTTCGGGCTGTCATTTTTAACCACTGTGTGTGGAGTGGTGATTCTGATTTTTGGGAACGTCAGCGATTATGGAGGAGCCAAAGCTGCCGGCCATGTGAGGCACGACAGCGAGCGAGGAGGCTCGCCTGTTAGTGGCCTTGAGGCTGCCTATGAGACACAAGAACCAGCAGCTGTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

548

Amino Acids

59.25

Weight (kDa)

8.4

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016858)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g23600
malus_domestica MD00G1000300.v1.1
prunus_persica Prupe.5G024200_v2.0.a1
pyrus_communis pycom16g25370
rosa_chinensis RchiOBHm_Chr7g0212901
rosa_laevigata RLG00000002857
rosa_multiflora Rmu_ssc0000491.1_g000003
rosa_roxburghii Rroxscaffold_3G00246440
rosa_rugosa Rorug07G0137500
rosa_samantha Rh7AG269100 Rh7BG264700 Rh7CG288300 Rh7DG277300
rosa_wichuraiana Rw7G023380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 545
AccII CGCG 4 cut(s) 315, 693, 776, 1307
AccIII TCCGGA 2 cut(s) 1232, 1245
AclWI GGATC 3 cut(s) 887, 1076, 1403
AcoI YGGCCR 3 cut(s) 3, 360, 1552
AcuI CTGAAG 1 cut(s) 45
AcyI GRCGYC 2 cut(s) 546, 1179
AdeI CACNNNGTG 1 cut(s) 1491
AfaI GTAC 6 cut(s) 40, 46, 350, 392, 620, 1638
AfiI CCNNNNNNNGG 3 cut(s) 561, 1329, 1595
AflIII ACRYGT 1 cut(s) 1412
AgsI TTSAA 5 cut(s) 32, 188, 450, 973, 1295
AhdI GACNNNNNGTC 1 cut(s) 322
AhlI ACTAGT 1 cut(s) 41
AjiI CACGTC 1 cut(s) 1302
AjnI CCWGG 4 cut(s) 752, 1357, 1391, 1444
AluBI AGCT 8 cut(s) 35, 473, 482, 584, 1072, 1403, 1547, 1634
AluI AGCT 8 cut(s) 35, 473, 482, 584, 1072, 1403, 1547, 1634
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 3 cut(s) 505, 751, 1610
AlwI GGATC 3 cut(s) 887, 1076, 1403
AlwNI CAGNNNCTG 1 cut(s) 1634
Aor13HI TCCGGA 2 cut(s) 1232, 1245
AoxI GGCC 6 cut(s) 3, 360, 679, 923, 1552, 1597
AspLEI GCGC 2 cut(s) 548, 1373
AspS9I GGNCC 5 cut(s) 564, 688, 704, 924, 1230
AsuC2I CCSGG 1 cut(s) 1228
AsuHPI GGTGA 5 cut(s) 113, 283, 314, 423, 1513
AvaII GGWCC 4 cut(s) 564, 688, 704, 1230
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 1 cut(s) 785
BbsI GAAGAC 4 cut(s) 268, 569, 793, 961
Bbv12I GWGCWC 1 cut(s) 242
BccI CCATC 6 cut(s) 512, 566, 575, 950, 1190, 1336
BceAI ACGGC 2 cut(s) 782, 1452
BciT130I CCWGG 4 cut(s) 754, 1359, 1393, 1446
BclI TGATCA 1 cut(s) 63
BcnI CCSGG 1 cut(s) 1228
BcoDI GTCTC 3 cut(s) 505, 751, 1610
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 7 cut(s) 36, 42, 437, 470, 476, 848, 1061
BfmI CTRYAG 1 cut(s) 1157
BfoI RGCGCY 2 cut(s) 549, 1374
BfuAI ACCTGC 1 cut(s) 89
BglI GCCNNNNNGGC 1 cut(s) 678
BmcAI AGTACT 2 cut(s) 40, 46
Bme1390I CCNGG 5 cut(s) 754, 1228, 1359, 1393, 1446
Bme18I GGWCC 4 cut(s) 564, 688, 704, 1230
BmeRI GACNNNNNGTC 1 cut(s) 322
BmgBI CACGTC 1 cut(s) 1302
BmgT120I GGNCC 5 cut(s) 564, 688, 704, 924, 1230
BmiI GGNNCC 5 cut(s) 547, 565, 705, 1231, 1540
BmrFI CCNGG 5 cut(s) 754, 1228, 1359, 1393, 1446
BmrI ACTGGG 1 cut(s) 903
BmsI GCATC 3 cut(s) 151, 1171, 1273
BmuI ACTGGG 1 cut(s) 903
BpiI GAAGAC 4 cut(s) 268, 569, 793, 961
BplI GAGNNNNNCTC 2 cut(s) 224, 256
BpmI CTGGAG 1 cut(s) 102
Bpu10I CCTNAGC 1 cut(s) 827
BpuEI CTTGAG 1 cut(s) 1622
BpuMI CCSGG 1 cut(s) 1228
BsaHI GRCGYC 2 cut(s) 546, 1179
BsaJI CCNNGG 7 cut(s) 517, 676, 691, 774, 1305, 1358, 1444
BsaWI WCCGGW 2 cut(s) 1232, 1245
BsaXI ACNNNNNCTCC 4 cut(s) 485, 515, 984, 1014
Bsc4I CCNNNNNNNGG 3 cut(s) 561, 1329, 1595
Bse118I RCCGGY 2 cut(s) 542, 1550
Bse1I ACTGG 1 cut(s) 898
Bse3DI GCAATG 3 cut(s) 621, 648, 1091
BseAI TCCGGA 2 cut(s) 1232, 1245
BseBI CCWGG 4 cut(s) 754, 1359, 1393, 1446
BseDI CCNNGG 7 cut(s) 517, 676, 691, 774, 1305, 1358, 1444
BseGI GGATG 4 cut(s) 166, 507, 806, 1264
BseLI CCNNNNNNNGG 3 cut(s) 561, 1329, 1595
BseMI GCAATG 3 cut(s) 621, 648, 1091
BseNI ACTGG 1 cut(s) 898
BseRI GAGGAG 3 cut(s) 1006, 1551, 1593
BsgI GTGCAG 2 cut(s) 71, 294
Bsh1236I CGCG 4 cut(s) 315, 693, 776, 1307
Bsh1285I CGRYCG 2 cut(s) 81, 273
BshFI GGCC 6 cut(s) 5, 362, 681, 925, 1554, 1599
BshNI GGYRCC 1 cut(s) 545
BsiEI CGRYCG 2 cut(s) 81, 273
BsiHKAI GWGCWC 1 cut(s) 242
BsiSI CCGG 5 cut(s) 543, 1227, 1233, 1246, 1551
BslFI GGGAC 3 cut(s) 717, 937, 1427
BslI CCNNNNNNNGG 3 cut(s) 561, 1329, 1595
BsmAI GTCTC 3 cut(s) 505, 751, 1610
BsmFI GGGAC 3 cut(s) 717, 937, 1427
BsmI GAATGC 1 cut(s) 1387
BsnI GGCC 6 cut(s) 5, 362, 681, 925, 1554, 1599
Bsp1286I GDGCHC 2 cut(s) 242, 785
Bsp13I TCCGGA 2 cut(s) 1232, 1245
Bsp1407I TGTACA 1 cut(s) 1636
Bsp143I GATC 6 cut(s) 63, 68, 270, 879, 1081, 1395
Bsp19I CCATGG 1 cut(s) 517
BspANI GGCC 6 cut(s) 5, 362, 681, 925, 1554, 1599
BspEI TCCGGA 2 cut(s) 1232, 1245
BspFNI CGCG 4 cut(s) 315, 693, 776, 1307
BspLI GGNNCC 5 cut(s) 547, 565, 705, 1231, 1540
BspMAI CTGCAG 1 cut(s) 1161
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 3 cut(s) 887, 1076, 1403
BspQI GCTCTTC 1 cut(s) 733
BspT107I GGYRCC 1 cut(s) 545
BsrDI GCAATG 3 cut(s) 621, 648, 1091
BsrFI RCCGGY 2 cut(s) 542, 1550
BsrGI TGTACA 1 cut(s) 1636
BsrI ACTGG 1 cut(s) 898
BssAI RCCGGY 2 cut(s) 542, 1550
BssECI CCNNGG 7 cut(s) 517, 676, 691, 774, 1305, 1358, 1444
BssMI GATC 6 cut(s) 63, 68, 270, 879, 1081, 1395
BssNI GRCGYC 2 cut(s) 546, 1179
BssT1I CCWWGG 1 cut(s) 517
Bst2UI CCWGG 4 cut(s) 754, 1359, 1393, 1446
Bst4CI ACNGT 4 cut(s) 49, 390, 618, 1489
Bst6I CTCTTC 4 cut(s) 183, 733, 834, 1283
BstACI GRCGYC 2 cut(s) 546, 1179
BstAPI GCANNNNNTGC 1 cut(s) 161
BstAUI TGTACA 1 cut(s) 1636
BstDEI CTNAG 2 cut(s) 395, 827
BstDSI CCRYGG 4 cut(s) 517, 691, 774, 1305
BstF5I GGATG 4 cut(s) 166, 507, 806, 1264
BstFNI CGCG 4 cut(s) 315, 693, 776, 1307
BstH2I RGCGCY 2 cut(s) 549, 1374
BstHHI GCGC 2 cut(s) 548, 1373
BstKTI GATC 6 cut(s) 66, 71, 273, 882, 1084, 1398
BstMAI GTCTC 3 cut(s) 505, 751, 1610
BstMBI GATC 6 cut(s) 63, 68, 270, 879, 1081, 1395
BstMCI CGRYCG 2 cut(s) 81, 273
BstNI CCWGG 4 cut(s) 754, 1359, 1393, 1446
BstNSI RCATGY 2 cut(s) 826, 1416
BstSCI CCNGG 5 cut(s) 752, 1226, 1357, 1391, 1444
BstSFI CTRYAG 1 cut(s) 1157
BstUI CGCG 4 cut(s) 315, 693, 776, 1307
BstV2I GAAGAC 4 cut(s) 268, 569, 793, 961
BsuRI GGCC 6 cut(s) 5, 362, 681, 925, 1554, 1599
BtgI CCRYGG 4 cut(s) 517, 691, 774, 1305
BtrI CACGTC 1 cut(s) 1302
BtsCI GGATG 4 cut(s) 166, 507, 806, 1264
BtsI GCAGTG 1 cut(s) 1154
BtsIMutI CAGTG 3 cut(s) 891, 1154, 1485
BveI ACCTGC 1 cut(s) 89
CaiI CAGNNNCTG 1 cut(s) 1634
CfoI GCGC 2 cut(s) 548, 1373
Cfr10I RCCGGY 2 cut(s) 542, 1550
Cfr13I GGNCC 5 cut(s) 564, 688, 704, 924, 1230
Cfr42I CCGCGG 3 cut(s) 694, 777, 1308
CseI GACGC 2 cut(s) 314, 1187
Csp6I GTAC 6 cut(s) 39, 45, 349, 391, 619, 1637
CviQI GTAC 6 cut(s) 39, 45, 349, 391, 619, 1637
DdeI CTNAG 2 cut(s) 395, 827
DinI GGCGCC 1 cut(s) 547
DpnI GATC 6 cut(s) 65, 70, 272, 881, 1083, 1397
DpnII GATC 6 cut(s) 63, 68, 270, 879, 1081, 1395
DraIII CACNNNGTG 1 cut(s) 1491
DriI GACNNNNNGTC 1 cut(s) 322
EaeI YGGCCR 3 cut(s) 3, 360, 1552
Eam1104I CTCTTC 4 cut(s) 183, 733, 834, 1283
Eam1105I GACNNNNNGTC 1 cut(s) 322
EarI CTCTTC 4 cut(s) 183, 733, 834, 1283
EciI GGCGGA 2 cut(s) 739, 1190
Eco130I CCWWGG 1 cut(s) 517
Eco24I GRGCYC 1 cut(s) 785
Eco47I GGWCC 4 cut(s) 564, 688, 704, 1230
Eco57I CTGAAG 1 cut(s) 45
EcoO109I RGGNCCY 1 cut(s) 924
EcoRII CCWGG 4 cut(s) 752, 1357, 1391, 1444
EcoT14I CCWWGG 1 cut(s) 517
EcoT22I ATGCAT 1 cut(s) 166
EcoT38I GRGCYC 1 cut(s) 785
EgeI GGCGCC 1 cut(s) 547
EheI GGCGCC 1 cut(s) 547
ErhI CCWWGG 1 cut(s) 517
FaqI GGGAC 3 cut(s) 717, 937, 1427
FauI CCCGC 6 cut(s) 127, 350, 677, 686, 772, 1300
FauNDI CATATG 1 cut(s) 884
FbaI TGATCA 1 cut(s) 63
FokI GGATG 4 cut(s) 173, 494, 793, 1251
FriOI GRGCYC 1 cut(s) 785
FspBI CTAG 7 cut(s) 36, 42, 437, 470, 476, 848, 1061
GlaI GCGC 2 cut(s) 547, 1372
GsuI CTGGAG 1 cut(s) 102
HaeII RGCGCY 2 cut(s) 549, 1374
HaeIII GGCC 6 cut(s) 5, 362, 681, 925, 1554, 1599
HapII CCGG 5 cut(s) 543, 1227, 1233, 1246, 1551
HgaI GACGC 2 cut(s) 314, 1187
HhaI GCGC 2 cut(s) 548, 1373
Hin1I GRCGYC 2 cut(s) 546, 1179
Hin6I GCGC 2 cut(s) 546, 1371
HinP1I GCGC 2 cut(s) 546, 1371
HinfI GANTC 5 cut(s) 317, 399, 934, 1236, 1504
HpaII CCGG 5 cut(s) 543, 1227, 1233, 1246, 1551
HphI GGTGA 5 cut(s) 113, 283, 314, 423, 1513
Hpy166II GTNNAC 6 cut(s) 444, 699, 891, 1023, 1458, 1639
Hpy188I TCNGA 4 cut(s) 68, 596, 716, 1509
Hpy188III TCNNGA 6 cut(s) 23, 119, 268, 437, 1233, 1246
Hpy8I GTNNAC 6 cut(s) 444, 699, 891, 1023, 1458, 1639
HpyAV CCTTC 3 cut(s) 500, 820, 1361
HpyCH4III ACNGT 4 cut(s) 49, 390, 618, 1489
HpyCH4IV ACGT 3 cut(s) 645, 1301, 1521
HpyF3I CTNAG 2 cut(s) 395, 827
HpySE526I ACGT 3 cut(s) 645, 1301, 1521
Hsp92I GRCGYC 2 cut(s) 546, 1179
HspAI GCGC 2 cut(s) 546, 1371
KasI GGCGCC 1 cut(s) 545
Kpn2I TCCGGA 2 cut(s) 1232, 1245
KroI GCCGGC 2 cut(s) 542, 1550
KroNI GCCGGC 2 cut(s) 544, 1552
Ksp22I TGATCA 1 cut(s) 63
KspI CCGCGG 3 cut(s) 694, 777, 1308
Kzo9I GATC 6 cut(s) 63, 68, 270, 879, 1081, 1395
LguI GCTCTTC 1 cut(s) 733
LmnI GCTCC 3 cut(s) 245, 487, 1538
LweI GCATC 3 cut(s) 151, 1171, 1273
MaeI CTAG 7 cut(s) 36, 42, 437, 470, 476, 848, 1061
MaeII ACGT 3 cut(s) 645, 1301, 1521
MaeIII GTNAC 3 cut(s) 302, 913, 1146
MalI GATC 6 cut(s) 65, 70, 272, 881, 1083, 1397
MboI GATC 6 cut(s) 63, 68, 270, 879, 1081, 1395
MhlI GDGCHC 2 cut(s) 242, 785
MluCI AATT 4 cut(s) 84, 144, 450, 725
Mly113I GGCGCC 1 cut(s) 546
MlyI GAGTC 2 cut(s) 311, 393
MmeI TCCRAC 1 cut(s) 970
Mph1103I ATGCAT 1 cut(s) 166
MroI TCCGGA 2 cut(s) 1232, 1245
MroNI GCCGGC 2 cut(s) 542, 1550
MseI TTAA 1 cut(s) 1481
MslI CAYNNNNRTG 4 cut(s) 1028, 1030, 1187, 1269
MspA1I CMGCKG 6 cut(s) 77, 584, 693, 776, 1307, 1634
MspI CCGG 5 cut(s) 543, 1227, 1233, 1246, 1551
MspR9I CCNGG 5 cut(s) 754, 1228, 1359, 1393, 1446
Mva1269I GAATGC 1 cut(s) 1387
MvaI CCWGG 4 cut(s) 754, 1359, 1393, 1446
MvnI CGCG 4 cut(s) 315, 693, 776, 1307
NaeI GCCGGC 2 cut(s) 544, 1552
NarI GGCGCC 1 cut(s) 546
NciI CCSGG 1 cut(s) 1228
NcoI CCATGG 1 cut(s) 517
NdeI CATATG 1 cut(s) 884
NdeII GATC 6 cut(s) 63, 68, 270, 879, 1081, 1395
NgoMIV GCCGGC 2 cut(s) 542, 1550
NlaIV GGNNCC 5 cut(s) 547, 565, 705, 1231, 1540
NmeAIII GCCGAG 1 cut(s) 657
NmuCI GTSAC 2 cut(s) 302, 913
NsiI ATGCAT 1 cut(s) 166
NspI RCATGY 2 cut(s) 826, 1416
PaqCI CACCTGC 1 cut(s) 89
PciI ACATGT 1 cut(s) 1412
PciSI GCTCTTC 1 cut(s) 733
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PctI GAATGC 1 cut(s) 1387
PdiI GCCGGC 2 cut(s) 544, 1552
PfeI GAWTC 3 cut(s) 934, 1236, 1504
PfoI TCCNGGA 1 cut(s) 1391
Ple19I CGATCG 1 cut(s) 273
PleI GAGTC 2 cut(s) 311, 393
PluTI GGCGCC 1 cut(s) 549
PpsI GAGTC 2 cut(s) 311, 393
PscI ACATGT 1 cut(s) 1412
Psp6I CCWGG 4 cut(s) 752, 1357, 1391, 1444
PspGI CCWGG 4 cut(s) 752, 1357, 1391, 1444
PspN4I GGNNCC 5 cut(s) 547, 565, 705, 1231, 1540
PspPI GGNCC 5 cut(s) 564, 688, 704, 924, 1230
PstI CTGCAG 1 cut(s) 1161
PstNI CAGNNNCTG 1 cut(s) 1634
PvuI CGATCG 1 cut(s) 273
PvuII CAGCTG 2 cut(s) 584, 1634
RsaI GTAC 6 cut(s) 40, 46, 350, 392, 620, 1638
RsaNI GTAC 6 cut(s) 39, 45, 349, 391, 619, 1637
RseI CAYNNNNRTG 4 cut(s) 1028, 1030, 1187, 1269
SacII CCGCGG 3 cut(s) 694, 777, 1308
SapI GCTCTTC 1 cut(s) 733
SaqAI TTAA 1 cut(s) 1481
Sau3AI GATC 6 cut(s) 63, 68, 270, 879, 1081, 1395
Sau96I GGNCC 5 cut(s) 564, 688, 704, 924, 1230
ScaI AGTACT 2 cut(s) 40, 46
SchI GAGTC 2 cut(s) 311, 393
ScrFI CCNGG 5 cut(s) 754, 1228, 1359, 1393, 1446
SduI GDGCHC 2 cut(s) 242, 785
SfaNI GCATC 3 cut(s) 151, 1171, 1273
SfcI CTRYAG 1 cut(s) 1157
SfoI GGCGCC 1 cut(s) 547
Sfr303I CCGCGG 3 cut(s) 694, 777, 1308
SgrBI CCGCGG 3 cut(s) 694, 777, 1308
SinI GGWCC 4 cut(s) 564, 688, 704, 1230
SmiMI CAYNNNNRTG 4 cut(s) 1028, 1030, 1187, 1269
SmlI CTYRAG 1 cut(s) 1601
SmoI CTYRAG 1 cut(s) 1601
SpeI ACTAGT 1 cut(s) 41
Sse9I AATT 4 cut(s) 84, 144, 450, 725
SspDI GGCGCC 1 cut(s) 545
SspI AATATT 1 cut(s) 109
SspMI CTAG 7 cut(s) 36, 42, 437, 470, 476, 848, 1061
StyD4I CCNGG 5 cut(s) 752, 1226, 1357, 1391, 1444
StyI CCWWGG 1 cut(s) 517
TaaI ACNGT 4 cut(s) 49, 390, 618, 1489
TaiI ACGT 3 cut(s) 648, 1304, 1524
TaqI TCGA 1 cut(s) 81
TasI AATT 4 cut(s) 84, 144, 450, 725
TatI WGTACW 4 cut(s) 38, 44, 348, 1636
TauI GCSGC 7 cut(s) 365, 551, 557, 629, 776, 779, 1357
TfiI GAWTC 3 cut(s) 934, 1236, 1504
Tru1I TTAA 1 cut(s) 1481
Tru9I TTAA 1 cut(s) 1481
TscAI CASTG 3 cut(s) 898, 1161, 1492
TseFI GTSAC 2 cut(s) 302, 913
Tsp45I GTSAC 2 cut(s) 302, 913
TspDTI ATGAA 6 cut(s) 155, 238, 242, 444, 852, 1108
TspRI CASTG 3 cut(s) 898, 1161, 1492
VpaK11BI GGWCC 4 cut(s) 564, 688, 704, 1230
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 2 cut(s) 826, 1416
XspI CTAG 7 cut(s) 36, 42, 437, 470, 476, 848, 1061
ZrmI AGTACT 2 cut(s) 40, 46
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.