MD00G1034000.v1.1

cellular response to nitrogen starvation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0022361
Physical Location & Seq
Reverse (-)
5813916 .. 5815564
1649 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1034000.v1.1.491

Sequence Viewer

Length: 687 bp
ATGTCTGAAATTGATGATGCAAATAAGGGTGAAGATGGATTTCTTCACATGACCTATAGCGGGGGAGAAGAGAAAGGGATTGTTGAGTATGACGCTCAAATGTGTGACTTAATTCTTGGCGAGGTCGTCAAGATGAAGAAGAGGAAGATAAAAGGGGATTTTATTTTGTCAAAAGGCATTGTTATGTTAGACACTTGGAATGATCATCTTAACAAGGAGCATATGGCTCAGCTTGAGGAGAAGGCTGCGCTCGAGGGAAAACCTGCTGCTGAATTATCCACTTTTATCGGAAAGCATTTATGGGAGTTATGCCAAGAGCGTGAGCTAAATCCTTGGCTGTTGAGGAAGAAAAGACCAGATCATATACCAGTGAATGTGAAGAAGGATACAAGCAGTAAAATTCCTGACATTTGCATGAAGAAATTCCAAGTTCATTTCGATTTGCCTCTGAGCGAATTTATTGAGTTTATTCGGATTCGGATCGAGTGGAAAGAAGGAAGGCCATGGACAAAGCCTTTGTTTCTCTATTTCAAGAACACTGATCCTCCCGCTGGTACCTCGATGTATGTAATCGATAAGGCAAATAAGGATGACGACGGATTTCTTAACATCACCTACAGTGGAGAAGAGAAAGGAGCCTGGACGCAGTTAAACAACGGGGAGTTTGCTCATAAAGTAAAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg8

Protein Analysis

229

Amino Acids

26.33

Weight (kDa)

6.02

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 113 - 210 2.2e-15 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022361)

Species Orthologous Gene IDs
malus_domestica MD00G1034000.v1.1 MD03G1126000.v1.1 MD03G1126400.v1.1
pyrus_communis pycom10g27140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 271
Acc65I GGTACC 1 cut(s) 554
AccB1I GGYRCC 1 cut(s) 554
AciI CCGC 2 cut(s) 60, 549
AclWI GGATC 2 cut(s) 488, 536
AcsI RAATTY 3 cut(s) 399, 422, 455
AfaI GTAC 1 cut(s) 556
AfiI CCNNNNNNNGG 2 cut(s) 60, 551
AgsI TTSAA 1 cut(s) 532
AjnI CCWGG 1 cut(s) 638
AluBI AGCT 2 cut(s) 232, 325
AluI AGCT 2 cut(s) 232, 325
AlwI GGATC 2 cut(s) 488, 536
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 1 cut(s) 500
ApeKI GCWGC 2 cut(s) 245, 266
ApoI RAATTY 3 cut(s) 399, 422, 455
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
Asp700I GAANNNNTTC 1 cut(s) 422
Asp718I GGTACC 1 cut(s) 554
AspLEI GCGC 1 cut(s) 250
AsuHPI GGTGA 2 cut(s) 41, 604
AvaI CYCGRG 1 cut(s) 251
BanI GGYRCC 1 cut(s) 554
BbvI GCAGC 2 cut(s) 232, 253
BccI CCATC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 640
BciVI GTATCC 1 cut(s) 379
BclI TGATCA 1 cut(s) 202
BfmI CTRYAG 2 cut(s) 55, 616
BfuAI ACCTGC 1 cut(s) 271
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 2 cut(s) 246, 267
BlpI GCTNAGC 1 cut(s) 228
BlsI GCNGC 2 cut(s) 247, 268
Bme1390I CCNGG 1 cut(s) 640
BmeT110I CYCGRG 1 cut(s) 251
BmiI GGNNCC 2 cut(s) 556, 637
BmrFI CCNGG 1 cut(s) 640
BmsI GCATC 1 cut(s) 7
Bpu1102I GCTNAGC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 573
BsaBI GATNNNNATC 1 cut(s) 479
BsaJI CCNNGG 2 cut(s) 332, 503
BsaXI ACNNNNNCTCC 2 cut(s) 529, 559
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 551
Bse1I ACTGG 1 cut(s) 368
Bse8I GATNNNNATC 1 cut(s) 479
BseBI CCWGG 1 cut(s) 640
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 2 cut(s) 332, 503
BseGI GGATG 1 cut(s) 595
BseJI GATNNNNATC 1 cut(s) 479
BseLI CCNNNNNNNGG 2 cut(s) 60, 551
BseMII CTCAG 2 cut(s) 242, 440
BseNI ACTGG 1 cut(s) 368
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 2 cut(s) 232, 253
BshFI GGCC 1 cut(s) 502
BshNI GGYRCC 1 cut(s) 554
BshVI ATCGAT 1 cut(s) 573
BsiHKCI CYCGRG 1 cut(s) 251
BslI CCNNNNNNNGG 2 cut(s) 60, 551
BsnI GGCC 1 cut(s) 502
BsoBI CYCGRG 1 cut(s) 251
Bsp143I GATC 4 cut(s) 202, 358, 480, 541
Bsp1720I GCTNAGC 1 cut(s) 228
Bsp19I CCATGG 1 cut(s) 503
BspACI CCGC 2 cut(s) 60, 549
BspANI GGCC 1 cut(s) 502
BspCNI CTCAG 2 cut(s) 241, 441
BspDI ATCGAT 1 cut(s) 573
BspLI GGNNCC 2 cut(s) 556, 637
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 2 cut(s) 488, 536
BspT107I GGYRCC 1 cut(s) 554
BsrI ACTGG 1 cut(s) 368
BssECI CCNNGG 2 cut(s) 332, 503
BssMI GATC 4 cut(s) 202, 358, 480, 541
BssT1I CCWWGG 2 cut(s) 332, 503
Bst2UI CCWGG 1 cut(s) 640
Bst4CI ACNGT 1 cut(s) 620
Bst6I CTCTTC 3 cut(s) 63, 134, 621
BstDEI CTNAG 2 cut(s) 228, 449
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 1 cut(s) 595
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 4 cut(s) 205, 361, 483, 544
BstMBI GATC 4 cut(s) 202, 358, 480, 541
BstNI CCWGG 1 cut(s) 640
BstSCI CCNGG 1 cut(s) 638
BstSFI CTRYAG 2 cut(s) 55, 616
BstV1I GCAGC 2 cut(s) 232, 253
Bsu15I ATCGAT 1 cut(s) 573
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 1 cut(s) 502
BsuTUI ATCGAT 1 cut(s) 573
BtgI CCRYGG 1 cut(s) 503
BtsCI GGATG 1 cut(s) 595
BtsIMutI CAGTG 3 cut(s) 375, 537, 625
BveI ACCTGC 1 cut(s) 271
CfoI GCGC 1 cut(s) 250
ClaI ATCGAT 1 cut(s) 573
CseI GACGC 2 cut(s) 101, 652
Csp6I GTAC 1 cut(s) 555
CviAII CATG 3 cut(s) 49, 415, 504
CviJI RGCY 8 cut(s) 227, 232, 245, 325, 337, 502, 514, 638
CviKI_1 RGCY 8 cut(s) 227, 232, 245, 325, 337, 502, 514, 638
CviQI GTAC 1 cut(s) 555
DdeI CTNAG 2 cut(s) 228, 449
DpnI GATC 4 cut(s) 204, 360, 482, 543
DpnII GATC 4 cut(s) 202, 358, 480, 541
Eam1104I CTCTTC 3 cut(s) 63, 134, 621
EarI CTCTTC 3 cut(s) 63, 134, 621
Eco130I CCWWGG 2 cut(s) 332, 503
Eco88I CYCGRG 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 638
EcoT14I CCWWGG 2 cut(s) 332, 503
ErhI CCWWGG 2 cut(s) 332, 503
FaeI CATG 3 cut(s) 52, 418, 507
FatI CATG 3 cut(s) 48, 414, 503
FauI CCCGC 2 cut(s) 53, 556
FauNDI CATATG 1 cut(s) 222
FbaI TGATCA 1 cut(s) 202
Fnu4HI GCNGC 2 cut(s) 246, 267
FokI GGATG 1 cut(s) 602
Fsp4HI GCNGC 2 cut(s) 246, 267
GlaI GCGC 1 cut(s) 249
GluI GCNGC 2 cut(s) 246, 267
HaeIII GGCC 1 cut(s) 502
HgaI GACGC 2 cut(s) 101, 652
HhaI GCGC 1 cut(s) 250
Hin1II CATG 3 cut(s) 52, 418, 507
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HinfI GANTC 1 cut(s) 475
HphI GGTGA 2 cut(s) 41, 604
Hpy188I TCNGA 5 cut(s) 7, 290, 450, 474, 480
Hpy188III TCNNGA 3 cut(s) 130, 404, 532
Hpy99I CGWCG 1 cut(s) 599
HpyAV CCTTC 4 cut(s) 235, 376, 488, 492
HpyCH4III ACNGT 1 cut(s) 620
HpyCH4V TGCA 2 cut(s) 20, 414
HpyF3I CTNAG 2 cut(s) 228, 449
Hsp92II CATG 3 cut(s) 52, 418, 507
HspAI GCGC 1 cut(s) 248
KpnI GGTACC 1 cut(s) 558
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 4 cut(s) 202, 358, 480, 541
LmnI GCTCC 2 cut(s) 217, 635
LpnPI CCDG 7 cut(s) 276, 369, 381, 417, 537, 625, 652
Lsp1109I GCAGC 2 cut(s) 232, 253
LweI GCATC 1 cut(s) 7
MaeIII GTNAC 1 cut(s) 104
MalI GATC 4 cut(s) 204, 360, 482, 543
MboI GATC 4 cut(s) 202, 358, 480, 541
MluCI AATT 6 cut(s) 9, 111, 272, 399, 422, 455
MnlI CCTC 8 cut(s) 115, 135, 229, 247, 336, 456, 555, 568
MroXI GAANNNNTTC 1 cut(s) 422
MseI TTAA 4 cut(s) 110, 210, 606, 650
MslI CAYNNNNRTG 2 cut(s) 182, 413
MspA1I CMGCKG 1 cut(s) 551
MspR9I CCNGG 1 cut(s) 640
MvaI CCWGG 1 cut(s) 640
NcoI CCATGG 1 cut(s) 503
NdeI CATATG 1 cut(s) 222
NdeII GATC 4 cut(s) 202, 358, 480, 541
NlaIII CATG 3 cut(s) 52, 418, 507
NlaIV GGNNCC 2 cut(s) 556, 637
NmuCI GTSAC 1 cut(s) 104
PaeR7I CTCGAG 1 cut(s) 251
PdmI GAANNNNTTC 1 cut(s) 422
PfeI GAWTC 1 cut(s) 475
PkrI GCNGC 2 cut(s) 247, 268
Psp6I CCWGG 1 cut(s) 638
PspGI CCWGG 1 cut(s) 638
PspN4I GGNNCC 2 cut(s) 556, 637
PspXI VCTCGAGB 1 cut(s) 251
RsaI GTAC 1 cut(s) 556
RsaNI GTAC 1 cut(s) 555
RseI CAYNNNNRTG 2 cut(s) 182, 413
SaqAI TTAA 4 cut(s) 110, 210, 606, 650
SatI GCNGC 2 cut(s) 246, 267
Sau3AI GATC 4 cut(s) 202, 358, 480, 541
ScrFI CCNGG 1 cut(s) 640
SetI ASST 7 cut(s) 56, 126, 234, 265, 327, 560, 617
SfaNI GCATC 1 cut(s) 7
SfcI CTRYAG 2 cut(s) 55, 616
Sfr274I CTCGAG 1 cut(s) 251
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 2 cut(s) 182, 413
SmlI CTYRAG 2 cut(s) 233, 251
SmoI CTYRAG 2 cut(s) 233, 251
Sse9I AATT 6 cut(s) 9, 111, 272, 399, 422, 455
SsiI CCGC 2 cut(s) 60, 549
StyD4I CCNGG 1 cut(s) 638
StyI CCWWGG 2 cut(s) 332, 503
TaaI ACNGT 1 cut(s) 620
TaqI TCGA 5 cut(s) 252, 438, 483, 560, 573
TasI AATT 6 cut(s) 9, 111, 272, 399, 422, 455
TfiI GAWTC 1 cut(s) 475
Tru1I TTAA 4 cut(s) 110, 210, 606, 650
Tru9I TTAA 4 cut(s) 110, 210, 606, 650
TscAI CASTG 3 cut(s) 375, 544, 625
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 2 cut(s) 245, 266
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 3 cut(s) 149, 422, 431
TspGWI ACGGA 1 cut(s) 612
TspRI CASTG 3 cut(s) 375, 544, 625
XapI RAATTY 3 cut(s) 399, 422, 455
XhoI CTCGAG 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.