MD03G1126000.v1.1

cellular response to nitrogen starvation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0022361
Physical Location & Seq
Forward (+)
11888289 .. 11888890
602 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1126000.v1.1.491

Sequence Viewer

Length: 399 bp
ATGTTCAGGTCATTTTTGGAGGAGTACAATGAATACACTATAAATGCTTGGAAATTGAAGGATAGCCAACCAGATCTTGTCCCAGTAAGGGTTCGCACACAGATTTATCGCCTATACTGTGTTTGTGACAGCAGATTCCAAGTTCATTTCGATTTGCCTCTGAGCGAATTTATTGAGTTTATTCGGATTCGGATCGAGTGGAAAGAAGGAAGGCCATGGACAAAGCCTTTGTTTTTCTATTTCAAGAACACTGATCCTCCCGCTGGTACATCTATGTATGTAATCAATGAGGAAAATAAGGATGACGACGGATTTCTTAACATCACCTACAGTGGAGAAGAGAAAGGAGCCTGGACGGAGTTAAAGAACAAGGAGTTTGCTCATAAAGTAAAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg8

Protein Analysis

133

Amino Acids

15.92

Weight (kDa)

5.75

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 16 - 114 9.3e-11 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022361)

Species Orthologous Gene IDs
malus_domestica MD00G1034000.v1.1 MD03G1126000.v1.1 MD03G1126400.v1.1
pyrus_communis pycom10g27140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 261
AclWI GGATC 2 cut(s) 200, 248
AcsI RAATTY 1 cut(s) 167
AfaI GTAC 2 cut(s) 26, 268
AfiI CCNNNNNNNGG 2 cut(s) 88, 263
AgsI TTSAA 2 cut(s) 58, 244
AjnI CCWGG 1 cut(s) 350
AlwI GGATC 2 cut(s) 200, 248
AoxI GGCC 1 cut(s) 212
ApoI RAATTY 1 cut(s) 167
ArsI GACNNNNNNTTYG 2 cut(s) 211, 243
AsuHPI GGTGA 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 352
BfmI CTRYAG 1 cut(s) 328
BglII AGATCT 1 cut(s) 73
Bme1390I CCNGG 1 cut(s) 352
BmiI GGNNCC 1 cut(s) 349
BmrFI CCNGG 1 cut(s) 352
BmrI ACTGGG 1 cut(s) 77
BmuI ACTGGG 1 cut(s) 77
BsaBI GATNNNNATC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 263
Bse1I ACTGG 1 cut(s) 83
Bse8I GATNNNNATC 1 cut(s) 191
BseBI CCWGG 1 cut(s) 352
BseDI CCNNGG 1 cut(s) 215
BseGI GGATG 1 cut(s) 307
BseJI GATNNNNATC 1 cut(s) 191
BseLI CCNNNNNNNGG 2 cut(s) 88, 263
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 1 cut(s) 83
BseRI GAGGAG 1 cut(s) 35
BshFI GGCC 1 cut(s) 214
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 2 cut(s) 88, 263
BsmFI GGGAC 1 cut(s) 65
BsnI GGCC 1 cut(s) 214
Bsp143I GATC 3 cut(s) 73, 192, 253
Bsp19I CCATGG 1 cut(s) 215
BspACI CCGC 1 cut(s) 261
BspANI GGCC 1 cut(s) 214
BspCNI CTCAG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 349
BspPI GGATC 2 cut(s) 200, 248
BsrI ACTGG 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 215
BssMI GATC 3 cut(s) 73, 192, 253
BssT1I CCWWGG 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 352
Bst4CI ACNGT 2 cut(s) 119, 332
Bst6I CTCTTC 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 161
BstDSI CCRYGG 1 cut(s) 215
BstF5I GGATG 1 cut(s) 307
BstKTI GATC 3 cut(s) 76, 195, 256
BstMBI GATC 3 cut(s) 73, 192, 253
BstNI CCWGG 1 cut(s) 352
BstSCI CCNGG 1 cut(s) 350
BstSFI CTRYAG 1 cut(s) 328
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 1 cut(s) 214
BtgI CCRYGG 1 cut(s) 215
BtsCI GGATG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 249, 337
Csp6I GTAC 2 cut(s) 25, 267
CviAII CATG 1 cut(s) 216
CviJI RGCY 4 cut(s) 66, 214, 226, 350
CviKI_1 RGCY 4 cut(s) 66, 214, 226, 350
CviQI GTAC 2 cut(s) 25, 267
DdeI CTNAG 1 cut(s) 161
DpnI GATC 3 cut(s) 75, 194, 255
DpnII GATC 3 cut(s) 73, 192, 253
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 215
FaeI CATG 1 cut(s) 219
FaiI YATR 7 cut(s) 41, 115, 217, 275, 279, 384, 397
FaqI GGGAC 1 cut(s) 65
FatI CATG 1 cut(s) 215
FauI CCCGC 1 cut(s) 268
FokI GGATG 1 cut(s) 314
HaeIII GGCC 1 cut(s) 214
Hin1II CATG 1 cut(s) 219
HinfI GANTC 2 cut(s) 135, 187
HphI GGTGA 1 cut(s) 316
Hpy188I TCNGA 3 cut(s) 162, 186, 192
Hpy188III TCNNGA 1 cut(s) 244
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 3 cut(s) 52, 200, 204
HpyCH4III ACNGT 2 cut(s) 119, 332
HpyF3I CTNAG 1 cut(s) 161
Hsp92II CATG 1 cut(s) 219
Kzo9I GATC 3 cut(s) 73, 192, 253
LmnI GCTCC 1 cut(s) 347
LpnPI CCDG 5 cut(s) 84, 96, 249, 337, 364
MaeIII GTNAC 1 cut(s) 125
MalI GATC 3 cut(s) 75, 194, 255
MboI GATC 3 cut(s) 73, 192, 253
MboII GAAGA 1 cut(s) 350
MflI RGATCY 1 cut(s) 73
MluCI AATT 2 cut(s) 53, 167
MnlI CCTC 4 cut(s) 13, 168, 267, 283
MseI TTAA 2 cut(s) 318, 362
MspA1I CMGCKG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 352
MvaI CCWGG 1 cut(s) 352
NcoI CCATGG 1 cut(s) 215
NdeII GATC 3 cut(s) 73, 192, 253
NlaIII CATG 1 cut(s) 219
NlaIV GGNNCC 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 135, 187
Psp6I CCWGG 1 cut(s) 350
PspGI CCWGG 1 cut(s) 350
PspN4I GGNNCC 1 cut(s) 349
PsuI RGATCY 1 cut(s) 73
RsaI GTAC 2 cut(s) 26, 268
RsaNI GTAC 2 cut(s) 25, 267
SaqAI TTAA 2 cut(s) 318, 362
Sau3AI GATC 3 cut(s) 73, 192, 253
ScrFI CCNGG 1 cut(s) 352
SetI ASST 2 cut(s) 11, 329
SfcI CTRYAG 1 cut(s) 328
Sse9I AATT 2 cut(s) 53, 167
SsiI CCGC 1 cut(s) 261
StyD4I CCNGG 1 cut(s) 350
StyI CCWWGG 1 cut(s) 215
TaaI ACNGT 2 cut(s) 119, 332
TaqI TCGA 2 cut(s) 150, 195
TasI AATT 2 cut(s) 53, 167
TatI WGTACW 1 cut(s) 24
TfiI GAWTC 2 cut(s) 135, 187
Tru1I TTAA 2 cut(s) 318, 362
Tru9I TTAA 2 cut(s) 318, 362
TscAI CASTG 2 cut(s) 256, 337
TseFI GTSAC 1 cut(s) 125
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 2 cut(s) 45, 134
TspGWI ACGGA 2 cut(s) 324, 371
TspRI CASTG 2 cut(s) 256, 337
XapI RAATTY 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.