MD00G1103700.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0017055
Physical Location & Seq
Forward (+)
21814328 .. 21816049
1722 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1103700.v1.1.491

Sequence Viewer

Length: 1722 bp
ATGGCAGAAAGAAACCGATACACGATTCAATCCCCTCTCTTGTTTATTATGTGCCTTCTCGTTCCTTGTTTAGCTGAATTTCCTGCGGACCAAATCACTTTTTGTTTGACGCTTCATGACATCAACAACTTTACCACATTCCCTAACACGGAAAATGAGTCTGCAGCTTACTACAAGCTGCTCAACTTTTCCATCCAAAATCTTCGTTTCGCTGATCCTGCCCTTCCTAAGCCAGTAGCCATCATACTTCCCGAGAGCGTTGAGCAGCTAGTAAACTCTGTGTCATGCTCTAGACAAGGGTTGTTGGAAATCAGAGTAAGGAGTGGTGGACACAGCTACGAAGGGACATCATCCGTTGCTGTAGATGGAGCTCTGTTTGTGATCATCGACATGATGAACTTGAACGGTGTGTCGGTGGATTTGGAAACGGAAACTGCATGGGTGGAAGGAGGTGCAACGCTTGGTGAGACATACCATGCGATTGCCCAAGCAAGCAATCTTCATGGGTTCTCAGCCGGGTCATGCCCAACTGTAGGCGCTGGAGGCCATATTGCTGGTGGTGGATTCGGCCTCATGTCGAGGAAATACGGACTTGCAGCCGATAACGTGGTGGATGCACTACTTGTTGATGCAAACGGACAACTGTTAGACCGACAAGGCATGGGAGAGGACGTGTTTTGGGCAATTAGAGGAGGTGGTGGGGGTGTTTTCGGGATTGTCTATGCTTGGAAAATCCAGTTGTTGAATGTGCCACAAGTCGTAACTGCTTTCGTAGTGTCTAGAGCTGCTTCTGCTGCTGCAGGAGGAGGAGGCAACGAAATCTTTAATCATAATGTAGCAAGGCTGGTAGACAAGTGGCAACACATCGCACCTTACTTGGAAGACGATTTCTACATTTCGTGCTTTGTCGGCGCTGGGTTGCCAGAGGTCAAGACTACTACTGTTACTGCTACTAGTAGTACTAATATATCAGCTACGTTCAAGGGGTTTTATCTGGGTCCAAGAAGCAGCGCCATGTCCATCCTAAACAAAGTTTTTCCTGACTTGGGTGTTGCAGAAGAAGATTGCGCAGAAATGAGTTGGATCGAGTCCATCGTGTTCTTCTCCGGCCTAAGTAAGGGAAGCTCGGTGTCCGACTTGAGAAATCGATATCTGCAAGGCAAGGGATTTTTCAAAGCGAAATCGGATTACGTTAGGACCCCAATTTCTTACAGAGGGATAAGGGCAGCCCTGGAAATACTTGAGGAGGAGCCGAAAGGGTACGTGATTTTAGATCCTTATGGTGGGGTTATGCATGATATAAGCAGCGAAGCCATTGCATTTCCTCACAGAAAAGGTAACCTCTTTACAATTCAGTACCTGGTGGAATGGAAAGAAGAAGATAACCACAAAATAAATGACTACATAGAATGGATAAGAAGATTCCACAATGCAATGACAGAGTATGTTTCGTGGGGTCCTAGGGCTGCTTATATCAACTATGTTGACCTCGACCTCGGAGTGATGATGCGGCAGCAGTTTCCTTTAAATAATAATTTGGTTAAGGATGATGAATCTGACGCTGTGGAAATTGCCAGGAATTGGGGTGAAAAGTACTTCCTAAATAACTACGACAGATTGGTGAGAGCAAAAACACTTATTGATCCAACCAACGTTTTCAGGAATCAGCAAGGGATTCCTCCGATGTCGTCGTCTTCGTCTTTAGTGGTGCTCGATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

574

Amino Acids

63.02

Weight (kDa)

5.16

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 79 - 216 1.6e-26 FAD binding domain
BBE PF08031 490 - 560 2.6e-18 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44380 AT5G44380
fragaria_vesca FvH4_2g00110
malus_domestica MD00G1103700.v1.1
prunus_persica Prupe.4G257400_v2.0.a1
pyrus_communis pycom10g00010
rosa_chinensis RchiOBHm_Chr6g0249371
rosa_laevigata RLG00000015586
rosa_multiflora Rmu_co8327045.1_g000001 Rmu_sc0006234.1_g000001
rosa_roxburghii Rroxscaffold_7G00217810
rosa_rugosa Rorug05G0491200
rosa_wichuraiana Rw6G000210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1069
AccB7I CCANNNNNTGG 1 cut(s) 1581
AccI GTMKAC 1 cut(s) 849
AciI CCGC 2 cut(s) 86, 1510
AclI AACGTT 1 cut(s) 1653
AclWI GGATC 4 cut(s) 209, 1091, 1268, 1637
AcsI RAATTY 1 cut(s) 77
AfaI GTAC 4 cut(s) 961, 1262, 1358, 1595
AfiI CCNNNNNNNGG 6 cut(s) 148, 533, 1046, 1117, 1283, 1581
AflIII ACRYGT 1 cut(s) 672
AgsI TTSAA 5 cut(s) 29, 403, 745, 982, 1174
AhlI ACTAGT 1 cut(s) 953
AjiI CACGTC 1 cut(s) 673
AjnI CCWGG 3 cut(s) 1230, 1359, 1574
AluBI AGCT 9 cut(s) 74, 167, 178, 268, 336, 371, 785, 974, 1125
AluI AGCT 9 cut(s) 74, 167, 178, 268, 336, 371, 785, 974, 1125
Alw21I GWGCWC 2 cut(s) 373, 1713
Alw26I GTCTC 1 cut(s) 461
AlwI GGATC 4 cut(s) 209, 1091, 1268, 1637
AlwNI CAGNNNCTG 1 cut(s) 1360
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 3 cut(s) 544, 568, 1108
ApoI RAATTY 1 cut(s) 77
AspA2I CCTAGG 1 cut(s) 1460
AspLEI GCGC 4 cut(s) 539, 914, 1013, 1070
AspS9I GGNCC 4 cut(s) 88, 998, 1197, 1457
AsuC2I CCSGG 1 cut(s) 517
AsuHPI GGTGA 3 cut(s) 476, 1598, 1633
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 4 cut(s) 88, 998, 1197, 1457
AvrII CCTAGG 1 cut(s) 1460
BanII GRGCYC 1 cut(s) 373
BbsI GAAGAC 2 cut(s) 888, 1686
Bbv12I GWGCWC 2 cut(s) 373, 1713
BccI CCATC 5 cut(s) 200, 248, 359, 1028, 1100
BcgI CGANNNNNNTGC 2 cut(s) 1298, 1332
BciT130I CCWGG 3 cut(s) 1232, 1361, 1576
BclI TGATCA 1 cut(s) 381
BcnI CCSGG 1 cut(s) 517
BcoDI GTCTC 1 cut(s) 461
BcuI ACTAGT 1 cut(s) 953
BfaI CTAG 5 cut(s) 269, 291, 780, 954, 1461
BfmI CTRYAG 4 cut(s) 162, 360, 531, 798
BfoI RGCGCY 3 cut(s) 540, 915, 1014
BlnI CCTAGG 1 cut(s) 1460
BmcAI AGTACT 2 cut(s) 961, 1595
Bme1390I CCNGG 4 cut(s) 517, 1232, 1361, 1576
Bme18I GGWCC 4 cut(s) 88, 998, 1197, 1457
BmeT110I CYCGRG 1 cut(s) 251
BmgBI CACGTC 1 cut(s) 673
BmgT120I GGNCC 4 cut(s) 88, 998, 1197, 1457
BmiI GGNNCC 4 cut(s) 999, 1199, 1251, 1458
BmrFI CCNGG 4 cut(s) 517, 1232, 1361, 1576
BmsI GCATC 3 cut(s) 604, 619, 1497
BpiI GAAGAC 2 cut(s) 888, 1686
BpmI CTGGAG 1 cut(s) 561
Bpu10I CCTNAGC 1 cut(s) 228
BpuEI CTTGAG 2 cut(s) 1159, 1262
BpuMI CCSGG 1 cut(s) 517
Bsa29I ATCGAT 1 cut(s) 1147
BsaAI YACGTR 1 cut(s) 1264
BsaJI CCNNGG 3 cut(s) 1230, 1460, 1495
BsaXI ACNNNNNCTCC 2 cut(s) 657, 687
Bsc4I CCNNNNNNNGG 6 cut(s) 148, 533, 1046, 1117, 1283, 1581
Bse1I ACTGG 2 cut(s) 233, 736
Bse3DI GCAATG 2 cut(s) 1314, 1440
BseBI CCWGG 3 cut(s) 1232, 1361, 1576
BseCI ATCGAT 1 cut(s) 1147
BseDI CCNNGG 3 cut(s) 1230, 1460, 1495
BseGI GGATG 5 cut(s) 192, 350, 619, 1020, 1552
BseLI CCNNNNNNNGG 6 cut(s) 148, 533, 1046, 1117, 1283, 1581
BseMI GCAATG 2 cut(s) 1314, 1440
BseMII CTCAG 1 cut(s) 525
BseNI ACTGG 2 cut(s) 233, 736
BseRI GAGGAG 5 cut(s) 705, 819, 822, 1259, 1262
BseYI CCCAGC 1 cut(s) 914
BshFI GGCC 3 cut(s) 546, 570, 1110
BshVI ATCGAT 1 cut(s) 1147
BsiHKAI GWGCWC 2 cut(s) 373, 1713
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 2 cut(s) 516, 1107
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 6 cut(s) 148, 533, 1046, 1117, 1283, 1581
BsmAI GTCTC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 3 cut(s) 546, 570, 1110
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 2 cut(s) 373, 1713
Bsp143I GATC 6 cut(s) 214, 381, 1083, 1273, 1642, 1714
BspACI CCGC 2 cut(s) 86, 1510
BspANI GGCC 3 cut(s) 546, 570, 1110
BspCNI CTCAG 1 cut(s) 524
BspDI ATCGAT 1 cut(s) 1147
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 4 cut(s) 999, 1199, 1251, 1458
BspMAI CTGCAG 2 cut(s) 166, 802
BspPI GGATC 4 cut(s) 209, 1091, 1268, 1637
BsrDI GCAATG 2 cut(s) 1314, 1440
BsrI ACTGG 2 cut(s) 233, 736
BssECI CCNNGG 3 cut(s) 1230, 1460, 1495
BssMI GATC 6 cut(s) 214, 381, 1083, 1273, 1642, 1714
BssT1I CCWWGG 1 cut(s) 1460
Bst2UI CCWGG 3 cut(s) 1232, 1361, 1576
Bst4CI ACNGT 4 cut(s) 407, 532, 645, 943
BstBAI YACGTR 1 cut(s) 1264
BstC8I GCNNGC 1 cut(s) 493
BstDEI CTNAG 3 cut(s) 228, 511, 1112
BstEII GGTNACC 1 cut(s) 1337
BstENI CCTNNNNNAGG 1 cut(s) 1115
BstF5I GGATG 5 cut(s) 192, 350, 619, 1020, 1552
BstH2I RGCGCY 3 cut(s) 540, 915, 1014
BstHHI GCGC 4 cut(s) 539, 914, 1013, 1070
BstKTI GATC 6 cut(s) 217, 384, 1086, 1276, 1645, 1717
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 6 cut(s) 214, 381, 1083, 1273, 1642, 1714
BstMWI GCNNNNNNNGC 5 cut(s) 218, 543, 791, 794, 909
BstNI CCWGG 3 cut(s) 1232, 1361, 1576
BstPI GGTNACC 1 cut(s) 1337
BstSCI CCNGG 4 cut(s) 515, 1230, 1359, 1574
BstSFI CTRYAG 4 cut(s) 162, 360, 531, 798
BstV2I GAAGAC 2 cut(s) 888, 1686
BstX2I RGATCY 1 cut(s) 1273
BstXI CCANNNNNNTGG 1 cut(s) 554
BstYI RGATCY 1 cut(s) 1273
Bsu15I ATCGAT 1 cut(s) 1147
BsuRI GGCC 3 cut(s) 546, 570, 1110
BsuTUI ATCGAT 1 cut(s) 1147
BtgZI GCGATG 1 cut(s) 850
BtrI CACGTC 1 cut(s) 673
BtsCI GGATG 5 cut(s) 192, 350, 619, 1020, 1552
Cac8I GCNNGC 1 cut(s) 493
CaiI CAGNNNCTG 1 cut(s) 1360
CciI TCATGA 1 cut(s) 115
CfoI GCGC 4 cut(s) 539, 914, 1013, 1070
Cfr13I GGNCC 4 cut(s) 88, 998, 1197, 1457
ClaI ATCGAT 1 cut(s) 1147
CseI GACGC 2 cut(s) 118, 1568
CsiI ACCWGGT 1 cut(s) 1359
Csp6I GTAC 4 cut(s) 960, 1261, 1357, 1594
CviQI GTAC 4 cut(s) 960, 1261, 1357, 1594
DdeI CTNAG 3 cut(s) 228, 511, 1112
DpnI GATC 6 cut(s) 216, 383, 1085, 1275, 1644, 1716
DpnII GATC 6 cut(s) 214, 381, 1083, 1273, 1642, 1714
DraI TTTAAA 1 cut(s) 1527
Ecl136II GAGCTC 1 cut(s) 371
Eco130I CCWWGG 1 cut(s) 1460
Eco24I GRGCYC 1 cut(s) 373
Eco32I GATATC 1 cut(s) 1151
Eco47I GGWCC 4 cut(s) 88, 998, 1197, 1457
Eco53kI GAGCTC 1 cut(s) 371
Eco88I CYCGRG 1 cut(s) 251
Eco91I GGTNACC 1 cut(s) 1337
EcoICRI GAGCTC 1 cut(s) 371
EcoNI CCTNNNNNAGG 1 cut(s) 1115
EcoO109I RGGNCCY 2 cut(s) 1197, 1457
EcoO65I GGTNACC 1 cut(s) 1337
EcoRII CCWGG 3 cut(s) 1230, 1359, 1574
EcoRV GATATC 1 cut(s) 1151
EcoT14I CCWWGG 1 cut(s) 1460
EcoT22I ATGCAT 1 cut(s) 1296
EcoT38I GRGCYC 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 1460
FaqI GGGAC 1 cut(s) 358
FbaI TGATCA 1 cut(s) 381
FblI GTMKAC 1 cut(s) 849
FokI GGATG 5 cut(s) 179, 337, 626, 1007, 1559
FriOI GRGCYC 1 cut(s) 373
FspBI CTAG 5 cut(s) 269, 291, 780, 954, 1461
FspI TGCGCA 1 cut(s) 1069
GlaI GCGC 4 cut(s) 538, 913, 1012, 1069
GsaI CCCAGC 1 cut(s) 918
GsuI CTGGAG 1 cut(s) 561
HaeII RGCGCY 3 cut(s) 540, 915, 1014
HaeIII GGCC 3 cut(s) 546, 570, 1110
HapII CCGG 2 cut(s) 516, 1107
HgaI GACGC 2 cut(s) 118, 1568
HhaI GCGC 4 cut(s) 539, 914, 1013, 1070
Hin6I GCGC 4 cut(s) 537, 912, 1011, 1068
HinP1I GCGC 4 cut(s) 537, 912, 1011, 1068
HincII GTYRAC 1 cut(s) 1486
HindII GTYRAC 1 cut(s) 1486
HinfI GANTC 8 cut(s) 25, 158, 564, 1088, 1422, 1553, 1663, 1675
HpaII CCGG 2 cut(s) 516, 1107
HphI GGTGA 3 cut(s) 476, 1598, 1633
Hpy166II GTNNAC 4 cut(s) 274, 329, 850, 1486
Hpy188I TCNGA 6 cut(s) 314, 1135, 1186, 1499, 1558, 1683
Hpy188III TCNNGA 8 cut(s) 116, 251, 291, 712, 780, 931, 1040, 1660
Hpy8I GTNNAC 4 cut(s) 274, 329, 850, 1486
Hpy99I CGWCG 1 cut(s) 1693
HpyAV CCTTC 4 cut(s) 65, 233, 335, 440
HpyCH4III ACNGT 4 cut(s) 407, 532, 645, 943
HpyCH4IV ACGT 6 cut(s) 606, 672, 977, 1191, 1263, 1653
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 543, 791, 794, 909
HpyF3I CTNAG 3 cut(s) 228, 511, 1112
HpySE526I ACGT 6 cut(s) 606, 672, 977, 1191, 1263, 1653
HspAI GCGC 4 cut(s) 537, 912, 1011, 1068
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 6 cut(s) 214, 381, 1083, 1273, 1642, 1714
LmnI GCTCC 2 cut(s) 368, 1249
LweI GCATC 3 cut(s) 604, 619, 1497
MabI ACCWGGT 1 cut(s) 1359
MaeI CTAG 5 cut(s) 269, 291, 780, 954, 1461
MaeII ACGT 6 cut(s) 606, 672, 977, 1191, 1263, 1653
MaeIII GTNAC 3 cut(s) 760, 943, 1337
MalI GATC 6 cut(s) 216, 383, 1085, 1275, 1644, 1716
MboI GATC 6 cut(s) 214, 381, 1083, 1273, 1642, 1714
MflI RGATCY 1 cut(s) 1273
MhlI GDGCHC 2 cut(s) 373, 1713
MluCI AATT 7 cut(s) 77, 684, 1203, 1350, 1534, 1569, 1579
MlyI GAGTC 2 cut(s) 167, 1097
MmeI TCCRAC 4 cut(s) 285, 1061, 1158, 1670
Mph1103I ATGCAT 1 cut(s) 1296
MseI TTAA 3 cut(s) 825, 1526, 1542
MslI CAYNNNNRTG 1 cut(s) 389
MspI CCGG 2 cut(s) 516, 1107
MspR9I CCNGG 4 cut(s) 517, 1232, 1361, 1576
MvaI CCWGG 3 cut(s) 1232, 1361, 1576
MwoI GCNNNNNNNGC 5 cut(s) 218, 543, 791, 794, 909
NciI CCSGG 1 cut(s) 517
NdeII GATC 6 cut(s) 214, 381, 1083, 1273, 1642, 1714
NlaIV GGNNCC 4 cut(s) 999, 1199, 1251, 1458
NsbI TGCGCA 1 cut(s) 1069
NsiI ATGCAT 1 cut(s) 1296
PagI TCATGA 1 cut(s) 115
PcsI WCGNNNNNNNCGW 2 cut(s) 1092, 1694
PfeI GAWTC 6 cut(s) 25, 564, 1422, 1553, 1663, 1675
PflMI CCANNNNNTGG 1 cut(s) 1581
PleI GAGTC 2 cut(s) 166, 1096
PpsI GAGTC 2 cut(s) 166, 1096
Ppu21I YACGTR 1 cut(s) 1264
PpuMI RGGWCCY 2 cut(s) 1197, 1457
Psp124BI GAGCTC 1 cut(s) 373
Psp1406I AACGTT 1 cut(s) 1653
Psp5II RGGWCCY 2 cut(s) 1197, 1457
Psp6I CCWGG 3 cut(s) 1230, 1359, 1574
PspEI GGTNACC 1 cut(s) 1337
PspFI CCCAGC 1 cut(s) 914
PspGI CCWGG 3 cut(s) 1230, 1359, 1574
PspN4I GGNNCC 4 cut(s) 999, 1199, 1251, 1458
PspPI GGNCC 4 cut(s) 88, 998, 1197, 1457
PspPPI RGGWCCY 2 cut(s) 1197, 1457
PstI CTGCAG 2 cut(s) 166, 802
PstNI CAGNNNCTG 1 cut(s) 1360
PsuI RGATCY 1 cut(s) 1273
RsaI GTAC 4 cut(s) 961, 1262, 1358, 1595
RsaNI GTAC 4 cut(s) 960, 1261, 1357, 1594
RseI CAYNNNNRTG 1 cut(s) 389
SacI GAGCTC 1 cut(s) 373
SaqAI TTAA 3 cut(s) 825, 1526, 1542
Sau3AI GATC 6 cut(s) 214, 381, 1083, 1273, 1642, 1714
Sau96I GGNCC 4 cut(s) 88, 998, 1197, 1457
ScaI AGTACT 2 cut(s) 961, 1595
SchI GAGTC 2 cut(s) 167, 1097
ScrFI CCNGG 4 cut(s) 517, 1232, 1361, 1576
SduI GDGCHC 2 cut(s) 373, 1713
SexAI ACCWGGT 1 cut(s) 1359
SfaNI GCATC 3 cut(s) 604, 619, 1497
SfcI CTRYAG 4 cut(s) 162, 360, 531, 798
SinI GGWCC 4 cut(s) 88, 998, 1197, 1457
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 2 cut(s) 1138, 1241
SmoI CTYRAG 2 cut(s) 1138, 1241
SpeI ACTAGT 1 cut(s) 953
Sse9I AATT 7 cut(s) 77, 684, 1203, 1350, 1534, 1569, 1579
SsiI CCGC 2 cut(s) 86, 1510
SspMI CTAG 5 cut(s) 269, 291, 780, 954, 1461
SstI GAGCTC 1 cut(s) 373
StyD4I CCNGG 4 cut(s) 515, 1230, 1359, 1574
StyI CCWWGG 1 cut(s) 1460
TaaI ACNGT 4 cut(s) 407, 532, 645, 943
TaiI ACGT 6 cut(s) 609, 675, 980, 1194, 1266, 1656
TaqI TCGA 6 cut(s) 387, 578, 1086, 1147, 1491, 1713
TaqII GACCGA 1 cut(s) 666
TasI AATT 7 cut(s) 77, 684, 1203, 1350, 1534, 1569, 1579
TatI WGTACW 2 cut(s) 959, 1593
TauI GCSGC 1 cut(s) 1513
TfiI GAWTC 6 cut(s) 25, 564, 1422, 1553, 1663, 1675
Tru1I TTAA 3 cut(s) 825, 1526, 1542
Tru9I TTAA 3 cut(s) 825, 1526, 1542
TspDTI ATGAA 4 cut(s) 104, 410, 491, 1566
TspGWI ACGGA 5 cut(s) 164, 343, 443, 603, 651
Van91I CCANNNNNTGG 1 cut(s) 1581
VpaK11BI GGWCC 4 cut(s) 88, 998, 1197, 1457
XagI CCTNNNNNAGG 1 cut(s) 1115
XapI RAATTY 1 cut(s) 77
XbaI TCTAGA 2 cut(s) 290, 779
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
XmaJI CCTAGG 1 cut(s) 1460
XmiI GTMKAC 1 cut(s) 849
XspI CTAG 5 cut(s) 269, 291, 780, 954, 1461
ZrmI AGTACT 2 cut(s) 961, 1595
Zsp2I ATGCAT 1 cut(s) 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.