Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0012452

Physical Location & Seq

Genomic Coordinates Reverse (-)

25675895 .. 25681347

Gene Structure 5453 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1120100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1959 bp

ATGGCTACTTCGGAAGTTGTTCGACAAGTTATGTCTGGGGCATTACCTCGACTGGGCTGTTTTGATATATGTTTGAGCAATATGAATGGAGTTGTTTCTGTTAAGCCCGGTGTTAATAATGCACGGAAAATAAGTTCACTGAGTGCCCAAGTGCATAAAAGTTCGAGAGTTTTGAGGGACCGCAGGAGGATATGTGCGTTGCAGGGGAGAAATGATGTTTTTCATGGAAAGGGTCGTGTCCATAGGTTGGATTCAATGAGTTGCAAATGTCAACAGGCAGAAAGTTTAAGAGGGGCAACTGAAGAAGATCAGCACGGGGCAGTTTTCGTAGACAAATCAGATAAAGCAGTATCAGTTCCTCCTAATGGTATAACTAGTCCAGATATTGATGAATTGAAGGTGGATCAACAACTGAATCAGGAAGTGGGACGTTTTGGGTCAAATACCAAGCCGGCTGCAGCTAGAAAAAAGAAAGGTTCTACATGGAAGTCTAAGGCTGATTTGATTGAGGAGGAAGCATGGACCTTACTAAAGAACTCCATGGTCTATTATTGTAACAGTCCTGTTGGAACCATTGCCGCTAATGATCCAAGCTCTCCTAGTACCCTTAACTATGATCAGGTCTTTATTCGTGACTTCATACCTTCTGGGATAGCTTTCCTTTTAAAGGGGGAGTATGATATTGTGCGGAACTTCATTCTTCATACCCTTCAGTTGCAGAGCTGGGAGAAAACCATGGATTGTTATAGTCCTGGTCAAGGACTAATGCCCGCCAGTTTCAAGGTTCGCACTGTTCCACTTGATGGTGATGAGTTTGCAACTGAAGATGTATTGGATCCTGACTTTGGTGAAGCTGCTATTGGTCGTGTTGCCCCAGTTGACTCTGGGTTATGGTGGATTATATTGTTACGAGCTTATGGAAAGTGCTCCGGAGATCTTTCAGTGCAGGAAAGAGTTGATGTGCAAACGGGGATAAAAATGATTTTAAAGCTATGTCTTGCTGATGGTTTTGATATGTTTCCAACATTATTGGTTACAGATGGTTCTTGCATGATAGATCGCCGCATGGGAATTCATGGCCATCCTTTGGAGATTCAGGCACTGTTTTATTCAGCATTACTTTGTGCACGTGAGATGCTTGCTCCAGAGGAAGCATCAGCGGACCTTGTGCGAGCACTTAACAACCGTCTGGTTGCATTGGCCTTCCATATCAGGGAACATTACTGGATTGATATGAGAAAACTCAATGAAATTTACCGATACAAGACAGAGGAATACTCGTACGATGCTGTTAATAAGTTCAATATTTACCCAGATCAGATTCCGTCGTGGCTGGTGGGATTTATGCCCAGTACTGGCGGGTACTTGATTGGAAACCTGCAGCCTGCTCACATGGACTTTCGTTTCTTTTCTCTTGGAAACTTATGGTCTATTGTAAGTAGTCTCGCAACAGACTTGCAGTCACATGCTATTCTGGATCTTATTGAAGCAAAATGGGATGAATTAGTGGCAGATATGCCGTTTAAAATATGTTACCCTGCTCTTGAAGGCCAGGAATGGCAAATCATCACTGGCAGCGATCCCAAAAACACTCCCTGGTCTTACCATAATGGAGGTTCTTGGCCAACGTTGCTCTGGCAGCTCACGGTGGCATGCATAAAACTTAACAGACCAGAAATTGCAGCGAAGGCTGTCGAGATTGCTGAGAAGCGCATATCTATTGACAACTGGCCTGAATATTACGACACCAAGCGAGCAAGATTTATTGGAAAGCAGGCACAGCTGTATCAAACTTGGTCGGTTGCTGGATACCTTGTAGCAAAGCTCCTCCTTGCCAACCCAAGTGCAGCAAAGAATCTTGTGAATGAAGAGGATTCAGAGCTCATAAATGCGTTCTCATGCATGATTAGTGCCAGTCCAAGAAGGAAACGTGGTTGGAAAAAACAGATTTTAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004564 GO:0004575 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005984 GO:0005985 GO:0005987 GO:0006073 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0015926 GO:0016052 GO:0016787 GO:0016798 GO:0022414 GO:0032501 GO:0032502 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0046352 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0061458 GO:0071704 GO:0090599 GO:0099402 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_100

Protein Analysis

653

Amino Acids

73.07

Weight (kDa)

6.66

Isoelectric Point (pI)

47.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_100	PF12899	173 - 614	5.1e-218	Alkaline and neutral invertase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012452)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22510 AT5G22510
fragaria_vesca	FvH4_3g24390
malus_domestica	MD00G1120100.v1.1 MD02G1255300.v1.1 MD11G1159600.v1.1
prunus_persica	Prupe.2G083900_v2.0.a1 Prupe.6G122600_v2.0.a1 Prupe.6G122600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0043881
rosa_laevigata	RLG00000034221
rosa_multiflora	Rmu_sc0009227.1_g000015 Rmu_sc0010851.1_g000004 Rmu_sc0035638.1_g000001
rosa_roxburghii	Rroxscaffold_1G00037180
rosa_rugosa	Rorug05G0208900
rosa_samantha	Rh5AG293000 Rh5CG328700
rosa_wichuraiana	Rw5G027170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1386
AccB7I	CCANNNNNTGG	3 cut(s)	247, 803, 1087
AccI	GTMKAC	1 cut(s)	330
AccIII	TCCGGA	1 cut(s)	929
AciI	CCGC	7 cut(s)	181, 579, 688, 771, 1063, 1160, 1359
AclI	AACGTT	1 cut(s)	1628
AclWI	GGATC	6 cut(s)	411, 581, 830, 843, 1485, 1574
AcoI	YGGCCR	2 cut(s)	1078, 1622
AcsI	RAATTY	2 cut(s)	1071, 1251
AcuI	CTGAAG	3 cut(s)	321, 695, 843
AcvI	CACGTG	1 cut(s)	1130
AdeI	CACNNNGTG	1 cut(s)	143
AfaI	GTAC	4 cut(s)	604, 1283, 1354, 1364
AgsI	TTSAA	6 cut(s)	255, 397, 781, 1303, 1487, 1547
AhdI	GACNNNNNGTC	1 cut(s)	1459
AhlI	ACTAGT	1 cut(s)	374
AjnI	CCWGG	3 cut(s)	751, 1551, 1595
AjuI	GAANNNNNNNTTGG	2 cut(s)	843, 875
AloI	GAACNNNNNNTCC	2 cut(s)	118, 150
Alw21I	GWGCWC	4 cut(s)	929, 1129, 1177, 1884
Alw26I	GTCTC	1 cut(s)	1448
Alw44I	GTGCAC	1 cut(s)	1125
AlwI	GGATC	6 cut(s)	411, 581, 830, 843, 1485, 1574
AlwNI	CAGNNNCTG	1 cut(s)	1102
Aor13HI	TCCGGA	1 cut(s)	929
AoxI	GGCC	5 cut(s)	1078, 1200, 1549, 1622, 1730
ApaLI	GTGCAC	1 cut(s)	1125
ApeKI	GCWGC	8 cut(s)	455, 458, 854, 1381, 1575, 1639, 1682, 1847
ApoI	RAATTY	2 cut(s)	1071, 1251
Asp700I	GAANNNNTTC	1 cut(s)	18
AspLEI	GCGC	1 cut(s)	1713
AspS9I	GGNCC	3 cut(s)	178, 522, 1162
AsuC2I	CCSGG	1 cut(s)	108
AsuHPI	GGTGA	2 cut(s)	818, 860
AvaII	GGWCC	3 cut(s)	178, 522, 1162
BaeGI	GKGCMC	2 cut(s)	148, 1129
BaeI	ACNNNNGTAYC	2 cut(s)	1769, 1802
BalI	TGGCCA	2 cut(s)	1080, 1624
BamHI	GGATCC	1 cut(s)	835
BanII	GRGCYC	1 cut(s)	1884
BbrPI	CACGTG	1 cut(s)	1130
Bbv12I	GWGCWC	4 cut(s)	929, 1129, 1177, 1884
BbvI	GCAGC	8 cut(s)	442, 470, 841, 1393, 1587, 1651, 1694, 1859
BccI	CCATC	4 cut(s)	797, 998, 1034, 1089
BceAI	ACGGC	1 cut(s)	1504
BciT130I	CCWGG	3 cut(s)	753, 1553, 1597
BciVI	GTATCC	1 cut(s)	1802
BclI	TGATCA	1 cut(s)	616
BcnI	CCSGG	1 cut(s)	108
BcoDI	GTCTC	1 cut(s)	1448
BcuI	ACTAGT	1 cut(s)	374
BfaI	CTAG	3 cut(s)	375, 462, 600
BfmI	CTRYAG	2 cut(s)	456, 1379
BfuAI	ACCTGC	1 cut(s)	1386
BfuI	GTATCC	1 cut(s)	1802
BglII	AGATCT	1 cut(s)	934
BmcAI	AGTACT	1 cut(s)	1354
Bme1390I	CCNGG	4 cut(s)	108, 753, 1553, 1597
Bme18I	GGWCC	3 cut(s)	178, 522, 1162
BmeRI	GACNNNNNGTC	1 cut(s)	1459
BmgT120I	GGNCC	3 cut(s)	178, 522, 1162
BmiI	GGNNCC	3 cut(s)	179, 571, 837
BmrFI	CCNGG	4 cut(s)	108, 753, 1553, 1597
BmrI	ACTGGG	3 cut(s)	62, 869, 1344
BmsI	GCATC	3 cut(s)	1125, 1163, 1276
BmuI	ACTGGG	3 cut(s)	62, 869, 1344
BplI	GAGNNNNNCTC	2 cut(s)	1262, 1294
BpmI	CTGGAG	1 cut(s)	1128
BpuMI	CCSGG	1 cut(s)	108
BsaAI	YACGTR	1 cut(s)	1130
BsaJI	CCNNGG	3 cut(s)	540, 735, 1595
BsaWI	WCCGGW	1 cut(s)	929
BsaXI	ACNNNNNCTCC	2 cut(s)	178, 208
Bse118I	RCCGGY	1 cut(s)	451
Bse1I	ACTGG	9 cut(s)	57, 774, 875, 1229, 1350, 1360, 1576, 1733, 1914
Bse3DI	GCAATG	1 cut(s)	573
BseAI	TCCGGA	1 cut(s)	929
BseBI	CCWGG	3 cut(s)	753, 1553, 1597
BseDI	CCNNGG	3 cut(s)	540, 735, 1595
BseGI	GGATG	2 cut(s)	1081, 1504
BseMI	GCAATG	1 cut(s)	573
BseMII	CTCAG	2 cut(s)	131, 1695
BseNI	ACTGG	9 cut(s)	57, 774, 875, 1229, 1350, 1360, 1576, 1733, 1914
BseRI	GAGGAG	2 cut(s)	524, 1817
BseSI	GKGCMC	2 cut(s)	148, 1129
BseXI	GCAGC	8 cut(s)	442, 470, 841, 1393, 1587, 1651, 1694, 1859
BseYI	CCCAGC	1 cut(s)	723
BsgI	GTGCAG	2 cut(s)	965, 1866
BshFI	GGCC	5 cut(s)	1080, 1202, 1551, 1624, 1732
BsiHKAI	GWGCWC	4 cut(s)	929, 1129, 1177, 1884
BsiSI	CCGG	3 cut(s)	108, 452, 930
BsiWI	CGTACG	1 cut(s)	1281
BslFI	GGGAC	2 cut(s)	191, 441
BsmAI	GTCTC	1 cut(s)	1448
BsmFI	GGGAC	2 cut(s)	191, 441
BsnI	GGCC	5 cut(s)	1080, 1202, 1551, 1624, 1732
Bsp1286I	GDGCHC	5 cut(s)	148, 929, 1129, 1177, 1884
Bsp13I	TCCGGA	1 cut(s)	929
Bsp19I	CCATGG	2 cut(s)	540, 735
BspACI	CCGC	7 cut(s)	181, 579, 688, 771, 1063, 1160, 1359
BspANI	GGCC	5 cut(s)	1080, 1202, 1551, 1624, 1732
BspCNI	CTCAG	2 cut(s)	132, 1696
BspEI	TCCGGA	1 cut(s)	929
BspLI	GGNNCC	3 cut(s)	179, 571, 837
BspMAI	CTGCAG	2 cut(s)	460, 1383
BspMI	ACCTGC	1 cut(s)	1386
BspPI	GGATC	6 cut(s)	411, 581, 830, 843, 1485, 1574
BsrDI	GCAATG	1 cut(s)	573
BsrFI	RCCGGY	1 cut(s)	451
BsrI	ACTGG	9 cut(s)	57, 774, 875, 1229, 1350, 1360, 1576, 1733, 1914
BssAI	RCCGGY	1 cut(s)	451
BssECI	CCNNGG	3 cut(s)	540, 735, 1595
BssT1I	CCWWGG	2 cut(s)	540, 735
Bst2UI	CCWGG	3 cut(s)	753, 1553, 1597
Bst4CI	ACNGT	5 cut(s)	560, 793, 1104, 1187, 1648
Bst6I	CTCTTC	1 cut(s)	1863
BstBAI	YACGTR	1 cut(s)	1130
BstC8I	GCNNGC	8 cut(s)	453, 771, 1140, 1173, 1386, 1654, 1755, 1776
BstDEI	CTNAG	3 cut(s)	140, 492, 1704
BstDSI	CCRYGG	2 cut(s)	540, 735
BstENI	CCTNNNNNAGG	2 cut(s)	665, 756
BstF5I	GGATG	2 cut(s)	1081, 1504
BstHHI	GCGC	1 cut(s)	1713
BstMAI	GTCTC	1 cut(s)	1448
BstMWI	GCNNNNNNNGC	4 cut(s)	1630, 1639, 1688, 1780
BstNI	CCWGG	3 cut(s)	753, 1553, 1597
BstNSI	RCATGY	2 cut(s)	1469, 1656
BstSCI	CCNGG	4 cut(s)	106, 751, 1551, 1595
BstSFI	CTRYAG	2 cut(s)	456, 1379
BstSLI	GKGCMC	2 cut(s)	148, 1129
BstV1I	GCAGC	8 cut(s)	442, 470, 841, 1393, 1587, 1651, 1694, 1859
BstX2I	RGATCY	3 cut(s)	835, 934, 1477
BstYI	RGATCY	3 cut(s)	835, 934, 1477
BsuI	GTATCC	1 cut(s)	1802
BsuRI	GGCC	5 cut(s)	1080, 1202, 1551, 1624, 1732
BtgI	CCRYGG	2 cut(s)	540, 735
BtsCI	GGATG	2 cut(s)	1081, 1504
BtsIMutI	CAGTG	5 cut(s)	137, 789, 948, 1100, 1569
BveI	ACCTGC	1 cut(s)	1386
Cac8I	GCNNGC	8 cut(s)	453, 771, 1140, 1173, 1386, 1654, 1755, 1776
CaiI	CAGNNNCTG	1 cut(s)	1102
CfoI	GCGC	1 cut(s)	1713
Cfr10I	RCCGGY	1 cut(s)	451
Cfr13I	GGNCC	3 cut(s)	178, 522, 1162
Csp6I	GTAC	4 cut(s)	603, 1282, 1353, 1363
CviQI	GTAC	4 cut(s)	603, 1282, 1353, 1363
DdeI	CTNAG	3 cut(s)	140, 492, 1704
DraI	TTTAAA	3 cut(s)	666, 987, 1525
DraIII	CACNNNGTG	1 cut(s)	143
DriI	GACNNNNNGTC	1 cut(s)	1459
EaeI	YGGCCR	2 cut(s)	1078, 1622
Eam1104I	CTCTTC	1 cut(s)	1863
Eam1105I	GACNNNNNGTC	1 cut(s)	1459
EarI	CTCTTC	1 cut(s)	1863
Ecl136II	GAGCTC	1 cut(s)	1882
Eco130I	CCWWGG	2 cut(s)	540, 735
Eco24I	GRGCYC	1 cut(s)	1884
Eco47I	GGWCC	3 cut(s)	178, 522, 1162
Eco53kI	GAGCTC	1 cut(s)	1882
Eco57I	CTGAAG	3 cut(s)	321, 695, 843
Eco72I	CACGTG	1 cut(s)	1130
EcoICRI	GAGCTC	1 cut(s)	1882
EcoNI	CCTNNNNNAGG	2 cut(s)	665, 756
EcoRI	GAATTC	1 cut(s)	1071
EcoRII	CCWGG	3 cut(s)	751, 1551, 1595
EcoT14I	CCWWGG	2 cut(s)	540, 735
EcoT22I	ATGCAT	2 cut(s)	1658, 1904
EcoT38I	GRGCYC	1 cut(s)	1884
ErhI	CCWWGG	2 cut(s)	540, 735
FaqI	GGGAC	2 cut(s)	191, 441
FauI	CCCGC	2 cut(s)	778, 1352
FbaI	TGATCA	1 cut(s)	616
FblI	GTMKAC	1 cut(s)	330
FokI	GGATG	2 cut(s)	1068, 1511
FriOI	GRGCYC	1 cut(s)	1884
FspBI	CTAG	3 cut(s)	375, 462, 600
GlaI	GCGC	1 cut(s)	1712
GsaI	CCCAGC	1 cut(s)	727
GsuI	CTGGAG	1 cut(s)	1128
HaeIII	GGCC	5 cut(s)	1080, 1202, 1551, 1624, 1732
HapII	CCGG	3 cut(s)	108, 452, 930
HhaI	GCGC	1 cut(s)	1713
Hin6I	GCGC	1 cut(s)	1711
HinP1I	GCGC	1 cut(s)	1711
HincII	GTYRAC	2 cut(s)	272, 880
HindII	GTYRAC	2 cut(s)	272, 880
HinfI	GANTC	7 cut(s)	251, 415, 881, 1093, 1321, 1855, 1874
HpaII	CCGG	3 cut(s)	108, 452, 930
HphI	GGTGA	2 cut(s)	818, 860
Hpy166II	GTNNAC	5 cut(s)	137, 272, 331, 880, 1127
Hpy188I	TCNGA	4 cut(s)	13, 340, 1320, 1879
Hpy8I	GTNNAC	5 cut(s)	137, 272, 331, 880, 1127
Hpy99I	CGWCG	1 cut(s)	1330
HpyAV	CCTTC	7 cut(s)	391, 654, 719, 1213, 1541, 1681, 1917
HpyCH4III	ACNGT	5 cut(s)	560, 793, 1104, 1187, 1648
HpyCH4IV	ACGT	4 cut(s)	430, 1129, 1628, 1930
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1630, 1639, 1688, 1780
HpyF3I	CTNAG	3 cut(s)	140, 492, 1704
HpySE526I	ACGT	4 cut(s)	430, 1129, 1628, 1930
HspAI	GCGC	1 cut(s)	1711
Kpn2I	TCCGGA	1 cut(s)	929
KroI	GCCGGC	1 cut(s)	451
KroNI	GCCGGC	1 cut(s)	453
Ksp22I	TGATCA	1 cut(s)	616
LmnI	GCTCC	3 cut(s)	932, 1147, 1830
Lsp1109I	GCAGC	8 cut(s)	442, 470, 841, 1393, 1587, 1651, 1694, 1859
LweI	GCATC	3 cut(s)	1125, 1163, 1276
MaeI	CTAG	3 cut(s)	375, 462, 600
MaeII	ACGT	4 cut(s)	430, 1129, 1628, 1930
MaeIII	GTNAC	6 cut(s)	554, 632, 906, 1033, 1461, 1532
MboII	GAAGA	5 cut(s)	314, 317, 692, 836, 1880
MflI	RGATCY	3 cut(s)	835, 934, 1477
MhlI	GDGCHC	5 cut(s)	148, 929, 1129, 1177, 1884
MlsI	TGGCCA	2 cut(s)	1080, 1624
MluCI	AATT	5 cut(s)	392, 1071, 1251, 1502, 1677
MluNI	TGGCCA	2 cut(s)	1080, 1624
MlyI	GAGTC	1 cut(s)	875
MmeI	TCCRAC	4 cut(s)	228, 547, 1046, 1916
Mox20I	TGGCCA	2 cut(s)	1080, 1624
Mph1103I	ATGCAT	2 cut(s)	1658, 1904
MroI	TCCGGA	1 cut(s)	929
MroNI	GCCGGC	1 cut(s)	451
MroXI	GAANNNNTTC	1 cut(s)	18
MscI	TGGCCA	2 cut(s)	1080, 1624
Msp20I	TGGCCA	2 cut(s)	1080, 1624
MspA1I	CMGCKG	2 cut(s)	1160, 1783
MspI	CCGG	3 cut(s)	108, 452, 930
MspR9I	CCNGG	4 cut(s)	108, 753, 1553, 1597
MvaI	CCWGG	3 cut(s)	753, 1553, 1597
MwoI	GCNNNNNNNGC	4 cut(s)	1630, 1639, 1688, 1780
NaeI	GCCGGC	1 cut(s)	453
NciI	CCSGG	1 cut(s)	108
NcoI	CCATGG	2 cut(s)	540, 735
NgoMIV	GCCGGC	1 cut(s)	451
NlaIV	GGNNCC	3 cut(s)	179, 571, 837
NmuCI	GTSAC	2 cut(s)	632, 1461
NsiI	ATGCAT	2 cut(s)	1658, 1904
NspI	RCATGY	2 cut(s)	1469, 1656
PaeI	GCATGC	1 cut(s)	1656
PdiI	GCCGGC	1 cut(s)	453
PdmI	GAANNNNTTC	1 cut(s)	18
PfeI	GAWTC	6 cut(s)	251, 415, 1093, 1321, 1855, 1874
Pfl23II	CGTACG	1 cut(s)	1281
PflMI	CCANNNNNTGG	3 cut(s)	247, 803, 1087
PleI	GAGTC	1 cut(s)	875
PmaCI	CACGTG	1 cut(s)	1130
PmlI	CACGTG	1 cut(s)	1130
PpsI	GAGTC	1 cut(s)	875
Ppu21I	YACGTR	1 cut(s)	1130
Psp124BI	GAGCTC	1 cut(s)	1884
Psp1406I	AACGTT	1 cut(s)	1628
Psp6I	CCWGG	3 cut(s)	751, 1551, 1595
PspCI	CACGTG	1 cut(s)	1130
PspFI	CCCAGC	1 cut(s)	723
PspGI	CCWGG	3 cut(s)	751, 1551, 1595
PspLI	CGTACG	1 cut(s)	1281
PspN4I	GGNNCC	3 cut(s)	179, 571, 837
PspPI	GGNCC	3 cut(s)	178, 522, 1162
PstI	CTGCAG	2 cut(s)	460, 1383
PstNI	CAGNNNCTG	1 cut(s)	1102
PsuI	RGATCY	3 cut(s)	835, 934, 1477
PvuII	CAGCTG	1 cut(s)	1783
RsaI	GTAC	4 cut(s)	604, 1283, 1354, 1364
RsaNI	GTAC	4 cut(s)	603, 1282, 1353, 1363
SacI	GAGCTC	1 cut(s)	1884
Sau96I	GGNCC	3 cut(s)	178, 522, 1162
ScaI	AGTACT	1 cut(s)	1354
SchI	GAGTC	1 cut(s)	875
ScrFI	CCNGG	4 cut(s)	108, 753, 1553, 1597
SduI	GDGCHC	5 cut(s)	148, 929, 1129, 1177, 1884
SfaNI	GCATC	3 cut(s)	1125, 1163, 1276
SfcI	CTRYAG	2 cut(s)	456, 1379
SinI	GGWCC	3 cut(s)	178, 522, 1162
SpeI	ACTAGT	1 cut(s)	374
SphI	GCATGC	1 cut(s)	1656
Sse9I	AATT	5 cut(s)	392, 1071, 1251, 1502, 1677
SsiI	CCGC	7 cut(s)	181, 579, 688, 771, 1063, 1160, 1359
SspI	AATATT	2 cut(s)	1306, 1739
SspMI	CTAG	3 cut(s)	375, 462, 600
SstI	GAGCTC	1 cut(s)	1884
StyD4I	CCNGG	4 cut(s)	106, 751, 1551, 1595
StyI	CCWWGG	2 cut(s)	540, 735
TaaI	ACNGT	5 cut(s)	560, 793, 1104, 1187, 1648
TaiI	ACGT	4 cut(s)	433, 1132, 1631, 1933
TaqI	TCGA	4 cut(s)	22, 49, 164, 1695
TasI	AATT	5 cut(s)	392, 1071, 1251, 1502, 1677
TatI	WGTACW	1 cut(s)	1352
TauI	GCSGC	2 cut(s)	581, 1065
TfiI	GAWTC	6 cut(s)	251, 415, 1093, 1321, 1855, 1874
TscAI	CASTG	5 cut(s)	144, 796, 948, 1107, 1576
TseFI	GTSAC	2 cut(s)	632, 1461
TseI	GCWGC	8 cut(s)	455, 458, 854, 1381, 1575, 1639, 1682, 1847
Tsp45I	GTSAC	2 cut(s)	632, 1461
TspGWI	ACGGA	2 cut(s)	139, 1314
TspRI	CASTG	5 cut(s)	144, 796, 948, 1107, 1576
Van91I	CCANNNNNTGG	3 cut(s)	247, 803, 1087
VneI	GTGCAC	1 cut(s)	1125
VpaK11BI	GGWCC	3 cut(s)	178, 522, 1162
XagI	CCTNNNNNAGG	2 cut(s)	665, 756
XapI	RAATTY	2 cut(s)	1071, 1251
XceI	RCATGY	2 cut(s)	1469, 1656
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1632
XmiI	GTMKAC	1 cut(s)	330
XmnI	GAANNNNTTC	1 cut(s)	18
XspI	CTAG	3 cut(s)	375, 462, 600
ZrmI	AGTACT	1 cut(s)	1354
Zsp2I	ATGCAT	2 cut(s)	1658, 1904

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.