MD02G1255300.v1.1

Alkaline neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012452
Physical Location & Seq
Forward (+)
30725583 .. 30730868
5286 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1255300.v1.1.491

Sequence Viewer

Length: 1959 bp
ATGGGAACTTTCGAGGCAGTTCTTCAAGTTTTTTGCGGTGCTGTACCGCGCCTTTGTAGTACCGATTCATGTTTTGGCAAATGCAGTCCTGCCATTTCTTCTAAGTACCGCGGAAAATGTACAAAGAGAAGAGTCTCAAGAGATATGCAAATGCAACTACTCAGTTCCGGTATGCAACAAATTCGTACAGGAAATTATCGACTTCATGGGATAAGGAGTGGTTTGTTTGGAAAGATGACTGTAGGTGATTCTTGGATTCTGAGTTGCAAGTGCGAACAAGCTGAAAGTATAAGCGGGTCAACTACAAAAGATGAGAACGGAACATGGTTTGTGGATAGTACAAAGAAATTTAACACAATCAACAATGTGGTGAATTCGCCGAATGGTTTGGGGTTTCAAGATATTCAAGAGTTGAAACAGGAAAAGGAGGGCTTGCCAGCTAATGGAACAAATGGAACAGTTAGAGATGCCTTTCACAAGACTAGTATTGATTCCCTTGAGGATGAAGCGTGGGATTTACTACGTGAATCTATGGTTTATTATTGTGGCAGTCCTGTTGGAACCATCGCGGCAAAGGATCCTACCAGTTCCAACACGTTAAATTACGATCAAGTCTTTATTCGTGATTTCATACCTTCTGGCATAGCTTTCCTATTGAAGGGGGAGTATGATATTGTTCGGAACTTTATCCTTCATACACTTCAGTTGCAGAGCTGGGAGAAAACAATGGATTGTCATAGTCCTGGTCAGGGTTTGATGCCTGCTAGTTTTAAAGTTCGTACTGTTCCTTTGGATGGTGATGAATCTGCAACTGAAGAGGTGCTGGATCCAGACTTTGGAGAGGCAGCAATTGGTCGTGTTGCCCCGGTAGATTCTGGGTTATGGTGGATTATCTTGTTACGTGCTTATGGAAAATGCTCGGGTGATCTCTCAGTTCAGGAGAGAGTTGATGTGCAGACCGGGATTAAGATGATTCTAAGGTTATGTATAGCTGATGGTTTTGATATGTTCCCTACATTATTGGTGACCGATGGTTCTTGTATGATAGATCGACGTATGGGAATTCATGGTCACCCTCTGGAAATTCAGGCTCTTTTCTACTCAGCATTACTTTGTGCACGAGAGATGCTTGCTCCAGAGGATGGATCAGCCGATCTTATTCGAGCACTCAATAATCGTCTGGTAGCTCTGTCATTTCATATCAGAGAGTATTATTGGGTTGATTTGAGAAAATTGAATGAAATCTACCGATACAAGACTGAAGAGTACTCATATGATGCAGTGAATAAGTTCAACATTTACCCAGATCAAATATCTTCTTGGCTGGTGGAATGGATGCCCAACAAAGGTGGCTATCTAATTGGAAATTTGCAACCAGCTCATATGGATTTTCGATTCTTTTCTTTGGGGAACTTGTGGTCTGTAGTGAGCAGTATTGCGACAACAGATCAGTCACATGCTATATTGGATTTGATTGAAACCAAATGGGGAGATTTGGTGGCAGATATGCCATTTAAGATTTGTTATCCTGCTCTTGATGGTCAGGAATGGCAGATCATCACAGGGAGTGATCCTAAGAACACGCCGTGGTCCTACCATAATGGAGGTTCCTGGCCAACTTTGTTATGGCAGCTCACTGTCGCTTGCATAAAGATGGATAGACCTGAGATTGCTGCAAAAGCTGTTGAGATTGCTGAGAAACGGATCTCGCGAGACAAGTGGCCAGAATATTATGATACGAAAAGAGGTAGGTTCGTTGGAAAACAAGCGCGCCTGTTCCAGACCTGGTCAATTGCAGGATACCTTGTGGCAAAGCTCTTACTCGCTGATCCTAGCAAAGCCAAGATTTTGATAACTGAAGAGGATTCTGAACTCGTGAATGCCTTCTCCTGCATGATTGGTGCTAACCCAAGAAGAAAGCGTGGTCGAAAGAATTTGAAGCAGACCTACATTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

653

Amino Acids

73.39

Weight (kDa)

6.26

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_100 PF12899 170 - 611 3.5e-221 Alkaline and neutral invertase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 443, 836, 1142
AccII CGCG 5 cut(s) 49, 111, 569, 1711, 1771
AciI CCGC 6 cut(s) 36, 47, 109, 111, 294, 569
AclWI GGATC 8 cut(s) 572, 585, 821, 834, 1153, 1565, 1712, 1823
AcoI YGGCCR 2 cut(s) 1613, 1721
AcsI RAATTY 7 cut(s) 180, 347, 373, 1062, 1083, 1366, 1933
AcuI CTGAAG 4 cut(s) 686, 834, 1281, 1878
AdeI CACNNNGTG 1 cut(s) 1587
AfaI GTAC 8 cut(s) 45, 61, 107, 121, 187, 340, 781, 1268
AfiI CCNNNNNNNGG 7 cut(s) 443, 658, 749, 794, 836, 1142, 1346
AflIII ACRYGT 1 cut(s) 594
AgsI TTSAA 9 cut(s) 26, 398, 407, 415, 658, 1237, 1294, 1478, 1939
AhlI ACTAGT 1 cut(s) 482
AjnI CCWGG 3 cut(s) 742, 1610, 1784
Alw21I GWGCWC 2 cut(s) 1120, 1168
Alw26I GTCTC 2 cut(s) 139, 1707
Alw44I GTGCAC 1 cut(s) 1116
AlwI GGATC 8 cut(s) 572, 585, 821, 834, 1153, 1565, 1712, 1823
Ama87I CYCGRG 1 cut(s) 919
AoxI GGCC 2 cut(s) 1613, 1721
ApaLI GTGCAC 1 cut(s) 1116
ApeKI GCWGC 3 cut(s) 845, 1630, 1673
ApoI RAATTY 7 cut(s) 180, 347, 373, 1062, 1083, 1366, 1933
Asp700I GAANNNNTTC 2 cut(s) 1289, 1883
AspLEI GCGC 3 cut(s) 51, 1771, 1773
AspS9I GGNCC 1 cut(s) 1590
AsuC2I CCSGG 2 cut(s) 866, 961
AsuHPI GGTGA 6 cut(s) 257, 382, 809, 935, 1036, 1064
AvaI CYCGRG 1 cut(s) 919
AvaII GGWCC 1 cut(s) 1590
BaeGI GKGCMC 1 cut(s) 1120
BalI TGGCCA 2 cut(s) 1615, 1723
BamHI GGATCC 2 cut(s) 577, 826
BarI GAAGNNNNNNTAC 1 cut(s) 1931
BauI CACGAG 2 cut(s) 1119, 1874
Bbv12I GWGCWC 2 cut(s) 1120, 1168
BbvI GCAGC 3 cut(s) 857, 1642, 1660
BccI CCATC 7 cut(s) 572, 788, 989, 1025, 1136, 1532, 1648
BceAI ACGGC 1 cut(s) 1570
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BciT130I CCWGG 3 cut(s) 744, 1612, 1786
BciVI GTATCC 1 cut(s) 1793
BcnI CCSGG 2 cut(s) 866, 961
BcoDI GTCTC 2 cut(s) 139, 1707
BcuI ACTAGT 1 cut(s) 482
BfaI CTAG 3 cut(s) 483, 765, 1833
BfmI CTRYAG 2 cut(s) 240, 1422
BfuI GTATCC 1 cut(s) 1793
BisI GCNGC 4 cut(s) 570, 846, 1631, 1674
BlsI GCNGC 4 cut(s) 571, 847, 1632, 1675
BmcAI AGTACT 1 cut(s) 1268
Bme1390I CCNGG 5 cut(s) 744, 866, 961, 1612, 1786
Bme18I GGWCC 1 cut(s) 1590
BmeT110I CYCGRG 1 cut(s) 919
BmgT120I GGNCC 1 cut(s) 1590
BmiI GGNNCC 4 cut(s) 562, 579, 828, 1609
BmrFI CCNGG 5 cut(s) 744, 866, 961, 1612, 1786
BmsI GCATC 5 cut(s) 457, 747, 1116, 1267, 1326
BpmI CTGGAG 1 cut(s) 1119
BpuEI CTTGAG 2 cut(s) 121, 518
BpuMI CCSGG 2 cut(s) 866, 961
BsaAI YACGTR 2 cut(s) 524, 902
BsaJI CCNNGG 3 cut(s) 109, 864, 1586
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 2 cut(s) 1482, 1512
Bsc4I CCNNNNNNNGG 7 cut(s) 443, 658, 749, 794, 836, 1142, 1346
Bse1I ACTGG 1 cut(s) 585
BseBI CCWGG 3 cut(s) 744, 1612, 1786
BseDI CCNNGG 3 cut(s) 109, 864, 1586
BseGI GGATG 4 cut(s) 508, 799, 1147, 1341
BseLI CCNNNNNNNGG 7 cut(s) 443, 658, 749, 794, 836, 1142, 1346
BseMII CTCAG 6 cut(s) 175, 251, 945, 1116, 1656, 1686
BseNI ACTGG 1 cut(s) 585
BsePI GCGCGC 1 cut(s) 1769
BseSI GKGCMC 1 cut(s) 1120
BseXI GCAGC 3 cut(s) 857, 1642, 1660
BseYI CCCAGC 1 cut(s) 714
BsgI GTGCAG 1 cut(s) 974
Bsh1236I CGCG 5 cut(s) 49, 111, 569, 1711, 1771
BshFI GGCC 2 cut(s) 1615, 1723
BsiHKAI GWGCWC 2 cut(s) 1120, 1168
BsiHKCI CYCGRG 1 cut(s) 919
BsiSI CCGG 3 cut(s) 168, 866, 960
BslI CCNNNNNNNGG 7 cut(s) 443, 658, 749, 794, 836, 1142, 1346
BsmAI GTCTC 2 cut(s) 139, 1707
BsmI GAATGC 1 cut(s) 1885
BsnI GGCC 2 cut(s) 1615, 1723
BsoBI CYCGRG 1 cut(s) 919
Bsp1286I GDGCHC 2 cut(s) 1120, 1168
Bsp1407I TGTACA 1 cut(s) 119
Bsp68I TCGCGA 1 cut(s) 1711
BspACI CCGC 6 cut(s) 36, 47, 109, 111, 294, 569
BspANI GGCC 2 cut(s) 1615, 1723
BspCNI CTCAG 6 cut(s) 174, 252, 944, 1115, 1657, 1687
BspFNI CGCG 5 cut(s) 49, 111, 569, 1711, 1771
BspLI GGNNCC 4 cut(s) 562, 579, 828, 1609
BspPI GGATC 8 cut(s) 572, 585, 821, 834, 1153, 1565, 1712, 1823
BsrGI TGTACA 1 cut(s) 119
BsrI ACTGG 1 cut(s) 585
BssECI CCNNGG 3 cut(s) 109, 864, 1586
BssHII GCGCGC 1 cut(s) 1769
BssSI CACGAG 2 cut(s) 1119, 1874
Bst2BI CACGAG 2 cut(s) 1119, 1874
Bst2UI CCWGG 3 cut(s) 744, 1612, 1786
Bst4CI ACNGT 4 cut(s) 241, 460, 784, 1639
Bst6I CTCTTC 4 cut(s) 124, 810, 1257, 1854
BstAUI TGTACA 1 cut(s) 119
BstBAI YACGTR 2 cut(s) 524, 902
BstC8I GCNNGC 6 cut(s) 434, 438, 762, 1131, 1645, 1771
BstDEI CTNAG 9 cut(s) 102, 161, 260, 931, 977, 1102, 1575, 1665, 1695
BstDSI CCRYGG 2 cut(s) 109, 1586
BstEII GGTNACC 2 cut(s) 1024, 1070
BstENI CCTNNNNNAGG 1 cut(s) 656
BstF5I GGATG 4 cut(s) 508, 799, 1147, 1341
BstFNI CGCG 5 cut(s) 49, 111, 569, 1711, 1771
BstHHI GCGC 3 cut(s) 51, 1771, 1773
BstMAI GTCTC 2 cut(s) 139, 1707
BstMWI GCNNNNNNNGC 1 cut(s) 1679
BstNI CCWGG 3 cut(s) 744, 1612, 1786
BstNSI RCATGY 1 cut(s) 1460
BstPI GGTNACC 2 cut(s) 1024, 1070
BstSCI CCNGG 5 cut(s) 742, 864, 959, 1610, 1784
BstSFI CTRYAG 2 cut(s) 240, 1422
BstSLI GKGCMC 1 cut(s) 1120
BstUI CGCG 5 cut(s) 49, 111, 569, 1711, 1771
BstV1I GCAGC 3 cut(s) 857, 1642, 1660
BstX2I RGATCY 3 cut(s) 577, 826, 1704
BstYI RGATCY 3 cut(s) 577, 826, 1704
BsuI GTATCC 1 cut(s) 1793
BsuRI GGCC 2 cut(s) 1615, 1723
BtgI CCRYGG 2 cut(s) 109, 1586
BtgZI GCGATG 1 cut(s) 550
BtsCI GGATG 4 cut(s) 508, 799, 1147, 1341
BtsI GCAGTG 1 cut(s) 1287
BtsIMutI CAGTG 2 cut(s) 1287, 1635
BtuMI TCGCGA 1 cut(s) 1711
Cac8I GCNNGC 6 cut(s) 434, 438, 762, 1131, 1645, 1771
CfoI GCGC 3 cut(s) 51, 1771, 1773
Cfr13I GGNCC 1 cut(s) 1590
Cfr42I CCGCGG 1 cut(s) 112
CsiI ACCWGGT 1 cut(s) 1784
Csp6I GTAC 8 cut(s) 44, 60, 106, 120, 186, 339, 780, 1267
CspCI CAANNNNNGTGG 2 cut(s) 1330, 1365
CviAII CATG 6 cut(s) 69, 206, 324, 1067, 1457, 1894
CviQI GTAC 8 cut(s) 44, 60, 106, 120, 186, 339, 780, 1267
DdeI CTNAG 9 cut(s) 102, 161, 260, 931, 977, 1102, 1575, 1665, 1695
DraI TTTAAA 1 cut(s) 772
DraIII CACNNNGTG 1 cut(s) 1587
EaeI YGGCCR 2 cut(s) 1613, 1721
Eam1104I CTCTTC 4 cut(s) 124, 810, 1257, 1854
EarI CTCTTC 4 cut(s) 124, 810, 1257, 1854
Eco47I GGWCC 1 cut(s) 1590
Eco57I CTGAAG 4 cut(s) 686, 834, 1281, 1878
Eco88I CYCGRG 1 cut(s) 919
Eco91I GGTNACC 2 cut(s) 1024, 1070
EcoNI CCTNNNNNAGG 1 cut(s) 656
EcoO65I GGTNACC 2 cut(s) 1024, 1070
EcoRI GAATTC 2 cut(s) 373, 1062
EcoRII CCWGG 3 cut(s) 742, 1610, 1784
FaeI CATG 6 cut(s) 72, 209, 327, 1070, 1460, 1897
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FatI CATG 6 cut(s) 68, 205, 323, 1066, 1456, 1893
FauI CCCGC 1 cut(s) 287
FauNDI CATATG 2 cut(s) 1273, 1383
Fnu4HI GCNGC 4 cut(s) 570, 846, 1631, 1674
FokI GGATG 4 cut(s) 515, 806, 1154, 1348
Fsp4HI GCNGC 4 cut(s) 570, 846, 1631, 1674
FspBI CTAG 3 cut(s) 483, 765, 1833
GlaI GCGC 3 cut(s) 50, 1770, 1772
GluI GCNGC 4 cut(s) 570, 846, 1631, 1674
GsaI CCCAGC 1 cut(s) 718
GsuI CTGGAG 1 cut(s) 1119
HaeIII GGCC 2 cut(s) 1615, 1723
HapII CCGG 3 cut(s) 168, 866, 960
HhaI GCGC 3 cut(s) 51, 1771, 1773
Hin1II CATG 6 cut(s) 72, 209, 327, 1070, 1460, 1897
Hin6I GCGC 3 cut(s) 49, 1769, 1771
HinP1I GCGC 3 cut(s) 49, 1769, 1771
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HpaII CCGG 3 cut(s) 168, 866, 960
HphI GGTGA 6 cut(s) 257, 382, 809, 935, 1036, 1064
Hpy166II GTNNAC 2 cut(s) 300, 1118
Hpy188I TCNGA 4 cut(s) 261, 681, 1205, 1870
Hpy8I GTNNAC 2 cut(s) 300, 1118
Hpy99I CGWCG 1 cut(s) 1056
HpyAV CCTTC 4 cut(s) 645, 652, 701, 1894
HpyCH4III ACNGT 4 cut(s) 241, 460, 784, 1639
HpyCH4IV ACGT 4 cut(s) 523, 596, 901, 1054
HpyF10VI GCNNNNNNNGC 1 cut(s) 1679
HpyF3I CTNAG 9 cut(s) 102, 161, 260, 931, 977, 1102, 1575, 1665, 1695
HpySE526I ACGT 4 cut(s) 523, 596, 901, 1054
Hsp92II CATG 6 cut(s) 72, 209, 327, 1070, 1460, 1897
HspAI GCGC 3 cut(s) 49, 1769, 1771
KspI CCGCGG 1 cut(s) 112
LmnI GCTCC 1 cut(s) 1138
Lsp1109I GCAGC 3 cut(s) 857, 1642, 1660
LweI GCATC 5 cut(s) 457, 747, 1116, 1267, 1326
MabI ACCWGGT 1 cut(s) 1784
MaeI CTAG 3 cut(s) 483, 765, 1833
MaeII ACGT 4 cut(s) 523, 596, 901, 1054
MaeIII GTNAC 4 cut(s) 897, 1024, 1070, 1452
MboII GAAGA 8 cut(s) 14, 90, 141, 827, 1274, 1308, 1871, 1926
MfeI CAATTG 2 cut(s) 849, 1791
MflI RGATCY 3 cut(s) 577, 826, 1704
MhlI GDGCHC 2 cut(s) 1120, 1168
MlsI TGGCCA 2 cut(s) 1615, 1723
MluNI TGGCCA 2 cut(s) 1615, 1723
MlyI GAGTC 1 cut(s) 141
MmeI TCCRAC 3 cut(s) 538, 615, 1738
Mox20I TGGCCA 2 cut(s) 1615, 1723
MroXI GAANNNNTTC 2 cut(s) 1289, 1883
MscI TGGCCA 2 cut(s) 1615, 1723
MseI TTAA 5 cut(s) 351, 599, 771, 966, 1515
MslI CAYNNNNRTG 1 cut(s) 1652
Msp20I TGGCCA 2 cut(s) 1615, 1723
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 3 cut(s) 168, 866, 960
MspR9I CCNGG 5 cut(s) 744, 866, 961, 1612, 1786
MunI CAATTG 2 cut(s) 849, 1791
Mva1269I GAATGC 1 cut(s) 1885
MvaI CCWGG 3 cut(s) 744, 1612, 1786
MvnI CGCG 5 cut(s) 49, 111, 569, 1711, 1771
MwoI GCNNNNNNNGC 1 cut(s) 1679
NciI CCSGG 2 cut(s) 866, 961
NdeI CATATG 2 cut(s) 1273, 1383
NlaIII CATG 6 cut(s) 72, 209, 327, 1070, 1460, 1897
NlaIV GGNNCC 4 cut(s) 562, 579, 828, 1609
NmuCI GTSAC 3 cut(s) 1024, 1070, 1452
NruI TCGCGA 1 cut(s) 1711
NspI RCATGY 1 cut(s) 1460
PauI GCGCGC 1 cut(s) 1769
PcsI WCGNNNNNNNCGW 1 cut(s) 1708
PctI GAATGC 1 cut(s) 1885
PdmI GAANNNNTTC 2 cut(s) 1289, 1883
PflFI GACNNNGTC 1 cut(s) 1786
PflMI CCANNNNNTGG 3 cut(s) 443, 836, 1142
PkrI GCNGC 4 cut(s) 571, 847, 1632, 1675
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
Ppu21I YACGTR 2 cut(s) 524, 902
Psp6I CCWGG 3 cut(s) 742, 1610, 1784
PspEI GGTNACC 2 cut(s) 1024, 1070
PspFI CCCAGC 1 cut(s) 714
PspGI CCWGG 3 cut(s) 742, 1610, 1784
PspN4I GGNNCC 4 cut(s) 562, 579, 828, 1609
PspPI GGNCC 1 cut(s) 1590
PsuI RGATCY 3 cut(s) 577, 826, 1704
PsyI GACNNNGTC 1 cut(s) 1786
PteI GCGCGC 1 cut(s) 1769
RruI TCGCGA 1 cut(s) 1711
RsaI GTAC 8 cut(s) 45, 61, 107, 121, 187, 340, 781, 1268
RsaNI GTAC 8 cut(s) 44, 60, 106, 120, 186, 339, 780, 1267
RseI CAYNNNNRTG 1 cut(s) 1652
SacII CCGCGG 1 cut(s) 112
SaqAI TTAA 5 cut(s) 351, 599, 771, 966, 1515
SatI GCNGC 4 cut(s) 570, 846, 1631, 1674
Sau96I GGNCC 1 cut(s) 1590
ScaI AGTACT 1 cut(s) 1268
SchI GAGTC 1 cut(s) 141
ScrFI CCNGG 5 cut(s) 744, 866, 961, 1612, 1786
SduI GDGCHC 2 cut(s) 1120, 1168
SexAI ACCWGGT 1 cut(s) 1784
SfaNI GCATC 5 cut(s) 457, 747, 1116, 1267, 1326
SfcI CTRYAG 2 cut(s) 240, 1422
Sfr303I CCGCGG 1 cut(s) 112
SgrBI CCGCGG 1 cut(s) 112
SinI GGWCC 1 cut(s) 1590
SmiMI CAYNNNNRTG 1 cut(s) 1652
SmlI CTYRAG 2 cut(s) 136, 497
SmoI CTYRAG 2 cut(s) 136, 497
SpeI ACTAGT 1 cut(s) 482
SsiI CCGC 6 cut(s) 36, 47, 109, 111, 294, 569
SspI AATATT 1 cut(s) 1730
SspMI CTAG 3 cut(s) 483, 765, 1833
StyD4I CCNGG 5 cut(s) 742, 864, 959, 1610, 1784
TaaI ACNGT 4 cut(s) 241, 460, 784, 1639
TaiI ACGT 4 cut(s) 526, 599, 904, 1057
TaqI TCGA 6 cut(s) 12, 199, 1051, 1162, 1393, 1927
TaqII GACCGA 1 cut(s) 1043
TatI WGTACW 3 cut(s) 119, 338, 1266
TauI GCSGC 1 cut(s) 572
Tru1I TTAA 5 cut(s) 351, 599, 771, 966, 1515
Tru9I TTAA 5 cut(s) 351, 599, 771, 966, 1515
TscAI CASTG 2 cut(s) 1287, 1642
TseFI GTSAC 3 cut(s) 1024, 1070, 1452
TseI GCWGC 3 cut(s) 845, 1630, 1673
Tsp45I GTSAC 3 cut(s) 1024, 1070, 1452
TspDTI ATGAA 9 cut(s) 57, 194, 519, 619, 683, 816, 1055, 1187, 1254
TspGWI ACGGA 2 cut(s) 333, 1717
TspRI CASTG 2 cut(s) 1287, 1642
Tth111I GACNNNGTC 1 cut(s) 1786
Van91I CCANNNNNTGG 3 cut(s) 443, 836, 1142
VneI GTGCAC 1 cut(s) 1116
VpaK11BI GGWCC 1 cut(s) 1590
XagI CCTNNNNNAGG 1 cut(s) 656
XapI RAATTY 7 cut(s) 180, 347, 373, 1062, 1083, 1366, 1933
XceI RCATGY 1 cut(s) 1460
XcmI CCANNNNNNNNNTGG 1 cut(s) 1623
XmnI GAANNNNTTC 2 cut(s) 1289, 1883
XspI CTAG 3 cut(s) 483, 765, 1833
ZrmI AGTACT 1 cut(s) 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.