MD00G1165100.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0022409
Physical Location & Seq
Reverse (-)
37601156 .. 37603178
2023 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1165100.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGAGGAAAAGATGCAGATTTTGGTGCCAATTTTGCATCCTTCGCAAGTGACCACTATTGATGTTAGCCAGCTGCACCAGGACTCTCATACAAGGTCGGTATTGGTCAAAAGTATTCACCATGCTTGCCAAGAAATGGGGTTTTTTCAGGTTATCAATCATGGAATAAGCAAAACAGTCACTGAAAATGCTCTTGGCTCGTTGTCAAACTTCTTTGATTTGCCAATGGATCAGAAGAAGCTATTCTTGTCTGATGATATAAGCAAGCCAGTAAGATTTGTGACTAATTCGAGGGACTCAATCAAACTTTATGCCAACCCTATTGAAAATTGGATTGATTTATGGCCTCATAATCCCACAGAATTCAGAGAAAGTTTGGGAAGATATGCAGCGGAAGTAAAGAGATTAAGCATCGATATACTTGGAGCCATAGTAGAGAGCTTAGGCCTCAGTCCAACTTACTTGAGGAGCAGCCTGGGGCAAGGAATGCAAATGATAGTAGGCAGCCGATATCCTAGGTGCTCCTCATCTAGCAGTATACTCGGAATATCGTCACATACAGACCACAGTATCATCACCATCATTCTTGAAAGTACGTCGGGGGTTGAGATTATGGATTTCACAGACAATAGTGCTTGGAAGTCTGTTCCAGCAACCGACGGTACCCTTAAAGTTCTAGTAGGGAATCATCTCGAAATACTCAGCAACGGGTTGTACAAGAGTGTTTTCCACAGGGTGGTTCCAAACCCTGAAAGGAATAGGGTTTCTATAGGTAGCTTTCTTAGCCTACCCATGGAAGAGATAATGGAGCCTGCCATGGACCTCATTGATGAGCAGCATCCCAAAAGATACAAGGCAAGTAGCTTAGATGAATATATCAAGCTTCTCTCCTCCAAAGAAGAAAAATCCTACATAGAGAGTTTGAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

311

Amino Acids

34.8

Weight (kDa)

5.95

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 19 - 86 4.9e-18 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 165 - 261 6.8e-21 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022409)

Species Orthologous Gene IDs
malus_domestica MD00G1165100.v1.1
prunus_persica Prupe.5G059600_v2.0.a1 Prupe.5G059600_v2.0.a1
pyrus_communis pycom06g04190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 662
AccB1I GGYRCC 2 cut(s) 25, 662
AccB7I CCANNNNNTGG 2 cut(s) 136, 736
AccI GTMKAC 1 cut(s) 538
AciI CCGC 1 cut(s) 392
AclWI GGATC 1 cut(s) 237
AcsI RAATTY 1 cut(s) 362
AdeI CACNNNGTG 1 cut(s) 736
AfaI GTAC 3 cut(s) 595, 664, 716
AfiI CCNNNNNNNGG 3 cut(s) 136, 736, 793
AgsI TTSAA 3 cut(s) 326, 590, 925
AjnI CCWGG 2 cut(s) 78, 474
AjuI GAANNNNNNNTTGG 2 cut(s) 177, 209
AluBI AGCT 6 cut(s) 73, 241, 441, 777, 864, 883
AluI AGCT 6 cut(s) 73, 241, 441, 777, 864, 883
Alw21I GWGCWC 1 cut(s) 524
AlwI GGATC 1 cut(s) 237
AlwNI CAGNNNCTG 1 cut(s) 182
AoxI GGCC 2 cut(s) 344, 445
ApeKI GCWGC 5 cut(s) 73, 389, 471, 504, 835
ApoI RAATTY 1 cut(s) 362
Asp700I GAANNNNTTC 1 cut(s) 242
Asp718I GGTACC 1 cut(s) 662
AspA2I CCTAGG 1 cut(s) 515
AspS9I GGNCC 1 cut(s) 820
AsuHPI GGTGA 2 cut(s) 110, 568
AvaII GGWCC 1 cut(s) 820
AvrII CCTAGG 1 cut(s) 515
BaeI ACNNNNGTAYC 2 cut(s) 553, 586
BanI GGYRCC 2 cut(s) 25, 662
Bbv12I GWGCWC 1 cut(s) 524
BbvI GCAGC 5 cut(s) 60, 401, 483, 516, 847
BccI CCATC 1 cut(s) 587
BcgI CGANNNNNNTGC 2 cut(s) 523, 557
BciT130I CCWGG 2 cut(s) 80, 476
BfaI CTAG 3 cut(s) 516, 531, 677
BfmI CTRYAG 1 cut(s) 768
BisI GCNGC 5 cut(s) 74, 390, 472, 505, 836
BlnI CCTAGG 1 cut(s) 515
BlsI GCNGC 5 cut(s) 75, 391, 473, 506, 837
Bme1390I CCNGG 2 cut(s) 80, 476
Bme18I GGWCC 1 cut(s) 820
BmgT120I GGNCC 1 cut(s) 820
BmiI GGNNCC 5 cut(s) 27, 427, 664, 741, 810
BmrFI CCNGG 2 cut(s) 80, 476
BmsI GCATC 4 cut(s) 3, 46, 420, 847
Bpu10I CCTNAGC 1 cut(s) 442
BpuEI CTTGAG 1 cut(s) 484
Bsa29I ATCGAT 1 cut(s) 414
BsaJI CCNNGG 4 cut(s) 475, 515, 792, 816
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 736, 793
Bse1I ACTGG 1 cut(s) 269
BseBI CCWGG 2 cut(s) 80, 476
BseCI ATCGAT 1 cut(s) 414
BseDI CCNNGG 4 cut(s) 475, 515, 792, 816
BseGI GGATG 2 cut(s) 37, 838
BseLI CCNNNNNNNGG 3 cut(s) 136, 736, 793
BseMII CTCAG 2 cut(s) 463, 715
BseNI ACTGG 1 cut(s) 269
BseRI GAGGAG 3 cut(s) 481, 514, 880
BseXI GCAGC 5 cut(s) 60, 401, 483, 516, 847
BsgI GTGCAG 1 cut(s) 59
BshFI GGCC 2 cut(s) 346, 447
BshNI GGYRCC 2 cut(s) 25, 662
BshVI ATCGAT 1 cut(s) 414
BsiHKAI GWGCWC 1 cut(s) 524
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 3 cut(s) 136, 736, 793
BsmFI GGGAC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 492
BsnI GGCC 2 cut(s) 346, 447
Bsp1286I GDGCHC 1 cut(s) 524
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 1 cut(s) 229
Bsp19I CCATGG 2 cut(s) 792, 816
BspACI CCGC 1 cut(s) 392
BspANI GGCC 2 cut(s) 346, 447
BspCNI CTCAG 2 cut(s) 462, 714
BspDI ATCGAT 1 cut(s) 414
BspLI GGNNCC 5 cut(s) 27, 427, 664, 741, 810
BspPI GGATC 1 cut(s) 237
BspT107I GGYRCC 2 cut(s) 25, 662
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 1 cut(s) 269
BssECI CCNNGG 4 cut(s) 475, 515, 792, 816
BssMI GATC 1 cut(s) 229
BssNAI GTATAC 1 cut(s) 539
BssT1I CCWWGG 3 cut(s) 515, 792, 816
Bst1107I GTATAC 1 cut(s) 539
Bst2UI CCWGG 2 cut(s) 80, 476
Bst4CI ACNGT 3 cut(s) 178, 569, 662
Bst6I CTCTTC 1 cut(s) 792
BstAPI GCANNNNNTGC 1 cut(s) 487
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 4 cut(s) 71, 127, 266, 813
BstDEI CTNAG 5 cut(s) 442, 449, 701, 782, 865
BstDSI CCRYGG 2 cut(s) 792, 816
BstF5I GGATG 2 cut(s) 37, 838
BstKTI GATC 1 cut(s) 232
BstMBI GATC 1 cut(s) 229
BstMWI GCNNNNNNNGC 4 cut(s) 34, 43, 487, 783
BstNI CCWGG 2 cut(s) 80, 476
BstSCI CCNGG 2 cut(s) 78, 474
BstSFI CTRYAG 1 cut(s) 768
BstV1I GCAGC 5 cut(s) 60, 401, 483, 516, 847
BstZ17I GTATAC 1 cut(s) 539
Bsu15I ATCGAT 1 cut(s) 414
BsuRI GGCC 2 cut(s) 346, 447
BsuTUI ATCGAT 1 cut(s) 414
BtgI CCRYGG 2 cut(s) 792, 816
BtsCI GGATG 2 cut(s) 37, 838
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 4 cut(s) 71, 127, 266, 813
CaiI CAGNNNCTG 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 820
ClaI ATCGAT 1 cut(s) 414
Csp6I GTAC 3 cut(s) 594, 663, 715
CviAII CATG 4 cut(s) 122, 161, 793, 817
CviQI GTAC 3 cut(s) 594, 663, 715
DdeI CTNAG 5 cut(s) 442, 449, 701, 782, 865
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
DraIII CACNNNGTG 1 cut(s) 736
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Eco130I CCWWGG 3 cut(s) 515, 792, 816
Eco147I AGGCCT 1 cut(s) 447
Eco32I GATATC 1 cut(s) 512
Eco47I GGWCC 1 cut(s) 820
EcoRI GAATTC 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 78, 474
EcoRV GATATC 1 cut(s) 512
EcoT14I CCWWGG 3 cut(s) 515, 792, 816
ErhI CCWWGG 3 cut(s) 515, 792, 816
FaeI CATG 4 cut(s) 125, 164, 796, 820
FalI AAGNNNNNCTT 2 cut(s) 230, 262
FaqI GGGAC 1 cut(s) 308
FatI CATG 4 cut(s) 121, 160, 792, 816
FblI GTMKAC 1 cut(s) 538
Fnu4HI GCNGC 5 cut(s) 74, 390, 472, 505, 836
FokI GGATG 2 cut(s) 24, 825
Fsp4HI GCNGC 5 cut(s) 74, 390, 472, 505, 836
FspBI CTAG 3 cut(s) 516, 531, 677
GluI GCNGC 5 cut(s) 74, 390, 472, 505, 836
HaeIII GGCC 2 cut(s) 346, 447
Hin1II CATG 4 cut(s) 125, 164, 796, 820
HindIII AAGCTT 1 cut(s) 881
HinfI GANTC 3 cut(s) 83, 296, 685
HphI GGTGA 2 cut(s) 110, 568
Hpy166II GTNNAC 1 cut(s) 539
Hpy188I TCNGA 4 cut(s) 234, 253, 368, 545
Hpy188III TCNNGA 2 cut(s) 587, 692
Hpy8I GTNNAC 1 cut(s) 539
Hpy99I CGWCG 2 cut(s) 601, 662
HpyAV CCTTC 1 cut(s) 51
HpyCH4III ACNGT 3 cut(s) 178, 569, 662
HpyCH4IV ACGT 1 cut(s) 596
HpyCH4V TGCA 5 cut(s) 16, 37, 76, 389, 490
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 43, 487, 783
HpyF3I CTNAG 5 cut(s) 442, 449, 701, 782, 865
HpySE526I ACGT 1 cut(s) 596
Hsp92II CATG 4 cut(s) 125, 164, 796, 820
KpnI GGTACC 1 cut(s) 666
Kzo9I GATC 1 cut(s) 229
LmnI GCTCC 4 cut(s) 425, 468, 527, 808
Lsp1109I GCAGC 5 cut(s) 60, 401, 483, 516, 847
LweI GCATC 4 cut(s) 3, 46, 420, 847
MaeI CTAG 3 cut(s) 516, 531, 677
MaeII ACGT 1 cut(s) 596
MaeIII GTNAC 4 cut(s) 49, 178, 280, 552
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 4 cut(s) 247, 393, 809, 911
MhlI GDGCHC 1 cut(s) 524
MluCI AATT 4 cut(s) 30, 286, 328, 362
MlyI GAGTC 2 cut(s) 77, 290
MmeI TCCRAC 1 cut(s) 479
MnlI CCTC 7 cut(s) 285, 357, 458, 459, 535, 833, 901
MroXI GAANNNNTTC 1 cut(s) 242
MseI TTAA 2 cut(s) 407, 669
MspA1I CMGCKG 2 cut(s) 73, 392
MspR9I CCNGG 2 cut(s) 80, 476
Mva1269I GAATGC 1 cut(s) 492
MvaI CCWGG 2 cut(s) 80, 476
MwoI GCNNNNNNNGC 4 cut(s) 34, 43, 487, 783
NcoI CCATGG 2 cut(s) 792, 816
NdeII GATC 1 cut(s) 229
NlaIII CATG 4 cut(s) 125, 164, 796, 820
NlaIV GGNNCC 5 cut(s) 27, 427, 664, 741, 810
NmuCI GTSAC 4 cut(s) 49, 178, 280, 552
PceI AGGCCT 1 cut(s) 447
PctI GAATGC 1 cut(s) 492
PdmI GAANNNNTTC 1 cut(s) 242
PfeI GAWTC 1 cut(s) 685
PflMI CCANNNNNTGG 2 cut(s) 136, 736
PkrI GCNGC 5 cut(s) 75, 391, 473, 506, 837
PleI GAGTC 2 cut(s) 77, 290
PpsI GAGTC 2 cut(s) 77, 290
Psp6I CCWGG 2 cut(s) 78, 474
PspGI CCWGG 2 cut(s) 78, 474
PspN4I GGNNCC 5 cut(s) 27, 427, 664, 741, 810
PspPI GGNCC 1 cut(s) 820
PstNI CAGNNNCTG 1 cut(s) 182
PvuII CAGCTG 1 cut(s) 73
RsaI GTAC 3 cut(s) 595, 664, 716
RsaNI GTAC 3 cut(s) 594, 663, 715
SaqAI TTAA 2 cut(s) 407, 669
SatI GCNGC 5 cut(s) 74, 390, 472, 505, 836
Sau3AI GATC 1 cut(s) 229
Sau96I GGNCC 1 cut(s) 820
SchI GAGTC 2 cut(s) 77, 290
ScrFI CCNGG 2 cut(s) 80, 476
SduI GDGCHC 1 cut(s) 524
SfaNI GCATC 4 cut(s) 3, 46, 420, 847
SfcI CTRYAG 1 cut(s) 768
SinI GGWCC 1 cut(s) 820
SmlI CTYRAG 1 cut(s) 463
SmoI CTYRAG 1 cut(s) 463
Sse9I AATT 4 cut(s) 30, 286, 328, 362
SseBI AGGCCT 1 cut(s) 447
SsiI CCGC 1 cut(s) 392
SspMI CTAG 3 cut(s) 516, 531, 677
StuI AGGCCT 1 cut(s) 447
StyD4I CCNGG 2 cut(s) 78, 474
StyI CCWWGG 3 cut(s) 515, 792, 816
TaaI ACNGT 3 cut(s) 178, 569, 662
TaiI ACGT 1 cut(s) 599
TaqI TCGA 3 cut(s) 290, 414, 693
TasI AATT 4 cut(s) 30, 286, 328, 362
TatI WGTACW 1 cut(s) 714
TfiI GAWTC 1 cut(s) 685
Tru1I TTAA 2 cut(s) 407, 669
Tru9I TTAA 2 cut(s) 407, 669
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 4 cut(s) 49, 178, 280, 552
TseI GCWGC 5 cut(s) 73, 389, 471, 504, 835
Tsp45I GTSAC 4 cut(s) 49, 178, 280, 552
TspDTI ATGAA 1 cut(s) 885
TspRI CASTG 1 cut(s) 187
Van91I CCANNNNNTGG 2 cut(s) 136, 736
VpaK11BI GGWCC 1 cut(s) 820
XapI RAATTY 1 cut(s) 362
XmaJI CCTAGG 1 cut(s) 515
XmiI GTMKAC 1 cut(s) 538
XmnI GAANNNNTTC 1 cut(s) 242
XspI CTAG 3 cut(s) 516, 531, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.