MD00G1187200.v1.1

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0022021
Physical Location & Seq
Reverse (-)
44366025 .. 44366916
892 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1187200.v1.1.491

Sequence Viewer

Length: 360 bp
ATGAAACTTTCTAGTCTGAGTGCGGCTGCTGCTGCTCTTATGCTTCTTCTTCTTCTTTTACTGGCTGCAAGTTCAGAGGCCGCAATAACATGCAGTGACGTAACCAAGGACCTTAGACCATGCCTGAGCTACCTTGTAAGTGGCTCAGGGAAACCACCTGCTGCATGTTGTGCTGGAGCCTCAGCCCTCGCATCTGCTGTGTCAACCTCTGCTGATAAGAAGGCTGCATGTGCCTGTATTAAATCGGCAGCCCAGAAAATTAATATCAAATCCCAGTTGGCCCAAGCTCTCCCTGGGAACTGTGGGATCAACTTGTCCTTCTCTATTTCTCCAAATACTGACTGTTCAAAGATTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0006869 GO:0008150 GO:0010876 GO:0016020 GO:0033036 GO:0051179 GO:0051234 GO:0071702
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

120

Amino Acids

11.76

Weight (kDa)

8.86

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 22 - 107 4.5e-08 Probable lipid transfer
Tryp_alpha_amyl PF00234 31 - 115 4.5e-12 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18370
malus_domestica MD00G1187200.v1.1
prunus_persica Prupe.2G000900_v2.0.a1
pyrus_communis pycom02g26910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 166
Acc36I ACCTGC 1 cut(s) 166
AciI CCGC 2 cut(s) 23, 81
AclWI GGATC 1 cut(s) 314
AgsI TTSAA 1 cut(s) 348
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 2 cut(s) 129, 287
AluI AGCT 2 cut(s) 129, 287
AlwI GGATC 1 cut(s) 314
AoxI GGCC 2 cut(s) 78, 279
ApeKI GCWGC 7 cut(s) 26, 29, 32, 65, 161, 224, 248
AseI ATTAAT 1 cut(s) 261
AspS9I GGNCC 2 cut(s) 109, 280
AvaII GGWCC 1 cut(s) 109
BbvCI CCTCAGC 1 cut(s) 181
BbvI GCAGC 7 cut(s) 13, 16, 19, 52, 148, 211, 260
BciT130I CCWGG 1 cut(s) 294
BfaI CTAG 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 166
BisI GCNGC 9 cut(s) 24, 27, 30, 33, 66, 81, 162, 225, 249
BlsI GCNGC 9 cut(s) 25, 28, 31, 34, 67, 82, 163, 226, 250
Bme1390I CCNGG 1 cut(s) 294
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 2 cut(s) 109, 280
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 1 cut(s) 294
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 1 cut(s) 200
BmuI ACTGGG 1 cut(s) 268
BpmI CTGGAG 1 cut(s) 195
Bpu10I CCTNAGC 3 cut(s) 125, 145, 181
BsaJI CCNNGG 3 cut(s) 105, 292, 293
Bse1I ACTGG 2 cut(s) 66, 274
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 3 cut(s) 105, 292, 293
BseMII CTCAG 4 cut(s) 8, 116, 159, 195
BseNI ACTGG 2 cut(s) 66, 274
BseXI GCAGC 7 cut(s) 13, 16, 19, 52, 148, 211, 260
BshFI GGCC 2 cut(s) 80, 281
BsnI GGCC 2 cut(s) 80, 281
Bsp143I GATC 1 cut(s) 306
BspACI CCGC 2 cut(s) 23, 81
BspANI GGCC 2 cut(s) 80, 281
BspCNI CTCAG 4 cut(s) 9, 117, 158, 194
BspLI GGNNCC 1 cut(s) 178
BspMI ACCTGC 1 cut(s) 166
BspPI GGATC 1 cut(s) 314
BsrI ACTGG 2 cut(s) 66, 274
BssECI CCNNGG 3 cut(s) 105, 292, 293
BssMI GATC 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 2 cut(s) 302, 344
BstAPI GCANNNNNTGC 1 cut(s) 170
BstDEI CTNAG 5 cut(s) 17, 113, 125, 145, 181
BstKTI GATC 1 cut(s) 309
BstMBI GATC 1 cut(s) 306
BstMWI GCNNNNNNNGC 4 cut(s) 29, 32, 170, 230
BstNI CCWGG 1 cut(s) 294
BstNSI RCATGY 3 cut(s) 93, 168, 231
BstSCI CCNGG 1 cut(s) 292
BstV1I GCAGC 7 cut(s) 13, 16, 19, 52, 148, 211, 260
BsuRI GGCC 2 cut(s) 80, 281
BtsI GCAGTG 1 cut(s) 100
BtsIMutI CAGTG 1 cut(s) 100
BveI ACCTGC 1 cut(s) 166
Cfr13I GGNCC 2 cut(s) 109, 280
CviAII CATG 4 cut(s) 90, 120, 165, 228
DdeI CTNAG 5 cut(s) 17, 113, 125, 145, 181
DpnI GATC 1 cut(s) 308
DpnII GATC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 4 cut(s) 93, 123, 168, 231
FaiI YATR 5 cut(s) 41, 91, 121, 166, 229
FatI CATG 4 cut(s) 89, 119, 164, 227
Fnu4HI GCNGC 9 cut(s) 24, 27, 30, 33, 66, 81, 162, 225, 249
Fsp4HI GCNGC 9 cut(s) 24, 27, 30, 33, 66, 81, 162, 225, 249
FspBI CTAG 1 cut(s) 12
GluI GCNGC 9 cut(s) 24, 27, 30, 33, 66, 81, 162, 225, 249
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 2 cut(s) 80, 281
Hin1II CATG 4 cut(s) 93, 123, 168, 231
HincII GTYRAC 1 cut(s) 204
HindII GTYRAC 1 cut(s) 204
Hpy166II GTNNAC 1 cut(s) 204
Hpy188I TCNGA 2 cut(s) 18, 76
Hpy8I GTNNAC 1 cut(s) 204
HpyAV CCTTC 2 cut(s) 214, 328
HpyCH4III ACNGT 2 cut(s) 302, 344
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 4 cut(s) 68, 93, 164, 227
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 32, 170, 230
HpyF3I CTNAG 5 cut(s) 17, 113, 125, 145, 181
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 4 cut(s) 93, 123, 168, 231
Kzo9I GATC 1 cut(s) 306
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 7 cut(s) 13, 16, 19, 52, 148, 211, 260
LweI GCATC 1 cut(s) 200
MaeI CTAG 1 cut(s) 12
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 95, 100
MalI GATC 1 cut(s) 308
MboI GATC 1 cut(s) 306
MboII GAAGA 3 cut(s) 38, 41, 44
MluCI AATT 1 cut(s) 258
MnlI CCTC 4 cut(s) 70, 190, 197, 217
MseI TTAA 2 cut(s) 240, 261
MspR9I CCNGG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 294
MwoI GCNNNNNNNGC 4 cut(s) 29, 32, 170, 230
NdeII GATC 1 cut(s) 306
NlaIII CATG 4 cut(s) 93, 123, 168, 231
NlaIV GGNNCC 1 cut(s) 178
NmuCI GTSAC 1 cut(s) 95
NspI RCATGY 3 cut(s) 93, 168, 231
PaqCI CACCTGC 1 cut(s) 166
PasI CCCWGGG 1 cut(s) 293
PkrI GCNGC 9 cut(s) 25, 28, 31, 34, 67, 82, 163, 226, 250
PpuMI RGGWCCY 1 cut(s) 109
PshBI ATTAAT 1 cut(s) 261
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 292
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 2 cut(s) 109, 280
PspPPI RGGWCCY 1 cut(s) 109
SaqAI TTAA 2 cut(s) 240, 261
SatI GCNGC 9 cut(s) 24, 27, 30, 33, 66, 81, 162, 225, 249
Sau3AI GATC 1 cut(s) 306
Sau96I GGNCC 2 cut(s) 109, 280
ScrFI CCNGG 1 cut(s) 294
SetI ASST 7 cut(s) 102, 114, 131, 135, 160, 209, 289
SfaNI GCATC 1 cut(s) 200
SinI GGWCC 1 cut(s) 109
Sse9I AATT 1 cut(s) 258
SsiI CCGC 2 cut(s) 23, 81
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 1 cut(s) 292
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 2 cut(s) 302, 344
TaiI ACGT 1 cut(s) 102
TasI AATT 1 cut(s) 258
TauI GCSGC 2 cut(s) 26, 83
Tru1I TTAA 2 cut(s) 240, 261
Tru9I TTAA 2 cut(s) 240, 261
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 7 cut(s) 26, 29, 32, 65, 161, 224, 248
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 109
VspI ATTAAT 1 cut(s) 261
XceI RCATGY 3 cut(s) 93, 168, 231
XcmI CCANNNNNNNNNTGG 1 cut(s) 290
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.