MD01G1006900.v1.1

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0024370
Physical Location & Seq
Forward (+)
2983796 .. 2984113
318 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1006900.v1.1.491

Sequence Viewer

Length: 318 bp
ATGGGTCATATCCACCACATCAACGTGGCTCACTTGGTTGGATTTTGTGCTGATGGATTTGTACGAGCTCTTGTTTACGAGTTATTCCCCAATGGTTCCCTGCAAGATTTCATTTCATCTGCAGATATTAAGAATTCCTTCCTTGGTTGGGATAAGCTGCAAGATATTGCCCTAGGCATAGCCAAAGGAATTGAATATCTTCACCAAGGATGTGATCAACGAATCCTCCATTTCGATATCAAACCCCATAATGTTTTGCTAGACCAAAACTTCACTCCAAAAATTTCTGATTTTGGTTTTGGCCAAGTTATGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

106

Amino Acids

11.78

Weight (kDa)

5.89

Isoelectric Point (pI)

27.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 101 8.8e-17 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 2 - 102 3.5e-19 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024370)

Species Orthologous Gene IDs
malus_domestica MD01G1006900.v1.1 MD02G1249200.v1.1
rosa_chinensis RchiOBHm_Chr3g0482481

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 2 cut(s) 133, 282
AfaI GTAC 1 cut(s) 63
AfiI CCNNNNNNNGG 1 cut(s) 148
AgsI TTSAA 1 cut(s) 194
AluBI AGCT 2 cut(s) 68, 157
AluI AGCT 2 cut(s) 68, 157
Alw21I GWGCWC 1 cut(s) 70
AoxI GGCC 1 cut(s) 301
ApeKI GCWGC 1 cut(s) 157
ApoI RAATTY 2 cut(s) 133, 282
Asp700I GAANNNNTTC 2 cut(s) 137, 198
AspA2I CCTAGG 1 cut(s) 172
AsuHPI GGTGA 1 cut(s) 194
AvrII CCTAGG 1 cut(s) 172
BalI TGGCCA 1 cut(s) 303
BanII GRGCYC 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 144
BccI CCATC 1 cut(s) 47
BclI TGATCA 1 cut(s) 214
BfaI CTAG 2 cut(s) 173, 260
BfmI CTRYAG 1 cut(s) 120
BisI GCNGC 1 cut(s) 158
BlnI CCTAGG 1 cut(s) 172
BlsI GCNGC 1 cut(s) 159
BmiI GGNNCC 1 cut(s) 97
BsaJI CCNNGG 3 cut(s) 142, 172, 205
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 1 cut(s) 148
BseDI CCNNGG 3 cut(s) 142, 172, 205
BseGI GGATG 1 cut(s) 215
BseLI CCNNNNNNNGG 1 cut(s) 148
BseXI GCAGC 1 cut(s) 144
BshFI GGCC 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 70
BslI CCNNNNNNNGG 1 cut(s) 148
BsnI GGCC 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 70
Bsp143I GATC 1 cut(s) 214
BspANI GGCC 1 cut(s) 303
BspLI GGNNCC 1 cut(s) 97
BspMAI CTGCAG 1 cut(s) 124
BssECI CCNNGG 3 cut(s) 142, 172, 205
BssMI GATC 1 cut(s) 214
BssT1I CCWWGG 3 cut(s) 142, 172, 205
BstF5I GGATG 1 cut(s) 215
BstKTI GATC 1 cut(s) 217
BstMBI GATC 1 cut(s) 214
BstSFI CTRYAG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 144
BsuRI GGCC 1 cut(s) 303
BtsCI GGATG 1 cut(s) 215
Csp6I GTAC 1 cut(s) 62
CviJI RGCY 5 cut(s) 29, 68, 157, 182, 303
CviKI_1 RGCY 5 cut(s) 29, 68, 157, 182, 303
CviQI GTAC 1 cut(s) 62
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 301
Ecl136II GAGCTC 1 cut(s) 68
Eco130I CCWWGG 3 cut(s) 142, 172, 205
Eco24I GRGCYC 1 cut(s) 70
Eco32I GATATC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 68
EcoICRI GAGCTC 1 cut(s) 68
EcoRI GAATTC 1 cut(s) 133
EcoRV GATATC 1 cut(s) 238
EcoT14I CCWWGG 3 cut(s) 142, 172, 205
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 3 cut(s) 142, 172, 205
FaiI YATR 4 cut(s) 9, 179, 249, 311
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FbaI TGATCA 1 cut(s) 214
Fnu4HI GCNGC 1 cut(s) 158
FokI GGATG 1 cut(s) 222
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 1 cut(s) 158
FspBI CTAG 2 cut(s) 173, 260
GluI GCNGC 1 cut(s) 158
HaeIII GGCC 1 cut(s) 303
HinfI GANTC 1 cut(s) 222
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 1 cut(s) 289
Hpy8I GTNNAC 1 cut(s) 76
HpyAV CCTTC 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 3 cut(s) 103, 122, 160
HpySE526I ACGT 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 1 cut(s) 214
LpnPI CCDG 1 cut(s) 113
Lsp1109I GCAGC 1 cut(s) 144
MaeI CTAG 2 cut(s) 173, 260
MaeII ACGT 1 cut(s) 24
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MboII GAAGA 1 cut(s) 191
MhlI GDGCHC 1 cut(s) 70
MlsI TGGCCA 1 cut(s) 303
MluCI AATT 3 cut(s) 133, 189, 282
MluNI TGGCCA 1 cut(s) 303
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 1 cut(s) 236
Mox20I TGGCCA 1 cut(s) 303
MroXI GAANNNNTTC 2 cut(s) 137, 198
MscI TGGCCA 1 cut(s) 303
MseI TTAA 1 cut(s) 129
MslI CAYNNNNRTG 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 303
NdeII GATC 1 cut(s) 214
NlaIV GGNNCC 1 cut(s) 97
PdmI GAANNNNTTC 2 cut(s) 137, 198
PfeI GAWTC 1 cut(s) 222
PkrI GCNGC 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 97
PstI CTGCAG 1 cut(s) 124
RsaI GTAC 1 cut(s) 63
RsaNI GTAC 1 cut(s) 62
RseI CAYNNNNRTG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 70
SaqAI TTAA 1 cut(s) 129
SatI GCNGC 1 cut(s) 158
Sau3AI GATC 1 cut(s) 214
SduI GDGCHC 1 cut(s) 70
SetI ASST 3 cut(s) 27, 70, 159
SfcI CTRYAG 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 23
Sse9I AATT 3 cut(s) 133, 189, 282
SspMI CTAG 2 cut(s) 173, 260
SstI GAGCTC 1 cut(s) 70
StyI CCWWGG 3 cut(s) 142, 172, 205
TaiI ACGT 1 cut(s) 27
TaqI TCGA 1 cut(s) 234
TasI AATT 3 cut(s) 133, 189, 282
TfiI GAWTC 1 cut(s) 222
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TseI GCWGC 1 cut(s) 157
TspDTI ATGAA 2 cut(s) 100, 105
XapI RAATTY 2 cut(s) 133, 282
XmaJI CCTAGG 1 cut(s) 172
XmnI GAANNNNTTC 2 cut(s) 137, 198
XspI CTAG 2 cut(s) 173, 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.