MD01G1018100.v1.1
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0024001
Physical Location & Seq
Forward (+)
7826685 .. 7828609
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1018100.v1.1.491

Sequence Viewer

Length: 1470 bp
ATGATTAAGGTTTTTCTGGCTACAATGAACAATCTCACTCTCTCTCTCTACTTCTTCTCCCTTGTAATCCTCTCCGCTTCCTCCGCCTCCGACTCGGAGCTCCGATCCCTTTACGAATTCAAGAAAGGGATCCAAACCGACCCGCTCCGGAAAGTCCTTGACTCATGGACCCTTAGCTCCCTCTCCAACACTCACTCTTGCCCTCCCTGGACTGGCGTTTTCTGCGACACCACCGGCAATGTCGTTGCCTTAGCTCTCGACCATCTTGCCCTCGGCGGCGAGCTCAAGTTCAACACTCTCACTGGACTCACTGCATTGCAAAACCTCACCCTTTCTAACAACGACTTCACGGGTCGGGTCCCGCCGATTCTCGGCACAATGTCATCTCTCCAGCACCTGGATCTATCTGGGAACCGCTTCTACGGCCCGATCCCGGCCCGGATATACGACTTGTGGGGCTTGAATTATCTCAACCTGTCTGCCAATCACTTCAAAGGCGGGTTTCCGGATCGGTTGTGGAATCTCAATCAGCTCAAAGTGTTGGATTTGCATTCCAATCAGCTGTGGGGCGACATTGCTGATTTGTTCTCGCGATTGCACAATGTGGAATACGTTGACTTGAGCAGGAACGAGTTTTTCGGAGGGCTTTCGCTTGCTTCGGAGAATGTTTCGAGCTTATCCAACACAGTGCGGTACTTGAACTTGAGCTACAATAAGCTTGCTGGTGGGTTTTTTAAGTCTGATTCAATTGGGTTGTTTAGGAACTTGCAAGTTTTGGATTTAGGTGGTAACCAGATCACAGGGAAGCTCCCTTCATTTGGGTTGTTGCCGAATTTGCGGGTTTTGAGGCTCGGGAGTAATCAGTTGTTTGGGGAAATACCGGAGGAGTTGTTTGAGAGTTCCATGACAGTGGAGGAATTGGATCTTAGTGGCAATGCTTTGACAGGTTCCATTCATGGAATTAACTCTACAACTTTGAAAGTCTTGAATCTTTCGTCAAATGGTTTGTCTGGCACATTGCAGAATGTGGATATGAGGAGTTGTGTAGTGGTGGATCTCAGTGGAAATAAGATCTCAGGTAACATATCCATTGTGCAGGATTTGGGGGCTGCTTTAGAAGTGCTTGATTTGAGTTCAAATAAGTTTTATGGAAGCTTTCGACAGTTGACATCACAGTTTGAGAAATTAAGTACGCTTAGTCTAAGAGATAATTTATTAGTTGGTCCTTTACCTTCAATCTTGAAGGCCTGTCCTAGACTCTCGACAGTTGACTTGAGCCTAAATGATTTCAGTGGGTCCATTCCTGGAAGTTTCCTCTCTTCAACGACCCTAAAGAGACTGAATCTTTCAGGGAATCATTTAATTGGGCCAATTCCTCTTGAAGGTGCACATGTAAAGGAATTGTTATCTCTACCTCCAGATTTGCCGATTGAGACTGATCTCTCCCATCTTTACGGCGCCGCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

490

Amino Acids

53.2

Weight (kDa)

5.82

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 76 1.8e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 84 - 209 6.1e-06 Leucine-rich repeat region
LRR_4 PF12799 255 - 289 2.3e-07 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024001)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25730
malus_domestica MD01G1018100.v1.1
rosa_samantha Rh5BG123000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1457
AccB7I CCANNNNNTGG 1 cut(s) 397
AccBSI CCGCTC 1 cut(s) 145
AccII CGCG 1 cut(s) 592
AccIII TCCGGA 2 cut(s) 147, 505
AclWI GGATC 8 cut(s) 99, 124, 137, 408, 424, 516, 930, 1062
AcsI RAATTY 2 cut(s) 116, 832
AcyI GRCGYC 1 cut(s) 1458
AdeI CACNNNGTG 1 cut(s) 604
AfaI GTAC 2 cut(s) 695, 1192
AfiI CCNNNNNNNGG 6 cut(s) 212, 371, 397, 433, 818, 1382
AflIII ACRYGT 1 cut(s) 1390
AjnI CCWGG 3 cut(s) 206, 396, 1303
Alw21I GWGCWC 3 cut(s) 102, 285, 1390
Alw26I GTCTC 2 cut(s) 1330, 1427
Alw44I GTGCAC 1 cut(s) 1386
AlwI GGATC 8 cut(s) 99, 124, 137, 408, 424, 516, 930, 1062
AlwNI CAGNNNCTG 1 cut(s) 397
Ama87I CYCGRG 1 cut(s) 851
Aor13HI TCCGGA 2 cut(s) 147, 505
AoxI GGCC 4 cut(s) 424, 435, 1245, 1367
ApaLI GTGCAC 1 cut(s) 1386
ApeKI GCWGC 1 cut(s) 1109
ApoI RAATTY 2 cut(s) 116, 832
ArsI GACNNNNNNTTYG 2 cut(s) 992, 1024
Asp700I GAANNNNTTC 1 cut(s) 416
AspLEI GCGC 1 cut(s) 1460
AspS9I GGNCC 7 cut(s) 168, 358, 425, 436, 1223, 1296, 1367
AsuC2I CCSGG 2 cut(s) 434, 439
AsuHPI GGTGA 1 cut(s) 319
AvaI CYCGRG 1 cut(s) 851
AvaII GGWCC 4 cut(s) 168, 358, 1223, 1296
BaeGI GKGCMC 1 cut(s) 1390
BamHI GGATCC 1 cut(s) 129
BanI GGYRCC 1 cut(s) 1457
BanII GRGCYC 2 cut(s) 102, 285
Bbv12I GWGCWC 3 cut(s) 102, 285, 1390
BbvI GCAGC 1 cut(s) 1096
BccI CCATC 2 cut(s) 270, 1455
BceAI ACGGC 1 cut(s) 439
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 3 cut(s) 208, 398, 1305
BcnI CCSGG 2 cut(s) 434, 439
BcoDI GTCTC 2 cut(s) 1330, 1427
BfaI CTAG 1 cut(s) 1254
BfoI RGCGCY 1 cut(s) 1461
BglII AGATCT 1 cut(s) 1071
BisI GCNGC 3 cut(s) 277, 1110, 1461
BlsI GCNGC 3 cut(s) 278, 1111, 1462
Bme1390I CCNGG 5 cut(s) 208, 398, 434, 439, 1305
Bme18I GGWCC 4 cut(s) 168, 358, 1223, 1296
BmeT110I CYCGRG 1 cut(s) 851
BmgT120I GGNCC 7 cut(s) 168, 358, 425, 436, 1223, 1296, 1367
BmiI GGNNCC 8 cut(s) 131, 170, 359, 360, 413, 949, 1297, 1459
BmrFI CCNGG 5 cut(s) 208, 398, 434, 439, 1305
BpmI CTGGAG 2 cut(s) 374, 1401
Bpu10I CCTNAGC 2 cut(s) 173, 250
BpuEI CTTGAG 4 cut(s) 269, 640, 724, 1294
BpuMI CCSGG 2 cut(s) 434, 439
BsaHI GRCGYC 1 cut(s) 1458
BsaJI CCNNGG 2 cut(s) 206, 271
BsaWI WCCGGW 3 cut(s) 147, 505, 880
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 875, 905
Bsc4I CCNNNNNNNGG 6 cut(s) 212, 371, 397, 433, 818, 1382
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 2 cut(s) 217, 307
Bse3DI GCAATG 5 cut(s) 244, 314, 573, 940, 1016
BseAI TCCGGA 2 cut(s) 147, 505
BseBI CCWGG 3 cut(s) 208, 398, 1305
BseDI CCNNGG 2 cut(s) 206, 271
BseLI CCNNNNNNNGG 6 cut(s) 212, 371, 397, 433, 818, 1382
BseMI GCAATG 5 cut(s) 244, 314, 573, 940, 1016
BseMII CTCAG 2 cut(s) 1072, 1089
BseNI ACTGG 2 cut(s) 217, 307
BseRI GAGGAG 2 cut(s) 899, 1051
BseSI GKGCMC 1 cut(s) 1390
BseXI GCAGC 1 cut(s) 1096
BsgI GTGCAG 1 cut(s) 1115
Bsh1236I CGCG 1 cut(s) 592
BshFI GGCC 4 cut(s) 426, 437, 1247, 1369
BshNI GGYRCC 1 cut(s) 1457
BsiHKAI GWGCWC 3 cut(s) 102, 285, 1390
BsiHKCI CYCGRG 1 cut(s) 851
BsiSI CCGG 6 cut(s) 148, 234, 434, 439, 506, 881
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 6 cut(s) 212, 371, 397, 433, 818, 1382
BsmAI GTCTC 2 cut(s) 1330, 1427
BsmFI GGGAC 1 cut(s) 344
BsmI GAATGC 1 cut(s) 550
BsnI GGCC 4 cut(s) 426, 437, 1247, 1369
BsoBI CYCGRG 1 cut(s) 851
Bsp1286I GDGCHC 3 cut(s) 102, 285, 1390
Bsp13I TCCGGA 2 cut(s) 147, 505
Bsp68I TCGCGA 1 cut(s) 592
BspANI GGCC 4 cut(s) 426, 437, 1247, 1369
BspCNI CTCAG 2 cut(s) 1071, 1088
BspEI TCCGGA 2 cut(s) 147, 505
BspFNI CGCG 1 cut(s) 592
BspLI GGNNCC 8 cut(s) 131, 170, 359, 360, 413, 949, 1297, 1459
BspPI GGATC 8 cut(s) 99, 124, 137, 408, 424, 516, 930, 1062
BspT107I GGYRCC 1 cut(s) 1457
BsrBI CCGCTC 1 cut(s) 145
BsrDI GCAATG 5 cut(s) 244, 314, 573, 940, 1016
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 2 cut(s) 217, 307
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 206, 271
BssNI GRCGYC 1 cut(s) 1458
Bst2UI CCWGG 3 cut(s) 208, 398, 1305
Bst4CI ACNGT 5 cut(s) 688, 910, 1164, 1176, 1267
Bst6I CTCTTC 1 cut(s) 1324
BstACI GRCGYC 1 cut(s) 1458
BstC8I GCNNGC 3 cut(s) 281, 654, 720
BstDEI CTNAG 7 cut(s) 173, 250, 926, 1058, 1075, 1196, 1202
BstEII GGTNACC 1 cut(s) 788
BstENI CCTNNNNNAGG 1 cut(s) 1380
BstFNI CGCG 1 cut(s) 592
BstH2I RGCGCY 1 cut(s) 1461
BstHHI GCGC 1 cut(s) 1460
BstMAI GTCTC 2 cut(s) 1330, 1427
BstMWI GCNNNNNNNGC 4 cut(s) 83, 222, 423, 835
BstNI CCWGG 3 cut(s) 208, 398, 1305
BstNSI RCATGY 1 cut(s) 1394
BstPI GGTNACC 1 cut(s) 788
BstSCI CCNGG 5 cut(s) 206, 396, 432, 437, 1303
BstSLI GKGCMC 1 cut(s) 1390
BstUI CGCG 1 cut(s) 592
BstV1I GCAGC 1 cut(s) 1096
BstX2I RGATCY 5 cut(s) 129, 400, 922, 1054, 1071
BstXI CCANNNNNNTGG 1 cut(s) 910
BstYI RGATCY 5 cut(s) 129, 400, 922, 1054, 1071
BsuRI GGCC 4 cut(s) 426, 437, 1247, 1369
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 6 cut(s) 300, 309, 693, 915, 1066, 1297
BtuMI TCGCGA 1 cut(s) 592
Cac8I GCNNGC 3 cut(s) 281, 654, 720
CaiI CAGNNNCTG 1 cut(s) 397
CfoI GCGC 1 cut(s) 1460
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 7 cut(s) 168, 358, 425, 436, 1223, 1296, 1367
Csp6I GTAC 2 cut(s) 694, 1191
CviAII CATG 4 cut(s) 165, 904, 956, 1391
CviQI GTAC 2 cut(s) 694, 1191
DdeI CTNAG 7 cut(s) 173, 250, 926, 1058, 1075, 1196, 1202
DinI GGCGCC 1 cut(s) 1459
DraIII CACNNNGTG 1 cut(s) 604
Eam1104I CTCTTC 1 cut(s) 1324
EarI CTCTTC 1 cut(s) 1324
EciI GGCGGA 1 cut(s) 73
Ecl136II GAGCTC 2 cut(s) 100, 283
Eco147I AGGCCT 1 cut(s) 1247
Eco24I GRGCYC 2 cut(s) 102, 285
Eco47I GGWCC 4 cut(s) 168, 358, 1223, 1296
Eco53kI GAGCTC 2 cut(s) 100, 283
Eco88I CYCGRG 1 cut(s) 851
Eco91I GGTNACC 1 cut(s) 788
EcoICRI GAGCTC 2 cut(s) 100, 283
EcoNI CCTNNNNNAGG 1 cut(s) 1380
EcoO109I RGGNCCY 1 cut(s) 358
EcoO65I GGTNACC 1 cut(s) 788
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 3 cut(s) 206, 396, 1303
EcoT38I GRGCYC 2 cut(s) 102, 285
EgeI GGCGCC 1 cut(s) 1459
EheI GGCGCC 1 cut(s) 1459
FaeI CATG 4 cut(s) 168, 907, 959, 1394
FaiI YATR 8 cut(s) 166, 445, 905, 957, 1034, 1085, 1149, 1392
FaqI GGGAC 1 cut(s) 344
FatI CATG 4 cut(s) 164, 903, 955, 1390
FauI CCCGC 4 cut(s) 150, 369, 491, 831
Fnu4HI GCNGC 3 cut(s) 277, 1110, 1461
FriOI GRGCYC 2 cut(s) 102, 285
Fsp4HI GCNGC 3 cut(s) 277, 1110, 1461
FspBI CTAG 1 cut(s) 1254
GlaI GCGC 1 cut(s) 1459
GluI GCNGC 3 cut(s) 277, 1110, 1461
GsuI CTGGAG 2 cut(s) 374, 1401
HaeII RGCGCY 1 cut(s) 1461
HaeIII GGCC 4 cut(s) 426, 437, 1247, 1369
HapII CCGG 6 cut(s) 148, 234, 434, 439, 506, 881
HhaI GCGC 1 cut(s) 1460
Hin1I GRCGYC 1 cut(s) 1458
Hin1II CATG 4 cut(s) 168, 907, 959, 1394
Hin6I GCGC 1 cut(s) 1458
HinP1I GCGC 1 cut(s) 1458
HincII GTYRAC 3 cut(s) 616, 1167, 1270
HindII GTYRAC 3 cut(s) 616, 1167, 1270
HindIII AAGCTT 2 cut(s) 716, 1153
HpaII CCGG 6 cut(s) 148, 234, 434, 439, 506, 881
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 4 cut(s) 616, 1167, 1270, 1388
Hpy188I TCNGA 6 cut(s) 91, 97, 104, 641, 661, 742
Hpy8I GTNNAC 4 cut(s) 616, 1167, 1270, 1388
HpyAV CCTTC 4 cut(s) 822, 1237, 1242, 1376
HpyCH4III ACNGT 5 cut(s) 688, 910, 1164, 1176, 1267
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 8 cut(s) 314, 319, 550, 598, 769, 1021, 1096, 1388
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 222, 423, 835
HpyF3I CTNAG 7 cut(s) 173, 250, 926, 1058, 1075, 1196, 1202
HpySE526I ACGT 1 cut(s) 612
Hsp92I GRCGYC 1 cut(s) 1458
Hsp92II CATG 4 cut(s) 168, 907, 959, 1394
HspAI GCGC 1 cut(s) 1458
KasI GGCGCC 1 cut(s) 1457
KflI GGGWCCC 1 cut(s) 358
Kpn2I TCCGGA 2 cut(s) 147, 505
LmnI GCTCC 5 cut(s) 97, 105, 150, 182, 813
Lsp1109I GCAGC 1 cut(s) 1096
MaeI CTAG 1 cut(s) 1254
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 2 cut(s) 788, 1079
MbiI CCGCTC 1 cut(s) 145
MboII GAAGA 2 cut(s) 46, 1311
MfeI CAATTG 1 cut(s) 747
MflI RGATCY 5 cut(s) 129, 400, 922, 1054, 1071
MhlI GDGCHC 3 cut(s) 102, 285, 1390
Mly113I GGCGCC 1 cut(s) 1458
MlyI GAGTC 4 cut(s) 86, 155, 300, 1251
MmeI TCCRAC 4 cut(s) 114, 210, 522, 705
MroI TCCGGA 2 cut(s) 147, 505
MroXI GAANNNNTTC 1 cut(s) 416
MseI TTAA 5 cut(s) 6, 735, 963, 1187, 1361
MslI CAYNNNNRTG 1 cut(s) 908
MspA1I CMGCKG 1 cut(s) 562
MspI CCGG 6 cut(s) 148, 234, 434, 439, 506, 881
MspR9I CCNGG 5 cut(s) 208, 398, 434, 439, 1305
MunI CAATTG 1 cut(s) 747
Mva1269I GAATGC 1 cut(s) 550
MvaI CCWGG 3 cut(s) 208, 398, 1305
MvnI CGCG 1 cut(s) 592
MwoI GCNNNNNNNGC 4 cut(s) 83, 222, 423, 835
NarI GGCGCC 1 cut(s) 1458
NciI CCSGG 2 cut(s) 434, 439
NlaIII CATG 4 cut(s) 168, 907, 959, 1394
NlaIV GGNNCC 8 cut(s) 131, 170, 359, 360, 413, 949, 1297, 1459
NmeAIII GCCGAG 2 cut(s) 252, 351
NruI TCGCGA 1 cut(s) 592
NspI RCATGY 1 cut(s) 1394
PceI AGGCCT 1 cut(s) 1247
PciI ACATGT 1 cut(s) 1390
PctI GAATGC 1 cut(s) 550
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 6 cut(s) 367, 520, 743, 988, 1342, 1354
PflMI CCANNNNNTGG 1 cut(s) 397
PfoI TCCNGGA 1 cut(s) 1303
PkrI GCNGC 3 cut(s) 278, 1111, 1462
PleI GAGTC 4 cut(s) 86, 155, 300, 1251
PluTI GGCGCC 1 cut(s) 1461
PpsI GAGTC 4 cut(s) 86, 155, 300, 1251
PpuMI RGGWCCY 1 cut(s) 358
PscI ACATGT 1 cut(s) 1390
Psp124BI GAGCTC 2 cut(s) 102, 285
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 3 cut(s) 206, 396, 1303
PspEI GGTNACC 1 cut(s) 788
PspGI CCWGG 3 cut(s) 206, 396, 1303
PspN4I GGNNCC 8 cut(s) 131, 170, 359, 360, 413, 949, 1297, 1459
PspPI GGNCC 7 cut(s) 168, 358, 425, 436, 1223, 1296, 1367
PspPPI RGGWCCY 1 cut(s) 358
PsrI GAACNNNNNNTAC 4 cut(s) 404, 436, 692, 724
PstNI CAGNNNCTG 1 cut(s) 397
PsuI RGATCY 5 cut(s) 129, 400, 922, 1054, 1071
PvuII CAGCTG 1 cut(s) 562
RruI TCGCGA 1 cut(s) 592
RsaI GTAC 2 cut(s) 695, 1192
RsaNI GTAC 2 cut(s) 694, 1191
RseI CAYNNNNRTG 1 cut(s) 908
SacI GAGCTC 2 cut(s) 102, 285
SaqAI TTAA 5 cut(s) 6, 735, 963, 1187, 1361
SatI GCNGC 3 cut(s) 277, 1110, 1461
Sau96I GGNCC 7 cut(s) 168, 358, 425, 436, 1223, 1296, 1367
SchI GAGTC 4 cut(s) 86, 155, 300, 1251
ScrFI CCNGG 5 cut(s) 208, 398, 434, 439, 1305
SduI GDGCHC 3 cut(s) 102, 285, 1390
SfoI GGCGCC 1 cut(s) 1459
SinI GGWCC 4 cut(s) 168, 358, 1223, 1296
SmiMI CAYNNNNRTG 1 cut(s) 908
SmlI CTYRAG 4 cut(s) 284, 619, 703, 1273
SmoI CTYRAG 4 cut(s) 284, 619, 703, 1273
SseBI AGGCCT 1 cut(s) 1247
SspDI GGCGCC 1 cut(s) 1457
SspMI CTAG 1 cut(s) 1254
SstI GAGCTC 2 cut(s) 102, 285
StuI AGGCCT 1 cut(s) 1247
StyD4I CCNGG 5 cut(s) 206, 396, 432, 437, 1303
TaaI ACNGT 5 cut(s) 688, 910, 1164, 1176, 1267
TaiI ACGT 1 cut(s) 615
TaqI TCGA 4 cut(s) 258, 671, 1159, 1262
TauI GCSGC 2 cut(s) 279, 1463
TfiI GAWTC 6 cut(s) 367, 520, 743, 988, 1342, 1354
Tru1I TTAA 5 cut(s) 6, 735, 963, 1187, 1361
Tru9I TTAA 5 cut(s) 6, 735, 963, 1187, 1361
TscAI CASTG 6 cut(s) 307, 316, 693, 915, 1066, 1297
TseI GCWGC 1 cut(s) 1109
TspDTI ATGAA 3 cut(s) 41, 804, 944
TspRI CASTG 6 cut(s) 307, 316, 693, 915, 1066, 1297
Van91I CCANNNNNTGG 1 cut(s) 397
VneI GTGCAC 1 cut(s) 1386
VpaK11BI GGWCC 4 cut(s) 168, 358, 1223, 1296
XagI CCTNNNNNAGG 1 cut(s) 1380
XapI RAATTY 2 cut(s) 116, 832
XceI RCATGY 1 cut(s) 1394
XmnI GAANNNNTTC 1 cut(s) 416
XspI CTAG 1 cut(s) 1254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.