Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0015890

Physical Location & Seq

Genomic Coordinates Reverse (-)

9247063 .. 9253939

Gene Structure 6877 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1024600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGGGCTCTTCAGAGCATTCTGAAGAGGCTAATTGGGTTCTCCATACGCCCCGGCTGCCGGGACTATGGCGTGAGTTTACAGAGGCCTCAGGTGAAGAAATTAGTCCCAAAGATACCCTTCCAGCTCCAGGTTCCACCAAACACTTCAAAAAATCAAGCACTGCCTCATCAGCTATTGACGAGGAAAGGCTTGACATCGATCTTGACCTTGAGAAAAACAGCAGCCTAGTTGAGAGGACTCAGTGGGTGCTGAATGGTCCTGAACCCCCTGGTCTATGGCATGAGCTCATGGACTCTCTGAGGGAAACCATTTCTTGTTGCCGAAAAAAGTACTCGTCTCTCAAGAATCAGCCAACACTCAAAAGTGCAGTCTCTGTCCAGCAGGAGATATTTCCGATTCTTGTATGGGGTAGAAGCTACAACATGTCAAAATTCAAACATGATTTAATGGCAGGTCTCACTATAGCAAGTCTCTGCATTCCTCAGAGTATTGGATATGCAACTCTAGCAAAGCTTGACCCTCAGTACGGCCTTTACACAAGTGTTGTACCACCTCTTATTTATGCTGTAATGGGGACTTCAAGAGAGATAGCAATTGGACCTGTGGCTGTTGTTTCTCTTCTTTTGCCCTCAATGATTCAGAAGTTACAGGACCCCGAGGCTGATCCTATTGCTTACACCAAGCTCGTACTCACTGCAACGTTCTTCACCGGTATCTTTCAAGCTGCCTTCGGACTCTTCAGATTGGGGTTTCTTGTGGATTTTCTTTCTCATGCTGCAATCGTAGGGTTCGTGGCCGGGGCAGCCATCATAATCGGTCTTCAACAACTAAAGGGACTACTTGGAATCACTCACTTTCCAACCAATACTGATGTTATCTCTGTTATAGAAGCTGTTTGGACATCTTTCCACCACCCTTGGAGTCCTCATAATTTTTTCCTAGGCTGCTCATTCCTGTGTTTCATCCTAATGTCAAGATTTCTGGGTAAAAAGAAGAAGAAACTTTTCTGGTTGCCAGCCGTTGCTCCTCTCCTATCTGTTATACTATCGACTCTGATTGTTTATCTAACACGGGGAGATAAACATGGAATTAATATTGTTAAACACGTCAAAGATGGCTTGAACCCTAGTTCGGTGAATCTGTTACAGTTCAACAGCCCCTATGTTGGGGCTGTGGCTAAAGTTGGGCTAATTGTCGCCCTTGTTGCACTCGCGGAAGCAATTGCAGTTGGAAGATCTTTCTCAACCATGAAAGGATACCACATAGATGGTAACAAAGAAATGATGGCAATGGGATTCATGAACGTAGTGGGATCCTGTACTTCCTGCTATGTTGCAACCGGTTCATTCTCGCGCACAGCCGTTAACTTCAGTGCTGGTTGTGAAACTCCTGTGTCAAACGTGGTCATGGCCTTTACTGTGATCATATCACTGCAGCTGTTGACAAAGCTCTTGTATTACACACCAACAGCAATCCTTGCTTCAATTATTCTCTCTGCTCTGTCCGGGCTTATTAACCTGAGCGAAGTCTACAAAATTTGGAAGGTTGATAAGTTGGACTTCTTGGCTTGCATTGGAGCCTTCTTTGGGGTCCTATTTGCATCCATGGAGATTGGCCTTCTAGTTGCGGTGATCATATCATTCACAAAGATTATTCTCATCTCAATTGGACCAGGCACAGAAACACTTGGAAAACTTCCTAGAACTGATATGTTTTGCGACACTGCTCAGTATCCTATGGCTGTTAAAATTCCTGGAATCATGATAATACGTGTCAAGTCTGCCTTGTTTTGTTTTGCAAATGCCAATTTCGTCAGAGAAAGGATTGTAAGGTGGATAACTGCAAAGAAAGGGGAGGACACCAAAGAGGCAATTCAACTCGTAATTCTTGACATGTCTAATTTGATTAACATTGATACATCGGGAATAGCTACTCTAGAGGAGCTGCAAAAGAATTTGATCTCCGAAGGAATTGAGTTAGCCGTTGCAAACCCTAGGTGGCTAGTGATTCACAAACTAAAGCTATCCAACTTTGTGGAAAAAATTGGAGGAAGGGTCTTCGTGACCGTCGGAGAAGCAGTGGATGCAAGATTCACTGAAAAATTGCCACCACCTGCTAATGCAAGCTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008272 GO:0008509 GO:0015075 GO:0015103 GO:0015116 GO:0015318 GO:0015698 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0031226 GO:0034220 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0072348 GO:0098656 GO:0098660 GO:0098661 GO:1901682 GO:1902358

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

STAS Sulfate_transp

Protein Analysis

711

Amino Acids

77.56

Weight (kDa)

8.55

Isoelectric Point (pI)

34.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sulfate_transp	PF00916	145 - 525	9.7e-138	Sulfate permease family
STAS	PF01740	578 - 693	3.2e-22	STAS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015890)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10180
fragaria_vesca	FvH4_1g21480
malus_domestica	MD01G1024600.v1.1
prunus_persica	Prupe.6G180000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0115361
rosa_laevigata	RLG00000018179
rosa_multiflora	Rmu_sc0001897.1_g000007
rosa_roxburghii	Rroxscaffold_2G00130850
rosa_rugosa	Rorug02G0195700 Rorug02G0195800
rosa_samantha	Rh2AG254200 Rh2BG264400 Rh2CG258900 Rh2DG262300
rosa_wichuraiana	Rw2G019850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2122
Acc36I	ACCTGC	2 cut(s)	443, 2122
AccI	GTMKAC	1 cut(s)	1530
AccII	CGCG	2 cut(s)	1214, 1354
AciI	CCGC	2 cut(s)	1214, 1628
AclI	AACGTT	1 cut(s)	701
AclWI	GGATC	3 cut(s)	659, 1308, 1321
AcoI	YGGCCR	1 cut(s)	795
AcsI	RAATTY	4 cut(s)	431, 1536, 1749, 1954
AcuI	CTGAAG	3 cut(s)	42, 724, 1354
AfaI	GTAC	5 cut(s)	332, 527, 549, 690, 1321
AfiI	CCNNNNNNNGG	7 cut(s)	58, 128, 527, 1132, 1166, 1167, 1587
AflIII	ACRYGT	4 cut(s)	423, 1105, 1771, 1893
AgeI	ACCGGT	2 cut(s)	710, 1340
AgsI	TTSAA	9 cut(s)	148, 436, 582, 722, 824, 1123, 1153, 1485, 1877
AjiI	CACGTC	1 cut(s)	1108
AjnI	CCWGG	4 cut(s)	127, 268, 1672, 1753
Alw21I	GWGCWC	1 cut(s)	288
Alw26I	GTCTC	4 cut(s)	342, 376, 461, 476
AlwI	GGATC	3 cut(s)	659, 1308, 1321
AlwNI	CAGNNNCTG	1 cut(s)	374
Ama87I	CYCGRG	1 cut(s)	656
AoxI	GGCC	5 cut(s)	84, 529, 795, 1410, 1615
ApeKI	GCWGC	9 cut(s)	55, 222, 725, 776, 803, 945, 1435, 1945, 2127
ApoI	RAATTY	4 cut(s)	431, 1536, 1749, 1954
ArsI	GACNNNNNNTTYG	2 cut(s)	354, 386
AseI	ATTAAT	1 cut(s)	1092
AsiGI	ACCGGT	2 cut(s)	710, 1340
Asp700I	GAANNNNTTC	1 cut(s)	1004
AspA2I	CCTAGG	2 cut(s)	940, 1994
AspLEI	GCGC	1 cut(s)	1356
AspS9I	GGNCC	5 cut(s)	257, 599, 652, 1591, 1670
AsuC2I	CCSGG	4 cut(s)	52, 60, 799, 1507
AsuHPI	GGTGA	4 cut(s)	104, 700, 1147, 1642
AvaI	CYCGRG	1 cut(s)	656
AvaII	GGWCC	5 cut(s)	257, 599, 652, 1591, 1670
AvrII	CCTAGG	2 cut(s)	940, 1994
AxyI	CCTNAGG	1 cut(s)	88
BamHI	GGATCC	1 cut(s)	1313
BanII	GRGCYC	2 cut(s)	8, 288
BbsI	GAAGAC	2 cut(s)	812, 2050
Bbv12I	GWGCWC	1 cut(s)	288
BbvI	GCAGC	9 cut(s)	42, 234, 712, 763, 815, 932, 1447, 1932, 2114
BccI	CCATC	4 cut(s)	815, 1109, 1262, 1279
BceAI	ACGGC	4 cut(s)	544, 1004, 1346, 1967
BciT130I	CCWGG	4 cut(s)	129, 270, 1674, 1755
BciVI	GTATCC	2 cut(s)	1250, 1743
BclI	TGATCA	2 cut(s)	1422, 1632
BcnI	CCSGG	4 cut(s)	52, 60, 799, 1507
BcoDI	GTCTC	4 cut(s)	342, 376, 461, 476
BfmI	CTRYAG	2 cut(s)	462, 1433
BfuAI	ACCTGC	2 cut(s)	443, 2122
BfuI	GTATCC	2 cut(s)	1250, 1743
BglII	AGATCT	1 cut(s)	1235
BisI	GCNGC	9 cut(s)	56, 223, 726, 777, 804, 946, 1436, 1946, 2128
BlnI	CCTAGG	2 cut(s)	940, 1994
BlsI	GCNGC	9 cut(s)	57, 224, 727, 778, 805, 947, 1437, 1947, 2129
BmcAI	AGTACT	1 cut(s)	332
Bme1390I	CCNGG	8 cut(s)	52, 60, 129, 270, 799, 1507, 1674, 1755
Bme18I	GGWCC	5 cut(s)	257, 599, 652, 1591, 1670
BmeT110I	CYCGRG	1 cut(s)	656
BmgBI	CACGTC	1 cut(s)	1108
BmgT120I	GGNCC	5 cut(s)	257, 599, 652, 1591, 1670
BmiI	GGNNCC	5 cut(s)	133, 654, 1315, 1579, 1592
BmrFI	CCNGG	8 cut(s)	52, 60, 129, 270, 799, 1507, 1674, 1755
BmsI	GCATC	2 cut(s)	1610, 2074
BpiI	GAAGAC	2 cut(s)	812, 2050
BpmI	CTGGAG	1 cut(s)	111
Bpu10I	CCTNAGC	1 cut(s)	1520
BpuEI	CTTGAG	2 cut(s)	230, 326
BpuMI	CCSGG	4 cut(s)	52, 60, 799, 1507
Bsa29I	ATCGAT	1 cut(s)	198
BsaAI	YACGTR	1 cut(s)	1772
BsaI	GGTCTC	1 cut(s)	461
BsaJI	CCNNGG	8 cut(s)	50, 268, 657, 798, 917, 940, 1605, 1994
BsaWI	WCCGGW	2 cut(s)	710, 1340
BsaXI	ACNNNNNCTCC	4 cut(s)	2040, 2064, 2070, 2094
Bsc4I	CCNNNNNNNGG	7 cut(s)	58, 128, 527, 1132, 1166, 1167, 1587
Bse118I	RCCGGY	2 cut(s)	710, 1340
Bse21I	CCTNAGG	1 cut(s)	88
Bse3DI	GCAATG	1 cut(s)	1296
BseBI	CCWGG	4 cut(s)	129, 270, 1674, 1755
BseCI	ATCGAT	1 cut(s)	198
BseDI	CCNNGG	8 cut(s)	50, 268, 657, 798, 917, 940, 1605, 1994
BseGI	GGATG	3 cut(s)	963, 1601, 2089
BseLI	CCNNNNNNNGG	7 cut(s)	58, 128, 527, 1132, 1166, 1167, 1587
BseMI	GCAATG	1 cut(s)	1296
BseMII	CTCAG	7 cut(s)	102, 254, 290, 497, 536, 1511, 1742
BseRI	GAGGAG	2 cut(s)	1017, 1955
BseXI	GCAGC	9 cut(s)	42, 234, 712, 763, 815, 932, 1447, 1932, 2114
BsgI	GTGCAG	1 cut(s)	387
Bsh1236I	CGCG	2 cut(s)	1214, 1354
BshFI	GGCC	5 cut(s)	86, 531, 797, 1412, 1617
BshTI	ACCGGT	2 cut(s)	710, 1340
BshVI	ATCGAT	1 cut(s)	198
BsiHKAI	GWGCWC	1 cut(s)	288
BsiHKCI	CYCGRG	1 cut(s)	656
BsiSI	CCGG	6 cut(s)	52, 59, 711, 798, 1341, 1506
BslFI	GGGAC	4 cut(s)	75, 90, 589, 849
BslI	CCNNNNNNNGG	7 cut(s)	58, 128, 527, 1132, 1166, 1167, 1587
BsmAI	GTCTC	4 cut(s)	342, 376, 461, 476
BsmBI	CGTCTC	1 cut(s)	342
BsmFI	GGGAC	4 cut(s)	75, 90, 589, 849
BsmI	GAATGC	2 cut(s)	16, 477
BsnI	GGCC	5 cut(s)	86, 531, 797, 1412, 1617
Bso31I	GGTCTC	1 cut(s)	461
BsoBI	CYCGRG	1 cut(s)	656
Bsp1286I	GDGCHC	2 cut(s)	8, 288
Bsp143I	GATC	7 cut(s)	199, 664, 1235, 1313, 1422, 1632, 1959
Bsp19I	CCATGG	1 cut(s)	1605
BspACI	CCGC	2 cut(s)	1214, 1628
BspANI	GGCC	5 cut(s)	86, 531, 797, 1412, 1617
BspCNI	CTCAG	7 cut(s)	101, 253, 291, 496, 535, 1512, 1741
BspDI	ATCGAT	1 cut(s)	198
BspFNI	CGCG	2 cut(s)	1214, 1354
BspHI	TCATGA	2 cut(s)	1299, 1761
BspLI	GGNNCC	5 cut(s)	133, 654, 1315, 1579, 1592
BspMAI	CTGCAG	1 cut(s)	1437
BspMI	ACCTGC	2 cut(s)	443, 2122
BspPI	GGATC	3 cut(s)	659, 1308, 1321
BspQI	GCTCTTC	1 cut(s)	13
BspTNI	GGTCTC	1 cut(s)	461
BsrDI	GCAATG	1 cut(s)	1296
BsrFI	RCCGGY	2 cut(s)	710, 1340
BssAI	RCCGGY	2 cut(s)	710, 1340
BssECI	CCNNGG	8 cut(s)	50, 268, 657, 798, 917, 940, 1605, 1994
BssMI	GATC	7 cut(s)	199, 664, 1235, 1313, 1422, 1632, 1959
BssT1I	CCWWGG	4 cut(s)	917, 940, 1605, 1994
Bst2UI	CCWGG	4 cut(s)	129, 270, 1674, 1755
Bst4CI	ACNGT	3 cut(s)	1149, 1420, 2068
Bst6I	CTCTTC	4 cut(s)	13, 18, 624, 743
BstAPI	GCANNNNNTGC	2 cut(s)	1478, 2084
BstBAI	YACGTR	1 cut(s)	1772
BstC8I	GCNNGC	3 cut(s)	1017, 1570, 2125
BstDEI	CTNAG	7 cut(s)	88, 240, 299, 483, 522, 1520, 1728
BstDSI	CCRYGG	1 cut(s)	1605
BstF5I	GGATG	3 cut(s)	963, 1601, 2089
BstFNI	CGCG	2 cut(s)	1214, 1354
BstHHI	GCGC	1 cut(s)	1356
BstKTI	GATC	7 cut(s)	202, 667, 1238, 1316, 1425, 1635, 1962
BstMAI	GTCTC	4 cut(s)	342, 376, 461, 476
BstMBI	GATC	7 cut(s)	199, 664, 1235, 1313, 1422, 1632, 1959
BstMWI	GCNNNNNNNGC	7 cut(s)	55, 170, 506, 803, 1205, 1478, 2084
BstNI	CCWGG	4 cut(s)	129, 270, 1674, 1755
BstNSI	RCATGY	2 cut(s)	427, 1897
BstSCI	CCNGG	8 cut(s)	50, 58, 127, 268, 797, 1505, 1672, 1753
BstSFI	CTRYAG	2 cut(s)	462, 1433
BstUI	CGCG	2 cut(s)	1214, 1354
BstV1I	GCAGC	9 cut(s)	42, 234, 712, 763, 815, 932, 1447, 1932, 2114
BstV2I	GAAGAC	2 cut(s)	812, 2050
BstX2I	RGATCY	2 cut(s)	1235, 1313
BstXI	CCANNNNNNTGG	2 cut(s)	1268, 2035
BstYI	RGATCY	2 cut(s)	1235, 1313
Bsu15I	ATCGAT	1 cut(s)	198
Bsu36I	CCTNAGG	1 cut(s)	88
BsuI	GTATCC	2 cut(s)	1250, 1743
BsuRI	GGCC	5 cut(s)	86, 531, 797, 1412, 1617
BsuTUI	ATCGAT	1 cut(s)	198
BtgI	CCRYGG	1 cut(s)	1605
BtrI	CACGTC	1 cut(s)	1108
BtsCI	GGATG	3 cut(s)	963, 1601, 2089
BtsI	GCAGTG	5 cut(s)	159, 693, 1430, 1722, 2085
BtsIMutI	CAGTG	8 cut(s)	159, 248, 693, 1378, 1430, 1722, 2085, 2094
BveI	ACCTGC	2 cut(s)	443, 2122
Cac8I	GCNNGC	3 cut(s)	1017, 1570, 2125
CaiI	CAGNNNCTG	1 cut(s)	374
CciI	TCATGA	2 cut(s)	1299, 1761
CfoI	GCGC	1 cut(s)	1356
Cfr10I	RCCGGY	2 cut(s)	710, 1340
Cfr13I	GGNCC	5 cut(s)	257, 599, 652, 1591, 1670
ClaI	ATCGAT	1 cut(s)	198
Csp6I	GTAC	5 cut(s)	331, 526, 548, 689, 1320
CspAI	ACCGGT	2 cut(s)	710, 1340
CspCI	CAANNNNNGTGG	2 cut(s)	899, 934
CviQI	GTAC	5 cut(s)	331, 526, 548, 689, 1320
DdeI	CTNAG	7 cut(s)	88, 240, 299, 483, 522, 1520, 1728
DpnI	GATC	7 cut(s)	201, 666, 1237, 1315, 1424, 1634, 1961
DpnII	GATC	7 cut(s)	199, 664, 1235, 1313, 1422, 1632, 1959
EaeI	YGGCCR	1 cut(s)	795
Eam1104I	CTCTTC	4 cut(s)	13, 18, 624, 743
EarI	CTCTTC	4 cut(s)	13, 18, 624, 743
Ecl136II	GAGCTC	1 cut(s)	286
Eco130I	CCWWGG	4 cut(s)	917, 940, 1605, 1994
Eco147I	AGGCCT	1 cut(s)	86
Eco24I	GRGCYC	2 cut(s)	8, 288
Eco31I	GGTCTC	1 cut(s)	461
Eco47I	GGWCC	5 cut(s)	257, 599, 652, 1591, 1670
Eco53kI	GAGCTC	1 cut(s)	286
Eco57I	CTGAAG	3 cut(s)	42, 724, 1354
Eco81I	CCTNAGG	1 cut(s)	88
Eco88I	CYCGRG	1 cut(s)	656
EcoICRI	GAGCTC	1 cut(s)	286
EcoO109I	RGGNCCY	2 cut(s)	652, 1591
EcoRII	CCWGG	4 cut(s)	127, 268, 1672, 1753
EcoT14I	CCWWGG	4 cut(s)	917, 940, 1605, 1994
EcoT38I	GRGCYC	2 cut(s)	8, 288
ErhI	CCWWGG	4 cut(s)	917, 940, 1605, 1994
Esp3I	CGTCTC	1 cut(s)	342
FalI	AAGNNNNNCTT	6 cut(s)	102, 134, 1544, 1576, 1769, 1801
FaqI	GGGAC	4 cut(s)	75, 90, 589, 849
FbaI	TGATCA	2 cut(s)	1422, 1632
FblI	GTMKAC	1 cut(s)	1530
Fnu4HI	GCNGC	9 cut(s)	56, 223, 726, 777, 804, 946, 1436, 1946, 2128
FokI	GGATG	3 cut(s)	950, 1588, 2096
FriOI	GRGCYC	2 cut(s)	8, 288
Fsp4HI	GCNGC	9 cut(s)	56, 223, 726, 777, 804, 946, 1436, 1946, 2128
GlaI	GCGC	1 cut(s)	1355
GluI	GCNGC	9 cut(s)	56, 223, 726, 777, 804, 946, 1436, 1946, 2128
GsuI	CTGGAG	1 cut(s)	111
HaeIII	GGCC	5 cut(s)	86, 531, 797, 1412, 1617
HapII	CCGG	6 cut(s)	52, 59, 711, 798, 1341, 1506
HhaI	GCGC	1 cut(s)	1356
Hin6I	GCGC	1 cut(s)	1354
HinP1I	GCGC	1 cut(s)	1354
HincII	GTYRAC	2 cut(s)	1366, 1443
HindII	GTYRAC	2 cut(s)	1366, 1443
HindIII	AAGCTT	1 cut(s)	512
HpaI	GTTAAC	1 cut(s)	1366
HpaII	CCGG	6 cut(s)	52, 59, 711, 798, 1341, 1506
HphI	GGTGA	4 cut(s)	104, 700, 1147, 1642
Hpy166II	GTNNAC	4 cut(s)	78, 1366, 1443, 1531
Hpy8I	GTNNAC	4 cut(s)	78, 1366, 1443, 1531
Hpy99I	CGWCG	1 cut(s)	2072
HpyAV	CCTTC	7 cut(s)	128, 739, 1537, 1591, 1628, 1961, 2046
HpyCH4III	ACNGT	3 cut(s)	1149, 1420, 2068
HpyCH4IV	ACGT	5 cut(s)	701, 1107, 1305, 1401, 1771
HpyF10VI	GCNNNNNNNGC	7 cut(s)	55, 170, 506, 803, 1205, 1478, 2084
HpyF3I	CTNAG	7 cut(s)	88, 240, 299, 483, 522, 1520, 1728
HpySE526I	ACGT	5 cut(s)	701, 1107, 1305, 1401, 1771
HspAI	GCGC	1 cut(s)	1354
Ksp22I	TGATCA	2 cut(s)	1422, 1632
KspAI	GTTAAC	1 cut(s)	1366
Kzo9I	GATC	7 cut(s)	199, 664, 1235, 1313, 1422, 1632, 1959
LguI	GCTCTTC	1 cut(s)	13
LmnI	GCTCC	4 cut(s)	130, 1030, 1577, 1942
Lsp1109I	GCAGC	9 cut(s)	42, 234, 712, 763, 815, 932, 1447, 1932, 2114
LweI	GCATC	2 cut(s)	1610, 2074
MaeII	ACGT	5 cut(s)	701, 1107, 1305, 1401, 1771
MaeIII	GTNAC	4 cut(s)	645, 1143, 1271, 2062
MalI	GATC	7 cut(s)	201, 666, 1237, 1315, 1424, 1634, 1961
MboI	GATC	7 cut(s)	199, 664, 1235, 1313, 1422, 1632, 1959
MfeI	CAATTG	3 cut(s)	594, 1221, 1665
MflI	RGATCY	2 cut(s)	1235, 1313
MhlI	GDGCHC	2 cut(s)	8, 288
MlyI	GAGTC	5 cut(s)	232, 287, 729, 931, 1045
MmeI	TCCRAC	5 cut(s)	884, 1210, 1536, 2050, 2052
MroXI	GAANNNNTTC	1 cut(s)	1004
MseI	TTAA	7 cut(s)	446, 1092, 1101, 1365, 1515, 1746, 1908
MslI	CAYNNNNRTG	3 cut(s)	955, 968, 1266
MspA1I	CMGCKG	1 cut(s)	1438
MspI	CCGG	6 cut(s)	52, 59, 711, 798, 1341, 1506
MspR9I	CCNGG	8 cut(s)	52, 60, 129, 270, 799, 1507, 1674, 1755
MunI	CAATTG	3 cut(s)	594, 1221, 1665
Mva1269I	GAATGC	2 cut(s)	16, 477
MvaI	CCWGG	4 cut(s)	129, 270, 1674, 1755
MvnI	CGCG	2 cut(s)	1214, 1354
MwoI	GCNNNNNNNGC	7 cut(s)	55, 170, 506, 803, 1205, 1478, 2084
NciI	CCSGG	4 cut(s)	52, 60, 799, 1507
NcoI	CCATGG	1 cut(s)	1605
NdeII	GATC	7 cut(s)	199, 664, 1235, 1313, 1422, 1632, 1959
NlaIV	GGNNCC	5 cut(s)	133, 654, 1315, 1579, 1592
NmuCI	GTSAC	1 cut(s)	2062
NspI	RCATGY	2 cut(s)	427, 1897
PagI	TCATGA	2 cut(s)	1299, 1761
PaqCI	CACCTGC	1 cut(s)	2122
PceI	AGGCCT	1 cut(s)	86
PciI	ACATGT	2 cut(s)	423, 1893
PciSI	GCTCTTC	1 cut(s)	13
PcsI	WCGNNNNNNNCGW	1 cut(s)	789
PctI	GAATGC	2 cut(s)	16, 477
PdmI	GAANNNNTTC	1 cut(s)	1004
PfeI	GAWTC	9 cut(s)	346, 397, 637, 846, 1138, 1296, 1758, 2008, 2091
PfoI	TCCNGGA	1 cut(s)	1753
PinAI	ACCGGT	2 cut(s)	710, 1340
PkrI	GCNGC	9 cut(s)	57, 224, 727, 778, 805, 947, 1437, 1947, 2129
PleI	GAGTC	5 cut(s)	232, 287, 729, 930, 1045
PpsI	GAGTC	5 cut(s)	232, 287, 729, 930, 1045
Ppu21I	YACGTR	1 cut(s)	1772
PpuMI	RGGWCCY	2 cut(s)	652, 1591
PscI	ACATGT	2 cut(s)	423, 1893
PshBI	ATTAAT	1 cut(s)	1092
Psp124BI	GAGCTC	1 cut(s)	288
Psp1406I	AACGTT	1 cut(s)	701
Psp5II	RGGWCCY	2 cut(s)	652, 1591
Psp6I	CCWGG	4 cut(s)	127, 268, 1672, 1753
PspGI	CCWGG	4 cut(s)	127, 268, 1672, 1753
PspN4I	GGNNCC	5 cut(s)	133, 654, 1315, 1579, 1592
PspPI	GGNCC	5 cut(s)	257, 599, 652, 1591, 1670
PspPPI	RGGWCCY	2 cut(s)	652, 1591
PstI	CTGCAG	1 cut(s)	1437
PstNI	CAGNNNCTG	1 cut(s)	374
PsuI	RGATCY	2 cut(s)	1235, 1313
PvuII	CAGCTG	1 cut(s)	1438
RsaI	GTAC	5 cut(s)	332, 527, 549, 690, 1321
RsaNI	GTAC	5 cut(s)	331, 526, 548, 689, 1320
RseI	CAYNNNNRTG	3 cut(s)	955, 968, 1266
SacI	GAGCTC	1 cut(s)	288
SapI	GCTCTTC	1 cut(s)	13
SaqAI	TTAA	7 cut(s)	446, 1092, 1101, 1365, 1515, 1746, 1908
SatI	GCNGC	9 cut(s)	56, 223, 726, 777, 804, 946, 1436, 1946, 2128
Sau3AI	GATC	7 cut(s)	199, 664, 1235, 1313, 1422, 1632, 1959
Sau96I	GGNCC	5 cut(s)	257, 599, 652, 1591, 1670
ScaI	AGTACT	1 cut(s)	332
SchI	GAGTC	5 cut(s)	232, 287, 729, 931, 1045
ScrFI	CCNGG	8 cut(s)	52, 60, 129, 270, 799, 1507, 1674, 1755
SduI	GDGCHC	2 cut(s)	8, 288
SfaNI	GCATC	2 cut(s)	1610, 2074
SfcI	CTRYAG	2 cut(s)	462, 1433
SinI	GGWCC	5 cut(s)	257, 599, 652, 1591, 1670
SmiMI	CAYNNNNRTG	3 cut(s)	955, 968, 1266
SmlI	CTYRAG	2 cut(s)	209, 341
SmoI	CTYRAG	2 cut(s)	209, 341
SseBI	AGGCCT	1 cut(s)	86
SsiI	CCGC	2 cut(s)	1214, 1628
SspI	AATATT	1 cut(s)	1096
SstI	GAGCTC	1 cut(s)	288
StuI	AGGCCT	1 cut(s)	86
StyD4I	CCNGG	8 cut(s)	50, 58, 127, 268, 797, 1505, 1672, 1753
StyI	CCWWGG	4 cut(s)	917, 940, 1605, 1994
TaaI	ACNGT	3 cut(s)	1149, 1420, 2068
TaiI	ACGT	5 cut(s)	704, 1110, 1308, 1404, 1774
TaqI	TCGA	2 cut(s)	198, 1049
TaqII	GACCGA	1 cut(s)	806
TatI	WGTACW	2 cut(s)	330, 1319
TfiI	GAWTC	9 cut(s)	346, 397, 637, 846, 1138, 1296, 1758, 2008, 2091
Tru1I	TTAA	7 cut(s)	446, 1092, 1101, 1365, 1515, 1746, 1908
Tru9I	TTAA	7 cut(s)	446, 1092, 1101, 1365, 1515, 1746, 1908
TscAI	CASTG	8 cut(s)	166, 248, 700, 1378, 1437, 1729, 2085, 2101
TseFI	GTSAC	1 cut(s)	2062
TseI	GCWGC	9 cut(s)	55, 222, 725, 776, 803, 945, 1435, 1945, 2127
Tsp45I	GTSAC	1 cut(s)	2062
TspDTI	ATGAA	5 cut(s)	952, 1265, 1288, 1316, 1335
TspRI	CASTG	8 cut(s)	166, 248, 700, 1378, 1437, 1729, 2085, 2101
VpaK11BI	GGWCC	5 cut(s)	257, 599, 652, 1591, 1670
VspI	ATTAAT	1 cut(s)	1092
XapI	RAATTY	4 cut(s)	431, 1536, 1749, 1954
XbaI	TCTAGA	1 cut(s)	1936
XceI	RCATGY	2 cut(s)	427, 1897
XmaJI	CCTAGG	2 cut(s)	940, 1994
XmiI	GTMKAC	1 cut(s)	1530
XmnI	GAANNNNTTC	1 cut(s)	1004
ZrmI	AGTACT	1 cut(s)	332

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.