MD01G1054300.v1.1

Acetyl-coenzyme A carboxylase carboxyl transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012647
Physical Location & Seq
Reverse (-)
15876653 .. 15880049
3397 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1054300.v1.1.491

Sequence Viewer

Length: 1368 bp
ATGACTACCCTATCACTAATTGCTGGAAATTGTGGAAGAAGAAGCAGAGGAGAAGATCGTGGATTAGAATTGTTCCATCCGTTTTCAAGAAAGGATCTTTTTGCTAGTGAATTGCTCAGAAGTTCTTTTACAAGGGCAAATGGAATCAGGTCCAAGGATTTGGAAGGTCATAGGATTAGAAACCAGAGGAAGTTTCGTGTTAGCGCAAAAATTAAAAAGGGGAAGAACTGTGATTATCTATGGCCTCATAATATTGATCCAAACATCAGCAGCGGCCACTTGAGTTACCTGTTTCATTTCAAGCCTCTGACTGAGAAGCCAAAGCAAGTTACCCTACCATTCGAGAAGCCACTTGTTGATCTCAATAAAAAGTTTACTGAGATACGTAGAATGGCTGATGAAACTAGTCGGGACTTCAGCGATCAAATTGCTACACTGGAGAATAAGTATCAACAGTGGGTGGAACTACATGGATATCGTGCTTGCTATGATGATCCGGCTATTGTGACAGGTCTTGGGAGCATGGATGGCCAATCCATGTTTATAGGTCATCAGAAAGGTAGAAACACCAAGGAGAACATTGCTTGCAACTTTGCAATGCCAACTCCTCATGACTATCGGAAGGTTCTCAGAATGATGAAATATGCCGATCATCACAGATTTCCCATTGTTACGTTTGTTGACACTCCTGGGGCCTTTGCTGATCTCAAATCTGAGGAACTTGGTCAAAATTTGAGGACTATGTTTGGCCTGAAAGTGCCCATTGTAATGGTTGTAACTGGAGAAGGTGGTTTAGGTGGCGTTCTAGCCATTGCCTGTTCCAATAAACTGTTTATGCTGGAGAACTCTGCTTTCTATATTGCAAGCCCTGAAGCATGCGCTGCAATATTGTGGAAATCTTCCCAAATAGCTCCTAAGGTAGCAGAAAAACTAAGGATAATGACTCAGAAGCATTATAGACTGAAAATCGCCGATGGGATCATTCCCCTGTTACAGGAGCCTCTTGGTGGCGCGCATGCTGATCCTGCATGGACTTCCCAACAAATTAAGACAACAATCACACAAGCAATAAAGGAGCTGGAAAAAATAAATACAGAAGAGTTGCTTCAGCATCGGAGGCTTAAATTCCGATTAATAAGAGGTTATCAAGAAGGCATTCCAATGGAACTGAAGAGGAAACACAACATTAAGCCCTCCGAAGTTAACATGCCAAAGGCTGCTGACTTAGAGGCAGAAATTGAGAATTTAAAAAAGAAGATCCTGGAAGCAAAGGGACCATCCGATCCAGTTACAATCCAAACAATTGAAAAACTGAAGCAAGACGTAGACAAGGAGATGGCAAATGCATTTATTTCGATGGGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
ACCA

Protein Analysis

456

Amino Acids

51.62

Weight (kDa)

9.42

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACCA PF03255 151 - 378 7.4e-81 Acetyl co-enzyme A carboxylase carboxyltransferase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1326
AccII CGCG 1 cut(s) 1013
AciI CCGC 1 cut(s) 273
AclWI GGATC 7 cut(s) 102, 251, 488, 986, 1016, 1252, 1277
AcoI YGGCCR 2 cut(s) 274, 529
AcsI RAATTY 3 cut(s) 730, 1124, 1243
AcuI CTGAAG 5 cut(s) 400, 891, 1091, 1190, 1334
AfiI CCNNNNNNNGG 2 cut(s) 994, 1007
AgsI TTSAA 3 cut(s) 87, 301, 1307
AhlI ACTAGT 1 cut(s) 404
AjnI CCWGG 2 cut(s) 688, 1260
AluBI AGCT 2 cut(s) 911, 1078
AluI AGCT 2 cut(s) 911, 1078
AlwI GGATC 7 cut(s) 102, 251, 488, 986, 1016, 1252, 1277
AoxI GGCC 5 cut(s) 242, 274, 529, 693, 748
ApeKI GCWGC 3 cut(s) 270, 881, 1217
ApoI RAATTY 3 cut(s) 730, 1124, 1243
AseI ATTAAT 1 cut(s) 1133
Asp700I GAANNNNTTC 1 cut(s) 1155
AspLEI GCGC 4 cut(s) 206, 881, 1013, 1015
AspS9I GGNCC 3 cut(s) 150, 693, 1274
AvaII GGWCC 2 cut(s) 150, 1274
AxyI CCTNAGG 1 cut(s) 915
BaeGI GKGCMC 1 cut(s) 762
BalI TGGCCA 1 cut(s) 531
BarI GAAGNNNNNNTAC 2 cut(s) 112, 144
BbvI GCAGC 3 cut(s) 282, 868, 1204
BccI CCATC 6 cut(s) 84, 521, 968, 1285, 1330, 1351
BcgI CGANNNNNNTGC 3 cut(s) 410, 444, 1335
BciT130I CCWGG 2 cut(s) 690, 1262
BcuI ACTAGT 1 cut(s) 404
BfaI CTAG 3 cut(s) 105, 405, 806
BisI GCNGC 4 cut(s) 271, 274, 882, 1218
BlsI GCNGC 4 cut(s) 272, 275, 883, 1219
Bme1390I CCNGG 2 cut(s) 690, 1262
Bme18I GGWCC 2 cut(s) 150, 1274
BmgT120I GGNCC 3 cut(s) 150, 693, 1274
BmiI GGNNCC 3 cut(s) 694, 999, 1275
BmrFI CCNGG 2 cut(s) 690, 1262
BmsI GCATC 1 cut(s) 1120
BpmI CTGGAG 3 cut(s) 458, 801, 860
BpuEI CTTGAG 1 cut(s) 301
BsaAI YACGTR 1 cut(s) 386
BsaJI CCNNGG 3 cut(s) 153, 570, 689
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 2 cut(s) 994, 1007
Bse1I ACTGG 3 cut(s) 441, 784, 1286
Bse21I CCTNAGG 1 cut(s) 915
Bse3DI GCAATG 3 cut(s) 579, 603, 810
BseBI CCWGG 2 cut(s) 690, 1262
BseDI CCNNGG 3 cut(s) 153, 570, 689
BseGI GGATG 3 cut(s) 76, 532, 1277
BseLI CCNNNNNNNGG 2 cut(s) 994, 1007
BseMI GCAATG 3 cut(s) 579, 603, 810
BseMII CTCAG 6 cut(s) 130, 303, 369, 643, 705, 959
BseNI ACTGG 3 cut(s) 441, 784, 1286
BsePI GCGCGC 1 cut(s) 1011
BseRI GAGGAG 2 cut(s) 63, 597
BseSI GKGCMC 1 cut(s) 762
BseXI GCAGC 3 cut(s) 282, 868, 1204
Bsh1236I CGCG 1 cut(s) 1013
BshFI GGCC 5 cut(s) 244, 276, 531, 695, 750
BsiSI CCGG 1 cut(s) 497
BslFI GGGAC 2 cut(s) 425, 1287
BslI CCNNNNNNNGG 2 cut(s) 994, 1007
BsmFI GGGAC 2 cut(s) 425, 1287
BsmI GAATGC 1 cut(s) 1155
BsnI GGCC 5 cut(s) 244, 276, 531, 695, 750
Bsp1286I GDGCHC 1 cut(s) 762
BspACI CCGC 1 cut(s) 273
BspANI GGCC 5 cut(s) 244, 276, 531, 695, 750
BspCNI CTCAG 6 cut(s) 129, 304, 370, 642, 706, 958
BspFNI CGCG 1 cut(s) 1013
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 3 cut(s) 694, 999, 1275
BspPI GGATC 7 cut(s) 102, 251, 488, 986, 1016, 1252, 1277
BsrDI GCAATG 3 cut(s) 579, 603, 810
BsrI ACTGG 3 cut(s) 441, 784, 1286
BssECI CCNNGG 3 cut(s) 153, 570, 689
BssHII GCGCGC 1 cut(s) 1011
BssT1I CCWWGG 2 cut(s) 153, 570
Bst2UI CCWGG 2 cut(s) 690, 1262
Bst4CI ACNGT 3 cut(s) 230, 456, 831
Bst6I CTCTTC 2 cut(s) 1092, 1166
BstAPI GCANNNNNTGC 1 cut(s) 881
BstBAI YACGTR 1 cut(s) 386
BstC8I GCNNGC 6 cut(s) 484, 586, 865, 877, 1013, 1017
BstDEI CTNAG 9 cut(s) 116, 312, 378, 629, 714, 915, 932, 945, 1225
BstENI CCTNNNNNAGG 1 cut(s) 992
BstF5I GGATG 3 cut(s) 76, 532, 1277
BstFNI CGCG 1 cut(s) 1013
BstHHI GCGC 4 cut(s) 206, 881, 1013, 1015
BstMWI GCNNNNNNNGC 4 cut(s) 528, 881, 1025, 1117
BstNI CCWGG 2 cut(s) 690, 1262
BstNSI RCATGY 3 cut(s) 879, 1019, 1210
BstSCI CCNGG 2 cut(s) 688, 1260
BstSLI GKGCMC 1 cut(s) 762
BstSNI TACGTA 1 cut(s) 386
BstUI CGCG 1 cut(s) 1013
BstV1I GCAGC 3 cut(s) 282, 868, 1204
BstX2I RGATCY 2 cut(s) 94, 1257
BstXI CCANNNNNNTGG 2 cut(s) 160, 769
BstYI RGATCY 2 cut(s) 94, 1257
Bsu36I CCTNAGG 1 cut(s) 915
BsuRI GGCC 5 cut(s) 244, 276, 531, 695, 750
BtsCI GGATG 3 cut(s) 76, 532, 1277
BtsIMutI CAGTG 2 cut(s) 434, 461
Cac8I GCNNGC 6 cut(s) 484, 586, 865, 877, 1013, 1017
CciI TCATGA 1 cut(s) 610
CfoI GCGC 4 cut(s) 206, 881, 1013, 1015
Cfr13I GGNCC 3 cut(s) 150, 693, 1274
CviAII CATG 8 cut(s) 470, 523, 538, 611, 876, 1016, 1029, 1207
DdeI CTNAG 9 cut(s) 116, 312, 378, 629, 714, 915, 932, 945, 1225
DraI TTTAAA 1 cut(s) 1248
EaeI YGGCCR 2 cut(s) 274, 529
Eam1104I CTCTTC 2 cut(s) 1092, 1166
EarI CTCTTC 2 cut(s) 1092, 1166
Eco105I TACGTA 1 cut(s) 386
Eco130I CCWWGG 2 cut(s) 153, 570
Eco32I GATATC 1 cut(s) 476
Eco47I GGWCC 2 cut(s) 150, 1274
Eco57I CTGAAG 5 cut(s) 400, 891, 1091, 1190, 1334
Eco81I CCTNAGG 1 cut(s) 915
EcoNI CCTNNNNNAGG 1 cut(s) 992
EcoO109I RGGNCCY 1 cut(s) 693
EcoRII CCWGG 2 cut(s) 688, 1260
EcoRV GATATC 1 cut(s) 476
EcoT14I CCWWGG 2 cut(s) 153, 570
EcoT22I ATGCAT 1 cut(s) 1348
ErhI CCWWGG 2 cut(s) 153, 570
FaeI CATG 8 cut(s) 473, 526, 541, 614, 879, 1019, 1032, 1210
FalI AAGNNNNNCTT 2 cut(s) 1089, 1121
FaqI GGGAC 2 cut(s) 425, 1287
FatI CATG 8 cut(s) 469, 522, 537, 610, 875, 1015, 1028, 1206
FblI GTMKAC 1 cut(s) 1326
Fnu4HI GCNGC 4 cut(s) 271, 274, 882, 1218
FokI GGATG 3 cut(s) 63, 539, 1264
Fsp4HI GCNGC 4 cut(s) 271, 274, 882, 1218
FspBI CTAG 3 cut(s) 105, 405, 806
GlaI GCGC 4 cut(s) 205, 880, 1012, 1014
GluI GCNGC 4 cut(s) 271, 274, 882, 1218
GsuI CTGGAG 3 cut(s) 458, 801, 860
HaeIII GGCC 5 cut(s) 244, 276, 531, 695, 750
HapII CCGG 1 cut(s) 497
HhaI GCGC 4 cut(s) 206, 881, 1013, 1015
Hin1II CATG 8 cut(s) 473, 526, 541, 614, 879, 1019, 1032, 1210
Hin6I GCGC 4 cut(s) 204, 879, 1011, 1013
HinP1I GCGC 4 cut(s) 204, 879, 1011, 1013
HincII GTYRAC 2 cut(s) 682, 1204
HindII GTYRAC 2 cut(s) 682, 1204
HinfI GANTC 2 cut(s) 144, 943
HpaI GTTAAC 1 cut(s) 1204
HpaII CCGG 1 cut(s) 497
Hpy166II GTNNAC 4 cut(s) 375, 682, 1204, 1327
Hpy188III TCNNGA 5 cut(s) 87, 343, 410, 611, 1148
Hpy8I GTNNAC 4 cut(s) 375, 682, 1204, 1327
HpyAV CCTTC 4 cut(s) 158, 616, 779, 1145
HpyCH4III ACNGT 3 cut(s) 230, 456, 831
HpyCH4IV ACGT 3 cut(s) 385, 674, 1323
HpyCH4V TGCA 6 cut(s) 588, 596, 863, 884, 1028, 1346
HpyF10VI GCNNNNNNNGC 4 cut(s) 528, 881, 1025, 1117
HpyF3I CTNAG 9 cut(s) 116, 312, 378, 629, 714, 915, 932, 945, 1225
HpySE526I ACGT 3 cut(s) 385, 674, 1323
Hsp92II CATG 8 cut(s) 473, 526, 541, 614, 879, 1019, 1032, 1210
HspAI GCGC 4 cut(s) 204, 879, 1011, 1013
KspAI GTTAAC 1 cut(s) 1204
LmnI GCTCC 4 cut(s) 519, 916, 997, 1075
Lsp1109I GCAGC 3 cut(s) 282, 868, 1204
LweI GCATC 1 cut(s) 1120
MaeI CTAG 3 cut(s) 105, 405, 806
MaeII ACGT 3 cut(s) 385, 674, 1323
MaeIII GTNAC 7 cut(s) 284, 328, 505, 670, 775, 990, 1288
MboII GAAGA 8 cut(s) 48, 51, 65, 235, 891, 1109, 1183, 1267
MfeI CAATTG 1 cut(s) 1302
MflI RGATCY 2 cut(s) 94, 1257
MhlI GDGCHC 1 cut(s) 762
MlsI TGGCCA 1 cut(s) 531
MluNI TGGCCA 1 cut(s) 531
MlyI GAGTC 1 cut(s) 937
Mox20I TGGCCA 1 cut(s) 531
Mph1103I ATGCAT 1 cut(s) 1348
MroXI GAANNNNTTC 1 cut(s) 1155
MscI TGGCCA 1 cut(s) 531
MseI TTAA 7 cut(s) 213, 1047, 1122, 1133, 1188, 1203, 1247
MslI CAYNNNNRTG 2 cut(s) 767, 1160
Msp20I TGGCCA 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 273
MspI CCGG 1 cut(s) 497
MspR9I CCNGG 2 cut(s) 690, 1262
MunI CAATTG 1 cut(s) 1302
Mva1269I GAATGC 1 cut(s) 1155
MvaI CCWGG 2 cut(s) 690, 1262
MvnI CGCG 1 cut(s) 1013
MwoI GCNNNNNNNGC 4 cut(s) 528, 881, 1025, 1117
NlaIII CATG 8 cut(s) 473, 526, 541, 614, 879, 1019, 1032, 1210
NlaIV GGNNCC 3 cut(s) 694, 999, 1275
NmuCI GTSAC 1 cut(s) 505
NsiI ATGCAT 1 cut(s) 1348
NspI RCATGY 3 cut(s) 879, 1019, 1210
PaeI GCATGC 2 cut(s) 879, 1019
PagI TCATGA 1 cut(s) 610
PauI GCGCGC 1 cut(s) 1011
PctI GAATGC 1 cut(s) 1155
PdmI GAANNNNTTC 1 cut(s) 1155
PfeI GAWTC 1 cut(s) 144
PfoI TCCNGGA 1 cut(s) 1260
PkrI GCNGC 4 cut(s) 272, 275, 883, 1219
PleI GAGTC 1 cut(s) 937
PpsI GAGTC 1 cut(s) 937
Ppu21I YACGTR 1 cut(s) 386
PshBI ATTAAT 1 cut(s) 1133
Psp6I CCWGG 2 cut(s) 688, 1260
PspGI CCWGG 2 cut(s) 688, 1260
PspN4I GGNNCC 3 cut(s) 694, 999, 1275
PspPI GGNCC 3 cut(s) 150, 693, 1274
PsuI RGATCY 2 cut(s) 94, 1257
PteI GCGCGC 1 cut(s) 1011
RseI CAYNNNNRTG 2 cut(s) 767, 1160
SaqAI TTAA 7 cut(s) 213, 1047, 1122, 1133, 1188, 1203, 1247
SatI GCNGC 4 cut(s) 271, 274, 882, 1218
Sau96I GGNCC 3 cut(s) 150, 693, 1274
SchI GAGTC 1 cut(s) 937
ScrFI CCNGG 2 cut(s) 690, 1262
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 1 cut(s) 1120
SinI GGWCC 2 cut(s) 150, 1274
SmiMI CAYNNNNRTG 2 cut(s) 767, 1160
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
SnaBI TACGTA 1 cut(s) 386
SpeI ACTAGT 1 cut(s) 404
SphI GCATGC 2 cut(s) 879, 1019
SsiI CCGC 1 cut(s) 273
SspI AATATT 2 cut(s) 253, 888
SspMI CTAG 3 cut(s) 105, 405, 806
StyD4I CCNGG 2 cut(s) 688, 1260
StyI CCWWGG 2 cut(s) 153, 570
TaaI ACNGT 3 cut(s) 230, 456, 831
TaiI ACGT 3 cut(s) 388, 677, 1326
TaqI TCGA 2 cut(s) 342, 1355
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 7 cut(s) 213, 1047, 1122, 1133, 1188, 1203, 1247
Tru9I TTAA 7 cut(s) 213, 1047, 1122, 1133, 1188, 1203, 1247
TscAI CASTG 2 cut(s) 441, 461
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 3 cut(s) 270, 881, 1217
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 3 cut(s) 284, 414, 653
TspGWI ACGGA 1 cut(s) 69
TspRI CASTG 2 cut(s) 441, 461
VpaK11BI GGWCC 2 cut(s) 150, 1274
VspI ATTAAT 1 cut(s) 1133
XagI CCTNNNNNAGG 1 cut(s) 992
XapI RAATTY 3 cut(s) 730, 1124, 1243
XceI RCATGY 3 cut(s) 879, 1019, 1210
XmiI GTMKAC 1 cut(s) 1326
XmnI GAANNNNTTC 1 cut(s) 1155
XspI CTAG 3 cut(s) 105, 405, 806
Zsp2I ATGCAT 1 cut(s) 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.