MD01G1097100.v1.1

Belongs to the isocitrate and isopropylmalate dehydrogenases family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0020386
Physical Location & Seq
Reverse (-)
21156058 .. 21159438
3381 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1097100.v1.1.491

Sequence Viewer

Length: 756 bp
ATGGAAAGGTGGTTAATGAAATTGTATTGTTTGTATTTTATAATTTCGGTTGCAAAATCTGGTGAAAATGATGCCCACCATGTGTTTACTGGACTGCAGCAAACGATCGACATTGCTTCTTTGAAGCTTGGCCTCCATAAATTCATTTGTGACAAAGGTGGAGGTTTACCAAAAGACAAGATTCGGAAGGACCACTCAAGAATATCCAAGAATTTTTTGGAAGATATTGAACTCTTAATTGTACCTGCTGACCAAATTCGTATCACTCTCCAAGTGTTTGATGAAAGGTCACACTCAGCAGATTGTGATTTTCAGTTAGGCTATCACATGCCGATTTCTGACACCGGAGGTACACTTCTTAGGTTCTTTACTTCAAATGTTCAAATCAAAGATGCTCATAGTTCTAAATTTTTTGTTTGTAATGGTGAAGTCATGAAAAATGGTACTATTGTGCATTCGAAGCTTGGTTTCCATGAGTTAATTCGTGACAAGGGTGGAACAATTAGTCAGAGGTTCTTCAATTCGAAGTCCAAGAATAAAGATTGTGTTTCTGATTGTTCCAAGGTGGTCAAATGTGAGGAGCCGGGATGGTCCTGGATAAAGGTCAAGGATCGACTTTCTGCAATTGTGGGTGGAGATCGGTCTCATCCACAAGTTGATGATAGATACAATGTGTTGGAATTACTAGCTTCAAAGGCAGAAGATATAATTCAGAAAATTGGTTCTTCACTAATTGACGTTGAAGATTGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146
Pfam Domains
Protein Families
Iso_dh

Protein Analysis

252

Amino Acids

28.44

Weight (kDa)

7.13

Isoelectric Point (pI)

26.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020386)

Species Orthologous Gene IDs
malus_domestica MD01G1097100.v1.1
pyrus_communis pycom09g14330 pycom12g02830 pycom13g19400 pycom13g28690 pycom15g18780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 41
Acc36I ACCTGC 1 cut(s) 253
AclWI GGATC 1 cut(s) 618
AcsI RAATTY 4 cut(s) 140, 211, 255, 407
AdeI CACNNNGTG 1 cut(s) 82
AfaI GTAC 3 cut(s) 243, 352, 445
AgsI TTSAA 7 cut(s) 124, 230, 375, 383, 520, 693, 743
AjnI CCWGG 1 cut(s) 593
AluBI AGCT 3 cut(s) 127, 463, 689
AluI AGCT 3 cut(s) 127, 463, 689
Alw26I GTCTC 1 cut(s) 648
AlwI GGATC 1 cut(s) 618
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 1 cut(s) 97
ApoI RAATTY 4 cut(s) 140, 211, 255, 407
AspS9I GGNCC 2 cut(s) 190, 591
AsuC2I CCSGG 1 cut(s) 585
AsuHPI GGTGA 2 cut(s) 74, 437
AsuII TTCGAA 2 cut(s) 458, 524
AvaII GGWCC 2 cut(s) 190, 591
BaeI ACNNNNGTAYC 2 cut(s) 435, 468
BbvI GCAGC 1 cut(s) 109
BccI CCATC 1 cut(s) 582
BciT130I CCWGG 1 cut(s) 595
BcnI CCSGG 1 cut(s) 585
BcoDI GTCTC 1 cut(s) 648
BfaI CTAG 1 cut(s) 686
BfmI CTRYAG 1 cut(s) 95
BfuAI ACCTGC 1 cut(s) 253
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme1390I CCNGG 2 cut(s) 585, 595
Bme18I GGWCC 2 cut(s) 190, 591
BmgT120I GGNCC 2 cut(s) 190, 591
BmiI GGNNCC 1 cut(s) 582
BmrFI CCNGG 2 cut(s) 585, 595
BmsI GCATC 2 cut(s) 61, 382
Bpu14I TTCGAA 2 cut(s) 458, 524
BpuEI CTTGAG 1 cut(s) 181
BpuMI CCSGG 1 cut(s) 585
BsaI GGTCTC 1 cut(s) 648
BsaJI CCNNGG 1 cut(s) 561
BsaWI WCCGGW 1 cut(s) 344
Bse1I ACTGG 1 cut(s) 94
Bse3DI GCAATG 1 cut(s) 111
BseBI CCWGG 1 cut(s) 595
BseDI CCNNGG 1 cut(s) 561
BseGI GGATG 2 cut(s) 593, 646
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 1 cut(s) 593
BseXI GCAGC 1 cut(s) 109
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 1 cut(s) 132
BsiEI CGRYCG 1 cut(s) 108
BsiSI CCGG 2 cut(s) 345, 584
BsmAI GTCTC 1 cut(s) 648
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 1 cut(s) 132
Bso31I GGTCTC 1 cut(s) 648
Bsp119I TTCGAA 2 cut(s) 458, 524
Bsp143I GATC 3 cut(s) 105, 610, 637
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 1 cut(s) 308
BspHI TCATGA 1 cut(s) 432
BspLI GGNNCC 1 cut(s) 582
BspMAI CTGCAG 1 cut(s) 99
BspMI ACCTGC 1 cut(s) 253
BspPI GGATC 1 cut(s) 618
BspT104I TTCGAA 2 cut(s) 458, 524
BspTNI GGTCTC 1 cut(s) 648
BsrDI GCAATG 1 cut(s) 111
BsrI ACTGG 1 cut(s) 94
BssECI CCNNGG 1 cut(s) 561
BssMI GATC 3 cut(s) 105, 610, 637
BssT1I CCWWGG 1 cut(s) 561
Bst2UI CCWGG 1 cut(s) 595
BstBI TTCGAA 2 cut(s) 458, 524
BstDEI CTNAG 2 cut(s) 295, 359
BstF5I GGATG 2 cut(s) 593, 646
BstKTI GATC 3 cut(s) 108, 613, 640
BstMAI GTCTC 1 cut(s) 648
BstMBI GATC 3 cut(s) 105, 610, 637
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 2 cut(s) 460, 695
BstNI CCWGG 1 cut(s) 595
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 2 cut(s) 583, 593
BstSFI CTRYAG 1 cut(s) 95
BstV1I GCAGC 1 cut(s) 109
BsuRI GGCC 1 cut(s) 132
BtsCI GGATG 2 cut(s) 593, 646
BveI ACCTGC 1 cut(s) 253
CciI TCATGA 1 cut(s) 432
Cfr13I GGNCC 2 cut(s) 190, 591
Csp6I GTAC 3 cut(s) 242, 351, 444
CviAII CATG 4 cut(s) 80, 328, 433, 473
CviJI RGCY 6 cut(s) 127, 132, 321, 463, 583, 689
CviKI_1 RGCY 6 cut(s) 127, 132, 321, 463, 583, 689
CviQI GTAC 3 cut(s) 242, 351, 444
DdeI CTNAG 2 cut(s) 295, 359
DpnI GATC 3 cut(s) 107, 612, 639
DpnII GATC 3 cut(s) 105, 610, 637
DraIII CACNNNGTG 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 561
Eco31I GGTCTC 1 cut(s) 648
Eco47I GGWCC 2 cut(s) 190, 591
EcoRII CCWGG 1 cut(s) 593
EcoT14I CCWWGG 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 4 cut(s) 83, 331, 436, 476
FaiI YATR 8 cut(s) 41, 81, 138, 329, 399, 434, 474, 707
FatI CATG 4 cut(s) 79, 327, 432, 472
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 2 cut(s) 600, 633
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 1 cut(s) 686
GluI GCNGC 1 cut(s) 98
HaeIII GGCC 1 cut(s) 132
HapII CCGG 2 cut(s) 345, 584
Hin1II CATG 4 cut(s) 83, 331, 436, 476
HindIII AAGCTT 2 cut(s) 125, 461
HinfI GANTC 1 cut(s) 181
HpaII CCGG 2 cut(s) 345, 584
HphI GGTGA 2 cut(s) 74, 437
Hpy166II GTNNAC 3 cut(s) 87, 167, 353
Hpy188I TCNGA 5 cut(s) 186, 340, 510, 553, 714
Hpy188III TCNNGA 3 cut(s) 198, 433, 485
Hpy8I GTNNAC 3 cut(s) 87, 167, 353
HpyAV CCTTC 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 738
HpyCH4V TGCA 4 cut(s) 53, 97, 454, 623
HpyF10VI GCNNNNNNNGC 2 cut(s) 460, 695
HpyF3I CTNAG 2 cut(s) 295, 359
HpySE526I ACGT 1 cut(s) 738
Hsp92II CATG 4 cut(s) 83, 331, 436, 476
Kzo9I GATC 3 cut(s) 105, 610, 637
LmnI GCTCC 1 cut(s) 580
LpnPI CCDG 7 cut(s) 45, 75, 258, 358, 580, 597, 607
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 2 cut(s) 61, 382
MaeI CTAG 1 cut(s) 686
MaeII ACGT 1 cut(s) 738
MaeIII GTNAC 3 cut(s) 149, 288, 485
MalI GATC 3 cut(s) 107, 612, 639
MboI GATC 3 cut(s) 105, 610, 637
MboII GAAGA 5 cut(s) 233, 508, 713, 717, 755
MfeI CAATTG 1 cut(s) 624
MmeI TCCRAC 1 cut(s) 657
MnlI CCTC 5 cut(s) 143, 155, 341, 504, 571
MseI TTAA 4 cut(s) 14, 236, 479, 754
MspI CCGG 2 cut(s) 345, 584
MspR9I CCNGG 2 cut(s) 585, 595
MunI CAATTG 1 cut(s) 624
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 1 cut(s) 595
MwoI GCNNNNNNNGC 2 cut(s) 460, 695
NciI CCSGG 1 cut(s) 585
NdeII GATC 3 cut(s) 105, 610, 637
NlaIII CATG 4 cut(s) 83, 331, 436, 476
NlaIV GGNNCC 1 cut(s) 582
NmuCI GTSAC 3 cut(s) 149, 288, 485
NspI RCATGY 1 cut(s) 331
NspV TTCGAA 2 cut(s) 458, 524
PagI TCATGA 1 cut(s) 432
PctI GAATGC 1 cut(s) 454
PfeI GAWTC 1 cut(s) 181
PfoI TCCNGGA 1 cut(s) 593
PkrI GCNGC 1 cut(s) 99
Ple19I CGATCG 1 cut(s) 108
PsiI TTATAA 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 593
PspGI CCWGG 1 cut(s) 593
PspN4I GGNNCC 1 cut(s) 582
PspPI GGNCC 2 cut(s) 190, 591
PstI CTGCAG 1 cut(s) 99
PvuI CGATCG 1 cut(s) 108
RsaI GTAC 3 cut(s) 243, 352, 445
RsaNI GTAC 3 cut(s) 242, 351, 444
SaqAI TTAA 4 cut(s) 14, 236, 479, 754
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 3 cut(s) 105, 610, 637
Sau96I GGNCC 2 cut(s) 190, 591
ScrFI CCNGG 2 cut(s) 585, 595
SfaNI GCATC 2 cut(s) 61, 382
SfcI CTRYAG 1 cut(s) 95
SfuI TTCGAA 2 cut(s) 458, 524
SinI GGWCC 2 cut(s) 190, 591
SmlI CTYRAG 1 cut(s) 196
SmoI CTYRAG 1 cut(s) 196
SspMI CTAG 1 cut(s) 686
StyD4I CCNGG 2 cut(s) 583, 593
StyI CCWWGG 1 cut(s) 561
TaiI ACGT 1 cut(s) 741
TaqI TCGA 4 cut(s) 108, 458, 524, 613
TaqII GACCGA 1 cut(s) 630
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 4 cut(s) 14, 236, 479, 754
Tru9I TTAA 4 cut(s) 14, 236, 479, 754
TseFI GTSAC 3 cut(s) 149, 288, 485
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 3 cut(s) 149, 288, 485
TspDTI ATGAA 4 cut(s) 32, 133, 297, 449
VpaK11BI GGWCC 2 cut(s) 190, 591
XapI RAATTY 4 cut(s) 140, 211, 255, 407
XceI RCATGY 1 cut(s) 331
XcmI CCANNNNNNNNNTGG 2 cut(s) 86, 214
XspI CTAG 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.