MD01G1121000.v1.1

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0020384
Physical Location & Seq
Forward (+)
23466897 .. 23468098
1202 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1121000.v1.1.491

Sequence Viewer

Length: 723 bp
ATGGCAACCTCCAATCCAAGTGTTTGTTTGAAGCTCCTCATCGATACAAAGGGTCAACGAGTTCTCTTTGCTGAAGCTGGCCAAGACTTTGTGGACTTTCTCCTCACAATCCTGTCTTTGCCTCTTGGCACTGTCATCCGCCTCCTCTCCAAAGATGGCATGGTTGGCTCGTTAGGCAAGCTTTATGGCAGTGTCGAAAGCCTAAGCAGCACATACATGCAACCACACTTTAACAAGGAATCCCTTCTGAAACCTAAGGCAACTGCAACAAGTGATGTTGGTGCTGATGTCCTTCATATGCTGACGATAGATGACTCCTCTGCTGAAAAGTCAATCTATGGTTGTCGTAATTGTTGTTGTAATTACAGCAACCGGCCAATCGTTGTAACCGACGACCCTAAAGCCACTTGTCCGCACTGCCGTTCAAGCATAACTTCACCGGCGACTTTTGTCCATCGATCCGCTGCTGAAAGAACAACGTCCGGCGAGGGAGGGTATGTGAAAGGTGTTGTGACTTATATGATTATGGATGACCTGGAGGTGAAGCCTATGTCTACCATTTCAAGCGTAACCATGCTCAACACGTTCAACGTCAAGGATGTCGGTGCCCTTGAAGAGAAGGAGGTCCATCTTACCATGGAAGAGGGTGTCAAATTGCTGAGGGTATCATTGCTGTCAAACTTGGTTCTGACCACCGTGTTCCTTGATAAGAATGAAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

241

Amino Acids

26.02

Weight (kDa)

5.75

Isoelectric Point (pI)

27.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 5 - 238 3.6e-45 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020384)

Species Orthologous Gene IDs
malus_domestica MD01G1121000.v1.1 MD01G1121100.v1.1
pyrus_communis pycom01g14770 pycom01g14790 pycom01g14800 pycom01g14810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 605
AccI GTMKAC 1 cut(s) 554
AciI CCGC 3 cut(s) 139, 413, 462
AclWI GGATC 1 cut(s) 453
AcoI YGGCCR 2 cut(s) 79, 374
AcuI CTGAAG 1 cut(s) 93
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 5 cut(s) 31, 426, 564, 589, 614
AjnI CCWGG 1 cut(s) 534
AluBI AGCT 3 cut(s) 34, 77, 181
AluI AGCT 3 cut(s) 34, 77, 181
AlwI GGATC 1 cut(s) 453
AoxI GGCC 2 cut(s) 79, 374
ApeKI GCWGC 2 cut(s) 207, 464
Asp700I GAANNNNTTC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 625
AsuHPI GGTGA 2 cut(s) 429, 553
AvaII GGWCC 1 cut(s) 625
AxyI CCTNAGG 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 610
BalI TGGCCA 1 cut(s) 81
BanI GGYRCC 1 cut(s) 605
BbvCI CCTCAGC 1 cut(s) 659
BbvI GCAGC 2 cut(s) 219, 451
BccI CCATC 3 cut(s) 149, 462, 636
BceAI ACGGC 1 cut(s) 405
BciT130I CCWGG 1 cut(s) 536
BisI GCNGC 2 cut(s) 208, 465
BlsI GCNGC 2 cut(s) 209, 466
Bme1390I CCNGG 1 cut(s) 536
Bme18I GGWCC 1 cut(s) 625
BmgT120I GGNCC 1 cut(s) 625
BmiI GGNNCC 1 cut(s) 607
BmrFI CCNGG 1 cut(s) 536
BoxI GACNNNNGTC 1 cut(s) 449
BpmI CTGGAG 1 cut(s) 557
Bpu10I CCTNAGC 2 cut(s) 203, 659
Bsa29I ATCGAT 2 cut(s) 42, 457
BsaJI CCNNGG 1 cut(s) 636
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bse118I RCCGGY 2 cut(s) 372, 439
Bse21I CCTNAGG 1 cut(s) 255
Bse3DI GCAATG 1 cut(s) 668
BseBI CCWGG 1 cut(s) 536
BseCI ATCGAT 2 cut(s) 42, 457
BseDI CCNNGG 1 cut(s) 636
BseGI GGATG 3 cut(s) 135, 535, 604
BseMI GCAATG 1 cut(s) 668
BseMII CTCAG 1 cut(s) 650
BseRI GAGGAG 4 cut(s) 26, 92, 134, 307
BseSI GKGCMC 1 cut(s) 610
BseXI GCAGC 2 cut(s) 219, 451
BshFI GGCC 2 cut(s) 81, 376
BshNI GGYRCC 1 cut(s) 605
BshVI ATCGAT 2 cut(s) 42, 457
BsiSI CCGG 3 cut(s) 373, 440, 483
BsnI GGCC 2 cut(s) 81, 376
Bsp1286I GDGCHC 1 cut(s) 610
Bsp143I GATC 1 cut(s) 458
Bsp19I CCATGG 1 cut(s) 636
BspACI CCGC 3 cut(s) 139, 413, 462
BspANI GGCC 2 cut(s) 81, 376
BspCNI CTCAG 1 cut(s) 651
BspDI ATCGAT 2 cut(s) 42, 457
BspLI GGNNCC 1 cut(s) 607
BspPI GGATC 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 605
BsrDI GCAATG 1 cut(s) 668
BsrFI RCCGGY 2 cut(s) 372, 439
BssAI RCCGGY 2 cut(s) 372, 439
BssECI CCNNGG 1 cut(s) 636
BssMI GATC 1 cut(s) 458
BssT1I CCWWGG 1 cut(s) 636
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 2 cut(s) 133, 697
Bst6I CTCTTC 2 cut(s) 609, 636
BstC8I GCNNGC 2 cut(s) 79, 179
BstDEI CTNAG 3 cut(s) 203, 255, 659
BstDSI CCRYGG 1 cut(s) 636
BstF5I GGATG 3 cut(s) 135, 535, 604
BstKTI GATC 1 cut(s) 461
BstMBI GATC 1 cut(s) 458
BstMWI GCNNNNNNNGC 4 cut(s) 165, 174, 207, 426
BstNI CCWGG 1 cut(s) 536
BstNSI RCATGY 1 cut(s) 220
BstPAI GACNNNNGTC 1 cut(s) 449
BstSCI CCNGG 1 cut(s) 534
BstSLI GKGCMC 1 cut(s) 610
BstV1I GCAGC 2 cut(s) 219, 451
Bsu15I ATCGAT 2 cut(s) 42, 457
Bsu36I CCTNAGG 1 cut(s) 255
BsuRI GGCC 2 cut(s) 81, 376
BsuTUI ATCGAT 2 cut(s) 42, 457
BtgI CCRYGG 1 cut(s) 636
BtsCI GGATG 3 cut(s) 135, 535, 604
BtsI GCAGTG 2 cut(s) 196, 415
BtsIMutI CAGTG 3 cut(s) 129, 196, 415
Cac8I GCNNGC 2 cut(s) 79, 179
Cfr10I RCCGGY 2 cut(s) 372, 439
Cfr13I GGNCC 1 cut(s) 625
ClaI ATCGAT 2 cut(s) 42, 457
CviAII CATG 4 cut(s) 160, 217, 574, 637
CviJI RGCY 9 cut(s) 34, 77, 81, 168, 181, 201, 376, 404, 547
CviKI_1 RGCY 9 cut(s) 34, 77, 81, 168, 181, 201, 376, 404, 547
DdeI CTNAG 3 cut(s) 203, 255, 659
DpnI GATC 1 cut(s) 460
DpnII GATC 1 cut(s) 458
EaeI YGGCCR 2 cut(s) 79, 374
Eam1104I CTCTTC 2 cut(s) 609, 636
EarI CTCTTC 2 cut(s) 609, 636
EciI GGCGGA 1 cut(s) 128
Eco130I CCWWGG 1 cut(s) 636
Eco47I GGWCC 1 cut(s) 625
Eco57I CTGAAG 1 cut(s) 93
Eco81I CCTNAGG 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 534
EcoT14I CCWWGG 1 cut(s) 636
ErhI CCWWGG 1 cut(s) 636
FaeI CATG 4 cut(s) 163, 220, 577, 640
FalI AAGNNNNNCTT 2 cut(s) 418, 450
FatI CATG 4 cut(s) 159, 216, 573, 636
FauNDI CATATG 1 cut(s) 297
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 2 cut(s) 208, 465
FokI GGATG 3 cut(s) 122, 542, 611
Fsp4HI GCNGC 2 cut(s) 208, 465
GluI GCNGC 2 cut(s) 208, 465
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 2 cut(s) 81, 376
HapII CCGG 3 cut(s) 373, 440, 483
Hin1II CATG 4 cut(s) 163, 220, 577, 640
HincII GTYRAC 1 cut(s) 56
HindII GTYRAC 1 cut(s) 56
HindIII AAGCTT 1 cut(s) 179
HinfI GANTC 2 cut(s) 239, 314
HpaII CCGG 3 cut(s) 373, 440, 483
HphI GGTGA 2 cut(s) 429, 553
Hpy166II GTNNAC 3 cut(s) 56, 94, 555
Hpy188I TCNGA 2 cut(s) 249, 690
Hpy8I GTNNAC 3 cut(s) 56, 94, 555
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 3 cut(s) 254, 302, 613
HpyCH4III ACNGT 2 cut(s) 133, 697
HpyCH4IV ACGT 3 cut(s) 479, 584, 591
HpyCH4V TGCA 2 cut(s) 220, 266
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 174, 207, 426
HpyF3I CTNAG 3 cut(s) 203, 255, 659
HpySE526I ACGT 3 cut(s) 479, 584, 591
Hsp92II CATG 4 cut(s) 163, 220, 577, 640
Kzo9I GATC 1 cut(s) 458
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 7 cut(s) 63, 125, 386, 453, 496, 521, 548
Lsp1109I GCAGC 2 cut(s) 219, 451
MaeII ACGT 3 cut(s) 479, 584, 591
MaeIII GTNAC 3 cut(s) 385, 511, 568
MalI GATC 1 cut(s) 460
MboI GATC 1 cut(s) 458
MboII GAAGA 2 cut(s) 626, 653
MhlI GDGCHC 1 cut(s) 610
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 3 cut(s) 349, 361, 653
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 1 cut(s) 308
Mox20I TGGCCA 1 cut(s) 81
MroXI GAANNNNTTC 1 cut(s) 243
MscI TGGCCA 1 cut(s) 81
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 1 cut(s) 215
Msp20I TGGCCA 1 cut(s) 81
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 3 cut(s) 373, 440, 483
MspR9I CCNGG 1 cut(s) 536
MvaI CCWGG 1 cut(s) 536
MwoI GCNNNNNNNGC 4 cut(s) 165, 174, 207, 426
NcoI CCATGG 1 cut(s) 636
NdeI CATATG 1 cut(s) 297
NdeII GATC 1 cut(s) 458
NlaIII CATG 4 cut(s) 163, 220, 577, 640
NlaIV GGNNCC 1 cut(s) 607
NmuCI GTSAC 1 cut(s) 511
NspI RCATGY 1 cut(s) 220
PcsI WCGNNNNNNNCGW 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 243
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 2 cut(s) 209, 466
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
PshAI GACNNNNGTC 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 1 cut(s) 607
PspPI GGNCC 1 cut(s) 625
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 2 cut(s) 208, 465
Sau3AI GATC 1 cut(s) 458
Sau96I GGNCC 1 cut(s) 625
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 1 cut(s) 536
SduI GDGCHC 1 cut(s) 610
SgrAI CRCCGGYG 1 cut(s) 439
SinI GGWCC 1 cut(s) 625
SmiMI CAYNNNNRTG 1 cut(s) 215
Sse9I AATT 3 cut(s) 349, 361, 653
SsiI CCGC 3 cut(s) 139, 413, 462
StyD4I CCNGG 1 cut(s) 534
StyI CCWWGG 1 cut(s) 636
TaaI ACNGT 2 cut(s) 133, 697
TaiI ACGT 3 cut(s) 482, 587, 594
TaqI TCGA 3 cut(s) 42, 195, 457
TasI AATT 3 cut(s) 349, 361, 653
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 3 cut(s) 136, 196, 422
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 2 cut(s) 207, 464
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 1 cut(s) 284
TspRI CASTG 3 cut(s) 136, 196, 422
VpaK11BI GGWCC 1 cut(s) 625
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 157
XmiI GTMKAC 1 cut(s) 554
XmnI GAANNNNTTC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.