MD01G1121100.v1.1

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0020384
Physical Location & Seq
Forward (+)
23490811 .. 23492031
1221 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1121100.v1.1.491

Sequence Viewer

Length: 747 bp
ATGGAAAACCCCAAGCCAAGTATTTGCTTGAAGCTCCTTGTCGATACAAAGCGTCAACGAGTTCTCTTTGCTGAAGCTGGCAAAGACTTTGTGGACTTTCTCTTCACACTCCTGTCTTTGCCCATTGGCACTGTCATCGGGCTCCTTCCCAAAGATGGCATGGTTGGCTCGTTAGGCAAACTTTACGGCAGTGTCAAGAGCCTAAGCAGCACATACATGCAACCTTACTTTAACAAGGAATCCCTCCTGAAACCTAAGGCAACAGCAACGATTGGTGTTGGTGCCGATGTCCTTCATATGCTGATGATAGACGACTCCACTGCTGAAAAGTCATTCTATTATTGTGATTGTCGTAGTCGCGGCCAACAAAGGTACTGCTACAACGGATCCAGTGGAAATCCAACAGTTGTAACGGACAACCCTGAAGCAATTTGTCCACACTGCATTTCAAGCATAAATACCAAGGCGACTTTTGTCCATGGATCCGCTGCTGAAAGCAGAACGTCCGGCGAGGGAGGGTATGTGAAAGGTGTTGTCACGTATATGATTATGGATGACTTGGAAGTGCAGCCTATGTCTACCATTTCAGGCATAACCATGCTTAACAGGTTCAACGTCAGGGATGTCGGTGCCCTTGAAGAGAAGGTGGTCCATCTTACCATGGAAGAGGGTCTCAAATTGCTGAGGGCATCGTTGCACTCAAACTTGGTCCTGACCACCGTGTTCCTTGGTACGAAAGAAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

249

Amino Acids

27.04

Weight (kDa)

6.95

Isoelectric Point (pI)

25.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 5 - 246 1.2e-42 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020384)

Species Orthologous Gene IDs
malus_domestica MD01G1121000.v1.1 MD01G1121100.v1.1
pyrus_communis pycom01g14770 pycom01g14790 pycom01g14800 pycom01g14810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 281, 629
AccI GTMKAC 1 cut(s) 578
AccII CGCG 1 cut(s) 360
AciI CCGC 2 cut(s) 360, 486
AclWI GGATC 4 cut(s) 381, 394, 477, 490
AcoI YGGCCR 1 cut(s) 361
AcuI CTGAAG 2 cut(s) 93, 444
AfaI GTAC 2 cut(s) 374, 733
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 4 cut(s) 31, 450, 613, 638
AluBI AGCT 2 cut(s) 34, 77
AluI AGCT 2 cut(s) 34, 77
Alw26I GTCTC 1 cut(s) 677
AlwI GGATC 4 cut(s) 381, 394, 477, 490
AoxI GGCC 1 cut(s) 361
ApeKI GCWGC 3 cut(s) 207, 488, 568
AspS9I GGNCC 2 cut(s) 649, 709
AvaII GGWCC 2 cut(s) 649, 709
AxyI CCTNAGG 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 634
BamHI GGATCC 2 cut(s) 386, 482
BanI GGYRCC 2 cut(s) 281, 629
BanII GRGCYC 1 cut(s) 144
BbvCI CCTCAGC 1 cut(s) 683
BbvI GCAGC 3 cut(s) 219, 475, 580
BccI CCATC 2 cut(s) 149, 660
BceAI ACGGC 1 cut(s) 202
BcgI CGANNNNNNTGC 4 cut(s) 118, 152, 302, 336
BcoDI GTCTC 1 cut(s) 677
BisI GCNGC 4 cut(s) 208, 361, 489, 569
BlsI GCNGC 4 cut(s) 209, 362, 490, 570
Bme18I GGWCC 2 cut(s) 649, 709
BmgT120I GGNCC 2 cut(s) 649, 709
BmiI GGNNCC 5 cut(s) 143, 283, 388, 484, 631
BmsI GCATC 1 cut(s) 698
BoxI GACNNNNGTC 1 cut(s) 473
Bpu10I CCTNAGC 2 cut(s) 203, 683
BsaAI YACGTR 1 cut(s) 540
BsaI GGTCTC 1 cut(s) 677
BsaJI CCNNGG 4 cut(s) 462, 478, 660, 727
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 390
Bse21I CCTNAGG 1 cut(s) 255
BseDI CCNNGG 4 cut(s) 462, 478, 660, 727
BseGI GGATG 2 cut(s) 559, 628
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMII CTCAG 1 cut(s) 674
BseNI ACTGG 1 cut(s) 390
BseSI GKGCMC 1 cut(s) 634
BseXI GCAGC 3 cut(s) 219, 475, 580
BsgI GTGCAG 1 cut(s) 587
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 1 cut(s) 363
BshNI GGYRCC 2 cut(s) 281, 629
BsiSI CCGG 1 cut(s) 507
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 1 cut(s) 677
BsnI GGCC 1 cut(s) 363
Bso31I GGTCTC 1 cut(s) 677
Bsp1286I GDGCHC 2 cut(s) 144, 634
Bsp143I GATC 2 cut(s) 386, 482
Bsp19I CCATGG 2 cut(s) 478, 660
BspACI CCGC 2 cut(s) 360, 486
BspANI GGCC 1 cut(s) 363
BspCNI CTCAG 1 cut(s) 675
BspFNI CGCG 1 cut(s) 360
BspLI GGNNCC 5 cut(s) 143, 283, 388, 484, 631
BspPI GGATC 4 cut(s) 381, 394, 477, 490
BspT107I GGYRCC 2 cut(s) 281, 629
BspTNI GGTCTC 1 cut(s) 677
BsrI ACTGG 1 cut(s) 390
BssECI CCNNGG 4 cut(s) 462, 478, 660, 727
BssMI GATC 2 cut(s) 386, 482
BssT1I CCWWGG 4 cut(s) 462, 478, 660, 727
Bst4CI ACNGT 3 cut(s) 133, 406, 721
Bst6I CTCTTC 3 cut(s) 107, 633, 660
BstBAI YACGTR 1 cut(s) 540
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 3 cut(s) 203, 255, 683
BstDSI CCRYGG 2 cut(s) 478, 660
BstF5I GGATG 2 cut(s) 559, 628
BstFNI CGCG 1 cut(s) 360
BstKTI GATC 2 cut(s) 389, 485
BstMAI GTCTC 1 cut(s) 677
BstMBI GATC 2 cut(s) 386, 482
BstMWI GCNNNNNNNGC 4 cut(s) 165, 174, 207, 450
BstNSI RCATGY 1 cut(s) 220
BstPAI GACNNNNGTC 1 cut(s) 473
BstSLI GKGCMC 1 cut(s) 634
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 3 cut(s) 219, 475, 580
BstX2I RGATCY 2 cut(s) 386, 482
BstYI RGATCY 2 cut(s) 386, 482
Bsu36I CCTNAGG 1 cut(s) 255
BsuRI GGCC 1 cut(s) 363
BtgI CCRYGG 2 cut(s) 478, 660
BtsCI GGATG 2 cut(s) 559, 628
BtsI GCAGTG 3 cut(s) 196, 318, 439
BtsIMutI CAGTG 5 cut(s) 129, 196, 318, 397, 439
Cac8I GCNNGC 1 cut(s) 79
Cfr13I GGNCC 2 cut(s) 649, 709
CseI GACGC 1 cut(s) 41
Csp6I GTAC 2 cut(s) 373, 732
CviAII CATG 5 cut(s) 160, 217, 479, 598, 661
CviJI RGCY 8 cut(s) 16, 34, 77, 142, 168, 201, 363, 571
CviKI_1 RGCY 8 cut(s) 16, 34, 77, 142, 168, 201, 363, 571
CviQI GTAC 2 cut(s) 373, 732
DdeI CTNAG 3 cut(s) 203, 255, 683
DpnI GATC 2 cut(s) 388, 484
DpnII GATC 2 cut(s) 386, 482
EaeI YGGCCR 1 cut(s) 361
Eam1104I CTCTTC 3 cut(s) 107, 633, 660
EarI CTCTTC 3 cut(s) 107, 633, 660
Eco130I CCWWGG 4 cut(s) 462, 478, 660, 727
Eco24I GRGCYC 1 cut(s) 144
Eco31I GGTCTC 1 cut(s) 677
Eco47I GGWCC 2 cut(s) 649, 709
Eco57I CTGAAG 2 cut(s) 93, 444
Eco81I CCTNAGG 1 cut(s) 255
EcoT14I CCWWGG 4 cut(s) 462, 478, 660, 727
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 4 cut(s) 462, 478, 660, 727
FaeI CATG 5 cut(s) 163, 220, 482, 601, 664
FatI CATG 5 cut(s) 159, 216, 478, 597, 660
FauNDI CATATG 1 cut(s) 297
FblI GTMKAC 1 cut(s) 578
Fnu4HI GCNGC 4 cut(s) 208, 361, 489, 569
FokI GGATG 2 cut(s) 566, 635
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 4 cut(s) 208, 361, 489, 569
GluI GCNGC 4 cut(s) 208, 361, 489, 569
HaeIII GGCC 1 cut(s) 363
HapII CCGG 1 cut(s) 507
HgaI GACGC 1 cut(s) 41
Hin1II CATG 5 cut(s) 163, 220, 482, 601, 664
HincII GTYRAC 1 cut(s) 56
HindII GTYRAC 1 cut(s) 56
HinfI GANTC 2 cut(s) 239, 314
HpaII CCGG 1 cut(s) 507
Hpy166II GTNNAC 4 cut(s) 56, 94, 437, 579
Hpy188III TCNNGA 3 cut(s) 196, 247, 712
Hpy8I GTNNAC 4 cut(s) 56, 94, 437, 579
HpyAV CCTTC 3 cut(s) 155, 302, 637
HpyCH4III ACNGT 3 cut(s) 133, 406, 721
HpyCH4IV ACGT 3 cut(s) 503, 539, 615
HpyCH4V TGCA 4 cut(s) 220, 444, 568, 697
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 174, 207, 450
HpyF3I CTNAG 3 cut(s) 203, 255, 683
HpySE526I ACGT 3 cut(s) 503, 539, 615
Hsp92II CATG 5 cut(s) 163, 220, 482, 601, 664
Kzo9I GATC 2 cut(s) 386, 482
LmnI GCTCC 2 cut(s) 39, 147
Lsp1109I GCAGC 3 cut(s) 219, 475, 580
LweI GCATC 1 cut(s) 698
MaeII ACGT 3 cut(s) 503, 539, 615
MaeIII GTNAC 2 cut(s) 409, 535
MalI GATC 2 cut(s) 388, 484
MboI GATC 2 cut(s) 386, 482
MboII GAAGA 3 cut(s) 94, 650, 677
MflI RGATCY 2 cut(s) 386, 482
MhlI GDGCHC 2 cut(s) 144, 634
MluCI AATT 2 cut(s) 429, 677
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 425
MnlI CCTC 5 cut(s) 254, 505, 509, 661, 678
MseI TTAA 2 cut(s) 231, 603
MslI CAYNNNNRTG 3 cut(s) 215, 542, 596
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 1 cut(s) 507
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 4 cut(s) 165, 174, 207, 450
NcoI CCATGG 2 cut(s) 478, 660
NdeI CATATG 1 cut(s) 297
NdeII GATC 2 cut(s) 386, 482
NlaIII CATG 5 cut(s) 163, 220, 482, 601, 664
NlaIV GGNNCC 5 cut(s) 143, 283, 388, 484, 631
NmuCI GTSAC 1 cut(s) 535
NspI RCATGY 1 cut(s) 220
PcsI WCGNNNNNNNCGW 1 cut(s) 740
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 4 cut(s) 209, 362, 490, 570
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 540
PshAI GACNNNNGTC 1 cut(s) 473
PspN4I GGNNCC 5 cut(s) 143, 283, 388, 484, 631
PspPI GGNCC 2 cut(s) 649, 709
PsuI RGATCY 2 cut(s) 386, 482
RsaI GTAC 2 cut(s) 374, 733
RsaNI GTAC 2 cut(s) 373, 732
RseI CAYNNNNRTG 3 cut(s) 215, 542, 596
SaqAI TTAA 2 cut(s) 231, 603
SatI GCNGC 4 cut(s) 208, 361, 489, 569
Sau3AI GATC 2 cut(s) 386, 482
Sau96I GGNCC 2 cut(s) 649, 709
SchI GAGTC 1 cut(s) 308
SduI GDGCHC 2 cut(s) 144, 634
SfaNI GCATC 1 cut(s) 698
SinI GGWCC 2 cut(s) 649, 709
SmiMI CAYNNNNRTG 3 cut(s) 215, 542, 596
Sse9I AATT 2 cut(s) 429, 677
SsiI CCGC 2 cut(s) 360, 486
StyI CCWWGG 4 cut(s) 462, 478, 660, 727
TaaI ACNGT 3 cut(s) 133, 406, 721
TaiI ACGT 3 cut(s) 506, 542, 618
TaqI TCGA 1 cut(s) 42
TasI AATT 2 cut(s) 429, 677
TauI GCSGC 1 cut(s) 363
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 2 cut(s) 231, 603
Tru9I TTAA 2 cut(s) 231, 603
TscAI CASTG 5 cut(s) 136, 196, 325, 397, 446
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 3 cut(s) 207, 488, 568
Tsp45I GTSAC 1 cut(s) 535
TspDTI ATGAA 1 cut(s) 284
TspGWI ACGGA 2 cut(s) 399, 428
TspRI CASTG 5 cut(s) 136, 196, 325, 397, 446
VpaK11BI GGWCC 2 cut(s) 649, 709
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 157
XmiI GTMKAC 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.