pycom01g14770

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0020384
Physical Location & Seq
Forward (+)
14845830 .. 14846710
881 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g14770.1

Sequence Viewer

Length: 774 bp
ATGGAAACCTCCAAGCCAAGTATTTGTTTGAAGCTCCTTATTGACACAACGCGTCAACGAGTTCTCTATGCTGAAGCTGGCAAAGACTTTGTGGACTTTCTCCTCACAATTCTGTCTTTGCCGATTGGCACTGTCATCCGGCTTCTCTCCAAAGATGGCATGGTTGGCTCGTTAGGCAAACTTTACGGCAGTGTCGAAAGCCTAAGCAGCATATACATGCAACCTCACTTTAACAAGGAATCCCTCCTGAAACCTAACGCAACATCAACGATTGGTGTTGGTGCCGATGTCCTTCATATGCTGACGATAGACGACTCCTCTGCTGAAAAGTCATTCTATTATTGTGGTTGTCGTAGTCGCCACCAAACCTATCCAACCCTTGTAATGGACAACCCTAAAGCCACCGTTGTAACGGACAACCCTAAAGCCATTTGTCCATACTGCAGTTCAAGCATAAATACCAAGGCGACTTTTGTCCATGGATCCGCTGCTGAAGGAGAAACGGCCGGCGAGGGAGGATATGTGAAAGGTGTTGTGACGTATATGATTATGGATGACCTGGAAGTGAGGCCCATGTCTAGCATTTCAAGCATAACCATGCTAAACAGGTTCAACGTCAAGGATGTCGGTGCCCTTGAAGAGAAAGTGGTCCATCTTACCATGGAAGAGGGTGTCAAATTGCTGAGGGTATCGTTGCTGTCAAACTTGGTTCTGACCACCGTATTCCTTGATAAGAATGAAGCGTGCACTTCAACTGAAAGTATTGTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

258

Amino Acids

28.0

Weight (kDa)

5.6

Isoelectric Point (pI)

31.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 5 - 253 5.3e-43 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020384)

Species Orthologous Gene IDs
malus_domestica MD01G1121000.v1.1 MD01G1121100.v1.1
pyrus_communis pycom01g14770 pycom01g14790 pycom01g14800 pycom01g14810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 281, 629
AccII CGCG 1 cut(s) 52
AciI CCGC 1 cut(s) 486
AclWI GGATC 2 cut(s) 477, 490
AcoI YGGCCR 1 cut(s) 504
AcuI CTGAAG 2 cut(s) 93, 513
AfiI CCNNNNNNNGG 1 cut(s) 385
AflIII ACRYGT 1 cut(s) 50
AgsI TTSAA 6 cut(s) 31, 450, 588, 613, 638, 753
AjnI CCWGG 1 cut(s) 558
AluBI AGCT 2 cut(s) 34, 77
AluI AGCT 2 cut(s) 34, 77
Alw21I GWGCWC 1 cut(s) 749
Alw44I GTGCAC 1 cut(s) 745
AlwI GGATC 2 cut(s) 477, 490
AoxI GGCC 2 cut(s) 504, 569
ApaLI GTGCAC 1 cut(s) 745
ApeKI GCWGC 2 cut(s) 207, 488
AspS9I GGNCC 2 cut(s) 570, 649
AvaII GGWCC 1 cut(s) 649
BaeGI GKGCMC 2 cut(s) 634, 749
BamHI GGATCC 1 cut(s) 482
BanI GGYRCC 2 cut(s) 281, 629
Bbv12I GWGCWC 1 cut(s) 749
BbvCI CCTCAGC 1 cut(s) 683
BbvI GCAGC 2 cut(s) 219, 475
BccI CCATC 2 cut(s) 149, 660
BceAI ACGGC 2 cut(s) 202, 519
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 1 cut(s) 560
BfaI CTAG 1 cut(s) 579
BfmI CTRYAG 1 cut(s) 442
BisI GCNGC 2 cut(s) 208, 489
BlsI GCNGC 2 cut(s) 209, 490
Bme1390I CCNGG 1 cut(s) 560
Bme18I GGWCC 1 cut(s) 649
BmgT120I GGNCC 2 cut(s) 570, 649
BmiI GGNNCC 3 cut(s) 283, 484, 631
BmrFI CCNGG 1 cut(s) 560
BoxI GACNNNNGTC 1 cut(s) 473
Bpu10I CCTNAGC 2 cut(s) 203, 683
BsaJI CCNNGG 3 cut(s) 462, 478, 660
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 1 cut(s) 385
Bse118I RCCGGY 1 cut(s) 506
BseBI CCWGG 1 cut(s) 560
BseDI CCNNGG 3 cut(s) 462, 478, 660
BseGI GGATG 3 cut(s) 135, 559, 628
BseLI CCNNNNNNNGG 1 cut(s) 385
BseMII CTCAG 1 cut(s) 674
BseRI GAGGAG 2 cut(s) 92, 307
BseSI GKGCMC 2 cut(s) 634, 749
BseX3I CGGCCG 1 cut(s) 504
BseXI GCAGC 2 cut(s) 219, 475
Bsh1236I CGCG 1 cut(s) 52
Bsh1285I CGRYCG 1 cut(s) 507
BshFI GGCC 2 cut(s) 506, 571
BshNI GGYRCC 2 cut(s) 281, 629
BsiEI CGRYCG 1 cut(s) 507
BsiHKAI GWGCWC 1 cut(s) 749
BsiSI CCGG 2 cut(s) 139, 507
BslI CCNNNNNNNGG 1 cut(s) 385
BsnI GGCC 2 cut(s) 506, 571
Bsp1286I GDGCHC 2 cut(s) 634, 749
Bsp143I GATC 1 cut(s) 482
Bsp19I CCATGG 2 cut(s) 478, 660
BspACI CCGC 1 cut(s) 486
BspANI GGCC 2 cut(s) 506, 571
BspCNI CTCAG 1 cut(s) 675
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 3 cut(s) 283, 484, 631
BspMAI CTGCAG 1 cut(s) 446
BspPI GGATC 2 cut(s) 477, 490
BspT107I GGYRCC 2 cut(s) 281, 629
BsrFI RCCGGY 1 cut(s) 506
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 3 cut(s) 462, 478, 660
BssMI GATC 1 cut(s) 482
BssT1I CCWWGG 3 cut(s) 462, 478, 660
Bst2UI CCWGG 1 cut(s) 560
Bst4CI ACNGT 3 cut(s) 133, 406, 721
Bst6I CTCTTC 2 cut(s) 633, 660
BstC8I GCNNGC 3 cut(s) 79, 508, 745
BstDEI CTNAG 2 cut(s) 203, 683
BstDSI CCRYGG 2 cut(s) 478, 660
BstF5I GGATG 3 cut(s) 135, 559, 628
BstFNI CGCG 1 cut(s) 52
BstKTI GATC 1 cut(s) 485
BstMBI GATC 1 cut(s) 482
BstMCI CGRYCG 1 cut(s) 507
BstMWI GCNNNNNNNGC 5 cut(s) 165, 174, 207, 450, 588
BstNI CCWGG 1 cut(s) 560
BstNSI RCATGY 1 cut(s) 220
BstPAI GACNNNNGTC 1 cut(s) 473
BstSCI CCNGG 1 cut(s) 558
BstSFI CTRYAG 1 cut(s) 442
BstSLI GKGCMC 2 cut(s) 634, 749
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 2 cut(s) 219, 475
BstX2I RGATCY 1 cut(s) 482
BstYI RGATCY 1 cut(s) 482
BstZI CGGCCG 1 cut(s) 504
BsuRI GGCC 2 cut(s) 506, 571
BtgI CCRYGG 2 cut(s) 478, 660
BtsCI GGATG 3 cut(s) 135, 559, 628
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 2 cut(s) 129, 196
Cac8I GCNNGC 3 cut(s) 79, 508, 745
Cfr10I RCCGGY 1 cut(s) 506
Cfr13I GGNCC 2 cut(s) 570, 649
CseI GACGC 1 cut(s) 41
CviAII CATG 6 cut(s) 160, 217, 479, 574, 598, 661
DdeI CTNAG 2 cut(s) 203, 683
DpnI GATC 1 cut(s) 484
DpnII GATC 1 cut(s) 482
EaeI YGGCCR 1 cut(s) 504
EagI CGGCCG 1 cut(s) 504
Eam1104I CTCTTC 2 cut(s) 633, 660
EarI CTCTTC 2 cut(s) 633, 660
EclXI CGGCCG 1 cut(s) 504
Eco130I CCWWGG 3 cut(s) 462, 478, 660
Eco47I GGWCC 1 cut(s) 649
Eco52I CGGCCG 1 cut(s) 504
Eco57I CTGAAG 2 cut(s) 93, 513
EcoRII CCWGG 1 cut(s) 558
EcoT14I CCWWGG 3 cut(s) 462, 478, 660
ErhI CCWWGG 3 cut(s) 462, 478, 660
FaeI CATG 6 cut(s) 163, 220, 482, 577, 601, 664
FatI CATG 6 cut(s) 159, 216, 478, 573, 597, 660
FauNDI CATATG 1 cut(s) 297
Fnu4HI GCNGC 2 cut(s) 208, 489
FokI GGATG 3 cut(s) 122, 566, 635
Fsp4HI GCNGC 2 cut(s) 208, 489
FspBI CTAG 1 cut(s) 579
GluI GCNGC 2 cut(s) 208, 489
HaeIII GGCC 2 cut(s) 506, 571
HapII CCGG 2 cut(s) 139, 507
HgaI GACGC 1 cut(s) 41
Hin1II CATG 6 cut(s) 163, 220, 482, 577, 601, 664
HincII GTYRAC 1 cut(s) 56
HindII GTYRAC 1 cut(s) 56
HinfI GANTC 2 cut(s) 239, 314
HpaII CCGG 2 cut(s) 139, 507
Hpy166II GTNNAC 3 cut(s) 56, 94, 747
Hpy188I TCNGA 1 cut(s) 714
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 3 cut(s) 56, 94, 747
HpyAV CCTTC 2 cut(s) 302, 488
HpyCH4III ACNGT 3 cut(s) 133, 406, 721
HpyCH4IV ACGT 2 cut(s) 539, 615
HpyCH4V TGCA 3 cut(s) 220, 444, 747
HpyF10VI GCNNNNNNNGC 5 cut(s) 165, 174, 207, 450, 588
HpyF3I CTNAG 2 cut(s) 203, 683
HpySE526I ACGT 2 cut(s) 539, 615
Hsp92II CATG 6 cut(s) 163, 220, 482, 577, 601, 664
KroI GCCGGC 1 cut(s) 506
KroNI GCCGGC 1 cut(s) 508
Kzo9I GATC 1 cut(s) 482
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 7 cut(s) 63, 152, 260, 520, 545, 572, 592
Lsp1109I GCAGC 2 cut(s) 219, 475
MaeI CTAG 1 cut(s) 579
MaeII ACGT 2 cut(s) 539, 615
MaeIII GTNAC 2 cut(s) 409, 535
MalI GATC 1 cut(s) 484
MboI GATC 1 cut(s) 482
MboII GAAGA 2 cut(s) 650, 677
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 2 cut(s) 634, 749
MluCI AATT 2 cut(s) 108, 677
MluI ACGCGT 1 cut(s) 50
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 398
MroNI GCCGGC 1 cut(s) 506
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 2 cut(s) 215, 596
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 2 cut(s) 139, 507
MspR9I CCNGG 1 cut(s) 560
MvaI CCWGG 1 cut(s) 560
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 5 cut(s) 165, 174, 207, 450, 588
NaeI GCCGGC 1 cut(s) 508
NcoI CCATGG 2 cut(s) 478, 660
NdeI CATATG 1 cut(s) 297
NdeII GATC 1 cut(s) 482
NgoMIV GCCGGC 1 cut(s) 506
NlaIII CATG 6 cut(s) 163, 220, 482, 577, 601, 664
NlaIV GGNNCC 3 cut(s) 283, 484, 631
NmuCI GTSAC 1 cut(s) 535
NspI RCATGY 1 cut(s) 220
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PdiI GCCGGC 1 cut(s) 508
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 2 cut(s) 209, 490
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
PshAI GACNNNNGTC 1 cut(s) 473
Psp6I CCWGG 1 cut(s) 558
PspGI CCWGG 1 cut(s) 558
PspN4I GGNNCC 3 cut(s) 283, 484, 631
PspPI GGNCC 2 cut(s) 570, 649
PstI CTGCAG 1 cut(s) 446
PsuI RGATCY 1 cut(s) 482
RseI CAYNNNNRTG 2 cut(s) 215, 596
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 2 cut(s) 208, 489
Sau3AI GATC 1 cut(s) 482
Sau96I GGNCC 2 cut(s) 570, 649
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 1 cut(s) 560
SduI GDGCHC 2 cut(s) 634, 749
SfcI CTRYAG 1 cut(s) 442
SinI GGWCC 1 cut(s) 649
SmiMI CAYNNNNRTG 2 cut(s) 215, 596
Sse9I AATT 2 cut(s) 108, 677
SsiI CCGC 1 cut(s) 486
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 558
StyI CCWWGG 3 cut(s) 462, 478, 660
TaaI ACNGT 3 cut(s) 133, 406, 721
TaiI ACGT 2 cut(s) 542, 618
TaqI TCGA 1 cut(s) 195
TasI AATT 2 cut(s) 108, 677
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 2 cut(s) 136, 196
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 2 cut(s) 207, 488
Tsp45I GTSAC 1 cut(s) 535
TspDTI ATGAA 2 cut(s) 284, 753
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 2 cut(s) 136, 196
VneI GTGCAC 1 cut(s) 745
VpaK11BI GGWCC 1 cut(s) 649
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 157
XspI CTAG 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.