Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0021860

Physical Location & Seq

Genomic Coordinates Forward (+)

24352469 .. 24355460

Gene Structure 2992 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1133700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1083 bp

ATGGTCAAGGCTGGATTTGCTGGAGATGATGCTCCACGAGCTGTGTTCCCTAGCATTGTAGGGCGCCCTCGTCACACTGGTGTCATGGTTGGCATGGGCCAGAAAGATGCTTATGTTGGTGATGAGGCTCAATCCAAGCGTGGTATATTAACTTTGAAATACCCTATTGAGCATGGTATTGTGAGCAACTGGGATGACATGGAGAAGATTTGGCATCACACCTTCTATAATGAGCTTCGGGTTGCCCCAGAAGAGCACCCGGTCCTTCTAACTGAGGCCCCTCTGAATCCCAAAGCCAATCGTGAGAAGATGACCCAAATAATGTTTGAGACCTTTAACACTCCGGCTATGTATGTAGCCATTCAAGCTGTTCTTTCCCTGTATGCAAGTGGTCGTACAACTGGTATTGTGCTCGACTCTGGTGATGGTGTCAGCCATACGGTTCCCATTTACGAGGGGTATGCCCTCCCCCATGCCATCCTTCGTCTTGACCTTGCTGGTCGTGACCTCACTGATAGCTTGATGAAAATTCTTACGGAGCGTGGATATTCCTTCACGACCACTGCAGAGCGTGAAATTGTGAGGGACATGAAGGAAAAACTCTCTTACATTGCCCTTGACTATGAGCAGGAGATGGAGACTTCCAAAACCAGCTCTTCAGTTGAGAAGAGCTATGAGTTACCAGATGGGCAGGTCATTACCATTGGTGCAGAGCGGTTCCGATGCCCAGAAGTCCTTTTCCAACCATCAATGATCGGGATGGAAGCAGCAGGCATTCACGAAACCACGTACAACTCCATCATGAAGTGTGATGTTGATATCAGGAAAGACCTGTATGGCAACATTGTACTCTCTGGTGGTTCAACCATGTTCCCAGGCATTGCTGATAGAATGAGCAAGGAAATCACTGCCCTAGCCCCAAGCAGTATGAAGATCAAGGTGGTGGCACCACCCGAGAGGAAATACAGTGTCTGGATAGGAGGCTCCATCTTGGCTTCCCTTAGTACCTTCCAGCAGATGTGGATTGCAAAAGCAGAGTACGATGAGTCTGGGCCGTCTATTGTCCATAGGAAATGCTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005200 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005856 GO:0005886 GO:0005911 GO:0006996 GO:0007010 GO:0008150 GO:0009506 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0009987 GO:0016020 GO:0016043 GO:0030054 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Actin

Protein Analysis

361

Amino Acids

40.01

Weight (kDa)

5.67

Isoelectric Point (pI)

35.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Actin	PF00022	1 - 360	1.7e-141	Actin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0021860)

Species	Orthologous Gene IDs
malus_domestica	MD01G1133700.v1.1 MD07G1198000.v1.1
pyrus_communis	pycom01g15450 pycom07g18520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	682
AccB1I	GGYRCC	2 cut(s)	63, 946
AccBSI	CCGCTC	1 cut(s)	715
AciI	CCGC	1 cut(s)	715
AcsI	RAATTY	1 cut(s)	528
AcuI	CTGAAG	1 cut(s)	642
AcyI	GRCGYC	1 cut(s)	64
AfaI	GTAC	5 cut(s)	397, 791, 849, 1006, 1040
AgsI	TTSAA	3 cut(s)	157, 365, 864
AjnI	CCWGG	1 cut(s)	874
AleI	CACNNNNGTG	1 cut(s)	78
AluBI	AGCT	6 cut(s)	41, 235, 368, 519, 654, 672
AluI	AGCT	6 cut(s)	41, 235, 368, 519, 654, 672
Alw21I	GWGCWC	2 cut(s)	258, 414
Alw26I	GTCTC	2 cut(s)	323, 632
AlwNI	CAGNNNCTG	1 cut(s)	972
Ama87I	CYCGRG	1 cut(s)	953
AoxI	GGCC	3 cut(s)	97, 276, 1052
ApeKI	GCWGC	1 cut(s)	767
ApoI	RAATTY	1 cut(s)	528
Asp700I	GAANNNNTTC	1 cut(s)	1076
AspLEI	GCGC	1 cut(s)	66
AspS9I	GGNCC	4 cut(s)	97, 262, 277, 1052
AsuC2I	CCSGG	1 cut(s)	260
AsuHPI	GGTGA	2 cut(s)	131, 434
AvaI	CYCGRG	1 cut(s)	953
AvaII	GGWCC	1 cut(s)	262
BanI	GGYRCC	2 cut(s)	63, 946
BauI	CACGAG	1 cut(s)	36
Bbv12I	GWGCWC	2 cut(s)	258, 414
BbvI	GCAGC	1 cut(s)	779
BccI	CCATC	8 cut(s)	419, 485, 628, 680, 754, 754, 806, 995
BceAI	ACGGC	1 cut(s)	1039
BcgI	CGANNNNNNTGC	2 cut(s)	443, 477
BciT130I	CCWGG	1 cut(s)	876
BcnI	CCSGG	1 cut(s)	260
BcoDI	GTCTC	2 cut(s)	323, 632
BfaI	CTAG	2 cut(s)	51, 914
BfmI	CTRYAG	1 cut(s)	564
BfoI	RGCGCY	1 cut(s)	67
BfuAI	ACCTGC	1 cut(s)	682
BisI	GCNGC	1 cut(s)	768
BlsI	GCNGC	1 cut(s)	769
Bme1390I	CCNGG	2 cut(s)	260, 876
Bme18I	GGWCC	1 cut(s)	262
BmeT110I	CYCGRG	1 cut(s)	953
BmgT120I	GGNCC	4 cut(s)	97, 262, 277, 1052
BmiI	GGNNCC	6 cut(s)	65, 279, 444, 719, 948, 985
BmrFI	CCNGG	2 cut(s)	260, 876
BmrI	ACTGGG	1 cut(s)	199
BmsI	GCATC	4 cut(s)	19, 97, 223, 713
BmuI	ACTGGG	1 cut(s)	199
BpmI	CTGGAG	1 cut(s)	42
BpuMI	CCSGG	1 cut(s)	260
BsaAI	YACGTR	1 cut(s)	789
BsaHI	GRCGYC	1 cut(s)	64
BsaI	GGTCTC	1 cut(s)	323
BsaJI	CCNNGG	1 cut(s)	874
Bse1I	ACTGG	3 cut(s)	82, 194, 406
Bse3DI	GCAATG	2 cut(s)	609, 879
BseBI	CCWGG	1 cut(s)	876
BseDI	CCNNGG	1 cut(s)	874
BseGI	GGATG	3 cut(s)	199, 477, 765
BseMI	GCAATG	2 cut(s)	609, 879
BseMII	CTCAG	1 cut(s)	264
BseNI	ACTGG	3 cut(s)	82, 194, 406
BseXI	GCAGC	1 cut(s)	779
BsgI	GTGCAG	1 cut(s)	729
BshFI	GGCC	3 cut(s)	99, 278, 1054
BshNI	GGYRCC	2 cut(s)	63, 946
BsiHKAI	GWGCWC	2 cut(s)	258, 414
BsiHKCI	CYCGRG	1 cut(s)	953
BsiSI	CCGG	2 cut(s)	260, 344
BslFI	GGGAC	1 cut(s)	599
BsmAI	GTCTC	2 cut(s)	323, 632
BsmFI	GGGAC	1 cut(s)	599
BsmI	GAATGC	1 cut(s)	774
BsnI	GGCC	3 cut(s)	99, 278, 1054
Bso31I	GGTCTC	1 cut(s)	323
BsoBI	CYCGRG	1 cut(s)	953
Bsp1286I	GDGCHC	2 cut(s)	258, 414
Bsp143I	GATC	2 cut(s)	753, 933
BspACI	CCGC	1 cut(s)	715
BspANI	GGCC	3 cut(s)	99, 278, 1054
BspCNI	CTCAG	1 cut(s)	265
BspHI	TCATGA	1 cut(s)	801
BspLI	GGNNCC	6 cut(s)	65, 279, 444, 719, 948, 985
BspMAI	CTGCAG	1 cut(s)	568
BspMI	ACCTGC	1 cut(s)	682
BspQI	GCTCTTC	3 cut(s)	246, 661, 662
BspT107I	GGYRCC	2 cut(s)	63, 946
BspTNI	GGTCTC	1 cut(s)	323
BsrBI	CCGCTC	1 cut(s)	715
BsrDI	GCAATG	2 cut(s)	609, 879
BsrI	ACTGG	3 cut(s)	82, 194, 406
BssECI	CCNNGG	1 cut(s)	874
BssMI	GATC	2 cut(s)	753, 933
BssNI	GRCGYC	1 cut(s)	64
BssSI	CACGAG	1 cut(s)	36
Bst2BI	CACGAG	1 cut(s)	36
Bst2UI	CCWGG	1 cut(s)	876
Bst4CI	ACNGT	2 cut(s)	442, 968
Bst6I	CTCTTC	3 cut(s)	246, 661, 662
BstACI	GRCGYC	1 cut(s)	64
BstBAI	YACGTR	1 cut(s)	789
BstC8I	GCNNGC	1 cut(s)	772
BstDEI	CTNAG	2 cut(s)	273, 1001
BstF5I	GGATG	3 cut(s)	199, 477, 765
BstH2I	RGCGCY	1 cut(s)	67
BstHHI	GCGC	1 cut(s)	66
BstKTI	GATC	2 cut(s)	756, 936
BstMAI	GTCTC	2 cut(s)	323, 632
BstMBI	GATC	2 cut(s)	753, 933
BstMWI	GCNNNNNNNGC	3 cut(s)	17, 38, 365
BstNI	CCWGG	1 cut(s)	876
BstSCI	CCNGG	2 cut(s)	258, 874
BstSFI	CTRYAG	1 cut(s)	564
BstV1I	GCAGC	1 cut(s)	779
BsuRI	GGCC	3 cut(s)	99, 278, 1054
BtsCI	GGATG	3 cut(s)	199, 477, 765
BtsI	GCAGTG	2 cut(s)	561, 906
BtsIMutI	CAGTG	5 cut(s)	75, 510, 561, 906, 973
BveI	ACCTGC	1 cut(s)	682
Cac8I	GCNNGC	1 cut(s)	772
CaiI	CAGNNNCTG	1 cut(s)	972
CciI	TCATGA	1 cut(s)	801
CfoI	GCGC	1 cut(s)	66
Cfr13I	GGNCC	4 cut(s)	97, 262, 277, 1052
Csp6I	GTAC	5 cut(s)	396, 790, 848, 1005, 1039
CviAII	CATG	8 cut(s)	85, 94, 173, 199, 473, 589, 802, 868
CviQI	GTAC	5 cut(s)	396, 790, 848, 1005, 1039
DdeI	CTNAG	2 cut(s)	273, 1001
DinI	GGCGCC	1 cut(s)	65
DpnI	GATC	2 cut(s)	755, 935
DpnII	GATC	2 cut(s)	753, 933
Eam1104I	CTCTTC	3 cut(s)	246, 661, 662
EarI	CTCTTC	3 cut(s)	246, 661, 662
Eco31I	GGTCTC	1 cut(s)	323
Eco32I	GATATC	1 cut(s)	820
Eco47I	GGWCC	1 cut(s)	262
Eco57I	CTGAAG	1 cut(s)	642
Eco88I	CYCGRG	1 cut(s)	953
EcoO109I	RGGNCCY	1 cut(s)	277
EcoRII	CCWGG	1 cut(s)	874
EcoRV	GATATC	1 cut(s)	820
EgeI	GGCGCC	1 cut(s)	65
EheI	GGCGCC	1 cut(s)	65
FaeI	CATG	8 cut(s)	88, 97, 176, 202, 476, 592, 805, 871
FalI	AAGNNNNNCTT	2 cut(s)	357, 389
FaqI	GGGAC	1 cut(s)	599
FatI	CATG	8 cut(s)	84, 93, 172, 198, 472, 588, 801, 867
Fnu4HI	GCNGC	1 cut(s)	768
FokI	GGATG	3 cut(s)	206, 464, 772
Fsp4HI	GCNGC	1 cut(s)	768
FspBI	CTAG	2 cut(s)	51, 914
GlaI	GCGC	1 cut(s)	65
GluI	GCNGC	1 cut(s)	768
GsuI	CTGGAG	1 cut(s)	42
HaeII	RGCGCY	1 cut(s)	67
HaeIII	GGCC	3 cut(s)	99, 278, 1054
HapII	CCGG	2 cut(s)	260, 344
HhaI	GCGC	1 cut(s)	66
Hin1I	GRCGYC	1 cut(s)	64
Hin1II	CATG	8 cut(s)	88, 97, 176, 202, 476, 592, 805, 871
Hin6I	GCGC	1 cut(s)	64
HinP1I	GCGC	1 cut(s)	64
HinfI	GANTC	3 cut(s)	286, 416, 1046
HpaII	CCGG	2 cut(s)	260, 344
HphI	GGTGA	2 cut(s)	131, 434
Hpy188I	TCNGA	2 cut(s)	285, 722
Hpy188III	TCNNGA	9 cut(s)	302, 488, 503, 556, 757, 779, 802, 823, 973
HpyAV	CCTTC	6 cut(s)	232, 275, 491, 562, 586, 1018
HpyCH4III	ACNGT	2 cut(s)	442, 968
HpyCH4IV	ACGT	1 cut(s)	788
HpyCH4V	TGCA	4 cut(s)	386, 566, 710, 1028
HpyF10VI	GCNNNNNNNGC	3 cut(s)	17, 38, 365
HpyF3I	CTNAG	2 cut(s)	273, 1001
HpySE526I	ACGT	1 cut(s)	788
Hsp92I	GRCGYC	1 cut(s)	64
Hsp92II	CATG	8 cut(s)	88, 97, 176, 202, 476, 592, 805, 871
HspAI	GCGC	1 cut(s)	64
KasI	GGCGCC	1 cut(s)	63
Kzo9I	GATC	2 cut(s)	753, 933
LguI	GCTCTTC	3 cut(s)	246, 661, 662
LmnI	GCTCC	3 cut(s)	37, 538, 989
Lsp1109I	GCAGC	1 cut(s)	779
LweI	GCATC	4 cut(s)	19, 97, 223, 713
MaeI	CTAG	2 cut(s)	51, 914
MaeII	ACGT	1 cut(s)	788
MaeIII	GTNAC	3 cut(s)	71, 503, 678
MalI	GATC	2 cut(s)	755, 935
MbiI	CCGCTC	1 cut(s)	715
MboI	GATC	2 cut(s)	753, 933
MboII	GAAGA	6 cut(s)	217, 263, 319, 648, 679, 943
MhlI	GDGCHC	2 cut(s)	258, 414
MluCI	AATT	2 cut(s)	528, 576
Mly113I	GGCGCC	1 cut(s)	64
MlyI	GAGTC	2 cut(s)	410, 1055
MmeI	TCCRAC	1 cut(s)	766
MroXI	GAANNNNTTC	1 cut(s)	1076
MseI	TTAA	2 cut(s)	149, 336
MslI	CAYNNNNRTG	1 cut(s)	78
MspI	CCGG	2 cut(s)	260, 344
MspR9I	CCNGG	2 cut(s)	260, 876
Mva1269I	GAATGC	1 cut(s)	774
MvaI	CCWGG	1 cut(s)	876
MwoI	GCNNNNNNNGC	3 cut(s)	17, 38, 365
NarI	GGCGCC	1 cut(s)	64
NciI	CCSGG	1 cut(s)	260
NdeII	GATC	2 cut(s)	753, 933
NlaIII	CATG	8 cut(s)	88, 97, 176, 202, 476, 592, 805, 871
NlaIV	GGNNCC	6 cut(s)	65, 279, 444, 719, 948, 985
NmuCI	GTSAC	2 cut(s)	71, 503
OliI	CACNNNNGTG	1 cut(s)	78
PagI	TCATGA	1 cut(s)	801
PciSI	GCTCTTC	3 cut(s)	246, 661, 662
PctI	GAATGC	1 cut(s)	774
PdmI	GAANNNNTTC	1 cut(s)	1076
PfeI	GAWTC	1 cut(s)	286
PkrI	GCNGC	1 cut(s)	769
PleI	GAGTC	2 cut(s)	410, 1054
PluTI	GGCGCC	1 cut(s)	67
PpsI	GAGTC	2 cut(s)	410, 1054
Ppu21I	YACGTR	1 cut(s)	789
Psp6I	CCWGG	1 cut(s)	874
PspGI	CCWGG	1 cut(s)	874
PspN4I	GGNNCC	6 cut(s)	65, 279, 444, 719, 948, 985
PspPI	GGNCC	4 cut(s)	97, 262, 277, 1052
PstI	CTGCAG	1 cut(s)	568
PstNI	CAGNNNCTG	1 cut(s)	972
RsaI	GTAC	5 cut(s)	397, 791, 849, 1006, 1040
RsaNI	GTAC	5 cut(s)	396, 790, 848, 1005, 1039
RseI	CAYNNNNRTG	1 cut(s)	78
SapI	GCTCTTC	3 cut(s)	246, 661, 662
SaqAI	TTAA	2 cut(s)	149, 336
SatI	GCNGC	1 cut(s)	768
Sau3AI	GATC	2 cut(s)	753, 933
Sau96I	GGNCC	4 cut(s)	97, 262, 277, 1052
SchI	GAGTC	2 cut(s)	410, 1055
ScrFI	CCNGG	2 cut(s)	260, 876
SduI	GDGCHC	2 cut(s)	258, 414
SfaNI	GCATC	4 cut(s)	19, 97, 223, 713
SfcI	CTRYAG	1 cut(s)	564
SfoI	GGCGCC	1 cut(s)	65
SinI	GGWCC	1 cut(s)	262
SmiMI	CAYNNNNRTG	1 cut(s)	78
Sse9I	AATT	2 cut(s)	528, 576
SsiI	CCGC	1 cut(s)	715
SspDI	GGCGCC	1 cut(s)	63
SspMI	CTAG	2 cut(s)	51, 914
StyD4I	CCNGG	2 cut(s)	258, 874
TaaI	ACNGT	2 cut(s)	442, 968
TaiI	ACGT	1 cut(s)	791
TaqI	TCGA	1 cut(s)	414
TasI	AATT	2 cut(s)	528, 576
TatI	WGTACW	1 cut(s)	847
TfiI	GAWTC	1 cut(s)	286
Tru1I	TTAA	2 cut(s)	149, 336
Tru9I	TTAA	2 cut(s)	149, 336
TscAI	CASTG	5 cut(s)	82, 517, 568, 913, 973
TseFI	GTSAC	2 cut(s)	71, 503
TseI	GCWGC	1 cut(s)	767
Tsp45I	GTSAC	2 cut(s)	71, 503
TspDTI	ATGAA	4 cut(s)	539, 605, 818, 944
TspGWI	ACGGA	1 cut(s)	551
TspRI	CASTG	5 cut(s)	82, 517, 568, 913, 973
VpaK11BI	GGWCC	1 cut(s)	262
XapI	RAATTY	1 cut(s)	528
XmnI	GAANNNNTTC	1 cut(s)	1076
XspI	CTAG	2 cut(s)	51, 914

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.