Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0014908

Physical Location & Seq

Genomic Coordinates Forward (+)

29786630 .. 29788943

Gene Structure 2314 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1201800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGCGTGAGATTCTCCACATCCAGGGAGGCCAGTGCGGCAACCAGATCGGCGCCAAGTTCTGGGAGGTCGTCTGCGCCGAGCACGGCATCGACGCCACCGGCCGATACCAGGGCGACAACGAGCTCCAGCTCGAGCGCGTCAATGTCTACTACAACGAAGCCAGCTGTGGGAGGTTCGTCCCACGCGCCGTCCTCATGGATCTCGAGCCGGGAACCATGGACAGCGTCAGATCAGGGCCCTACGGCCAGATTTTCCGCCCTGATAACTTCGTCTTCGGCCAGTCCGGCGCCGGAAACAACTGGGCTAAGGGTCATTACACCGAAGGCGCCGAGTTGATCGACTCGGTCCTCGACGTCGTCAGGAAGGAAGCCGAGAACTGCGATTGCTTGCAAGGTTTTCAGGTTTGTCACTCTTTGGGAGGCGGGACTGGGTCCGGAATGGGAACTCTTCTCATTTCCAAGATCCGTGAGGAGTACCCGGACCGAATGATGCTCACATTCTCCGTGTTTCCGTCGCCGAAGGTGTCCGACACCGTCGTTGAGCCATACAATGCAACTCTCTCAGTTCACCAGCTTGTTGAAAATGCAGATGAGTGTATGGTTTTGGACAACGAAGCTCTCTACGACATTTGCTTCCGAACACTGAAGCTCACTACTCCCAGCTTTGGCGACCTCAATCATCTTATTTCTGCTACCATGAGTGGCGTAACCTGCTGCCTTCGTTTCCCTGGTCAACTCAACTCAGATCTCCGCAAGCTTGCTGTTAATCTCATCCCATTCCCCCGATTGCACTTCTTCATGGTTGGGTTTGCTCCGCTCACATCCCGTGGATCCCAGCAGTACAGGGCCCTTACTGTTCCAGAGCTCACTCAACAGATGTGGGATGCAAAGAACATGATGTGCGCCGCTGATCCACGTCATGGGCGGTATTTGACTGCCTCAGCCATGTTCCGTGGCAAGATGAGCACCAAGGAAGTTGACGAACAGATGATCAATGTCCAGAACAAGAACTCGTCCTACTTTGTTGAGTGGATCCCGAACAATGTCAAGTCCACTGTTTGTGATATTCCACCAACGGGTCTGAAGATGGCTTCGACCTTCATTGGCAACTCCACATCCATTCAAGAAATGTTCAGGAGGGTGAGTGAGCAGTTCACTGCTATGTTCCGCAGAAAGGCTTTCTTGCATTGGTACACCGGAGAGGGAATGGACGAGATGGAGTTCACCGAGGCAGAGAGCAACATGAACGACCTTGTTTCGGAGTACCAGCAGTACCAGGATGCTACAGCCGATGAGGAGGGTTACGACTATGAAGAGGAAGAGGAAGTTCAGGAGGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005200 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005856 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007017 GO:0008150 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045298 GO:0050896 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko04145 ko04540 ko05130 map04145 map04540 map05130

Pfam Domains

Protein Families

Tubulin Tubulin_C

Protein Analysis

448

Amino Acids

50.31

Weight (kDa)

4.76

Isoelectric Point (pI)

38.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tubulin	PF00091	3 - 211	2e-64	Tubulin/FtsZ family, GTPase domain
Tubulin_C	PF03953	261 - 382	1.7e-40	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014908)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23860 AT5G23860
fragaria_vesca	FvH4_7g29550
malus_domestica	MD01G1201800.v1.1 MD07G1270800.v1.1
prunus_persica	Prupe.2G295200_v2.0.a1
pyrus_communis	pycom01g21150
rosa_chinensis	RchiOBHm_Chr1g0377541
rosa_laevigata	RLG00000026521
rosa_multiflora	Rmu_sc0002832.1_g000005 Rmu_sc0012587.1_g000003
rosa_roxburghii	Rroxscaffold_4G00281250
rosa_rugosa	Rorug01G0403300
rosa_samantha	Rh1CG394000 Rh1DG411000
rosa_wichuraiana	Rw1G036930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	357
Acc36I	ACCTGC	1 cut(s)	719
AccB1I	GGYRCC	3 cut(s)	50, 287, 326
AccB7I	CCANNNNNTGG	2 cut(s)	60, 920
AccBSI	CCGCTC	1 cut(s)	817
AccI	GTMKAC	1 cut(s)	147
AccII	CGCG	2 cut(s)	138, 186
AccIII	TCCGGA	1 cut(s)	434
AciI	CCGC	8 cut(s)	36, 256, 423, 751, 815, 906, 925, 1168
AclWI	GGATC	7 cut(s)	207, 457, 825, 838, 905, 1027, 1040
AcoI	YGGCCR	3 cut(s)	100, 244, 277
AcuI	CTGAAG	2 cut(s)	665, 1103
AcyI	GRCGYC	5 cut(s)	51, 93, 288, 327, 354
AfaI	GTAC	5 cut(s)	476, 842, 1193, 1265, 1274
AfiI	CCNNNNNNNGG	9 cut(s)	22, 60, 109, 168, 665, 920, 1076, 1174, 1258
AgsI	TTSAA	2 cut(s)	581, 1124
AjiI	CACGTC	1 cut(s)	917
AjnI	CCWGG	4 cut(s)	21, 108, 727, 1275
AluBI	AGCT	9 cut(s)	124, 130, 165, 574, 617, 649, 663, 757, 865
AluI	AGCT	9 cut(s)	124, 130, 165, 574, 617, 649, 663, 757, 865
Alw21I	GWGCWC	4 cut(s)	84, 126, 867, 968
AlwI	GGATC	7 cut(s)	207, 457, 825, 838, 905, 1027, 1040
Ama87I	CYCGRG	2 cut(s)	131, 203
Aor13HI	TCCGGA	1 cut(s)	434
AoxI	GGCC	6 cut(s)	28, 100, 236, 244, 277, 846
ApaI	GGGCCC	2 cut(s)	240, 850
ApeKI	GCWGC	1 cut(s)	714
AspLEI	GCGC	7 cut(s)	53, 77, 138, 188, 290, 329, 905
AspS9I	GGNCC	7 cut(s)	236, 237, 346, 432, 481, 846, 847
AsuC2I	CCSGG	2 cut(s)	210, 479
AsuHPI	GGTGA	3 cut(s)	560, 1153, 1216
AvaI	CYCGRG	2 cut(s)	131, 203
AvaII	GGWCC	3 cut(s)	346, 432, 481
BaeGI	GKGCMC	2 cut(s)	240, 850
BamHI	GGATCC	2 cut(s)	830, 1032
BanI	GGYRCC	3 cut(s)	50, 287, 326
BanII	GRGCYC	4 cut(s)	126, 240, 850, 867
BbsI	GAAGAC	1 cut(s)	265
Bbv12I	GWGCWC	4 cut(s)	84, 126, 867, 968
BbvCI	CCTCAGC	1 cut(s)	940
BbvI	GCAGC	1 cut(s)	701
BccI	CCATC	2 cut(s)	1081, 1210
BceAI	ACGGC	3 cut(s)	100, 173, 259
BcgI	CGANNNNNNTGC	2 cut(s)	28, 62
BciT130I	CCWGG	4 cut(s)	23, 110, 729, 1277
BclI	TGATCA	1 cut(s)	990
BcnI	CCSGG	2 cut(s)	210, 479
BfmI	CTRYAG	1 cut(s)	1284
BfoI	RGCGCY	3 cut(s)	54, 291, 330
BfuAI	ACCTGC	1 cut(s)	719
BglI	GCCNNNNNGGC	2 cut(s)	36, 285
BglII	AGATCT	1 cut(s)	745
BisI	GCNGC	3 cut(s)	37, 715, 906
BlsI	GCNGC	3 cut(s)	38, 716, 907
Bme1390I	CCNGG	6 cut(s)	23, 110, 210, 479, 729, 1277
Bme18I	GGWCC	3 cut(s)	346, 432, 481
BmeT110I	CYCGRG	2 cut(s)	131, 203
BmgBI	CACGTC	1 cut(s)	917
BmgT120I	GGNCC	7 cut(s)	236, 237, 346, 432, 481, 846, 847
BmiI	GGNNCC	9 cut(s)	52, 214, 238, 289, 328, 433, 832, 848, 1034
BmrFI	CCNGG	6 cut(s)	23, 110, 210, 479, 729, 1277
BmrI	ACTGGG	2 cut(s)	310, 438
BmsI	GCATC	4 cut(s)	96, 480, 874, 1270
BmuI	ACTGGG	2 cut(s)	310, 438
BpiI	GAAGAC	1 cut(s)	265
BpmI	CTGGAG	1 cut(s)	110
Bpu10I	CCTNAGC	2 cut(s)	306, 940
BpuMI	CCSGG	2 cut(s)	210, 479
BsaHI	GRCGYC	5 cut(s)	51, 93, 288, 327, 354
BsaJI	CCNNGG	8 cut(s)	22, 109, 216, 727, 826, 952, 969, 1227
BsaWI	WCCGGW	2 cut(s)	434, 1196
BsaXI	ACNNNNNCTCC	2 cut(s)	1129, 1159
Bsc4I	CCNNNNNNNGG	9 cut(s)	22, 60, 109, 168, 665, 920, 1076, 1174, 1258
Bse118I	RCCGGY	1 cut(s)	98
Bse1I	ACTGG	4 cut(s)	31, 280, 305, 433
BseAI	TCCGGA	1 cut(s)	434
BseBI	CCWGG	4 cut(s)	23, 110, 729, 1277
BseDI	CCNNGG	8 cut(s)	22, 109, 216, 727, 826, 952, 969, 1227
BseGI	GGATG	6 cut(s)	18, 771, 821, 889, 1115, 1285
BseLI	CCNNNNNNNGG	9 cut(s)	22, 60, 109, 168, 665, 920, 1076, 1174, 1258
BseMII	CTCAG	3 cut(s)	576, 756, 954
BseNI	ACTGG	4 cut(s)	31, 280, 305, 433
BseRI	GAGGAG	2 cut(s)	485, 1310
BseSI	GKGCMC	2 cut(s)	240, 850
BseX3I	CGGCCG	1 cut(s)	100
BseXI	GCAGC	1 cut(s)	701
BseYI	CCCAGC	2 cut(s)	659, 834
Bsh1236I	CGCG	2 cut(s)	138, 186
Bsh1285I	CGRYCG	1 cut(s)	103
BshFI	GGCC	6 cut(s)	30, 102, 238, 246, 279, 848
BshNI	GGYRCC	3 cut(s)	50, 287, 326
BsiEI	CGRYCG	1 cut(s)	103
BsiHKAI	GWGCWC	4 cut(s)	84, 126, 867, 968
BsiHKCI	CYCGRG	2 cut(s)	131, 203
BsiSI	CCGG	7 cut(s)	99, 209, 285, 291, 435, 479, 1197
BslFI	GGGAC	2 cut(s)	164, 439
BslI	CCNNNNNNNGG	9 cut(s)	22, 60, 109, 168, 665, 920, 1076, 1174, 1258
BsmFI	GGGAC	2 cut(s)	164, 439
BsnI	GGCC	6 cut(s)	30, 102, 238, 246, 279, 848
BsoBI	CYCGRG	2 cut(s)	131, 203
Bsp120I	GGGCCC	2 cut(s)	236, 846
Bsp1286I	GDGCHC	6 cut(s)	84, 126, 240, 850, 867, 968
Bsp13I	TCCGGA	1 cut(s)	434
Bsp19I	CCATGG	1 cut(s)	216
BspACI	CCGC	8 cut(s)	36, 256, 423, 751, 815, 906, 925, 1168
BspANI	GGCC	6 cut(s)	30, 102, 238, 246, 279, 848
BspCNI	CTCAG	3 cut(s)	575, 755, 953
BspEI	TCCGGA	1 cut(s)	434
BspFNI	CGCG	2 cut(s)	138, 186
BspLI	GGNNCC	9 cut(s)	52, 214, 238, 289, 328, 433, 832, 848, 1034
BspMI	ACCTGC	1 cut(s)	719
BspPI	GGATC	7 cut(s)	207, 457, 825, 838, 905, 1027, 1040
BspT107I	GGYRCC	3 cut(s)	50, 287, 326
BsrBI	CCGCTC	1 cut(s)	817
BsrFI	RCCGGY	1 cut(s)	98
BsrI	ACTGG	4 cut(s)	31, 280, 305, 433
BssAI	RCCGGY	1 cut(s)	98
BssECI	CCNNGG	8 cut(s)	22, 109, 216, 727, 826, 952, 969, 1227
BssNI	GRCGYC	5 cut(s)	51, 93, 288, 327, 354
BssT1I	CCWWGG	2 cut(s)	216, 969
Bst2UI	CCWGG	4 cut(s)	23, 110, 729, 1277
Bst4CI	ACNGT	3 cut(s)	535, 856, 1057
Bst6I	CTCTTC	3 cut(s)	453, 1308, 1314
BstACI	GRCGYC	5 cut(s)	51, 93, 288, 327, 354
BstC8I	GCNNGC	4 cut(s)	163, 389, 755, 759
BstDEI	CTNAG	4 cut(s)	306, 562, 742, 940
BstDSI	CCRYGG	3 cut(s)	216, 826, 952
BstF5I	GGATG	6 cut(s)	18, 771, 821, 889, 1115, 1285
BstFNI	CGCG	2 cut(s)	138, 186
BstH2I	RGCGCY	3 cut(s)	54, 291, 330
BstHHI	GCGC	7 cut(s)	53, 77, 138, 188, 290, 329, 905
BstMCI	CGRYCG	1 cut(s)	103
BstMWI	GCNNNNNNNGC	4 cut(s)	36, 285, 711, 963
BstNI	CCWGG	4 cut(s)	23, 110, 729, 1277
BstSCI	CCNGG	6 cut(s)	21, 108, 208, 477, 727, 1275
BstSFI	CTRYAG	1 cut(s)	1284
BstSLI	GKGCMC	2 cut(s)	240, 850
BstUI	CGCG	2 cut(s)	138, 186
BstV1I	GCAGC	1 cut(s)	701
BstV2I	GAAGAC	1 cut(s)	265
BstX2I	RGATCY	5 cut(s)	199, 462, 745, 830, 1032
BstYI	RGATCY	5 cut(s)	199, 462, 745, 830, 1032
BstZI	CGGCCG	1 cut(s)	100
BsuRI	GGCC	6 cut(s)	30, 102, 238, 246, 279, 848
BtgI	CCRYGG	3 cut(s)	216, 826, 952
BtrI	CACGTC	1 cut(s)	917
BtsCI	GGATG	6 cut(s)	18, 771, 821, 889, 1115, 1285
BtsI	GCAGTG	1 cut(s)	1155
BtsIMutI	CAGTG	4 cut(s)	38, 641, 1053, 1155
BveI	ACCTGC	1 cut(s)	719
Cac8I	GCNNGC	4 cut(s)	163, 389, 755, 759
CfoI	GCGC	7 cut(s)	53, 77, 138, 188, 290, 329, 905
Cfr10I	RCCGGY	1 cut(s)	98
Cfr13I	GGNCC	7 cut(s)	236, 237, 346, 432, 481, 846, 847
CpoI	CGGWCCG	1 cut(s)	481
CseI	GACGC	3 cut(s)	101, 127, 214
Csp6I	GTAC	5 cut(s)	475, 841, 1192, 1264, 1273
CspCI	CAANNNNNGTGG	2 cut(s)	860, 895
CspI	CGGWCCG	1 cut(s)	481
CviAII	CATG	8 cut(s)	196, 217, 697, 799, 895, 920, 946, 1243
CviQI	GTAC	5 cut(s)	475, 841, 1192, 1264, 1273
DdeI	CTNAG	4 cut(s)	306, 562, 742, 940
DinI	GGCGCC	3 cut(s)	52, 289, 328
EaeI	YGGCCR	3 cut(s)	100, 244, 277
EagI	CGGCCG	1 cut(s)	100
Eam1104I	CTCTTC	3 cut(s)	453, 1308, 1314
EarI	CTCTTC	3 cut(s)	453, 1308, 1314
EciI	GGCGGA	1 cut(s)	245
Ecl136II	GAGCTC	2 cut(s)	124, 865
EclXI	CGGCCG	1 cut(s)	100
Eco130I	CCWWGG	2 cut(s)	216, 969
Eco24I	GRGCYC	4 cut(s)	126, 240, 850, 867
Eco47I	GGWCC	3 cut(s)	346, 432, 481
Eco52I	CGGCCG	1 cut(s)	100
Eco53kI	GAGCTC	2 cut(s)	124, 865
Eco57I	CTGAAG	2 cut(s)	665, 1103
Eco88I	CYCGRG	2 cut(s)	131, 203
EcoICRI	GAGCTC	2 cut(s)	124, 865
EcoO109I	RGGNCCY	4 cut(s)	236, 237, 846, 847
EcoRII	CCWGG	4 cut(s)	21, 108, 727, 1275
EcoT14I	CCWWGG	2 cut(s)	216, 969
EcoT38I	GRGCYC	4 cut(s)	126, 240, 850, 867
EgeI	GGCGCC	3 cut(s)	52, 289, 328
EheI	GGCGCC	3 cut(s)	52, 289, 328
ErhI	CCWWGG	2 cut(s)	216, 969
FaeI	CATG	8 cut(s)	199, 220, 700, 802, 898, 923, 949, 1246
FalI	AAGNNNNNCTT	2 cut(s)	1166, 1198
FaqI	GGGAC	2 cut(s)	164, 439
FatI	CATG	8 cut(s)	195, 216, 696, 798, 894, 919, 945, 1242
FauI	CCCGC	1 cut(s)	416
FbaI	TGATCA	1 cut(s)	990
FblI	GTMKAC	1 cut(s)	147
Fnu4HI	GCNGC	3 cut(s)	37, 715, 906
FokI	GGATG	6 cut(s)	5, 758, 808, 896, 1102, 1292
FriOI	GRGCYC	4 cut(s)	126, 240, 850, 867
Fsp4HI	GCNGC	3 cut(s)	37, 715, 906
GlaI	GCGC	7 cut(s)	52, 76, 137, 187, 289, 328, 904
GluI	GCNGC	3 cut(s)	37, 715, 906
GsaI	CCCAGC	2 cut(s)	663, 838
GsuI	CTGGAG	1 cut(s)	110
HaeII	RGCGCY	3 cut(s)	54, 291, 330
HaeIII	GGCC	6 cut(s)	30, 102, 238, 246, 279, 848
HapII	CCGG	7 cut(s)	99, 209, 285, 291, 435, 479, 1197
HgaI	GACGC	3 cut(s)	101, 127, 214
HhaI	GCGC	7 cut(s)	53, 77, 138, 188, 290, 329, 905
Hin1I	GRCGYC	5 cut(s)	51, 93, 288, 327, 354
Hin1II	CATG	8 cut(s)	199, 220, 700, 802, 898, 923, 949, 1246
Hin6I	GCGC	7 cut(s)	51, 75, 136, 186, 288, 327, 903
HinP1I	GCGC	7 cut(s)	51, 75, 136, 186, 288, 327, 903
HincII	GTYRAC	2 cut(s)	734, 979
HindII	GTYRAC	2 cut(s)	734, 979
HindIII	AAGCTT	1 cut(s)	755
HinfI	GANTC	2 cut(s)	10, 341
HpaII	CCGG	7 cut(s)	99, 209, 285, 291, 435, 479, 1197
HphI	GGTGA	3 cut(s)	560, 1153, 1216
Hpy166II	GTNNAC	8 cut(s)	148, 568, 734, 979, 1053, 1155, 1194, 1224
Hpy188I	TCNGA	6 cut(s)	230, 529, 638, 745, 1083, 1261
Hpy188III	TCNNGA	9 cut(s)	203, 361, 435, 860, 1000, 1036, 1124, 1135, 1331
Hpy8I	GTNNAC	8 cut(s)	148, 568, 734, 979, 1053, 1155, 1194, 1224
Hpy99I	CGWCG	5 cut(s)	95, 356, 359, 517, 539
HpyAV	CCTTC	5 cut(s)	317, 358, 514, 728, 1108
HpyCH4III	ACNGT	3 cut(s)	535, 856, 1057
HpyCH4IV	ACGT	2 cut(s)	354, 916
HpyCH4V	TGCA	6 cut(s)	391, 554, 587, 790, 887, 1186
HpyF10VI	GCNNNNNNNGC	4 cut(s)	36, 285, 711, 963
HpyF3I	CTNAG	4 cut(s)	306, 562, 742, 940
HpySE526I	ACGT	2 cut(s)	354, 916
Hsp92I	GRCGYC	5 cut(s)	51, 93, 288, 327, 354
Hsp92II	CATG	8 cut(s)	199, 220, 700, 802, 898, 923, 949, 1246
HspAI	GCGC	7 cut(s)	51, 75, 136, 186, 288, 327, 903
KasI	GGCGCC	3 cut(s)	50, 287, 326
Kpn2I	TCCGGA	1 cut(s)	434
Ksp22I	TGATCA	1 cut(s)	990
LmnI	GCTCC	2 cut(s)	129, 817
Lsp1109I	GCAGC	1 cut(s)	701
LweI	GCATC	4 cut(s)	96, 480, 874, 1270
MaeII	ACGT	2 cut(s)	354, 916
MaeIII	GTNAC	3 cut(s)	407, 706, 1301
MbiI	CCGCTC	1 cut(s)	817
MboII	GAAGA	6 cut(s)	265, 440, 787, 1096, 1325, 1331
MflI	RGATCY	5 cut(s)	199, 462, 745, 830, 1032
MhlI	GDGCHC	6 cut(s)	84, 126, 240, 850, 867, 968
Mly113I	GGCGCC	3 cut(s)	51, 288, 327
MlyI	GAGTC	1 cut(s)	335
MmeI	TCCRAC	1 cut(s)	552
MroI	TCCGGA	1 cut(s)	434
MseI	TTAA	1 cut(s)	765
MslI	CAYNNNNRTG	1 cut(s)	1160
MspA1I	CMGCKG	2 cut(s)	165, 908
MspI	CCGG	7 cut(s)	99, 209, 285, 291, 435, 479, 1197
MspR9I	CCNGG	6 cut(s)	23, 110, 210, 479, 729, 1277
MvaI	CCWGG	4 cut(s)	23, 110, 729, 1277
MvnI	CGCG	2 cut(s)	138, 186
MwoI	GCNNNNNNNGC	4 cut(s)	36, 285, 711, 963
NarI	GGCGCC	3 cut(s)	51, 288, 327
NciI	CCSGG	2 cut(s)	210, 479
NcoI	CCATGG	1 cut(s)	216
NlaIII	CATG	8 cut(s)	199, 220, 700, 802, 898, 923, 949, 1246
NlaIV	GGNNCC	9 cut(s)	52, 214, 238, 289, 328, 433, 832, 848, 1034
NmeAIII	GCCGAG	3 cut(s)	103, 355, 397
NmuCI	GTSAC	1 cut(s)	407
PaeR7I	CTCGAG	2 cut(s)	131, 203
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PfeI	GAWTC	1 cut(s)	10
PflFI	GACNNNGTC	5 cut(s)	224, 344, 356, 430, 533
PflMI	CCANNNNNTGG	2 cut(s)	60, 920
PkrI	GCNGC	3 cut(s)	38, 716, 907
PleI	GAGTC	1 cut(s)	335
PluTI	GGCGCC	3 cut(s)	54, 291, 330
PpsI	GAGTC	1 cut(s)	335
Psp124BI	GAGCTC	2 cut(s)	126, 867
Psp6I	CCWGG	4 cut(s)	21, 108, 727, 1275
PspFI	CCCAGC	2 cut(s)	659, 834
PspGI	CCWGG	4 cut(s)	21, 108, 727, 1275
PspN4I	GGNNCC	9 cut(s)	52, 214, 238, 289, 328, 433, 832, 848, 1034
PspOMI	GGGCCC	2 cut(s)	236, 846
PspPI	GGNCC	7 cut(s)	236, 237, 346, 432, 481, 846, 847
PspXI	VCTCGAGB	1 cut(s)	131
PsrI	GAACNNNNNNTAC	2 cut(s)	1001, 1033
PsuI	RGATCY	5 cut(s)	199, 462, 745, 830, 1032
PsyI	GACNNNGTC	5 cut(s)	224, 344, 356, 430, 533
PvuII	CAGCTG	1 cut(s)	165
RsaI	GTAC	5 cut(s)	476, 842, 1193, 1265, 1274
RsaNI	GTAC	5 cut(s)	475, 841, 1192, 1264, 1273
RseI	CAYNNNNRTG	1 cut(s)	1160
Rsr2I	CGGWCCG	1 cut(s)	481
RsrII	CGGWCCG	1 cut(s)	481
SacI	GAGCTC	2 cut(s)	126, 867
SaqAI	TTAA	1 cut(s)	765
SatI	GCNGC	3 cut(s)	37, 715, 906
Sau96I	GGNCC	7 cut(s)	236, 237, 346, 432, 481, 846, 847
SchI	GAGTC	1 cut(s)	335
ScrFI	CCNGG	6 cut(s)	23, 110, 210, 479, 729, 1277
SduI	GDGCHC	6 cut(s)	84, 126, 240, 850, 867, 968
SfaNI	GCATC	4 cut(s)	96, 480, 874, 1270
SfcI	CTRYAG	1 cut(s)	1284
SfoI	GGCGCC	3 cut(s)	52, 289, 328
Sfr274I	CTCGAG	2 cut(s)	131, 203
SinI	GGWCC	3 cut(s)	346, 432, 481
SlaI	CTCGAG	2 cut(s)	131, 203
SmiMI	CAYNNNNRTG	1 cut(s)	1160
SmlI	CTYRAG	2 cut(s)	131, 203
SmoI	CTYRAG	2 cut(s)	131, 203
SsiI	CCGC	8 cut(s)	36, 256, 423, 751, 815, 906, 925, 1168
SspDI	GGCGCC	3 cut(s)	50, 287, 326
SstI	GAGCTC	2 cut(s)	126, 867
StyD4I	CCNGG	6 cut(s)	21, 108, 208, 477, 727, 1275
StyI	CCWWGG	2 cut(s)	216, 969
TaaI	ACNGT	3 cut(s)	535, 856, 1057
TaiI	ACGT	2 cut(s)	357, 919
TaqI	TCGA	6 cut(s)	90, 132, 204, 339, 351, 1094
TaqII	GACCGA	2 cut(s)	334, 498
TatI	WGTACW	1 cut(s)	840
TauI	GCSGC	2 cut(s)	39, 908
TfiI	GAWTC	1 cut(s)	10
Tru1I	TTAA	1 cut(s)	765
Tru9I	TTAA	1 cut(s)	765
TscAI	CASTG	4 cut(s)	38, 648, 1060, 1162
TseFI	GTSAC	1 cut(s)	407
TseI	GCWGC	1 cut(s)	714
Tsp45I	GTSAC	1 cut(s)	407
TspDTI	ATGAA	4 cut(s)	787, 1090, 1259, 1326
TspGWI	ACGGA	4 cut(s)	455, 493, 501, 941
TspRI	CASTG	4 cut(s)	38, 648, 1060, 1162
Tth111I	GACNNNGTC	5 cut(s)	224, 344, 356, 430, 533
Van91I	CCANNNNNTGG	2 cut(s)	60, 920
VpaK11BI	GGWCC	3 cut(s)	346, 432, 481
XhoI	CTCGAG	2 cut(s)	131, 203
XmiI	GTMKAC	1 cut(s)	147
ZraI	GACGTC	1 cut(s)	355

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.