Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0014908

Physical Location & Seq

Genomic Coordinates Forward (+)

33778027 .. 33780764

Gene Structure 2738 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1270800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGCGTGAGATTCTCCACATCCAGGGAGGCCAGTGCGGGAACCAGATCGGCGCCAAGTTCTGGGAGGTCGTCTGCGCCGAGCACGGCATCGACTCCACCGGCCGATACCAGGGCGACAACGAGCTCCAGCTCGAGCGCGTCAATGTCTACTACAACGAGGCCAGTTGCGGGAGGTTTGTCCCACGCGCCGTCCTCATGGATCTCGAGCCGGGAACCATGGACAGCGTCAGATCTGGGCCATACGGCCAGATTTTCCGCCCAGATAACTTCGTTTTCGGCCAGTCCGGCGCCGGAAACAACTGGGCCAAGGGCCACTACACCGAAGGCGCCGAGTTGATCGACTCCGTTCTCGACGTCGTCAGGAAGGAGGCCGAGAACTGCGATTGCTTGCAAGGATTTCAGGTTTGTCACTCTTTGGGAGGCGGGACTGGGTCCGGAATGGGAACTCTTCTCATTTCCAAGATCCGAGAGGAGTATCCGGACCGAATGATGCTCACATTCTCCGTGTTTCCGTCGCCAAAGGTGTCTGACACCGTCGTTGAGCCATACAATGCAACTCTCTCTGTTCACCAGCTTGTTGAAAATGCAGATGAGTGTATGGTTTTGGACAACGAAGCTCTCTACGACATTTGCTTCCGGACACTGAAGCTCACTACTCCCAGCTTTGGCGACCTCAATCATCTTATTTCTGCTACCATGAGTGGCGTAACTTGCTGCCTTCGTTTCCCTGGTCAACTCAACTCAGATCTTCGCAAGCTTGCTGTTAATCTCATCCCATTCCCCCGACTGCACTTCTTCATGGTTGGGTTTGCTCCACTCACATCCCGCGGATCACAGCAGTACAGGGCCCTTACTGTTCCAGAGCTCACTCAACAGATGTGGGATGCAAAGAACATGATGTGCGCCGCTGATCCACGTCATGGACGGTATTTGACTGCTTCAGCCATGTTCCGTGGCAAGATGAGCACCAAGGAAGTTGACGAACAGATGATCAATGTGCAAAACAAGAACTCATCCTACTTTGTTGAGTGGATCCCAAACAATGTCAAGTCCACTGTTTGTGATATTCCACCAACCGGTCTGAAGATGGCCTCGACCTTCATTGGTAACTCCACCTCCATTCAGGAAATGTTCAGGAGGGTGAGTGAGCAGTTCACTGCTATGTTCCGAAGAAAGGCTTTCTTGCATTGGTACACCGGAGAGGGAATGGATGAGATGGAGTTCACTGAGGCAGAGAGCAACATGAATGACCTTGTTTCAGAGTACCAGCAGTACCAGGATGCTACAGCCGATGAGGAGGGGTACGACTATGAAGAAGAGGAGGAAGCTCAGGAGGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005200 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005856 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007017 GO:0008150 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045298 GO:0050896 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko04145 ko04540 ko05130 map04145 map04540 map05130

Pfam Domains

Protein Families

Tubulin Tubulin_C

Protein Analysis

448

Amino Acids

50.29

Weight (kDa)

4.76

Isoelectric Point (pI)

39.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tubulin	PF00091	3 - 211	2e-64	Tubulin/FtsZ family, GTPase domain
Tubulin_C	PF03953	261 - 382	1.7e-40	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014908)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23860 AT5G23860
fragaria_vesca	FvH4_7g29550
malus_domestica	MD01G1201800.v1.1 MD07G1270800.v1.1
prunus_persica	Prupe.2G295200_v2.0.a1
pyrus_communis	pycom01g21150
rosa_chinensis	RchiOBHm_Chr1g0377541
rosa_laevigata	RLG00000026521
rosa_multiflora	Rmu_sc0002832.1_g000005 Rmu_sc0012587.1_g000003
rosa_roxburghii	Rroxscaffold_4G00281250
rosa_rugosa	Rorug01G0403300
rosa_samantha	Rh1CG394000 Rh1DG411000
rosa_wichuraiana	Rw1G036930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	357
AccB1I	GGYRCC	3 cut(s)	50, 287, 326
AccB7I	CCANNNNNTGG	2 cut(s)	60, 920
AccI	GTMKAC	1 cut(s)	147
AccII	CGCG	3 cut(s)	138, 186, 828
AccIII	TCCGGA	3 cut(s)	434, 478, 636
AciI	CCGC	7 cut(s)	36, 168, 256, 423, 826, 828, 906
AclWI	GGATC	6 cut(s)	207, 457, 838, 905, 1027, 1040
AcoI	YGGCCR	3 cut(s)	100, 244, 277
AcuI	CTGAAG	3 cut(s)	665, 924, 1103
AcyI	GRCGYC	4 cut(s)	51, 288, 327, 354
AfaI	GTAC	5 cut(s)	842, 1193, 1265, 1274, 1304
AfiI	CCNNNNNNNGG	8 cut(s)	22, 60, 109, 168, 665, 920, 1076, 1174
AgeI	ACCGGT	1 cut(s)	1076
AgsI	TTSAA	1 cut(s)	581
AjiI	CACGTC	1 cut(s)	917
AjnI	CCWGG	4 cut(s)	21, 108, 727, 1275
AluBI	AGCT	9 cut(s)	124, 130, 574, 617, 649, 663, 757, 865, 1328
AluI	AGCT	9 cut(s)	124, 130, 574, 617, 649, 663, 757, 865, 1328
Alw21I	GWGCWC	4 cut(s)	84, 126, 867, 968
AlwI	GGATC	6 cut(s)	207, 457, 838, 905, 1027, 1040
Ama87I	CYCGRG	2 cut(s)	131, 203
Aor13HI	TCCGGA	3 cut(s)	434, 478, 636
ApaI	GGGCCC	1 cut(s)	850
ApeKI	GCWGC	1 cut(s)	714
AsiGI	ACCGGT	1 cut(s)	1076
AspLEI	GCGC	7 cut(s)	53, 77, 138, 188, 290, 329, 905
AspS9I	GGNCC	7 cut(s)	236, 303, 310, 432, 481, 846, 847
AsuC2I	CCSGG	1 cut(s)	210
AsuHPI	GGTGA	2 cut(s)	560, 1153
AvaI	CYCGRG	2 cut(s)	131, 203
AvaII	GGWCC	2 cut(s)	432, 481
BaeGI	GKGCMC	1 cut(s)	850
BamHI	GGATCC	1 cut(s)	1032
BanI	GGYRCC	3 cut(s)	50, 287, 326
BanII	GRGCYC	3 cut(s)	126, 850, 867
Bbv12I	GWGCWC	4 cut(s)	84, 126, 867, 968
BbvI	GCAGC	1 cut(s)	701
BccI	CCATC	2 cut(s)	1081, 1210
BceAI	ACGGC	3 cut(s)	100, 173, 259
BciT130I	CCWGG	4 cut(s)	23, 110, 729, 1277
BciVI	GTATCC	1 cut(s)	486
BclI	TGATCA	1 cut(s)	990
BcnI	CCSGG	1 cut(s)	210
BfmI	CTRYAG	1 cut(s)	1284
BfoI	RGCGCY	3 cut(s)	54, 291, 330
BfuI	GTATCC	1 cut(s)	486
BglI	GCCNNNNNGGC	1 cut(s)	285
BglII	AGATCT	2 cut(s)	230, 745
BisI	GCNGC	2 cut(s)	715, 906
BlsI	GCNGC	2 cut(s)	716, 907
Bme1390I	CCNGG	5 cut(s)	23, 110, 210, 729, 1277
Bme18I	GGWCC	2 cut(s)	432, 481
BmeT110I	CYCGRG	2 cut(s)	131, 203
BmgBI	CACGTC	1 cut(s)	917
BmgT120I	GGNCC	7 cut(s)	236, 303, 310, 432, 481, 846, 847
BmiI	GGNNCC	8 cut(s)	41, 52, 214, 289, 328, 433, 848, 1034
BmrFI	CCNGG	5 cut(s)	23, 110, 210, 729, 1277
BmrI	ACTGGG	2 cut(s)	310, 438
BmsI	GCATC	4 cut(s)	96, 480, 874, 1270
BmuI	ACTGGG	2 cut(s)	310, 438
BpmI	CTGGAG	1 cut(s)	110
Bpu10I	CCTNAGC	1 cut(s)	1329
BpuMI	CCSGG	1 cut(s)	210
BsaHI	GRCGYC	4 cut(s)	51, 288, 327, 354
BsaJI	CCNNGG	8 cut(s)	22, 109, 216, 306, 727, 826, 952, 969
BsaWI	WCCGGW	5 cut(s)	434, 478, 636, 1076, 1196
BsaXI	ACNNNNNCTCC	4 cut(s)	1100, 1129, 1130, 1159
Bsc4I	CCNNNNNNNGG	8 cut(s)	22, 60, 109, 168, 665, 920, 1076, 1174
Bse118I	RCCGGY	2 cut(s)	98, 1076
Bse1I	ACTGG	5 cut(s)	31, 162, 280, 305, 433
BseAI	TCCGGA	3 cut(s)	434, 478, 636
BseBI	CCWGG	4 cut(s)	23, 110, 729, 1277
BseDI	CCNNGG	8 cut(s)	22, 109, 216, 306, 727, 826, 952, 969
BseGI	GGATG	7 cut(s)	18, 771, 821, 889, 1013, 1216, 1285
BseLI	CCNNNNNNNGG	8 cut(s)	22, 60, 109, 168, 665, 920, 1076, 1174
BseMII	CTCAG	3 cut(s)	756, 1218, 1343
BseNI	ACTGG	5 cut(s)	31, 162, 280, 305, 433
BseRI	GAGGAG	3 cut(s)	485, 1310, 1334
BseSI	GKGCMC	1 cut(s)	850
BseX3I	CGGCCG	1 cut(s)	100
BseXI	GCAGC	1 cut(s)	701
BseYI	CCCAGC	1 cut(s)	659
BsgI	GTGCAG	1 cut(s)	773
Bsh1236I	CGCG	3 cut(s)	138, 186, 828
Bsh1285I	CGRYCG	1 cut(s)	103
BshNI	GGYRCC	3 cut(s)	50, 287, 326
BshTI	ACCGGT	1 cut(s)	1076
BsiEI	CGRYCG	1 cut(s)	103
BsiHKAI	GWGCWC	4 cut(s)	84, 126, 867, 968
BsiHKCI	CYCGRG	2 cut(s)	131, 203
BsiSI	CCGG	9 cut(s)	99, 209, 285, 291, 435, 479, 637, 1077, 1197
BslFI	GGGAC	2 cut(s)	164, 439
BslI	CCNNNNNNNGG	8 cut(s)	22, 60, 109, 168, 665, 920, 1076, 1174
BsmFI	GGGAC	2 cut(s)	164, 439
BsoBI	CYCGRG	2 cut(s)	131, 203
Bsp120I	GGGCCC	1 cut(s)	846
Bsp1286I	GDGCHC	5 cut(s)	84, 126, 850, 867, 968
Bsp13I	TCCGGA	3 cut(s)	434, 478, 636
Bsp19I	CCATGG	1 cut(s)	216
BspACI	CCGC	7 cut(s)	36, 168, 256, 423, 826, 828, 906
BspCNI	CTCAG	3 cut(s)	755, 1219, 1342
BspEI	TCCGGA	3 cut(s)	434, 478, 636
BspFNI	CGCG	3 cut(s)	138, 186, 828
BspLI	GGNNCC	8 cut(s)	41, 52, 214, 289, 328, 433, 848, 1034
BspPI	GGATC	6 cut(s)	207, 457, 838, 905, 1027, 1040
BspT107I	GGYRCC	3 cut(s)	50, 287, 326
BsrFI	RCCGGY	2 cut(s)	98, 1076
BsrI	ACTGG	5 cut(s)	31, 162, 280, 305, 433
BssAI	RCCGGY	2 cut(s)	98, 1076
BssECI	CCNNGG	8 cut(s)	22, 109, 216, 306, 727, 826, 952, 969
BssNI	GRCGYC	4 cut(s)	51, 288, 327, 354
BssT1I	CCWWGG	3 cut(s)	216, 306, 969
Bst2UI	CCWGG	4 cut(s)	23, 110, 729, 1277
Bst4CI	ACNGT	4 cut(s)	535, 856, 927, 1057
Bst6I	CTCTTC	2 cut(s)	453, 1311
BstACI	GRCGYC	4 cut(s)	51, 288, 327, 354
BstC8I	GCNNGC	3 cut(s)	389, 755, 759
BstDEI	CTNAG	3 cut(s)	742, 1227, 1329
BstDSI	CCRYGG	3 cut(s)	216, 826, 952
BstF5I	GGATG	7 cut(s)	18, 771, 821, 889, 1013, 1216, 1285
BstFNI	CGCG	3 cut(s)	138, 186, 828
BstH2I	RGCGCY	3 cut(s)	54, 291, 330
BstHHI	GCGC	7 cut(s)	53, 77, 138, 188, 290, 329, 905
BstMCI	CGRYCG	1 cut(s)	103
BstMWI	GCNNNNNNNGC	3 cut(s)	285, 711, 963
BstNI	CCWGG	4 cut(s)	23, 110, 729, 1277
BstSCI	CCNGG	5 cut(s)	21, 108, 208, 727, 1275
BstSFI	CTRYAG	1 cut(s)	1284
BstSLI	GKGCMC	1 cut(s)	850
BstUI	CGCG	3 cut(s)	138, 186, 828
BstV1I	GCAGC	1 cut(s)	701
BstX2I	RGATCY	5 cut(s)	199, 230, 462, 745, 1032
BstYI	RGATCY	5 cut(s)	199, 230, 462, 745, 1032
BstZI	CGGCCG	1 cut(s)	100
BsuI	GTATCC	1 cut(s)	486
BtgI	CCRYGG	3 cut(s)	216, 826, 952
BtrI	CACGTC	1 cut(s)	917
BtsCI	GGATG	7 cut(s)	18, 771, 821, 889, 1013, 1216, 1285
BtsI	GCAGTG	1 cut(s)	1155
BtsIMutI	CAGTG	5 cut(s)	38, 641, 1053, 1155, 1224
Cac8I	GCNNGC	3 cut(s)	389, 755, 759
CfoI	GCGC	7 cut(s)	53, 77, 138, 188, 290, 329, 905
Cfr10I	RCCGGY	2 cut(s)	98, 1076
Cfr13I	GGNCC	7 cut(s)	236, 303, 310, 432, 481, 846, 847
Cfr42I	CCGCGG	1 cut(s)	829
CpoI	CGGWCCG	1 cut(s)	481
CseI	GACGC	2 cut(s)	127, 214
Csp6I	GTAC	5 cut(s)	841, 1192, 1264, 1273, 1303
CspAI	ACCGGT	1 cut(s)	1076
CspCI	CAANNNNNGTGG	2 cut(s)	860, 895
CspI	CGGWCCG	1 cut(s)	481
CviAII	CATG	8 cut(s)	196, 217, 697, 799, 895, 920, 946, 1243
CviQI	GTAC	5 cut(s)	841, 1192, 1264, 1273, 1303
DdeI	CTNAG	3 cut(s)	742, 1227, 1329
DinI	GGCGCC	3 cut(s)	52, 289, 328
EaeI	YGGCCR	3 cut(s)	100, 244, 277
EagI	CGGCCG	1 cut(s)	100
Eam1104I	CTCTTC	2 cut(s)	453, 1311
EarI	CTCTTC	2 cut(s)	453, 1311
EciI	GGCGGA	1 cut(s)	245
Ecl136II	GAGCTC	2 cut(s)	124, 865
EclXI	CGGCCG	1 cut(s)	100
Eco130I	CCWWGG	3 cut(s)	216, 306, 969
Eco24I	GRGCYC	3 cut(s)	126, 850, 867
Eco47I	GGWCC	2 cut(s)	432, 481
Eco52I	CGGCCG	1 cut(s)	100
Eco53kI	GAGCTC	2 cut(s)	124, 865
Eco57I	CTGAAG	3 cut(s)	665, 924, 1103
Eco88I	CYCGRG	2 cut(s)	131, 203
EcoICRI	GAGCTC	2 cut(s)	124, 865
EcoO109I	RGGNCCY	2 cut(s)	846, 847
EcoRII	CCWGG	4 cut(s)	21, 108, 727, 1275
EcoT14I	CCWWGG	3 cut(s)	216, 306, 969
EcoT38I	GRGCYC	3 cut(s)	126, 850, 867
EgeI	GGCGCC	3 cut(s)	52, 289, 328
EheI	GGCGCC	3 cut(s)	52, 289, 328
ErhI	CCWWGG	3 cut(s)	216, 306, 969
FaeI	CATG	8 cut(s)	199, 220, 700, 802, 898, 923, 949, 1246
FalI	AAGNNNNNCTT	4 cut(s)	1162, 1194, 1166, 1198
FaqI	GGGAC	2 cut(s)	164, 439
FatI	CATG	8 cut(s)	195, 216, 696, 798, 894, 919, 945, 1242
FauI	CCCGC	4 cut(s)	29, 161, 416, 833
FbaI	TGATCA	1 cut(s)	990
FblI	GTMKAC	1 cut(s)	147
Fnu4HI	GCNGC	2 cut(s)	715, 906
FokI	GGATG	7 cut(s)	5, 758, 808, 896, 1000, 1223, 1292
FriOI	GRGCYC	3 cut(s)	126, 850, 867
Fsp4HI	GCNGC	2 cut(s)	715, 906
GlaI	GCGC	7 cut(s)	52, 76, 137, 187, 289, 328, 904
GluI	GCNGC	2 cut(s)	715, 906
GsaI	CCCAGC	1 cut(s)	663
GsuI	CTGGAG	1 cut(s)	110
HaeII	RGCGCY	3 cut(s)	54, 291, 330
HapII	CCGG	9 cut(s)	99, 209, 285, 291, 435, 479, 637, 1077, 1197
HgaI	GACGC	2 cut(s)	127, 214
HhaI	GCGC	7 cut(s)	53, 77, 138, 188, 290, 329, 905
Hin1I	GRCGYC	4 cut(s)	51, 288, 327, 354
Hin1II	CATG	8 cut(s)	199, 220, 700, 802, 898, 923, 949, 1246
Hin6I	GCGC	7 cut(s)	51, 75, 136, 186, 288, 327, 903
HinP1I	GCGC	7 cut(s)	51, 75, 136, 186, 288, 327, 903
HincII	GTYRAC	2 cut(s)	734, 979
HindII	GTYRAC	2 cut(s)	734, 979
HindIII	AAGCTT	1 cut(s)	755
HinfI	GANTC	3 cut(s)	10, 92, 341
HpaII	CCGG	9 cut(s)	99, 209, 285, 291, 435, 479, 637, 1077, 1197
HphI	GGTGA	2 cut(s)	560, 1153
Hpy166II	GTNNAC	8 cut(s)	148, 568, 734, 979, 1053, 1155, 1194, 1224
Hpy188I	TCNGA	7 cut(s)	230, 467, 529, 745, 1083, 1169, 1261
Hpy8I	GTNNAC	8 cut(s)	148, 568, 734, 979, 1053, 1155, 1194, 1224
Hpy99I	CGWCG	4 cut(s)	356, 359, 517, 539
HpyAV	CCTTC	4 cut(s)	317, 358, 728, 1108
HpyCH4III	ACNGT	4 cut(s)	535, 856, 927, 1057
HpyCH4IV	ACGT	2 cut(s)	354, 916
HpyCH4V	TGCA	7 cut(s)	391, 554, 587, 790, 887, 1000, 1186
HpyF10VI	GCNNNNNNNGC	3 cut(s)	285, 711, 963
HpyF3I	CTNAG	3 cut(s)	742, 1227, 1329
HpySE526I	ACGT	2 cut(s)	354, 916
Hsp92I	GRCGYC	4 cut(s)	51, 288, 327, 354
Hsp92II	CATG	8 cut(s)	199, 220, 700, 802, 898, 923, 949, 1246
HspAI	GCGC	7 cut(s)	51, 75, 136, 186, 288, 327, 903
KasI	GGCGCC	3 cut(s)	50, 287, 326
Kpn2I	TCCGGA	3 cut(s)	434, 478, 636
Ksp22I	TGATCA	1 cut(s)	990
KspI	CCGCGG	1 cut(s)	829
LmnI	GCTCC	2 cut(s)	129, 817
Lsp1109I	GCAGC	1 cut(s)	701
LweI	GCATC	4 cut(s)	96, 480, 874, 1270
MaeII	ACGT	2 cut(s)	354, 916
MaeIII	GTNAC	3 cut(s)	407, 706, 1106
MboII	GAAGA	7 cut(s)	440, 740, 787, 1096, 1182, 1325, 1328
MflI	RGATCY	5 cut(s)	199, 230, 462, 745, 1032
MhlI	GDGCHC	5 cut(s)	84, 126, 850, 867, 968
Mly113I	GGCGCC	3 cut(s)	51, 288, 327
MlyI	GAGTC	2 cut(s)	86, 335
MroI	TCCGGA	3 cut(s)	434, 478, 636
MseI	TTAA	1 cut(s)	765
MslI	CAYNNNNRTG	1 cut(s)	1160
MspA1I	CMGCKG	2 cut(s)	828, 908
MspI	CCGG	9 cut(s)	99, 209, 285, 291, 435, 479, 637, 1077, 1197
MspR9I	CCNGG	5 cut(s)	23, 110, 210, 729, 1277
MvaI	CCWGG	4 cut(s)	23, 110, 729, 1277
MvnI	CGCG	3 cut(s)	138, 186, 828
MwoI	GCNNNNNNNGC	3 cut(s)	285, 711, 963
NarI	GGCGCC	3 cut(s)	51, 288, 327
NciI	CCSGG	1 cut(s)	210
NcoI	CCATGG	1 cut(s)	216
NlaIII	CATG	8 cut(s)	199, 220, 700, 802, 898, 923, 949, 1246
NlaIV	GGNNCC	8 cut(s)	41, 52, 214, 289, 328, 433, 848, 1034
NmeAIII	GCCGAG	3 cut(s)	103, 355, 397
NmuCI	GTSAC	1 cut(s)	407
PaeR7I	CTCGAG	2 cut(s)	131, 203
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PfeI	GAWTC	1 cut(s)	10
PflFI	GACNNNGTC	4 cut(s)	224, 356, 430, 533
PflMI	CCANNNNNTGG	2 cut(s)	60, 920
PinAI	ACCGGT	1 cut(s)	1076
PkrI	GCNGC	2 cut(s)	716, 907
PleI	GAGTC	2 cut(s)	86, 335
PluTI	GGCGCC	3 cut(s)	54, 291, 330
PpsI	GAGTC	2 cut(s)	86, 335
Psp124BI	GAGCTC	2 cut(s)	126, 867
Psp6I	CCWGG	4 cut(s)	21, 108, 727, 1275
PspFI	CCCAGC	1 cut(s)	659
PspGI	CCWGG	4 cut(s)	21, 108, 727, 1275
PspN4I	GGNNCC	8 cut(s)	41, 52, 214, 289, 328, 433, 848, 1034
PspOMI	GGGCCC	1 cut(s)	846
PspPI	GGNCC	7 cut(s)	236, 303, 310, 432, 481, 846, 847
PspXI	VCTCGAGB	1 cut(s)	131
PsrI	GAACNNNNNNTAC	2 cut(s)	1001, 1033
PsuI	RGATCY	5 cut(s)	199, 230, 462, 745, 1032
PsyI	GACNNNGTC	4 cut(s)	224, 356, 430, 533
RsaI	GTAC	5 cut(s)	842, 1193, 1265, 1274, 1304
RsaNI	GTAC	5 cut(s)	841, 1192, 1264, 1273, 1303
RseI	CAYNNNNRTG	1 cut(s)	1160
Rsr2I	CGGWCCG	1 cut(s)	481
RsrII	CGGWCCG	1 cut(s)	481
SacI	GAGCTC	2 cut(s)	126, 867
SacII	CCGCGG	1 cut(s)	829
SaqAI	TTAA	1 cut(s)	765
SatI	GCNGC	2 cut(s)	715, 906
Sau96I	GGNCC	7 cut(s)	236, 303, 310, 432, 481, 846, 847
SchI	GAGTC	2 cut(s)	86, 335
ScrFI	CCNGG	5 cut(s)	23, 110, 210, 729, 1277
SduI	GDGCHC	5 cut(s)	84, 126, 850, 867, 968
SfaNI	GCATC	4 cut(s)	96, 480, 874, 1270
SfcI	CTRYAG	1 cut(s)	1284
SfoI	GGCGCC	3 cut(s)	52, 289, 328
Sfr274I	CTCGAG	2 cut(s)	131, 203
Sfr303I	CCGCGG	1 cut(s)	829
SgrBI	CCGCGG	1 cut(s)	829
SinI	GGWCC	2 cut(s)	432, 481
SlaI	CTCGAG	2 cut(s)	131, 203
SmiMI	CAYNNNNRTG	1 cut(s)	1160
SmlI	CTYRAG	2 cut(s)	131, 203
SmoI	CTYRAG	2 cut(s)	131, 203
SsiI	CCGC	7 cut(s)	36, 168, 256, 423, 826, 828, 906
SspDI	GGCGCC	3 cut(s)	50, 287, 326
SstI	GAGCTC	2 cut(s)	126, 867
StyD4I	CCNGG	5 cut(s)	21, 108, 208, 727, 1275
StyI	CCWWGG	3 cut(s)	216, 306, 969
TaaI	ACNGT	4 cut(s)	535, 856, 927, 1057
TaiI	ACGT	2 cut(s)	357, 919
TaqI	TCGA	6 cut(s)	90, 132, 204, 339, 351, 1094
TaqII	GACCGA	1 cut(s)	498
TatI	WGTACW	1 cut(s)	840
TauI	GCSGC	1 cut(s)	908
TfiI	GAWTC	1 cut(s)	10
Tru1I	TTAA	1 cut(s)	765
Tru9I	TTAA	1 cut(s)	765
TscAI	CASTG	5 cut(s)	38, 648, 1060, 1162, 1231
TseFI	GTSAC	1 cut(s)	407
TseI	GCWGC	1 cut(s)	714
Tsp45I	GTSAC	1 cut(s)	407
TspDTI	ATGAA	4 cut(s)	787, 1090, 1259, 1326
TspGWI	ACGGA	4 cut(s)	334, 493, 501, 941
TspRI	CASTG	5 cut(s)	38, 648, 1060, 1162, 1231
Tth111I	GACNNNGTC	4 cut(s)	224, 356, 430, 533
Van91I	CCANNNNNTGG	2 cut(s)	60, 920
VpaK11BI	GGWCC	2 cut(s)	432, 481
XhoI	CTCGAG	2 cut(s)	131, 203
XmiI	GTMKAC	1 cut(s)	147
ZraI	GACGTC	1 cut(s)	355

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.