MD02G1024300.v1.1

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0022381
Physical Location & Seq
Forward (+)
1809748 .. 1812719
2972 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1024300.v1.1.491

Sequence Viewer

Length: 957 bp
ATGAGGAGGAGGAAACTGAACAAGTATTCTTGTTGCTTAAGTTATGCAAGGACGTTGCTTACGTTATGCAAAGACGATGATAATATCGGTGATGAGGAAAGCAGTAGCTGCTCTCTTGTTGGTGTCCAGGACATCATTTTTCCTCACTCTTTTATTCAAAAGGTCCAGAAAGGCCATCATTTATTTCGATACAAGTTGCTAGGTATTTCATGGTATGCCGAACCATCTGAGCTCCAACGATTACCCCTGGGGCGTGACCTTGATTTGAAGCATATTGTGCGGAATGAATCAGTTGCCCTGTTTGCTCTTTTCTTTGAGAATCATCAGAATTATAGATATCCTAAACCTGACTTATATGAAATGTGGGTGACGGATGACGACTCTAACGGTGCTTGGACAAAACAGTTAACTATTGATGAGCGCACAGAGTGCCCTCCCGAGATGTTCCCACAAACATATCACATATTTTCTTCTAGGCCTGTGGCATTGTGGAGTGATGAGTTTCTTATGGTTCACAGTGATGAACGCTCAGTTTGCTTTAATCTTTGTACTACAAAGCTTACGTATATTCCCATTGAAACATTAGGTGAGTTTGTTGGTTATGTGAATGGCAGAGTTTCTGTCATGGGACGCAACCACAAGCCTGAGAGCATGGAGAATAATTGCTCAGACAAAGATGAAAATGCGATGGTGGTGGTAGACGGGAAGAGGGAAAGCTGCAGGAGGCAGTACACATATTCCGTTTCGGCCATTCAACCGGCTGATGTTTCCCATAGAATCAAGAAGGTGATGGAGGGCCTCCGGGCAGAGTTTGGTGCATGTCCCTGTAAACAGTTCTATGCCATATTAAGCCTCCTCTACGGGAAGTCGATGGAAGAAGAAGAGGAAAAAAAGCTCGAGAACTGGTTAATGCTCTGGATGGATGAGGGCATTACTAGCTTAACCCTGCGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

319

Amino Acids

37.04

Weight (kDa)

5.78

Isoelectric Point (pI)

53.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022381)

Species Orthologous Gene IDs
malus_domestica MD02G1023400.v1.1 MD02G1024300.v1.1 MD02G1024700.v1.1
pyrus_communis pycom02g02070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 699
AciI CCGC 1 cut(s) 280
AcoI YGGCCR 1 cut(s) 747
AdeI CACNNNGTG 1 cut(s) 429
AfaI GTAC 2 cut(s) 550, 731
AflII CTTAAG 1 cut(s) 37
AgsI TTSAA 4 cut(s) 158, 268, 578, 755
AjnI CCWGG 2 cut(s) 126, 246
AluBI AGCT 6 cut(s) 108, 232, 559, 717, 895, 939
AluI AGCT 6 cut(s) 108, 232, 559, 717, 895, 939
Alw21I GWGCWC 1 cut(s) 234
AlwNI CAGNNNCTG 1 cut(s) 108
Ama87I CYCGRG 2 cut(s) 437, 896
AoxI GGCC 4 cut(s) 172, 476, 747, 796
ApeKI GCWGC 2 cut(s) 108, 717
AspLEI GCGC 2 cut(s) 423, 951
AspS9I GGNCC 2 cut(s) 163, 796
AsuC2I CCSGG 1 cut(s) 803
AsuHPI GGTGA 4 cut(s) 101, 379, 599, 799
AvaI CYCGRG 2 cut(s) 437, 896
AvaII GGWCC 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 434
BanII GRGCYC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 2 cut(s) 95, 704
BccI CCATC 6 cut(s) 183, 232, 682, 784, 865, 913
BciT130I CCWGG 2 cut(s) 128, 248
BcnI CCSGG 1 cut(s) 803
BfaI CTAG 3 cut(s) 200, 474, 936
BfmI CTRYAG 1 cut(s) 718
BfrI CTTAAG 1 cut(s) 37
BisI GCNGC 2 cut(s) 109, 718
BlsI GCNGC 2 cut(s) 110, 719
Bme1390I CCNGG 3 cut(s) 128, 248, 803
Bme18I GGWCC 1 cut(s) 163
BmeT110I CYCGRG 2 cut(s) 437, 896
BmgT120I GGNCC 2 cut(s) 163, 796
BmrFI CCNGG 3 cut(s) 128, 248, 803
BpuMI CCSGG 1 cut(s) 803
BsaAI YACGTR 1 cut(s) 564
BsaJI CCNNGG 2 cut(s) 246, 247
Bse118I RCCGGY 1 cut(s) 757
Bse1I ACTGG 1 cut(s) 908
BseBI CCWGG 2 cut(s) 128, 248
BseDI CCNNGG 2 cut(s) 246, 247
BseGI GGATG 3 cut(s) 379, 924, 928
BseMII CTCAG 4 cut(s) 219, 543, 636, 681
BseNI ACTGG 1 cut(s) 908
BseRI GAGGAG 3 cut(s) 19, 22, 845
BseSI GKGCMC 1 cut(s) 434
BseXI GCAGC 2 cut(s) 95, 704
BshFI GGCC 4 cut(s) 174, 478, 749, 798
BsiHKAI GWGCWC 1 cut(s) 234
BsiHKCI CYCGRG 2 cut(s) 437, 896
BsiSI CCGG 2 cut(s) 758, 802
BslFI GGGAC 2 cut(s) 642, 807
BsmFI GGGAC 2 cut(s) 642, 807
BsnI GGCC 4 cut(s) 174, 478, 749, 798
BsoBI CYCGRG 2 cut(s) 437, 896
Bsp1286I GDGCHC 2 cut(s) 234, 434
BspACI CCGC 1 cut(s) 280
BspANI GGCC 4 cut(s) 174, 478, 749, 798
BspCNI CTCAG 4 cut(s) 220, 542, 637, 680
BspMAI CTGCAG 1 cut(s) 722
BspTI CTTAAG 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 757
BsrI ACTGG 1 cut(s) 908
BssAI RCCGGY 1 cut(s) 757
BssECI CCNNGG 2 cut(s) 246, 247
Bst2UI CCWGG 2 cut(s) 128, 248
Bst4CI ACNGT 4 cut(s) 389, 405, 518, 834
Bst6I CTCTTC 2 cut(s) 701, 876
BstAFI CTTAAG 1 cut(s) 37
BstAPI GCANNNNNTGC 3 cut(s) 108, 277, 429
BstBAI YACGTR 1 cut(s) 564
BstDEI CTNAG 4 cut(s) 228, 529, 645, 667
BstF5I GGATG 3 cut(s) 379, 924, 928
BstHHI GCGC 2 cut(s) 423, 951
BstMWI GCNNNNNNNGC 6 cut(s) 108, 277, 302, 429, 534, 936
BstNI CCWGG 2 cut(s) 128, 248
BstNSI RCATGY 1 cut(s) 822
BstSCI CCNGG 3 cut(s) 126, 246, 801
BstSFI CTRYAG 1 cut(s) 718
BstSLI GKGCMC 1 cut(s) 434
BstSNI TACGTA 1 cut(s) 564
BstV1I GCAGC 2 cut(s) 95, 704
BsuRI GGCC 4 cut(s) 174, 478, 749, 798
BtgZI GCGATG 1 cut(s) 701
BtsCI GGATG 3 cut(s) 379, 924, 928
BtsIMutI CAGTG 1 cut(s) 523
CaiI CAGNNNCTG 1 cut(s) 108
CfoI GCGC 2 cut(s) 423, 951
Cfr10I RCCGGY 1 cut(s) 757
Cfr13I GGNCC 2 cut(s) 163, 796
CseI GACGC 1 cut(s) 639
Csp6I GTAC 2 cut(s) 549, 730
CviAII CATG 4 cut(s) 210, 625, 652, 819
CviQI GTAC 2 cut(s) 549, 730
DdeI CTNAG 4 cut(s) 228, 529, 645, 667
DraIII CACNNNGTG 1 cut(s) 429
EaeI YGGCCR 1 cut(s) 747
Eam1104I CTCTTC 2 cut(s) 701, 876
EarI CTCTTC 2 cut(s) 701, 876
Ecl136II GAGCTC 1 cut(s) 232
Eco105I TACGTA 1 cut(s) 564
Eco147I AGGCCT 1 cut(s) 478
Eco24I GRGCYC 1 cut(s) 234
Eco32I GATATC 1 cut(s) 338
Eco47I GGWCC 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 232
Eco88I CYCGRG 2 cut(s) 437, 896
EcoICRI GAGCTC 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 796
EcoRII CCWGG 2 cut(s) 126, 246
EcoRV GATATC 1 cut(s) 338
EcoT38I GRGCYC 1 cut(s) 234
FaeI CATG 4 cut(s) 213, 628, 655, 822
FaqI GGGAC 2 cut(s) 642, 807
FatI CATG 4 cut(s) 209, 624, 651, 818
FblI GTMKAC 1 cut(s) 699
Fnu4HI GCNGC 2 cut(s) 109, 718
FokI GGATG 3 cut(s) 386, 931, 935
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 2 cut(s) 109, 718
FspBI CTAG 3 cut(s) 200, 474, 936
GlaI GCGC 2 cut(s) 422, 950
GluI GCNGC 2 cut(s) 109, 718
HaeIII GGCC 4 cut(s) 174, 478, 749, 798
HapII CCGG 2 cut(s) 758, 802
HgaI GACGC 1 cut(s) 639
HhaI GCGC 2 cut(s) 423, 951
Hin1II CATG 4 cut(s) 213, 628, 655, 822
Hin6I GCGC 2 cut(s) 421, 949
HinP1I GCGC 2 cut(s) 421, 949
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 4 cut(s) 287, 319, 380, 777
HpaI GTTAAC 1 cut(s) 408
HpaII CCGG 2 cut(s) 758, 802
HphI GGTGA 4 cut(s) 101, 379, 599, 799
Hpy166II GTNNAC 5 cut(s) 408, 514, 700, 732, 830
Hpy188I TCNGA 3 cut(s) 229, 327, 670
Hpy188III TCNNGA 5 cut(s) 166, 437, 781, 898, 916
Hpy8I GTNNAC 5 cut(s) 408, 514, 700, 732, 830
HpyAV CCTTC 1 cut(s) 778
HpyCH4III ACNGT 4 cut(s) 389, 405, 518, 834
HpyCH4IV ACGT 3 cut(s) 53, 62, 563
HpyCH4V TGCA 4 cut(s) 47, 69, 720, 818
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 277, 302, 429, 534, 936
HpyF3I CTNAG 4 cut(s) 228, 529, 645, 667
HpySE526I ACGT 3 cut(s) 53, 62, 563
Hsp92II CATG 4 cut(s) 213, 628, 655, 822
HspAI GCGC 2 cut(s) 421, 949
KspAI GTTAAC 1 cut(s) 408
LmnI GCTCC 1 cut(s) 237
Lsp1109I GCAGC 2 cut(s) 95, 704
MaeI CTAG 3 cut(s) 200, 474, 936
MaeII ACGT 3 cut(s) 53, 62, 563
MaeIII GTNAC 2 cut(s) 254, 367
MboII GAAGA 5 cut(s) 462, 718, 887, 890, 893
MhlI GDGCHC 2 cut(s) 234, 434
MluCI AATT 2 cut(s) 328, 661
MlyI GAGTC 1 cut(s) 374
MmeI TCCRAC 1 cut(s) 259
MseI TTAA 6 cut(s) 38, 407, 540, 848, 908, 941
MslI CAYNNNNRTG 1 cut(s) 519
MspCI CTTAAG 1 cut(s) 37
MspI CCGG 2 cut(s) 758, 802
MspR9I CCNGG 3 cut(s) 128, 248, 803
MvaI CCWGG 2 cut(s) 128, 248
MwoI GCNNNNNNNGC 6 cut(s) 108, 277, 302, 429, 534, 936
NciI CCSGG 1 cut(s) 803
NlaIII CATG 4 cut(s) 213, 628, 655, 822
NmuCI GTSAC 2 cut(s) 254, 367
NspI RCATGY 1 cut(s) 822
PaeR7I CTCGAG 1 cut(s) 896
PasI CCCWGGG 1 cut(s) 247
PceI AGGCCT 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PfeI GAWTC 3 cut(s) 287, 319, 777
PfoI TCCNGGA 1 cut(s) 126
PkrI GCNGC 2 cut(s) 110, 719
PleI GAGTC 1 cut(s) 374
PpsI GAGTC 1 cut(s) 374
Ppu21I YACGTR 1 cut(s) 564
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 126, 246
PspGI CCWGG 2 cut(s) 126, 246
PspPI GGNCC 2 cut(s) 163, 796
PstI CTGCAG 1 cut(s) 722
PstNI CAGNNNCTG 1 cut(s) 108
RsaI GTAC 2 cut(s) 550, 731
RsaNI GTAC 2 cut(s) 549, 730
RseI CAYNNNNRTG 1 cut(s) 519
SacI GAGCTC 1 cut(s) 234
SaqAI TTAA 6 cut(s) 38, 407, 540, 848, 908, 941
SatI GCNGC 2 cut(s) 109, 718
Sau96I GGNCC 2 cut(s) 163, 796
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 3 cut(s) 128, 248, 803
SduI GDGCHC 2 cut(s) 234, 434
SfcI CTRYAG 1 cut(s) 718
Sfr274I CTCGAG 1 cut(s) 896
SinI GGWCC 1 cut(s) 163
SlaI CTCGAG 1 cut(s) 896
SmiMI CAYNNNNRTG 1 cut(s) 519
SmlI CTYRAG 2 cut(s) 37, 896
SmoI CTYRAG 2 cut(s) 37, 896
SnaBI TACGTA 1 cut(s) 564
Sse9I AATT 2 cut(s) 328, 661
SseBI AGGCCT 1 cut(s) 478
SsiI CCGC 1 cut(s) 280
SspMI CTAG 3 cut(s) 200, 474, 936
SstI GAGCTC 1 cut(s) 234
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 3 cut(s) 126, 246, 801
TaaI ACNGT 4 cut(s) 389, 405, 518, 834
TaiI ACGT 3 cut(s) 56, 65, 566
TaqI TCGA 3 cut(s) 187, 869, 897
TasI AATT 2 cut(s) 328, 661
TatI WGTACW 2 cut(s) 548, 729
TfiI GAWTC 3 cut(s) 287, 319, 777
Tru1I TTAA 6 cut(s) 38, 407, 540, 848, 908, 941
Tru9I TTAA 6 cut(s) 38, 407, 540, 848, 908, 941
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 2 cut(s) 254, 367
TseI GCWGC 2 cut(s) 108, 717
Tsp45I GTSAC 2 cut(s) 254, 367
TspDTI ATGAA 5 cut(s) 198, 300, 372, 537, 693
TspGWI ACGGA 2 cut(s) 386, 730
TspRI CASTG 1 cut(s) 523
Vha464I CTTAAG 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 163
XceI RCATGY 1 cut(s) 822
XhoI CTCGAG 1 cut(s) 896
XmiI GTMKAC 1 cut(s) 699
XspI CTAG 3 cut(s) 200, 474, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.