MD02G1024700.v1.1

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0022381
Physical Location & Seq
Forward (+)
1830902 .. 1831478
577 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1024700.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGTTCACAGTGATGAACGCTCAGTTTGCTTTAATCTTTGTACTACAAAGCTTACGTATATTCCCATTGAAACATTAGGTGAGTTTGTTGGTTATGTGAATAGCAGAGTTTCTGTCATGGGACGCAACCACAAGCCTGAGAGCATGGAGAATAATTGCTCAGACAAAGATGAAGATGCGATGGTGGTGGTAGACGGGAAGAGGGAAAGCTGCAGGAGGCGGTACACATATTCCGTTTCGGCCATTCAACCGGCTGATGTTTCCCATAGAATCAAGAAGGTGATGGAGGGCCTCCGGGCAGAGTTTGGTGCATGTCCCTGTAAACAGTTCTATGACATATTTAAGCCTCCTCTACTGGAAGTCGATGGATGCAGGTTCTATGCCATATGTCAGAAACAAGTTTTTCTTGCAAATCAACCAGACCATGTTTACACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

146

Amino Acids

16.6

Weight (kDa)

6.88

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022381)

Species Orthologous Gene IDs
malus_domestica MD02G1023400.v1.1 MD02G1024300.v1.1 MD02G1024700.v1.1
pyrus_communis pycom02g02070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 363
AccI GTMKAC 1 cut(s) 192
AciI CCGC 1 cut(s) 220
AcoI YGGCCR 1 cut(s) 240
AfaI GTAC 2 cut(s) 43, 224
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 2 cut(s) 71, 248
AluBI AGCT 2 cut(s) 52, 210
AluI AGCT 2 cut(s) 52, 210
AoxI GGCC 2 cut(s) 240, 289
ApeKI GCWGC 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 289
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 92, 292
BbvI GCAGC 1 cut(s) 197
BccI CCATC 3 cut(s) 175, 277, 359
BcnI CCSGG 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 211
BfuAI ACCTGC 1 cut(s) 363
BisI GCNGC 1 cut(s) 211
BlsI GCNGC 1 cut(s) 212
Bme1390I CCNGG 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 289
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 2 cut(s) 166, 359
BpuMI CCSGG 1 cut(s) 296
BsaAI YACGTR 2 cut(s) 57, 435
Bse118I RCCGGY 1 cut(s) 250
Bse1I ACTGG 1 cut(s) 360
BseGI GGATG 1 cut(s) 374
BseMII CTCAG 3 cut(s) 36, 129, 174
BseNI ACTGG 1 cut(s) 360
BseRI GAGGAG 1 cut(s) 339
BseXI GCAGC 1 cut(s) 197
BshFI GGCC 2 cut(s) 242, 291
BsiSI CCGG 2 cut(s) 251, 295
BslFI GGGAC 2 cut(s) 135, 300
BsmFI GGGAC 2 cut(s) 135, 300
BsnI GGCC 2 cut(s) 242, 291
BspACI CCGC 1 cut(s) 220
BspANI GGCC 2 cut(s) 242, 291
BspCNI CTCAG 3 cut(s) 35, 130, 173
BspMAI CTGCAG 1 cut(s) 215
BspMI ACCTGC 1 cut(s) 363
BsrFI RCCGGY 1 cut(s) 250
BsrI ACTGG 1 cut(s) 360
BssAI RCCGGY 1 cut(s) 250
Bst4CI ACNGT 2 cut(s) 11, 327
Bst6I CTCTTC 1 cut(s) 194
BstBAI YACGTR 2 cut(s) 57, 435
BstDEI CTNAG 3 cut(s) 22, 138, 160
BstF5I GGATG 1 cut(s) 374
BstMWI GCNNNNNNNGC 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 1 cut(s) 211
BstSNI TACGTA 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 197
BsuRI GGCC 2 cut(s) 242, 291
BtgZI GCGATG 1 cut(s) 194
BtsCI GGATG 1 cut(s) 374
BtsIMutI CAGTG 1 cut(s) 16
BveI ACCTGC 1 cut(s) 363
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 289
CseI GACGC 1 cut(s) 132
Csp6I GTAC 2 cut(s) 42, 223
CviAII CATG 4 cut(s) 118, 145, 312, 425
CviJI RGCY 7 cut(s) 52, 136, 210, 242, 254, 291, 346
CviKI_1 RGCY 7 cut(s) 52, 136, 210, 242, 254, 291, 346
CviQI GTAC 2 cut(s) 42, 223
DdeI CTNAG 3 cut(s) 22, 138, 160
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
Eco105I TACGTA 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 289
FaeI CATG 4 cut(s) 121, 148, 315, 428
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 2 cut(s) 135, 300
FatI CATG 4 cut(s) 117, 144, 311, 424
FauNDI CATATG 1 cut(s) 386
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 1 cut(s) 211
FokI GGATG 1 cut(s) 381
Fsp4HI GCNGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 211
HaeIII GGCC 2 cut(s) 242, 291
HapII CCGG 2 cut(s) 251, 295
HgaI GACGC 1 cut(s) 132
Hin1II CATG 4 cut(s) 121, 148, 315, 428
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 1 cut(s) 270
HpaII CCGG 2 cut(s) 251, 295
HphI GGTGA 2 cut(s) 92, 292
Hpy166II GTNNAC 5 cut(s) 7, 193, 225, 323, 430
Hpy188I TCNGA 2 cut(s) 163, 393
Hpy188III TCNNGA 1 cut(s) 274
Hpy8I GTNNAC 5 cut(s) 7, 193, 225, 323, 430
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 2 cut(s) 11, 327
HpyCH4IV ACGT 2 cut(s) 56, 434
HpyCH4V TGCA 4 cut(s) 213, 311, 372, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 27
HpyF3I CTNAG 3 cut(s) 22, 138, 160
HpySE526I ACGT 2 cut(s) 56, 434
Hsp92II CATG 4 cut(s) 121, 148, 315, 428
LpnPI CCDG 8 cut(s) 150, 199, 264, 308, 331, 341, 358, 432
Lsp1109I GCAGC 1 cut(s) 197
LweI GCATC 2 cut(s) 166, 359
MaeII ACGT 2 cut(s) 56, 434
MboII GAAGA 2 cut(s) 185, 211
MluCI AATT 1 cut(s) 154
MnlI CCTC 6 cut(s) 195, 210, 280, 302, 357, 360
MseI TTAA 2 cut(s) 33, 342
MslI CAYNNNNRTG 1 cut(s) 12
MspI CCGG 2 cut(s) 251, 295
MspR9I CCNGG 1 cut(s) 296
MwoI GCNNNNNNNGC 1 cut(s) 27
NciI CCSGG 1 cut(s) 296
NdeI CATATG 1 cut(s) 386
NlaIII CATG 4 cut(s) 121, 148, 315, 428
NspI RCATGY 1 cut(s) 315
PfeI GAWTC 1 cut(s) 270
PkrI GCNGC 1 cut(s) 212
Ppu21I YACGTR 2 cut(s) 57, 435
PspPI GGNCC 1 cut(s) 289
PstI CTGCAG 1 cut(s) 215
RsaI GTAC 2 cut(s) 43, 224
RsaNI GTAC 2 cut(s) 42, 223
RseI CAYNNNNRTG 1 cut(s) 12
SaqAI TTAA 2 cut(s) 33, 342
SatI GCNGC 1 cut(s) 211
Sau96I GGNCC 1 cut(s) 289
ScrFI CCNGG 1 cut(s) 296
SetI ASST 7 cut(s) 54, 59, 82, 212, 282, 377, 437
SfaNI GCATC 2 cut(s) 166, 359
SfcI CTRYAG 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 12
SnaBI TACGTA 1 cut(s) 57
Sse9I AATT 1 cut(s) 154
SsiI CCGC 1 cut(s) 220
StyD4I CCNGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 11, 327
TaiI ACGT 2 cut(s) 59, 437
TaqI TCGA 1 cut(s) 363
TasI AATT 1 cut(s) 154
TatI WGTACW 1 cut(s) 41
TfiI GAWTC 1 cut(s) 270
Tru1I TTAA 2 cut(s) 33, 342
Tru9I TTAA 2 cut(s) 33, 342
TscAI CASTG 1 cut(s) 16
TseI GCWGC 1 cut(s) 210
TspDTI ATGAA 2 cut(s) 30, 186
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 1 cut(s) 16
XceI RCATGY 1 cut(s) 315
XmiI GTMKAC 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.