Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011494

Physical Location & Seq

Genomic Coordinates Reverse (-)

3674784 .. 3678856

Gene Structure 4073 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1045900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1554 bp

ATGGCCGTGGTTGCGAGGCTTCACGCATGCCCACAGAGCTTGGAGTACTTCAACTTCAAGGACCACCGCGGAACCGGCCCGGCGGTGGTAATCGGGTCGGGGTTTCGGCGTTCCGGGGGTCTGCTGGCGGGGAGAAATGGGTTTTGCTTGAAACCGTGCAGATCGTTTAGGGGGGAGGATAACGGAGGGGATGCGGAGGAGGGAGAGGCAGAAAGAGCGAGAAAGCGTGGGAATTTGAAGAAGGGAGGTGGGATTTTGAAATCTTTGAAATCTTCGGTTTGGGGTGGTTTTGGTTTGGGGTTTAGAGATGGGGATGAACACAGGAAGGGTGTGGCCAAGTTGGAGGAGATCTGTCAATCTGCTGCTGTTCAAATCGGAAGATATATTGTGACCATGATGAGCACAGGCGTTATACTAGCAATTGGGTTTCAGTTGTCAGGTGGAGACGCTGATTTGAATGAGCTAGTTTGGTATAGCTGGCTTGGTGGAATTATTATCGGAACTATGGTTGGAGCTAACATGGTTTTAGAGGATCACTGTCGAGCTGGTCCACGAAATGTTGTCATCAGTGGAAGCACAAGGGGACTTGGAAAAGCACTTGCTCGTGAATTTCTTCTTTCTGGAGATCGTGTGGTTGTTGCTTCCCGAAGCCCTGCGTCTGTAGAAGCAACTGTCAGAGAGCTCGAGGAAAACCTAAAGGAAGGTATAAACAGTGCAGGCGGCTTATCCAAGAACCTGAAACATGCAAAAGTGGTTGGCATAGCATGTGATGTTTGTGAACCTGGTGATGTGAAGAAACTGGCAAATTTTGCTGTTAGTGAACTCGGTCATATTGACATTTGGATAAACAATGCTGGTGCTAATAAAGGATTTAGACCACTGCTTCAGTTTACAGACGAAGACATTAAGCAGATTGTCTCGACAAACCTGGTTGGTTCTATACTCTGCACTCGAGAAGCGATGCATATAATGATGAATCAGCCTAAAGGTGGGCACATATTTAACATGGATGGAGCAGGCTCTGGGGGATCAAGTACTCCTTTGACAGCAGTCTATGGATCGACAAAGTGTGGTCTTAGACAACTTCAATCATCACTTTTAAAGGAGTGCAAGCGGTCCAAAGTTGGAGTACACACAGCATCTCCTGGCATGGTTCTTACCGATCTTCTTCTGAGTGGTTCTTCCTTGAAGAATAAACAAATGTTTAACTTCATTTGTGAGCTTCCTGAAACAGTAGCAAGAACTCTGGTTCCACGGATGCGAGTTGTAAAGGGAACAGGAAAGGCCATCAATTACTTGACACCTCCTAGGATCTTACTCGCCTTAGTAACTGCATGGCTGCGACGAGGTCGCTGGTTTGATGACCAGGGCAAGGCATTATACGCTGCAGAAGCAGACAGACTTCGGAACTGGGCAGAAAACCGCACCCGTTTTTACTTCACTGATGCAATGGAGATGTACACGGAGAACACATGGGTGTCTGTCTTCTCACTTTCCGTTGTTTGCGCCTTCATAATTCTTTCTAGCACCAGCAGCAATTTGCCCGGTACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006778 GO:0006787 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0010304 GO:0015994 GO:0015996 GO:0016491 GO:0016614 GO:0016616 GO:0019439 GO:0019538 GO:0030163 GO:0033013 GO:0033015 GO:0034256 GO:0034641 GO:0042440 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046149 GO:0046483 GO:0046700 GO:0051186 GO:0051187 GO:0055114 GO:0071704 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 map00860 map01100 map01110

Pfam Domains

Protein Families

adh_short

Protein Analysis

518

Amino Acids

55.81

Weight (kDa)

9.3

Isoelectric Point (pI)

39.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	186 - 389	8.6e-35	short chain dehydrogenase
adh_short_C2	PF13561	193 - 392	2.1e-24	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13250 AT4G13250
fragaria_vesca	FvH4_1g04700
malus_domestica	MD02G1045900.v1.1 MD15G1184100.v1.1
prunus_persica	Prupe.7G232100_v2.0.a1 Prupe.7G232100_v2.0.a1 Prupe.7G232100_v2.0.a1 Prupe.7G232100_v2.0.a1
pyrus_communis	pycom02g03830 pycom15g16570
rosa_chinensis	RchiOBHm_Chr2g0090301
rosa_laevigata	RLG00000016096
rosa_multiflora	Rmu_sc0000436.1_g000005 Rmu_sc0006235.1_g000003
rosa_roxburghii	Rroxscaffold_2G00151300
rosa_rugosa	Rorug02G0006400
rosa_samantha	Rh2BG050900 Rh2CG053100
rosa_wichuraiana	Rw2G004460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	69
AciI	CCGC	8 cut(s)	67, 69, 83, 128, 194, 720, 1114, 1423
AclWI	GGATC	4 cut(s)	540, 1036, 1066, 1319
AcoI	YGGCCR	2 cut(s)	3, 333
AcsI	RAATTY	3 cut(s)	232, 608, 805
AcuI	CTGAAG	1 cut(s)	869
AfaI	GTAC	5 cut(s)	47, 1036, 1131, 1460, 1549
AfiI	CCNNNNNNNGG	3 cut(s)	85, 989, 1372
AjnI	CCWGG	4 cut(s)	781, 927, 1144, 1365
AleI	CACNNNNGTG	1 cut(s)	1475
AloI	GAACNNNNNNTCC	2 cut(s)	1234, 1266
AluBI	AGCT	7 cut(s)	39, 463, 477, 515, 545, 682, 1222
AluI	AGCT	7 cut(s)	39, 463, 477, 515, 545, 682, 1222
Alw21I	GWGCWC	2 cut(s)	404, 684
Alw26I	GTCTC	2 cut(s)	438, 922
AlwI	GGATC	4 cut(s)	540, 1036, 1066, 1319
AlwNI	CAGNNNCTG	1 cut(s)	1022
Ama87I	CYCGRG	2 cut(s)	683, 951
AoxI	GGCC	4 cut(s)	3, 76, 333, 1284
ApeKI	GCWGC	4 cut(s)	362, 1339, 1385, 1533
ApoI	RAATTY	3 cut(s)	232, 608, 805
Asp700I	GAANNNNTTC	1 cut(s)	612
AspA2I	CCTAGG	1 cut(s)	1307
AspLEI	GCGC	1 cut(s)	1508
AspS9I	GGNCC	4 cut(s)	61, 77, 548, 1116
AsuC2I	CCSGG	3 cut(s)	80, 115, 1545
AsuHPI	GGTGA	1 cut(s)	797
AvaI	CYCGRG	2 cut(s)	683, 951
AvaII	GGWCC	3 cut(s)	61, 548, 1116
AvrII	CCTAGG	1 cut(s)	1307
BaeGI	GKGCMC	1 cut(s)	996
BalI	TGGCCA	1 cut(s)	335
BanII	GRGCYC	1 cut(s)	684
BarI	GAAGNNNNNNTAC	2 cut(s)	38, 70
BauI	CACGAG	1 cut(s)	603
BbsI	GAAGAC	2 cut(s)	906, 1477
Bbv12I	GWGCWC	2 cut(s)	404, 684
BbvI	GCAGC	4 cut(s)	349, 1326, 1372, 1545
BccI	CCATC	3 cut(s)	302, 1004, 1295
BciT130I	CCWGG	4 cut(s)	783, 929, 1146, 1367
BcnI	CCSGG	3 cut(s)	80, 115, 1545
BcoDI	GTCTC	2 cut(s)	438, 922
BfaI	CTAG	4 cut(s)	416, 464, 1308, 1524
BfmI	CTRYAG	2 cut(s)	660, 1386
BglII	AGATCT	1 cut(s)	348
BisI	GCNGC	5 cut(s)	363, 721, 1340, 1386, 1534
BlnI	CCTAGG	1 cut(s)	1307
BlsI	GCNGC	5 cut(s)	364, 722, 1341, 1387, 1535
BmcAI	AGTACT	2 cut(s)	47, 1036
Bme1390I	CCNGG	7 cut(s)	80, 115, 783, 929, 1146, 1367, 1545
Bme18I	GGWCC	3 cut(s)	61, 548, 1116
BmeT110I	CYCGRG	2 cut(s)	683, 951
BmgT120I	GGNCC	4 cut(s)	61, 77, 548, 1116
BmiI	GGNNCC	2 cut(s)	73, 1251
BmrFI	CCNGG	7 cut(s)	80, 115, 783, 929, 1146, 1367, 1545
BmrI	ACTGGG	1 cut(s)	1420
BmsI	GCATC	5 cut(s)	181, 951, 1148, 1248, 1435
BmuI	ACTGGG	1 cut(s)	1420
BoxI	GACNNNNGTC	1 cut(s)	1049
BpiI	GAAGAC	2 cut(s)	906, 1477
BpmI	CTGGAG	1 cut(s)	642
BpuMI	CCSGG	3 cut(s)	80, 115, 1545
BsaJI	CCNNGG	6 cut(s)	6, 67, 114, 1253, 1307, 1366
BsaXI	ACNNNNNCTCC	4 cut(s)	615, 645, 1126, 1156
Bsc4I	CCNNNNNNNGG	3 cut(s)	85, 989, 1372
Bse118I	RCCGGY	1 cut(s)	74
Bse1I	ACTGG	2 cut(s)	804, 1415
Bse3DI	GCAATG	1 cut(s)	1455
BseBI	CCWGG	4 cut(s)	783, 929, 1146, 1367
BseDI	CCNNGG	6 cut(s)	6, 67, 114, 1253, 1307, 1366
BseGI	GGATG	4 cut(s)	196, 319, 1015, 1263
BseLI	CCNNNNNNNGG	3 cut(s)	85, 989, 1372
BseMI	GCAATG	1 cut(s)	1455
BseMII	CTCAG	1 cut(s)	1163
BseNI	ACTGG	2 cut(s)	804, 1415
BseRI	GAGGAG	2 cut(s)	212, 359
BseSI	GKGCMC	1 cut(s)	996
BseXI	GCAGC	4 cut(s)	349, 1326, 1372, 1545
BsgI	GTGCAG	3 cut(s)	178, 735, 931
Bsh1236I	CGCG	1 cut(s)	69
BshFI	GGCC	4 cut(s)	5, 78, 335, 1286
BsiHKAI	GWGCWC	2 cut(s)	404, 684
BsiHKCI	CYCGRG	2 cut(s)	683, 951
BsiSI	CCGG	4 cut(s)	75, 80, 114, 1545
BslFI	GGGAC	1 cut(s)	597
BslI	CCNNNNNNNGG	3 cut(s)	85, 989, 1372
BsmAI	GTCTC	2 cut(s)	438, 922
BsmBI	CGTCTC	1 cut(s)	438
BsmFI	GGGAC	1 cut(s)	597
BsnI	GGCC	4 cut(s)	5, 78, 335, 1286
BsoBI	CYCGRG	2 cut(s)	683, 951
Bsp1286I	GDGCHC	3 cut(s)	404, 684, 996
Bsp1407I	TGTACA	1 cut(s)	1458
Bsp143I	GATC	8 cut(s)	161, 348, 532, 625, 1028, 1058, 1162, 1311
BspACI	CCGC	8 cut(s)	67, 69, 83, 128, 194, 720, 1114, 1423
BspANI	GGCC	4 cut(s)	5, 78, 335, 1286
BspCNI	CTCAG	1 cut(s)	1164
BspFNI	CGCG	1 cut(s)	69
BspLI	GGNNCC	2 cut(s)	73, 1251
BspMAI	CTGCAG	1 cut(s)	1390
BspPI	GGATC	4 cut(s)	540, 1036, 1066, 1319
BsrDI	GCAATG	1 cut(s)	1455
BsrFI	RCCGGY	1 cut(s)	74
BsrGI	TGTACA	1 cut(s)	1458
BsrI	ACTGG	2 cut(s)	804, 1415
BssAI	RCCGGY	1 cut(s)	74
BssECI	CCNNGG	6 cut(s)	6, 67, 114, 1253, 1307, 1366
BssMI	GATC	8 cut(s)	161, 348, 532, 625, 1028, 1058, 1162, 1311
BssSI	CACGAG	1 cut(s)	603
BssT1I	CCWWGG	1 cut(s)	1307
Bst2BI	CACGAG	1 cut(s)	603
Bst2UI	CCWGG	4 cut(s)	783, 929, 1146, 1367
Bst4CI	ACNGT	5 cut(s)	156, 539, 673, 713, 1234
BstAPI	GCANNNNNTGC	1 cut(s)	809
BstAUI	TGTACA	1 cut(s)	1458
BstC8I	GCNNGC	6 cut(s)	28, 126, 479, 718, 1018, 1112
BstDEI	CTNAG	3 cut(s)	1076, 1172, 1324
BstDSI	CCRYGG	3 cut(s)	6, 67, 1253
BstF5I	GGATG	4 cut(s)	196, 319, 1015, 1263
BstFNI	CGCG	1 cut(s)	69
BstHHI	GCGC	1 cut(s)	1508
BstKTI	GATC	8 cut(s)	164, 351, 535, 628, 1031, 1061, 1165, 1314
BstMAI	GTCTC	2 cut(s)	438, 922
BstMBI	GATC	8 cut(s)	161, 348, 532, 625, 1028, 1058, 1162, 1311
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 36, 75, 215, 809, 1382, 1391, 1533
BstNI	CCWGG	4 cut(s)	783, 929, 1146, 1367
BstNSI	RCATGY	3 cut(s)	30, 746, 768
BstPAI	GACNNNNGTC	1 cut(s)	1049
BstSCI	CCNGG	7 cut(s)	78, 113, 781, 927, 1144, 1365, 1543
BstSFI	CTRYAG	2 cut(s)	660, 1386
BstSLI	GKGCMC	1 cut(s)	996
BstUI	CGCG	1 cut(s)	69
BstV1I	GCAGC	4 cut(s)	349, 1326, 1372, 1545
BstV2I	GAAGAC	2 cut(s)	906, 1477
BstX2I	RGATCY	2 cut(s)	348, 1311
BstYI	RGATCY	2 cut(s)	348, 1311
BsuRI	GGCC	4 cut(s)	5, 78, 335, 1286
BtgI	CCRYGG	3 cut(s)	6, 67, 1253
BtgZI	GCGATG	1 cut(s)	974
BtsCI	GGATG	4 cut(s)	196, 319, 1015, 1263
BtsI	GCAGTG	1 cut(s)	878
BtsIMutI	CAGTG	5 cut(s)	535, 574, 718, 878, 1440
Cac8I	GCNNGC	6 cut(s)	28, 126, 479, 718, 1018, 1112
CaiI	CAGNNNCTG	1 cut(s)	1022
CfoI	GCGC	1 cut(s)	1508
Cfr10I	RCCGGY	1 cut(s)	74
Cfr13I	GGNCC	4 cut(s)	61, 77, 548, 1116
Cfr42I	CCGCGG	1 cut(s)	70
CseI	GACGC	2 cut(s)	455, 645
CsiI	ACCWGGT	2 cut(s)	781, 927
Csp6I	GTAC	5 cut(s)	46, 1035, 1130, 1459, 1548
CspCI	CAANNNNNGTGG	2 cut(s)	21, 56
CviAII	CATG	9 cut(s)	27, 394, 520, 743, 765, 1006, 1150, 1335, 1473
CviQI	GTAC	5 cut(s)	46, 1035, 1130, 1459, 1548
DdeI	CTNAG	3 cut(s)	1076, 1172, 1324
DpnI	GATC	8 cut(s)	163, 350, 534, 627, 1030, 1060, 1164, 1313
DpnII	GATC	8 cut(s)	161, 348, 532, 625, 1028, 1058, 1162, 1311
DraI	TTTAAA	1 cut(s)	1101
EaeI	YGGCCR	2 cut(s)	3, 333
Ecl136II	GAGCTC	1 cut(s)	682
Eco130I	CCWWGG	1 cut(s)	1307
Eco24I	GRGCYC	1 cut(s)	684
Eco47I	GGWCC	3 cut(s)	61, 548, 1116
Eco53kI	GAGCTC	1 cut(s)	682
Eco57I	CTGAAG	1 cut(s)	869
Eco88I	CYCGRG	2 cut(s)	683, 951
EcoICRI	GAGCTC	1 cut(s)	682
EcoRII	CCWGG	4 cut(s)	781, 927, 1144, 1365
EcoT14I	CCWWGG	1 cut(s)	1307
EcoT22I	ATGCAT	1 cut(s)	966
EcoT38I	GRGCYC	1 cut(s)	684
ErhI	CCWWGG	1 cut(s)	1307
Esp3I	CGTCTC	1 cut(s)	438
FaeI	CATG	9 cut(s)	30, 397, 523, 746, 768, 1009, 1153, 1338, 1476
FalI	AAGNNNNNCTT	2 cut(s)	1024, 1056
FaqI	GGGAC	1 cut(s)	597
FatI	CATG	9 cut(s)	26, 393, 519, 742, 764, 1005, 1149, 1334, 1472
FauI	CCCGC	1 cut(s)	121
Fnu4HI	GCNGC	5 cut(s)	363, 721, 1340, 1386, 1534
FokI	GGATG	4 cut(s)	203, 326, 1022, 1270
FriOI	GRGCYC	1 cut(s)	684
Fsp4HI	GCNGC	5 cut(s)	363, 721, 1340, 1386, 1534
FspBI	CTAG	4 cut(s)	416, 464, 1308, 1524
GlaI	GCGC	1 cut(s)	1507
GluI	GCNGC	5 cut(s)	363, 721, 1340, 1386, 1534
GsuI	CTGGAG	1 cut(s)	642
HaeIII	GGCC	4 cut(s)	5, 78, 335, 1286
HapII	CCGG	4 cut(s)	75, 80, 114, 1545
HgaI	GACGC	2 cut(s)	455, 645
HhaI	GCGC	1 cut(s)	1508
Hin1II	CATG	9 cut(s)	30, 397, 523, 746, 768, 1009, 1153, 1338, 1476
Hin6I	GCGC	1 cut(s)	1506
HinP1I	GCGC	1 cut(s)	1506
HinfI	GANTC	1 cut(s)	976
HpaII	CCGG	4 cut(s)	75, 80, 114, 1545
HphI	GGTGA	1 cut(s)	797
Hpy166II	GTNNAC	6 cut(s)	551, 779, 821, 891, 1132, 1461
Hpy188I	TCNGA	5 cut(s)	377, 500, 677, 1173, 1407
Hpy188III	TCNNGA	6 cut(s)	605, 621, 645, 919, 953, 1226
Hpy8I	GTNNAC	6 cut(s)	551, 779, 821, 891, 1132, 1461
Hpy99I	CGWCG	1 cut(s)	1347
HpyAV	CCTTC	4 cut(s)	235, 319, 695, 1519
HpyCH4III	ACNGT	5 cut(s)	156, 539, 673, 713, 1234
HpyCH4V	TGCA	9 cut(s)	159, 716, 746, 948, 964, 1110, 1334, 1388, 1448
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 36, 75, 215, 809, 1382, 1391, 1533
HpyF3I	CTNAG	3 cut(s)	1076, 1172, 1324
Hsp92II	CATG	9 cut(s)	30, 397, 523, 746, 768, 1009, 1153, 1338, 1476
HspAI	GCGC	1 cut(s)	1506
KspI	CCGCGG	1 cut(s)	70
Kzo9I	GATC	8 cut(s)	161, 348, 532, 625, 1028, 1058, 1162, 1311
LmnI	GCTCC	2 cut(s)	512, 1013
Lsp1109I	GCAGC	4 cut(s)	349, 1326, 1372, 1545
LweI	GCATC	5 cut(s)	181, 951, 1148, 1248, 1435
MabI	ACCWGGT	2 cut(s)	781, 927
MaeI	CTAG	4 cut(s)	416, 464, 1308, 1524
MaeIII	GTNAC	2 cut(s)	388, 1327
MalI	GATC	8 cut(s)	163, 350, 534, 627, 1030, 1060, 1164, 1313
MboI	GATC	8 cut(s)	161, 348, 532, 625, 1028, 1058, 1162, 1311
MfeI	CAATTG	1 cut(s)	420
MflI	RGATCY	2 cut(s)	348, 1311
MhlI	GDGCHC	3 cut(s)	404, 684, 996
MlsI	TGGCCA	1 cut(s)	335
MluCI	AATT	8 cut(s)	232, 420, 489, 608, 805, 1291, 1515, 1537
MluNI	TGGCCA	1 cut(s)	335
MmeI	TCCRAC	3 cut(s)	321, 490, 1105
Mox20I	TGGCCA	1 cut(s)	335
Mph1103I	ATGCAT	1 cut(s)	966
MroXI	GAANNNNTTC	1 cut(s)	612
MscI	TGGCCA	1 cut(s)	335
MseI	TTAA	5 cut(s)	906, 1002, 1100, 1206, 1552
MslI	CAYNNNNRTG	1 cut(s)	1475
Msp20I	TGGCCA	1 cut(s)	335
MspA1I	CMGCKG	1 cut(s)	69
MspI	CCGG	4 cut(s)	75, 80, 114, 1545
MspR9I	CCNGG	7 cut(s)	80, 115, 783, 929, 1146, 1367, 1545
MunI	CAATTG	1 cut(s)	420
MvaI	CCWGG	4 cut(s)	783, 929, 1146, 1367
MvnI	CGCG	1 cut(s)	69
MwoI	GCNNNNNNNGC	8 cut(s)	11, 36, 75, 215, 809, 1382, 1391, 1533
NciI	CCSGG	3 cut(s)	80, 115, 1545
NdeII	GATC	8 cut(s)	161, 348, 532, 625, 1028, 1058, 1162, 1311
NlaIII	CATG	9 cut(s)	30, 397, 523, 746, 768, 1009, 1153, 1338, 1476
NlaIV	GGNNCC	2 cut(s)	73, 1251
NmuCI	GTSAC	1 cut(s)	388
NsiI	ATGCAT	1 cut(s)	966
NspI	RCATGY	3 cut(s)	30, 746, 768
OliI	CACNNNNGTG	1 cut(s)	1475
PaeI	GCATGC	1 cut(s)	30
PaeR7I	CTCGAG	2 cut(s)	683, 951
PdmI	GAANNNNTTC	1 cut(s)	612
PfeI	GAWTC	1 cut(s)	976
PflFI	GACNNNGTC	1 cut(s)	1347
PkrI	GCNGC	5 cut(s)	364, 722, 1341, 1387, 1535
PshAI	GACNNNNGTC	1 cut(s)	1049
Psp124BI	GAGCTC	1 cut(s)	684
Psp6I	CCWGG	4 cut(s)	781, 927, 1144, 1365
PspGI	CCWGG	4 cut(s)	781, 927, 1144, 1365
PspN4I	GGNNCC	2 cut(s)	73, 1251
PspPI	GGNCC	4 cut(s)	61, 77, 548, 1116
PspXI	VCTCGAGB	1 cut(s)	683
PstI	CTGCAG	1 cut(s)	1390
PstNI	CAGNNNCTG	1 cut(s)	1022
PsuI	RGATCY	2 cut(s)	348, 1311
PsyI	GACNNNGTC	1 cut(s)	1347
RsaI	GTAC	5 cut(s)	47, 1036, 1131, 1460, 1549
RsaNI	GTAC	5 cut(s)	46, 1035, 1130, 1459, 1548
RseI	CAYNNNNRTG	1 cut(s)	1475
SacI	GAGCTC	1 cut(s)	684
SacII	CCGCGG	1 cut(s)	70
SaqAI	TTAA	5 cut(s)	906, 1002, 1100, 1206, 1552
SatI	GCNGC	5 cut(s)	363, 721, 1340, 1386, 1534
Sau3AI	GATC	8 cut(s)	161, 348, 532, 625, 1028, 1058, 1162, 1311
Sau96I	GGNCC	4 cut(s)	61, 77, 548, 1116
ScaI	AGTACT	2 cut(s)	47, 1036
ScrFI	CCNGG	7 cut(s)	80, 115, 783, 929, 1146, 1367, 1545
SduI	GDGCHC	3 cut(s)	404, 684, 996
SexAI	ACCWGGT	2 cut(s)	781, 927
SfaNI	GCATC	5 cut(s)	181, 951, 1148, 1248, 1435
SfcI	CTRYAG	2 cut(s)	660, 1386
Sfr274I	CTCGAG	2 cut(s)	683, 951
Sfr303I	CCGCGG	1 cut(s)	70
SgrBI	CCGCGG	1 cut(s)	70
SinI	GGWCC	3 cut(s)	61, 548, 1116
SlaI	CTCGAG	2 cut(s)	683, 951
SmiMI	CAYNNNNRTG	1 cut(s)	1475
SmlI	CTYRAG	2 cut(s)	683, 951
SmoI	CTYRAG	2 cut(s)	683, 951
SphI	GCATGC	1 cut(s)	30
Sse9I	AATT	8 cut(s)	232, 420, 489, 608, 805, 1291, 1515, 1537
SsiI	CCGC	8 cut(s)	67, 69, 83, 128, 194, 720, 1114, 1423
SspMI	CTAG	4 cut(s)	416, 464, 1308, 1524
SstI	GAGCTC	1 cut(s)	684
StyD4I	CCNGG	7 cut(s)	78, 113, 781, 927, 1144, 1365, 1543
StyI	CCWWGG	1 cut(s)	1307
TaaI	ACNGT	5 cut(s)	156, 539, 673, 713, 1234
TaqI	TCGA	5 cut(s)	541, 684, 920, 952, 1061
TaqII	GACCGA	1 cut(s)	815
TasI	AATT	8 cut(s)	232, 420, 489, 608, 805, 1291, 1515, 1537
TatI	WGTACW	4 cut(s)	45, 1034, 1129, 1458
TauI	GCSGC	1 cut(s)	723
TfiI	GAWTC	1 cut(s)	976
Tru1I	TTAA	5 cut(s)	906, 1002, 1100, 1206, 1552
Tru9I	TTAA	5 cut(s)	906, 1002, 1100, 1206, 1552
TscAI	CASTG	5 cut(s)	542, 574, 718, 885, 1447
TseFI	GTSAC	1 cut(s)	388
TseI	GCWGC	4 cut(s)	362, 1339, 1385, 1533
Tsp45I	GTSAC	1 cut(s)	388
TspDTI	ATGAA	4 cut(s)	330, 989, 1201, 1501
TspGWI	ACGGA	4 cut(s)	198, 1270, 1478, 1486
TspRI	CASTG	5 cut(s)	542, 574, 718, 885, 1447
Tth111I	GACNNNGTC	1 cut(s)	1347
VpaK11BI	GGWCC	3 cut(s)	61, 548, 1116
XapI	RAATTY	3 cut(s)	232, 608, 805
XceI	RCATGY	3 cut(s)	30, 746, 768
XhoI	CTCGAG	2 cut(s)	683, 951
XmaJI	CCTAGG	1 cut(s)	1307
XmnI	GAANNNNTTC	1 cut(s)	612
XspI	CTAG	4 cut(s)	416, 464, 1308, 1524
ZrmI	AGTACT	2 cut(s)	47, 1036
Zsp2I	ATGCAT	1 cut(s)	966

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.