Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011494

Physical Location & Seq

Genomic Coordinates Reverse (-)

14540381 .. 14544367

Gene Structure 3987 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1184100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1563 bp

ATGGCCATGGTTGCGAGGCTCCACGTATGCCCACAGAGCTTGGAACACTTCAACTTCAAGATCCACCGCGGAACCGGCCCGGAGTTGATAATCGGGTCGGGTTTTCGGCGGTCCGGGGGGCTGCTGCCCGCGGGGCGAAATGGGTTTTCCTTGAAACCGTGCAGGTCGTTCAGGGGGGAGGATAACGGAGGGGGTGCGGAGGAGGGAGAGGAGGAAAGTGCGAGAAAGCGCGGGAATTTGAAGAAGGGAGGTGGGACTGGGATTTTGAAATCTTTGAAATCTAGTGTTTTGGGGGGTTTTGGTGTGGGGTTTAGAGATGGGGAGGAGTACAGGAACGCGGTAGCCAAGTTGGAGGAGGTCTGTCAATCTGCTGCCGTTCAAATCGGAAGATATATTGTGACCATGATGAGCACAGGAGTTATACTAGCAATTGGGTTTCAGTTGTCAGGTGGAGACAGTGATTTGAATGAGCTAGTTTGGTATAGCTGGCTTGGTGGAATTATTATCGGAACTATGGTTGGATCTAACATGGTTTTAGAGGATCACTGTCGAGCTGGTCCACGAAATGTTGTCATCAGTGGAAGCACAAGGGGACTTGGAAAAGCACTTGCTCGTGAATTTCTTCTTTCTGGAGATCGTGTGGTTGTCGCTTCTCGAAGCCCCGAGTCTGTACAAGCAACTGTCAGAGAGCTTGAGGAAAACCTAAGGGAAGGTATAAACAGTGCAGGCGGGTTATCCAAGAACCTGAAACATGCAAAAGTAGTTGGCGTAGCATGTGATGTTTGTGAAGCCGGCGATGTGCAGAAACTGGCAAATTTTGCTGTTAGTGAACTCGGTCATATTGACATTTGGATAAACAATGCTGGGGCTAATAAAGGATTTAGACCCTTGCTTCAGTTTACAGACGAAGACATTAAGCAGATTGTCTCGACAAACCTGGTTGGGTCTATACTTTGCACTCGAGAAGCAATGCGTATAATGATGAATCAGGCCAAAGGTGGGCATATATTTAATATGGATGGAGCAGGCTCTGGGGGATCAAGTACCCCTTTGACAGCTGTCTATGGATCAACGAAGTGTGGTCTTAGACAGCTTCAATCATCACTTTTAAAGGAGTGCAAGCGGTCCAAAGTAGGAGTGCACACAGCATCTCCAGGCATGGTTCTTACAGATCTGCTTCTGAGTGGCTCTTCTTTGAAGAATAAACAAATGTTTAACTTCATATGCGAGCTTCCTGAAACAGTAGCTAGAACTTTAGTTCCACGGATGCGAGTTGTGAAGGGAACAGGAAAGGCCATCAATTACTTGACACCTCCTAGGATCTTACTTGCTTTAGTAACCGCATGGCTGCGACGTGGTCGCTGGTTTGATGAACAGGGCAAGGCACTGTACGCAGCAGAAGCAGACCGGCTTCGGAACTGGGCAGAAAACCGCACCCGTTTTTACTTCACTGATGCAATGGATATGTACACAGAGAACACTTGGGTGTCTGTCTTCTCACTCTCTGTAGTCTGTGCCTTCATCATCCTCTCTAGCACCAGCAGCAACTTGCCTGGTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006778 GO:0006787 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0010304 GO:0015994 GO:0015996 GO:0016491 GO:0016614 GO:0016616 GO:0019439 GO:0019538 GO:0030163 GO:0033013 GO:0033015 GO:0034256 GO:0034641 GO:0042440 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046149 GO:0046483 GO:0046700 GO:0051186 GO:0051187 GO:0055114 GO:0071704 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 map00860 map01100 map01110

Pfam Domains

Protein Families

adh_short

Protein Analysis

521

Amino Acids

56.11

Weight (kDa)

9.25

Isoelectric Point (pI)

39.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	189 - 392	1.8e-35	short chain dehydrogenase
adh_short_C2	PF13561	196 - 395	1.6e-24	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13250 AT4G13250
fragaria_vesca	FvH4_1g04700
malus_domestica	MD02G1045900.v1.1 MD15G1184100.v1.1
prunus_persica	Prupe.7G232100_v2.0.a1 Prupe.7G232100_v2.0.a1 Prupe.7G232100_v2.0.a1 Prupe.7G232100_v2.0.a1
pyrus_communis	pycom02g03830 pycom15g16570
rosa_chinensis	RchiOBHm_Chr2g0090301
rosa_laevigata	RLG00000016096
rosa_multiflora	Rmu_sc0000436.1_g000005 Rmu_sc0006235.1_g000003
rosa_roxburghii	Rroxscaffold_2G00151300
rosa_rugosa	Rorug02G0006400
rosa_samantha	Rh2BG050900 Rh2CG053100
rosa_wichuraiana	Rw2G004460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	153
AccII	CGCG	4 cut(s)	69, 131, 231, 338
AclWI	GGATC	6 cut(s)	55, 529, 549, 1045, 1075, 1328
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	3 cut(s)	235, 617, 814
AcuI	CTGAAG	1 cut(s)	878
AfaI	GTAC	6 cut(s)	329, 672, 1045, 1391, 1469, 1558
AfiI	CCNNNNNNNGG	1 cut(s)	999
AjiI	CACGTC	1 cut(s)	1355
AjnI	CCWGG	3 cut(s)	936, 1153, 1552
AleI	CACNNNNGTG	1 cut(s)	1484
AloI	GAACNNNNNNTCC	2 cut(s)	1243, 1275
AluBI	AGCT	9 cut(s)	39, 472, 486, 554, 691, 1058, 1093, 1231, 1247
AluI	AGCT	9 cut(s)	39, 472, 486, 554, 691, 1058, 1093, 1231, 1247
Alw21I	GWGCWC	2 cut(s)	413, 1143
Alw26I	GTCTC	2 cut(s)	447, 931
Alw44I	GTGCAC	1 cut(s)	1139
AlwI	GGATC	6 cut(s)	55, 529, 549, 1045, 1075, 1328
AlwNI	CAGNNNCTG	2 cut(s)	808, 1031
Ama87I	CYCGRG	2 cut(s)	662, 960
AoxI	GGCC	4 cut(s)	3, 76, 990, 1293
ApaLI	GTGCAC	1 cut(s)	1139
ApeKI	GCWGC	6 cut(s)	121, 124, 371, 1348, 1394, 1542
ApoI	RAATTY	3 cut(s)	235, 617, 814
ArsI	GACNNNNNNTTYG	2 cut(s)	247, 279
Asp700I	GAANNNNTTC	1 cut(s)	621
AspA2I	CCTAGG	1 cut(s)	1316
AspLEI	GCGC	1 cut(s)	231
AspS9I	GGNCC	4 cut(s)	77, 111, 557, 1125
AsuC2I	CCSGG	2 cut(s)	80, 115
AvaI	CYCGRG	2 cut(s)	662, 960
AvaII	GGWCC	3 cut(s)	111, 557, 1125
AvrII	CCTAGG	1 cut(s)	1316
AxyI	CCTNAGG	1 cut(s)	704
BaeGI	GKGCMC	1 cut(s)	1143
BalI	TGGCCA	1 cut(s)	5
BauI	CACGAG	1 cut(s)	612
BbsI	GAAGAC	2 cut(s)	915, 1486
Bbv12I	GWGCWC	2 cut(s)	413, 1143
BbvI	GCAGC	6 cut(s)	108, 111, 358, 1335, 1406, 1554
BccI	CCATC	3 cut(s)	311, 1013, 1304
BceAI	ACGGC	1 cut(s)	359
BciT130I	CCWGG	3 cut(s)	938, 1155, 1554
BcnI	CCSGG	2 cut(s)	80, 115
BcoDI	GTCTC	2 cut(s)	447, 931
BfaI	CTAG	6 cut(s)	282, 425, 473, 1248, 1317, 1533
BfmI	CTRYAG	1 cut(s)	1506
BfuAI	ACCTGC	1 cut(s)	153
BglI	GCCNNNNNGGC	1 cut(s)	133
BglII	AGATCT	1 cut(s)	1171
BisI	GCNGC	6 cut(s)	122, 125, 372, 1349, 1395, 1543
BlnI	CCTAGG	1 cut(s)	1316
BlsI	GCNGC	6 cut(s)	123, 126, 373, 1350, 1396, 1544
Bme1390I	CCNGG	5 cut(s)	80, 115, 938, 1155, 1554
Bme18I	GGWCC	3 cut(s)	111, 557, 1125
BmeT110I	CYCGRG	2 cut(s)	662, 960
BmgBI	CACGTC	1 cut(s)	1355
BmgT120I	GGNCC	4 cut(s)	77, 111, 557, 1125
BmiI	GGNNCC	2 cut(s)	20, 73
BmrFI	CCNGG	5 cut(s)	80, 115, 938, 1155, 1554
BmrI	ACTGGG	2 cut(s)	267, 1429
BmsI	GCATC	3 cut(s)	1157, 1257, 1444
BmuI	ACTGGG	2 cut(s)	267, 1429
BoxI	GACNNNNGTC	1 cut(s)	1058
BpiI	GAAGAC	2 cut(s)	915, 1486
BpmI	CTGGAG	2 cut(s)	651, 1137
BpuEI	CTTGAG	1 cut(s)	713
BpuMI	CCSGG	2 cut(s)	80, 115
BsaAI	YACGTR	1 cut(s)	25
BsaJI	CCNNGG	6 cut(s)	6, 67, 114, 129, 1262, 1316
BsaXI	ACNNNNNCTCC	4 cut(s)	624, 654, 1135, 1165
Bsc4I	CCNNNNNNNGG	1 cut(s)	999
Bse118I	RCCGGY	3 cut(s)	74, 791, 1407
Bse1I	ACTGG	3 cut(s)	262, 813, 1424
Bse21I	CCTNAGG	1 cut(s)	704
Bse3DI	GCAATG	2 cut(s)	975, 1464
BseBI	CCWGG	3 cut(s)	938, 1155, 1554
BseDI	CCNNGG	6 cut(s)	6, 67, 114, 129, 1262, 1316
BseGI	GGATG	3 cut(s)	1024, 1272, 1524
BseLI	CCNNNNNNNGG	1 cut(s)	999
BseMI	GCAATG	2 cut(s)	975, 1464
BseMII	CTCAG	1 cut(s)	1172
BseNI	ACTGG	3 cut(s)	262, 813, 1424
BseRI	GAGGAG	4 cut(s)	215, 224, 338, 368
BseSI	GKGCMC	1 cut(s)	1143
BseXI	GCAGC	6 cut(s)	108, 111, 358, 1335, 1406, 1554
BseYI	CCCAGC	1 cut(s)	863
BsgI	GTGCAG	3 cut(s)	181, 744, 821
Bsh1236I	CGCG	4 cut(s)	69, 131, 231, 338
BshFI	GGCC	4 cut(s)	5, 78, 992, 1295
BsiHKAI	GWGCWC	2 cut(s)	413, 1143
BsiHKCI	CYCGRG	2 cut(s)	662, 960
BsiSI	CCGG	5 cut(s)	75, 80, 114, 792, 1408
BslFI	GGGAC	2 cut(s)	268, 606
BslI	CCNNNNNNNGG	1 cut(s)	999
BsmAI	GTCTC	2 cut(s)	447, 931
BsmFI	GGGAC	2 cut(s)	268, 606
BsnI	GGCC	4 cut(s)	5, 78, 992, 1295
BsoBI	CYCGRG	2 cut(s)	662, 960
Bsp1286I	GDGCHC	2 cut(s)	413, 1143
Bsp1407I	TGTACA	2 cut(s)	670, 1467
Bsp143I	GATC	8 cut(s)	60, 521, 541, 634, 1037, 1067, 1171, 1320
Bsp19I	CCATGG	1 cut(s)	6
BspANI	GGCC	4 cut(s)	5, 78, 992, 1295
BspCNI	CTCAG	1 cut(s)	1173
BspFNI	CGCG	4 cut(s)	69, 131, 231, 338
BspLI	GGNNCC	2 cut(s)	20, 73
BspMI	ACCTGC	1 cut(s)	153
BspPI	GGATC	6 cut(s)	55, 529, 549, 1045, 1075, 1328
BspQI	GCTCTTC	1 cut(s)	1195
BsrDI	GCAATG	2 cut(s)	975, 1464
BsrFI	RCCGGY	3 cut(s)	74, 791, 1407
BsrGI	TGTACA	2 cut(s)	670, 1467
BsrI	ACTGG	3 cut(s)	262, 813, 1424
BssAI	RCCGGY	3 cut(s)	74, 791, 1407
BssECI	CCNNGG	6 cut(s)	6, 67, 114, 129, 1262, 1316
BssMI	GATC	8 cut(s)	60, 521, 541, 634, 1037, 1067, 1171, 1320
BssSI	CACGAG	1 cut(s)	612
BssT1I	CCWWGG	2 cut(s)	6, 1316
Bst2BI	CACGAG	1 cut(s)	612
Bst2UI	CCWGG	3 cut(s)	938, 1155, 1554
Bst4CI	ACNGT	7 cut(s)	159, 458, 548, 682, 722, 1243, 1389
Bst6I	CTCTTC	1 cut(s)	1195
BstAPI	GCANNNNNTGC	1 cut(s)	818
BstAUI	TGTACA	2 cut(s)	670, 1467
BstBAI	YACGTR	1 cut(s)	25
BstC8I	GCNNGC	7 cut(s)	129, 488, 727, 793, 1027, 1121, 1229
BstDEI	CTNAG	3 cut(s)	704, 1085, 1181
BstDSI	CCRYGG	4 cut(s)	6, 67, 129, 1262
BstF5I	GGATG	3 cut(s)	1024, 1272, 1524
BstFNI	CGCG	4 cut(s)	69, 131, 231, 338
BstHHI	GCGC	1 cut(s)	231
BstKTI	GATC	8 cut(s)	63, 524, 544, 637, 1040, 1070, 1174, 1323
BstMAI	GTCTC	2 cut(s)	447, 931
BstMBI	GATC	8 cut(s)	60, 521, 541, 634, 1037, 1067, 1171, 1320
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 36, 75, 133, 818, 1391, 1400, 1542
BstNI	CCWGG	3 cut(s)	938, 1155, 1554
BstNSI	RCATGY	2 cut(s)	755, 777
BstPAI	GACNNNNGTC	1 cut(s)	1058
BstSCI	CCNGG	5 cut(s)	78, 113, 936, 1153, 1552
BstSFI	CTRYAG	1 cut(s)	1506
BstSLI	GKGCMC	1 cut(s)	1143
BstUI	CGCG	4 cut(s)	69, 131, 231, 338
BstV1I	GCAGC	6 cut(s)	108, 111, 358, 1335, 1406, 1554
BstV2I	GAAGAC	2 cut(s)	915, 1486
BstX2I	RGATCY	4 cut(s)	60, 521, 1171, 1320
BstYI	RGATCY	4 cut(s)	60, 521, 1171, 1320
Bsu36I	CCTNAGG	1 cut(s)	704
BsuRI	GGCC	4 cut(s)	5, 78, 992, 1295
BtgI	CCRYGG	4 cut(s)	6, 67, 129, 1262
BtgZI	GCGATG	1 cut(s)	810
BtrI	CACGTC	1 cut(s)	1355
BtsCI	GGATG	3 cut(s)	1024, 1272, 1524
BtsIMutI	CAGTG	6 cut(s)	463, 544, 583, 727, 1385, 1449
BveI	ACCTGC	1 cut(s)	153
Cac8I	GCNNGC	7 cut(s)	129, 488, 727, 793, 1027, 1121, 1229
CaiI	CAGNNNCTG	2 cut(s)	808, 1031
CfoI	GCGC	1 cut(s)	231
Cfr10I	RCCGGY	3 cut(s)	74, 791, 1407
Cfr13I	GGNCC	4 cut(s)	77, 111, 557, 1125
Cfr42I	CCGCGG	2 cut(s)	70, 132
CpoI	CGGWCCG	1 cut(s)	111
CsiI	ACCWGGT	1 cut(s)	936
Csp6I	GTAC	6 cut(s)	328, 671, 1044, 1390, 1468, 1557
CspCI	CAANNNNNGTGG	2 cut(s)	21, 56
CspI	CGGWCCG	1 cut(s)	111
CviAII	CATG	7 cut(s)	7, 403, 529, 752, 774, 1159, 1344
CviQI	GTAC	6 cut(s)	328, 671, 1044, 1390, 1468, 1557
DdeI	CTNAG	3 cut(s)	704, 1085, 1181
DpnI	GATC	8 cut(s)	62, 523, 543, 636, 1039, 1069, 1173, 1322
DpnII	GATC	8 cut(s)	60, 521, 541, 634, 1037, 1067, 1171, 1320
DraI	TTTAAA	1 cut(s)	1110
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	1 cut(s)	1195
EarI	CTCTTC	1 cut(s)	1195
Eco130I	CCWWGG	2 cut(s)	6, 1316
Eco47I	GGWCC	3 cut(s)	111, 557, 1125
Eco57I	CTGAAG	1 cut(s)	878
Eco81I	CCTNAGG	1 cut(s)	704
Eco88I	CYCGRG	2 cut(s)	662, 960
EcoRII	CCWGG	3 cut(s)	936, 1153, 1552
EcoT14I	CCWWGG	2 cut(s)	6, 1316
ErhI	CCWWGG	2 cut(s)	6, 1316
FaeI	CATG	7 cut(s)	10, 406, 532, 755, 777, 1162, 1347
FalI	AAGNNNNNCTT	2 cut(s)	1033, 1065
FaqI	GGGAC	2 cut(s)	268, 606
FatI	CATG	7 cut(s)	6, 402, 528, 751, 773, 1158, 1343
FauI	CCCGC	4 cut(s)	124, 136, 224, 722
FauNDI	CATATG	1 cut(s)	1223
Fnu4HI	GCNGC	6 cut(s)	122, 125, 372, 1349, 1395, 1543
FokI	GGATG	3 cut(s)	1031, 1279, 1511
Fsp4HI	GCNGC	6 cut(s)	122, 125, 372, 1349, 1395, 1543
FspBI	CTAG	6 cut(s)	282, 425, 473, 1248, 1317, 1533
GlaI	GCGC	1 cut(s)	230
GluI	GCNGC	6 cut(s)	122, 125, 372, 1349, 1395, 1543
GsaI	CCCAGC	1 cut(s)	867
GsuI	CTGGAG	2 cut(s)	651, 1137
HaeIII	GGCC	4 cut(s)	5, 78, 992, 1295
HapII	CCGG	5 cut(s)	75, 80, 114, 792, 1408
HhaI	GCGC	1 cut(s)	231
Hin1II	CATG	7 cut(s)	10, 406, 532, 755, 777, 1162, 1347
Hin6I	GCGC	1 cut(s)	229
HinP1I	GCGC	1 cut(s)	229
HinfI	GANTC	2 cut(s)	665, 985
HpaII	CCGG	5 cut(s)	75, 80, 114, 792, 1408
Hpy166II	GTNNAC	5 cut(s)	560, 830, 900, 1141, 1470
Hpy188I	TCNGA	5 cut(s)	386, 509, 686, 1182, 1416
Hpy188III	TCNNGA	7 cut(s)	58, 614, 630, 654, 928, 962, 1235
Hpy8I	GTNNAC	5 cut(s)	560, 830, 900, 1141, 1470
Hpy99I	CGWCG	1 cut(s)	1356
HpyAV	CCTTC	4 cut(s)	238, 704, 1273, 1528
HpyCH4III	ACNGT	7 cut(s)	159, 458, 548, 682, 722, 1243, 1389
HpyCH4IV	ACGT	2 cut(s)	24, 1354
HpyCH4V	TGCA	8 cut(s)	162, 725, 755, 802, 957, 1119, 1141, 1457
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 36, 75, 133, 818, 1391, 1400, 1542
HpyF3I	CTNAG	3 cut(s)	704, 1085, 1181
HpySE526I	ACGT	2 cut(s)	24, 1354
Hsp92II	CATG	7 cut(s)	10, 406, 532, 755, 777, 1162, 1347
HspAI	GCGC	1 cut(s)	229
KroI	GCCGGC	1 cut(s)	791
KroNI	GCCGGC	1 cut(s)	793
KspI	CCGCGG	2 cut(s)	70, 132
Kzo9I	GATC	8 cut(s)	60, 521, 541, 634, 1037, 1067, 1171, 1320
LguI	GCTCTTC	1 cut(s)	1195
LmnI	GCTCC	2 cut(s)	24, 1022
Lsp1109I	GCAGC	6 cut(s)	108, 111, 358, 1335, 1406, 1554
LweI	GCATC	3 cut(s)	1157, 1257, 1444
MabI	ACCWGGT	1 cut(s)	936
MaeI	CTAG	6 cut(s)	282, 425, 473, 1248, 1317, 1533
MaeII	ACGT	2 cut(s)	24, 1354
MaeIII	GTNAC	2 cut(s)	397, 1336
MalI	GATC	8 cut(s)	62, 523, 543, 636, 1039, 1069, 1173, 1322
MboI	GATC	8 cut(s)	60, 521, 541, 634, 1037, 1067, 1171, 1320
MboII	GAAGA	7 cut(s)	253, 399, 614, 920, 1182, 1210, 1486
MfeI	CAATTG	1 cut(s)	429
MflI	RGATCY	4 cut(s)	60, 521, 1171, 1320
MhlI	GDGCHC	2 cut(s)	413, 1143
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	6 cut(s)	235, 429, 498, 617, 814, 1300
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	674
MmeI	TCCRAC	2 cut(s)	330, 499
Mox20I	TGGCCA	1 cut(s)	5
MroNI	GCCGGC	1 cut(s)	791
MroXI	GAANNNNTTC	1 cut(s)	621
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	4 cut(s)	915, 1011, 1109, 1215
MslI	CAYNNNNRTG	1 cut(s)	1484
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	3 cut(s)	69, 131, 1058
MspI	CCGG	5 cut(s)	75, 80, 114, 792, 1408
MspR9I	CCNGG	5 cut(s)	80, 115, 938, 1155, 1554
MunI	CAATTG	1 cut(s)	429
MvaI	CCWGG	3 cut(s)	938, 1155, 1554
MvnI	CGCG	4 cut(s)	69, 131, 231, 338
MwoI	GCNNNNNNNGC	8 cut(s)	11, 36, 75, 133, 818, 1391, 1400, 1542
NaeI	GCCGGC	1 cut(s)	793
NciI	CCSGG	2 cut(s)	80, 115
NcoI	CCATGG	1 cut(s)	6
NdeI	CATATG	1 cut(s)	1223
NdeII	GATC	8 cut(s)	60, 521, 541, 634, 1037, 1067, 1171, 1320
NgoMIV	GCCGGC	1 cut(s)	791
NlaIII	CATG	7 cut(s)	10, 406, 532, 755, 777, 1162, 1347
NlaIV	GGNNCC	2 cut(s)	20, 73
NmuCI	GTSAC	1 cut(s)	397
NspI	RCATGY	2 cut(s)	755, 777
OliI	CACNNNNGTG	1 cut(s)	1484
PaeR7I	CTCGAG	1 cut(s)	960
PciSI	GCTCTTC	1 cut(s)	1195
PdiI	GCCGGC	1 cut(s)	793
PdmI	GAANNNNTTC	1 cut(s)	621
PfeI	GAWTC	1 cut(s)	985
PflFI	GACNNNGTC	1 cut(s)	1356
PkrI	GCNGC	6 cut(s)	123, 126, 373, 1350, 1396, 1544
PleI	GAGTC	1 cut(s)	673
PpsI	GAGTC	1 cut(s)	673
Ppu21I	YACGTR	1 cut(s)	25
PshAI	GACNNNNGTC	1 cut(s)	1058
Psp6I	CCWGG	3 cut(s)	936, 1153, 1552
PspFI	CCCAGC	1 cut(s)	863
PspGI	CCWGG	3 cut(s)	936, 1153, 1552
PspN4I	GGNNCC	2 cut(s)	20, 73
PspPI	GGNCC	4 cut(s)	77, 111, 557, 1125
PstNI	CAGNNNCTG	2 cut(s)	808, 1031
PsuI	RGATCY	4 cut(s)	60, 521, 1171, 1320
PsyI	GACNNNGTC	1 cut(s)	1356
PvuII	CAGCTG	1 cut(s)	1058
RsaI	GTAC	6 cut(s)	329, 672, 1045, 1391, 1469, 1558
RsaNI	GTAC	6 cut(s)	328, 671, 1044, 1390, 1468, 1557
RseI	CAYNNNNRTG	1 cut(s)	1484
Rsr2I	CGGWCCG	1 cut(s)	111
RsrII	CGGWCCG	1 cut(s)	111
SacII	CCGCGG	2 cut(s)	70, 132
SapI	GCTCTTC	1 cut(s)	1195
SaqAI	TTAA	4 cut(s)	915, 1011, 1109, 1215
SatI	GCNGC	6 cut(s)	122, 125, 372, 1349, 1395, 1543
Sau3AI	GATC	8 cut(s)	60, 521, 541, 634, 1037, 1067, 1171, 1320
Sau96I	GGNCC	4 cut(s)	77, 111, 557, 1125
SchI	GAGTC	1 cut(s)	674
ScrFI	CCNGG	5 cut(s)	80, 115, 938, 1155, 1554
SduI	GDGCHC	2 cut(s)	413, 1143
SexAI	ACCWGGT	1 cut(s)	936
SfaNI	GCATC	3 cut(s)	1157, 1257, 1444
SfcI	CTRYAG	1 cut(s)	1506
Sfr274I	CTCGAG	1 cut(s)	960
Sfr303I	CCGCGG	2 cut(s)	70, 132
SgrBI	CCGCGG	2 cut(s)	70, 132
SinI	GGWCC	3 cut(s)	111, 557, 1125
SlaI	CTCGAG	1 cut(s)	960
SmiMI	CAYNNNNRTG	1 cut(s)	1484
SmlI	CTYRAG	2 cut(s)	692, 960
SmoI	CTYRAG	2 cut(s)	692, 960
Sse9I	AATT	6 cut(s)	235, 429, 498, 617, 814, 1300
SspMI	CTAG	6 cut(s)	282, 425, 473, 1248, 1317, 1533
StyD4I	CCNGG	5 cut(s)	78, 113, 936, 1153, 1552
StyI	CCWWGG	2 cut(s)	6, 1316
TaaI	ACNGT	7 cut(s)	159, 458, 548, 682, 722, 1243, 1389
TaiI	ACGT	2 cut(s)	27, 1357
TaqI	TCGA	4 cut(s)	550, 655, 929, 961
TaqII	GACCGA	1 cut(s)	824
TasI	AATT	6 cut(s)	235, 429, 498, 617, 814, 1300
TatI	WGTACW	3 cut(s)	327, 670, 1467
TfiI	GAWTC	1 cut(s)	985
Tru1I	TTAA	4 cut(s)	915, 1011, 1109, 1215
Tru9I	TTAA	4 cut(s)	915, 1011, 1109, 1215
TscAI	CASTG	6 cut(s)	463, 551, 583, 727, 1392, 1456
TseFI	GTSAC	1 cut(s)	397
TseI	GCWGC	6 cut(s)	121, 124, 371, 1348, 1394, 1542
Tsp45I	GTSAC	1 cut(s)	397
TspDTI	ATGAA	4 cut(s)	998, 1210, 1386, 1510
TspGWI	ACGGA	2 cut(s)	201, 1279
TspRI	CASTG	6 cut(s)	463, 551, 583, 727, 1392, 1456
Tth111I	GACNNNGTC	1 cut(s)	1356
VneI	GTGCAC	1 cut(s)	1139
VpaK11BI	GGWCC	3 cut(s)	111, 557, 1125
XapI	RAATTY	3 cut(s)	235, 617, 814
XceI	RCATGY	2 cut(s)	755, 777
XhoI	CTCGAG	1 cut(s)	960
XmaJI	CCTAGG	1 cut(s)	1316
XmnI	GAANNNNTTC	1 cut(s)	621
XspI	CTAG	6 cut(s)	282, 425, 473, 1248, 1317, 1533

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.