Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011228

Physical Location & Seq

Genomic Coordinates Forward (+)

4774840 .. 4776557

Gene Structure 1718 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1059400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGGATGATTTCTTGAAGGAATTCTTCCCCAACATATACAGAAGGAAGCAACTGCACCTCAATGAGACAGACTACTGTAAATATAATAACCAAATTTTGACACTCTTCACATCCTGTTTGTACTTTGCGGGCCTCATTTCTACATTCGGAGCTTCTTATGTTACCCGAAACAAAGGAAGAAAGGCAAGCATTCTTGTTGGAGCAGTCAGCTTGTTTTTAGGAGCAGTCCTGAATGCTGCTGCAAAAAACATTGCAATGTTGATCATCGGACGAATATTTCTTGGTGTTGGCTTTGGATTTGGAAATCAGGCAGTTCCCTTGTATCTCCGGGAAATGGCTCCTGCGAAAATTCGAGCAGCAGTTAACCAACTTTTCCAGCTGACAGCTTTCTTAGGCATCCTGATCGCTAACTGGATAAATTATGGAACCGATAAAATCCATCCGTGGGGTTGGCGATTGTCTCTTGGATTAGCTACAGTCCCAGCAGTTCTTATGTTTGTTGGGGGTCTTTTTCTTCCTGAGACCCCAAATAGCCTTGTAGAGCAAGGCAGGTTAGAAGAGGCAAGAACTATACTTGAGAAAGTGAGAGGTACCAAAAAAGTTGATGCGGAGTTTGCCAACCTGGTTGATGCTAGCAATGTAGCTCGATCCATAAAGAACCCGTTTAGGAATCTACTCGCGCGAAAAAATCGCCCCCAATTGGTGATAGGGGCCTTGGGAATCCCTGCATTCCAACAGCTCACTGGCATGAGCTCAATCCTCGTCTATGCACCTGTCATATTTCAGAGCTTGGGATTTGGATCGGGGGCAGCTCTGTACTCATCTATCTTCACAAGTGGAGCATTTCTTGTTGCTACATTCATTTCAATTGGATATGTTGATATGTTTGGTAGAAGAGCTTCCTTCTTAGCAGCTGGAACTGAAATGATATGCTGCTTGGTTGCTGTGACCATAACCCTAGCCCTGAAGTTTGGACAAGGAGAAGTCCTCCCAAAAGGGATAGGAATCTTCCTTGTTATCGTCATATGCATATTTGTTTTGGCTTATGGAAGGTCATGGCGTCCTTTGGCGTGGTTAGTTCCAAGTGAGCTGTTTCCTTTAGAGATGAGATCAGCTGGACTGAGTGTCGTTTTCTGTGTCAATCTCCTCTTCACGGCTTTGATAGCGCAGGGCTTCCTCGCGGGGCTTTGCCATCTTCAATATGGTATTTTTCTGCTGTTTGCGGGTTTGATAATCATCATGAATACCTTCATCTTCTTCCTCTTGCCAGAGACAAAGCAAGTCCCCATAGAAGAAATATATCTTCTGTTTCAGAAACATTGGTTTTGGAAAAGAATAGTAGGAGATGGGGAGCAAATTGGACCCAATGGGAAGCCAAGCCAACCAGATGGGAAGTCTGGAGCACAAGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005355 GO:0005402 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015149 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015749 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0034219 GO:0034220 GO:0044464 GO:0046323 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071944 GO:0098655 GO:0098660 GO:0098662 GO:1902600 GO:1904659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

471

Amino Acids

51.65

Weight (kDa)

9.54

Isoelectric Point (pI)

33.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	22 - 435	9.5e-103	Sugar (and other) transporter
MFS_1	PF07690	33 - 384	7.9e-19	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011228)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77210 AT1G77210
fragaria_vesca	FvH4_1g05770 FvH4_1g05770
malus_domestica	MD02G1059400.v1.1 MD15G1193400.v1.1
prunus_persica	Prupe.7G223700_v2.0.a1 Prupe.7G223700_v2.0.a1
pyrus_communis	pycom15g17310
rosa_chinensis	RchiOBHm_Chr2g0091421
rosa_laevigata	RLG00000016222
rosa_multiflora	Rmu_co8499403.1_g000001 Rmu_ssc0000333.1_g000009
rosa_roxburghii	Rroxscaffold_2G00150100
rosa_rugosa	Rorug02G0017700
rosa_samantha	Rh2AG062600 Rh2BG061900 Rh2CG063800 Rh2DG062200
rosa_wichuraiana	Rw2G005750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	540
Acc65I	GGTACC	1 cut(s)	590
AccB1I	GGYRCC	1 cut(s)	590
AccII	CGCG	3 cut(s)	680, 682, 1181
AciI	CCGC	4 cut(s)	128, 608, 1181, 1223
AclWI	GGATC	2 cut(s)	642, 808
AcsI	RAATTY	3 cut(s)	20, 93, 348
AcuI	CTGAAG	1 cut(s)	986
AcyI	GRCGYC	1 cut(s)	1060
AfaI	GTAC	3 cut(s)	122, 592, 818
AfiI	CCNNNNNNNGG	4 cut(s)	334, 445, 700, 1153
AgsI	TTSAA	3 cut(s)	16, 867, 1199
AhdI	GACNNNNNGTC	1 cut(s)	1124
AjnI	CCWGG	1 cut(s)	621
Alw21I	GWGCWC	2 cut(s)	755, 1405
Alw26I	GTCTC	4 cut(s)	59, 465, 515, 1265
AlwI	GGATC	2 cut(s)	642, 808
AoxI	GGCC	2 cut(s)	130, 711
ApeKI	GCWGC	6 cut(s)	236, 239, 356, 809, 911, 933
ApoI	RAATTY	3 cut(s)	20, 93, 348
Asp700I	GAANNNNTTC	3 cut(s)	20, 898, 1247
Asp718I	GGTACC	1 cut(s)	590
AspLEI	GCGC	2 cut(s)	682, 1168
AspS9I	GGNCC	3 cut(s)	130, 711, 1361
AsuC2I	CCSGG	1 cut(s)	329
AsuHPI	GGTGA	1 cut(s)	715
AsuNHI	GCTAGC	1 cut(s)	632
AvaII	GGWCC	1 cut(s)	1361
BanI	GGYRCC	1 cut(s)	590
BanII	GRGCYC	1 cut(s)	755
BarI	GAAGNNNNNNTAC	2 cut(s)	883, 915
Bbv12I	GWGCWC	2 cut(s)	755, 1405
BbvI	GCAGC	6 cut(s)	223, 226, 368, 821, 920, 923
BccI	CCATC	4 cut(s)	447, 1200, 1340, 1382
BceAI	ACGGC	1 cut(s)	1170
BcgI	CGANNNNNNTGC	2 cut(s)	385, 419
BciT130I	CCWGG	1 cut(s)	623
BclI	TGATCA	1 cut(s)	261
BcnI	CCSGG	1 cut(s)	329
BcoDI	GTCTC	4 cut(s)	59, 465, 515, 1265
BfaI	CTAG	2 cut(s)	633, 959
BfmI	CTRYAG	1 cut(s)	474
BfuAI	ACCTGC	1 cut(s)	540
BisI	GCNGC	6 cut(s)	237, 240, 357, 810, 912, 934
BlsI	GCNGC	6 cut(s)	238, 241, 358, 811, 913, 935
Bme1390I	CCNGG	2 cut(s)	329, 623
Bme18I	GGWCC	1 cut(s)	1361
BmeRI	GACNNNNNGTC	1 cut(s)	1124
BmgT120I	GGNCC	3 cut(s)	130, 711, 1361
BmiI	GGNNCC	5 cut(s)	339, 427, 592, 712, 1363
BmrFI	CCNGG	2 cut(s)	329, 623
BmsI	GCATC	3 cut(s)	405, 595, 619
BmtI	GCTAGC	1 cut(s)	636
BplI	GAGNNNNNCTC	2 cut(s)	972, 1004
BpuEI	CTTGAG	1 cut(s)	596
BpuMI	CCSGG	1 cut(s)	329
BsaBI	GATNNNNATC	3 cut(s)	799, 1004, 1235
BsaHI	GRCGYC	1 cut(s)	1060
BsaI	GGTCTC	1 cut(s)	515
BsaJI	CCNNGG	2 cut(s)	443, 714
BsaXI	ACNNNNNCTCC	1 cut(s)	1392
Bsc4I	CCNNNNNNNGG	4 cut(s)	334, 445, 700, 1153
Bse1I	ACTGG	2 cut(s)	416, 748
Bse3DI	GCAATG	3 cut(s)	249, 261, 643
Bse8I	GATNNNNATC	3 cut(s)	799, 1004, 1235
BseBI	CCWGG	1 cut(s)	623
BseDI	CCNNGG	2 cut(s)	443, 714
BseGI	GGATG	4 cut(s)	10, 110, 396, 439
BseJI	GATNNNNATC	3 cut(s)	799, 1004, 1235
BseLI	CCNNNNNNNGG	4 cut(s)	334, 445, 700, 1153
BseMI	GCAATG	3 cut(s)	249, 261, 643
BseMII	CTCAG	2 cut(s)	510, 1112
BseNI	ACTGG	2 cut(s)	416, 748
BseRI	GAGGAG	1 cut(s)	1136
BseXI	GCAGC	6 cut(s)	223, 226, 368, 821, 920, 923
BseYI	CCCAGC	1 cut(s)	481
BsgI	GTGCAG	1 cut(s)	38
Bsh1236I	CGCG	3 cut(s)	680, 682, 1181
BshFI	GGCC	2 cut(s)	132, 713
BshNI	GGYRCC	1 cut(s)	590
BsiHKAI	GWGCWC	2 cut(s)	755, 1405
BsiSI	CCGG	1 cut(s)	328
BslFI	GGGAC	2 cut(s)	464, 1268
BslI	CCNNNNNNNGG	4 cut(s)	334, 445, 700, 1153
BsmAI	GTCTC	4 cut(s)	59, 465, 515, 1265
BsmFI	GGGAC	2 cut(s)	464, 1268
BsmI	GAATGC	3 cut(s)	189, 238, 728
BsnI	GGCC	2 cut(s)	132, 713
Bso31I	GGTCTC	1 cut(s)	515
Bsp1286I	GDGCHC	2 cut(s)	755, 1405
Bsp143I	GATC	5 cut(s)	261, 402, 647, 800, 1109
BspACI	CCGC	4 cut(s)	128, 608, 1181, 1223
BspANI	GGCC	2 cut(s)	132, 713
BspCNI	CTCAG	2 cut(s)	511, 1113
BspFNI	CGCG	3 cut(s)	680, 682, 1181
BspHI	TCATGA	1 cut(s)	1239
BspLI	GGNNCC	5 cut(s)	339, 427, 592, 712, 1363
BspMI	ACCTGC	1 cut(s)	540
BspOI	GCTAGC	1 cut(s)	636
BspPI	GGATC	2 cut(s)	642, 808
BspQI	GCTCTTC	1 cut(s)	889
BspT107I	GGYRCC	1 cut(s)	590
BspTNI	GGTCTC	1 cut(s)	515
BsrDI	GCAATG	3 cut(s)	249, 261, 643
BsrI	ACTGG	2 cut(s)	416, 748
BssECI	CCNNGG	2 cut(s)	443, 714
BssMI	GATC	5 cut(s)	261, 402, 647, 800, 1109
BssNI	GRCGYC	1 cut(s)	1060
BssT1I	CCWWGG	1 cut(s)	714
Bst2UI	CCWGG	1 cut(s)	623
Bst4CI	ACNGT	2 cut(s)	77, 478
Bst6I	CTCTTC	4 cut(s)	110, 552, 889, 1154
BstACI	GRCGYC	1 cut(s)	1060
BstC8I	GCNNGC	3 cut(s)	130, 187, 634
BstDEI	CTNAG	4 cut(s)	391, 519, 907, 1121
BstDSI	CCRYGG	1 cut(s)	443
BstF5I	GGATG	4 cut(s)	10, 110, 396, 439
BstFNI	CGCG	3 cut(s)	680, 682, 1181
BstHHI	GCGC	2 cut(s)	682, 1168
BstKTI	GATC	5 cut(s)	264, 405, 650, 803, 1112
BstMAI	GTCTC	4 cut(s)	59, 465, 515, 1265
BstMBI	GATC	5 cut(s)	261, 402, 647, 800, 1109
BstMWI	GCNNNNNNNGC	2 cut(s)	614, 1163
BstNI	CCWGG	1 cut(s)	623
BstSCI	CCNGG	2 cut(s)	327, 621
BstSFI	CTRYAG	1 cut(s)	474
BstUI	CGCG	3 cut(s)	680, 682, 1181
BstV1I	GCAGC	6 cut(s)	223, 226, 368, 821, 920, 923
BstXI	CCANNNNNNTGG	1 cut(s)	1388
BsuRI	GGCC	2 cut(s)	132, 713
BtgI	CCRYGG	1 cut(s)	443
BtsCI	GGATG	4 cut(s)	10, 110, 396, 439
BtsIMutI	CAGTG	1 cut(s)	741
BveI	ACCTGC	1 cut(s)	540
Cac8I	GCNNGC	3 cut(s)	130, 187, 634
CciI	TCATGA	1 cut(s)	1239
CfoI	GCGC	2 cut(s)	682, 1168
Cfr13I	GGNCC	3 cut(s)	130, 711, 1361
CseI	GACGC	1 cut(s)	1049
CsiI	ACCWGGT	1 cut(s)	621
Csp6I	GTAC	3 cut(s)	121, 591, 817
CviAII	CATG	3 cut(s)	748, 1056, 1240
CviQI	GTAC	3 cut(s)	121, 591, 817
DdeI	CTNAG	4 cut(s)	391, 519, 907, 1121
DpnI	GATC	5 cut(s)	263, 404, 649, 802, 1111
DpnII	GATC	5 cut(s)	261, 402, 647, 800, 1109
DriI	GACNNNNNGTC	1 cut(s)	1124
Eam1104I	CTCTTC	4 cut(s)	110, 552, 889, 1154
Eam1105I	GACNNNNNGTC	1 cut(s)	1124
EarI	CTCTTC	4 cut(s)	110, 552, 889, 1154
Ecl136II	GAGCTC	1 cut(s)	753
Eco130I	CCWWGG	1 cut(s)	714
Eco24I	GRGCYC	1 cut(s)	755
Eco31I	GGTCTC	1 cut(s)	515
Eco47I	GGWCC	1 cut(s)	1361
Eco53kI	GAGCTC	1 cut(s)	753
Eco57I	CTGAAG	1 cut(s)	986
EcoICRI	GAGCTC	1 cut(s)	753
EcoO109I	RGGNCCY	1 cut(s)	711
EcoRI	GAATTC	1 cut(s)	20
EcoRII	CCWGG	1 cut(s)	621
EcoT14I	CCWWGG	1 cut(s)	714
EcoT22I	ATGCAT	1 cut(s)	1031
EcoT38I	GRGCYC	1 cut(s)	755
ErhI	CCWWGG	1 cut(s)	714
FaeI	CATG	3 cut(s)	751, 1059, 1243
FalI	AAGNNNNNCTT	2 cut(s)	8, 40
FaqI	GGGAC	2 cut(s)	464, 1268
FatI	CATG	3 cut(s)	747, 1055, 1239
FauI	CCCGC	3 cut(s)	121, 1174, 1216
FauNDI	CATATG	1 cut(s)	1025
FbaI	TGATCA	1 cut(s)	261
Fnu4HI	GCNGC	6 cut(s)	237, 240, 357, 810, 912, 934
FokI	GGATG	4 cut(s)	17, 97, 383, 426
FriOI	GRGCYC	1 cut(s)	755
Fsp4HI	GCNGC	6 cut(s)	237, 240, 357, 810, 912, 934
FspBI	CTAG	2 cut(s)	633, 959
GlaI	GCGC	2 cut(s)	681, 1167
GluI	GCNGC	6 cut(s)	237, 240, 357, 810, 912, 934
GsaI	CCCAGC	1 cut(s)	485
HaeIII	GGCC	2 cut(s)	132, 713
HapII	CCGG	1 cut(s)	328
HgaI	GACGC	1 cut(s)	1049
HhaI	GCGC	2 cut(s)	682, 1168
Hin1I	GRCGYC	1 cut(s)	1060
Hin1II	CATG	3 cut(s)	751, 1059, 1243
Hin6I	GCGC	2 cut(s)	680, 1166
HinP1I	GCGC	2 cut(s)	680, 1166
HincII	GTYRAC	1 cut(s)	364
HindII	GTYRAC	1 cut(s)	364
HinfI	GANTC	3 cut(s)	670, 720, 1005
HpaI	GTTAAC	1 cut(s)	364
HpaII	CCGG	1 cut(s)	328
HphI	GGTGA	1 cut(s)	715
Hpy166II	GTNNAC	1 cut(s)	364
Hpy188I	TCNGA	4 cut(s)	149, 269, 786, 1314
Hpy188III	TCNNGA	6 cut(s)	13, 229, 400, 518, 1240, 1398
Hpy8I	GTNNAC	1 cut(s)	364
HpyAV	CCTTC	5 cut(s)	10, 36, 913, 1044, 1258
HpyCH4III	ACNGT	2 cut(s)	77, 478
HpyCH4V	TGCA	6 cut(s)	55, 242, 254, 728, 770, 1029
HpyF10VI	GCNNNNNNNGC	2 cut(s)	614, 1163
HpyF3I	CTNAG	4 cut(s)	391, 519, 907, 1121
Hsp92I	GRCGYC	1 cut(s)	1060
Hsp92II	CATG	3 cut(s)	751, 1059, 1243
HspAI	GCGC	2 cut(s)	680, 1166
KpnI	GGTACC	1 cut(s)	594
Ksp22I	TGATCA	1 cut(s)	261
KspAI	GTTAAC	1 cut(s)	364
Kzo9I	GATC	5 cut(s)	261, 402, 647, 800, 1109
LguI	GCTCTTC	1 cut(s)	889
LmnI	GCTCC	7 cut(s)	149, 200, 221, 343, 839, 1351, 1400
Lsp1109I	GCAGC	6 cut(s)	223, 226, 368, 821, 920, 923
LweI	GCATC	3 cut(s)	405, 595, 619
MabI	ACCWGGT	1 cut(s)	621
MaeI	CTAG	2 cut(s)	633, 959
MaeIII	GTNAC	2 cut(s)	160, 946
MalI	GATC	5 cut(s)	263, 404, 649, 802, 1111
MboI	GATC	5 cut(s)	261, 402, 647, 800, 1109
MfeI	CAATTG	2 cut(s)	698, 867
MhlI	GDGCHC	2 cut(s)	755, 1405
MluCI	AATT	7 cut(s)	20, 93, 348, 418, 698, 867, 1356
MmeI	TCCRAC	2 cut(s)	178, 757
MnlI	CCTC	9 cut(s)	68, 143, 553, 581, 770, 998, 1157, 1187, 1271
Mph1103I	ATGCAT	1 cut(s)	1031
MroXI	GAANNNNTTC	3 cut(s)	20, 898, 1247
MseI	TTAA	1 cut(s)	363
MslI	CAYNNNNRTG	3 cut(s)	60, 254, 746
MspA1I	CMGCKG	3 cut(s)	379, 914, 1115
MspI	CCGG	1 cut(s)	328
MspR9I	CCNGG	2 cut(s)	329, 623
MunI	CAATTG	2 cut(s)	698, 867
Mva1269I	GAATGC	3 cut(s)	189, 238, 728
MvaI	CCWGG	1 cut(s)	623
MvnI	CGCG	3 cut(s)	680, 682, 1181
MwoI	GCNNNNNNNGC	2 cut(s)	614, 1163
NciI	CCSGG	1 cut(s)	329
NdeI	CATATG	1 cut(s)	1025
NdeII	GATC	5 cut(s)	261, 402, 647, 800, 1109
NheI	GCTAGC	1 cut(s)	632
NlaIII	CATG	3 cut(s)	751, 1059, 1243
NlaIV	GGNNCC	5 cut(s)	339, 427, 592, 712, 1363
NmuCI	GTSAC	1 cut(s)	946
NsiI	ATGCAT	1 cut(s)	1031
PagI	TCATGA	1 cut(s)	1239
PciSI	GCTCTTC	1 cut(s)	889
PctI	GAATGC	3 cut(s)	189, 238, 728
PdmI	GAANNNNTTC	3 cut(s)	20, 898, 1247
PfeI	GAWTC	3 cut(s)	670, 720, 1005
PfoI	TCCNGGA	1 cut(s)	327
PkrI	GCNGC	6 cut(s)	238, 241, 358, 811, 913, 935
Psp124BI	GAGCTC	1 cut(s)	755
Psp6I	CCWGG	1 cut(s)	621
PspFI	CCCAGC	1 cut(s)	481
PspGI	CCWGG	1 cut(s)	621
PspN4I	GGNNCC	5 cut(s)	339, 427, 592, 712, 1363
PspPI	GGNCC	3 cut(s)	130, 711, 1361
PvuII	CAGCTG	3 cut(s)	379, 914, 1115
RsaI	GTAC	3 cut(s)	122, 592, 818
RsaNI	GTAC	3 cut(s)	121, 591, 817
RseI	CAYNNNNRTG	3 cut(s)	60, 254, 746
SacI	GAGCTC	1 cut(s)	755
SapI	GCTCTTC	1 cut(s)	889
SaqAI	TTAA	1 cut(s)	363
SatI	GCNGC	6 cut(s)	237, 240, 357, 810, 912, 934
Sau3AI	GATC	5 cut(s)	261, 402, 647, 800, 1109
Sau96I	GGNCC	3 cut(s)	130, 711, 1361
ScrFI	CCNGG	2 cut(s)	329, 623
SduI	GDGCHC	2 cut(s)	755, 1405
SexAI	ACCWGGT	1 cut(s)	621
SfaNI	GCATC	3 cut(s)	405, 595, 619
SfcI	CTRYAG	1 cut(s)	474
SinI	GGWCC	1 cut(s)	1361
SmiMI	CAYNNNNRTG	3 cut(s)	60, 254, 746
SmlI	CTYRAG	1 cut(s)	575
SmoI	CTYRAG	1 cut(s)	575
Sse9I	AATT	7 cut(s)	20, 93, 348, 418, 698, 867, 1356
SsiI	CCGC	4 cut(s)	128, 608, 1181, 1223
SspI	AATATT	1 cut(s)	276
SspMI	CTAG	2 cut(s)	633, 959
SstI	GAGCTC	1 cut(s)	755
StyD4I	CCNGG	2 cut(s)	327, 621
StyI	CCWWGG	1 cut(s)	714
TaaI	ACNGT	2 cut(s)	77, 478
TaqI	TCGA	2 cut(s)	352, 646
TasI	AATT	7 cut(s)	20, 93, 348, 418, 698, 867, 1356
TatI	WGTACW	2 cut(s)	120, 816
TfiI	GAWTC	3 cut(s)	670, 720, 1005
Tru1I	TTAA	1 cut(s)	363
Tru9I	TTAA	1 cut(s)	363
TscAI	CASTG	1 cut(s)	748
TseFI	GTSAC	1 cut(s)	946
TseI	GCWGC	6 cut(s)	236, 239, 356, 809, 911, 933
Tsp45I	GTSAC	1 cut(s)	946
TspDTI	ATGAA	3 cut(s)	850, 1240, 1256
TspGWI	ACGGA	1 cut(s)	432
TspRI	CASTG	1 cut(s)	748
VpaK11BI	GGWCC	1 cut(s)	1361
XapI	RAATTY	3 cut(s)	20, 93, 348
XcmI	CCANNNNNNNNNTGG	2 cut(s)	740, 1199
XmnI	GAANNNNTTC	3 cut(s)	20, 898, 1247
XspI	CTAG	2 cut(s)	633, 959
Zsp2I	ATGCAT	1 cut(s)	1031

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.