MD02G1064500.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0021154
Physical Location & Seq
Forward (+)
5210282 .. 5211954
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1064500.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGTCAATGGAGCCTTTCACTTTCCCTTTGCGCAACAACTCTCCTTCACCTCCTGTCGTCGAGCAATCAAAGGTTGTTTTGATAGAGGCTGAGAATGTTGAAGTGCCCGTAGAGGTAGCAAGAATTCCCGAACAGGGTTCAGCAGCTCCATCTTTACCAGCCACACATGAAGAGGCCTATCTAACTATCAACCTTCCCCATACCCCATCAATTGGAGTAGTGGATATTGACGGTTCGTCAGGGGAGAAAGAGAAGGACCCTAGCTCGACAAAAGAAGCTCACAACACATATTTCCTTGTTCCTCATTCTGTGCCTGATGCTAGCAAGGAAAAGCCTAGCACACCCTCTCAGAGCAACAAGCAAAAAGAAAAGGCTTCTTCCTCATCATCCAACTTACCAGAACAGCCAACTCCTCCAGCTGTCGTTTATCCCAACATATCCCTCCCTGAGGTCTTGCTCGAACTTCAGAAACTCAAGCAAAAGGTTGACTCCCCGAGCCAGAAGCCATCAGTCCAACAAGCTCAAGATCTAAAACAATCCTTCAAACACTGGTTGGAAGGTGGTTTTGATGTCAACTTCCAACGCGACATGCTTAATCAAGCTGAGAGTACACTTGAGAAGCTGTATGAGCTTAATGAGATCACCGAAGAGCAATTCAAGACCTTTAAATCCTTCTTCCAGTTTCTAAAGCAGTTGCAGGAACAATACTTCCAAGCTGCCAAAGAGGCTGAAGATATGCAACAAATGAAACTCCAACACTCTGAGTTCTCTACTCTTTTGAAATCTTTTATTGGAGAAGGAACCGAAATTAGGGATAGTATTTCTGTAGTGGACCAACAAATTCAGCAGCTAGAGAAAGAGTTGGCCAAACTGAAAGCTGAGAAAGCTAGCTTGGCTAGTGAACTAGCTGTGAAAGTGGTTGAGGCCCGAAGTGCAAGTCAACAAGTAGATATTTTGGATGCTCAGTTGGTCAACAGCAACATCGCAGTTGAAGAACCTGAGAGGCTGGAGATTGACATGAAGACTGTGTATGCTAGGATTGTTGCCCTAGCTAAGGATGCTCAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

357

Amino Acids

39.69

Weight (kDa)

4.88

Isoelectric Point (pI)

60.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021154)

Species Orthologous Gene IDs
malus_domestica MD02G1064500.v1.1 MD02G1064700.v1.1 MD15G1195200.v1.1
prunus_persica Prupe.7G221300_v2.0.a1
pyrus_communis pycom02g05080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 32
AccB7I CCANNNNNTGG 1 cut(s) 212
AccII CGCG 1 cut(s) 585
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 2 cut(s) 123, 840
AcuI CTGAAG 2 cut(s) 449, 750
AfaI GTAC 1 cut(s) 610
AfiI CCNNNNNNNGG 5 cut(s) 134, 212, 448, 810, 1054
AgsI TTSAA 5 cut(s) 101, 544, 658, 781, 992
AhdI GACNNNNNGTC 1 cut(s) 235
AjuI GAANNNNNNNTTGG 2 cut(s) 875, 907
AloI GAACNNNNNNTCC 2 cut(s) 693, 725
Ama87I CYCGRG 1 cut(s) 493
AoxI GGCC 3 cut(s) 174, 864, 924
ApeKI GCWGC 3 cut(s) 143, 716, 847
ApoI RAATTY 2 cut(s) 123, 840
ArsI GACNNNNNNTTYG 2 cut(s) 922, 954
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 3 cut(s) 256, 832, 925
AsuHPI GGTGA 2 cut(s) 39, 634
AsuNHI GCTAGC 2 cut(s) 320, 887
AvaI CYCGRG 1 cut(s) 493
AvaII GGWCC 2 cut(s) 256, 832
AxyI CCTNAGG 1 cut(s) 447
BaeGI GKGCMC 1 cut(s) 108
BalI TGGCCA 1 cut(s) 866
BbsI GAAGAC 1 cut(s) 1028
BbvI GCAGC 3 cut(s) 155, 703, 859
BccI CCATC 3 cut(s) 157, 214, 514
BfaI CTAG 9 cut(s) 261, 321, 336, 851, 888, 897, 905, 1035, 1049
BfmI CTRYAG 1 cut(s) 825
BglI GCCNNNNNGGC 1 cut(s) 725
BglII AGATCT 1 cut(s) 526
BisI GCNGC 3 cut(s) 144, 717, 848
BlsI GCNGC 3 cut(s) 145, 718, 849
Bme18I GGWCC 2 cut(s) 256, 832
BmeRI GACNNNNNGTC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 493
BmgT120I GGNCC 3 cut(s) 256, 832, 925
BmiI GGNNCC 3 cut(s) 12, 258, 802
BmsI GCATC 3 cut(s) 307, 949, 1048
BmtI GCTAGC 2 cut(s) 324, 891
BpiI GAAGAC 1 cut(s) 1028
BpmI CTGGAG 2 cut(s) 399, 1028
Bpu10I CCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 3 cut(s) 458, 507, 635
Bsc4I CCNNNNNNNGG 5 cut(s) 134, 212, 448, 810, 1054
Bse1I ACTGG 2 cut(s) 554, 679
Bse21I CCTNAGG 1 cut(s) 447
BseGI GGATG 3 cut(s) 386, 964, 1063
BseLI CCNNNNNNNGG 5 cut(s) 134, 212, 448, 810, 1054
BseMII CTCAG 8 cut(s) 81, 362, 438, 594, 753, 870, 977, 990
BseNI ACTGG 2 cut(s) 554, 679
BseRI GAGGAG 1 cut(s) 402
BseSI GKGCMC 1 cut(s) 108
BseXI GCAGC 3 cut(s) 155, 703, 859
Bsh1236I CGCG 1 cut(s) 585
BshFI GGCC 3 cut(s) 176, 866, 926
BsiHKCI CYCGRG 1 cut(s) 493
BslI CCNNNNNNNGG 5 cut(s) 134, 212, 448, 810, 1054
BsnI GGCC 3 cut(s) 176, 866, 926
BsoBI CYCGRG 1 cut(s) 493
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 2 cut(s) 526, 639
BspANI GGCC 3 cut(s) 176, 866, 926
BspCNI CTCAG 8 cut(s) 82, 361, 439, 595, 754, 871, 976, 991
BspFNI CGCG 1 cut(s) 585
BspLI GGNNCC 3 cut(s) 12, 258, 802
BspOI GCTAGC 2 cut(s) 324, 891
BspQI GCTCTTC 1 cut(s) 642
BsrI ACTGG 2 cut(s) 554, 679
BssMI GATC 2 cut(s) 526, 639
Bst4CI ACNGT 2 cut(s) 233, 1027
Bst6I CTCTTC 2 cut(s) 165, 642
BstC8I GCNNGC 2 cut(s) 322, 889
BstDEI CTNAG 9 cut(s) 90, 348, 447, 603, 762, 879, 963, 999, 1053
BstENI CCTNNNNNAGG 2 cut(s) 446, 1052
BstF5I GGATG 3 cut(s) 386, 964, 1063
BstFNI CGCG 1 cut(s) 585
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 2 cut(s) 529, 642
BstMBI GATC 2 cut(s) 526, 639
BstMWI GCNNNNNNNGC 6 cut(s) 628, 725, 884, 893, 932, 1058
BstNSI RCATGY 1 cut(s) 592
BstSFI CTRYAG 1 cut(s) 825
BstSLI GKGCMC 1 cut(s) 108
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 3 cut(s) 155, 703, 859
BstV2I GAAGAC 1 cut(s) 1028
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
Bsu36I CCTNAGG 1 cut(s) 447
BsuRI GGCC 3 cut(s) 176, 866, 926
BtgZI GCGATG 1 cut(s) 967
BtsCI GGATG 3 cut(s) 386, 964, 1063
BtsIMutI CAGTG 1 cut(s) 547
Cac8I GCNNGC 2 cut(s) 322, 889
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 3 cut(s) 256, 832, 925
Csp6I GTAC 1 cut(s) 609
CviAII CATG 3 cut(s) 167, 589, 1018
CviQI GTAC 1 cut(s) 609
DdeI CTNAG 9 cut(s) 90, 348, 447, 603, 762, 879, 963, 999, 1053
DpnI GATC 2 cut(s) 528, 641
DpnII GATC 2 cut(s) 526, 639
DraI TTTAAA 1 cut(s) 667
DriI GACNNNNNGTC 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 864
Eam1104I CTCTTC 2 cut(s) 165, 642
Eam1105I GACNNNNNGTC 1 cut(s) 235
EarI CTCTTC 2 cut(s) 165, 642
Eco147I AGGCCT 1 cut(s) 176
Eco47I GGWCC 2 cut(s) 256, 832
Eco57I CTGAAG 2 cut(s) 449, 750
Eco81I CCTNAGG 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 493
EcoNI CCTNNNNNAGG 2 cut(s) 446, 1052
EcoO109I RGGNCCY 1 cut(s) 256
EcoRI GAATTC 1 cut(s) 123
FaeI CATG 3 cut(s) 170, 592, 1021
FaiI YATR 9 cut(s) 168, 201, 289, 437, 590, 627, 737, 1019, 1032
FatI CATG 3 cut(s) 166, 588, 1017
Fnu4HI GCNGC 3 cut(s) 144, 717, 848
FokI GGATG 2 cut(s) 373, 971
Fsp4HI GCNGC 3 cut(s) 144, 717, 848
FspBI CTAG 9 cut(s) 261, 321, 336, 851, 888, 897, 905, 1035, 1049
FspI TGCGCA 1 cut(s) 32
GlaI GCGC 1 cut(s) 32
GluI GCNGC 3 cut(s) 144, 717, 848
GsuI CTGGAG 2 cut(s) 399, 1028
HaeIII GGCC 3 cut(s) 176, 866, 926
HhaI GCGC 1 cut(s) 33
Hin1II CATG 3 cut(s) 170, 592, 1021
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 4 cut(s) 487, 574, 941, 973
HindII GTYRAC 4 cut(s) 487, 574, 941, 973
HinfI GANTC 1 cut(s) 488
HphI GGTGA 2 cut(s) 39, 634
Hpy166II GTNNAC 7 cut(s) 487, 574, 611, 832, 902, 941, 973
Hpy188I TCNGA 3 cut(s) 351, 468, 763
Hpy188III TCNNGA 3 cut(s) 128, 524, 658
Hpy8I GTNNAC 7 cut(s) 487, 574, 611, 832, 902, 941, 973
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 7 cut(s) 54, 203, 247, 550, 551, 682, 791
HpyCH4III ACNGT 2 cut(s) 233, 1027
HpyCH4V TGCA 3 cut(s) 697, 739, 935
HpyF10VI GCNNNNNNNGC 6 cut(s) 628, 725, 884, 893, 932, 1058
HpyF3I CTNAG 9 cut(s) 90, 348, 447, 603, 762, 879, 963, 999, 1053
Hsp92II CATG 3 cut(s) 170, 592, 1021
HspAI GCGC 1 cut(s) 31
Kzo9I GATC 2 cut(s) 526, 639
LguI GCTCTTC 1 cut(s) 642
LmnI GCTCC 2 cut(s) 10, 151
Lsp1109I GCAGC 3 cut(s) 155, 703, 859
LweI GCATC 3 cut(s) 307, 949, 1048
MaeI CTAG 9 cut(s) 261, 321, 336, 851, 888, 897, 905, 1035, 1049
MalI GATC 2 cut(s) 528, 641
MboI GATC 2 cut(s) 526, 639
MboII GAAGA 7 cut(s) 182, 369, 659, 667, 743, 1004, 1033
MfeI CAATTG 2 cut(s) 210, 1064
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 1 cut(s) 108
MlsI TGGCCA 1 cut(s) 866
MluCI AATT 6 cut(s) 123, 210, 653, 807, 840, 1064
MluNI TGGCCA 1 cut(s) 866
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 5 cut(s) 414, 534, 538, 604, 778
Mox20I TGGCCA 1 cut(s) 866
MscI TGGCCA 1 cut(s) 866
MseI TTAA 3 cut(s) 594, 633, 666
Msp20I TGGCCA 1 cut(s) 866
MspA1I CMGCKG 1 cut(s) 419
MunI CAATTG 2 cut(s) 210, 1064
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 6 cut(s) 628, 725, 884, 893, 932, 1058
NdeII GATC 2 cut(s) 526, 639
NheI GCTAGC 2 cut(s) 320, 887
NlaIII CATG 3 cut(s) 170, 592, 1021
NlaIV GGNNCC 3 cut(s) 12, 258, 802
NsbI TGCGCA 1 cut(s) 32
NspI RCATGY 1 cut(s) 592
PceI AGGCCT 1 cut(s) 176
PciSI GCTCTTC 1 cut(s) 642
PflMI CCANNNNNTGG 1 cut(s) 212
PkrI GCNGC 3 cut(s) 145, 718, 849
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
PpuMI RGGWCCY 1 cut(s) 256
Psp5II RGGWCCY 1 cut(s) 256
PspN4I GGNNCC 3 cut(s) 12, 258, 802
PspPI GGNCC 3 cut(s) 256, 832, 925
PspPPI RGGWCCY 1 cut(s) 256
PsuI RGATCY 1 cut(s) 526
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 610
RsaNI GTAC 1 cut(s) 609
SapI GCTCTTC 1 cut(s) 642
SaqAI TTAA 3 cut(s) 594, 633, 666
SatI GCNGC 3 cut(s) 144, 717, 848
Sau3AI GATC 2 cut(s) 526, 639
Sau96I GGNCC 3 cut(s) 256, 832, 925
SchI GAGTC 1 cut(s) 482
SduI GDGCHC 1 cut(s) 108
SfaNI GCATC 3 cut(s) 307, 949, 1048
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 2 cut(s) 256, 832
SmlI CTYRAG 3 cut(s) 473, 522, 614
SmoI CTYRAG 3 cut(s) 473, 522, 614
Sse9I AATT 6 cut(s) 123, 210, 653, 807, 840, 1064
SseBI AGGCCT 1 cut(s) 176
SspMI CTAG 9 cut(s) 261, 321, 336, 851, 888, 897, 905, 1035, 1049
StuI AGGCCT 1 cut(s) 176
TaaI ACNGT 2 cut(s) 233, 1027
TaqI TCGA 3 cut(s) 60, 266, 459
TasI AATT 6 cut(s) 123, 210, 653, 807, 840, 1064
TatI WGTACW 1 cut(s) 608
Tru1I TTAA 3 cut(s) 594, 633, 666
Tru9I TTAA 3 cut(s) 594, 633, 666
TscAI CASTG 1 cut(s) 554
TseI GCWGC 3 cut(s) 143, 716, 847
TspDTI ATGAA 3 cut(s) 183, 761, 1034
TspRI CASTG 1 cut(s) 554
Van91I CCANNNNNTGG 1 cut(s) 212
VpaK11BI GGWCC 2 cut(s) 256, 832
XagI CCTNNNNNAGG 2 cut(s) 446, 1052
XapI RAATTY 2 cut(s) 123, 840
XceI RCATGY 1 cut(s) 592
XspI CTAG 9 cut(s) 261, 321, 336, 851, 888, 897, 905, 1035, 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.