MD15G1195200.v1.1
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0021154
Physical Location & Seq
Forward (+)
15457389 .. 15458926
1538 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1195200.v1.1.491

Sequence Viewer

Length: 858 bp
ATGGATCACCACCATCATCAGAACAACTGCAAAGTCAGTAACACAGAGTTCGCGGATGTTGATTTCGATGACCCCATCTTCTCAACTCCCATTCTTTCATCTCTCAACTTACCCCAACGCACCACCACCGCCAGCGCCACAGTTGCGCTGCCCGACGCCGACTGCCCACAACCGCAACTGCACGTGCCGCTGCCGCCGAAGGTGCAGCAAAAACTGAACACCATATGCGAGGAAAAAGAGCAGCCGCAGCCTGACGCCGAGTTGAGAAGGAAGCTGGGTTTGCTGGGCGAGGAGAAGGCACTCGAACTCCTCGGTGAAATTTACAACACGAAAACAAAGATCAGAACCCTTAGTGGATACATCGGGTTCATGCTCGGCCAAGAAAAATACCAATGTGCACCTCATTCTCCTTCTCCTTCTCCTTCCCTTTCCCCTTCTAAGTCTGCCCCCGCTGCTTCTTGTCTTCTTCACAGCCCGTCTTCTGCTTCCTCCATCACTCCTGGTCGTCAAGGGAATACTAGTGAAGGACAGAGGGAACTGTATTCCTGTTTGTCAACAACTCCATCCAAATCTGCTCGAACTTCTCTTTTCCAAGTCCCATCATCTGCTCCGCATAATACATCTGCTCATCAAGAGCACCTTGGGGACCGTGGAGGAGGGGAGAGGCCACTTTATTCCTCCTATCACTCGCATTCTCCTTCAAAAGCTGCCAGTACTTCTCTTTTTCAAGCCCCCTCTTCTGCTGCTCCTTCCACGTCTCTCACTCCGGATCGACCTTTTCAAGGTCACACATTTTTCACTTTCTCGGTTTCAGGCTTTGCATTCATGGTGATCAACTGTTTTTTGTTTTTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

286

Amino Acids

30.87

Weight (kDa)

6.66

Isoelectric Point (pI)

54.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HB_RdRP3_N PF26249 64 - 128 2.1e-08 RdRP3/4/5, N-terminal four helical bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021154)

Species Orthologous Gene IDs
malus_domestica MD02G1064500.v1.1 MD02G1064700.v1.1 MD15G1195200.v1.1
prunus_persica Prupe.7G221300_v2.0.a1
pyrus_communis pycom02g05080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 53
AccIII TCCGGA 1 cut(s) 766
AciI CCGC 8 cut(s) 53, 129, 173, 188, 194, 245, 450, 611
AclWI GGATC 2 cut(s) 12, 777
AcoI YGGCCR 1 cut(s) 376
AcsI RAATTY 1 cut(s) 318
AcvI CACGTG 1 cut(s) 184
AcyI GRCGYC 2 cut(s) 156, 255
AfaI GTAC 1 cut(s) 715
AfiI CCNNNNNNNGG 1 cut(s) 782
AgsI TTSAA 3 cut(s) 702, 728, 782
AhlI ACTAGT 1 cut(s) 518
AjiI CACGTC 1 cut(s) 756
AjnI CCWGG 1 cut(s) 499
AluBI AGCT 2 cut(s) 274, 707
AluI AGCT 2 cut(s) 274, 707
Alw21I GWGCWC 2 cut(s) 400, 639
Alw26I GTCTC 1 cut(s) 762
Alw44I GTGCAC 1 cut(s) 396
AlwI GGATC 2 cut(s) 12, 777
Aor13HI TCCGGA 1 cut(s) 766
AoxI GGCC 2 cut(s) 376, 665
ApaLI GTGCAC 1 cut(s) 396
ApeKI GCWGC 8 cut(s) 148, 190, 205, 241, 247, 452, 707, 743
ApoI RAATTY 1 cut(s) 318
AspLEI GCGC 2 cut(s) 137, 148
AspS9I GGNCC 1 cut(s) 646
AsuHPI GGTGA 2 cut(s) 326, 841
AvaII GGWCC 1 cut(s) 646
BaeGI GKGCMC 1 cut(s) 400
BbrPI CACGTG 1 cut(s) 184
BbsI GAAGAC 2 cut(s) 455, 471
Bbv12I GWGCWC 2 cut(s) 400, 639
BbvI GCAGC 8 cut(s) 135, 177, 217, 253, 259, 439, 694, 730
BccI CCATC 5 cut(s) 21, 83, 500, 571, 607
BciT130I CCWGG 1 cut(s) 501
BciVI GTATCC 1 cut(s) 350
BclI TGATCA 1 cut(s) 831
BcoDI GTCTC 1 cut(s) 762
BcuI ACTAGT 1 cut(s) 518
BfaI CTAG 1 cut(s) 519
BfoI RGCGCY 1 cut(s) 138
BfuI GTATCC 1 cut(s) 350
BmcAI AGTACT 1 cut(s) 715
Bme1390I CCNGG 1 cut(s) 501
Bme18I GGWCC 1 cut(s) 646
BmgBI CACGTC 1 cut(s) 756
BmgT120I GGNCC 1 cut(s) 646
BmiI GGNNCC 1 cut(s) 647
BmrFI CCNGG 1 cut(s) 501
BpiI GAAGAC 2 cut(s) 455, 471
BsaAI YACGTR 1 cut(s) 184
BsaHI GRCGYC 2 cut(s) 156, 255
BsaJI CCNNGG 3 cut(s) 310, 640, 649
BsaWI WCCGGW 1 cut(s) 766
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 1 cut(s) 782
Bse1I ACTGG 1 cut(s) 711
BseAI TCCGGA 1 cut(s) 766
BseBI CCWGG 1 cut(s) 501
BseDI CCNNGG 3 cut(s) 310, 640, 649
BseGI GGATG 2 cut(s) 61, 563
BseLI CCNNNNNNNGG 1 cut(s) 782
BseNI ACTGG 1 cut(s) 711
BseRI GAGGAG 3 cut(s) 299, 305, 669
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 8 cut(s) 135, 177, 217, 253, 259, 439, 694, 730
BseYI CCCAGC 2 cut(s) 274, 283
BsgI GTGCAG 2 cut(s) 164, 224
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 2 cut(s) 378, 667
BsiHKAI GWGCWC 2 cut(s) 400, 639
BsiSI CCGG 1 cut(s) 767
BslFI GGGAC 2 cut(s) 581, 659
BslI CCNNNNNNNGG 1 cut(s) 782
BsmAI GTCTC 1 cut(s) 762
BsmBI CGTCTC 1 cut(s) 762
BsmFI GGGAC 2 cut(s) 581, 659
BsmI GAATGC 2 cut(s) 691, 821
BsnI GGCC 2 cut(s) 378, 667
Bsp1286I GDGCHC 2 cut(s) 400, 639
Bsp13I TCCGGA 1 cut(s) 766
Bsp143I GATC 4 cut(s) 4, 339, 769, 831
BspACI CCGC 8 cut(s) 53, 129, 173, 188, 194, 245, 450, 611
BspANI GGCC 2 cut(s) 378, 667
BspEI TCCGGA 1 cut(s) 766
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 1 cut(s) 647
BspPI GGATC 2 cut(s) 12, 777
BsrI ACTGG 1 cut(s) 711
BssECI CCNNGG 3 cut(s) 310, 640, 649
BssMI GATC 4 cut(s) 4, 339, 769, 831
BssNI GRCGYC 2 cut(s) 156, 255
BssT1I CCWWGG 1 cut(s) 640
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 4 cut(s) 142, 540, 650, 839
Bst6I CTCTTC 1 cut(s) 742
BstACI GRCGYC 2 cut(s) 156, 255
BstBAI YACGTR 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 133
BstDEI CTNAG 2 cut(s) 350, 438
BstDSI CCRYGG 1 cut(s) 649
BstENI CCTNNNNNAGG 1 cut(s) 780
BstF5I GGATG 2 cut(s) 61, 563
BstFNI CGCG 1 cut(s) 53
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 2 cut(s) 137, 148
BstKTI GATC 4 cut(s) 7, 342, 772, 834
BstMAI GTCTC 1 cut(s) 762
BstMBI GATC 4 cut(s) 4, 339, 769, 831
BstMWI GCNNNNNNNGC 7 cut(s) 143, 187, 193, 202, 247, 280, 452
BstNI CCWGG 1 cut(s) 501
BstSCI CCNGG 1 cut(s) 499
BstSLI GKGCMC 1 cut(s) 400
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 8 cut(s) 135, 177, 217, 253, 259, 439, 694, 730
BstV2I GAAGAC 2 cut(s) 455, 471
BsuI GTATCC 1 cut(s) 350
BsuRI GGCC 2 cut(s) 378, 667
BtgI CCRYGG 1 cut(s) 649
BtrI CACGTC 1 cut(s) 756
BtsCI GGATG 2 cut(s) 61, 563
Cac8I GCNNGC 1 cut(s) 133
CfoI GCGC 2 cut(s) 137, 148
Cfr13I GGNCC 1 cut(s) 646
CseI GACGC 2 cut(s) 164, 263
Csp6I GTAC 1 cut(s) 714
CviAII CATG 2 cut(s) 370, 826
CviJI RGCY 9 cut(s) 244, 250, 274, 378, 474, 667, 707, 731, 816
CviKI_1 RGCY 9 cut(s) 244, 250, 274, 378, 474, 667, 707, 731, 816
CviQI GTAC 1 cut(s) 714
DdeI CTNAG 2 cut(s) 350, 438
DpnI GATC 4 cut(s) 6, 341, 771, 833
DpnII GATC 4 cut(s) 4, 339, 769, 831
EaeI YGGCCR 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 742
EarI CTCTTC 1 cut(s) 742
Eco130I CCWWGG 1 cut(s) 640
Eco47I GGWCC 1 cut(s) 646
Eco72I CACGTG 1 cut(s) 184
EcoNI CCTNNNNNAGG 1 cut(s) 780
EcoRII CCWGG 1 cut(s) 499
EcoT14I CCWWGG 1 cut(s) 640
ErhI CCWWGG 1 cut(s) 640
Esp3I CGTCTC 1 cut(s) 762
FaeI CATG 2 cut(s) 373, 829
FaiI YATR 5 cut(s) 224, 226, 371, 615, 827
FalI AAGNNNNNCTT 2 cut(s) 624, 656
FaqI GGGAC 2 cut(s) 581, 659
FatI CATG 2 cut(s) 369, 825
FauI CCCGC 1 cut(s) 457
FauNDI CATATG 1 cut(s) 224
FbaI TGATCA 1 cut(s) 831
FokI GGATG 2 cut(s) 68, 550
FspBI CTAG 1 cut(s) 519
GlaI GCGC 2 cut(s) 136, 147
GsaI CCCAGC 2 cut(s) 278, 287
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 2 cut(s) 378, 667
HapII CCGG 1 cut(s) 767
HgaI GACGC 2 cut(s) 164, 263
HhaI GCGC 2 cut(s) 137, 148
Hin1I GRCGYC 2 cut(s) 156, 255
Hin1II CATG 2 cut(s) 373, 829
Hin6I GCGC 2 cut(s) 135, 146
HinP1I GCGC 2 cut(s) 135, 146
HincII GTYRAC 1 cut(s) 555
HindII GTYRAC 1 cut(s) 555
HpaII CCGG 1 cut(s) 767
HphI GGTGA 2 cut(s) 326, 841
Hpy166II GTNNAC 2 cut(s) 398, 555
Hpy188I TCNGA 2 cut(s) 21, 344
Hpy188III TCNNGA 2 cut(s) 632, 767
Hpy8I GTNNAC 2 cut(s) 398, 555
Hpy99I CGWCG 1 cut(s) 158
HpyCH4III ACNGT 4 cut(s) 142, 540, 650, 839
HpyCH4IV ACGT 2 cut(s) 183, 755
HpyCH4V TGCA 5 cut(s) 30, 181, 205, 398, 821
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 187, 193, 202, 247, 280, 452
HpyF3I CTNAG 2 cut(s) 350, 438
HpySE526I ACGT 2 cut(s) 183, 755
Hsp92I GRCGYC 2 cut(s) 156, 255
Hsp92II CATG 2 cut(s) 373, 829
HspAI GCGC 2 cut(s) 135, 146
Kpn2I TCCGGA 1 cut(s) 766
Ksp22I TGATCA 1 cut(s) 831
Kzo9I GATC 4 cut(s) 4, 339, 769, 831
LmnI GCTCC 2 cut(s) 613, 751
Lsp1109I GCAGC 8 cut(s) 135, 177, 217, 253, 259, 439, 694, 730
MaeI CTAG 1 cut(s) 519
MaeII ACGT 2 cut(s) 183, 755
MaeIII GTNAC 2 cut(s) 38, 785
MalI GATC 4 cut(s) 6, 341, 771, 833
MboI GATC 4 cut(s) 4, 339, 769, 831
MboII GAAGA 5 cut(s) 70, 455, 458, 471, 729
MhlI GDGCHC 2 cut(s) 400, 639
MluCI AATT 1 cut(s) 318
MroI TCCGGA 1 cut(s) 766
MspA1I CMGCKG 2 cut(s) 190, 452
MspI CCGG 1 cut(s) 767
MspR9I CCNGG 1 cut(s) 501
Mva1269I GAATGC 2 cut(s) 691, 821
MvaI CCWGG 1 cut(s) 501
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 7 cut(s) 143, 187, 193, 202, 247, 280, 452
NdeI CATATG 1 cut(s) 224
NdeII GATC 4 cut(s) 4, 339, 769, 831
NlaIII CATG 2 cut(s) 373, 829
NlaIV GGNNCC 1 cut(s) 647
NmeAIII GCCGAG 2 cut(s) 283, 354
NmuCI GTSAC 1 cut(s) 785
PctI GAATGC 2 cut(s) 691, 821
PmaCI CACGTG 1 cut(s) 184
PmlI CACGTG 1 cut(s) 184
Ppu21I YACGTR 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 499
PspCI CACGTG 1 cut(s) 184
PspFI CCCAGC 2 cut(s) 274, 283
PspGI CCWGG 1 cut(s) 499
PspN4I GGNNCC 1 cut(s) 647
PspPI GGNCC 1 cut(s) 646
RsaI GTAC 1 cut(s) 715
RsaNI GTAC 1 cut(s) 714
Sau3AI GATC 4 cut(s) 4, 339, 769, 831
Sau96I GGNCC 1 cut(s) 646
ScaI AGTACT 1 cut(s) 715
ScrFI CCNGG 1 cut(s) 501
SduI GDGCHC 2 cut(s) 400, 639
SetI ASST 9 cut(s) 186, 204, 276, 403, 642, 709, 758, 778, 787
SinI GGWCC 1 cut(s) 646
SpeI ACTAGT 1 cut(s) 518
Sse9I AATT 1 cut(s) 318
SsiI CCGC 8 cut(s) 53, 129, 173, 188, 194, 245, 450, 611
SspMI CTAG 1 cut(s) 519
StyD4I CCNGG 1 cut(s) 499
StyI CCWWGG 1 cut(s) 640
TaaI ACNGT 4 cut(s) 142, 540, 650, 839
TaiI ACGT 2 cut(s) 186, 758
TaqI TCGA 4 cut(s) 66, 303, 577, 772
TasI AATT 1 cut(s) 318
TatI WGTACW 1 cut(s) 713
TauI GCSGC 3 cut(s) 190, 196, 247
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 8 cut(s) 148, 190, 205, 241, 247, 452, 707, 743
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 3 cut(s) 87, 358, 814
VneI GTGCAC 1 cut(s) 396
VpaK11BI GGWCC 1 cut(s) 646
XagI CCTNNNNNAGG 1 cut(s) 780
XapI RAATTY 1 cut(s) 318
XspI CTAG 1 cut(s) 519
ZrmI AGTACT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.