MD02G1096000.v1.1

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013113
Physical Location & Seq
Forward (+)
7575838 .. 7581628
5791 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1096000.v1.1.491

Sequence Viewer

Length: 1770 bp
ATGGTTTTCTGGTTTGCTGAGAGCAACAACCTTCCCTTGAATTGCACTCAAACTTGCCCATTTCACAAACCAAATCCATCACTCTCAATCTCCACCCAATTGGGTCCAATCCATTCCCTTCAACCAAACACAACCAAATCTCAGAAATCCCATGTCCTCAAAAATCAAGCTCACCCATTTCCCCAACCTCCAATCCCCACCTCCCCTCGATCTTCTGAGCCGAATCGGCGGAACCCAGTAGCCCATTGCTCAGAAAGATCACTGCTTTTGGGTGGTTGCAGAGTCGTGTTGACCATGTTGGAGGCCTTGCCGCTAGGACGGTTCCACCACCAGAGGCTTGACCTGAAGCGGTGCTTCCCCACCTTCTTGTCCTCCCACAAAACCCTCTTCACCGTGCTCTGGATCGCCGCTTTCGCCTCGGTTTTCGTGTGGCAGAGGAACATTGTAGATGGGTTTTCGATATTCAGGCGAGCTCCGGCGAGGGCGATGCCGAAGCTGAGGCCGGTGGCTTTCAATTTGACTGAATTTGGGGCTGTGGGCGATGGGGTTACTTTGAACACTGAGGCGTTTGAGAAAGCGGTGCTGGCTATTTCTAAGCTGGGGAAGAAAGGCGGTGGGCAGCTCAATGTGCCTCCGGGACGGTGGCTAACGGCGCCGTTTAACCTCACTAGTCACATGACTCTGTTTCTTGCTGAAGATGCTGAGATACTTGGAATTCAGGATGAGAAGTATTGGCCCTTGATGCCTCCGTTGCCTTCATACGGGTATGGGAGAGAGCATCATGGGCCTCGGTTTGGAAGTCTGATTCATGGTCAAAATCTTAAAGATATTGTTATAACAGGGCATAATGGTACCATAAATGGACAGGGTCAAACATGGTGGAAGAAGTACCGCCAGAAGCTTCTCAACAACACTCGGGGTCCACTTGTTCAGATTATGTGGTCTAGCGACATTTTGATCAGTAATATAACATTGCGTGATTCTCCTTTTTGGACACTTCATCCATATGACTGCAAGAACATTACTGTTAGAAATGTTACAATTTTGGCTCCCGTACTTGAAGCTCCAAATACTGATGGAATAGATCCTGATTCATGTGAGGATATGGTGATTGAGGACTGCTACATTAGTGTAGGGGATGATGGGATTGCAATAAAGAGTGGCTGGGATCAGTATGGTATTTCTTACGGACGGCCATCGAAGAATATACTCATTAGAAATCTTGTTGTCCGCTCTATGGTCAGTGCTGGTGTATCAATTGGCAGTGAGATGTCTGGTGGGGTTTCGAATGTCACTGTGGAGAACCTGCTTGTGTGGAGCTCAAGGCGTGCTGTTCGGATCAAGACTGCCCCTGGAAGAGGCGGATATGTGCGCCAAATAACCTATAGGAATCTAACATTTGACAATGTTCGAGTTGGAATTGTTATCAAGACAGACTACAATGAGCACCCCGATGAGGGGTATGATCGAAAGGCTCTTCCAGTGCTTGAGGACATAAGCTTCACAAGCGTCCATGGACAGGGAGTTCGCGTGCCTGTCCGTATCCATGGGAGTGAAGAGATTCCAGTGAGAAATGTGACTTTCCGGGACATGTCCGTTGGTCTAACATACAAAAAGAAGCACATTTTCCAGTGTGCCTTTGTTCAAGGTCGCGTGATAGGGACCATCTTCCCTGCCCCATGCGAGAATCTTGACCGGTACGACGAGCAAGGAAAGCTGGTGAATCACTCTGCATCCCAGAACATGACAGACATAGATTATGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

590

Amino Acids

65.83

Weight (kDa)

9.15

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 266 - 548 3.2e-47 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19170 AT3G42950
fragaria_vesca FvH4_1g09050 FvH4_1g09050
malus_domestica MD02G1096000.v1.1
prunus_persica Prupe.7G195000_v2.0.a1
pyrus_communis pycom02g07580 pycom15g19530
rosa_chinensis RchiOBHm_Chr2g0095471
rosa_laevigata RLG00000016570
rosa_multiflora Rmu_sc0006076.1_g000011
rosa_roxburghii Rroxscaffold_2G00146310
rosa_rugosa Rorug02G0051400.1
rosa_samantha Rh2AG099400 Rh2BG100700 Rh2CG102800 Rh2DG100300
rosa_wichuraiana Rw2G007530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 836
Acc36I ACCTGC 1 cut(s) 1314
Acc65I GGTACC 1 cut(s) 851
AccB1I GGYRCC 2 cut(s) 652, 851
AccBSI CCGCTC 1 cut(s) 1233
AccII CGCG 2 cut(s) 1530, 1653
AciI CCGC 9 cut(s) 229, 311, 349, 408, 578, 612, 892, 1231, 1362
AclWI GGATC 4 cut(s) 410, 1079, 1176, 1346
AcoI YGGCCR 1 cut(s) 1193
AcsI RAATTY 2 cut(s) 524, 714
AcuI CTGAAG 2 cut(s) 365, 714
AcyI GRCGYC 1 cut(s) 653
AfaI GTAC 4 cut(s) 853, 890, 1056, 1700
AfiI CCNNNNNNNGG 9 cut(s) 399, 761, 794, 1237, 1358, 1456, 1457, 1458, 1519
AflIII ACRYGT 1 cut(s) 1590
AgeI ACCGGT 1 cut(s) 1695
AgsI TTSAA 6 cut(s) 40, 122, 514, 556, 1061, 1646
AhlI ACTAGT 1 cut(s) 668
AjnI CCWGG 1 cut(s) 1351
AloI GAACNNNNNNTCC 2 cut(s) 1509, 1541
Alw21I GWGCWC 4 cut(s) 399, 475, 1322, 1449
AlwI GGATC 4 cut(s) 410, 1079, 1176, 1346
Ama87I CYCGRG 1 cut(s) 915
AoxI GGCC 5 cut(s) 303, 500, 734, 785, 1193
ApeKI GCWGC 1 cut(s) 619
ApoI RAATTY 2 cut(s) 524, 714
ArsI GACNNNNNNTTYG 2 cut(s) 1509, 1541
AsiGI ACCGGT 1 cut(s) 1695
Asp700I GAANNNNTTC 1 cut(s) 1560
Asp718I GGTACC 1 cut(s) 851
AspLEI GCGC 2 cut(s) 655, 1374
AspS9I GGNCC 5 cut(s) 104, 735, 785, 920, 1662
AsuC2I CCSGG 2 cut(s) 636, 1586
AsuHPI GGTGA 4 cut(s) 164, 382, 1120, 1732
AsuII TTCGAA 1 cut(s) 1286
AvaI CYCGRG 1 cut(s) 915
AvaII GGWCC 3 cut(s) 104, 920, 1662
BanI GGYRCC 2 cut(s) 652, 851
BanII GRGCYC 2 cut(s) 475, 1322
Bbv12I GWGCWC 4 cut(s) 399, 475, 1322, 1449
BbvCI CCTCAGC 1 cut(s) 497
BbvI GCAGC 1 cut(s) 631
BccI CCATC 7 cut(s) 85, 443, 536, 1070, 1136, 1204, 1673
BceAI ACGGC 3 cut(s) 640, 666, 1208
BcgI CGANNNNNNTGC 2 cut(s) 469, 503
BciT130I CCWGG 1 cut(s) 1353
BciVI GTATCC 1 cut(s) 1553
BclI TGATCA 1 cut(s) 957
BcnI CCSGG 2 cut(s) 636, 1586
BcuI ACTAGT 1 cut(s) 668
BfaI CTAG 3 cut(s) 314, 669, 945
BfmI CTRYAG 1 cut(s) 1384
BfoI RGCGCY 1 cut(s) 656
BfuAI ACCTGC 1 cut(s) 1314
BfuI GTATCC 1 cut(s) 1553
BglI GCCNNNNNGGC 1 cut(s) 226
BisI GCNGC 3 cut(s) 311, 408, 620
BlsI GCNGC 3 cut(s) 312, 409, 621
Bme1390I CCNGG 3 cut(s) 636, 1353, 1586
Bme18I GGWCC 3 cut(s) 104, 920, 1662
BmeT110I CYCGRG 1 cut(s) 915
BmgT120I GGNCC 5 cut(s) 104, 735, 785, 920, 1662
BmiI GGNNCC 8 cut(s) 105, 233, 323, 654, 853, 921, 1050, 1663
BmrFI CCNGG 3 cut(s) 636, 1353, 1586
BmrI ACTGGG 1 cut(s) 230
BmsI GCATC 5 cut(s) 477, 688, 732, 787, 1742
BmuI ACTGGG 1 cut(s) 230
Bpu10I CCTNAGC 1 cut(s) 497
Bpu14I TTCGAA 1 cut(s) 1286
BpuEI CTTGAG 2 cut(s) 1306, 1508
BpuMI CCSGG 2 cut(s) 636, 1586
BsaHI GRCGYC 1 cut(s) 653
BsaJI CCNNGG 5 cut(s) 417, 788, 1351, 1513, 1546
BsaWI WCCGGW 1 cut(s) 1695
BsaXI ACNNNNNCTCC 2 cut(s) 1515, 1545
Bsc4I CCNNNNNNNGG 9 cut(s) 399, 761, 794, 1237, 1358, 1456, 1457, 1458, 1519
Bse118I RCCGGY 2 cut(s) 502, 1695
Bse1I ACTGG 4 cut(s) 236, 1481, 1565, 1630
Bse3DI GCAATG 2 cut(s) 244, 971
BseBI CCWGG 1 cut(s) 1353
BseDI CCNNGG 5 cut(s) 417, 788, 1351, 1513, 1546
BseGI GGATG 4 cut(s) 727, 1000, 1144, 1733
BseLI CCNNNNNNNGG 9 cut(s) 399, 761, 794, 1237, 1358, 1456, 1457, 1458, 1519
BseMI GCAATG 2 cut(s) 244, 971
BseMII CTCAG 7 cut(s) 9, 155, 207, 264, 488, 552, 693
BseNI ACTGG 4 cut(s) 236, 1481, 1565, 1630
BseXI GCAGC 1 cut(s) 631
BseYI CCCAGC 2 cut(s) 598, 1164
Bsh1236I CGCG 2 cut(s) 1530, 1653
BshFI GGCC 5 cut(s) 305, 502, 736, 787, 1195
BshNI GGYRCC 2 cut(s) 652, 851
BshTI ACCGGT 1 cut(s) 1695
BsiHKAI GWGCWC 4 cut(s) 399, 475, 1322, 1449
BsiHKCI CYCGRG 1 cut(s) 915
BsiSI CCGG 5 cut(s) 476, 503, 635, 1585, 1696
BslFI GGGAC 3 cut(s) 651, 1601, 1675
BslI CCNNNNNNNGG 9 cut(s) 399, 761, 794, 1237, 1358, 1456, 1457, 1458, 1519
BsmFI GGGAC 3 cut(s) 651, 1601, 1675
BsnI GGCC 5 cut(s) 305, 502, 736, 787, 1195
BsoBI CYCGRG 1 cut(s) 915
Bsp119I TTCGAA 1 cut(s) 1286
Bsp1286I GDGCHC 4 cut(s) 399, 475, 1322, 1449
Bsp143I GATC 8 cut(s) 209, 257, 402, 957, 1084, 1168, 1338, 1465
Bsp19I CCATGG 2 cut(s) 1513, 1546
BspACI CCGC 9 cut(s) 229, 311, 349, 408, 578, 612, 892, 1231, 1362
BspANI GGCC 5 cut(s) 305, 502, 736, 787, 1195
BspCNI CTCAG 7 cut(s) 10, 154, 208, 263, 489, 553, 694
BspFNI CGCG 2 cut(s) 1530, 1653
BspLI GGNNCC 8 cut(s) 105, 233, 323, 654, 853, 921, 1050, 1663
BspMI ACCTGC 1 cut(s) 1314
BspPI GGATC 4 cut(s) 410, 1079, 1176, 1346
BspQI GCTCTTC 1 cut(s) 1482
BspT104I TTCGAA 1 cut(s) 1286
BspT107I GGYRCC 2 cut(s) 652, 851
BsrBI CCGCTC 1 cut(s) 1233
BsrDI GCAATG 2 cut(s) 244, 971
BsrFI RCCGGY 2 cut(s) 502, 1695
BsrI ACTGG 4 cut(s) 236, 1481, 1565, 1630
BssAI RCCGGY 2 cut(s) 502, 1695
BssECI CCNNGG 5 cut(s) 417, 788, 1351, 1513, 1546
BssMI GATC 8 cut(s) 209, 257, 402, 957, 1084, 1168, 1338, 1465
BssNI GRCGYC 1 cut(s) 653
BssT1I CCWWGG 2 cut(s) 1513, 1546
Bst2UI CCWGG 1 cut(s) 1353
Bst4CI ACNGT 5 cut(s) 321, 394, 642, 1027, 1297
Bst6I CTCTTC 4 cut(s) 392, 1351, 1482, 1551
BstACI GRCGYC 1 cut(s) 653
BstBI TTCGAA 1 cut(s) 1286
BstC8I GCNNGC 4 cut(s) 471, 585, 1329, 1532
BstDEI CTNAG 8 cut(s) 18, 141, 216, 250, 497, 561, 594, 702
BstDSI CCRYGG 2 cut(s) 1513, 1546
BstENI CCTNNNNNAGG 1 cut(s) 1356
BstF5I GGATG 4 cut(s) 727, 1000, 1144, 1733
BstFNI CGCG 2 cut(s) 1530, 1653
BstH2I RGCGCY 1 cut(s) 656
BstHHI GCGC 2 cut(s) 655, 1374
BstKTI GATC 8 cut(s) 212, 260, 405, 960, 1087, 1171, 1341, 1468
BstMBI GATC 8 cut(s) 209, 257, 402, 957, 1084, 1168, 1338, 1465
BstNI CCWGG 1 cut(s) 1353
BstNSI RCATGY 1 cut(s) 1594
BstSCI CCNGG 3 cut(s) 634, 1351, 1584
BstSFI CTRYAG 1 cut(s) 1384
BstUI CGCG 2 cut(s) 1530, 1653
BstV1I GCAGC 1 cut(s) 631
BstX2I RGATCY 1 cut(s) 1084
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 1 cut(s) 1084
BsuI GTATCC 1 cut(s) 1553
BsuRI GGCC 5 cut(s) 305, 502, 736, 787, 1195
BtgI CCRYGG 2 cut(s) 1513, 1546
BtgZI GCGATG 2 cut(s) 500, 555
BtsCI GGATG 4 cut(s) 727, 1000, 1144, 1733
BtsI GCAGTG 2 cut(s) 260, 1270
BtsIMutI CAGTG 8 cut(s) 260, 558, 1249, 1270, 1293, 1488, 1572, 1637
BveI ACCTGC 1 cut(s) 1314
Cac8I GCNNGC 4 cut(s) 471, 585, 1329, 1532
CfoI GCGC 2 cut(s) 655, 1374
Cfr10I RCCGGY 2 cut(s) 502, 1695
Cfr13I GGNCC 5 cut(s) 104, 735, 785, 920, 1662
CseI GACGC 1 cut(s) 1498
Csp6I GTAC 4 cut(s) 852, 889, 1055, 1699
CspAI ACCGGT 1 cut(s) 1695
CspCI CAANNNNNGTGG 2 cut(s) 860, 895
CviQI GTAC 4 cut(s) 852, 889, 1055, 1699
DdeI CTNAG 8 cut(s) 18, 141, 216, 250, 497, 561, 594, 702
DinI GGCGCC 1 cut(s) 654
DpnI GATC 8 cut(s) 211, 259, 404, 959, 1086, 1170, 1340, 1467
DpnII GATC 8 cut(s) 209, 257, 402, 957, 1084, 1168, 1338, 1465
EaeI YGGCCR 1 cut(s) 1193
Eam1104I CTCTTC 4 cut(s) 392, 1351, 1482, 1551
EarI CTCTTC 4 cut(s) 392, 1351, 1482, 1551
EciI GGCGGA 2 cut(s) 244, 1377
Ecl136II GAGCTC 2 cut(s) 473, 1320
Eco130I CCWWGG 2 cut(s) 1513, 1546
Eco147I AGGCCT 1 cut(s) 305
Eco24I GRGCYC 2 cut(s) 475, 1322
Eco47I GGWCC 3 cut(s) 104, 920, 1662
Eco53kI GAGCTC 2 cut(s) 473, 1320
Eco57I CTGAAG 2 cut(s) 365, 714
Eco88I CYCGRG 1 cut(s) 915
EcoICRI GAGCTC 2 cut(s) 473, 1320
EcoNI CCTNNNNNAGG 1 cut(s) 1356
EcoRI GAATTC 1 cut(s) 714
EcoRII CCWGG 1 cut(s) 1351
EcoT14I CCWWGG 2 cut(s) 1513, 1546
EcoT38I GRGCYC 2 cut(s) 475, 1322
EgeI GGCGCC 1 cut(s) 654
EheI GGCGCC 1 cut(s) 654
ErhI CCWWGG 2 cut(s) 1513, 1546
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 3 cut(s) 651, 1601, 1675
FauNDI CATATG 1 cut(s) 1006
FbaI TGATCA 1 cut(s) 957
Fnu4HI GCNGC 3 cut(s) 311, 408, 620
FokI GGATG 4 cut(s) 734, 987, 1151, 1720
FriOI GRGCYC 2 cut(s) 475, 1322
Fsp4HI GCNGC 3 cut(s) 311, 408, 620
FspBI CTAG 3 cut(s) 314, 669, 945
GlaI GCGC 2 cut(s) 654, 1373
GluI GCNGC 3 cut(s) 311, 408, 620
GsaI CCCAGC 2 cut(s) 602, 1168
HaeII RGCGCY 1 cut(s) 656
HaeIII GGCC 5 cut(s) 305, 502, 736, 787, 1195
HapII CCGG 5 cut(s) 476, 503, 635, 1585, 1696
HgaI GACGC 1 cut(s) 1498
HhaI GCGC 2 cut(s) 655, 1374
Hin1I GRCGYC 1 cut(s) 653
Hin6I GCGC 2 cut(s) 653, 1372
HinP1I GCGC 2 cut(s) 653, 1372
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HindIII AAGCTT 2 cut(s) 899, 1498
HpaII CCGG 5 cut(s) 476, 503, 635, 1585, 1696
HphI GGTGA 4 cut(s) 164, 382, 1120, 1732
Hpy166II GTNNAC 2 cut(s) 291, 923
Hpy188I TCNGA 6 cut(s) 144, 217, 253, 804, 933, 1338
Hpy188III TCNNGA 6 cut(s) 400, 719, 1088, 1342, 1429, 1691
Hpy8I GTNNAC 2 cut(s) 291, 923
Hpy99I CGWCG 1 cut(s) 1706
HpyAV CCTTC 4 cut(s) 41, 128, 373, 765
HpyCH4III ACNGT 5 cut(s) 321, 394, 642, 1027, 1297
HpyCH4V TGCA 5 cut(s) 45, 279, 1014, 1151, 1733
HpyF3I CTNAG 8 cut(s) 18, 141, 216, 250, 497, 561, 594, 702
Hsp92I GRCGYC 1 cut(s) 653
HspAI GCGC 2 cut(s) 653, 1372
KasI GGCGCC 1 cut(s) 652
KpnI GGTACC 1 cut(s) 855
Ksp22I TGATCA 1 cut(s) 957
Kzo9I GATC 8 cut(s) 209, 257, 402, 957, 1084, 1168, 1338, 1465
LguI GCTCTTC 1 cut(s) 1482
LmnI GCTCC 4 cut(s) 478, 1054, 1069, 1317
Lsp1109I GCAGC 1 cut(s) 631
LweI GCATC 5 cut(s) 477, 688, 732, 787, 1742
MaeI CTAG 3 cut(s) 314, 669, 945
MaeIII GTNAC 5 cut(s) 547, 671, 1036, 1291, 1576
MalI GATC 8 cut(s) 211, 259, 404, 959, 1086, 1170, 1340, 1467
MbiI CCGCTC 1 cut(s) 1233
MboI GATC 8 cut(s) 209, 257, 402, 957, 1084, 1168, 1338, 1465
MfeI CAATTG 2 cut(s) 98, 1257
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 4 cut(s) 399, 475, 1322, 1449
MluCI AATT 8 cut(s) 40, 98, 514, 524, 714, 1041, 1257, 1419
Mly113I GGCGCC 1 cut(s) 653
MlyI GAGTC 2 cut(s) 291, 673
MmeI TCCRAC 2 cut(s) 279, 1396
MroXI GAANNNNTTC 1 cut(s) 1560
MseI TTAA 2 cut(s) 660, 822
MslI CAYNNNNRTG 3 cut(s) 1005, 1452, 1551
MspI CCGG 5 cut(s) 476, 503, 635, 1585, 1696
MspR9I CCNGG 3 cut(s) 636, 1353, 1586
MunI CAATTG 2 cut(s) 98, 1257
MvaI CCWGG 1 cut(s) 1353
MvnI CGCG 2 cut(s) 1530, 1653
NarI GGCGCC 1 cut(s) 653
NciI CCSGG 2 cut(s) 636, 1586
NcoI CCATGG 2 cut(s) 1513, 1546
NdeI CATATG 1 cut(s) 1006
NdeII GATC 8 cut(s) 209, 257, 402, 957, 1084, 1168, 1338, 1465
NlaIV GGNNCC 8 cut(s) 105, 233, 323, 654, 853, 921, 1050, 1663
NmuCI GTSAC 3 cut(s) 671, 1291, 1576
NspI RCATGY 1 cut(s) 1594
NspV TTCGAA 1 cut(s) 1286
PceI AGGCCT 1 cut(s) 305
PciI ACATGT 1 cut(s) 1590
PciSI GCTCTTC 1 cut(s) 1482
PdmI GAANNNNTTC 1 cut(s) 1560
PfeI GAWTC 8 cut(s) 223, 805, 980, 1091, 1390, 1561, 1687, 1723
PflFI GACNNNGTC 1 cut(s) 867
PfoI TCCNGGA 2 cut(s) 634, 1584
PinAI ACCGGT 1 cut(s) 1695
PkrI GCNGC 3 cut(s) 312, 409, 621
PleI GAGTC 2 cut(s) 290, 673
PluTI GGCGCC 1 cut(s) 656
PpsI GAGTC 2 cut(s) 290, 673
PscI ACATGT 1 cut(s) 1590
PsiI TTATAA 1 cut(s) 836
Psp124BI GAGCTC 2 cut(s) 475, 1322
Psp6I CCWGG 1 cut(s) 1351
PspFI CCCAGC 2 cut(s) 598, 1164
PspGI CCWGG 1 cut(s) 1351
PspN4I GGNNCC 8 cut(s) 105, 233, 323, 654, 853, 921, 1050, 1663
PspPI GGNCC 5 cut(s) 104, 735, 785, 920, 1662
PsuI RGATCY 1 cut(s) 1084
PsyI GACNNNGTC 1 cut(s) 867
RsaI GTAC 4 cut(s) 853, 890, 1056, 1700
RsaNI GTAC 4 cut(s) 852, 889, 1055, 1699
RseI CAYNNNNRTG 3 cut(s) 1005, 1452, 1551
SacI GAGCTC 2 cut(s) 475, 1322
SapI GCTCTTC 1 cut(s) 1482
SaqAI TTAA 2 cut(s) 660, 822
SatI GCNGC 3 cut(s) 311, 408, 620
Sau3AI GATC 8 cut(s) 209, 257, 402, 957, 1084, 1168, 1338, 1465
Sau96I GGNCC 5 cut(s) 104, 735, 785, 920, 1662
SchI GAGTC 2 cut(s) 291, 673
ScrFI CCNGG 3 cut(s) 636, 1353, 1586
SduI GDGCHC 4 cut(s) 399, 475, 1322, 1449
SfaNI GCATC 5 cut(s) 477, 688, 732, 787, 1742
SfcI CTRYAG 1 cut(s) 1384
SfoI GGCGCC 1 cut(s) 654
SfuI TTCGAA 1 cut(s) 1286
SinI GGWCC 3 cut(s) 104, 920, 1662
SmiMI CAYNNNNRTG 3 cut(s) 1005, 1452, 1551
SmlI CTYRAG 2 cut(s) 1321, 1487
SmoI CTYRAG 2 cut(s) 1321, 1487
SpeI ACTAGT 1 cut(s) 668
Sse9I AATT 8 cut(s) 40, 98, 514, 524, 714, 1041, 1257, 1419
SseBI AGGCCT 1 cut(s) 305
SsiI CCGC 9 cut(s) 229, 311, 349, 408, 578, 612, 892, 1231, 1362
SspDI GGCGCC 1 cut(s) 652
SspMI CTAG 3 cut(s) 314, 669, 945
SstI GAGCTC 2 cut(s) 475, 1322
StuI AGGCCT 1 cut(s) 305
StyD4I CCNGG 3 cut(s) 634, 1351, 1584
StyI CCWWGG 2 cut(s) 1513, 1546
TaaI ACNGT 5 cut(s) 321, 394, 642, 1027, 1297
TaqI TCGA 6 cut(s) 208, 458, 1199, 1286, 1411, 1468
TasI AATT 8 cut(s) 40, 98, 514, 524, 714, 1041, 1257, 1419
TauI GCSGC 2 cut(s) 313, 410
TfiI GAWTC 8 cut(s) 223, 805, 980, 1091, 1390, 1561, 1687, 1723
Tru1I TTAA 2 cut(s) 660, 822
Tru9I TTAA 2 cut(s) 660, 822
TscAI CASTG 8 cut(s) 267, 565, 1249, 1270, 1300, 1488, 1572, 1637
TseFI GTSAC 3 cut(s) 671, 1291, 1576
TseI GCWGC 1 cut(s) 619
Tsp45I GTSAC 3 cut(s) 671, 1291, 1576
TspDTI ATGAA 4 cut(s) 747, 797, 989, 1083
TspGWI ACGGA 4 cut(s) 738, 1203, 1529, 1585
TspRI CASTG 8 cut(s) 267, 565, 1249, 1270, 1300, 1488, 1572, 1637
Tth111I GACNNNGTC 1 cut(s) 867
VpaK11BI GGWCC 3 cut(s) 104, 920, 1662
XagI CCTNNNNNAGG 1 cut(s) 1356
XapI RAATTY 2 cut(s) 524, 714
XceI RCATGY 1 cut(s) 1594
XmnI GAANNNNTTC 1 cut(s) 1560
XspI CTAG 3 cut(s) 314, 669, 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.