Rh2DG100300

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013113
Physical Location & Seq
Forward (+)
8044684 .. 8049749
5066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG100300.1

Sequence Viewer

Length: 1476 bp
ATGGTGGAGACCTTACCCTTGGGGCGGTTCCACCACCATAGACTCGACCTGAAGCGGTGGCTCCCTTCCTTCTTGTCCTCGCACAAAACCCTCTTCACCCTTCTCTGGATCGCCGCCTTCGCATCGGTTTTCGTGTGGCAGAGGAACATTGTAGATGGCTTCTCGTTTTTCCGGCGAGTTCCGGCGAGAGCCATGCCGAGGCTGAGACCGGCGGCGTTTAACTTGACGGATTTTGGGGCGGTGGGTGATGGTGTTACTTTGAACACCGAGGCGTTTGAGAAGGCTGTGTTGGCTATTTCAAAGCTGGGGAAGAAGGGTGGTGGTCAGCTCAATGTGCCTCCTGGGCGGTGGCTCACGGCGCCGTTTAATCTCACTAGTCATATGACTCTGTTTCTTGCTGAAGATGCTGAGATACTTGGAATGCAGGATGAGAAGTATTGGCCTTTGATGCCTCCACTGCCTTCATATGGGTATGGAAGAGAGCACCCTGGGCCTCGGTATGGAAGCTTGATTCATGGTCAAAATCTTAAAGATATTGTTATAACAGGACATAATGGTACCATTAATGGTCAGGGTCAAACATGGTGGAAGAAGTATCGCCAGAAGCTTCTCAATCACACGCGAGGTCCACTTGTTCAGATTATGTGGTCAAGCGACATTGTAATCACTAATATAACTTTACGAGATTCTCCTTTTTGGACACTTCATCCATATGACTGCAAGAATATTACAATTAGAAATGTTACAATCCTGGCTCCCATATTTGAAGCTCCAAATACTGATGGAATAGATCCTGATTCATGTGAGGATATGTTGATTGAGGACTGTTACATAAGTGTGGGGGACGACGGAATTGCAATAAAGAGTGGCTGGGATCAGTACGGAATTGCTTACGGACGGCCATCAAAAAATATACTCATTCGTAACCTTGTGGTCCGCTCTATGGTCAGCGCTGGCATATCAATAGGCAGTGAGATGTCTGGTGGAGTTTCAAATGTCACGGTGGAAAACCTGCTTGTGTGGAGCTCAAGGCGTGCTGTTCGGATCAAGACCGCCCCTGGAAGAGGTGGATATGTGCGTCACATAACCTACAGGAATCTGACGTTTGACAATGTTCGAGTTGGAATAGTTATCAAGACAGACTACAATGAGCACCCTGATGATGGCTACGATCGAAAGGCTTTTCCGGTGCTTAGGGACATAAGCTTCACAAGCATCCACGGACAGGGAGTTCGCGTGCCTGTCCGTATCCATGGGAGTGAAGAGATTCCGATAAGAAATGTGACTTTTCGAGATATGTCAGTGGGGATTACATACAAAAAGAAGCATATTTTCCAGTGTGCCTTTGTTCAAGGTCGTGTAATAGGGACCATCTTCCCTTCTCCTTGTGAAAATCTTGACCGGTATGATGAGCAAGAAAAGCTGGTGAAGCACTCTGTCTCCCAGAACATGACAGATATAGATTATGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

491

Amino Acids

55.43

Weight (kDa)

9.1

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 169 - 450 2e-47 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19170 AT3G42950
fragaria_vesca FvH4_1g09050 FvH4_1g09050
malus_domestica MD02G1096000.v1.1
prunus_persica Prupe.7G195000_v2.0.a1
pyrus_communis pycom02g07580 pycom15g19530
rosa_chinensis RchiOBHm_Chr2g0095471
rosa_laevigata RLG00000016570
rosa_multiflora Rmu_sc0006076.1_g000011
rosa_roxburghii Rroxscaffold_2G00146310
rosa_rugosa Rorug02G0051400.1
rosa_samantha Rh2AG099400 Rh2BG100700 Rh2CG102800 Rh2DG100300
rosa_wichuraiana Rw2G007530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 542
Acc36I ACCTGC 1 cut(s) 1020
Acc65I GGTACC 1 cut(s) 557
AccB1I GGYRCC 2 cut(s) 358, 557
AccBSI CCGCTC 1 cut(s) 939
AccII CGCG 2 cut(s) 622, 1236
AciI CCGC 8 cut(s) 25, 55, 114, 212, 239, 346, 937, 1053
AclWI GGATC 4 cut(s) 116, 785, 882, 1052
AcoI YGGCCR 1 cut(s) 899
AcuI CTGAAG 2 cut(s) 71, 420
AcyI GRCGYC 1 cut(s) 359
AfaI GTAC 2 cut(s) 559, 881
AfeI AGCGCT 1 cut(s) 952
AfiI CCNNNNNNNGG 9 cut(s) 24, 105, 198, 467, 500, 943, 1064, 1163, 1225
AgeI ACCGGT 1 cut(s) 1401
AgsI TTSAA 5 cut(s) 262, 300, 767, 993, 1352
AhlI ACTAGT 1 cut(s) 374
AjnI CCWGG 4 cut(s) 340, 487, 750, 1057
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AloI GAACNNNNNNTCC 2 cut(s) 1215, 1247
AluBI AGCT 8 cut(s) 304, 328, 507, 607, 770, 1026, 1206, 1423
AluI AGCT 8 cut(s) 304, 328, 507, 607, 770, 1026, 1206, 1423
Alw21I GWGCWC 3 cut(s) 486, 1028, 1155
Alw26I GTCTC 3 cut(s) 2, 199, 1444
AlwI GGATC 4 cut(s) 116, 785, 882, 1052
Aor51HI AGCGCT 1 cut(s) 952
AoxI GGCC 3 cut(s) 440, 491, 899
ArsI GACNNNNNNTTYG 2 cut(s) 1215, 1247
AseI ATTAAT 1 cut(s) 564
AsiGI ACCGGT 1 cut(s) 1401
Asp700I GAANNNNTTC 1 cut(s) 1266
Asp718I GGTACC 1 cut(s) 557
AspLEI GCGC 2 cut(s) 361, 953
AspS9I GGNCC 4 cut(s) 491, 626, 934, 1368
AsuHPI GGTGA 3 cut(s) 88, 257, 1438
AvaII GGWCC 3 cut(s) 626, 934, 1368
BanI GGYRCC 2 cut(s) 358, 557
BanII GRGCYC 1 cut(s) 1028
Bbv12I GWGCWC 3 cut(s) 486, 1028, 1155
BccI CCATC 6 cut(s) 149, 242, 776, 910, 1157, 1379
BceAI ACGGC 3 cut(s) 346, 372, 914
BcgI CGANNNNNNTGC 4 cut(s) 175, 209, 836, 870
BciT130I CCWGG 4 cut(s) 342, 489, 752, 1059
BciVI GTATCC 1 cut(s) 1259
BcoDI GTCTC 3 cut(s) 2, 199, 1444
BcuI ACTAGT 1 cut(s) 374
BfaI CTAG 1 cut(s) 375
BfmI CTRYAG 1 cut(s) 1090
BfoI RGCGCY 2 cut(s) 362, 954
BfuAI ACCTGC 1 cut(s) 1020
BfuI GTATCC 1 cut(s) 1259
BglI GCCNNNNNGGC 1 cut(s) 343
BisI GCNGC 2 cut(s) 114, 213
BlsI GCNGC 2 cut(s) 115, 214
Bme1390I CCNGG 4 cut(s) 342, 489, 752, 1059
Bme18I GGWCC 3 cut(s) 626, 934, 1368
BmgT120I GGNCC 4 cut(s) 491, 626, 934, 1368
BmiI GGNNCC 6 cut(s) 29, 62, 360, 559, 756, 1369
BmrFI CCNGG 4 cut(s) 342, 489, 752, 1059
BmsI GCATC 4 cut(s) 131, 394, 438, 1224
Bpu10I CCTNAGC 1 cut(s) 1193
BpuEI CTTGAG 1 cut(s) 1012
BsaHI GRCGYC 1 cut(s) 359
BsaI GGTCTC 2 cut(s) 2, 199
BsaWI WCCGGW 2 cut(s) 1186, 1401
BsaXI ACNNNNNCTCC 4 cut(s) 1221, 1251, 1424, 1454
Bsc4I CCNNNNNNNGG 9 cut(s) 24, 105, 198, 467, 500, 943, 1064, 1163, 1225
Bse118I RCCGGY 2 cut(s) 208, 1401
Bse1I ACTGG 1 cut(s) 1336
BseBI CCWGG 4 cut(s) 342, 489, 752, 1059
BseGI GGATG 3 cut(s) 433, 706, 1215
BseLI CCNNNNNNNGG 9 cut(s) 24, 105, 198, 467, 500, 943, 1064, 1163, 1225
BseMII CTCAG 2 cut(s) 194, 399
BseNI ACTGG 1 cut(s) 1336
BseYI CCCAGC 2 cut(s) 304, 870
Bsh1236I CGCG 2 cut(s) 622, 1236
Bsh1285I CGRYCG 1 cut(s) 1174
BshFI GGCC 3 cut(s) 442, 493, 901
BshNI GGYRCC 2 cut(s) 358, 557
BshTI ACCGGT 1 cut(s) 1401
BsiEI CGRYCG 1 cut(s) 1174
BsiHKAI GWGCWC 3 cut(s) 486, 1028, 1155
BsiSI CCGG 5 cut(s) 172, 182, 209, 1187, 1402
BslFI GGGAC 3 cut(s) 857, 1211, 1381
BslI CCNNNNNNNGG 9 cut(s) 24, 105, 198, 467, 500, 943, 1064, 1163, 1225
BsmAI GTCTC 3 cut(s) 2, 199, 1444
BsmFI GGGAC 3 cut(s) 857, 1211, 1381
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 3 cut(s) 442, 493, 901
Bso31I GGTCTC 2 cut(s) 2, 199
Bsp1286I GDGCHC 3 cut(s) 486, 1028, 1155
Bsp143I GATC 5 cut(s) 108, 790, 874, 1044, 1171
Bsp19I CCATGG 1 cut(s) 1252
BspACI CCGC 8 cut(s) 25, 55, 114, 212, 239, 346, 937, 1053
BspANI GGCC 3 cut(s) 442, 493, 901
BspCNI CTCAG 2 cut(s) 195, 400
BspFNI CGCG 2 cut(s) 622, 1236
BspLI GGNNCC 6 cut(s) 29, 62, 360, 559, 756, 1369
BspMI ACCTGC 1 cut(s) 1020
BspPI GGATC 4 cut(s) 116, 785, 882, 1052
BspT107I GGYRCC 2 cut(s) 358, 557
BspTNI GGTCTC 2 cut(s) 2, 199
BsrBI CCGCTC 1 cut(s) 939
BsrFI RCCGGY 2 cut(s) 208, 1401
BsrI ACTGG 1 cut(s) 1336
BssAI RCCGGY 2 cut(s) 208, 1401
BssMI GATC 5 cut(s) 108, 790, 874, 1044, 1171
BssNI GRCGYC 1 cut(s) 359
BssT1I CCWWGG 2 cut(s) 18, 1252
Bst2UI CCWGG 4 cut(s) 342, 489, 752, 1059
Bst4CI ACNGT 2 cut(s) 827, 1003
Bst6I CTCTTC 4 cut(s) 98, 472, 1057, 1257
BstACI GRCGYC 1 cut(s) 359
BstC8I GCNNGC 3 cut(s) 955, 1035, 1238
BstDEI CTNAG 3 cut(s) 203, 408, 1193
BstDSI CCRYGG 2 cut(s) 1219, 1252
BstENI CCTNNNNNAGG 1 cut(s) 1062
BstF5I GGATG 3 cut(s) 433, 706, 1215
BstFNI CGCG 2 cut(s) 622, 1236
BstH2I RGCGCY 2 cut(s) 362, 954
BstHHI GCGC 2 cut(s) 361, 953
BstKTI GATC 5 cut(s) 111, 793, 877, 1047, 1174
BstMAI GTCTC 3 cut(s) 2, 199, 1444
BstMBI GATC 5 cut(s) 108, 790, 874, 1044, 1171
BstMCI CGRYCG 1 cut(s) 1174
BstNI CCWGG 4 cut(s) 342, 489, 752, 1059
BstSCI CCNGG 4 cut(s) 340, 487, 750, 1057
BstSFI CTRYAG 1 cut(s) 1090
BstUI CGCG 2 cut(s) 622, 1236
BstX2I RGATCY 1 cut(s) 790
BstYI RGATCY 1 cut(s) 790
BsuI GTATCC 1 cut(s) 1259
BsuRI GGCC 3 cut(s) 442, 493, 901
BtgI CCRYGG 2 cut(s) 1219, 1252
BtsCI GGATG 3 cut(s) 433, 706, 1215
BtsI GCAGTG 2 cut(s) 455, 976
BtsIMutI CAGTG 4 cut(s) 455, 976, 1308, 1343
BveI ACCTGC 1 cut(s) 1020
Cac8I GCNNGC 3 cut(s) 955, 1035, 1238
CfoI GCGC 2 cut(s) 361, 953
Cfr10I RCCGGY 2 cut(s) 208, 1401
Cfr13I GGNCC 4 cut(s) 491, 626, 934, 1368
CseI GACGC 1 cut(s) 1067
Csp6I GTAC 2 cut(s) 558, 880
CspAI ACCGGT 1 cut(s) 1401
CspCI CAANNNNNGTGG 2 cut(s) 566, 601
CviAII CATG 6 cut(s) 193, 515, 582, 801, 1253, 1450
CviQI GTAC 2 cut(s) 558, 880
DdeI CTNAG 3 cut(s) 203, 408, 1193
DinI GGCGCC 1 cut(s) 360
DpnI GATC 5 cut(s) 110, 792, 876, 1046, 1173
DpnII GATC 5 cut(s) 108, 790, 874, 1044, 1171
EaeI YGGCCR 1 cut(s) 899
Eam1104I CTCTTC 4 cut(s) 98, 472, 1057, 1257
EarI CTCTTC 4 cut(s) 98, 472, 1057, 1257
Ecl136II GAGCTC 1 cut(s) 1026
Eco130I CCWWGG 2 cut(s) 18, 1252
Eco24I GRGCYC 1 cut(s) 1028
Eco31I GGTCTC 2 cut(s) 2, 199
Eco47I GGWCC 3 cut(s) 626, 934, 1368
Eco47III AGCGCT 1 cut(s) 952
Eco53kI GAGCTC 1 cut(s) 1026
Eco57I CTGAAG 2 cut(s) 71, 420
EcoICRI GAGCTC 1 cut(s) 1026
EcoNI CCTNNNNNAGG 1 cut(s) 1062
EcoRII CCWGG 4 cut(s) 340, 487, 750, 1057
EcoT14I CCWWGG 2 cut(s) 18, 1252
EcoT38I GRGCYC 1 cut(s) 1028
EgeI GGCGCC 1 cut(s) 360
EheI GGCGCC 1 cut(s) 360
ErhI CCWWGG 2 cut(s) 18, 1252
FaeI CATG 6 cut(s) 196, 518, 585, 804, 1256, 1453
FaqI GGGAC 3 cut(s) 857, 1211, 1381
FatI CATG 6 cut(s) 192, 514, 581, 800, 1252, 1449
FauNDI CATATG 3 cut(s) 381, 466, 712
Fnu4HI GCNGC 2 cut(s) 114, 213
FokI GGATG 3 cut(s) 440, 693, 1202
FriOI GRGCYC 1 cut(s) 1028
Fsp4HI GCNGC 2 cut(s) 114, 213
FspBI CTAG 1 cut(s) 375
GlaI GCGC 2 cut(s) 360, 952
GluI GCNGC 2 cut(s) 114, 213
GsaI CCCAGC 2 cut(s) 308, 874
HaeII RGCGCY 2 cut(s) 362, 954
HaeIII GGCC 3 cut(s) 442, 493, 901
HapII CCGG 5 cut(s) 172, 182, 209, 1187, 1402
HgaI GACGC 1 cut(s) 1067
HhaI GCGC 2 cut(s) 361, 953
Hin1I GRCGYC 1 cut(s) 359
Hin1II CATG 6 cut(s) 196, 518, 585, 804, 1256, 1453
Hin6I GCGC 2 cut(s) 359, 951
HinP1I GCGC 2 cut(s) 359, 951
HindIII AAGCTT 3 cut(s) 505, 605, 1204
HinfI GANTC 7 cut(s) 42, 385, 511, 686, 797, 1096, 1267
HpaII CCGG 5 cut(s) 172, 182, 209, 1187, 1402
HphI GGTGA 3 cut(s) 88, 257, 1438
Hpy166II GTNNAC 1 cut(s) 629
Hpy188I TCNGA 4 cut(s) 639, 1044, 1101, 1272
Hpy188III TCNNGA 6 cut(s) 106, 794, 1048, 1135, 1292, 1397
Hpy8I GTNNAC 1 cut(s) 629
Hpy99I CGWCG 1 cut(s) 851
HpyAV CCTTC 8 cut(s) 75, 79, 110, 127, 274, 307, 471, 1389
HpyCH4III ACNGT 2 cut(s) 827, 1003
HpyCH4IV ACGT 1 cut(s) 1103
HpyCH4V TGCA 3 cut(s) 424, 720, 857
HpyF3I CTNAG 3 cut(s) 203, 408, 1193
HpySE526I ACGT 1 cut(s) 1103
Hsp92I GRCGYC 1 cut(s) 359
Hsp92II CATG 6 cut(s) 196, 518, 585, 804, 1256, 1453
HspAI GCGC 2 cut(s) 359, 951
KasI GGCGCC 1 cut(s) 358
KpnI GGTACC 1 cut(s) 561
Kzo9I GATC 5 cut(s) 108, 790, 874, 1044, 1171
LmnI GCTCC 4 cut(s) 66, 760, 775, 1023
LweI GCATC 4 cut(s) 131, 394, 438, 1224
MaeI CTAG 1 cut(s) 375
MaeII ACGT 1 cut(s) 1103
MaeIII GTNAC 7 cut(s) 253, 742, 827, 923, 997, 1079, 1282
MalI GATC 5 cut(s) 110, 792, 876, 1046, 1173
MbiI CCGCTC 1 cut(s) 939
MboI GATC 5 cut(s) 108, 790, 874, 1044, 1171
MboII GAAGA 8 cut(s) 85, 322, 413, 489, 601, 1074, 1274, 1366
MflI RGATCY 1 cut(s) 790
MhlI GDGCHC 3 cut(s) 486, 1028, 1155
MluCI AATT 3 cut(s) 732, 852, 885
Mly113I GGCGCC 1 cut(s) 359
MlyI GAGTC 2 cut(s) 36, 379
MmeI TCCRAC 1 cut(s) 1102
MroXI GAANNNNTTC 1 cut(s) 1266
MseI TTAA 4 cut(s) 219, 366, 528, 564
MslI CAYNNNNRTG 4 cut(s) 711, 836, 1158, 1257
MspI CCGG 5 cut(s) 172, 182, 209, 1187, 1402
MspR9I CCNGG 4 cut(s) 342, 489, 752, 1059
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 4 cut(s) 342, 489, 752, 1059
MvnI CGCG 2 cut(s) 622, 1236
NarI GGCGCC 1 cut(s) 359
NcoI CCATGG 1 cut(s) 1252
NdeI CATATG 3 cut(s) 381, 466, 712
NdeII GATC 5 cut(s) 108, 790, 874, 1044, 1171
NlaIII CATG 6 cut(s) 196, 518, 585, 804, 1256, 1453
NlaIV GGNNCC 6 cut(s) 29, 62, 360, 559, 756, 1369
NmeAIII GCCGAG 1 cut(s) 222
NmuCI GTSAC 3 cut(s) 997, 1079, 1282
PasI CCCWGGG 1 cut(s) 488
PctI GAATGC 1 cut(s) 426
PdmI GAANNNNTTC 1 cut(s) 1266
PfeI GAWTC 5 cut(s) 511, 686, 797, 1096, 1267
PinAI ACCGGT 1 cut(s) 1401
PkrI GCNGC 2 cut(s) 115, 214
Ple19I CGATCG 1 cut(s) 1174
PleI GAGTC 2 cut(s) 36, 379
PluTI GGCGCC 1 cut(s) 362
PpsI GAGTC 2 cut(s) 36, 379
PshBI ATTAAT 1 cut(s) 564
PsiI TTATAA 1 cut(s) 542
Psp124BI GAGCTC 1 cut(s) 1028
Psp6I CCWGG 4 cut(s) 340, 487, 750, 1057
PspFI CCCAGC 2 cut(s) 304, 870
PspGI CCWGG 4 cut(s) 340, 487, 750, 1057
PspN4I GGNNCC 6 cut(s) 29, 62, 360, 559, 756, 1369
PspPI GGNCC 4 cut(s) 491, 626, 934, 1368
PsuI RGATCY 1 cut(s) 790
PvuI CGATCG 1 cut(s) 1174
RsaI GTAC 2 cut(s) 559, 881
RsaNI GTAC 2 cut(s) 558, 880
RseI CAYNNNNRTG 4 cut(s) 711, 836, 1158, 1257
SacI GAGCTC 1 cut(s) 1028
SaqAI TTAA 4 cut(s) 219, 366, 528, 564
SatI GCNGC 2 cut(s) 114, 213
Sau3AI GATC 5 cut(s) 108, 790, 874, 1044, 1171
Sau96I GGNCC 4 cut(s) 491, 626, 934, 1368
SchI GAGTC 2 cut(s) 36, 379
ScrFI CCNGG 4 cut(s) 342, 489, 752, 1059
SduI GDGCHC 3 cut(s) 486, 1028, 1155
SfaNI GCATC 4 cut(s) 131, 394, 438, 1224
SfcI CTRYAG 1 cut(s) 1090
SfoI GGCGCC 1 cut(s) 360
SinI GGWCC 3 cut(s) 626, 934, 1368
SmiMI CAYNNNNRTG 4 cut(s) 711, 836, 1158, 1257
SmlI CTYRAG 1 cut(s) 1027
SmoI CTYRAG 1 cut(s) 1027
SpeI ACTAGT 1 cut(s) 374
Sse9I AATT 3 cut(s) 732, 852, 885
SsiI CCGC 8 cut(s) 25, 55, 114, 212, 239, 346, 937, 1053
SspDI GGCGCC 1 cut(s) 358
SspI AATATT 1 cut(s) 727
SspMI CTAG 1 cut(s) 375
SstI GAGCTC 1 cut(s) 1028
StyD4I CCNGG 4 cut(s) 340, 487, 750, 1057
StyI CCWWGG 2 cut(s) 18, 1252
TaaI ACNGT 2 cut(s) 827, 1003
TaiI ACGT 1 cut(s) 1106
TaqI TCGA 4 cut(s) 45, 1117, 1174, 1291
TasI AATT 3 cut(s) 732, 852, 885
TauI GCSGC 2 cut(s) 116, 215
TfiI GAWTC 5 cut(s) 511, 686, 797, 1096, 1267
Tru1I TTAA 4 cut(s) 219, 366, 528, 564
Tru9I TTAA 4 cut(s) 219, 366, 528, 564
TscAI CASTG 4 cut(s) 462, 976, 1308, 1343
TseFI GTSAC 3 cut(s) 997, 1079, 1282
Tsp45I GTSAC 3 cut(s) 997, 1079, 1282
TspDTI ATGAA 4 cut(s) 453, 503, 695, 789
TspGWI ACGGA 6 cut(s) 242, 864, 897, 909, 1235, 1236
TspRI CASTG 4 cut(s) 462, 976, 1308, 1343
VpaK11BI GGWCC 3 cut(s) 626, 934, 1368
VspI ATTAAT 1 cut(s) 564
XagI CCTNNNNNAGG 1 cut(s) 1062
XmnI GAANNNNTTC 1 cut(s) 1266
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.