Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0007464

Physical Location & Seq

Genomic Coordinates Reverse (-)

22780916 .. 22786307

Gene Structure 5392 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1212600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGGAGAGGGTTTCAGAATCAAAGGTGCTTCCTAAGAAATATGATGTGGGACACGAGGTGATTTATTCATATTCGGCCTCAACGAGAGGAGTGGGTCACTTGTCAAACACACGTTCTGGTTTGGCGAGAGCAGCTGTTGCTTCTGCAAGAGACAGGGGTCAATTTTCCAATGGGCAATTGGGCTTGGTGAGAGAAGAAATTGCATTGGCAAGGCAAATGGCTGAATTGTCAAATGTGCATTCTGTTATGGTAAAAGATGGAAGATCTAATGCGCGCGAGTCTCAAGATTCAGACTCGCCTACACCTTTAAGAACAACAAAAGGAAAGATCTCTTTGCCAGAAGAGGAAGAACTTCTGTCTGACTCTCTCACATTCAAGGGAAGTATAGATTCATTTGATGAAGGGGCTCCTAGTTCTTTTGCCGGGGTTAGTCACCCTCCAGAACCTGTTGATATTGATTTAATGAAACCAGTGTATGTACCGATTGGTCAGAATAAGTCCGAGGCTGGATGCCTGATGAAGAGCCTATCTATGAAAGGCCCTTTTCTAGAAGATCTTTCCCTCCGTGTTCCTGCTAAGAAACCAAGTTCAACCGTTCTTTCCCCTGCGGAAAGCATGCTTGAAGAACCCAATGACATAGGTGCATTGTCTCCACCATTTGTGGTTCAGCGTGCGTCACAAAATACGGAAAGTTCACTCCTTCCTCCGGATTCTGAGGAGAAGGAGTGTGTTTGGGATGCTTCTTTGCCTCCAAGTGGCAATGTAAGTCCACTGAGTAGCATCGATAGTACTGGCGTTGTCACTGCTATGAGCATTGTCAATAGCTGTGGTAGTACATATAGGAGTGACGTAATCACTAGTGATGGCATGCTTAGTATCGAGAGAAACTGTGAAAGTGCCAAAGGGAGTGTTAGAAGGGAATCACTTGAGAGTCCAAAATCTAGCGTTAGTCGAGCAAGTGATAGCAGTGGCCTTAGTGATGACAGCAACTGGAGCAACCTTACTGGGAGTGCTAATAAGCCTCATAAAGGAAATGATCCTAGGTGGAAGGCTATTCTTGCTATTCGAGCTCGAGATGGGATTTTGGGTATGAGCCATTTTAGATTACTCAAACGGCTTGGTTGTGGTGATATTGGCAGTGTTTATCTTTCAGAACTAAGTGGAACCCGTTGTTATTTTGCAATGAAAGTAATGGACAAGGCATCCCTTGCAAGCAGGAAGAAGTTGACTAGGGCTCAGACAGAAAGGGAGATTTTGCAACTGCTGGACCATCCATTCCTTCCAACTTTATATACACATTTTGAGACCGACAGATTCTGCTGTTTGGTCATGGAATATTGTCCAGGTGGTGATCTGCACACTCTGAGGCAACGACAACCTGGAAAACATTTCTCAGAGTATGCTGCGAGATTCTATGCTGCGGAGGTTCTGTTGGCACTGGAGTATCTACACATGCTTGGAGTAGTCTACAGGGACTTAAAACCTGAAAATGTGCTTGTCCGTGATGATGGCCACATAATGCTCTCAGACTTCGATCTTTCACTGAGATGTGCTGTTTCTCCGACCCTGATAAGAACTTCATATGATTCGGATCCGTCAAAACGAGCAGCAGGTGGTGCATTCTGTGTCCAGCCTGCATGTATTGAGCCCTCATCGGTATGCATTCAGCCTGCTTGTTTTATCCCAAGGTTCTTCCCTCAGAAAAGCAAGAAGAGCCGAAAGCCTCGAGCTGAGCCTGGGTTTGCCACCAATGCACTTCCAGAGCTTGTTGCAGAGCCTACTCAAGCTCGGTCTATGTCTTTTGTTGGAACGCATGAATACCTAGCCCCTGAAATTATTAAGGGAGAAGGCCATGGTAGTGCAGTTGATTGGTGGACGTTTGGTATATTCTTGCATGAATTACTGTATGGCAAAACCCCTTTTAAAGGTTCAGGAAACCGTGCTACACTGTTCAATGTGGTGGGGCAGCAACTTAGATTCCCAGATTCCCCCGCAACTAGTTATGCAAGCCGCGATCTAATTCGGGGGTTGCTGGTGAAAGAGCCACAGCACCGGCTTGGGGTGAAAAGGGGTGCAACTGAGATCAAACAGCATCCCTTCTTCGAAGGTGTAAATTGGGCTTTAATACGATGCAGCACACCACCAGAAGTACCAAGACCGATGGAAGCTGAGCTGCCAGTGAAATTTGGGGCAGTTGAGGCAGTTGGTGTCGGAAGCAACAGTAAAAGAATGGCTGGGGCAGACGTGAAGTCCGGGGGTAAATATCTAGACTTTGAGTTCTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

762

Amino Acids

83.16

Weight (kDa)

7.24

Isoelectric Point (pI)

50.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	368 - 517	3.2e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	368 - 516	3e-18	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	598 - 701	1.2e-19	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	605 - 687	3.7e-06	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007464)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44830 AT2G44830 AT2G44830
fragaria_vesca	FvH4_7g10180 FvH4_7g10180 FvH4_7g10180 FvH4_7g10180 FvH4_7g10180
malus_domestica	MD02G1212600.v1.1 MD07G1108800.v1.1
prunus_persica	Prupe.2G131700_v2.0.a1 Prupe.2G131700_v2.0.a1
pyrus_communis	pycom02g17610 pycom07g09340
rosa_chinensis	RchiOBHm_Chr1g0347901
rosa_laevigata	RLG00000028705
rosa_multiflora	Rmu_sc0034852.1_g000001 Rmu_ssc0000342.1_g000010
rosa_roxburghii	Rroxscaffold_4G00307390
rosa_rugosa	Rorug01G0190400
rosa_samantha	Rh1AG208600 Rh1BG173200 Rh1CG192900 Rh1DG203100
rosa_wichuraiana	Rw1G017340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1601
Acc36I	ACCTGC	1 cut(s)	1601
AccI	GTMKAC	1 cut(s)	1467
AccII	CGCG	3 cut(s)	274, 276, 2013
AccIII	TCCGGA	1 cut(s)	706
AciI	CCGC	4 cut(s)	608, 1421, 1992, 2011
AclWI	GGATC	3 cut(s)	1031, 1586, 1599
AcoI	YGGCCR	1 cut(s)	1510
AcsI	RAATTY	1 cut(s)	2183
AdeI	CACNNNGTG	1 cut(s)	58
AfaI	GTAC	4 cut(s)	480, 790, 835, 2151
AfiI	CCNNNNNNNGG	5 cut(s)	706, 755, 1028, 1925, 2059
AflIII	ACRYGT	1 cut(s)	110
AgsI	TTSAA	4 cut(s)	376, 591, 623, 1954
AhdI	GACNNNNNGTC	1 cut(s)	2248
AhlI	ACTAGT	2 cut(s)	857, 1997
AjiI	CACGTC	1 cut(s)	2245
AjnI	CCWGG	3 cut(s)	1342, 1378, 1735
AluBI	AGCT	8 cut(s)	134, 825, 1070, 1730, 1765, 1787, 2168, 2173
AluI	AGCT	8 cut(s)	134, 825, 1070, 1730, 1765, 1787, 2168, 2173
Alw21I	GWGCWC	1 cut(s)	1072
Alw26I	GTCTC	4 cut(s)	144, 285, 654, 1298
AlwI	GGATC	3 cut(s)	1031, 1586, 1599
AlwNI	CAGNNNCTG	3 cut(s)	446, 990, 1317
Ama87I	CYCGRG	2 cut(s)	1071, 1725
Aor13HI	TCCGGA	1 cut(s)	706
AoxI	GGCC	5 cut(s)	75, 538, 970, 1510, 1849
ApeKI	GCWGC	7 cut(s)	131, 1403, 1418, 1607, 1966, 2133, 2173
ApoI	RAATTY	1 cut(s)	2183
Asp700I	GAANNNNTTC	1 cut(s)	351
AspA2I	CCTAGG	1 cut(s)	1040
AspLEI	GCGC	2 cut(s)	274, 276
AspS9I	GGNCC	2 cut(s)	539, 1267
AsuC2I	CCSGG	2 cut(s)	424, 2254
AsuHPI	GGTGA	7 cut(s)	70, 199, 425, 1139, 1361, 2047, 2074
AsuII	TTCGAA	1 cut(s)	2103
AvaI	CYCGRG	2 cut(s)	1071, 1725
AvaII	GGWCC	1 cut(s)	1267
AvrII	CCTAGG	1 cut(s)	1040
BaeI	ACNNNNGTAYC	2 cut(s)	1427, 1460
BalI	TGGCCA	1 cut(s)	1512
BamHI	GGATCC	1 cut(s)	1591
BanII	GRGCYC	4 cut(s)	409, 1072, 1237, 1650
BauI	CACGAG	1 cut(s)	53
Bbv12I	GWGCWC	1 cut(s)	1072
BbvI	GCAGC	7 cut(s)	143, 1390, 1405, 1619, 1978, 2145, 2160
BccI	CCATC	6 cut(s)	251, 857, 1070, 1278, 1502, 2155
BceAI	ACGGC	1 cut(s)	1130
BciT130I	CCWGG	3 cut(s)	1344, 1380, 1737
BcnI	CCSGG	2 cut(s)	424, 2254
BcoDI	GTCTC	4 cut(s)	144, 285, 654, 1298
BcuI	ACTAGT	2 cut(s)	857, 1997
BfaI	CTAG	9 cut(s)	411, 548, 858, 942, 1041, 1230, 1823, 1998, 2267
BfmI	CTRYAG	1 cut(s)	1468
BfuAI	ACCTGC	1 cut(s)	1601
BglII	AGATCT	3 cut(s)	263, 327, 553
BisI	GCNGC	8 cut(s)	132, 1404, 1419, 1608, 1967, 2011, 2134, 2174
BlnI	CCTAGG	1 cut(s)	1040
BlpI	GCTNAGC	2 cut(s)	1731, 2169
BlsI	GCNGC	8 cut(s)	133, 1405, 1420, 1609, 1968, 2012, 2135, 2175
BmcAI	AGTACT	1 cut(s)	790
Bme1390I	CCNGG	5 cut(s)	424, 1344, 1380, 1737, 2254
Bme18I	GGWCC	1 cut(s)	1267
BmeRI	GACNNNNNGTC	1 cut(s)	2248
BmeT110I	CYCGRG	2 cut(s)	1071, 1725
BmgBI	CACGTC	1 cut(s)	2245
BmgT120I	GGNCC	2 cut(s)	539, 1267
BmiI	GGNNCC	3 cut(s)	408, 1165, 1593
BmrFI	CCNGG	5 cut(s)	424, 1344, 1380, 1737, 2254
BmrI	ACTGGG	1 cut(s)	1014
BmsI	GCATC	6 cut(s)	500, 727, 789, 1211, 2101, 2120
BmuI	ACTGGG	1 cut(s)	1014
BoxI	GACNNNNGTC	1 cut(s)	156
BpmI	CTGGAG	3 cut(s)	423, 1012, 1460
Bpu1102I	GCTNAGC	2 cut(s)	1731, 2169
Bpu14I	TTCGAA	1 cut(s)	2103
BpuEI	CTTGAG	3 cut(s)	267, 947, 1767
BpuMI	CCSGG	2 cut(s)	424, 2254
Bsa29I	ATCGAT	1 cut(s)	783
BsaBI	GATNNNNATC	1 cut(s)	1590
BsaI	GGTCTC	1 cut(s)	1298
BsaJI	CCNNGG	7 cut(s)	423, 501, 1040, 1685, 1736, 1852, 2253
BsaWI	WCCGGW	1 cut(s)	706
BsaXI	ACNNNNNCTCC	4 cut(s)	710, 740, 1415, 1445
Bsc4I	CCNNNNNNNGG	5 cut(s)	706, 755, 1028, 1925, 2059
Bse118I	RCCGGY	1 cut(s)	2052
Bse1I	ACTGG	6 cut(s)	470, 796, 995, 1009, 1443, 2177
Bse3DI	GCAATG	2 cut(s)	766, 1188
Bse8I	GATNNNNATC	1 cut(s)	1590
BseAI	TCCGGA	1 cut(s)	706
BseBI	CCWGG	3 cut(s)	1344, 1380, 1737
BseCI	ATCGAT	1 cut(s)	783
BseDI	CCNNGG	7 cut(s)	423, 501, 1040, 1685, 1736, 1852, 2253
BseGI	GGATG	5 cut(s)	515, 742, 1202, 1270, 2092
BseJI	GATNNNNATC	1 cut(s)	1590
BseLI	CCNNNNNNNGG	5 cut(s)	706, 755, 1028, 1925, 2059
BseMI	GCAATG	2 cut(s)	766, 1188
BseNI	ACTGG	6 cut(s)	470, 796, 995, 1009, 1443, 2177
BsePI	GCGCGC	1 cut(s)	272
BseRI	GAGGAG	2 cut(s)	102, 731
BseXI	GCAGC	7 cut(s)	143, 1390, 1405, 1619, 1978, 2145, 2160
BseYI	CCCAGC	1 cut(s)	2234
BsgI	GTGCAG	2 cut(s)	1340, 1881
Bsh1236I	CGCG	3 cut(s)	274, 276, 2013
BshFI	GGCC	5 cut(s)	77, 540, 972, 1512, 1851
BshVI	ATCGAT	1 cut(s)	783
BsiHKAI	GWGCWC	1 cut(s)	1072
BsiHKCI	CYCGRG	2 cut(s)	1071, 1725
BsiSI	CCGG	4 cut(s)	423, 707, 2053, 2253
BslFI	GGGAC	2 cut(s)	63, 1487
BslI	CCNNNNNNNGG	5 cut(s)	706, 755, 1028, 1925, 2059
BsmAI	GTCTC	4 cut(s)	144, 285, 654, 1298
BsmFI	GGGAC	2 cut(s)	63, 1487
BsmI	GAATGC	3 cut(s)	238, 1619, 1662
BsnI	GGCC	5 cut(s)	77, 540, 972, 1512, 1851
Bso31I	GGTCTC	1 cut(s)	1298
BsoBI	CYCGRG	2 cut(s)	1071, 1725
Bsp119I	TTCGAA	1 cut(s)	2103
Bsp1286I	GDGCHC	4 cut(s)	409, 1072, 1237, 1650
Bsp13I	TCCGGA	1 cut(s)	706
Bsp143I	GATC	9 cut(s)	263, 327, 553, 1036, 1351, 1534, 1591, 2014, 2082
Bsp1720I	GCTNAGC	2 cut(s)	1731, 2169
Bsp19I	CCATGG	1 cut(s)	1852
BspACI	CCGC	4 cut(s)	608, 1421, 1992, 2011
BspANI	GGCC	5 cut(s)	77, 540, 972, 1512, 1851
BspDI	ATCGAT	1 cut(s)	783
BspEI	TCCGGA	1 cut(s)	706
BspFNI	CGCG	3 cut(s)	274, 276, 2013
BspLI	GGNNCC	3 cut(s)	408, 1165, 1593
BspMI	ACCTGC	1 cut(s)	1601
BspPI	GGATC	3 cut(s)	1031, 1586, 1599
BspQI	GCTCTTC	2 cut(s)	515, 1706
BspT104I	TTCGAA	1 cut(s)	2103
BspTNI	GGTCTC	1 cut(s)	1298
BsrDI	GCAATG	2 cut(s)	766, 1188
BsrFI	RCCGGY	1 cut(s)	2052
BsrI	ACTGG	6 cut(s)	470, 796, 995, 1009, 1443, 2177
BssAI	RCCGGY	1 cut(s)	2052
BssECI	CCNNGG	7 cut(s)	423, 501, 1040, 1685, 1736, 1852, 2253
BssHII	GCGCGC	1 cut(s)	272
BssMI	GATC	9 cut(s)	263, 327, 553, 1036, 1351, 1534, 1591, 2014, 2082
BssSI	CACGAG	1 cut(s)	53
BssT1I	CCWWGG	3 cut(s)	1040, 1685, 1852
Bst2BI	CACGAG	1 cut(s)	53
Bst2UI	CCWGG	3 cut(s)	1344, 1380, 1737
Bst4CI	ACNGT	6 cut(s)	595, 890, 1905, 1940, 1950, 2222
Bst6I	CTCTTC	3 cut(s)	336, 515, 1706
BstAPI	GCANNNNNTGC	3 cut(s)	137, 1208, 1616
BstBI	TTCGAA	1 cut(s)	2103
BstC8I	GCNNGC	8 cut(s)	274, 617, 672, 869, 1213, 1635, 1671, 2008
BstDSI	CCRYGG	1 cut(s)	1852
BstENI	CCTNNNNNAGG	2 cut(s)	1026, 1923
BstF5I	GGATG	5 cut(s)	515, 742, 1202, 1270, 2092
BstFNI	CGCG	3 cut(s)	274, 276, 2013
BstHHI	GCGC	2 cut(s)	274, 276
BstKTI	GATC	9 cut(s)	266, 330, 556, 1039, 1354, 1537, 1594, 2017, 2085
BstMAI	GTCTC	4 cut(s)	144, 285, 654, 1298
BstMBI	GATC	9 cut(s)	263, 327, 553, 1036, 1351, 1534, 1591, 2014, 2082
BstNI	CCWGG	3 cut(s)	1344, 1380, 1737
BstNSI	RCATGY	4 cut(s)	619, 871, 1456, 1641
BstPAI	GACNNNNGTC	1 cut(s)	156
BstSCI	CCNGG	5 cut(s)	422, 1342, 1378, 1735, 2252
BstSFI	CTRYAG	1 cut(s)	1468
BstUI	CGCG	3 cut(s)	274, 276, 2013
BstV1I	GCAGC	7 cut(s)	143, 1390, 1405, 1619, 1978, 2145, 2160
BstX2I	RGATCY	4 cut(s)	263, 327, 553, 1591
BstYI	RGATCY	4 cut(s)	263, 327, 553, 1591
Bsu15I	ATCGAT	1 cut(s)	783
BsuRI	GGCC	5 cut(s)	77, 540, 972, 1512, 1851
BsuTUI	ATCGAT	1 cut(s)	783
BtgI	CCRYGG	1 cut(s)	1852
BtrI	CACGTC	1 cut(s)	2245
BtsCI	GGATG	5 cut(s)	515, 742, 1202, 1270, 2092
BtsI	GCAGTG	3 cut(s)	801, 973, 1144
BtsIMutI	CAGTG	9 cut(s)	477, 770, 801, 973, 1144, 1436, 1541, 1946, 2184
BveI	ACCTGC	1 cut(s)	1601
Cac8I	GCNNGC	8 cut(s)	274, 617, 672, 869, 1213, 1635, 1671, 2008
CaiI	CAGNNNCTG	3 cut(s)	446, 990, 1317
CfoI	GCGC	2 cut(s)	274, 276
Cfr10I	RCCGGY	1 cut(s)	2052
Cfr13I	GGNCC	2 cut(s)	539, 1267
ClaI	ATCGAT	1 cut(s)	783
CseI	GACGC	1 cut(s)	663
Csp6I	GTAC	4 cut(s)	479, 789, 834, 2150
CspCI	CAANNNNNGTGG	2 cut(s)	808, 843
CviAII	CATG	8 cut(s)	616, 868, 1330, 1453, 1638, 1814, 1853, 1895
CviQI	GTAC	4 cut(s)	479, 789, 834, 2150
DpnI	GATC	9 cut(s)	265, 329, 555, 1038, 1353, 1536, 1593, 2016, 2084
DpnII	GATC	9 cut(s)	263, 327, 553, 1036, 1351, 1534, 1591, 2014, 2082
DraI	TTTAAA	1 cut(s)	1924
DraIII	CACNNNGTG	1 cut(s)	58
DriI	GACNNNNNGTC	1 cut(s)	2248
EaeI	YGGCCR	1 cut(s)	1510
Eam1104I	CTCTTC	3 cut(s)	336, 515, 1706
Eam1105I	GACNNNNNGTC	1 cut(s)	2248
EarI	CTCTTC	3 cut(s)	336, 515, 1706
Ecl136II	GAGCTC	1 cut(s)	1070
Eco130I	CCWWGG	3 cut(s)	1040, 1685, 1852
Eco24I	GRGCYC	4 cut(s)	409, 1072, 1237, 1650
Eco31I	GGTCTC	1 cut(s)	1298
Eco47I	GGWCC	1 cut(s)	1267
Eco53kI	GAGCTC	1 cut(s)	1070
Eco88I	CYCGRG	2 cut(s)	1071, 1725
EcoICRI	GAGCTC	1 cut(s)	1070
EcoNI	CCTNNNNNAGG	2 cut(s)	1026, 1923
EcoO109I	RGGNCCY	1 cut(s)	539
EcoRII	CCWGG	3 cut(s)	1342, 1378, 1735
EcoT14I	CCWWGG	3 cut(s)	1040, 1685, 1852
EcoT22I	ATGCAT	1 cut(s)	1664
EcoT38I	GRGCYC	4 cut(s)	409, 1072, 1237, 1650
ErhI	CCWWGG	3 cut(s)	1040, 1685, 1852
FaeI	CATG	8 cut(s)	619, 871, 1333, 1456, 1641, 1817, 1856, 1898
FaqI	GGGAC	2 cut(s)	63, 1487
FatI	CATG	8 cut(s)	615, 867, 1329, 1452, 1637, 1813, 1852, 1894
FauI	CCCGC	1 cut(s)	1999
FauNDI	CATATG	1 cut(s)	1582
FblI	GTMKAC	1 cut(s)	1467
Fnu4HI	GCNGC	8 cut(s)	132, 1404, 1419, 1608, 1967, 2011, 2134, 2174
FokI	GGATG	5 cut(s)	522, 749, 1189, 1257, 2079
FriOI	GRGCYC	4 cut(s)	409, 1072, 1237, 1650
Fsp4HI	GCNGC	8 cut(s)	132, 1404, 1419, 1608, 1967, 2011, 2134, 2174
FspBI	CTAG	9 cut(s)	411, 548, 858, 942, 1041, 1230, 1823, 1998, 2267
GlaI	GCGC	2 cut(s)	273, 275
GluI	GCNGC	8 cut(s)	132, 1404, 1419, 1608, 1967, 2011, 2134, 2174
GsaI	CCCAGC	1 cut(s)	2238
GsuI	CTGGAG	3 cut(s)	423, 1012, 1460
HaeIII	GGCC	5 cut(s)	77, 540, 972, 1512, 1851
HapII	CCGG	4 cut(s)	423, 707, 2053, 2253
HgaI	GACGC	1 cut(s)	663
HhaI	GCGC	2 cut(s)	274, 276
Hin1II	CATG	8 cut(s)	619, 871, 1333, 1456, 1641, 1817, 1856, 1898
Hin6I	GCGC	2 cut(s)	272, 274
HinP1I	GCGC	2 cut(s)	272, 274
HincII	GTYRAC	1 cut(s)	1227
HindII	GTYRAC	1 cut(s)	1227
HpaII	CCGG	4 cut(s)	423, 707, 2053, 2253
HphI	GGTGA	7 cut(s)	70, 199, 425, 1139, 1361, 2047, 2074
Hpy166II	GTNNAC	5 cut(s)	695, 770, 1227, 1468, 1875
Hpy188III	TCNNGA	9 cut(s)	284, 440, 548, 707, 880, 1073, 1760, 1932, 2267
Hpy8I	GTNNAC	5 cut(s)	695, 770, 1227, 1468, 1875
HpyAV	CCTTC	9 cut(s)	395, 710, 715, 909, 1042, 1289, 1841, 2099, 2107
HpyCH4III	ACNGT	6 cut(s)	595, 890, 1905, 1940, 1950, 2222
HpyCH4IV	ACGT	4 cut(s)	112, 849, 1877, 2244
HpySE526I	ACGT	4 cut(s)	112, 849, 1877, 2244
Hsp92II	CATG	8 cut(s)	619, 871, 1333, 1456, 1641, 1817, 1856, 1898
HspAI	GCGC	2 cut(s)	272, 274
Kpn2I	TCCGGA	1 cut(s)	706
Kzo9I	GATC	9 cut(s)	263, 327, 553, 1036, 1351, 1534, 1591, 2014, 2082
LguI	GCTCTTC	2 cut(s)	515, 1706
LmnI	GCTCC	2 cut(s)	412, 993
Lsp1109I	GCAGC	7 cut(s)	143, 1390, 1405, 1619, 1978, 2145, 2160
LweI	GCATC	6 cut(s)	500, 727, 789, 1211, 2101, 2120
MaeI	CTAG	9 cut(s)	411, 548, 858, 942, 1041, 1230, 1823, 1998, 2267
MaeII	ACGT	4 cut(s)	112, 849, 1877, 2244
MaeIII	GTNAC	5 cut(s)	95, 431, 675, 799, 845
MalI	GATC	9 cut(s)	265, 329, 555, 1038, 1353, 1536, 1593, 2016, 2084
MboI	GATC	9 cut(s)	263, 327, 553, 1036, 1351, 1534, 1591, 2014, 2082
MfeI	CAATTG	1 cut(s)	176
MflI	RGATCY	4 cut(s)	263, 327, 553, 1591
MhlI	GDGCHC	4 cut(s)	409, 1072, 1237, 1650
MlsI	TGGCCA	1 cut(s)	1512
MluCI	AATT	9 cut(s)	161, 176, 198, 224, 1833, 1898, 2019, 2113, 2183
MluNI	TGGCCA	1 cut(s)	1512
MlyI	GAGTC	4 cut(s)	287, 287, 356, 940
MmeI	TCCRAC	4 cut(s)	1307, 1586, 1786, 2191
Mox20I	TGGCCA	1 cut(s)	1512
Mph1103I	ATGCAT	1 cut(s)	1664
MroI	TCCGGA	1 cut(s)	706
MroXI	GAANNNNTTC	1 cut(s)	351
MscI	TGGCCA	1 cut(s)	1512
MseI	TTAA	6 cut(s)	308, 461, 1478, 1839, 1923, 2123
MslI	CAYNNNNRTG	4 cut(s)	806, 1546, 1657, 1857
Msp20I	TGGCCA	1 cut(s)	1512
MspA1I	CMGCKG	1 cut(s)	134
MspI	CCGG	4 cut(s)	423, 707, 2053, 2253
MspR9I	CCNGG	5 cut(s)	424, 1344, 1380, 1737, 2254
MunI	CAATTG	1 cut(s)	176
Mva1269I	GAATGC	3 cut(s)	238, 1619, 1662
MvaI	CCWGG	3 cut(s)	1344, 1380, 1737
MvnI	CGCG	3 cut(s)	274, 276, 2013
NciI	CCSGG	2 cut(s)	424, 2254
NcoI	CCATGG	1 cut(s)	1852
NdeI	CATATG	1 cut(s)	1582
NdeII	GATC	9 cut(s)	263, 327, 553, 1036, 1351, 1534, 1591, 2014, 2082
NlaIII	CATG	8 cut(s)	619, 871, 1333, 1456, 1641, 1817, 1856, 1898
NlaIV	GGNNCC	3 cut(s)	408, 1165, 1593
NmuCI	GTSAC	5 cut(s)	95, 431, 675, 799, 845
NsiI	ATGCAT	1 cut(s)	1664
NspI	RCATGY	4 cut(s)	619, 871, 1456, 1641
NspV	TTCGAA	1 cut(s)	2103
PaeI	GCATGC	2 cut(s)	619, 871
PaeR7I	CTCGAG	2 cut(s)	1071, 1725
PaqCI	CACCTGC	1 cut(s)	1601
PauI	GCGCGC	1 cut(s)	272
PciSI	GCTCTTC	2 cut(s)	515, 1706
PcsI	WCGNNNNNNNCGW	1 cut(s)	80
PctI	GAATGC	3 cut(s)	238, 1619, 1662
PdmI	GAANNNNTTC	1 cut(s)	351
PkrI	GCNGC	8 cut(s)	133, 1405, 1420, 1609, 1968, 2012, 2135, 2175
PleI	GAGTC	4 cut(s)	286, 287, 356, 939
PpsI	GAGTC	4 cut(s)	286, 287, 356, 939
PshAI	GACNNNNGTC	1 cut(s)	156
Psp124BI	GAGCTC	1 cut(s)	1072
Psp6I	CCWGG	3 cut(s)	1342, 1378, 1735
PspFI	CCCAGC	1 cut(s)	2234
PspGI	CCWGG	3 cut(s)	1342, 1378, 1735
PspN4I	GGNNCC	3 cut(s)	408, 1165, 1593
PspPI	GGNCC	2 cut(s)	539, 1267
PspXI	VCTCGAGB	1 cut(s)	1725
PstNI	CAGNNNCTG	3 cut(s)	446, 990, 1317
PsuI	RGATCY	4 cut(s)	263, 327, 553, 1591
PteI	GCGCGC	1 cut(s)	272
PvuII	CAGCTG	1 cut(s)	134
RsaI	GTAC	4 cut(s)	480, 790, 835, 2151
RsaNI	GTAC	4 cut(s)	479, 789, 834, 2150
RseI	CAYNNNNRTG	4 cut(s)	806, 1546, 1657, 1857
SacI	GAGCTC	1 cut(s)	1072
SapI	GCTCTTC	2 cut(s)	515, 1706
SaqAI	TTAA	6 cut(s)	308, 461, 1478, 1839, 1923, 2123
SatI	GCNGC	8 cut(s)	132, 1404, 1419, 1608, 1967, 2011, 2134, 2174
Sau3AI	GATC	9 cut(s)	263, 327, 553, 1036, 1351, 1534, 1591, 2014, 2082
Sau96I	GGNCC	2 cut(s)	539, 1267
ScaI	AGTACT	1 cut(s)	790
SchI	GAGTC	4 cut(s)	287, 287, 356, 940
ScrFI	CCNGG	5 cut(s)	424, 1344, 1380, 1737, 2254
SduI	GDGCHC	4 cut(s)	409, 1072, 1237, 1650
SfaNI	GCATC	6 cut(s)	500, 727, 789, 1211, 2101, 2120
SfcI	CTRYAG	1 cut(s)	1468
Sfr274I	CTCGAG	2 cut(s)	1071, 1725
SfuI	TTCGAA	1 cut(s)	2103
SinI	GGWCC	1 cut(s)	1267
SlaI	CTCGAG	2 cut(s)	1071, 1725
SmiMI	CAYNNNNRTG	4 cut(s)	806, 1546, 1657, 1857
SmlI	CTYRAG	5 cut(s)	282, 926, 1071, 1725, 1782
SmoI	CTYRAG	5 cut(s)	282, 926, 1071, 1725, 1782
SpeI	ACTAGT	2 cut(s)	857, 1997
SphI	GCATGC	2 cut(s)	619, 871
Sse9I	AATT	9 cut(s)	161, 176, 198, 224, 1833, 1898, 2019, 2113, 2183
SsiI	CCGC	4 cut(s)	608, 1421, 1992, 2011
SspI	AATATT	1 cut(s)	1337
SspMI	CTAG	9 cut(s)	411, 548, 858, 942, 1041, 1230, 1823, 1998, 2267
SstI	GAGCTC	1 cut(s)	1072
StyD4I	CCNGG	5 cut(s)	422, 1342, 1378, 1735, 2252
StyI	CCWWGG	3 cut(s)	1040, 1685, 1852
TaaI	ACNGT	6 cut(s)	595, 890, 1905, 1940, 1950, 2222
TaiI	ACGT	4 cut(s)	115, 852, 1880, 2247
TaqI	TCGA	8 cut(s)	783, 879, 952, 1066, 1072, 1533, 1726, 2103
TaqII	GACCGA	3 cut(s)	1322, 1779, 2173
TasI	AATT	9 cut(s)	161, 176, 198, 224, 1833, 1898, 2019, 2113, 2183
TatI	WGTACW	2 cut(s)	788, 833
TauI	GCSGC	1 cut(s)	2013
Tru1I	TTAA	6 cut(s)	308, 461, 1478, 1839, 1923, 2123
Tru9I	TTAA	6 cut(s)	308, 461, 1478, 1839, 1923, 2123
TscAI	CASTG	9 cut(s)	477, 777, 808, 973, 1144, 1443, 1548, 1953, 2184
TseFI	GTSAC	5 cut(s)	95, 431, 675, 799, 845
TseI	GCWGC	7 cut(s)	131, 1403, 1418, 1607, 1966, 2133, 2173
Tsp45I	GTSAC	5 cut(s)	95, 431, 675, 799, 845
TspGWI	ACGGA	4 cut(s)	554, 701, 1490, 1584
TspRI	CASTG	9 cut(s)	477, 777, 808, 973, 1144, 1443, 1548, 1953, 2184
VpaK11BI	GGWCC	1 cut(s)	1267
XagI	CCTNNNNNAGG	2 cut(s)	1026, 1923
XapI	RAATTY	1 cut(s)	2183
XbaI	TCTAGA	2 cut(s)	547, 2266
XceI	RCATGY	4 cut(s)	619, 871, 1456, 1641
XcmI	CCANNNNNNNNNTGG	1 cut(s)	175
XhoI	CTCGAG	2 cut(s)	1071, 1725
XmaJI	CCTAGG	1 cut(s)	1040
XmiI	GTMKAC	1 cut(s)	1467
XmnI	GAANNNNTTC	1 cut(s)	351
XspI	CTAG	9 cut(s)	411, 548, 858, 942, 1041, 1230, 1823, 1998, 2267
ZrmI	AGTACT	1 cut(s)	790
Zsp2I	ATGCAT	1 cut(s)	1664

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.