Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0015427

Physical Location & Seq

Genomic Coordinates Forward (+)

29800774 .. 29802318

Gene Structure 1545 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1247800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1545 bp

ATGGCTACTTTCTCCTTCTCCTCTGCCTCTCTTTCCTCGTACTACCACCTTTTTTTCACCCTCCTTGCATTCCTCGTTTCTGGTTTAATCTTCCTGCTCACCCACAAGTCCAAATCCAAGCGCCCCAATCTCCCTCCCGGTCCGCCGGGATGGCCGATTGTCGGCAACCTCTTCCAGTTTGCCCGCTCCGGAAAACCATTCTTCCAGTACGCCGATGATCTCCGGCAAAAGTACGGCCCCATTTTCACTCTCAAAATGGGTACCCGAACCATGATCATCCTGAGCGACGCCAAGCTCGTCCACGAAGCGTTGATCGAGAAGGGCGCGGTGTTCGCCACCCGACCCAGAGAGAACCCGACCCGAACAATCTTCAGCTGCAACAAGTTCACCGTCAACGCCGCTGTCTACGGCCCGGTGTGGCGGTCCCTGCGGCGGAACATGGTCCAGAACATGCTCAGCTCCACTCGCTTGAAGGAGTTCCGAAGTGTCCGAGAAAATGCCATGGACACACTCATCGAGCGGATTAAGGCCGACGCCAAGGCCAATGACGGCGTCGTTTCGGTCCTCAAAAACGCGCGCTTCGCCGTGTTTTGCATCCTTTTGGCAATGTGTTTCGGGATCGAGATGGACGAGGAGACCGTGGAGAAAATGGATCAGATAATGAAAACGGTTTTAATGGTGCTCGACCCGAGAATGGACGATTATTTGCCGATTTTAAGCCCCTTTTTCTCCAAGCAGAGGAAGAGAGCTCTGGAAGTGAGAAACAAGCAGATTCAGTTCATTGTCCCGTTCATCGAACGACGTCGCAGGGCGCTCAAGAACCCAGGGTCGGATGTCAAAGCGACGTCATTCTCCTACCTCGACACTCTTTTCGATCTCAAAGTGGAGGGACAAAAATCTTCGCCTTCTCATGCTGAATTGGTCACGCTTTGTTCGGAGTTTCTGAATGGCGGCACTGACACGACGGCGACGGCTCTCGAATGGGGAATTGCTCAGCTCATAGCAAATCCGGACATTCAAGAGAAGCTGTACAGCGAAATTAAAGCGAGTGTGGGCGACAGAAAAGTTGACGAGAAAGATGTGGAGAAAATGCCTTACTTGCAAGCTGTGGTGAAGGAACTTTTACGTAAACACCCTCCCACCTACTTTTCACTAACCCATGCAGTAACTGAGCCTACTACTTTGGCCGGGTACGACATACCGACGGATGTAAATGTTGAGATTTACTTGCCCGGCATATCGGACGACTCCAAATTGTGGTCCAACCCAGAAAAGTTCGATCCCGACCGGTTTGTGTCCGGTAGGGAGGAGGCGGATATAACCGGGGTGACCGGAATTAAAATGATGCCGTTCGGGGTCGGCAGGAGGATATGCCCGGGGTTGGGCATGGCGACCGTGCATGTTCATCTAATGCTGGCTAGAATGGTGCAGGAGTTCGAGTGGAGTGCTTACCAGGCTAGTGAGAAGATAGATTTTGCTGGAAAATTGGAGTTCACTGTGGTGATGAAGAACACTCTTAGAGCTATGATCAAGCCTAGAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0006082 GO:0006629 GO:0006631 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010143 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019395 GO:0019748 GO:0019752 GO:0030258 GO:0032787 GO:0034440 GO:0043170 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044550 GO:0052722 GO:0055114 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00073 map00073

Pfam Domains

Protein Families

p450

Protein Analysis

515

Amino Acids

58.11

Weight (kDa)

9.21

Isoelectric Point (pI)

40.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	45 - 499	1.8e-116	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0015427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10570 AT5G04660
fragaria_vesca	FvH4_7g05960
malus_domestica	MD02G1247800.v1.1 MD07G1068700.v1.1
prunus_persica	Prupe.2G086300_v2.0.a1
pyrus_communis	pycom02g21180 pycom07g05280
rosa_chinensis	RchiOBHm_Chr1g0333181
rosa_laevigata	RLG00000029620
rosa_multiflora	Rmu_sc0001214.1_g000041
rosa_roxburghii	Rroxscaffold_4G00317700
rosa_rugosa	Rorug01G0108000
rosa_samantha	Rh1BG100400 Rh1DG136400
rosa_wichuraiana	Rw1G010830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	805, 848
Acc65I	GGTACC	1 cut(s)	260
AccB1I	GGYRCC	1 cut(s)	260
AccB7I	CCANNNNNTGG	1 cut(s)	1257
AccBSI	CCGCTC	2 cut(s)	186, 520
AccI	GTMKAC	1 cut(s)	405
AccII	CGCG	3 cut(s)	326, 575, 577
AccIII	TCCGGA	2 cut(s)	188, 1009
AclWI	GGATC	3 cut(s)	626, 660, 1274
AcoI	YGGCCR	2 cut(s)	152, 1185
AcuI	CTGAAG	1 cut(s)	355
AcyI	GRCGYC	5 cut(s)	288, 534, 552, 802, 845
AfaI	GTAC	6 cut(s)	41, 209, 233, 262, 1031, 1193
AfiI	CCNNNNNNNGG	8 cut(s)	161, 432, 694, 738, 829, 1257, 1381, 1382
AgeI	ACCGGT	1 cut(s)	1287
AgsI	TTSAA	2 cut(s)	472, 1019
AjnI	CCWGG	2 cut(s)	823, 1452
AleI	CACNNNNGTG	1 cut(s)	1499
AluBI	AGCT	8 cut(s)	295, 375, 459, 749, 997, 1027, 1106, 1523
AluI	AGCT	8 cut(s)	295, 375, 459, 749, 997, 1027, 1106, 1523
Alw21I	GWGCWC	2 cut(s)	684, 751
Alw26I	GTCTC	1 cut(s)	629
AlwI	GGATC	3 cut(s)	626, 660, 1274
AlwNI	CAGNNNCTG	1 cut(s)	1169
Ama87I	CYCGRG	2 cut(s)	688, 1375
Aor13HI	TCCGGA	2 cut(s)	188, 1009
AoxI	GGCC	6 cut(s)	152, 235, 409, 528, 540, 1185
ApeKI	GCWGC	1 cut(s)	375
ArsI	GACNNNNNNTTYG	2 cut(s)	854, 886
AsiGI	ACCGGT	1 cut(s)	1287
Asp700I	GAANNNNTTC	1 cut(s)	476
Asp718I	GGTACC	1 cut(s)	260
AspLEI	GCGC	5 cut(s)	123, 326, 577, 579, 814
AspS9I	GGNCC	7 cut(s)	140, 236, 410, 423, 442, 562, 1260
AsuC2I	CCSGG	8 cut(s)	138, 147, 413, 1189, 1233, 1324, 1376, 1377
AsuHPI	GGTGA	6 cut(s)	49, 91, 379, 1123, 1339, 1513
AvaI	CYCGRG	2 cut(s)	688, 1375
AvaII	GGWCC	5 cut(s)	140, 423, 442, 562, 1260
BanI	GGYRCC	1 cut(s)	260
BanII	GRGCYC	1 cut(s)	751
Bbv12I	GWGCWC	2 cut(s)	684, 751
BbvI	GCAGC	1 cut(s)	362
BccI	CCATC	2 cut(s)	144, 619
BceAI	ACGGC	7 cut(s)	250, 424, 565, 569, 981, 987, 1333
BcgI	CGANNNNNNTGC	2 cut(s)	1427, 1461
BciT130I	CCWGG	2 cut(s)	825, 1454
BclI	TGATCA	2 cut(s)	273, 1527
BcnI	CCSGG	8 cut(s)	138, 147, 413, 1189, 1233, 1324, 1376, 1377
BcoDI	GTCTC	1 cut(s)	629
BfaI	CTAG	3 cut(s)	1419, 1458, 1536
BfoI	RGCGCY	2 cut(s)	124, 815
BglI	GCCNNNNNGGC	1 cut(s)	151
BisI	GCNGC	4 cut(s)	376, 399, 431, 952
BlpI	GCTNAGC	2 cut(s)	455, 993
BlsI	GCNGC	4 cut(s)	377, 400, 432, 953
Bme18I	GGWCC	5 cut(s)	140, 423, 442, 562, 1260
BmeT110I	CYCGRG	2 cut(s)	688, 1375
BmgT120I	GGNCC	7 cut(s)	140, 236, 410, 423, 442, 562, 1260
BmiI	GGNNCC	3 cut(s)	238, 262, 425
BmsI	GCATC	2 cut(s)	603, 1335
Bpu10I	CCTNAGC	1 cut(s)	281
Bpu1102I	GCTNAGC	2 cut(s)	455, 993
BpuEI	CTTGAG	1 cut(s)	800
BpuMI	CCSGG	8 cut(s)	138, 147, 413, 1189, 1233, 1324, 1376, 1377
BsaAI	YACGTR	1 cut(s)	1127
BsaHI	GRCGYC	5 cut(s)	288, 534, 552, 802, 845
BsaI	GGTCTC	1 cut(s)	629
BsaJI	CCNNGG	8 cut(s)	501, 537, 639, 823, 824, 1323, 1375, 1376
BsaWI	WCCGGW	5 cut(s)	188, 1009, 1287, 1298, 1331
BsaXI	ACNNNNNCTCC	2 cut(s)	1424, 1454
Bsc4I	CCNNNNNNNGG	8 cut(s)	161, 432, 694, 738, 829, 1257, 1381, 1382
Bse118I	RCCGGY	1 cut(s)	1287
Bse1I	ACTGG	2 cut(s)	175, 205
Bse3DI	GCAATG	1 cut(s)	612
BseAI	TCCGGA	2 cut(s)	188, 1009
BseBI	CCWGG	2 cut(s)	825, 1454
BseDI	CCNNGG	8 cut(s)	501, 537, 639, 823, 824, 1323, 1375, 1376
BseGI	GGATG	5 cut(s)	155, 276, 594, 838, 1213
BseLI	CCNNNNNNNGG	8 cut(s)	161, 432, 694, 738, 829, 1257, 1381, 1382
BseMI	GCAATG	1 cut(s)	612
BseMII	CTCAG	4 cut(s)	272, 469, 1007, 1161
BseNI	ACTGG	2 cut(s)	175, 205
BsePI	GCGCGC	1 cut(s)	575
BseRI	GAGGAG	3 cut(s)	10, 647, 1322
BseXI	GCAGC	1 cut(s)	362
BsgI	GTGCAG	1 cut(s)	1448
Bsh1236I	CGCG	3 cut(s)	326, 575, 577
Bsh1285I	CGRYCG	2 cut(s)	1288, 1395
BshFI	GGCC	6 cut(s)	154, 237, 411, 530, 542, 1187
BshNI	GGYRCC	1 cut(s)	260
BshTI	ACCGGT	1 cut(s)	1287
BsiEI	CGRYCG	2 cut(s)	1288, 1395
BsiHKAI	GWGCWC	2 cut(s)	684, 751
BsiHKCI	CYCGRG	2 cut(s)	688, 1375
BslFI	GGGAC	3 cut(s)	409, 770, 903
BslI	CCNNNNNNNGG	8 cut(s)	161, 432, 694, 738, 829, 1257, 1381, 1382
BsmAI	GTCTC	1 cut(s)	629
BsmFI	GGGAC	3 cut(s)	409, 770, 903
BsmI	GAATGC	1 cut(s)	68
BsnI	GGCC	6 cut(s)	154, 237, 411, 530, 542, 1187
Bso31I	GGTCTC	1 cut(s)	629
BsoBI	CYCGRG	2 cut(s)	688, 1375
Bsp1286I	GDGCHC	2 cut(s)	684, 751
Bsp13I	TCCGGA	2 cut(s)	188, 1009
Bsp1407I	TGTACA	1 cut(s)	1029
Bsp143I	GATC	8 cut(s)	217, 273, 312, 618, 652, 874, 1279, 1527
Bsp1720I	GCTNAGC	2 cut(s)	455, 993
Bsp19I	CCATGG	1 cut(s)	501
BspANI	GGCC	6 cut(s)	154, 237, 411, 530, 542, 1187
BspCNI	CTCAG	4 cut(s)	273, 468, 1006, 1162
BspEI	TCCGGA	2 cut(s)	188, 1009
BspFNI	CGCG	3 cut(s)	326, 575, 577
BspLI	GGNNCC	3 cut(s)	238, 262, 425
BspPI	GGATC	3 cut(s)	626, 660, 1274
BspT107I	GGYRCC	1 cut(s)	260
BspTNI	GGTCTC	1 cut(s)	629
BsrBI	CCGCTC	2 cut(s)	186, 520
BsrDI	GCAATG	1 cut(s)	612
BsrFI	RCCGGY	1 cut(s)	1287
BsrGI	TGTACA	1 cut(s)	1029
BsrI	ACTGG	2 cut(s)	175, 205
BssAI	RCCGGY	1 cut(s)	1287
BssECI	CCNNGG	8 cut(s)	501, 537, 639, 823, 824, 1323, 1375, 1376
BssHII	GCGCGC	1 cut(s)	575
BssMI	GATC	8 cut(s)	217, 273, 312, 618, 652, 874, 1279, 1527
BssNI	GRCGYC	5 cut(s)	288, 534, 552, 802, 845
BssT1I	CCWWGG	2 cut(s)	501, 537
Bst2UI	CCWGG	2 cut(s)	825, 1454
Bst4CI	ACNGT	5 cut(s)	391, 640, 670, 1396, 1498
Bst6I	CTCTTC	2 cut(s)	176, 737
BstACI	GRCGYC	5 cut(s)	288, 534, 552, 802, 845
BstAUI	TGTACA	1 cut(s)	1029
BstBAI	YACGTR	1 cut(s)	1127
BstC8I	GCNNGC	4 cut(s)	184, 577, 1104, 1416
BstDEI	CTNAG	5 cut(s)	281, 455, 993, 1170, 1517
BstDSI	CCRYGG	2 cut(s)	501, 639
BstEII	GGTNACC	1 cut(s)	1327
BstF5I	GGATG	5 cut(s)	155, 276, 594, 838, 1213
BstFNI	CGCG	3 cut(s)	326, 575, 577
BstH2I	RGCGCY	2 cut(s)	124, 815
BstHHI	GCGC	5 cut(s)	123, 326, 577, 579, 814
BstKTI	GATC	8 cut(s)	220, 276, 315, 621, 655, 877, 1282, 1530
BstMAI	GTCTC	1 cut(s)	629
BstMBI	GATC	8 cut(s)	217, 273, 312, 618, 652, 874, 1279, 1527
BstMCI	CGRYCG	2 cut(s)	1288, 1395
BstMWI	GCNNNNNNNGC	7 cut(s)	151, 332, 427, 465, 581, 1099, 1454
BstNI	CCWGG	2 cut(s)	825, 1454
BstNSI	RCATGY	2 cut(s)	454, 1403
BstPI	GGTNACC	1 cut(s)	1327
BstSNI	TACGTA	1 cut(s)	1127
BstUI	CGCG	3 cut(s)	326, 575, 577
BstV1I	GCAGC	1 cut(s)	362
BsuRI	GGCC	6 cut(s)	154, 237, 411, 530, 542, 1187
BtgI	CCRYGG	2 cut(s)	501, 639
BtsCI	GGATG	5 cut(s)	155, 276, 594, 838, 1213
BtsIMutI	CAGTG	2 cut(s)	954, 1494
Cac8I	GCNNGC	4 cut(s)	184, 577, 1104, 1416
CaiI	CAGNNNCTG	1 cut(s)	1169
CfoI	GCGC	5 cut(s)	123, 326, 577, 579, 814
Cfr10I	RCCGGY	1 cut(s)	1287
Cfr13I	GGNCC	7 cut(s)	140, 236, 410, 423, 442, 562, 1260
Cfr9I	CCCGGG	1 cut(s)	1375
CpoI	CGGWCCG	1 cut(s)	140
CseI	GACGC	3 cut(s)	296, 541, 542
Csp6I	GTAC	6 cut(s)	40, 208, 232, 261, 1030, 1192
CspAI	ACCGGT	1 cut(s)	1287
CspI	CGGWCCG	1 cut(s)	140
CviAII	CATG	8 cut(s)	271, 439, 451, 502, 911, 1160, 1387, 1400
CviQI	GTAC	6 cut(s)	40, 208, 232, 261, 1030, 1192
DdeI	CTNAG	5 cut(s)	281, 455, 993, 1170, 1517
DpnI	GATC	8 cut(s)	219, 275, 314, 620, 654, 876, 1281, 1529
DpnII	GATC	8 cut(s)	217, 273, 312, 618, 652, 874, 1279, 1527
EaeI	YGGCCR	2 cut(s)	152, 1185
Eam1104I	CTCTTC	2 cut(s)	176, 737
EarI	CTCTTC	2 cut(s)	176, 737
EciI	GGCGGA	3 cut(s)	132, 448, 1328
Ecl136II	GAGCTC	1 cut(s)	749
Eco105I	TACGTA	1 cut(s)	1127
Eco130I	CCWWGG	2 cut(s)	501, 537
Eco24I	GRGCYC	1 cut(s)	751
Eco31I	GGTCTC	1 cut(s)	629
Eco47I	GGWCC	5 cut(s)	140, 423, 442, 562, 1260
Eco53kI	GAGCTC	1 cut(s)	749
Eco57I	CTGAAG	1 cut(s)	355
Eco88I	CYCGRG	2 cut(s)	688, 1375
Eco91I	GGTNACC	1 cut(s)	1327
EcoICRI	GAGCTC	1 cut(s)	749
EcoO65I	GGTNACC	1 cut(s)	1327
EcoRII	CCWGG	2 cut(s)	823, 1452
EcoT14I	CCWWGG	2 cut(s)	501, 537
EcoT38I	GRGCYC	1 cut(s)	751
ErhI	CCWWGG	2 cut(s)	501, 537
FaeI	CATG	8 cut(s)	274, 442, 454, 505, 914, 1163, 1390, 1403
FaqI	GGGAC	3 cut(s)	409, 770, 903
FatI	CATG	8 cut(s)	270, 438, 450, 501, 910, 1159, 1386, 1399
FauI	CCCGC	1 cut(s)	191
FbaI	TGATCA	2 cut(s)	273, 1527
FblI	GTMKAC	1 cut(s)	405
Fnu4HI	GCNGC	4 cut(s)	376, 399, 431, 952
FokI	GGATG	5 cut(s)	162, 263, 581, 845, 1220
FriOI	GRGCYC	1 cut(s)	751
Fsp4HI	GCNGC	4 cut(s)	376, 399, 431, 952
FspBI	CTAG	3 cut(s)	1419, 1458, 1536
GlaI	GCGC	5 cut(s)	122, 325, 576, 578, 813
GluI	GCNGC	4 cut(s)	376, 399, 431, 952
HaeII	RGCGCY	2 cut(s)	124, 815
HaeIII	GGCC	6 cut(s)	154, 237, 411, 530, 542, 1187
HgaI	GACGC	3 cut(s)	296, 541, 542
HhaI	GCGC	5 cut(s)	123, 326, 577, 579, 814
Hin1I	GRCGYC	5 cut(s)	288, 534, 552, 802, 845
Hin1II	CATG	8 cut(s)	274, 442, 454, 505, 914, 1163, 1390, 1403
Hin6I	GCGC	5 cut(s)	121, 324, 575, 577, 812
HinP1I	GCGC	5 cut(s)	121, 324, 575, 577, 812
HincII	GTYRAC	2 cut(s)	394, 1069
HindII	GTYRAC	2 cut(s)	394, 1069
HinfI	GANTC	3 cut(s)	772, 1247, 1539
HphI	GGTGA	6 cut(s)	49, 91, 379, 1123, 1339, 1513
Hpy166II	GTNNAC	7 cut(s)	301, 387, 394, 406, 1069, 1130, 1494
Hpy188I	TCNGA	8 cut(s)	482, 491, 657, 832, 937, 945, 1243, 1544
Hpy8I	GTNNAC	7 cut(s)	301, 387, 394, 406, 1069, 1130, 1494
Hpy99I	CGWCG	9 cut(s)	290, 536, 557, 804, 807, 847, 967, 973, 1207
HpyAV	CCTTC	5 cut(s)	25, 313, 466, 915, 1108
HpyCH4III	ACNGT	5 cut(s)	391, 640, 670, 1396, 1498
HpyCH4IV	ACGT	3 cut(s)	802, 845, 1126
HpyCH4V	TGCA	7 cut(s)	68, 378, 594, 1102, 1163, 1399, 1429
HpyF10VI	GCNNNNNNNGC	7 cut(s)	151, 332, 427, 465, 581, 1099, 1454
HpyF3I	CTNAG	5 cut(s)	281, 455, 993, 1170, 1517
HpySE526I	ACGT	3 cut(s)	802, 845, 1126
Hsp92I	GRCGYC	5 cut(s)	288, 534, 552, 802, 845
Hsp92II	CATG	8 cut(s)	274, 442, 454, 505, 914, 1163, 1390, 1403
HspAI	GCGC	5 cut(s)	121, 324, 575, 577, 812
Kpn2I	TCCGGA	2 cut(s)	188, 1009
KpnI	GGTACC	1 cut(s)	264
Ksp22I	TGATCA	2 cut(s)	273, 1527
Kzo9I	GATC	8 cut(s)	217, 273, 312, 618, 652, 874, 1279, 1527
LmnI	GCTCC	2 cut(s)	191, 464
Lsp1109I	GCAGC	1 cut(s)	362
LweI	GCATC	2 cut(s)	603, 1335
MaeI	CTAG	3 cut(s)	1419, 1458, 1536
MaeII	ACGT	3 cut(s)	802, 845, 1126
MaeIII	GTNAC	3 cut(s)	922, 1165, 1327
MalI	GATC	8 cut(s)	219, 275, 314, 620, 654, 876, 1281, 1529
MbiI	CCGCTC	2 cut(s)	186, 520
MboI	GATC	8 cut(s)	217, 273, 312, 618, 652, 874, 1279, 1527
MboII	GAAGA	8 cut(s)	82, 163, 193, 361, 754, 891, 1477, 1519
MhlI	GDGCHC	2 cut(s)	684, 751
MluCI	AATT	6 cut(s)	917, 987, 1038, 1253, 1335, 1484
MlyI	GAGTC	1 cut(s)	1241
MmeI	TCCRAC	2 cut(s)	810, 1287
MroI	TCCGGA	2 cut(s)	188, 1009
MroXI	GAANNNNTTC	1 cut(s)	476
MseI	TTAA	6 cut(s)	86, 525, 674, 716, 1041, 1338
MslI	CAYNNNNRTG	1 cut(s)	1499
MspA1I	CMGCKG	2 cut(s)	375, 401
Mva1269I	GAATGC	1 cut(s)	68
MvaI	CCWGG	2 cut(s)	825, 1454
MvnI	CGCG	3 cut(s)	326, 575, 577
MwoI	GCNNNNNNNGC	7 cut(s)	151, 332, 427, 465, 581, 1099, 1454
NciI	CCSGG	8 cut(s)	138, 147, 413, 1189, 1233, 1324, 1376, 1377
NcoI	CCATGG	1 cut(s)	501
NdeII	GATC	8 cut(s)	217, 273, 312, 618, 652, 874, 1279, 1527
NlaIII	CATG	8 cut(s)	274, 442, 454, 505, 914, 1163, 1390, 1403
NlaIV	GGNNCC	3 cut(s)	238, 262, 425
NmuCI	GTSAC	2 cut(s)	922, 1327
NspI	RCATGY	2 cut(s)	454, 1403
OliI	CACNNNNGTG	1 cut(s)	1499
PasI	CCCWGGG	1 cut(s)	824
PauI	GCGCGC	1 cut(s)	575
PcsI	WCGNNNNNNNCGW	3 cut(s)	294, 627, 636
PctI	GAATGC	1 cut(s)	68
PdmI	GAANNNNTTC	1 cut(s)	476
PfeI	GAWTC	2 cut(s)	772, 1539
PflFI	GACNNNGTC	1 cut(s)	551
PflMI	CCANNNNNTGG	1 cut(s)	1257
PinAI	ACCGGT	1 cut(s)	1287
PkrI	GCNGC	4 cut(s)	377, 400, 432, 953
PleI	GAGTC	1 cut(s)	1241
PpsI	GAGTC	1 cut(s)	1241
Ppu21I	YACGTR	1 cut(s)	1127
Psp124BI	GAGCTC	1 cut(s)	751
Psp6I	CCWGG	2 cut(s)	823, 1452
PspEI	GGTNACC	1 cut(s)	1327
PspGI	CCWGG	2 cut(s)	823, 1452
PspN4I	GGNNCC	3 cut(s)	238, 262, 425
PspPI	GGNCC	7 cut(s)	140, 236, 410, 423, 442, 562, 1260
PstNI	CAGNNNCTG	1 cut(s)	1169
PsyI	GACNNNGTC	1 cut(s)	551
PteI	GCGCGC	1 cut(s)	575
PvuII	CAGCTG	1 cut(s)	375
RsaI	GTAC	6 cut(s)	41, 209, 233, 262, 1031, 1193
RsaNI	GTAC	6 cut(s)	40, 208, 232, 261, 1030, 1192
RseI	CAYNNNNRTG	1 cut(s)	1499
Rsr2I	CGGWCCG	1 cut(s)	140
RsrII	CGGWCCG	1 cut(s)	140
SacI	GAGCTC	1 cut(s)	751
SaqAI	TTAA	6 cut(s)	86, 525, 674, 716, 1041, 1338
SatI	GCNGC	4 cut(s)	376, 399, 431, 952
Sau3AI	GATC	8 cut(s)	217, 273, 312, 618, 652, 874, 1279, 1527
Sau96I	GGNCC	7 cut(s)	140, 236, 410, 423, 442, 562, 1260
SchI	GAGTC	1 cut(s)	1241
SduI	GDGCHC	2 cut(s)	684, 751
SfaNI	GCATC	2 cut(s)	603, 1335
SinI	GGWCC	5 cut(s)	140, 423, 442, 562, 1260
SmaI	CCCGGG	1 cut(s)	1377
SmiMI	CAYNNNNRTG	1 cut(s)	1499
SmlI	CTYRAG	1 cut(s)	815
SmoI	CTYRAG	1 cut(s)	815
SnaBI	TACGTA	1 cut(s)	1127
Sse9I	AATT	6 cut(s)	917, 987, 1038, 1253, 1335, 1484
SspMI	CTAG	3 cut(s)	1419, 1458, 1536
SstI	GAGCTC	1 cut(s)	751
StyI	CCWWGG	2 cut(s)	501, 537
TaaI	ACNGT	5 cut(s)	391, 640, 670, 1396, 1498
TaiI	ACGT	3 cut(s)	805, 848, 1129
TaqII	GACCGA	1 cut(s)	550
TasI	AATT	6 cut(s)	917, 987, 1038, 1253, 1335, 1484
TatI	WGTACW	1 cut(s)	1029
TauI	GCSGC	3 cut(s)	401, 433, 954
TfiI	GAWTC	2 cut(s)	772, 1539
Tru1I	TTAA	6 cut(s)	86, 525, 674, 716, 1041, 1338
Tru9I	TTAA	6 cut(s)	86, 525, 674, 716, 1041, 1338
TscAI	CASTG	2 cut(s)	961, 1501
TseFI	GTSAC	2 cut(s)	922, 1327
TseI	GCWGC	1 cut(s)	375
Tsp45I	GTSAC	2 cut(s)	922, 1327
TspDTI	ATGAA	5 cut(s)	677, 769, 781, 1394, 1520
TspGWI	ACGGA	1 cut(s)	1220
TspMI	CCCGGG	1 cut(s)	1375
TspRI	CASTG	2 cut(s)	961, 1501
Tth111I	GACNNNGTC	1 cut(s)	551
Van91I	CCANNNNNTGG	1 cut(s)	1257
VpaK11BI	GGWCC	5 cut(s)	140, 423, 442, 562, 1260
XceI	RCATGY	2 cut(s)	454, 1403
XmaI	CCCGGG	1 cut(s)	1375
XmiI	GTMKAC	1 cut(s)	405
XmnI	GAANNNNTTC	1 cut(s)	476
XspI	CTAG	3 cut(s)	1419, 1458, 1536
ZraI	GACGTC	2 cut(s)	803, 846

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.