Rorug01G0108000

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0015427
Physical Location & Seq
Reverse (-)
19364718 .. 19366542
1825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0108000.1

Sequence Viewer

Length: 657 bp
ATGTCTGCAGATCCGACACCGGGTTCAGGTTTGAAAATGGGTTCGGTTTGCCGGAATCGAGGGAGATCCAGGCAGGTCGGTTGCTCGGTCTGGTTTCGTACGGCCACATCGACGACGACGGTGGAGGAGATCCAGGCAGGTCAATCGGTTGCTCGGTCTGGCTCCGTACGGCCACATCGACGACATCCGTATGGCCACATCGACGACGACGACGGTGGAAGAGATCCAGGCAGAAACTCACGAGTCTGGGAGGGGAAGAGTGCTTTGGCTTGTGATGTTATGCTTAGAGCTGCTGCTCGTCTGCAAGCGTTTCGAGCTCACAATACCAAGTCTGCTTTTGCTAGTGTTCGAAGAAGAAGAGTGCAAAAATGGGTGGCTCCTTCAGCAGGGTTGTTAAAAATCAATGTTGATGGCTCCTTTCATCATGAAACTAGGAAAAGCGGTTTTGGTTTTGTGATAAGAGATTCAAGTGGTGCAATGTTAGCTGATGGTGCAGGTCCCTTGCGCGGACTGATATCATCAGAACATGCAGAAGTTTTGGCGTGTAGGAATGCAATGAACTTTGCAATTGATCATGGCTTCGTTCCAGCTTTGTTGGAGATTGATGCACAAGAGGTGCAACGTCAACTCGCTTCCCAGGCTAGCCTCAATACTTAA

Protein Analysis

218

Amino Acids

23.58

Weight (kDa)

10.31

Isoelectric Point (pI)

52.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 135 - 214 2.3e-15 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10570 AT5G04660
fragaria_vesca FvH4_7g05960
malus_domestica MD02G1247800.v1.1 MD07G1068700.v1.1
prunus_persica Prupe.2G086300_v2.0.a1
pyrus_communis pycom02g21180 pycom07g05280
rosa_chinensis RchiOBHm_Chr1g0333181
rosa_laevigata RLG00000029620
rosa_multiflora Rmu_sc0001214.1_g000041
rosa_roxburghii Rroxscaffold_4G00317700
rosa_rugosa Rorug01G0108000
rosa_samantha Rh1BG100400 Rh1DG136400
rosa_wichuraiana Rw1G010830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 64, 128, 485
AccII CGCG 1 cut(s) 507
AciI CCGC 2 cut(s) 441, 507
AclWI GGATC 4 cut(s) 5, 60, 124, 218
AcoI YGGCCR 3 cut(s) 102, 170, 193
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 2 cut(s) 100, 168
AfiI CCNNNNNNNGG 4 cut(s) 20, 26, 386, 506
AgsI TTSAA 2 cut(s) 34, 468
AjnI CCWGG 4 cut(s) 68, 132, 226, 636
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AluBI AGCT 4 cut(s) 290, 317, 485, 590
AluI AGCT 4 cut(s) 290, 317, 485, 590
Alw21I GWGCWC 1 cut(s) 319
AlwI GGATC 4 cut(s) 5, 60, 124, 218
AoxI GGCC 3 cut(s) 102, 170, 193
ApeKI GCWGC 2 cut(s) 290, 293
AspLEI GCGC 1 cut(s) 507
AspS9I GGNCC 1 cut(s) 497
AsuC2I CCSGG 1 cut(s) 21
AsuII TTCGAA 1 cut(s) 349
AsuNHI GCTAGC 1 cut(s) 641
AvaII GGWCC 1 cut(s) 497
BalI TGGCCA 1 cut(s) 195
BanII GRGCYC 1 cut(s) 319
BauI CACGAG 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 2 cut(s) 277, 280
BccI CCATC 2 cut(s) 404, 482
BceAI ACGGC 2 cut(s) 117, 185
BcgI CGANNNNNNTGC 4 cut(s) 126, 160, 293, 327
BciT130I CCWGG 4 cut(s) 70, 134, 228, 638
BclI TGATCA 1 cut(s) 571
BcnI CCSGG 1 cut(s) 21
BfaI CTAG 3 cut(s) 342, 432, 642
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 3 cut(s) 64, 128, 485
BisI GCNGC 2 cut(s) 291, 294
BlsI GCNGC 2 cut(s) 292, 295
Bme1390I CCNGG 5 cut(s) 21, 70, 134, 228, 638
Bme18I GGWCC 1 cut(s) 497
BmgT120I GGNCC 1 cut(s) 497
BmiI GGNNCC 4 cut(s) 163, 378, 415, 499
BmrFI CCNGG 5 cut(s) 21, 70, 134, 228, 638
BmsI GCATC 1 cut(s) 595
BmtI GCTAGC 1 cut(s) 645
Bpu14I TTCGAA 1 cut(s) 349
BpuMI CCSGG 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 636
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 26, 386, 506
Bse3DI GCAATG 2 cut(s) 483, 561
BseBI CCWGG 4 cut(s) 70, 134, 228, 638
BseDI CCNNGG 1 cut(s) 636
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 4 cut(s) 20, 26, 386, 506
BseMI GCAATG 2 cut(s) 483, 561
BseRI GAGGAG 1 cut(s) 140
BseXI GCAGC 2 cut(s) 277, 280
BsgI GTGCAG 1 cut(s) 513
Bsh1236I CGCG 1 cut(s) 507
BshFI GGCC 3 cut(s) 104, 172, 195
BsiHKAI GWGCWC 1 cut(s) 319
BsiSI CCGG 2 cut(s) 20, 52
BsiWI CGTACG 2 cut(s) 98, 166
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 4 cut(s) 20, 26, 386, 506
BsmFI GGGAC 1 cut(s) 483
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 3 cut(s) 104, 172, 195
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 5 cut(s) 10, 65, 129, 223, 571
BspACI CCGC 2 cut(s) 441, 507
BspANI GGCC 3 cut(s) 104, 172, 195
BspFNI CGCG 1 cut(s) 507
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 4 cut(s) 163, 378, 415, 499
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 3 cut(s) 64, 128, 485
BspOI GCTAGC 1 cut(s) 645
BspPI GGATC 4 cut(s) 5, 60, 124, 218
BspT104I TTCGAA 1 cut(s) 349
BsrDI GCAATG 2 cut(s) 483, 561
BssECI CCNNGG 1 cut(s) 636
BssMI GATC 5 cut(s) 10, 65, 129, 223, 571
BssSI CACGAG 1 cut(s) 240
Bst2BI CACGAG 1 cut(s) 240
Bst2UI CCWGG 4 cut(s) 70, 134, 228, 638
Bst4CI ACNGT 2 cut(s) 121, 215
Bst6I CTCTTC 3 cut(s) 214, 251, 352
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 306, 643
BstDEI CTNAG 1 cut(s) 284
BstENI CCTNNNNNAGG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 184
BstFNI CGCG 1 cut(s) 507
BstHHI GCGC 1 cut(s) 507
BstKTI GATC 5 cut(s) 13, 68, 132, 226, 574
BstMBI GATC 5 cut(s) 10, 65, 129, 223, 571
BstMWI GCNNNNNNNGC 5 cut(s) 314, 383, 482, 491, 638
BstNI CCWGG 4 cut(s) 70, 134, 228, 638
BstNSI RCATGY 1 cut(s) 530
BstSCI CCNGG 5 cut(s) 19, 68, 132, 226, 636
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 2 cut(s) 277, 280
BstX2I RGATCY 4 cut(s) 10, 65, 129, 223
BstYI RGATCY 4 cut(s) 10, 65, 129, 223
BsuRI GGCC 3 cut(s) 104, 172, 195
BtsCI GGATG 1 cut(s) 184
BveI ACCTGC 3 cut(s) 64, 128, 485
Cac8I GCNNGC 2 cut(s) 306, 643
CciI TCATGA 1 cut(s) 424
CfoI GCGC 1 cut(s) 507
Cfr13I GGNCC 1 cut(s) 497
Csp6I GTAC 2 cut(s) 99, 167
CviAII CATG 3 cut(s) 425, 527, 575
CviQI GTAC 2 cut(s) 99, 167
DdeI CTNAG 1 cut(s) 284
DpnI GATC 5 cut(s) 12, 67, 131, 225, 573
DpnII GATC 5 cut(s) 10, 65, 129, 223, 571
EaeI YGGCCR 3 cut(s) 102, 170, 193
Eam1104I CTCTTC 3 cut(s) 214, 251, 352
EarI CTCTTC 3 cut(s) 214, 251, 352
Ecl136II GAGCTC 1 cut(s) 317
Eco24I GRGCYC 1 cut(s) 319
Eco32I GATATC 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 497
Eco53kI GAGCTC 1 cut(s) 317
Eco57I CTGAAG 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 317
EcoNI CCTNNNNNAGG 1 cut(s) 384
EcoO109I RGGNCCY 1 cut(s) 497
EcoRII CCWGG 4 cut(s) 68, 132, 226, 636
EcoRV GATATC 1 cut(s) 516
EcoT38I GRGCYC 1 cut(s) 319
FaeI CATG 3 cut(s) 428, 530, 578
FaiI YATR 5 cut(s) 192, 281, 426, 528, 576
FaqI GGGAC 1 cut(s) 483
FatI CATG 3 cut(s) 424, 526, 574
FbaI TGATCA 1 cut(s) 571
Fnu4HI GCNGC 2 cut(s) 291, 294
FokI GGATG 1 cut(s) 171
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 2 cut(s) 291, 294
FspBI CTAG 3 cut(s) 342, 432, 642
GlaI GCGC 1 cut(s) 506
GluI GCNGC 2 cut(s) 291, 294
HaeIII GGCC 3 cut(s) 104, 172, 195
HapII CCGG 2 cut(s) 20, 52
HhaI GCGC 1 cut(s) 507
Hin1II CATG 3 cut(s) 428, 530, 578
Hin6I GCGC 1 cut(s) 505
HinP1I GCGC 1 cut(s) 505
HincII GTYRAC 1 cut(s) 626
HindII GTYRAC 1 cut(s) 626
HinfI GANTC 3 cut(s) 55, 243, 464
HpaII CCGG 2 cut(s) 20, 52
Hpy166II GTNNAC 1 cut(s) 626
Hpy188I TCNGA 2 cut(s) 15, 523
Hpy188III TCNNGA 2 cut(s) 240, 425
Hpy8I GTNNAC 1 cut(s) 626
Hpy99I CGWCG 8 cut(s) 115, 118, 121, 183, 206, 209, 212, 215
HpyAV CCTTC 1 cut(s) 390
HpyCH4III ACNGT 2 cut(s) 121, 215
HpyCH4IV ACGT 1 cut(s) 622
HpyF10VI GCNNNNNNNGC 5 cut(s) 314, 383, 482, 491, 638
HpyF3I CTNAG 1 cut(s) 284
HpySE526I ACGT 1 cut(s) 622
Hsp92II CATG 3 cut(s) 428, 530, 578
HspAI GCGC 1 cut(s) 505
Ksp22I TGATCA 1 cut(s) 571
Kzo9I GATC 5 cut(s) 10, 65, 129, 223, 571
LmnI GCTCC 3 cut(s) 167, 382, 419
Lsp1109I GCAGC 2 cut(s) 277, 280
LweI GCATC 1 cut(s) 595
MaeI CTAG 3 cut(s) 342, 432, 642
MaeII ACGT 1 cut(s) 622
MalI GATC 5 cut(s) 12, 67, 131, 225, 573
MboI GATC 5 cut(s) 10, 65, 129, 223, 571
MboII GAAGA 5 cut(s) 231, 268, 363, 366, 369
MfeI CAATTG 1 cut(s) 567
MflI RGATCY 4 cut(s) 10, 65, 129, 223
MhlI GDGCHC 1 cut(s) 319
MlsI TGGCCA 1 cut(s) 195
MluCI AATT 1 cut(s) 567
MluNI TGGCCA 1 cut(s) 195
MlyI GAGTC 1 cut(s) 252
MmeI TCCRAC 2 cut(s) 38, 576
MnlI CCTC 5 cut(s) 53, 118, 244, 607, 656
Mox20I TGGCCA 1 cut(s) 195
MscI TGGCCA 1 cut(s) 195
MseI TTAA 2 cut(s) 395, 655
MslI CAYNNNNRTG 1 cut(s) 189
Msp20I TGGCCA 1 cut(s) 195
MspI CCGG 2 cut(s) 20, 52
MspR9I CCNGG 5 cut(s) 21, 70, 134, 228, 638
MunI CAATTG 1 cut(s) 567
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 4 cut(s) 70, 134, 228, 638
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 5 cut(s) 314, 383, 482, 491, 638
NciI CCSGG 1 cut(s) 21
NdeII GATC 5 cut(s) 10, 65, 129, 223, 571
NheI GCTAGC 1 cut(s) 641
NlaIII CATG 3 cut(s) 428, 530, 578
NlaIV GGNNCC 4 cut(s) 163, 378, 415, 499
NspI RCATGY 1 cut(s) 530
NspV TTCGAA 1 cut(s) 349
PagI TCATGA 1 cut(s) 424
PcsI WCGNNNNNNNCGW 3 cut(s) 107, 175, 207
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 2 cut(s) 55, 464
Pfl23II CGTACG 2 cut(s) 98, 166
PkrI GCNGC 2 cut(s) 292, 295
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
PpuMI RGGWCCY 1 cut(s) 497
Psp124BI GAGCTC 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 497
Psp6I CCWGG 4 cut(s) 68, 132, 226, 636
PspGI CCWGG 4 cut(s) 68, 132, 226, 636
PspLI CGTACG 2 cut(s) 98, 166
PspN4I GGNNCC 4 cut(s) 163, 378, 415, 499
PspPI GGNCC 1 cut(s) 497
PspPPI RGGWCCY 1 cut(s) 497
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 4 cut(s) 10, 65, 129, 223
RsaI GTAC 2 cut(s) 100, 168
RsaNI GTAC 2 cut(s) 99, 167
RseI CAYNNNNRTG 1 cut(s) 189
SacI GAGCTC 1 cut(s) 319
SaqAI TTAA 2 cut(s) 395, 655
SatI GCNGC 2 cut(s) 291, 294
Sau3AI GATC 5 cut(s) 10, 65, 129, 223, 571
Sau96I GGNCC 1 cut(s) 497
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 5 cut(s) 21, 70, 134, 228, 638
SduI GDGCHC 1 cut(s) 319
SfaNI GCATC 1 cut(s) 595
SfcI CTRYAG 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 349
SinI GGWCC 1 cut(s) 497
SmiMI CAYNNNNRTG 1 cut(s) 189
Sse9I AATT 1 cut(s) 567
SsiI CCGC 2 cut(s) 441, 507
SspMI CTAG 3 cut(s) 342, 432, 642
SstI GAGCTC 1 cut(s) 319
StyD4I CCNGG 5 cut(s) 19, 68, 132, 226, 636
TaaI ACNGT 2 cut(s) 121, 215
TaiI ACGT 1 cut(s) 625
TaqI TCGA 6 cut(s) 58, 110, 178, 201, 313, 349
TaqII GACCGA 2 cut(s) 76, 144
TasI AATT 1 cut(s) 567
TfiI GAWTC 2 cut(s) 55, 464
Tru1I TTAA 2 cut(s) 395, 655
Tru9I TTAA 2 cut(s) 395, 655
TseI GCWGC 2 cut(s) 290, 293
TspDTI ATGAA 3 cut(s) 410, 441, 572
TspGWI ACGGA 2 cut(s) 154, 177
VpaK11BI GGWCC 1 cut(s) 497
XagI CCTNNNNNAGG 1 cut(s) 384
XceI RCATGY 1 cut(s) 530
XspI CTAG 3 cut(s) 342, 432, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.