Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0012973

Physical Location & Seq

Genomic Coordinates Forward (+)

19476634 .. 19484978

Gene Structure 8345 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1161400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2025 bp

ATGTCGGATGACATGCTCATCCATTTCTCCTCCAACTCCTCCAACCAGTCGGACCACTCTCTGCCCACCAAGATTGCCAAGCTCGAAGCCCGAATGGTCGGCAAGGCCTCCTCCGCCCTTCCTGCTCTCCAGCAGCAACAGCAGCAGCAGCCGAGATTCACCACGGCTGACTTGGCCGAGCCTTCAACTTCGAGTGATTCCGACGATGATAATGGAGGGGAGTTTCTGATACAAGCCAACACTCAGAAGCGCCAGAAACTTCAAGAAGATGGTAATTCAACTGTTCTTGAACATGTTGAGGCAGTGGTTGATGGGAGGCAGATGGTTGTAGAAACTGTTGATACCAAAGCAACTTCTGATGTAAATCAAAAAAAGCATGGTCGTGGTAGGGGGAATTCAATTTCAGGTAGGGTTCGTGGTTCAAGAGTCAGTGATCAGACAAGATTATTAGTAACTCCTTCGACAGTTTCACCTCTCAATGGTCAGTCTGAGAACCCATACAATAAGGATAGGGGTAGCAGGCCTAAAGAGCAGTTTCGGAATGACAACCGCTCTTCATTAGATGAGATTGCATCTTTACGTGCAACAGTTGCTGCTCTAGAGGAGGACTTGCGAAAATCACGTCAAGAGGCCTCTGATTATCAGAATCTTTGCCGTCAATCAGAGAAGGAATTGAATGATGTAAAAGAGCTTGAACAGAAAATGAAGCCAAAGAGAACAAAAGTGATCTCTGATCTGCTGATATCTGTTTCGAAAGCAGAAAGGCAGGAAGCAAGGTTAAAAGTACGACAGGACTCTTTGAGGCTTGGCAATGTGGGTGTCATCAGAGCTGGAACTGTCATATCTGAGACATGGGAAGATGGGCAAGCTTTGAAAGATCTCAATGCTCATCTTAGACAATTATTAGAAACTAAGGAGGCTGTTGAGCGGCAGCGAAAGTCATTGAAGAAAAAGCAAAGTGATAAGGGTGATGGAGTTGATGCTGAAACTGGAATGCAAGAAGAACTTCTAGTCCAGGATGAGATCTATAAATCCCGTCTAGCCAGCATCAAACGAGAGGAGGAAGTTATCCTGCGTGAAAGAGACCGATATGAACTGGAGAAGGGAAGGCTTATACGTGAAATGAAACGCATACGAGATGAGGATGGTTCCCGTTTTAACAATTTTCAGATTCTCAACCACAGATATGCCCTCTTAAATCTCCTTGGCAAGGGGGGATTCAGTGAAGTTTACAAGGCATTTGACTTGGTAGGGCATAGATATGTTGCATGTAAACTTCATGGTTTAAATGCTCAGTGGAGTGAAGAGAAGAAGCAAAGTTACATACGACATGCAATCCGGGAGTACAATATACACAAGACTTTGGTGCATCATCACATTGTTCGACTTTGGGACATTTTTGAGATTGATCAAAATACATTCTGCACTGTCTTAGAGTATTGCAGTGGGAAAGATCTTGATGCTGTCCTTAAAGCAACACCTGTGTTACCCGAGAGGGAAGCTAGGATCATTATCTTTCAGATATTTCAAGGTCTTATATACTTGAACAAAAGAACACAGAAGATTATACATTATGATTTGAAGCCTGGAAATGTCCTATTTGACAAACTCGGGGTTGCAAAAGTGACTGACTTTGGTCTTAGCAAGATAGTGGAGGATGATGTCGGATCCCAGGGTATGGAACTAACATCCCAGGGTGCTGGGACTTATTGGTATTTACCTCCTGAATGCTTCGAGCTCAGCAAGACACCTCTTATATCATCGAAGGTTGACGTCTGGTCAGCAGGTATTCTGTTATACCAAATGCTATTTGGAAGACGCCCTTTTGGGCATGATCAGACCCAGGAAAGAATACTACGGGAGGACACAATTATTAAAGCTCGAAAAGTTGAATTTCCATCCAGACCTGCCATCTCAAATGAAGCAAAGGAATTTATTCGCCGTTGTCTAACATATAATCAAGCAGAGAGGCCAGATGTTATAGGGATTGCTCAAGATCCATTTCTCACATACTCAAAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0060147 GO:0060255 GO:0060966 GO:0060968 GO:0065007 GO:0071704 GO:0140096 GO:1900368 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

675

Amino Acids

76.86

Weight (kDa)

8.68

Isoelectric Point (pI)

44.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	396 - 669	7.7e-58	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	399 - 663	3.2e-31	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20930
fragaria_vesca	FvH4_3g26940
malus_domestica	MD03G1161400.v1.1 MD11G1177500.v1.1
prunus_persica	Prupe.6G149600_v2.0.a1 Prupe.6G149600_v2.0.a1 Prupe.6G149600_v2.0.a1
pyrus_communis	pycom11g15140
rosa_chinensis	RchiOBHm_Chr5g0049381
rosa_laevigata	RLG00000034615
rosa_multiflora	Rmu_sc0014052.1_g000012
rosa_roxburghii	Rroxscaffold_1G00032310
rosa_rugosa	Rorug05G0248100
rosa_samantha	Rh5AG324500 Rh5BG335600 Rh5CG360300 Rh5DG347900
rosa_wichuraiana	Rw5G030660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1776
Acc36I	ACCTGC	2 cut(s)	1775, 1915
AccB7I	CCANNNNNTGG	1 cut(s)	1678
AccBSI	CCGCTC	2 cut(s)	552, 928
AciI	CCGC	3 cut(s)	114, 550, 928
AclWI	GGATC	4 cut(s)	1514, 1662, 1675, 1991
AcoI	YGGCCR	1 cut(s)	174
AcsI	RAATTY	3 cut(s)	394, 1892, 1931
AcyI	GRCGYC	2 cut(s)	1773, 1819
AfaI	GTAC	2 cut(s)	786, 1346
AfiI	CCNNNNNNNGG	3 cut(s)	479, 1210, 1678
AflIII	ACRYGT	1 cut(s)	292
AhdI	GACNNNNNGTC	1 cut(s)	1777
AjiI	CACGTC	1 cut(s)	623
AjnI	CCWGG	5 cut(s)	1014, 1585, 1671, 1692, 1842
AloI	GAACNNNNNNTCC	2 cut(s)	996, 1028
AluBI	AGCT	7 cut(s)	82, 691, 830, 869, 1502, 1738, 1880
AluI	AGCT	7 cut(s)	82, 691, 830, 869, 1502, 1738, 1880
Alw21I	GWGCWC	1 cut(s)	1740
Alw26I	GTCTC	2 cut(s)	842, 1077
AlwI	GGATC	4 cut(s)	1514, 1662, 1675, 1991
AlwNI	CAGNNNCTG	1 cut(s)	593
Ama87I	CYCGRG	2 cut(s)	1490, 1610
AoxI	GGCC	5 cut(s)	105, 174, 521, 630, 1970
ApeKI	GCWGC	6 cut(s)	133, 142, 145, 148, 593, 931
ApoI	RAATTY	3 cut(s)	394, 1892, 1931
ArsI	GACNNNNNNTTYG	2 cut(s)	607, 639
Asp700I	GAANNNNTTC	2 cut(s)	1005, 1935
AspLEI	GCGC	1 cut(s)	252
AspS9I	GGNCC	1 cut(s)	52
AsuC2I	CCSGG	1 cut(s)	1340
AsuHPI	GGTGA	3 cut(s)	151, 462, 980
AsuII	TTCGAA	1 cut(s)	752
AvaI	CYCGRG	2 cut(s)	1490, 1610
AvaII	GGWCC	1 cut(s)	52
BamHI	GGATCC	1 cut(s)	1667
BanII	GRGCYC	1 cut(s)	1740
BarI	GAAGNNNNNNTAC	2 cut(s)	1304, 1336
BbsI	GAAGAC	1 cut(s)	1822
Bbv12I	GWGCWC	1 cut(s)	1740
BbvI	GCAGC	6 cut(s)	145, 154, 157, 160, 580, 943
BccI	CCATC	8 cut(s)	263, 305, 316, 854, 965, 1139, 1906, 1919
BceAI	ACGGC	3 cut(s)	180, 639, 1926
BciT130I	CCWGG	5 cut(s)	1016, 1587, 1673, 1694, 1844
BclI	TGATCA	3 cut(s)	433, 1408, 1834
BcnI	CCSGG	1 cut(s)	1340
BcoDI	GTCTC	2 cut(s)	842, 1077
BfaI	CTAG	4 cut(s)	599, 1010, 1040, 1503
BfoI	RGCGCY	1 cut(s)	253
BfuAI	ACCTGC	2 cut(s)	1775, 1915
BglII	AGATCT	3 cut(s)	877, 1023, 1453
BisI	GCNGC	7 cut(s)	134, 143, 146, 149, 594, 929, 932
BlpI	GCTNAGC	1 cut(s)	1739
BlsI	GCNGC	7 cut(s)	135, 144, 147, 150, 595, 930, 933
Bme1390I	CCNGG	6 cut(s)	1016, 1340, 1587, 1673, 1694, 1844
Bme18I	GGWCC	1 cut(s)	52
BmeRI	GACNNNNNGTC	1 cut(s)	1777
BmeT110I	CYCGRG	2 cut(s)	1490, 1610
BmgBI	CACGTC	1 cut(s)	623
BmgT120I	GGNCC	1 cut(s)	52
BmiI	GGNNCC	2 cut(s)	1150, 1669
BmrFI	CCNGG	6 cut(s)	1016, 1340, 1587, 1673, 1694, 1844
BmsI	GCATC	5 cut(s)	581, 970, 1056, 1378, 1450
BoxI	GACNNNNGTC	1 cut(s)	1635
BpiI	GAAGAC	1 cut(s)	1822
BpmI	CTGGAG	2 cut(s)	113, 1118
Bpu1102I	GCTNAGC	1 cut(s)	1739
Bpu14I	TTCGAA	1 cut(s)	752
BpuEI	CTTGAG	1 cut(s)	1977
BpuMI	CCSGG	1 cut(s)	1340
BsaAI	YACGTR	2 cut(s)	581, 1118
BsaBI	GATNNNNATC	2 cut(s)	363, 1511
BsaHI	GRCGYC	2 cut(s)	1773, 1819
BsaI	GGTCTC	1 cut(s)	1077
BsaJI	CCNNGG	7 cut(s)	162, 1204, 1671, 1672, 1692, 1693, 1842
Bsc4I	CCNNNNNNNGG	3 cut(s)	479, 1210, 1678
Bse1I	ACTGG	3 cut(s)	46, 994, 1101
Bse3DI	GCAATG	1 cut(s)	817
Bse8I	GATNNNNATC	2 cut(s)	363, 1511
BseBI	CCWGG	5 cut(s)	1016, 1587, 1673, 1694, 1844
BseDI	CCNNGG	7 cut(s)	162, 1204, 1671, 1672, 1692, 1693, 1842
BseGI	GGATG	7 cut(s)	13, 18, 1024, 1150, 1663, 1688, 1898
BseJI	GATNNNNATC	2 cut(s)	363, 1511
BseLI	CCNNNNNNNGG	3 cut(s)	479, 1210, 1678
BseMI	GCAATG	1 cut(s)	817
BseMII	CTCAG	5 cut(s)	257, 480, 837, 1307, 1753
BseNI	ACTGG	3 cut(s)	46, 994, 1101
BseRI	GAGGAG	5 cut(s)	19, 28, 100, 617, 1073
BseXI	GCAGC	6 cut(s)	145, 154, 157, 160, 580, 943
BseYI	CCCAGC	1 cut(s)	1700
BsgI	GTGCAG	1 cut(s)	1408
BshFI	GGCC	5 cut(s)	107, 176, 523, 632, 1972
BsiHKAI	GWGCWC	1 cut(s)	1740
BsiHKCI	CYCGRG	2 cut(s)	1490, 1610
BsiSI	CCGG	1 cut(s)	1339
BslFI	GGGAC	2 cut(s)	1406, 1717
BslI	CCNNNNNNNGG	3 cut(s)	479, 1210, 1678
BsmAI	GTCTC	2 cut(s)	842, 1077
BsmFI	GGGAC	2 cut(s)	1406, 1717
BsmI	GAATGC	2 cut(s)	999, 1733
BsnI	GGCC	5 cut(s)	107, 176, 523, 632, 1972
Bso31I	GGTCTC	1 cut(s)	1077
BsoBI	CYCGRG	2 cut(s)	1490, 1610
Bsp119I	TTCGAA	1 cut(s)	752
Bsp1286I	GDGCHC	1 cut(s)	1740
Bsp1720I	GCTNAGC	1 cut(s)	1739
BspACI	CCGC	3 cut(s)	114, 550, 928
BspANI	GGCC	5 cut(s)	107, 176, 523, 632, 1972
BspCNI	CTCAG	5 cut(s)	256, 481, 838, 1306, 1752
BspLI	GGNNCC	2 cut(s)	1150, 1669
BspMI	ACCTGC	2 cut(s)	1775, 1915
BspPI	GGATC	4 cut(s)	1514, 1662, 1675, 1991
BspQI	GCTCTTC	1 cut(s)	559
BspT104I	TTCGAA	1 cut(s)	752
BspTNI	GGTCTC	1 cut(s)	1077
BsrBI	CCGCTC	2 cut(s)	552, 928
BsrDI	GCAATG	1 cut(s)	817
BsrI	ACTGG	3 cut(s)	46, 994, 1101
BssECI	CCNNGG	7 cut(s)	162, 1204, 1671, 1672, 1692, 1693, 1842
BssNI	GRCGYC	2 cut(s)	1773, 1819
BssT1I	CCWWGG	1 cut(s)	1204
Bst2UI	CCWGG	5 cut(s)	1016, 1587, 1673, 1694, 1844
Bst4CI	ACNGT	6 cut(s)	283, 337, 466, 589, 838, 1429
Bst6I	CTCTTC	2 cut(s)	559, 1299
BstACI	GRCGYC	2 cut(s)	1773, 1819
BstAPI	GCANNNNNTGC	1 cut(s)	590
BstBAI	YACGTR	2 cut(s)	581, 1118
BstBI	TTCGAA	1 cut(s)	752
BstC8I	GCNNGC	3 cut(s)	521, 867, 1045
BstDEI	CTNAG	9 cut(s)	243, 489, 846, 893, 912, 1293, 1432, 1640, 1739
BstDSI	CCRYGG	1 cut(s)	162
BstENI	CCTNNNNNAGG	1 cut(s)	1208
BstF5I	GGATG	7 cut(s)	13, 18, 1024, 1150, 1663, 1688, 1898
BstH2I	RGCGCY	1 cut(s)	253
BstHHI	GCGC	1 cut(s)	252
BstMAI	GTCTC	2 cut(s)	842, 1077
BstMWI	GCNNNNNNNGC	8 cut(s)	113, 122, 139, 142, 148, 173, 529, 590
BstNI	CCWGG	5 cut(s)	1016, 1587, 1673, 1694, 1844
BstNSI	RCATGY	4 cut(s)	16, 296, 1272, 1334
BstPAI	GACNNNNGTC	1 cut(s)	1635
BstSCI	CCNGG	6 cut(s)	1014, 1338, 1585, 1671, 1692, 1842
BstV1I	GCAGC	6 cut(s)	145, 154, 157, 160, 580, 943
BstV2I	GAAGAC	1 cut(s)	1822
BstX2I	RGATCY	5 cut(s)	877, 1023, 1453, 1667, 1996
BstXI	CCANNNNNNTGG	1 cut(s)	1700
BstYI	RGATCY	5 cut(s)	877, 1023, 1453, 1667, 1996
BsuRI	GGCC	5 cut(s)	107, 176, 523, 632, 1972
BtgI	CCRYGG	1 cut(s)	162
BtrI	CACGTC	1 cut(s)	623
BtsCI	GGATG	7 cut(s)	13, 18, 1024, 1150, 1663, 1688, 1898
BtsI	GCAGTG	2 cut(s)	309, 1450
BtsIMutI	CAGTG	6 cut(s)	309, 436, 1228, 1301, 1425, 1450
BveI	ACCTGC	2 cut(s)	1775, 1915
Cac8I	GCNNGC	3 cut(s)	521, 867, 1045
CaiI	CAGNNNCTG	1 cut(s)	593
CfoI	GCGC	1 cut(s)	252
Cfr13I	GGNCC	1 cut(s)	52
CseI	GACGC	1 cut(s)	1827
Csp6I	GTAC	2 cut(s)	785, 1345
CspCI	CAANNNNNGTGG	2 cut(s)	55, 90
CviAII	CATG	8 cut(s)	13, 293, 377, 852, 1269, 1280, 1331, 1832
CviQI	GTAC	2 cut(s)	785, 1345
DdeI	CTNAG	9 cut(s)	243, 489, 846, 893, 912, 1293, 1432, 1640, 1739
DraI	TTTAAA	1 cut(s)	1287
DriI	GACNNNNNGTC	1 cut(s)	1777
EaeI	YGGCCR	1 cut(s)	174
Eam1104I	CTCTTC	2 cut(s)	559, 1299
Eam1105I	GACNNNNNGTC	1 cut(s)	1777
EarI	CTCTTC	2 cut(s)	559, 1299
EciI	GGCGGA	1 cut(s)	103
Ecl136II	GAGCTC	1 cut(s)	1738
Eco130I	CCWWGG	1 cut(s)	1204
Eco147I	AGGCCT	3 cut(s)	107, 523, 632
Eco24I	GRGCYC	1 cut(s)	1740
Eco31I	GGTCTC	1 cut(s)	1077
Eco32I	GATATC	1 cut(s)	744
Eco47I	GGWCC	1 cut(s)	52
Eco53kI	GAGCTC	1 cut(s)	1738
Eco88I	CYCGRG	2 cut(s)	1490, 1610
EcoICRI	GAGCTC	1 cut(s)	1738
EcoNI	CCTNNNNNAGG	1 cut(s)	1208
EcoRI	GAATTC	1 cut(s)	394
EcoRII	CCWGG	5 cut(s)	1014, 1585, 1671, 1692, 1842
EcoRV	GATATC	1 cut(s)	744
EcoT14I	CCWWGG	1 cut(s)	1204
EcoT38I	GRGCYC	1 cut(s)	1740
ErhI	CCWWGG	1 cut(s)	1204
FaeI	CATG	8 cut(s)	16, 296, 380, 855, 1272, 1283, 1334, 1835
FalI	AAGNNNNNCTT	4 cut(s)	990, 1022, 1807, 1839
FaqI	GGGAC	2 cut(s)	1406, 1717
FatI	CATG	8 cut(s)	12, 292, 376, 851, 1268, 1279, 1330, 1831
FbaI	TGATCA	3 cut(s)	433, 1408, 1834
Fnu4HI	GCNGC	7 cut(s)	134, 143, 146, 149, 594, 929, 932
FokI	GGATG	7 cut(s)	5, 20, 1031, 1157, 1670, 1675, 1885
FriOI	GRGCYC	1 cut(s)	1740
Fsp4HI	GCNGC	7 cut(s)	134, 143, 146, 149, 594, 929, 932
FspBI	CTAG	4 cut(s)	599, 1010, 1040, 1503
GlaI	GCGC	1 cut(s)	251
GluI	GCNGC	7 cut(s)	134, 143, 146, 149, 594, 929, 932
GsaI	CCCAGC	1 cut(s)	1704
GsuI	CTGGAG	2 cut(s)	113, 1118
HaeII	RGCGCY	1 cut(s)	253
HaeIII	GGCC	5 cut(s)	107, 176, 523, 632, 1972
HapII	CCGG	1 cut(s)	1339
HgaI	GACGC	1 cut(s)	1827
HhaI	GCGC	1 cut(s)	252
Hin1I	GRCGYC	2 cut(s)	1773, 1819
Hin1II	CATG	8 cut(s)	16, 296, 380, 855, 1272, 1283, 1334, 1835
Hin6I	GCGC	1 cut(s)	250
HinP1I	GCGC	1 cut(s)	250
HincII	GTYRAC	1 cut(s)	1771
HindII	GTYRAC	1 cut(s)	1771
HindIII	AAGCTT	1 cut(s)	867
HinfI	GANTC	7 cut(s)	156, 197, 426, 646, 794, 1171, 1218
HpaII	CCGG	1 cut(s)	1339
HphI	GGTGA	3 cut(s)	151, 462, 980
Hpy166II	GTNNAC	3 cut(s)	1231, 1274, 1771
Hpy188III	TCNNGA	9 cut(s)	263, 287, 423, 599, 626, 1457, 1724, 1902, 1994
Hpy8I	GTNNAC	3 cut(s)	1231, 1274, 1771
Hpy99I	CGWCG	1 cut(s)	206
HpyAV	CCTTC	7 cut(s)	128, 192, 468, 661, 1096, 1101, 1759
HpyCH4III	ACNGT	6 cut(s)	283, 337, 466, 589, 838, 1429
HpyCH4IV	ACGT	4 cut(s)	580, 622, 1117, 1773
HpyCH4V	TGCA	9 cut(s)	572, 584, 997, 1268, 1334, 1369, 1425, 1443, 1619
HpyF10VI	GCNNNNNNNGC	8 cut(s)	113, 122, 139, 142, 148, 173, 529, 590
HpyF3I	CTNAG	9 cut(s)	243, 489, 846, 893, 912, 1293, 1432, 1640, 1739
HpySE526I	ACGT	4 cut(s)	580, 622, 1117, 1773
Hsp92I	GRCGYC	2 cut(s)	1773, 1819
Hsp92II	CATG	8 cut(s)	16, 296, 380, 855, 1272, 1283, 1334, 1835
HspAI	GCGC	1 cut(s)	250
Ksp22I	TGATCA	3 cut(s)	433, 1408, 1834
LguI	GCTCTTC	1 cut(s)	559
Lsp1109I	GCAGC	6 cut(s)	145, 154, 157, 160, 580, 943
LweI	GCATC	5 cut(s)	581, 970, 1056, 1378, 1450
MaeI	CTAG	4 cut(s)	599, 1010, 1040, 1503
MaeII	ACGT	4 cut(s)	580, 622, 1117, 1773
MaeIII	GTNAC	4 cut(s)	451, 1319, 1485, 1624
MbiI	CCGCTC	2 cut(s)	552, 928
MboII	GAAGA	9 cut(s)	278, 546, 869, 958, 1013, 1316, 1321, 1573, 1827
MflI	RGATCY	5 cut(s)	877, 1023, 1453, 1667, 1996
MhlI	GDGCHC	1 cut(s)	1740
MluCI	AATT	9 cut(s)	274, 394, 399, 671, 899, 1162, 1869, 1892, 1931
MlyI	GAGTC	2 cut(s)	435, 788
MmeI	TCCRAC	5 cut(s)	30, 57, 66, 225, 1645
MroXI	GAANNNNTTC	2 cut(s)	1005, 1935
MseI	TTAA	6 cut(s)	779, 1158, 1196, 1286, 1470, 1875
MslI	CAYNNNNRTG	3 cut(s)	381, 1185, 1260
MspI	CCGG	1 cut(s)	1339
MspR9I	CCNGG	6 cut(s)	1016, 1340, 1587, 1673, 1694, 1844
Mva1269I	GAATGC	2 cut(s)	999, 1733
MvaI	CCWGG	5 cut(s)	1016, 1587, 1673, 1694, 1844
MwoI	GCNNNNNNNGC	8 cut(s)	113, 122, 139, 142, 148, 173, 529, 590
NciI	CCSGG	1 cut(s)	1340
NlaIII	CATG	8 cut(s)	16, 296, 380, 855, 1272, 1283, 1334, 1835
NlaIV	GGNNCC	2 cut(s)	1150, 1669
NmeAIII	GCCGAG	2 cut(s)	177, 202
NmuCI	GTSAC	1 cut(s)	1624
NspI	RCATGY	4 cut(s)	16, 296, 1272, 1334
NspV	TTCGAA	1 cut(s)	752
PasI	CCCWGGG	2 cut(s)	1672, 1693
PceI	AGGCCT	3 cut(s)	107, 523, 632
PciI	ACATGT	1 cut(s)	292
PciSI	GCTCTTC	1 cut(s)	559
PctI	GAATGC	2 cut(s)	999, 1733
PdmI	GAANNNNTTC	2 cut(s)	1005, 1935
PfeI	GAWTC	5 cut(s)	156, 197, 646, 1171, 1218
PflMI	CCANNNNNTGG	1 cut(s)	1678
PfoI	TCCNGGA	2 cut(s)	1014, 1338
PkrI	GCNGC	7 cut(s)	135, 144, 147, 150, 595, 930, 933
PleI	GAGTC	2 cut(s)	434, 788
PpsI	GAGTC	2 cut(s)	434, 788
Ppu21I	YACGTR	2 cut(s)	581, 1118
PscI	ACATGT	1 cut(s)	292
PshAI	GACNNNNGTC	1 cut(s)	1635
Psp124BI	GAGCTC	1 cut(s)	1740
Psp6I	CCWGG	5 cut(s)	1014, 1585, 1671, 1692, 1842
PspFI	CCCAGC	1 cut(s)	1700
PspGI	CCWGG	5 cut(s)	1014, 1585, 1671, 1692, 1842
PspN4I	GGNNCC	2 cut(s)	1150, 1669
PspPI	GGNCC	1 cut(s)	52
PstNI	CAGNNNCTG	1 cut(s)	593
PsuI	RGATCY	5 cut(s)	877, 1023, 1453, 1667, 1996
RsaI	GTAC	2 cut(s)	786, 1346
RsaNI	GTAC	2 cut(s)	785, 1345
RseI	CAYNNNNRTG	3 cut(s)	381, 1185, 1260
SacI	GAGCTC	1 cut(s)	1740
SapI	GCTCTTC	1 cut(s)	559
SaqAI	TTAA	6 cut(s)	779, 1158, 1196, 1286, 1470, 1875
SatI	GCNGC	7 cut(s)	134, 143, 146, 149, 594, 929, 932
Sau96I	GGNCC	1 cut(s)	52
SchI	GAGTC	2 cut(s)	435, 788
ScrFI	CCNGG	6 cut(s)	1016, 1340, 1587, 1673, 1694, 1844
SduI	GDGCHC	1 cut(s)	1740
SfaNI	GCATC	5 cut(s)	581, 970, 1056, 1378, 1450
SfuI	TTCGAA	1 cut(s)	752
SinI	GGWCC	1 cut(s)	52
SmiMI	CAYNNNNRTG	3 cut(s)	381, 1185, 1260
SmlI	CTYRAG	1 cut(s)	1992
SmoI	CTYRAG	1 cut(s)	1992
Sse9I	AATT	9 cut(s)	274, 394, 399, 671, 899, 1162, 1869, 1892, 1931
SseBI	AGGCCT	3 cut(s)	107, 523, 632
SsiI	CCGC	3 cut(s)	114, 550, 928
SspMI	CTAG	4 cut(s)	599, 1010, 1040, 1503
SstI	GAGCTC	1 cut(s)	1740
StuI	AGGCCT	3 cut(s)	107, 523, 632
StyD4I	CCNGG	6 cut(s)	1014, 1338, 1585, 1671, 1692, 1842
StyI	CCWWGG	1 cut(s)	1204
TaaI	ACNGT	6 cut(s)	283, 337, 466, 589, 838, 1429
TaiI	ACGT	4 cut(s)	583, 625, 1120, 1776
TaqI	TCGA	8 cut(s)	84, 191, 461, 752, 1384, 1734, 1763, 1882
TaqII	GACCGA	1 cut(s)	1101
TasI	AATT	9 cut(s)	274, 394, 399, 671, 899, 1162, 1869, 1892, 1931
TatI	WGTACW	1 cut(s)	1344
TauI	GCSGC	1 cut(s)	931
TfiI	GAWTC	5 cut(s)	156, 197, 646, 1171, 1218
Tru1I	TTAA	6 cut(s)	779, 1158, 1196, 1286, 1470, 1875
Tru9I	TTAA	6 cut(s)	779, 1158, 1196, 1286, 1470, 1875
TscAI	CASTG	6 cut(s)	309, 436, 1228, 1301, 1432, 1450
TseFI	GTSAC	1 cut(s)	1624
TseI	GCWGC	6 cut(s)	133, 142, 145, 148, 593, 931
Tsp45I	GTSAC	1 cut(s)	1624
TspDTI	ATGAA	6 cut(s)	546, 719, 1107, 1139, 1268, 1935
TspRI	CASTG	6 cut(s)	309, 436, 1228, 1301, 1432, 1450
Van91I	CCANNNNNTGG	1 cut(s)	1678
VpaK11BI	GGWCC	1 cut(s)	52
XagI	CCTNNNNNAGG	1 cut(s)	1208
XapI	RAATTY	3 cut(s)	394, 1892, 1931
XbaI	TCTAGA	1 cut(s)	598
XceI	RCATGY	4 cut(s)	16, 296, 1272, 1334
XcmI	CCANNNNNNNNNTGG	2 cut(s)	169, 1808
XmnI	GAANNNNTTC	2 cut(s)	1005, 1935
XspI	CTAG	4 cut(s)	599, 1010, 1040, 1503
ZraI	GACGTC	1 cut(s)	1774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.