Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0012973

Physical Location & Seq

Genomic Coordinates Reverse (-)

46167572 .. 46175492

Gene Structure 7921 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00032310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGTCGGATGACATGCTGCTCCATTTCTCTTCCAATTCGTCCAACCAGTCGGACCACTCTCTTCCCACCAAGATTGCCAAGCTCGAAGCTCGCATGGTCGGCAAGGCTTCCTCCGCTCCTCCTCCTCCTGCTCAGCCTCAGTCTGCTTGGTCCTCCTCCCTCTCGTCTGCCCCCAAATTCGTGACGGCTGACTTGTCCGCCCAGCCTTCTACTTCCAGTGATTCCGATGATGATAATGGAGGGGAGTTTTTAATACAAGCCAATACTCAGAAGCGCCAGAAACTTCAACAAGATGTTACTTCAACAACTATCTTTGAACATGTTGAGGCAGTGGCTGATGTGAGGCAAATGGTTGTAGATACCAAGGTAAATTCTGATGTAAATAGAAAAAAACATGGTCGTGGTAGGGCCAATTCTGGCTTAGGTCGGGGTCGCGGCTCAAGAGTTCATGATCAGACAAAATCATTAATTTCTCCTCCAACAGTTTCCCCCCCAAATGGTGAGCTTGAGAACTCATACAACAAGGATAGCAGGTCTAAAGATCAGCTTCGTAATGACAACAGCTCTTCCTTAGATGAGGAGGTTGCGACTTTACGTGTAAAAGTTTCTGCACTAGAGGAGGATTTGCGTAAATCACGACAAGAGGCCTCTGATCATCAGAATCTTTTCCGCCAATTAGAGAAAGAGTTAAAAGAAGTAAAAGATTATGAACAGAAAATGAAGCCTAAGAGAACAAAAGTAATCTCCGATCTGCTGATTTCTGTTTCAAAAGCAGAGAGGCAGGAAGCAAGGTTGAAAGTACGACAGGACTCCTTGAGGCTTGGCCATGTGGGTGTAATCAGAGCTGGAACTGTAATATCTGAGACATGGGAAGATGGGCAAGCCTTGAAGGATCTTAATGCTCATCTTAGACAATTATTAGAAACTAAGGAGGCTGTTGAGAGGCAGAGAAAGTCGTTGAAAAAAAGGCAAACTGATAAGAGTGATGCAGTTGATGCTGAAACTGGAATACAAGAAGAATCTCTAATCCAGGATGAGATCTATAAATCCCGTCTAGCGAGCATCAAACGAGAGGAGGAAGTTTTACTTCGTGAAAGGGATCGCTATGAACTGGAAAAGGGAAGGCTTATACGTGAAATGAAACGCATACGTGATGAGGATGGTTCCCGTTTTAACAATTTTCAGATTCTCAACCACAGATATGCCCTCTTAAATCTTCTTGGCAAAGGAGGATTCAGTGAAGTTTACAAGGCATTTGACTTGGTAGAGCACAGATATGTTGCATGTAAACTTCATGGTTTAAATGCTCAGTGGAGTGAGGAGAAGAAGCAGAGTTACATAAGGCACGCAATTCGGGAATACAATATTCACAAAACCTTGGTGCATCATCACATTGTCCGGCTTTGGGACATTTTTGAGATCGATCAGAATACATTCTGCACCATCTTGGAGTACTGTAGTGGGAAAGATCTTGACGCTGTTCTCAAAGCAACACCTGTATTGCCTGAGAGGGAAGCTAGGATCATTATCTTTCAGATATTTCAAGGCCTTATATACTTGAACAAGAGAACACAGAAGATTATACATTATGATTTGAAGCCTGGAAATGTTCTATTTGATGAACTTGGTGTTGCGAAAGTGACTGATTTTGGTCTCAGCAAGATTGTGGAAGATGATGTTGGATCCCAGGGTATGGAACTTACGTCCCAGGGTGCTGGAACTTATTGGTATCTACCTCCTGAATGCTTTGAGCTCAGCAGGACACCTCTTATCTCATCAAAGGTTGATGTTTGGTCAGCTGGTATTCTATTGTACCAAATGTTATTTGGCAGACGTCCTTTTGGGCATGACCAGACCCAGGAACGAATACTGCGGGAAGACACAATTATCAAAGCACGAAAAGTTGAGTTTCCTTCTAGACCATCCATCTCAAATGAGGCAAAGGAATTTATTCGGCGTTGTCTAACATATAATCAGGCTGAGAGGCCAGATGTATTGGCAATAGCTCAAGATCCATTTCTGACATACTCAAAAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0060147 GO:0060255 GO:0060966 GO:0060968 GO:0065007 GO:0071704 GO:0140096 GO:1900368 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

679

Amino Acids

77.47

Weight (kDa)

8.6

Isoelectric Point (pI)

45.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	401 - 674	7.7e-59	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	404 - 670	9.5e-32	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20930
fragaria_vesca	FvH4_3g26940
malus_domestica	MD03G1161400.v1.1 MD11G1177500.v1.1
prunus_persica	Prupe.6G149600_v2.0.a1 Prupe.6G149600_v2.0.a1 Prupe.6G149600_v2.0.a1
pyrus_communis	pycom11g15140
rosa_chinensis	RchiOBHm_Chr5g0049381
rosa_laevigata	RLG00000034615
rosa_multiflora	Rmu_sc0014052.1_g000012
rosa_roxburghii	Rroxscaffold_1G00032310
rosa_rugosa	Rorug05G0248100
rosa_samantha	Rh5AG324500 Rh5BG335600 Rh5CG360300 Rh5DG347900
rosa_wichuraiana	Rw5G030660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1837
Acc36I	ACCTGC	1 cut(s)	522
AccB7I	CCANNNNNTGG	1 cut(s)	1693
AccBSI	CCGCTC	1 cut(s)	116
AccII	CGCG	1 cut(s)	435
AciI	CCGC	5 cut(s)	114, 198, 435, 670, 1873
AclWI	GGATC	6 cut(s)	900, 1107, 1529, 1677, 1690, 2006
AcoI	YGGCCR	1 cut(s)	823
AcsI	RAATTY	3 cut(s)	176, 370, 1946
AcyI	GRCGYC	1 cut(s)	1834
AfaI	GTAC	3 cut(s)	801, 1454, 1814
AfiI	CCNNNNNNNGG	4 cut(s)	497, 1405, 1693, 1858
AflIII	ACRYGT	2 cut(s)	319, 595
AjnI	CCWGG	5 cut(s)	1029, 1600, 1686, 1707, 1857
AjuI	GAANNNNNNNTTGG	2 cut(s)	1662, 1694
Alw21I	GWGCWC	2 cut(s)	1272, 1755
Alw26I	GTCTC	2 cut(s)	857, 1658
AlwI	GGATC	6 cut(s)	900, 1107, 1529, 1677, 1690, 2006
AlwNI	CAGNNNCTG	1 cut(s)	335
AoxI	GGCC	5 cut(s)	408, 645, 823, 1546, 1985
ApeKI	GCWGC	1 cut(s)	16
ApoI	RAATTY	3 cut(s)	176, 370, 1946
AseI	ATTAAT	1 cut(s)	467
Asp700I	GAANNNNTTC	3 cut(s)	665, 1433, 1950
AspLEI	GCGC	1 cut(s)	276
AspS9I	GGNCC	3 cut(s)	52, 150, 408
AsuHPI	GGTGA	1 cut(s)	512
AvaII	GGWCC	2 cut(s)	52, 150
BaeI	ACNNNNGTAYC	2 cut(s)	351, 384
BalI	TGGCCA	1 cut(s)	825
BamHI	GGATCC	1 cut(s)	1682
BanII	GRGCYC	1 cut(s)	1755
BarI	GAAGNNNNNNTAC	2 cut(s)	1319, 1351
BbsI	GAAGAC	1 cut(s)	1884
Bbv12I	GWGCWC	2 cut(s)	1272, 1755
BbvI	GCAGC	1 cut(s)	3
BccI	CCATC	5 cut(s)	869, 1154, 1451, 1930, 1934
BceAI	ACGGC	1 cut(s)	201
BcgI	CGANNNNNNTGC	2 cut(s)	1334, 1368
BciT130I	CCWGG	5 cut(s)	1031, 1602, 1688, 1709, 1859
BclI	TGATCA	2 cut(s)	451, 652
BcoDI	GTCTC	2 cut(s)	857, 1658
BfaI	CTAG	4 cut(s)	614, 1055, 1518, 1917
BfmI	CTRYAG	1 cut(s)	1456
BfoI	RGCGCY	1 cut(s)	277
BfuAI	ACCTGC	1 cut(s)	522
BglII	AGATCT	2 cut(s)	1038, 1468
BisI	GCNGC	2 cut(s)	17, 436
BlpI	GCTNAGC	2 cut(s)	132, 1754
BlsI	GCNGC	2 cut(s)	18, 437
BmcAI	AGTACT	1 cut(s)	1454
Bme1390I	CCNGG	5 cut(s)	1031, 1602, 1688, 1709, 1859
Bme18I	GGWCC	2 cut(s)	52, 150
BmgT120I	GGNCC	3 cut(s)	52, 150, 408
BmiI	GGNNCC	2 cut(s)	1165, 1684
BmrFI	CCNGG	5 cut(s)	1031, 1602, 1688, 1709, 1859
BmsI	GCATC	4 cut(s)	976, 985, 1071, 1393
BpiI	GAAGAC	1 cut(s)	1884
Bpu10I	CCTNAGC	1 cut(s)	421
Bpu1102I	GCTNAGC	2 cut(s)	132, 1754
BpuEI	CTTGAG	4 cut(s)	424, 527, 835, 1992
Bsa29I	ATCGAT	1 cut(s)	1422
BsaAI	YACGTR	3 cut(s)	596, 1133, 1151
BsaBI	GATNNNNATC	1 cut(s)	1526
BsaHI	GRCGYC	1 cut(s)	1834
BsaI	GGTCTC	1 cut(s)	1658
BsaJI	CCNNGG	7 cut(s)	363, 1377, 1686, 1687, 1707, 1708, 1857
Bsc4I	CCNNNNNNNGG	4 cut(s)	497, 1405, 1693, 1858
Bse1I	ACTGG	4 cut(s)	46, 216, 1009, 1116
Bse8I	GATNNNNATC	1 cut(s)	1526
BseBI	CCWGG	5 cut(s)	1031, 1602, 1688, 1709, 1859
BseCI	ATCGAT	1 cut(s)	1422
BseDI	CCNNGG	7 cut(s)	363, 1377, 1686, 1687, 1707, 1708, 1857
BseGI	GGATG	4 cut(s)	13, 1039, 1165, 1922
BseJI	GATNNNNATC	1 cut(s)	1526
BseLI	CCNNNNNNNGG	4 cut(s)	497, 1405, 1693, 1858
BseMII	CTCAG	9 cut(s)	146, 152, 281, 852, 1322, 1497, 1669, 1768, 1971
BseNI	ACTGG	4 cut(s)	46, 216, 1009, 1116
BseRI	GAGGAG	9 cut(s)	108, 111, 114, 145, 465, 593, 632, 1088, 1334
BseXI	GCAGC	1 cut(s)	3
BseYI	CCCAGC	1 cut(s)	201
BsgI	GTGCAG	2 cut(s)	594, 1423
Bsh1236I	CGCG	1 cut(s)	435
BshFI	GGCC	5 cut(s)	410, 647, 825, 1548, 1987
BshVI	ATCGAT	1 cut(s)	1422
BsiHKAI	GWGCWC	2 cut(s)	1272, 1755
BsiSI	CCGG	1 cut(s)	1399
BslFI	GGGAC	2 cut(s)	1421, 1690
BslI	CCNNNNNNNGG	4 cut(s)	497, 1405, 1693, 1858
BsmAI	GTCTC	2 cut(s)	857, 1658
BsmFI	GGGAC	2 cut(s)	1421, 1690
BsmI	GAATGC	1 cut(s)	1748
BsnI	GGCC	5 cut(s)	410, 647, 825, 1548, 1987
Bso31I	GGTCTC	1 cut(s)	1658
Bsp1286I	GDGCHC	2 cut(s)	1272, 1755
Bsp1720I	GCTNAGC	2 cut(s)	132, 1754
BspACI	CCGC	5 cut(s)	114, 198, 435, 670, 1873
BspANI	GGCC	5 cut(s)	410, 647, 825, 1548, 1987
BspCNI	CTCAG	9 cut(s)	145, 151, 280, 853, 1321, 1498, 1668, 1767, 1972
BspDI	ATCGAT	1 cut(s)	1422
BspFNI	CGCG	1 cut(s)	435
BspHI	TCATGA	1 cut(s)	448
BspLI	GGNNCC	2 cut(s)	1165, 1684
BspMI	ACCTGC	1 cut(s)	522
BspPI	GGATC	6 cut(s)	900, 1107, 1529, 1677, 1690, 2006
BspQI	GCTCTTC	1 cut(s)	571
BspTNI	GGTCTC	1 cut(s)	1658
BsrBI	CCGCTC	1 cut(s)	116
BsrI	ACTGG	4 cut(s)	46, 216, 1009, 1116
BssECI	CCNNGG	7 cut(s)	363, 1377, 1686, 1687, 1707, 1708, 1857
BssNI	GRCGYC	1 cut(s)	1834
BssT1I	CCWWGG	2 cut(s)	363, 1377
Bst2UI	CCWGG	5 cut(s)	1031, 1602, 1688, 1709, 1859
Bst4CI	ACNGT	3 cut(s)	484, 853, 1457
Bst6I	CTCTTC	3 cut(s)	34, 66, 571
BstACI	GRCGYC	1 cut(s)	1834
BstAPI	GCANNNNNTGC	1 cut(s)	995
BstBAI	YACGTR	3 cut(s)	596, 1133, 1151
BstC8I	GCNNGC	4 cut(s)	91, 882, 1060, 1347
BstF5I	GGATG	4 cut(s)	13, 1039, 1165, 1922
BstFNI	CGCG	1 cut(s)	435
BstH2I	RGCGCY	1 cut(s)	277
BstHHI	GCGC	1 cut(s)	276
BstMAI	GTCTC	2 cut(s)	857, 1658
BstMWI	GCNNNNNNNGC	3 cut(s)	99, 113, 995
BstNI	CCWGG	5 cut(s)	1031, 1602, 1688, 1709, 1859
BstNSI	RCATGY	3 cut(s)	16, 323, 1287
BstSCI	CCNGG	5 cut(s)	1029, 1600, 1686, 1707, 1857
BstSFI	CTRYAG	1 cut(s)	1456
BstUI	CGCG	1 cut(s)	435
BstV1I	GCAGC	1 cut(s)	3
BstV2I	GAAGAC	1 cut(s)	1884
BstX2I	RGATCY	5 cut(s)	892, 1038, 1468, 1682, 2011
BstXI	CCANNNNNNTGG	1 cut(s)	1715
BstYI	RGATCY	5 cut(s)	892, 1038, 1468, 1682, 2011
Bsu15I	ATCGAT	1 cut(s)	1422
BsuRI	GGCC	5 cut(s)	410, 647, 825, 1548, 1987
BsuTUI	ATCGAT	1 cut(s)	1422
BtsCI	GGATG	4 cut(s)	13, 1039, 1165, 1922
BtsI	GCAGTG	1 cut(s)	336
BtsIMutI	CAGTG	4 cut(s)	223, 336, 1243, 1316
BveI	ACCTGC	1 cut(s)	522
Cac8I	GCNNGC	4 cut(s)	91, 882, 1060, 1347
CaiI	CAGNNNCTG	1 cut(s)	335
CciI	TCATGA	1 cut(s)	448
CfoI	GCGC	1 cut(s)	276
Cfr13I	GGNCC	3 cut(s)	52, 150, 408
ClaI	ATCGAT	1 cut(s)	1422
CseI	GACGC	1 cut(s)	1484
Csp6I	GTAC	3 cut(s)	800, 1453, 1813
CspCI	CAANNNNNGTGG	2 cut(s)	55, 90
CviQI	GTAC	3 cut(s)	800, 1453, 1813
DraI	TTTAAA	1 cut(s)	1302
EaeI	YGGCCR	1 cut(s)	823
Eam1104I	CTCTTC	3 cut(s)	34, 66, 571
EarI	CTCTTC	3 cut(s)	34, 66, 571
EciI	GGCGGA	2 cut(s)	187, 659
Ecl136II	GAGCTC	1 cut(s)	1753
Eco130I	CCWWGG	2 cut(s)	363, 1377
Eco147I	AGGCCT	2 cut(s)	647, 1548
Eco24I	GRGCYC	1 cut(s)	1755
Eco31I	GGTCTC	1 cut(s)	1658
Eco47I	GGWCC	2 cut(s)	52, 150
Eco53kI	GAGCTC	1 cut(s)	1753
EcoICRI	GAGCTC	1 cut(s)	1753
EcoRII	CCWGG	5 cut(s)	1029, 1600, 1686, 1707, 1857
EcoT14I	CCWWGG	2 cut(s)	363, 1377
EcoT38I	GRGCYC	1 cut(s)	1755
ErhI	CCWWGG	2 cut(s)	363, 1377
FalI	AAGNNNNNCTT	4 cut(s)	531, 563, 1071, 1103
FaqI	GGGAC	2 cut(s)	1421, 1690
FauI	CCCGC	1 cut(s)	1866
FbaI	TGATCA	2 cut(s)	451, 652
Fnu4HI	GCNGC	2 cut(s)	17, 436
FokI	GGATG	4 cut(s)	20, 1046, 1172, 1909
FriOI	GRGCYC	1 cut(s)	1755
Fsp4HI	GCNGC	2 cut(s)	17, 436
FspBI	CTAG	4 cut(s)	614, 1055, 1518, 1917
GlaI	GCGC	1 cut(s)	275
GluI	GCNGC	2 cut(s)	17, 436
GsaI	CCCAGC	1 cut(s)	205
HaeII	RGCGCY	1 cut(s)	277
HaeIII	GGCC	5 cut(s)	410, 647, 825, 1548, 1987
HapII	CCGG	1 cut(s)	1399
HgaI	GACGC	1 cut(s)	1484
HhaI	GCGC	1 cut(s)	276
Hin1I	GRCGYC	1 cut(s)	1834
Hin6I	GCGC	1 cut(s)	274
HinP1I	GCGC	1 cut(s)	274
HinfI	GANTC	6 cut(s)	221, 661, 809, 1019, 1186, 1233
HpaII	CCGG	1 cut(s)	1399
HphI	GGTGA	1 cut(s)	512
Hpy166II	GTNNAC	2 cut(s)	1246, 1289
Hpy8I	GTNNAC	2 cut(s)	1246, 1289
HpyAV	CCTTC	4 cut(s)	216, 883, 1116, 1923
HpyCH4III	ACNGT	3 cut(s)	484, 853, 1457
HpyCH4IV	ACGT	5 cut(s)	595, 1132, 1150, 1703, 1834
HpyCH4V	TGCA	5 cut(s)	611, 989, 1283, 1384, 1440
HpyF10VI	GCNNNNNNNGC	3 cut(s)	99, 113, 995
HpySE526I	ACGT	5 cut(s)	595, 1132, 1150, 1703, 1834
Hsp92I	GRCGYC	1 cut(s)	1834
HspAI	GCGC	1 cut(s)	274
Ksp22I	TGATCA	2 cut(s)	451, 652
LguI	GCTCTTC	1 cut(s)	571
LmnI	GCTCC	2 cut(s)	24, 121
Lsp1109I	GCAGC	1 cut(s)	3
LweI	GCATC	4 cut(s)	976, 985, 1071, 1393
MaeI	CTAG	4 cut(s)	614, 1055, 1518, 1917
MaeII	ACGT	5 cut(s)	595, 1132, 1150, 1703, 1834
MaeIII	GTNAC	4 cut(s)	181, 295, 1334, 1639
MbiI	CCGCTC	1 cut(s)	116
MflI	RGATCY	5 cut(s)	892, 1038, 1468, 1682, 2011
MhlI	GDGCHC	2 cut(s)	1272, 1755
MlsI	TGGCCA	1 cut(s)	825
MluNI	TGGCCA	1 cut(s)	825
MlyI	GAGTC	1 cut(s)	803
MmeI	TCCRAC	4 cut(s)	30, 66, 503, 1660
Mox20I	TGGCCA	1 cut(s)	825
MroXI	GAANNNNTTC	3 cut(s)	665, 1433, 1950
MscI	TGGCCA	1 cut(s)	825
MseI	TTAA	7 cut(s)	251, 467, 689, 897, 1173, 1211, 1301
MslI	CAYNNNNRTG	4 cut(s)	399, 831, 1200, 1275
Msp20I	TGGCCA	1 cut(s)	825
MspA1I	CMGCKG	1 cut(s)	1799
MspI	CCGG	1 cut(s)	1399
MspR9I	CCNGG	5 cut(s)	1031, 1602, 1688, 1709, 1859
Mva1269I	GAATGC	1 cut(s)	1748
MvaI	CCWGG	5 cut(s)	1031, 1602, 1688, 1709, 1859
MvnI	CGCG	1 cut(s)	435
MwoI	GCNNNNNNNGC	3 cut(s)	99, 113, 995
NlaIV	GGNNCC	2 cut(s)	1165, 1684
NmuCI	GTSAC	2 cut(s)	181, 1639
NspI	RCATGY	3 cut(s)	16, 323, 1287
PagI	TCATGA	1 cut(s)	448
PasI	CCCWGGG	2 cut(s)	1687, 1708
PceI	AGGCCT	2 cut(s)	647, 1548
PciI	ACATGT	1 cut(s)	319
PciSI	GCTCTTC	1 cut(s)	571
PctI	GAATGC	1 cut(s)	1748
PdmI	GAANNNNTTC	3 cut(s)	665, 1433, 1950
PfeI	GAWTC	5 cut(s)	221, 661, 1019, 1186, 1233
PflMI	CCANNNNNTGG	1 cut(s)	1693
PfoI	TCCNGGA	1 cut(s)	1029
PkrI	GCNGC	2 cut(s)	18, 437
PleI	GAGTC	1 cut(s)	803
PpsI	GAGTC	1 cut(s)	803
Ppu21I	YACGTR	3 cut(s)	596, 1133, 1151
PscI	ACATGT	1 cut(s)	319
PshBI	ATTAAT	1 cut(s)	467
Psp124BI	GAGCTC	1 cut(s)	1755
Psp6I	CCWGG	5 cut(s)	1029, 1600, 1686, 1707, 1857
PspFI	CCCAGC	1 cut(s)	201
PspGI	CCWGG	5 cut(s)	1029, 1600, 1686, 1707, 1857
PspN4I	GGNNCC	2 cut(s)	1165, 1684
PspPI	GGNCC	3 cut(s)	52, 150, 408
PstNI	CAGNNNCTG	1 cut(s)	335
PsuI	RGATCY	5 cut(s)	892, 1038, 1468, 1682, 2011
PvuII	CAGCTG	1 cut(s)	1799
RsaI	GTAC	3 cut(s)	801, 1454, 1814
RsaNI	GTAC	3 cut(s)	800, 1453, 1813
RseI	CAYNNNNRTG	4 cut(s)	399, 831, 1200, 1275
SacI	GAGCTC	1 cut(s)	1755
SapI	GCTCTTC	1 cut(s)	571
SaqAI	TTAA	7 cut(s)	251, 467, 689, 897, 1173, 1211, 1301
SatI	GCNGC	2 cut(s)	17, 436
Sau96I	GGNCC	3 cut(s)	52, 150, 408
ScaI	AGTACT	1 cut(s)	1454
SchI	GAGTC	1 cut(s)	803
ScrFI	CCNGG	5 cut(s)	1031, 1602, 1688, 1709, 1859
SduI	GDGCHC	2 cut(s)	1272, 1755
SfaNI	GCATC	4 cut(s)	976, 985, 1071, 1393
SfcI	CTRYAG	1 cut(s)	1456
SinI	GGWCC	2 cut(s)	52, 150
SmiMI	CAYNNNNRTG	4 cut(s)	399, 831, 1200, 1275
SmlI	CTYRAG	4 cut(s)	439, 506, 814, 2007
SmoI	CTYRAG	4 cut(s)	439, 506, 814, 2007
SseBI	AGGCCT	2 cut(s)	647, 1548
SsiI	CCGC	5 cut(s)	114, 198, 435, 670, 1873
SspI	AATATT	1 cut(s)	1366
SspMI	CTAG	4 cut(s)	614, 1055, 1518, 1917
SstI	GAGCTC	1 cut(s)	1755
StuI	AGGCCT	2 cut(s)	647, 1548
StyD4I	CCNGG	5 cut(s)	1029, 1600, 1686, 1707, 1857
StyI	CCWWGG	2 cut(s)	363, 1377
TaaI	ACNGT	3 cut(s)	484, 853, 1457
TaiI	ACGT	5 cut(s)	598, 1135, 1153, 1706, 1837
TaqI	TCGA	2 cut(s)	84, 1422
TatI	WGTACW	1 cut(s)	1452
TauI	GCSGC	1 cut(s)	438
TfiI	GAWTC	5 cut(s)	221, 661, 1019, 1186, 1233
Tru1I	TTAA	7 cut(s)	251, 467, 689, 897, 1173, 1211, 1301
Tru9I	TTAA	7 cut(s)	251, 467, 689, 897, 1173, 1211, 1301
TscAI	CASTG	4 cut(s)	223, 336, 1243, 1316
TseFI	GTSAC	2 cut(s)	181, 1639
TseI	GCWGC	1 cut(s)	16
Tsp45I	GTSAC	2 cut(s)	181, 1639
TspDTI	ATGAA	7 cut(s)	437, 723, 734, 1122, 1154, 1283, 1635
TspRI	CASTG	4 cut(s)	223, 336, 1243, 1316
Van91I	CCANNNNNTGG	1 cut(s)	1693
VpaK11BI	GGWCC	2 cut(s)	52, 150
VspI	ATTAAT	1 cut(s)	467
XapI	RAATTY	3 cut(s)	176, 370, 1946
XbaI	TCTAGA	1 cut(s)	1916
XceI	RCATGY	3 cut(s)	16, 323, 1287
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1823
XmnI	GAANNNNTTC	3 cut(s)	665, 1433, 1950
XspI	CTAG	4 cut(s)	614, 1055, 1518, 1917
ZraI	GACGTC	1 cut(s)	1835
ZrmI	AGTACT	1 cut(s)	1454

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.