MD03G1170700.v1.1

Belongs to the hexokinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0018755
Physical Location & Seq
Reverse (-)
23150311 .. 23156437
6127 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1170700.v1.1.491

Sequence Viewer

Length: 1530 bp
ATGGGGAAGGTGGCGGTGGGATTGGCGGTTGGGGTGGCGGTGGCGGCGTGTGCGGTGGCGACGATGGTGGTGGGTCGGAGGATAAGGAGCAGAAGGAAGTGGGGAAGAGTGTTTGGGGTCTTGAAGGACCTGGAGCTAAGCTGTGAAACCACAGTGGGGAGGTTGAGGCAGGTGGTGGACGCCATGGCGGTGGAGATGCACGCTGGCTTGGCCTCTGACGGTGGCTCCAAGCTCAAAATGCTGCTCACTTTTGTGGATAAACTACCCGATGGGAGCGAAAAAGGAACTTATTATGCACTAGATCTTGGCGGTACTAACTTTAGGGTCTTGCGGGTTAAGCTTGGAGGTACAAGGTCCTCTACCTTGGAACATGATGTACATCAACAAGATATTCCTCAAAATTTGATGACTGGGACAAGCGAGGTTCTATTTGATTTTATTGCTTCATCAGTAAAGGAGTTTGTTGAAAGAGAAACAGACGATTCTAAGCTTTCGCTGGACAGAAGAAGGGAGCTTGGATTTATGTTCTCTTTTCCAGTGAAACAAACCTCTGTTTCATCAGGCACTTTATTAAAATGGACAAAAGGGTTTGCGATTGAAGACATGATTGGAAAAGAGGTTGCAGGATGCTTGGAGAAAGCAATGACCAAGAAGGGTCTAAATATGAGTGTGGCAGCACTTGTCAATGACTCTGTGGGAACATTATCACTTGGACATTATCACGACACAGACACTGTTGCTGCAGTAATAATTGGAACTGGCACTAATGCCTGTTATTTGGAAAGGACAGATGCTATAATTAAGTCTCAAGGACTACTTACAGCATCTGGAGGCATGGTTGTCAATATGGAATGGGGAAATTTTTGGTCTTCTCATTTACCAAGAACTGTGTATGATACTGAATTAGATGCTGATAGCCCTAATCCAAATGATCAGGGTTTTGAAAAATTGATATCGGGAATGTACCTGGGCGATGTTGTTAGGAGAGTGATTGTCAGAATGTCACAGGAGTCGGAAATTTTTGAGCCTATATCTTCCAAGTTATATATGCCTTTCATTTTGAGGACGCCGTTGATTGCTGCAATGCATGAGGATGACTCTCCAGACTTGGAAGAAGTAGGACGCATTTTAAGAGAAAACCTGGAGATTCCGGATGTCCCTCTGAAGGTCCGAAAGCTCATAGTCAAGGTGTGTGACGTTGTGACGCGCAGGGCTGCTCGATTGGCAGCTGCTGGTATTGTGGGGATCTTAAAGAAGATTGGCCGGGATGGGAGTGGTGGAATCACAGGCGGAAGAAGTAGAAGCGATCACAGAATGAGAAGAACGGTTGTGGCAATGGAGGGGGGTTTGTATACAGAATATACAATGTTTAGAGAGTACTTGCATGAAGCGTTGACGGAAATATTAGGCGAAGATATTGCGAAACATGTCGTACTCATCAAGGTCACGGAGGACGGATCGGCCACTGGAGCAGCTCTGCTAGCTGCTTCACATTCATCCTACCCAGTGGATAACGTACGGTTGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001678 GO:0003674 GO:0003824 GO:0004340 GO:0004396 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008865 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009409 GO:0009536 GO:0009628 GO:0009651 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016020 GO:0016021 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019158 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019725 GO:0019752 GO:0019867 GO:0021700 GO:0022622 GO:0030154 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032501 GO:0032502 GO:0032592 GO:0032787 GO:0033500 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042592 GO:0042593 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0048364 GO:0048468 GO:0048469 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0048878 GO:0050896 GO:0051156 GO:0051186 GO:0051188 GO:0055082 GO:0055086 GO:0065007 GO:0065008 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080147 GO:0090407 GO:0090558 GO:0090627 GO:0098573 GO:0098588 GO:0098805 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00052 ko00500 ko00520 ko00521 ko00524 ko01100 ko01110 ko01120 ko01130 ko01200 ko04066 ko04910 ko04930 ko04973 ko05230 map00010 map00051 map00052 map00500 map00520 map00521 map00524 map01100 map01110 map01120 map01130 map01200 map04066 map04910 map04930 map04973 map05230
Pfam Domains
Protein Families
Hexokinase_1 Hexokinase_2

Protein Analysis

510

Amino Acids

55.5

Weight (kDa)

6.62

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hexokinase_1 PF00349 41 - 240 3.3e-69 Hexokinase
Hexokinase_2 PF03727 247 - 496 1.7e-76 Hexokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018755)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50460 AT1G50460 AT1G50460
malus_domestica MD03G1170700.v1.1 MD11G1188100.v1.1
prunus_persica Prupe.4G256200_v2.0.a1 Prupe.4G256200_v2.0.a1
pyrus_communis pycom03g12760 pycom11g16500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 160
Acc36I ACCTGC 1 cut(s) 160
AccI GTMKAC 1 cut(s) 1352
AccII CGCG 1 cut(s) 1207
AccIII TCCGGA 1 cut(s) 1150
AciI CCGC 9 cut(s) 14, 26, 38, 44, 53, 188, 309, 331, 1290
AclWI GGATC 2 cut(s) 1253, 1465
AcoI YGGCCR 2 cut(s) 1261, 1461
AcsI RAATTY 3 cut(s) 400, 859, 1017
AcuI CTGAAG 1 cut(s) 1184
AcyI GRCGYC 2 cut(s) 180, 1067
AfaI GTAC 7 cut(s) 313, 349, 378, 965, 1379, 1434, 1518
AfiI CCNNNNNNNGG 2 cut(s) 156, 1165
AflIII ACRYGT 1 cut(s) 1426
AgsI TTSAA 4 cut(s) 124, 467, 599, 944
AjnI CCWGG 3 cut(s) 129, 966, 1140
AjuI GAANNNNNNNTTGG 2 cut(s) 591, 623
AleI CACNNNNGTG 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 810
AlwI GGATC 2 cut(s) 1253, 1465
AlwNI CAGNNNCTG 3 cut(s) 734, 827, 1232
Aor13HI TCCGGA 1 cut(s) 1150
AoxI GGCC 3 cut(s) 210, 1261, 1461
ApeKI GCWGC 9 cut(s) 241, 674, 740, 1079, 1214, 1226, 1229, 1472, 1484
ApoI RAATTY 3 cut(s) 400, 859, 1017
AspLEI GCGC 1 cut(s) 1209
AspS9I GGNCC 3 cut(s) 127, 354, 1168
AsuC2I CCSGG 1 cut(s) 1265
AsuNHI GCTAGC 1 cut(s) 1480
AvaII GGWCC 3 cut(s) 127, 354, 1168
BbsI GAAGAC 2 cut(s) 606, 861
BbvI GCAGC 9 cut(s) 228, 686, 727, 1066, 1201, 1216, 1238, 1471, 1484
BccI CCATC 3 cut(s) 58, 263, 1262
BceAI ACGGC 1 cut(s) 1054
BcgI CGANNNNNNTGC 2 cut(s) 1400, 1434
BciT130I CCWGG 3 cut(s) 131, 968, 1142
BclI TGATCA 1 cut(s) 931
BcnI CCSGG 1 cut(s) 1265
BcoDI GTCTC 1 cut(s) 810
BfaI CTAG 2 cut(s) 299, 1481
BfmI CTRYAG 1 cut(s) 741
BfuAI ACCTGC 1 cut(s) 160
BglII AGATCT 1 cut(s) 301
BlpI GCTNAGC 1 cut(s) 137
BmcAI AGTACT 1 cut(s) 1379
Bme1390I CCNGG 4 cut(s) 131, 968, 1142, 1265
Bme18I GGWCC 3 cut(s) 127, 354, 1168
BmgT120I GGNCC 3 cut(s) 127, 354, 1168
BmiI GGNNCC 1 cut(s) 226
BmrFI CCNGG 4 cut(s) 131, 968, 1142, 1265
BmrI ACTGGG 2 cut(s) 420, 1499
BmsI GCATC 5 cut(s) 186, 617, 781, 833, 898
BmtI GCTAGC 1 cut(s) 1484
BmuI ACTGGG 2 cut(s) 420, 1499
BpiI GAAGAC 2 cut(s) 606, 861
BplI GAGNNNNNCTC 2 cut(s) 1082, 1114
BpmI CTGGAG 5 cut(s) 152, 849, 1086, 1163, 1488
Bpu1102I GCTNAGC 1 cut(s) 137
BpuEI CTTGAG 1 cut(s) 792
BpuMI CCSGG 1 cut(s) 1265
BsaBI GATNNNNATC 1 cut(s) 378
BsaHI GRCGYC 2 cut(s) 180, 1067
BsaJI CCNNGG 3 cut(s) 183, 363, 967
BsaWI WCCGGW 1 cut(s) 1150
BsaXI ACNNNNNCTCC 4 cut(s) 209, 239, 822, 852
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 1165
Bse1I ACTGG 5 cut(s) 415, 536, 763, 1471, 1505
Bse3DI GCAATG 3 cut(s) 648, 1089, 1341
Bse8I GATNNNNATC 1 cut(s) 378
BseAI TCCGGA 1 cut(s) 1150
BseBI CCWGG 3 cut(s) 131, 968, 1142
BseDI CCNNGG 3 cut(s) 183, 363, 967
BseGI GGATG 5 cut(s) 632, 1099, 1159, 1273, 1496
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 2 cut(s) 156, 1165
BseMI GCAATG 3 cut(s) 648, 1089, 1341
BseNI ACTGG 5 cut(s) 415, 536, 763, 1471, 1505
BseXI GCAGC 9 cut(s) 228, 686, 727, 1066, 1201, 1216, 1238, 1471, 1484
Bsh1236I CGCG 1 cut(s) 1207
BshFI GGCC 3 cut(s) 212, 1263, 1463
BsiSI CCGG 2 cut(s) 1151, 1264
BsiWI CGTACG 1 cut(s) 1516
BslFI GGGAC 2 cut(s) 427, 1142
BslI CCNNNNNNNGG 2 cut(s) 156, 1165
BsmAI GTCTC 1 cut(s) 810
BsmFI GGGAC 2 cut(s) 427, 1142
BsnI GGCC 3 cut(s) 212, 1263, 1463
Bsp13I TCCGGA 1 cut(s) 1150
Bsp1407I TGTACA 1 cut(s) 376
Bsp143I GATC 5 cut(s) 301, 931, 1245, 1306, 1457
Bsp1720I GCTNAGC 1 cut(s) 137
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 9 cut(s) 14, 26, 38, 44, 53, 188, 309, 331, 1290
BspANI GGCC 3 cut(s) 212, 1263, 1463
BspEI TCCGGA 1 cut(s) 1150
BspFNI CGCG 1 cut(s) 1207
BspLI GGNNCC 1 cut(s) 226
BspMAI CTGCAG 1 cut(s) 745
BspMI ACCTGC 1 cut(s) 160
BspOI GCTAGC 1 cut(s) 1484
BspPI GGATC 2 cut(s) 1253, 1465
BsrDI GCAATG 3 cut(s) 648, 1089, 1341
BsrGI TGTACA 1 cut(s) 376
BsrI ACTGG 5 cut(s) 415, 536, 763, 1471, 1505
BssECI CCNNGG 3 cut(s) 183, 363, 967
BssMI GATC 5 cut(s) 301, 931, 1245, 1306, 1457
BssNAI GTATAC 1 cut(s) 1353
BssNI GRCGYC 2 cut(s) 180, 1067
BssT1I CCWWGG 2 cut(s) 183, 363
Bst1107I GTATAC 1 cut(s) 1353
Bst2UI CCWGG 3 cut(s) 131, 968, 1142
Bst4CI ACNGT 6 cut(s) 154, 221, 736, 889, 1327, 1521
Bst6I CTCTTC 1 cut(s) 100
BstACI GRCGYC 2 cut(s) 180, 1067
BstAUI TGTACA 1 cut(s) 376
BstC8I GCNNGC 3 cut(s) 201, 205, 1482
BstDEI CTNAG 2 cut(s) 137, 486
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 5 cut(s) 632, 1099, 1159, 1273, 1496
BstFNI CGCG 1 cut(s) 1207
BstHHI GCGC 1 cut(s) 1209
BstKTI GATC 5 cut(s) 304, 934, 1248, 1309, 1460
BstMAI GTCTC 1 cut(s) 810
BstMBI GATC 5 cut(s) 301, 931, 1245, 1306, 1457
BstMWI GCNNNNNNNGC 8 cut(s) 44, 50, 209, 238, 337, 1223, 1469, 1481
BstNI CCWGG 3 cut(s) 131, 968, 1142
BstNSI RCATGY 1 cut(s) 1430
BstSCI CCNGG 4 cut(s) 129, 966, 1140, 1263
BstSFI CTRYAG 1 cut(s) 741
BstUI CGCG 1 cut(s) 1207
BstV1I GCAGC 9 cut(s) 228, 686, 727, 1066, 1201, 1216, 1238, 1471, 1484
BstV2I GAAGAC 2 cut(s) 606, 861
BstX2I RGATCY 2 cut(s) 301, 1245
BstXI CCANNNNNNTGG 1 cut(s) 190
BstYI RGATCY 2 cut(s) 301, 1245
BstZ17I GTATAC 1 cut(s) 1353
BsuRI GGCC 3 cut(s) 212, 1263, 1463
BtgI CCRYGG 1 cut(s) 183
BtgZI GCGATG 1 cut(s) 987
BtsCI GGATG 5 cut(s) 632, 1099, 1159, 1273, 1496
BtsIMutI CAGTG 5 cut(s) 159, 543, 732, 1464, 1512
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 3 cut(s) 201, 205, 1482
CaiI CAGNNNCTG 3 cut(s) 734, 827, 1232
CfoI GCGC 1 cut(s) 1209
Cfr13I GGNCC 3 cut(s) 127, 354, 1168
CseI GACGC 4 cut(s) 188, 1075, 1131, 1213
Csp6I GTAC 7 cut(s) 312, 348, 377, 964, 1378, 1433, 1517
CviAII CATG 8 cut(s) 184, 371, 604, 835, 1088, 1385, 1427, 1527
CviQI GTAC 7 cut(s) 312, 348, 377, 964, 1378, 1433, 1517
DdeI CTNAG 2 cut(s) 137, 486
DpnI GATC 5 cut(s) 303, 933, 1247, 1308, 1459
DpnII GATC 5 cut(s) 301, 931, 1245, 1306, 1457
EaeI YGGCCR 2 cut(s) 1261, 1461
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
EciI GGCGGA 1 cut(s) 1305
Eco130I CCWWGG 2 cut(s) 183, 363
Eco32I GATATC 1 cut(s) 954
Eco47I GGWCC 3 cut(s) 127, 354, 1168
Eco57I CTGAAG 1 cut(s) 1184
EcoO109I RGGNCCY 2 cut(s) 127, 354
EcoRII CCWGG 3 cut(s) 129, 966, 1140
EcoRV GATATC 1 cut(s) 954
EcoT14I CCWWGG 2 cut(s) 183, 363
EcoT22I ATGCAT 1 cut(s) 1089
ErhI CCWWGG 2 cut(s) 183, 363
FaeI CATG 8 cut(s) 187, 374, 607, 838, 1091, 1388, 1430, 1530
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 2 cut(s) 427, 1142
FatI CATG 8 cut(s) 183, 370, 603, 834, 1087, 1384, 1426, 1526
FauI CCCGC 1 cut(s) 324
FbaI TGATCA 1 cut(s) 931
FblI GTMKAC 1 cut(s) 1352
FokI GGATG 5 cut(s) 639, 1106, 1166, 1280, 1483
FspBI CTAG 2 cut(s) 299, 1481
GlaI GCGC 1 cut(s) 1208
GsuI CTGGAG 5 cut(s) 152, 849, 1086, 1163, 1488
HaeIII GGCC 3 cut(s) 212, 1263, 1463
HapII CCGG 2 cut(s) 1151, 1264
HgaI GACGC 4 cut(s) 188, 1075, 1131, 1213
HhaI GCGC 1 cut(s) 1209
Hin1I GRCGYC 2 cut(s) 180, 1067
Hin1II CATG 8 cut(s) 187, 374, 607, 838, 1091, 1388, 1430, 1530
Hin6I GCGC 1 cut(s) 1207
HinP1I GCGC 1 cut(s) 1207
HincII GTYRAC 1 cut(s) 1395
HindII GTYRAC 1 cut(s) 1395
HindIII AAGCTT 2 cut(s) 338, 488
HinfI GANTC 6 cut(s) 482, 689, 1010, 1097, 1147, 1281
HpaII CCGG 2 cut(s) 1151, 1264
Hpy166II GTNNAC 3 cut(s) 178, 1353, 1395
Hpy188I TCNGA 6 cut(s) 78, 217, 998, 1015, 1164, 1172
Hpy188III TCNNGA 6 cut(s) 121, 722, 828, 957, 1103, 1151
Hpy8I GTNNAC 3 cut(s) 178, 1353, 1395
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 5 cut(s) 87, 118, 501, 646, 1159
HpyCH4III ACNGT 6 cut(s) 154, 221, 736, 889, 1327, 1521
HpyCH4IV ACGT 2 cut(s) 1197, 1515
HpyCH4V TGCA 7 cut(s) 199, 296, 623, 743, 1082, 1087, 1384
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 50, 209, 238, 337, 1223, 1469, 1481
HpyF3I CTNAG 2 cut(s) 137, 486
HpySE526I ACGT 2 cut(s) 1197, 1515
Hsp92I GRCGYC 2 cut(s) 180, 1067
Hsp92II CATG 8 cut(s) 187, 374, 607, 838, 1091, 1388, 1430, 1530
HspAI GCGC 1 cut(s) 1207
Kpn2I TCCGGA 1 cut(s) 1150
Ksp22I TGATCA 1 cut(s) 931
Kzo9I GATC 5 cut(s) 301, 931, 1245, 1306, 1457
LmnI GCTCC 6 cut(s) 87, 133, 230, 273, 511, 1469
Lsp1109I GCAGC 9 cut(s) 228, 686, 727, 1066, 1201, 1216, 1238, 1471, 1484
LweI GCATC 5 cut(s) 186, 617, 781, 833, 898
MaeI CTAG 2 cut(s) 299, 1481
MaeII ACGT 2 cut(s) 1197, 1515
MaeIII GTNAC 4 cut(s) 1002, 1193, 1201, 1444
MalI GATC 5 cut(s) 303, 933, 1247, 1308, 1459
MboI GATC 5 cut(s) 301, 931, 1245, 1306, 1457
MflI RGATCY 2 cut(s) 301, 1245
MluCI AATT 7 cut(s) 400, 750, 798, 859, 902, 947, 1017
MlyI GAGTC 3 cut(s) 683, 1019, 1091
MmeI TCCRAC 2 cut(s) 56, 993
Mph1103I ATGCAT 1 cut(s) 1089
MroI TCCGGA 1 cut(s) 1150
MseI TTAA 5 cut(s) 336, 572, 801, 1130, 1250
MslI CAYNNNNRTG 3 cut(s) 188, 251, 1092
MspA1I CMGCKG 1 cut(s) 1229
MspI CCGG 2 cut(s) 1151, 1264
MspR9I CCNGG 4 cut(s) 131, 968, 1142, 1265
MvaI CCWGG 3 cut(s) 131, 968, 1142
MvnI CGCG 1 cut(s) 1207
MwoI GCNNNNNNNGC 8 cut(s) 44, 50, 209, 238, 337, 1223, 1469, 1481
NciI CCSGG 1 cut(s) 1265
NcoI CCATGG 1 cut(s) 183
NdeII GATC 5 cut(s) 301, 931, 1245, 1306, 1457
NheI GCTAGC 1 cut(s) 1480
NlaIII CATG 8 cut(s) 187, 374, 607, 838, 1091, 1388, 1430, 1530
NlaIV GGNNCC 1 cut(s) 226
NmuCI GTSAC 4 cut(s) 1002, 1193, 1201, 1444
NsiI ATGCAT 1 cut(s) 1089
NspI RCATGY 1 cut(s) 1430
OliI CACNNNNGTG 1 cut(s) 251
PaqCI CACCTGC 1 cut(s) 160
PciI ACATGT 1 cut(s) 1426
PfeI GAWTC 3 cut(s) 482, 1147, 1281
Pfl23II CGTACG 1 cut(s) 1516
PleI GAGTC 3 cut(s) 683, 1018, 1091
PpsI GAGTC 3 cut(s) 683, 1018, 1091
PpuMI RGGWCCY 2 cut(s) 127, 354
PscI ACATGT 1 cut(s) 1426
Psp5II RGGWCCY 2 cut(s) 127, 354
Psp6I CCWGG 3 cut(s) 129, 966, 1140
PspGI CCWGG 3 cut(s) 129, 966, 1140
PspLI CGTACG 1 cut(s) 1516
PspN4I GGNNCC 1 cut(s) 226
PspPI GGNCC 3 cut(s) 127, 354, 1168
PspPPI RGGWCCY 2 cut(s) 127, 354
PsrI GAACNNNNNNTAC 2 cut(s) 360, 392
PstI CTGCAG 1 cut(s) 745
PstNI CAGNNNCTG 3 cut(s) 734, 827, 1232
PsuI RGATCY 2 cut(s) 301, 1245
PvuII CAGCTG 1 cut(s) 1229
RsaI GTAC 7 cut(s) 313, 349, 378, 965, 1379, 1434, 1518
RsaNI GTAC 7 cut(s) 312, 348, 377, 964, 1378, 1433, 1517
RseI CAYNNNNRTG 3 cut(s) 188, 251, 1092
SaqAI TTAA 5 cut(s) 336, 572, 801, 1130, 1250
Sau3AI GATC 5 cut(s) 301, 931, 1245, 1306, 1457
Sau96I GGNCC 3 cut(s) 127, 354, 1168
ScaI AGTACT 1 cut(s) 1379
SchI GAGTC 3 cut(s) 683, 1019, 1091
ScrFI CCNGG 4 cut(s) 131, 968, 1142, 1265
SfaNI GCATC 5 cut(s) 186, 617, 781, 833, 898
SfcI CTRYAG 1 cut(s) 741
SinI GGWCC 3 cut(s) 127, 354, 1168
SmiMI CAYNNNNRTG 3 cut(s) 188, 251, 1092
SmlI CTYRAG 1 cut(s) 807
SmoI CTYRAG 1 cut(s) 807
Sse9I AATT 7 cut(s) 400, 750, 798, 859, 902, 947, 1017
SsiI CCGC 9 cut(s) 14, 26, 38, 44, 53, 188, 309, 331, 1290
SspI AATATT 1 cut(s) 1404
SspMI CTAG 2 cut(s) 299, 1481
StyD4I CCNGG 4 cut(s) 129, 966, 1140, 1263
StyI CCWWGG 2 cut(s) 183, 363
TaaI ACNGT 6 cut(s) 154, 221, 736, 889, 1327, 1521
TaiI ACGT 2 cut(s) 1200, 1518
TaqI TCGA 1 cut(s) 1219
TasI AATT 7 cut(s) 400, 750, 798, 859, 902, 947, 1017
TatI WGTACW 2 cut(s) 376, 1377
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 3 cut(s) 482, 1147, 1281
Tru1I TTAA 5 cut(s) 336, 572, 801, 1130, 1250
Tru9I TTAA 5 cut(s) 336, 572, 801, 1130, 1250
TscAI CASTG 5 cut(s) 159, 543, 739, 1471, 1512
TseFI GTSAC 4 cut(s) 1002, 1193, 1201, 1444
TseI GCWGC 9 cut(s) 241, 674, 740, 1079, 1214, 1226, 1229, 1472, 1484
Tsp45I GTSAC 4 cut(s) 1002, 1193, 1201, 1444
TspDTI ATGAA 5 cut(s) 435, 546, 1045, 1401, 1485
TspGWI ACGGA 3 cut(s) 1412, 1463, 1470
TspRI CASTG 5 cut(s) 159, 543, 739, 1471, 1512
VpaK11BI GGWCC 3 cut(s) 127, 354, 1168
XapI RAATTY 3 cut(s) 400, 859, 1017
XceI RCATGY 1 cut(s) 1430
XmiI GTMKAC 1 cut(s) 1352
XspI CTAG 2 cut(s) 299, 1481
ZrmI AGTACT 1 cut(s) 1379
Zsp2I ATGCAT 1 cut(s) 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.