MD03G1194900.v1.1

histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0019551
Physical Location & Seq
Reverse (-)
26690008 .. 26690418
411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1194900.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCCCGTACAAAGCAAACTGCTCGCAAGTCCACCGGAGGCAAGGCTCCAAGGAAGCAACTTGCGGCCACGATATATAGGAAAGCCGCCCCAACCACCGGAGGCGTGAAGAAGCCGCACAGATACCGCCCTGGAACCGTCGCTCTCCGTGAAATCCGCAAGTACCAGAAGAGCACAGAGCTTCTGATCAGGAAGCTGCCGTTCCAGAGGCTGGTTCGTGAGATAGCGCAGGACTTCATGACGGATCTGCGTTTCCAGAGCCACGCTGTGTTTGCACTGCAGGAGGCTGCGGAGGCGTACCTTGTTGGGTTGTTTGAGGACACTAACCTGTGCGCCATTCATGCCAGGCGCGTCACGATCATGCCCAAGGATGTCCAACTTGCCAGGAGAATCCGGGGCGAGAGGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

137

Amino Acids

15.52

Weight (kDa)

11.12

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 44 - 131 2.8e-36 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13370 AT1G75600
malus_domestica MD03G1194900.v1.1 MD09G1242000.v1.1
pyrus_communis pycom03g14700 pycom09g16060
rosa_laevigata RLG00000011176

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 211
AccII CGCG 1 cut(s) 351
AciI CCGC 6 cut(s) 65, 87, 116, 127, 157, 290
AclWI GGATC 1 cut(s) 252
AcoI YGGCCR 1 cut(s) 66
AdeI CACNNNGTG 1 cut(s) 268
AfaI GTAC 3 cut(s) 10, 164, 299
AfiI CCNNNNNNNGG 2 cut(s) 98, 211
AjnI CCWGG 3 cut(s) 130, 344, 383
AluBI AGCT 2 cut(s) 181, 196
AluI AGCT 2 cut(s) 181, 196
Alw21I GWGCWC 1 cut(s) 176
AlwI GGATC 1 cut(s) 252
AlwNI CAGNNNCTG 1 cut(s) 211
AoxI GGCC 2 cut(s) 3, 66
ApeKI GCWGC 2 cut(s) 196, 287
AspLEI GCGC 3 cut(s) 229, 335, 351
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 2 cut(s) 183, 274
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 3 cut(s) 132, 346, 385
BclI TGATCA 1 cut(s) 186
BcnI CCSGG 1 cut(s) 395
BfmI CTRYAG 1 cut(s) 278
BisI GCNGC 5 cut(s) 66, 87, 116, 197, 288
BlsI GCNGC 5 cut(s) 67, 88, 117, 198, 289
Bme1390I CCNGG 4 cut(s) 132, 346, 385, 395
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 48, 136
BmrFI CCNGG 4 cut(s) 132, 346, 385, 395
BpuMI CCSGG 1 cut(s) 395
BsaJI CCNNGG 4 cut(s) 50, 130, 366, 394
BsaWI WCCGGW 2 cut(s) 35, 98
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 211
BseBI CCWGG 3 cut(s) 132, 346, 385
BseDI CCNNGG 4 cut(s) 50, 130, 366, 394
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 2 cut(s) 98, 211
BseXI GCAGC 2 cut(s) 183, 274
Bsh1236I CGCG 1 cut(s) 351
BshFI GGCC 2 cut(s) 5, 68
BsiHKAI GWGCWC 1 cut(s) 176
BsiSI CCGG 3 cut(s) 36, 99, 394
BslI CCNNNNNNNGG 2 cut(s) 98, 211
BsnI GGCC 2 cut(s) 5, 68
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 3 cut(s) 186, 244, 357
BspACI CCGC 6 cut(s) 65, 87, 116, 127, 157, 290
BspANI GGCC 2 cut(s) 5, 68
BspFNI CGCG 1 cut(s) 351
BspHI TCATGA 1 cut(s) 237
BspLI GGNNCC 2 cut(s) 48, 136
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 1 cut(s) 252
BspQI GCTCTTC 1 cut(s) 164
BssECI CCNNGG 4 cut(s) 50, 130, 366, 394
BssMI GATC 3 cut(s) 186, 244, 357
BssT1I CCWWGG 2 cut(s) 50, 366
Bst2UI CCWGG 3 cut(s) 132, 346, 385
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 25
BstF5I GGATG 1 cut(s) 376
BstFNI CGCG 1 cut(s) 351
BstHHI GCGC 3 cut(s) 229, 335, 351
BstKTI GATC 3 cut(s) 189, 247, 360
BstMBI GATC 3 cut(s) 186, 244, 357
BstMWI GCNNNNNNNGC 3 cut(s) 272, 293, 341
BstNI CCWGG 3 cut(s) 132, 346, 385
BstSCI CCNGG 4 cut(s) 130, 344, 383, 393
BstSFI CTRYAG 1 cut(s) 278
BstUI CGCG 1 cut(s) 351
BstV1I GCAGC 2 cut(s) 183, 274
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
BsuRI GGCC 2 cut(s) 5, 68
BtsCI GGATG 1 cut(s) 376
BtsI GCAGTG 1 cut(s) 275
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 1 cut(s) 25
CaiI CAGNNNCTG 1 cut(s) 211
CciI TCATGA 1 cut(s) 237
CfoI GCGC 3 cut(s) 229, 335, 351
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 340
Csp6I GTAC 3 cut(s) 9, 163, 298
CviAII CATG 3 cut(s) 238, 341, 361
CviQI GTAC 3 cut(s) 9, 163, 298
DpnI GATC 3 cut(s) 188, 246, 359
DpnII GATC 3 cut(s) 186, 244, 357
DraIII CACNNNGTG 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
Eco130I CCWWGG 2 cut(s) 50, 366
EcoRII CCWGG 3 cut(s) 130, 344, 383
EcoT14I CCWWGG 2 cut(s) 50, 366
ErhI CCWWGG 2 cut(s) 50, 366
FaeI CATG 3 cut(s) 241, 344, 364
FaiI YATR 5 cut(s) 76, 78, 239, 342, 362
FatI CATG 3 cut(s) 237, 340, 360
FbaI TGATCA 1 cut(s) 186
Fnu4HI GCNGC 5 cut(s) 66, 87, 116, 197, 288
FokI GGATG 1 cut(s) 383
Fsp4HI GCNGC 5 cut(s) 66, 87, 116, 197, 288
GlaI GCGC 3 cut(s) 228, 334, 350
GluI GCNGC 5 cut(s) 66, 87, 116, 197, 288
HaeIII GGCC 2 cut(s) 5, 68
HapII CCGG 3 cut(s) 36, 99, 394
HgaI GACGC 1 cut(s) 340
HhaI GCGC 3 cut(s) 229, 335, 351
Hin1II CATG 3 cut(s) 241, 344, 364
Hin6I GCGC 3 cut(s) 227, 333, 349
HinP1I GCGC 3 cut(s) 227, 333, 349
HinfI GANTC 1 cut(s) 390
HpaII CCGG 3 cut(s) 36, 99, 394
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 6 cut(s) 190, 205, 218, 238, 256, 355
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 1 cut(s) 143
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 2 cut(s) 275, 280
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 293, 341
Hsp92II CATG 3 cut(s) 241, 344, 364
HspAI GCGC 3 cut(s) 227, 333, 349
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 3 cut(s) 186, 244, 357
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 1 cut(s) 52
Lsp1109I GCAGC 2 cut(s) 183, 274
MaeIII GTNAC 1 cut(s) 352
MalI GATC 3 cut(s) 188, 246, 359
MboI GATC 3 cut(s) 186, 244, 357
MboII GAAGA 2 cut(s) 121, 181
MflI RGATCY 1 cut(s) 244
MhlI GDGCHC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 7 cut(s) 32, 95, 201, 277, 286, 310, 396
MseI TTAA 1 cut(s) 409
MslI CAYNNNNRTG 1 cut(s) 359
MspI CCGG 3 cut(s) 36, 99, 394
MspR9I CCNGG 4 cut(s) 132, 346, 385, 395
MvaI CCWGG 3 cut(s) 132, 346, 385
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 3 cut(s) 272, 293, 341
NciI CCSGG 1 cut(s) 395
NdeII GATC 3 cut(s) 186, 244, 357
NlaIII CATG 3 cut(s) 241, 344, 364
NlaIV GGNNCC 2 cut(s) 48, 136
NmuCI GTSAC 1 cut(s) 352
PagI TCATGA 1 cut(s) 237
PciSI GCTCTTC 1 cut(s) 164
PfeI GAWTC 1 cut(s) 390
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 5 cut(s) 67, 88, 117, 198, 289
Psp6I CCWGG 3 cut(s) 130, 344, 383
PspGI CCWGG 3 cut(s) 130, 344, 383
PspN4I GGNNCC 2 cut(s) 48, 136
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 282
PstNI CAGNNNCTG 1 cut(s) 211
PsuI RGATCY 1 cut(s) 244
RsaI GTAC 3 cut(s) 10, 164, 299
RsaNI GTAC 3 cut(s) 9, 163, 298
RseI CAYNNNNRTG 1 cut(s) 359
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 409
SatI GCNGC 5 cut(s) 66, 87, 116, 197, 288
Sau3AI GATC 3 cut(s) 186, 244, 357
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 4 cut(s) 132, 346, 385, 395
SduI GDGCHC 1 cut(s) 176
SetI ASST 4 cut(s) 183, 198, 303, 330
SfcI CTRYAG 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 359
SsiI CCGC 6 cut(s) 65, 87, 116, 127, 157, 290
StyD4I CCNGG 4 cut(s) 130, 344, 383, 393
StyI CCWWGG 2 cut(s) 50, 366
TaaI ACNGT 1 cut(s) 139
TauI GCSGC 3 cut(s) 68, 89, 118
TfiI GAWTC 1 cut(s) 390
Tru1I TTAA 1 cut(s) 409
Tru9I TTAA 1 cut(s) 409
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 196, 287
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 2 cut(s) 226, 329
TspGWI ACGGA 2 cut(s) 137, 257
TspRI CASTG 1 cut(s) 282
Van91I CCANNNNNTGG 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.