MD09G1242000.v1.1

Histone H3.3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0019551
Physical Location & Seq
Reverse (-)
30937067 .. 30937766
700 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1242000.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCTCGTACGAAGCAAACTGCTCGTAAATCCACCGGCGGTAAGGCTCCGAGGAAGTCACTTGCCTTCAAGGCTGCAAGGAAATCAACACCCACAACCGGAGGAGTCAAGAAGCCTCACCGCTACCGCCCTGGAACCGTCGCTCTTCGCGAAATTCGAAAATACCAGAAGAGCACTGAGCTTCTGATCCGCAAGCTGCCGTTCCAACGCCTTGTTCGTGAACTCGCACAGGACTACAAGACGGACCTGCGGTTCCAGAGCCATGCTATTCTTGCTCTCCAGGAAGCTGCGGAGGCTTATTTGGTTGGACTGTTTGAGGACACCAACCTTTGCGCCATTCATGCCAAAAGAGTTACCATAATGCCAAAGGACGTTCAGCTCGCTAGGAGGATCCGTGGTGAACGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

137

Amino Acids

15.46

Weight (kDa)

11.02

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 44 - 131 1.8e-37 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13370 AT1G75600
malus_domestica MD03G1194900.v1.1 MD09G1242000.v1.1
pyrus_communis pycom03g14700 pycom09g16060
rosa_laevigata RLG00000011176

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 255
AccII CGCG 1 cut(s) 150
AciI CCGC 6 cut(s) 39, 121, 127, 190, 250, 290
AclWI GGATC 3 cut(s) 181, 385, 398
AcsI RAATTY 1 cut(s) 153
AfaI GTAC 1 cut(s) 10
AfiI CCNNNNNNNGG 1 cut(s) 98
AgsI TTSAA 1 cut(s) 70
AjnI CCWGG 2 cut(s) 130, 279
AjuI GAANNNNNNNTTGG 2 cut(s) 198, 230
AloI GAACNNNNNNTCC 2 cut(s) 236, 268
AluBI AGCT 4 cut(s) 181, 196, 287, 379
AluI AGCT 4 cut(s) 181, 196, 287, 379
Alw21I GWGCWC 1 cut(s) 176
AlwI GGATC 3 cut(s) 181, 385, 398
ApeKI GCWGC 3 cut(s) 74, 196, 287
ApoI RAATTY 1 cut(s) 153
AspLEI GCGC 1 cut(s) 335
AspS9I GGNCC 1 cut(s) 244
AsuHPI GGTGA 2 cut(s) 110, 410
AsuII TTCGAA 1 cut(s) 157
AvaII GGWCC 1 cut(s) 244
BamHI GGATCC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 3 cut(s) 61, 183, 274
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 2 cut(s) 132, 281
BfaI CTAG 1 cut(s) 384
BfuAI ACCTGC 1 cut(s) 255
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 3 cut(s) 75, 197, 288
BlsI GCNGC 3 cut(s) 76, 198, 289
Bme1390I CCNGG 2 cut(s) 132, 281
Bme18I GGWCC 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 244
BmiI GGNNCC 4 cut(s) 48, 136, 254, 392
BmrFI CCNGG 2 cut(s) 132, 281
BpmI CTGGAG 1 cut(s) 263
Bpu14I TTCGAA 1 cut(s) 157
BsaJI CCNNGG 3 cut(s) 50, 130, 394
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 1 cut(s) 98
Bse118I RCCGGY 1 cut(s) 35
BseBI CCWGG 2 cut(s) 132, 281
BseDI CCNNGG 3 cut(s) 50, 130, 394
BseLI CCNNNNNNNGG 1 cut(s) 98
BseMII CTCAG 1 cut(s) 168
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 3 cut(s) 61, 183, 274
Bsh1236I CGCG 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 176
BsiSI CCGG 2 cut(s) 36, 99
BsiWI CGTACG 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 157
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 2 cut(s) 186, 390
Bsp68I TCGCGA 1 cut(s) 150
BspACI CCGC 6 cut(s) 39, 121, 127, 190, 250, 290
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 4 cut(s) 48, 136, 254, 392
BspMI ACCTGC 1 cut(s) 255
BspPI GGATC 3 cut(s) 181, 385, 398
BspQI GCTCTTC 2 cut(s) 150, 164
BspT104I TTCGAA 1 cut(s) 157
BsrFI RCCGGY 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 3 cut(s) 50, 130, 394
BssMI GATC 2 cut(s) 186, 390
Bst2UI CCWGG 2 cut(s) 132, 281
Bst4CI ACNGT 2 cut(s) 139, 312
Bst6I CTCTTC 2 cut(s) 150, 164
BstBI TTCGAA 1 cut(s) 157
BstC8I GCNNGC 2 cut(s) 194, 381
BstDEI CTNAG 1 cut(s) 177
BstDSI CCRYGG 1 cut(s) 394
BstFNI CGCG 1 cut(s) 150
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 2 cut(s) 189, 393
BstMBI GATC 2 cut(s) 186, 390
BstMWI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
BstNI CCWGG 2 cut(s) 132, 281
BstSCI CCNGG 2 cut(s) 130, 279
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 3 cut(s) 61, 183, 274
BstX2I RGATCY 1 cut(s) 390
BstYI RGATCY 1 cut(s) 390
BtgI CCRYGG 1 cut(s) 394
BtsIMutI CAGTG 1 cut(s) 174
BtuMI TCGCGA 1 cut(s) 150
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 194, 381
CfoI GCGC 1 cut(s) 335
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 244
Csp6I GTAC 1 cut(s) 9
CviAII CATG 2 cut(s) 263, 341
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 1 cut(s) 177
DpnI GATC 2 cut(s) 188, 392
DpnII GATC 2 cut(s) 186, 390
Eam1104I CTCTTC 2 cut(s) 150, 164
EarI CTCTTC 2 cut(s) 150, 164
Eco47I GGWCC 1 cut(s) 244
EcoRII CCWGG 2 cut(s) 130, 279
FaeI CATG 2 cut(s) 266, 344
FaiI YATR 3 cut(s) 264, 342, 359
FatI CATG 2 cut(s) 262, 340
Fnu4HI GCNGC 3 cut(s) 75, 197, 288
Fsp4HI GCNGC 3 cut(s) 75, 197, 288
FspBI CTAG 1 cut(s) 384
GlaI GCGC 1 cut(s) 334
GluI GCNGC 3 cut(s) 75, 197, 288
GsuI CTGGAG 1 cut(s) 263
HapII CCGG 2 cut(s) 36, 99
HhaI GCGC 1 cut(s) 335
Hin1II CATG 2 cut(s) 266, 344
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HinfI GANTC 1 cut(s) 105
HpaII CCGG 2 cut(s) 36, 99
HphI GGTGA 2 cut(s) 110, 410
Hpy166II GTNNAC 2 cut(s) 221, 401
Hpy188I TCNGA 2 cut(s) 51, 186
Hpy188III TCNNGA 4 cut(s) 109, 149, 218, 256
Hpy8I GTNNAC 2 cut(s) 221, 401
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 1 cut(s) 76
HpyCH4III ACNGT 2 cut(s) 139, 312
HpyCH4IV ACGT 2 cut(s) 372, 403
HpyCH4V TGCA 1 cut(s) 77
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 2 cut(s) 372, 403
Hsp92II CATG 2 cut(s) 266, 344
HspAI GCGC 1 cut(s) 333
Kzo9I GATC 2 cut(s) 186, 390
LguI GCTCTTC 2 cut(s) 150, 164
LmnI GCTCC 1 cut(s) 52
Lsp1109I GCAGC 3 cut(s) 61, 183, 274
MaeI CTAG 1 cut(s) 384
MaeII ACGT 2 cut(s) 372, 403
MaeIII GTNAC 2 cut(s) 57, 352
MalI GATC 2 cut(s) 188, 392
MboI GATC 2 cut(s) 186, 390
MboII GAAGA 2 cut(s) 137, 181
MflI RGATCY 1 cut(s) 390
MhlI GDGCHC 1 cut(s) 176
MluCI AATT 1 cut(s) 153
MlyI GAGTC 1 cut(s) 114
MmeI TCCRAC 2 cut(s) 229, 286
MnlI CCTC 6 cut(s) 45, 95, 126, 286, 310, 381
MseI TTAA 1 cut(s) 409
MspI CCGG 2 cut(s) 36, 99
MspR9I CCNGG 2 cut(s) 132, 281
MvaI CCWGG 2 cut(s) 132, 281
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
NdeII GATC 2 cut(s) 186, 390
NlaIII CATG 2 cut(s) 266, 344
NlaIV GGNNCC 4 cut(s) 48, 136, 254, 392
NmuCI GTSAC 1 cut(s) 57
NruI TCGCGA 1 cut(s) 150
NspV TTCGAA 1 cut(s) 157
PciSI GCTCTTC 2 cut(s) 150, 164
PcsI WCGNNNNNNNCGW 3 cut(s) 147, 154, 214
Pfl23II CGTACG 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 3 cut(s) 76, 198, 289
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
Psp6I CCWGG 2 cut(s) 130, 279
PspGI CCWGG 2 cut(s) 130, 279
PspLI CGTACG 1 cut(s) 8
PspN4I GGNNCC 4 cut(s) 48, 136, 254, 392
PspPI GGNCC 1 cut(s) 244
PsuI RGATCY 1 cut(s) 390
RruI TCGCGA 1 cut(s) 150
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
SapI GCTCTTC 2 cut(s) 150, 164
SaqAI TTAA 1 cut(s) 409
SatI GCNGC 3 cut(s) 75, 197, 288
Sau3AI GATC 2 cut(s) 186, 390
Sau96I GGNCC 1 cut(s) 244
SchI GAGTC 1 cut(s) 114
ScrFI CCNGG 2 cut(s) 132, 281
SduI GDGCHC 1 cut(s) 176
SetI ASST 8 cut(s) 183, 198, 249, 289, 330, 375, 381, 406
SfuI TTCGAA 1 cut(s) 157
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 1 cut(s) 244
Sse9I AATT 1 cut(s) 153
SsiI CCGC 6 cut(s) 39, 121, 127, 190, 250, 290
SspMI CTAG 1 cut(s) 384
StyD4I CCNGG 2 cut(s) 130, 279
TaaI ACNGT 2 cut(s) 139, 312
TaiI ACGT 2 cut(s) 375, 406
TaqI TCGA 1 cut(s) 157
TasI AATT 1 cut(s) 153
Tru1I TTAA 1 cut(s) 409
Tru9I TTAA 1 cut(s) 409
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 1 cut(s) 57
TseI GCWGC 3 cut(s) 74, 196, 287
Tsp45I GTSAC 1 cut(s) 57
TspDTI ATGAA 1 cut(s) 329
TspGWI ACGGA 2 cut(s) 257, 383
TspRI CASTG 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 244
XapI RAATTY 1 cut(s) 153
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.