MD04G1104800.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013043
Physical Location & Seq
Reverse (-)
19228498 .. 19235290
6793 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1104800.v1.1.491

Sequence Viewer

Length: 2772 bp
ATGGAGGTTTCCCCTCTCCTTCTCTCTCTCCTCCTCCTCCTCATCCCCTCCTCTGCCCAAATGCCAGCAGGATTTGTGAGTTTGGATTGTGGAGGTGAAGAAATTTTCACTGATGATCTGGGTCTGGAGTGGACTCCTGATCAGATCAATTACGGCGAAACGGCCTTTATAGCTGTTTCAAATGAATCGAGGAAGCAATACTCGACGGTGAGGTACTTTCCGGCGGATCCGAGAAAATATTGTTACACATTGAATGTCACAAGTAGGACTAGGTACCTTCTAAGAGCGACATTCTTGTATGGTAACTTTGACACCAATAATGCTTATCCCAAATTTGACATTTCTGTTGGGGCAACGCATTGGTCTTCGATTGTAATTTCGGATGCCAATACGATAGAGATGCGAGAGCTTATATTTCTGGCTATGAGTCCGACTGTAAGTGTGTGTTTGTCCAATGCTACGACCGGACAGCCTTTTATATCGACTCTTGAGCTTCGACAATTCAATGGTTCTATGTATTATACTGAATTTGAGCAACAGTTCTATCTCAGTGTCTCCGCTAGGATTAATTTTGGTGCGGATGATGAAGCGCCAGTCAGGTATCCTGATGACCCCTTCGATAGAATATGGTTATCAGACTCTGTCAAGAAAGCAAATTACCTCGTCGACATTGCTGCTGGAACCAAAAAAGTGTCAACCAAGTTGCCAATTGACATTAATAGCGATGAAAGGCCGCCCGAAAAAGTTATGCAGACAGCTGTAGTTGGCACAAATGGAACACTGACTTACCGGCTGAACCTGGATGGTTTTCCTGGTTTTGGATGGGCATTTACCTACTTCGCAGAAATTGAAGATTTAGCTGAAGATGAGTCTAGAAAGTTTAGGCTAGTACTTCCGGGATATACTGAACTCAGCAAAGCTGTTGTTAATATTGAGGAAAATGCTCAAGGAAAATATCGTCTCTATGAACCAGGGTATACAAACTTATCCTTTCCGTTTGTATTATCGTTCAAATTTGGCAAAACATCCGATTCTTCCAGGGGGCCGCTCTTGAATGCAATGGAGATAAATAAATATCTGGAAATAAATGATGGTTCTCAAGATGGAGCTGTTATTTCCAGTTTTGTTTCACACTACTCATCAGCAGATTGGACTTTGGAAGGCGGTGATCCATGCCTCCCAGTTCCATGGTCTTGGGTTGAGTGTAACTCAAATCCACAACCAAGAATAGTTAAAATAAAATTGTCTAGTAAGAATATTACCGGGAATATCCCTTCAGACTTGACAAAGTTGAACGGTTTAGAAGAGTTATGGCTTGATGGGAACTCACTGACTGGTACAATACCTGATTTCACTGGATGTGTGGACTTGAAGATTATTCATCTTGAGAACAATCAGTTGACGGGTGGACTCTCCTCTTTCACAAACCTACCAAGTTTGAAGGAACTGTATGTTCAAAATAATATGTTAAGCGGAACAGTGCCATCGGGTCTTCTCGATAAAGTGGTTAATATGAACTACACTGGAAATGTTAATCTTCGGAAAGGGAAAACAAGTGGGAGCCGCACAAACATTATTATTGGTACGTCAGTTGGGGCTGCTGTCTTGGTCATAGCTACTATTGTATCTGGCGTACTTTTACGGAAGGGGAGGAAGAAATGTTACGAGCATGACCAACTTGGGCATCCACCTGCGCAAGGAATACATTTTTCTAAGTGTGATGCTCCTAATGAATCTGCACACTGCTTCTCTTATGCCGAAATTGAAGAGGCTACTAGAAAGTTTGAGAAGAAAATAGGTTCCGGAGGTTTTGGAGTTGTTTACTACGGTAAAATGAAGGATGAAAAGGAAATTGCTGTCAAAGTTCTAACCAGCAATTCCTACCAGGGAAAGCGAGAATTTTCAAATGAGGTAACTCTTCTTTCGAGGATACATCACAGAAACCTGGTACAGTTTCTTGGGTATTGCCAAGAGGATGGAAGAAGTATGCTGATTTACGAGTTTATGCATAATGGAACTCTCAAGGAACATCTTTATGGCCCATTAACACACGAACAGAGTATCAATTGGATCAAACGCCTTGAGATTGCTGAAGATGCTGCAAAAGGAATCGAATACCTTCACACAGGCTGTGTTCCAGCCATCATTCATAGAGATTTAAAAAGCAGCAACATTTTAATTGACAAACACATGAGAGCAAAGGTTTCAGATTTTGGTCTTTCCAAACTTGCAGTAGATGGGGCATCCCATGTGTCAAGCATAGTTCGGGGCACTGTAGGGTATCTGGATCCTGAGTATTACATCTCCCAGCAGTTGACAGACAAGAGTGACGTATACAGTTTCGGTGTCATTCTGCTTGAGCTGATATCAGGTCAAGAAGCGATATCTAATGAAAACTTTGGTGTTAACTGCCGTAACATAGTGCAATGGGCAAAATTACACATTGAGAGTGGCGACATTCAAGGAATAATCGACCCCTCACTGGATGGCGAATACGACATCCAGTCAATGTGGAAGATAGCAGAAAAAGCCTTAATGTGTGTTCAAGCACACGGATTCATGAGGCCGTCCATGTCAGAAGTTCTCAAAGAAATCCAAGATGCAATCTCAATGGAAAGGGATGCCGGAGCAGTAAGAGAAGGTAACTCAGATATATCGAGGAATTCAATCCATTCAATTCATTCTTCCTTGAACATGGGTTCCCTGGACCTTGGCAGAAATGATAATTTCTTGTCGATCGATGAGTCTATCGCACGGCCAACTGCTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

924

Amino Acids

102.57

Weight (kDa)

5.4

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 28 - 357 1.3e-75 Malectin-like domain
PK_Tyr_Ser-Thr PF07714 594 - 795 5.1e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 595 - 813 3.7e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013043)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37050 AT2G37050 AT2G37050 AT2G37050
fragaria_vesca FvH4_6g15210
malus_domestica MD04G1104800.v1.1 MD12G1125100.v1.1
prunus_persica Prupe.6G241900_v2.0.a1 Prupe.6G241900_v2.0.a1
pyrus_communis pycom12g12330
rosa_chinensis RchiOBHm_Chr3g0468561
rosa_laevigata RLG00000024422
rosa_multiflora Rmu_sc0009516.1_g000006
rosa_roxburghii Rroxscaffold_6G00412690
rosa_rugosa Rorug03G0096000
rosa_samantha Rh3BG166600 Rh3DG166000
rosa_wichuraiana Rw3G013630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1699
Acc16I TGCGCA 1 cut(s) 1695
Acc36I ACCTGC 1 cut(s) 1699
Acc65I GGTACC 1 cut(s) 273
AccB1I GGYRCC 1 cut(s) 273
AccBSI CCGCTC 1 cut(s) 1048
AccI GTMKAC 3 cut(s) 666, 977, 2334
AccIII TCCGGA 1 cut(s) 1802
AciI CCGC 8 cut(s) 224, 558, 578, 734, 1046, 1164, 1473, 1564
AclWI GGATC 6 cut(s) 221, 234, 1163, 2078, 2282, 2295
AcoI YGGCCR 1 cut(s) 2756
AcsI RAATTY 6 cut(s) 102, 332, 527, 1013, 1898, 2662
AcuI CTGAAG 3 cut(s) 882, 1260, 2112
AfaI GTAC 7 cut(s) 215, 275, 891, 1339, 1585, 1635, 1950
AfiI CCNNNNNNNGG 3 cut(s) 818, 1697, 2482
AhdI GACNNNNNGTC 1 cut(s) 2503
AjnI CCWGG 7 cut(s) 798, 811, 970, 1037, 1884, 1944, 2703
AloI GAACNNNNNNTCC 2 cut(s) 2684, 2716
AluBI AGCT 9 cut(s) 173, 409, 493, 758, 860, 920, 1109, 1616, 2362
AluI AGCT 9 cut(s) 173, 409, 493, 758, 860, 920, 1109, 1616, 2362
Alw26I GTCTC 2 cut(s) 559, 965
AlwI GGATC 6 cut(s) 221, 234, 1163, 2078, 2282, 2295
AlwNI CAGNNNCTG 1 cut(s) 641
Aor13HI TCCGGA 1 cut(s) 1802
AoxI GGCC 6 cut(s) 162, 731, 1043, 2038, 2564, 2756
ApeKI GCWGC 4 cut(s) 674, 1598, 2099, 2166
ApoI RAATTY 6 cut(s) 102, 332, 527, 1013, 1898, 2662
AseI ATTAAT 2 cut(s) 567, 717
Asp700I GAANNNNTTC 1 cut(s) 2118
Asp718I GGTACC 1 cut(s) 273
AspLEI GCGC 2 cut(s) 592, 1696
AspS9I GGNCC 3 cut(s) 1043, 2039, 2707
AsuC2I CCSGG 2 cut(s) 897, 1264
AsuHPI GGTGA 3 cut(s) 107, 220, 1178
AvaII GGWCC 1 cut(s) 2707
BaeGI GKGCMC 1 cut(s) 2273
BaeI ACNNNNGTAYC 4 cut(s) 1575, 1608, 1608, 1641
BamHI GGATCC 2 cut(s) 226, 2287
BanI GGYRCC 1 cut(s) 273
BarI GAAGNNNNNNTAC 2 cut(s) 1646, 1678
BbsI GAAGAC 2 cut(s) 357, 1484
BbvI GCAGC 4 cut(s) 661, 1585, 2086, 2178
BceAI ACGGC 4 cut(s) 169, 177, 2397, 2551
BcgI CGANNNNNNTGC 4 cut(s) 382, 416, 656, 690
BciT130I CCWGG 7 cut(s) 800, 813, 972, 1039, 1886, 1946, 2705
BciVI GTATCC 2 cut(s) 612, 1923
BclI TGATCA 1 cut(s) 139
BcnI CCSGG 2 cut(s) 897, 1264
BcoDI GTCTC 2 cut(s) 559, 965
BfaI CTAG 6 cut(s) 270, 561, 873, 887, 1248, 1776
BfmI CTRYAG 2 cut(s) 759, 2274
BfoI RGCGCY 1 cut(s) 593
BfuAI ACCTGC 1 cut(s) 1699
BfuI GTATCC 2 cut(s) 612, 1923
BisI GCNGC 7 cut(s) 675, 734, 1046, 1564, 1599, 2100, 2167
BlsI GCNGC 7 cut(s) 676, 735, 1047, 1565, 1600, 2101, 2168
BmcAI AGTACT 1 cut(s) 891
Bme1390I CCNGG 9 cut(s) 800, 813, 897, 972, 1039, 1264, 1886, 1946, 2705
Bme18I GGWCC 1 cut(s) 2707
BmeRI GACNNNNNGTC 1 cut(s) 2503
BmgT120I GGNCC 3 cut(s) 1043, 2039, 2707
BmiI GGNNCC 8 cut(s) 228, 275, 682, 1044, 1562, 1801, 2289, 2701
BmrFI CCNGG 9 cut(s) 800, 813, 897, 972, 1039, 1264, 1886, 1946, 2705
BmrI ACTGGG 1 cut(s) 1175
BmsI GCATC 8 cut(s) 373, 390, 1693, 1711, 2086, 2252, 2590, 2611
BmuI ACTGGG 1 cut(s) 1175
BpiI GAAGAC 2 cut(s) 357, 1484
BplI GAGNNNNNCTC 2 cut(s) 1193, 1225
BpmI CTGGAG 1 cut(s) 146
BpuEI CTTGAG 7 cut(s) 509, 930, 1083, 1406, 2006, 2102, 2378
BpuMI CCSGG 2 cut(s) 897, 1264
Bsa29I ATCGAT 1 cut(s) 2739
BsaJI CCNNGG 6 cut(s) 971, 1038, 1187, 1885, 2703, 2710
BsaWI WCCGGW 2 cut(s) 464, 1802
Bsc4I CCNNNNNNNGG 3 cut(s) 818, 1697, 2482
Bse118I RCCGGY 1 cut(s) 789
Bse1I ACTGG 8 cut(s) 593, 1119, 1181, 1339, 1360, 1528, 2487, 2503
Bse3DI GCAATG 3 cut(s) 669, 1065, 2432
BseAI TCCGGA 1 cut(s) 1802
BseBI CCWGG 7 cut(s) 800, 813, 972, 1039, 1886, 1946, 2705
BseCI ATCGAT 1 cut(s) 2739
BseDI CCNNGG 6 cut(s) 971, 1038, 1187, 1885, 2703, 2710
BseLI CCNNNNNNNGG 3 cut(s) 818, 1697, 2482
BseMI GCAATG 3 cut(s) 669, 1065, 2432
BseMII CTCAG 4 cut(s) 562, 925, 2283, 2661
BseNI ACTGG 8 cut(s) 593, 1119, 1181, 1339, 1360, 1528, 2487, 2503
BseRI GAGGAG 6 cut(s) 20, 23, 26, 29, 40, 1405
BseSI GKGCMC 1 cut(s) 2273
BseXI GCAGC 4 cut(s) 661, 1585, 2086, 2178
BseYI CCCAGC 1 cut(s) 2307
BsgI GTGCAG 1 cut(s) 1722
Bsh1285I CGRYCG 2 cut(s) 465, 2739
BshFI GGCC 6 cut(s) 164, 733, 1045, 2040, 2566, 2758
BshNI GGYRCC 1 cut(s) 273
BshVI ATCGAT 1 cut(s) 2739
BsiEI CGRYCG 2 cut(s) 465, 2739
BsiSI CCGG 7 cut(s) 221, 465, 790, 896, 1263, 1803, 2625
BslI CCNNNNNNNGG 3 cut(s) 818, 1697, 2482
BsmAI GTCTC 2 cut(s) 559, 965
BsmBI CGTCTC 1 cut(s) 965
BsmI GAATGC 1 cut(s) 1060
BsnI GGCC 6 cut(s) 164, 733, 1045, 2040, 2566, 2758
Bsp1286I GDGCHC 1 cut(s) 2273
Bsp13I TCCGGA 1 cut(s) 1802
Bsp143I GATC 8 cut(s) 115, 139, 144, 226, 1168, 2070, 2287, 2736
Bsp19I CCATGG 1 cut(s) 1187
BspACI CCGC 8 cut(s) 224, 558, 578, 734, 1046, 1164, 1473, 1564
BspANI GGCC 6 cut(s) 164, 733, 1045, 2040, 2566, 2758
BspCNI CTCAG 4 cut(s) 561, 924, 2284, 2660
BspDI ATCGAT 1 cut(s) 2739
BspEI TCCGGA 1 cut(s) 1802
BspHI TCATGA 1 cut(s) 2559
BspLI GGNNCC 8 cut(s) 228, 275, 682, 1044, 1562, 1801, 2289, 2701
BspMI ACCTGC 1 cut(s) 1699
BspPI GGATC 6 cut(s) 221, 234, 1163, 2078, 2282, 2295
BspT107I GGYRCC 1 cut(s) 273
BsrBI CCGCTC 1 cut(s) 1048
BsrDI GCAATG 3 cut(s) 669, 1065, 2432
BsrFI RCCGGY 1 cut(s) 789
BsrI ACTGG 8 cut(s) 593, 1119, 1181, 1339, 1360, 1528, 2487, 2503
BssAI RCCGGY 1 cut(s) 789
BssECI CCNNGG 6 cut(s) 971, 1038, 1187, 1885, 2703, 2710
BssMI GATC 8 cut(s) 115, 139, 144, 226, 1168, 2070, 2287, 2736
BssNAI GTATAC 2 cut(s) 978, 2335
BssT1I CCWWGG 2 cut(s) 1187, 2710
Bst1107I GTATAC 2 cut(s) 978, 2335
Bst2UI CCWGG 7 cut(s) 800, 813, 972, 1039, 1886, 1946, 2705
Bst6I CTCTTC 3 cut(s) 1299, 1761, 1923
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 6 cut(s) 281, 548, 911, 1713, 2292, 2647
BstDSI CCRYGG 1 cut(s) 1187
BstENI CCTNNNNNAGG 1 cut(s) 1695
BstH2I RGCGCY 1 cut(s) 593
BstHHI GCGC 2 cut(s) 592, 1696
BstKTI GATC 8 cut(s) 118, 142, 147, 229, 1171, 2073, 2290, 2739
BstMAI GTCTC 2 cut(s) 559, 965
BstMBI GATC 8 cut(s) 115, 139, 144, 226, 1168, 2070, 2287, 2736
BstMCI CGRYCG 2 cut(s) 465, 2739
BstMWI GCNNNNNNNGC 3 cut(s) 170, 2096, 2528
BstNI CCWGG 7 cut(s) 800, 813, 972, 1039, 1886, 1946, 2705
BstSCI CCNGG 9 cut(s) 798, 811, 895, 970, 1037, 1262, 1884, 1944, 2703
BstSFI CTRYAG 2 cut(s) 759, 2274
BstSLI GKGCMC 1 cut(s) 2273
BstV1I GCAGC 4 cut(s) 661, 1585, 2086, 2178
BstV2I GAAGAC 2 cut(s) 357, 1484
BstX2I RGATCY 2 cut(s) 226, 2287
BstXI CCANNNNNNTGG 3 cut(s) 1188, 1194, 1976
BstYI RGATCY 2 cut(s) 226, 2287
BstZ17I GTATAC 2 cut(s) 978, 2335
Bsu15I ATCGAT 1 cut(s) 2739
BsuI GTATCC 2 cut(s) 612, 1923
BsuRI GGCC 6 cut(s) 164, 733, 1045, 2040, 2566, 2758
BsuTUI ATCGAT 1 cut(s) 2739
BtgI CCRYGG 1 cut(s) 1187
BtgZI GCGATG 1 cut(s) 738
BtsI GCAGTG 1 cut(s) 1741
BveI ACCTGC 1 cut(s) 1699
Cac8I GCNNGC 1 cut(s) 66
CaiI CAGNNNCTG 1 cut(s) 641
CciI TCATGA 1 cut(s) 2559
CfoI GCGC 2 cut(s) 592, 1696
Cfr10I RCCGGY 1 cut(s) 789
Cfr13I GGNCC 3 cut(s) 1043, 2039, 2707
ClaI ATCGAT 1 cut(s) 2739
CsiI ACCWGGT 1 cut(s) 1944
Csp6I GTAC 7 cut(s) 214, 274, 890, 1338, 1584, 1634, 1949
CviAII CATG 8 cut(s) 1173, 1188, 1670, 2191, 2249, 2560, 2572, 2695
CviQI GTAC 7 cut(s) 214, 274, 890, 1338, 1584, 1634, 1949
DdeI CTNAG 6 cut(s) 281, 548, 911, 1713, 2292, 2647
DpnI GATC 8 cut(s) 117, 141, 146, 228, 1170, 2072, 2289, 2738
DpnII GATC 8 cut(s) 115, 139, 144, 226, 1168, 2070, 2287, 2736
DraI TTTAAA 1 cut(s) 2160
DriI GACNNNNNGTC 1 cut(s) 2503
EaeI YGGCCR 1 cut(s) 2756
Eam1104I CTCTTC 3 cut(s) 1299, 1761, 1923
Eam1105I GACNNNNNGTC 1 cut(s) 2503
EarI CTCTTC 3 cut(s) 1299, 1761, 1923
EciI GGCGGA 1 cut(s) 239
Eco130I CCWWGG 2 cut(s) 1187, 2710
Eco32I GATATC 2 cut(s) 2367, 2385
Eco47I GGWCC 1 cut(s) 2707
Eco57I CTGAAG 3 cut(s) 882, 1260, 2112
EcoNI CCTNNNNNAGG 1 cut(s) 1695
EcoRI GAATTC 1 cut(s) 2662
EcoRII CCWGG 7 cut(s) 798, 811, 970, 1037, 1884, 1944, 2703
EcoRV GATATC 2 cut(s) 2367, 2385
EcoT14I CCWWGG 2 cut(s) 1187, 2710
EcoT22I ATGCAT 1 cut(s) 2010
ErhI CCWWGG 2 cut(s) 1187, 2710
Esp3I CGTCTC 1 cut(s) 965
FaeI CATG 8 cut(s) 1176, 1191, 1673, 2194, 2252, 2563, 2575, 2698
FatI CATG 8 cut(s) 1172, 1187, 1669, 2190, 2248, 2559, 2571, 2694
FbaI TGATCA 1 cut(s) 139
FblI GTMKAC 3 cut(s) 666, 977, 2334
Fnu4HI GCNGC 7 cut(s) 675, 734, 1046, 1564, 1599, 2100, 2167
Fsp4HI GCNGC 7 cut(s) 675, 734, 1046, 1564, 1599, 2100, 2167
FspBI CTAG 6 cut(s) 270, 561, 873, 887, 1248, 1776
FspI TGCGCA 1 cut(s) 1695
GlaI GCGC 2 cut(s) 591, 1695
GluI GCNGC 7 cut(s) 675, 734, 1046, 1564, 1599, 2100, 2167
GsaI CCCAGC 1 cut(s) 2311
GsuI CTGGAG 1 cut(s) 146
HaeII RGCGCY 1 cut(s) 593
HaeIII GGCC 6 cut(s) 164, 733, 1045, 2040, 2566, 2758
HapII CCGG 7 cut(s) 221, 465, 790, 896, 1263, 1803, 2625
HhaI GCGC 2 cut(s) 592, 1696
Hin1II CATG 8 cut(s) 1176, 1191, 1673, 2194, 2252, 2563, 2575, 2698
Hin6I GCGC 2 cut(s) 590, 1694
HinP1I GCGC 2 cut(s) 590, 1694
HincII GTYRAC 5 cut(s) 667, 696, 1401, 2316, 2407
HindII GTYRAC 5 cut(s) 667, 696, 1401, 2316, 2407
HpaI GTTAAC 1 cut(s) 2407
HpaII CCGG 7 cut(s) 221, 465, 790, 896, 1263, 1803, 2625
HphI GGTGA 3 cut(s) 107, 220, 1178
Hpy99I CGWCG 2 cut(s) 208, 668
HpyCH4IV ACGT 2 cut(s) 1586, 2331
HpyCH4V TGCA 8 cut(s) 751, 1058, 1739, 2008, 2102, 2231, 2425, 2603
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 2096, 2528
HpyF3I CTNAG 6 cut(s) 281, 548, 911, 1713, 2292, 2647
HpySE526I ACGT 2 cut(s) 1586, 2331
Hsp92II CATG 8 cut(s) 1176, 1191, 1673, 2194, 2252, 2563, 2575, 2698
HspAI GCGC 2 cut(s) 590, 1694
Kpn2I TCCGGA 1 cut(s) 1802
KpnI GGTACC 1 cut(s) 277
Ksp22I TGATCA 1 cut(s) 139
KspAI GTTAAC 1 cut(s) 2407
Kzo9I GATC 8 cut(s) 115, 139, 144, 226, 1168, 2070, 2287, 2736
LmnI GCTCC 4 cut(s) 1106, 1560, 1729, 2627
Lsp1109I GCAGC 4 cut(s) 661, 1585, 2086, 2178
LweI GCATC 8 cut(s) 373, 390, 1693, 1711, 2086, 2252, 2590, 2611
MabI ACCWGGT 1 cut(s) 1944
MaeI CTAG 6 cut(s) 270, 561, 873, 887, 1248, 1776
MaeII ACGT 2 cut(s) 1586, 2331
MaeIII GTNAC 9 cut(s) 242, 256, 302, 1205, 1661, 1912, 2327, 2414, 2642
MalI GATC 8 cut(s) 117, 141, 146, 228, 1170, 2072, 2289, 2738
MbiI CCGCTC 1 cut(s) 1048
MboI GATC 8 cut(s) 115, 139, 144, 226, 1168, 2070, 2287, 2736
MfeI CAATTG 2 cut(s) 708, 2065
MflI RGATCY 2 cut(s) 226, 2287
MhlI GDGCHC 1 cut(s) 2273
MlyI GAGTC 7 cut(s) 127, 436, 478, 632, 878, 1404, 2753
MmeI TCCRAC 1 cut(s) 455
Mph1103I ATGCAT 1 cut(s) 2010
MroI TCCGGA 1 cut(s) 1802
MroXI GAANNNNTTC 1 cut(s) 2118
MslI CAYNNNNRTG 1 cut(s) 2034
MspA1I CMGCKG 1 cut(s) 758
MspI CCGG 7 cut(s) 221, 465, 790, 896, 1263, 1803, 2625
MspR9I CCNGG 9 cut(s) 800, 813, 897, 972, 1039, 1264, 1886, 1946, 2705
MunI CAATTG 2 cut(s) 708, 2065
Mva1269I GAATGC 1 cut(s) 1060
MvaI CCWGG 7 cut(s) 800, 813, 972, 1039, 1886, 1946, 2705
MwoI GCNNNNNNNGC 3 cut(s) 170, 2096, 2528
NciI CCSGG 2 cut(s) 897, 1264
NcoI CCATGG 1 cut(s) 1187
NdeII GATC 8 cut(s) 115, 139, 144, 226, 1168, 2070, 2287, 2736
NlaIII CATG 8 cut(s) 1176, 1191, 1673, 2194, 2252, 2563, 2575, 2698
NlaIV GGNNCC 8 cut(s) 228, 275, 682, 1044, 1562, 1801, 2289, 2701
NmuCI GTSAC 2 cut(s) 256, 2327
NsbI TGCGCA 1 cut(s) 1695
NsiI ATGCAT 1 cut(s) 2010
PagI TCATGA 1 cut(s) 2559
PaqCI CACCTGC 1 cut(s) 1699
PctI GAATGC 1 cut(s) 1060
PdmI GAANNNNTTC 1 cut(s) 2118
PfeI GAWTC 5 cut(s) 185, 1031, 1733, 2109, 2556
PflFI GACNNNGTC 1 cut(s) 641
PfoI TCCNGGA 1 cut(s) 895
PkrI GCNGC 7 cut(s) 676, 735, 1047, 1565, 1600, 2101, 2168
Ple19I CGATCG 1 cut(s) 2739
PleI GAGTC 7 cut(s) 127, 435, 478, 632, 877, 1404, 2752
PpsI GAGTC 7 cut(s) 127, 435, 478, 632, 877, 1404, 2752
PshBI ATTAAT 2 cut(s) 567, 717
Psp6I CCWGG 7 cut(s) 798, 811, 970, 1037, 1884, 1944, 2703
PspFI CCCAGC 1 cut(s) 2307
PspGI CCWGG 7 cut(s) 798, 811, 970, 1037, 1884, 1944, 2703
PspN4I GGNNCC 8 cut(s) 228, 275, 682, 1044, 1562, 1801, 2289, 2701
PspPI GGNCC 3 cut(s) 1043, 2039, 2707
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 2 cut(s) 226, 2287
PsyI GACNNNGTC 1 cut(s) 641
PvuI CGATCG 1 cut(s) 2739
PvuII CAGCTG 1 cut(s) 758
RsaI GTAC 7 cut(s) 215, 275, 891, 1339, 1585, 1635, 1950
RsaNI GTAC 7 cut(s) 214, 274, 890, 1338, 1584, 1634, 1949
RseI CAYNNNNRTG 1 cut(s) 2034
SalI GTCGAC 1 cut(s) 665
SatI GCNGC 7 cut(s) 675, 734, 1046, 1564, 1599, 2100, 2167
Sau3AI GATC 8 cut(s) 115, 139, 144, 226, 1168, 2070, 2287, 2736
Sau96I GGNCC 3 cut(s) 1043, 2039, 2707
ScaI AGTACT 1 cut(s) 891
SchI GAGTC 7 cut(s) 127, 436, 478, 632, 878, 1404, 2753
ScrFI CCNGG 9 cut(s) 800, 813, 897, 972, 1039, 1264, 1886, 1946, 2705
SduI GDGCHC 1 cut(s) 2273
SexAI ACCWGGT 1 cut(s) 1944
SfaNI GCATC 8 cut(s) 373, 390, 1693, 1711, 2086, 2252, 2590, 2611
SfcI CTRYAG 2 cut(s) 759, 2274
SinI GGWCC 1 cut(s) 2707
SmiMI CAYNNNNRTG 1 cut(s) 2034
SmlI CTYRAG 7 cut(s) 488, 945, 1098, 1385, 2021, 2081, 2357
SmoI CTYRAG 7 cut(s) 488, 945, 1098, 1385, 2021, 2081, 2357
SsiI CCGC 8 cut(s) 224, 558, 578, 734, 1046, 1164, 1473, 1564
SspI AATATT 3 cut(s) 239, 931, 1258
SspMI CTAG 6 cut(s) 270, 561, 873, 887, 1248, 1776
StyD4I CCNGG 9 cut(s) 798, 811, 895, 970, 1037, 1262, 1884, 1944, 2703
StyI CCWWGG 2 cut(s) 1187, 2710
TaiI ACGT 2 cut(s) 1589, 2334
TatI WGTACW 1 cut(s) 889
TauI GCSGC 3 cut(s) 736, 1048, 1566
TfiI GAWTC 5 cut(s) 185, 1031, 1733, 2109, 2556
TseFI GTSAC 2 cut(s) 256, 2327
TseI GCWGC 4 cut(s) 674, 1598, 2099, 2166
Tsp45I GTSAC 2 cut(s) 256, 2327
TspGWI ACGGA 3 cut(s) 984, 1657, 2568
Tth111I GACNNNGTC 1 cut(s) 641
VpaK11BI GGWCC 1 cut(s) 2707
VspI ATTAAT 2 cut(s) 567, 717
XagI CCTNNNNNAGG 1 cut(s) 1695
XapI RAATTY 6 cut(s) 102, 332, 527, 1013, 1898, 2662
XbaI TCTAGA 1 cut(s) 872
XmiI GTMKAC 3 cut(s) 666, 977, 2334
XmnI GAANNNNTTC 1 cut(s) 2118
XspI CTAG 6 cut(s) 270, 561, 873, 887, 1248, 1776
ZrmI AGTACT 1 cut(s) 891
Zsp2I ATGCAT 1 cut(s) 2010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.