MD12G1125100.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013043
Physical Location & Seq
Reverse (-)
20009994 .. 20016301
6308 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1125100.v1.1.491

Sequence Viewer

Length: 2757 bp
ATGGAGGTTTCCCTTCTCCTTCTCTCTCTCCTCCTTCTCGTCGTCCCTTCCTCTGCTCAAATGCCAGGGTTTGTGAGTTTGGATTGTGGAGGTGAAGAAATTTTCACTGATGATCTGGGTGTGAAGTGGTCTCCTGATCAGCTCAGTTTCGGTGAGACGTCCTCTATAGCTGTTTCCAATGAGACCAGGAAGCAGTACTCGACGTTGAGGCATTTTCCGGCGGATTCGAGAAAATATTGTTACACATTGAATGTCACAAGTAGGACTAGGTACCTTCTAAGAGCGACATTCTTGTATGGTAACTTTGACAGCAATAACGTTTATCCCAAATTTGACATTTCTGTTGGGGCAACCCATTGGTCTTCGATTGTAATTTCGGATGCCAATACGATGGAGATGCGCGAGCTTATATTTCTGGCGATGAGTCCTACTGTCAGTGTATGTTTATCCAATGCTACGACTGGACAACCTTTTATATCCACTCTTGAGCTTCGACAATTCAATGGTTCCATGTACTATTCTGAGTATGAGCAACAGTTCTATCTCAGTGTCTCTGCCAGGATTAATTTCGGTGCAGATGATGAAGCACCAGTCAGGTATCCTGATGACCCTTTCGATAGAATATGGACATCAGACTCTGTCAAGAAAGCGAATTACCTCGTCGACATTGCTGCTGGAACCAAAAAAGTGTCAACCAAGTTGCCAATTGACATTAATAGGGATGACAGGCCACCCGAAAAAGTTATGCAGACAGCTGTAGTTGGCACAAATGGAACACTGACTTACCGGTTGAACCTGGATGGTTTTCCTGGTTTTGGATGGGCATGCACCTACTTAGCAGAAATTGAAGATTTAGCTGAAGATGAGTCTAGAAAGTTTAGGCTAGTACTTCCAGGAAATACTGAACTCAGCAAAGCTGTTGTTAATATTGAAGAAAACGCTCAGGGAAAATATCGTCTCTATGAACCAGGGTTAACAAACTTATCCTTTCCATTTGTATTATCATTTAAATTTGCCAAAACAGCCGATTCTTCCAGGGGACCGCTCTTGAATGCAATGGAGATAAATAAATATCTGGAAATAAATGATGGTTCTCAAGATGGAGCTGTTATTTCTAGTTTTGTTTCACACTACTCATCGGCAGATTGGACTTTGGAAGGCGGTGATCCATGCCTCCCAGTATCATGGTCTTGGGTTGAGTGTAACTCAGATCCACAACCAAGAATAGTTAAAATAAAATTATCTAGTAAGAATATTACTGGGAATATCCCTTCAGACTTGACAAAGTTGAACAGTTTAGAAGAGTTATGGCTTGACGGGAACTCACTGACTGGTACAATACCTGATTTCACTGGATGTGTGGACTTGAAGATTATTCATCTTGAAAACAATCAGTTGACGGGCGGACTCTCCTCTTTCACGAACCTACCAAGTTTGAAGGAACTGTATGTGCAAAATAATATGTTAACCGGAACTGTGCCATCAAGTCTTCTCGATAAAGTGGTTAATATGAACTACACTGGAAATGTTAATCTGCGGAAAGGGAAAACAAGCGGGAGCCGCAATAACATTATTATTGGTACATCAGTTGGGTCTGCTGTCTTGGTCATAGCTACTATTGTATCTTGCATACTTTTACGCAAGGGGAGGAAGAAATACAAGCATGACCAACTTGGGAATCAACCTGCGCAAGGAATACATTCTTCTAAGAGTGATGCTCCTAAAGAATCTGCACACTGCTTCTCTTACTCCGAAATTGAAGAGGCTACCAGAAAGTTTGAGAAGAAAATAGGTTCGGGAGGTTTTGGAGTTGTTTACTACGGTATAATGAAGGATGAAAAGGAAATTGCAGTCAAAGTTCTAACGAGTAATTCCTACCAGGGAAAGCGAGAATTTTCAAATGAGGTAACTCTTCTTTCAAGGATACATCACAGACACCTGGTACAGTTTCTTGGGTATTGCCAAGAGGATGGAAGAAGTATGCTGATCTACGAGTTCATGCATAATGGAACTCTCAAGGAACATCTTTATGGCCCATTAACACACGAACAGAGTATCAATTGGATCAAGCGCCTTGAGATTGCTGAAGATGCTGCAAAAGGAATCGAATACCTTCACACCGGCTGTGTTCCAGCTATCATTCATAGAGATTTGAAAAGCAGCAACATTCTAATTGACAAACACATGAGAGCAAAGGTTTCAGATTTTGGTCTTTCCAAACTTGAAGTAGAAGGAGCATCCCATGTGTCAAGCAAAGTTCGGGGCACTGTAGGGTATCTGGATCCCGAGTATTACATCTCCCAGCAGTTGACAGACAAGAGTGACGTTTATAGTTTCGGTGTCATTCTTCTTGAGCTGATATCAGGTCAAGAAGCGATTTCTAATGAAAACTTTGGTGTTAACTGCCGTAATATAGTGCAATGGGCAAAATTACACATCGAGAATGGCAACATTCAAGGAATAATCGACCCCTCGCTGGATGGCGAATACGACATCCAGTCAATGTGGAAGATAGCCGAAAAAGCCCTAATGTGTGTTCTAGCGCACGGATTTATGAGGCCGTCCATTTCAGAAGTTCTCAAGGAAATCCAAGATGCAATCTCAGTGGAAAGGGATGCTGCAGCAGTAAGAGAAGGTTCCTCAGATGTATCAAAGAATTCAATCCATTCTTCCTTGAACACAGGTTCCCTGGATCTTGGCAGAAATGACAATTTCTTGTCGATCGATGAGTCTATCTCCCGGCCAACTGCTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

919

Amino Acids

101.93

Weight (kDa)

5.62

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 27 - 356 1.6e-73 Malectin-like domain
PK_Tyr_Ser-Thr PF07714 592 - 792 5.3e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 593 - 811 5.8e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013043)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37050 AT2G37050 AT2G37050 AT2G37050
fragaria_vesca FvH4_6g15210
malus_domestica MD04G1104800.v1.1 MD12G1125100.v1.1
prunus_persica Prupe.6G241900_v2.0.a1 Prupe.6G241900_v2.0.a1
pyrus_communis pycom12g12330
rosa_chinensis RchiOBHm_Chr3g0468561
rosa_laevigata RLG00000024422
rosa_multiflora Rmu_sc0009516.1_g000006
rosa_roxburghii Rroxscaffold_6G00412690
rosa_rugosa Rorug03G0096000
rosa_samantha Rh3BG166600 Rh3DG166000
rosa_wichuraiana Rw3G013630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 161
Acc16I TGCGCA 1 cut(s) 1689
Acc36I ACCTGC 1 cut(s) 1693
Acc65I GGTACC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 1045
AccI GTMKAC 1 cut(s) 663
AccII CGCG 1 cut(s) 402
AciI CCGC 7 cut(s) 221, 1043, 1161, 1404, 1537, 1554, 1561
AclI AACGTT 1 cut(s) 318
AclWI GGATC 6 cut(s) 1160, 1205, 2072, 2276, 2289, 2700
AcoI YGGCCR 1 cut(s) 2741
AcsI RAATTY 5 cut(s) 99, 329, 1010, 1892, 2656
AcuI CTGAAG 3 cut(s) 879, 1257, 2106
AcyI GRCGYC 1 cut(s) 158
AfaI GTAC 7 cut(s) 197, 272, 515, 888, 1336, 1582, 1944
AfiI CCNNNNNNNGG 3 cut(s) 815, 1691, 2476
AgeI ACCGGT 1 cut(s) 786
AhdI GACNNNNNGTC 1 cut(s) 2497
AloI GAACNNNNNNTCC 2 cut(s) 2669, 2701
Alw26I GTCTC 5 cut(s) 135, 149, 176, 556, 962
AlwI GGATC 6 cut(s) 1160, 1205, 2072, 2276, 2289, 2700
AlwNI CAGNNNCTG 1 cut(s) 638
Ama87I CYCGRG 1 cut(s) 2285
AoxI GGCC 4 cut(s) 728, 2032, 2558, 2741
ApeKI GCWGC 5 cut(s) 671, 2093, 2160, 2618, 2621
ApoI RAATTY 5 cut(s) 99, 329, 1010, 1892, 2656
AseI ATTAAT 2 cut(s) 564, 714
AsiGI ACCGGT 1 cut(s) 786
Asp700I GAANNNNTTC 2 cut(s) 1699, 2112
Asp718I GGTACC 1 cut(s) 270
AspLEI GCGC 4 cut(s) 402, 1690, 2073, 2545
AspS9I GGNCC 2 cut(s) 1040, 2033
AsuC2I CCSGG 1 cut(s) 2740
AsuHPI GGTGA 3 cut(s) 104, 164, 1175
AvaI CYCGRG 1 cut(s) 2285
AvaII GGWCC 1 cut(s) 1040
BaeGI GKGCMC 1 cut(s) 2267
BaeI ACNNNNGTAYC 4 cut(s) 1572, 1605, 1605, 1638
BamHI GGATCC 1 cut(s) 2281
BanI GGYRCC 1 cut(s) 270
BbsI GAAGAC 2 cut(s) 354, 1481
BbvI GCAGC 5 cut(s) 658, 2080, 2172, 2605, 2633
BccI CCATC 8 cut(s) 385, 794, 813, 1082, 1094, 1489, 1964, 2474
BceAI ACGGC 2 cut(s) 2391, 2545
BcgI CGANNNNNNTGC 4 cut(s) 379, 413, 653, 687
BciVI GTATCC 2 cut(s) 609, 1917
BclI TGATCA 1 cut(s) 136
BcnI CCSGG 1 cut(s) 2740
BcoDI GTCTC 5 cut(s) 135, 149, 176, 556, 962
BfaI CTAG 6 cut(s) 267, 870, 884, 1116, 1245, 2540
BfmI CTRYAG 4 cut(s) 165, 756, 2268, 2619
BfoI RGCGCY 1 cut(s) 2074
BfuAI ACCTGC 1 cut(s) 1693
BfuI GTATCC 2 cut(s) 609, 1917
BisI GCNGC 6 cut(s) 672, 1561, 2094, 2161, 2619, 2622
BlsI GCNGC 6 cut(s) 673, 1562, 2095, 2162, 2620, 2623
BmcAI AGTACT 2 cut(s) 197, 888
Bme18I GGWCC 1 cut(s) 1040
BmeRI GACNNNNNGTC 1 cut(s) 2497
BmeT110I CYCGRG 1 cut(s) 2285
BmgT120I GGNCC 2 cut(s) 1040, 2033
BmiI GGNNCC 8 cut(s) 272, 508, 679, 1041, 1559, 2283, 2638, 2686
BmrI ACTGGG 2 cut(s) 1172, 1269
BmsI GCATC 7 cut(s) 370, 387, 1705, 2080, 2246, 2584, 2605
BmuI ACTGGG 2 cut(s) 1172, 1269
BpiI GAAGAC 2 cut(s) 354, 1481
BplI GAGNNNNNCTC 8 cut(s) 146, 178, 1190, 1222, 1702, 1734, 2720, 2752
Bpu10I CCTNAGC 1 cut(s) 942
BpuEI CTTGAG 6 cut(s) 506, 1080, 2000, 2096, 2372, 2564
BpuMI CCSGG 1 cut(s) 2740
Bsa29I ATCGAT 1 cut(s) 2724
BsaHI GRCGYC 1 cut(s) 158
BsaI GGTCTC 2 cut(s) 135, 176
BsaJI CCNNGG 5 cut(s) 65, 968, 1035, 1879, 2688
BsaWI WCCGGW 2 cut(s) 786, 1469
Bsc4I CCNNNNNNNGG 3 cut(s) 815, 1691, 2476
Bse118I RCCGGY 2 cut(s) 786, 2120
Bse1I ACTGG 8 cut(s) 466, 590, 1178, 1264, 1336, 1357, 1525, 2497
Bse3DI GCAATG 3 cut(s) 666, 1062, 2426
BseCI ATCGAT 1 cut(s) 2724
BseDI CCNNGG 5 cut(s) 65, 968, 1035, 1879, 2688
BseLI CCNNNNNNNGG 3 cut(s) 815, 1691, 2476
BseMI GCAATG 3 cut(s) 666, 1062, 2426
BseMII CTCAG 8 cut(s) 157, 513, 559, 922, 956, 1221, 2616, 2655
BseNI ACTGG 8 cut(s) 466, 590, 1178, 1264, 1336, 1357, 1525, 2497
BseRI GAGGAG 2 cut(s) 20, 1402
BseSI GKGCMC 1 cut(s) 2267
BseXI GCAGC 5 cut(s) 658, 2080, 2172, 2605, 2633
BseYI CCCAGC 1 cut(s) 2301
BsgI GTGCAG 2 cut(s) 594, 1716
Bsh1236I CGCG 1 cut(s) 402
Bsh1285I CGRYCG 1 cut(s) 2724
BshFI GGCC 4 cut(s) 730, 2034, 2560, 2743
BshNI GGYRCC 1 cut(s) 270
BshTI ACCGGT 1 cut(s) 786
BshVI ATCGAT 1 cut(s) 2724
BsiEI CGRYCG 1 cut(s) 2724
BsiHKCI CYCGRG 1 cut(s) 2285
BsiSI CCGG 5 cut(s) 218, 787, 1470, 2121, 2740
BslFI GGGAC 2 cut(s) 29, 1053
BslI CCNNNNNNNGG 3 cut(s) 815, 1691, 2476
BsmAI GTCTC 5 cut(s) 135, 149, 176, 556, 962
BsmBI CGTCTC 2 cut(s) 149, 962
BsmFI GGGAC 2 cut(s) 29, 1053
BsmI GAATGC 1 cut(s) 1057
BsnI GGCC 4 cut(s) 730, 2034, 2560, 2743
Bso31I GGTCTC 2 cut(s) 135, 176
BsoBI CYCGRG 1 cut(s) 2285
Bsp1286I GDGCHC 1 cut(s) 2267
Bsp143I GATC 9 cut(s) 112, 136, 1165, 1210, 1986, 2064, 2281, 2692, 2721
BspACI CCGC 7 cut(s) 221, 1043, 1161, 1404, 1537, 1554, 1561
BspANI GGCC 4 cut(s) 730, 2034, 2560, 2743
BspCNI CTCAG 8 cut(s) 156, 514, 558, 921, 955, 1220, 2615, 2654
BspDI ATCGAT 1 cut(s) 2724
BspFNI CGCG 1 cut(s) 402
BspLI GGNNCC 8 cut(s) 272, 508, 679, 1041, 1559, 2283, 2638, 2686
BspMAI CTGCAG 1 cut(s) 2623
BspMI ACCTGC 1 cut(s) 1693
BspPI GGATC 6 cut(s) 1160, 1205, 2072, 2276, 2289, 2700
BspT107I GGYRCC 1 cut(s) 270
BspTNI GGTCTC 2 cut(s) 135, 176
BsrBI CCGCTC 1 cut(s) 1045
BsrDI GCAATG 3 cut(s) 666, 1062, 2426
BsrFI RCCGGY 2 cut(s) 786, 2120
BsrI ACTGG 8 cut(s) 466, 590, 1178, 1264, 1336, 1357, 1525, 2497
BssAI RCCGGY 2 cut(s) 786, 2120
BssECI CCNNGG 5 cut(s) 65, 968, 1035, 1879, 2688
BssMI GATC 9 cut(s) 112, 136, 1165, 1210, 1986, 2064, 2281, 2692, 2721
BssNI GRCGYC 1 cut(s) 158
Bst4CI ACNGT 8 cut(s) 433, 537, 1295, 1446, 1477, 1823, 1947, 2269
Bst6I CTCTTC 3 cut(s) 1296, 1755, 1917
BstACI GRCGYC 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 404, 826
BstENI CCTNNNNNAGG 1 cut(s) 1689
BstFNI CGCG 1 cut(s) 402
BstH2I RGCGCY 1 cut(s) 2074
BstHHI GCGC 4 cut(s) 402, 1690, 2073, 2545
BstKTI GATC 9 cut(s) 115, 139, 1168, 1213, 1989, 2067, 2284, 2695, 2724
BstMAI GTCTC 5 cut(s) 135, 149, 176, 556, 962
BstMBI GATC 9 cut(s) 112, 136, 1165, 1210, 1986, 2064, 2281, 2692, 2721
BstMCI CGRYCG 1 cut(s) 2724
BstMWI GCNNNNNNNGC 4 cut(s) 1022, 1560, 2090, 2522
BstNSI RCATGY 1 cut(s) 828
BstSFI CTRYAG 4 cut(s) 165, 756, 2268, 2619
BstSLI GKGCMC 1 cut(s) 2267
BstUI CGCG 1 cut(s) 402
BstV1I GCAGC 5 cut(s) 658, 2080, 2172, 2605, 2633
BstV2I GAAGAC 2 cut(s) 354, 1481
BstX2I RGATCY 3 cut(s) 1210, 2281, 2692
BstXI CCANNNNNNTGG 3 cut(s) 391, 1185, 1970
BstYI RGATCY 3 cut(s) 1210, 2281, 2692
Bsu15I ATCGAT 1 cut(s) 2724
BsuI GTATCC 2 cut(s) 609, 1917
BsuRI GGCC 4 cut(s) 730, 2034, 2560, 2743
BsuTUI ATCGAT 1 cut(s) 2724
BtgZI GCGATG 1 cut(s) 434
BtsI GCAGTG 1 cut(s) 1735
BveI ACCTGC 1 cut(s) 1693
Cac8I GCNNGC 2 cut(s) 404, 826
CaiI CAGNNNCTG 1 cut(s) 638
CfoI GCGC 4 cut(s) 402, 1690, 2073, 2545
Cfr10I RCCGGY 2 cut(s) 786, 2120
Cfr13I GGNCC 2 cut(s) 1040, 2033
ClaI ATCGAT 1 cut(s) 2724
CsiI ACCWGGT 1 cut(s) 1938
Csp6I GTAC 7 cut(s) 196, 271, 514, 887, 1335, 1581, 1943
CspAI ACCGGT 1 cut(s) 786
CspCI CAANNNNNGTGG 2 cut(s) 2586, 2621
CviAII CATG 8 cut(s) 511, 825, 1170, 1185, 1664, 1999, 2185, 2243
CviQI GTAC 7 cut(s) 196, 271, 514, 887, 1335, 1581, 1943
DpnI GATC 9 cut(s) 114, 138, 1167, 1212, 1988, 2066, 2283, 2694, 2723
DpnII GATC 9 cut(s) 112, 136, 1165, 1210, 1986, 2064, 2281, 2692, 2721
DraI TTTAAA 1 cut(s) 1009
DriI GACNNNNNGTC 1 cut(s) 2497
EaeI YGGCCR 1 cut(s) 2741
Eam1104I CTCTTC 3 cut(s) 1296, 1755, 1917
Eam1105I GACNNNNNGTC 1 cut(s) 2497
EarI CTCTTC 3 cut(s) 1296, 1755, 1917
EciI GGCGGA 2 cut(s) 236, 1419
Eco31I GGTCTC 2 cut(s) 135, 176
Eco32I GATATC 1 cut(s) 2361
Eco47I GGWCC 1 cut(s) 1040
Eco57I CTGAAG 3 cut(s) 879, 1257, 2106
Eco88I CYCGRG 1 cut(s) 2285
EcoNI CCTNNNNNAGG 1 cut(s) 1689
EcoRI GAATTC 1 cut(s) 2656
EcoRV GATATC 1 cut(s) 2361
EcoT22I ATGCAT 1 cut(s) 2004
Esp3I CGTCTC 2 cut(s) 149, 962
FaeI CATG 8 cut(s) 514, 828, 1173, 1188, 1667, 2002, 2188, 2246
FaqI GGGAC 2 cut(s) 29, 1053
FatI CATG 8 cut(s) 510, 824, 1169, 1184, 1663, 1998, 2184, 2242
FauI CCCGC 1 cut(s) 1547
FbaI TGATCA 1 cut(s) 136
FblI GTMKAC 1 cut(s) 663
Fnu4HI GCNGC 6 cut(s) 672, 1561, 2094, 2161, 2619, 2622
Fsp4HI GCNGC 6 cut(s) 672, 1561, 2094, 2161, 2619, 2622
FspBI CTAG 6 cut(s) 267, 870, 884, 1116, 1245, 2540
FspI TGCGCA 1 cut(s) 1689
GlaI GCGC 4 cut(s) 401, 1689, 2072, 2544
GluI GCNGC 6 cut(s) 672, 1561, 2094, 2161, 2619, 2622
GsaI CCCAGC 1 cut(s) 2305
HaeII RGCGCY 1 cut(s) 2074
HaeIII GGCC 4 cut(s) 730, 2034, 2560, 2743
HapII CCGG 5 cut(s) 218, 787, 1470, 2121, 2740
HhaI GCGC 4 cut(s) 402, 1690, 2073, 2545
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 8 cut(s) 514, 828, 1173, 1188, 1667, 2002, 2188, 2246
Hin6I GCGC 4 cut(s) 400, 1688, 2071, 2543
HinP1I GCGC 4 cut(s) 400, 1688, 2071, 2543
HincII GTYRAC 7 cut(s) 664, 693, 975, 1398, 1467, 2310, 2401
HindII GTYRAC 7 cut(s) 664, 693, 975, 1398, 1467, 2310, 2401
HpaI GTTAAC 3 cut(s) 975, 1467, 2401
HpaII CCGG 5 cut(s) 218, 787, 1470, 2121, 2740
HphI GGTGA 3 cut(s) 104, 164, 1175
Hpy166II GTNNAC 9 cut(s) 664, 693, 975, 1363, 1398, 1467, 1816, 2310, 2401
Hpy188I TCNGA 9 cut(s) 379, 523, 634, 1210, 1276, 1753, 2203, 2572, 2644
Hpy8I GTNNAC 9 cut(s) 664, 693, 975, 1363, 1398, 1467, 1816, 2310, 2401
Hpy99I CGWCG 3 cut(s) 44, 205, 665
HpyCH4III ACNGT 8 cut(s) 433, 537, 1295, 1446, 1477, 1823, 1947, 2269
HpyCH4IV ACGT 4 cut(s) 158, 203, 318, 2325
HpyF10VI GCNNNNNNNGC 4 cut(s) 1022, 1560, 2090, 2522
HpySE526I ACGT 4 cut(s) 158, 203, 318, 2325
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 8 cut(s) 514, 828, 1173, 1188, 1667, 2002, 2188, 2246
HspAI GCGC 4 cut(s) 400, 1688, 2071, 2543
KpnI GGTACC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 136
KspAI GTTAAC 3 cut(s) 975, 1467, 2401
Kzo9I GATC 9 cut(s) 112, 136, 1165, 1210, 1986, 2064, 2281, 2692, 2721
LmnI GCTCC 4 cut(s) 1103, 1557, 1723, 2234
Lsp1109I GCAGC 5 cut(s) 658, 2080, 2172, 2605, 2633
LweI GCATC 7 cut(s) 370, 387, 1705, 2080, 2246, 2584, 2605
MabI ACCWGGT 1 cut(s) 1938
MaeI CTAG 6 cut(s) 267, 870, 884, 1116, 1245, 2540
MaeII ACGT 4 cut(s) 158, 203, 318, 2325
MaeIII GTNAC 6 cut(s) 239, 253, 299, 1202, 1906, 2321
MalI GATC 9 cut(s) 114, 138, 1167, 1212, 1988, 2066, 2283, 2694, 2723
MbiI CCGCTC 1 cut(s) 1045
MboI GATC 9 cut(s) 112, 136, 1165, 1210, 1986, 2064, 2281, 2692, 2721
MfeI CAATTG 2 cut(s) 705, 2059
MflI RGATCY 3 cut(s) 1210, 2281, 2692
MhlI GDGCHC 1 cut(s) 2267
MlyI GAGTC 5 cut(s) 433, 629, 875, 1401, 2738
Mph1103I ATGCAT 1 cut(s) 2004
MroXI GAANNNNTTC 2 cut(s) 1699, 2112
MslI CAYNNNNRTG 1 cut(s) 2028
MspA1I CMGCKG 1 cut(s) 755
MspI CCGG 5 cut(s) 218, 787, 1470, 2121, 2740
MunI CAATTG 2 cut(s) 705, 2059
Mva1269I GAATGC 1 cut(s) 1057
MvnI CGCG 1 cut(s) 402
MwoI GCNNNNNNNGC 4 cut(s) 1022, 1560, 2090, 2522
NciI CCSGG 1 cut(s) 2740
NdeII GATC 9 cut(s) 112, 136, 1165, 1210, 1986, 2064, 2281, 2692, 2721
NlaIII CATG 8 cut(s) 514, 828, 1173, 1188, 1667, 2002, 2188, 2246
NlaIV GGNNCC 8 cut(s) 272, 508, 679, 1041, 1559, 2283, 2638, 2686
NmuCI GTSAC 2 cut(s) 253, 2321
NsbI TGCGCA 1 cut(s) 1689
NsiI ATGCAT 1 cut(s) 2004
NspI RCATGY 1 cut(s) 828
PaeI GCATGC 1 cut(s) 828
PctI GAATGC 1 cut(s) 1057
PdmI GAANNNNTTC 2 cut(s) 1699, 2112
PfeI GAWTC 5 cut(s) 224, 1028, 1678, 1727, 2103
PflFI GACNNNGTC 1 cut(s) 638
PfoI TCCNGGA 1 cut(s) 892
PinAI ACCGGT 1 cut(s) 786
PkrI GCNGC 6 cut(s) 673, 1562, 2095, 2162, 2620, 2623
Ple19I CGATCG 1 cut(s) 2724
PleI GAGTC 5 cut(s) 432, 629, 874, 1401, 2737
PpsI GAGTC 5 cut(s) 432, 629, 874, 1401, 2737
PshBI ATTAAT 2 cut(s) 564, 714
Psp1406I AACGTT 1 cut(s) 318
PspFI CCCAGC 1 cut(s) 2301
PspN4I GGNNCC 8 cut(s) 272, 508, 679, 1041, 1559, 2283, 2638, 2686
PspPI GGNCC 2 cut(s) 1040, 2033
PstI CTGCAG 1 cut(s) 2623
PstNI CAGNNNCTG 1 cut(s) 638
PsuI RGATCY 3 cut(s) 1210, 2281, 2692
PsyI GACNNNGTC 1 cut(s) 638
PvuI CGATCG 1 cut(s) 2724
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 7 cut(s) 197, 272, 515, 888, 1336, 1582, 1944
RsaNI GTAC 7 cut(s) 196, 271, 514, 887, 1335, 1581, 1943
RseI CAYNNNNRTG 1 cut(s) 2028
SalI GTCGAC 1 cut(s) 662
SatI GCNGC 6 cut(s) 672, 1561, 2094, 2161, 2619, 2622
Sau3AI GATC 9 cut(s) 112, 136, 1165, 1210, 1986, 2064, 2281, 2692, 2721
Sau96I GGNCC 2 cut(s) 1040, 2033
ScaI AGTACT 2 cut(s) 197, 888
SchI GAGTC 5 cut(s) 433, 629, 875, 1401, 2738
SduI GDGCHC 1 cut(s) 2267
SexAI ACCWGGT 1 cut(s) 1938
SfaNI GCATC 7 cut(s) 370, 387, 1705, 2080, 2246, 2584, 2605
SfcI CTRYAG 4 cut(s) 165, 756, 2268, 2619
SinI GGWCC 1 cut(s) 1040
SmiI ATTTAAAT 1 cut(s) 1009
SmiMI CAYNNNNRTG 1 cut(s) 2028
SmlI CTYRAG 6 cut(s) 485, 1095, 2015, 2075, 2351, 2579
SmoI CTYRAG 6 cut(s) 485, 1095, 2015, 2075, 2351, 2579
SphI GCATGC 1 cut(s) 828
SsiI CCGC 7 cut(s) 221, 1043, 1161, 1404, 1537, 1554, 1561
SspI AATATT 3 cut(s) 236, 928, 1255
SspMI CTAG 6 cut(s) 267, 870, 884, 1116, 1245, 2540
SwaI ATTTAAAT 1 cut(s) 1009
TaaI ACNGT 8 cut(s) 433, 537, 1295, 1446, 1477, 1823, 1947, 2269
TaiI ACGT 4 cut(s) 161, 206, 321, 2328
TatI WGTACW 3 cut(s) 195, 513, 886
TauI GCSGC 1 cut(s) 1563
TfiI GAWTC 5 cut(s) 224, 1028, 1678, 1727, 2103
TseFI GTSAC 2 cut(s) 253, 2321
TseI GCWGC 5 cut(s) 671, 2093, 2160, 2618, 2621
Tsp45I GTSAC 2 cut(s) 253, 2321
TspDTI ATGAA 9 cut(s) 597, 978, 1367, 1526, 1844, 1851, 1987, 2132, 2400
TspGWI ACGGA 1 cut(s) 2562
Tth111I GACNNNGTC 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 1040
VspI ATTAAT 2 cut(s) 564, 714
XagI CCTNNNNNAGG 1 cut(s) 1689
XapI RAATTY 5 cut(s) 99, 329, 1010, 1892, 2656
XbaI TCTAGA 1 cut(s) 869
XceI RCATGY 1 cut(s) 828
XmiI GTMKAC 1 cut(s) 663
XmnI GAANNNNTTC 2 cut(s) 1699, 2112
XspI CTAG 6 cut(s) 267, 870, 884, 1116, 1245, 2540
ZraI GACGTC 1 cut(s) 159
ZrmI AGTACT 2 cut(s) 197, 888
Zsp2I ATGCAT 1 cut(s) 2004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.