MD04G1109900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0021183
Physical Location & Seq
Forward (+)
19460462 .. 19461058
597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1109900.v1.1.491

Sequence Viewer

Length: 597 bp
ATGTTGATTCTACTCCTCCTAGCCTTCTTGGCAGCAATCACTCCCGCCACCGAAGCCGTCTGCTCAGAGGCCATGCTCACTTCCCTCTCGTCTTCCGGCTTCACACTCTTCGCCCATGCTCTCCATTCCCAAAACCTCACAGCCACCGCCGCCGCCAGAGTCACTTGCTTTGTGCCACCTGACGACTGCTTCTTAGGCCAGGTCCTCAGCCCCACCACGCTTCGCGCTCACGTCTACACCTCCGGGGCCCTGGATTACAAAGCCCTCCTCAAGCTCCCACTCAACACCACCCTCACTGCTCACAACACACAGCTTGCTTTCGGTACCGATGGCAGAAGGGTCTCGTTCAACGACGTTCTTGTCACTGCCCCGAATCTCCACGTTGACGATTCTTGTGTTGTTCATGGCGTTGAAGGGCCGCTGGTGCCAATGCCGCCATTGACAGATGATGTTCCGAGGCCGAGAACCAAGAATGTGGCGTCTCCAGCATTGCCAAAGCACCGAGGCCGGCGTTTTGCGCAGATTGTCCGGTTCATTCGCCGTGTGCACCTCCCGATTTTTGTTGATGGGGATAAAAACACCACCAGCCTCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

21.34

Weight (kDa)

8.82

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021183)

Species Orthologous Gene IDs
malus_domestica MD04G1109900.v1.1
pyrus_communis pycom04g10610 pycom12g12730
rosa_multiflora Rmu_sc0006706.1_g000007
rosa_roxburghii Rroxscaffold_6G00413190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
Acc16I TGCGCA 1 cut(s) 519
Acc65I GGTACC 1 cut(s) 323
AccB1I GGYRCC 2 cut(s) 323, 424
AccI GTMKAC 1 cut(s) 234
AccII CGCG 1 cut(s) 225
AciI CCGC 6 cut(s) 45, 147, 150, 153, 419, 434
AcyI GRCGYC 1 cut(s) 479
AfaI GTAC 1 cut(s) 325
AgsI TTSAA 2 cut(s) 349, 413
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 2 cut(s) 198, 249
AluBI AGCT 2 cut(s) 274, 313
AluI AGCT 2 cut(s) 274, 313
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 2 cut(s) 346, 486
Alw44I GTGCAC 1 cut(s) 545
AoxI GGCC 6 cut(s) 69, 196, 246, 416, 458, 505
ApaI GGGCCC 1 cut(s) 250
ApaLI GTGCAC 1 cut(s) 545
ApeKI GCWGC 1 cut(s) 32
Asp718I GGTACC 1 cut(s) 323
AspLEI GCGC 2 cut(s) 227, 520
AspS9I GGNCC 4 cut(s) 202, 246, 247, 416
AsuC2I CCSGG 1 cut(s) 244
AvaII GGWCC 1 cut(s) 202
BaeGI GKGCMC 2 cut(s) 250, 549
BanI GGYRCC 2 cut(s) 323, 424
BanII GRGCYC 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 549
BbvCI CCTCAGC 1 cut(s) 206
BbvI GCAGC 1 cut(s) 44
BccI CCATC 2 cut(s) 323, 560
BceAI ACGGC 2 cut(s) 41, 525
BciT130I CCWGG 2 cut(s) 200, 251
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 2 cut(s) 346, 486
BfaI CTAG 1 cut(s) 20
BglI GCCNNNNNGGC 1 cut(s) 29
BisI GCNGC 5 cut(s) 33, 150, 153, 419, 434
BlsI GCNGC 5 cut(s) 34, 151, 154, 420, 435
Bme1390I CCNGG 3 cut(s) 200, 244, 251
Bme18I GGWCC 1 cut(s) 202
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 4 cut(s) 202, 246, 247, 416
BmiI GGNNCC 4 cut(s) 247, 248, 325, 426
BmrFI CCNGG 3 cut(s) 200, 244, 251
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 468
Bpu10I CCTNAGC 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 244
BsaHI GRCGYC 1 cut(s) 479
BsaI GGTCTC 1 cut(s) 346
BsaJI CCNNGG 4 cut(s) 243, 249, 455, 502
BsaWI WCCGGW 1 cut(s) 528
Bse118I RCCGGY 1 cut(s) 507
Bse3DI GCAATG 1 cut(s) 488
BseBI CCWGG 2 cut(s) 200, 251
BseDI CCNNGG 4 cut(s) 243, 249, 455, 502
BseMI GCAATG 1 cut(s) 488
BseMII CTCAG 2 cut(s) 78, 220
BseRI GAGGAG 2 cut(s) 5, 257
BseSI GKGCMC 2 cut(s) 250, 549
BseXI GCAGC 1 cut(s) 44
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 6 cut(s) 71, 198, 248, 418, 460, 507
BshNI GGYRCC 2 cut(s) 323, 424
BsiHKAI GWGCWC 1 cut(s) 549
BsiSI CCGG 4 cut(s) 96, 243, 508, 529
BsmAI GTCTC 2 cut(s) 346, 486
BsmBI CGTCTC 1 cut(s) 486
BsnI GGCC 6 cut(s) 71, 198, 248, 418, 460, 507
Bso31I GGTCTC 1 cut(s) 346
Bsp120I GGGCCC 1 cut(s) 246
Bsp1286I GDGCHC 2 cut(s) 250, 549
BspACI CCGC 6 cut(s) 45, 147, 150, 153, 419, 434
BspANI GGCC 6 cut(s) 71, 198, 248, 418, 460, 507
BspCNI CTCAG 2 cut(s) 77, 219
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 4 cut(s) 247, 248, 325, 426
BspT107I GGYRCC 2 cut(s) 323, 424
BspTNI GGTCTC 1 cut(s) 346
BsrDI GCAATG 1 cut(s) 488
BsrFI RCCGGY 1 cut(s) 507
BssAI RCCGGY 1 cut(s) 507
BssECI CCNNGG 4 cut(s) 243, 249, 455, 502
BssNI GRCGYC 1 cut(s) 479
Bst2UI CCWGG 2 cut(s) 200, 251
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 1 cut(s) 479
BstC8I GCNNGC 2 cut(s) 315, 509
BstDEI CTNAG 3 cut(s) 64, 193, 206
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 2 cut(s) 227, 520
BstMAI GTCTC 2 cut(s) 346, 486
BstMWI GCNNNNNNNGC 8 cut(s) 29, 53, 149, 195, 424, 433, 485, 517
BstNI CCWGG 2 cut(s) 200, 251
BstSCI CCNGG 3 cut(s) 198, 242, 249
BstSLI GKGCMC 2 cut(s) 250, 549
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 44
BstV2I GAAGAC 1 cut(s) 84
BstXI CCANNNNNNTGG 1 cut(s) 475
BsuRI GGCC 6 cut(s) 71, 198, 248, 418, 460, 507
BtrI CACGTC 1 cut(s) 232
BtsI GCAGTG 2 cut(s) 294, 363
BtsIMutI CAGTG 2 cut(s) 294, 363
Cac8I GCNNGC 2 cut(s) 315, 509
CfoI GCGC 2 cut(s) 227, 520
Cfr10I RCCGGY 1 cut(s) 507
Cfr13I GGNCC 4 cut(s) 202, 246, 247, 416
CseI GACGC 1 cut(s) 468
Csp6I GTAC 1 cut(s) 324
CviAII CATG 3 cut(s) 73, 116, 404
CviQI GTAC 1 cut(s) 324
DdeI CTNAG 3 cut(s) 64, 193, 206
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 250
Eco31I GGTCTC 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 202
EcoO109I RGGNCCY 3 cut(s) 202, 246, 247
EcoRII CCWGG 2 cut(s) 198, 249
EcoT38I GRGCYC 1 cut(s) 250
Esp3I CGTCTC 1 cut(s) 486
FaeI CATG 3 cut(s) 76, 119, 407
FaiI YATR 3 cut(s) 74, 117, 405
FatI CATG 3 cut(s) 72, 115, 403
FauI CCCGC 1 cut(s) 52
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 5 cut(s) 33, 150, 153, 419, 434
FriOI GRGCYC 1 cut(s) 250
Fsp4HI GCNGC 5 cut(s) 33, 150, 153, 419, 434
FspBI CTAG 1 cut(s) 20
FspI TGCGCA 1 cut(s) 519
GlaI GCGC 2 cut(s) 226, 519
GluI GCNGC 5 cut(s) 33, 150, 153, 419, 434
GsuI CTGGAG 1 cut(s) 468
HaeIII GGCC 6 cut(s) 71, 198, 248, 418, 460, 507
HapII CCGG 4 cut(s) 96, 243, 508, 529
HgaI GACGC 1 cut(s) 468
HhaI GCGC 2 cut(s) 227, 520
Hin1I GRCGYC 1 cut(s) 479
Hin1II CATG 3 cut(s) 76, 119, 407
Hin6I GCGC 2 cut(s) 225, 518
HinP1I GCGC 2 cut(s) 225, 518
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 4 cut(s) 7, 159, 373, 389
HpaII CCGG 4 cut(s) 96, 243, 508, 529
Hpy166II GTNNAC 3 cut(s) 235, 385, 547
Hpy188I TCNGA 2 cut(s) 67, 456
Hpy188III TCNNGA 1 cut(s) 553
Hpy8I GTNNAC 3 cut(s) 235, 385, 547
Hpy99I CGWCG 1 cut(s) 356
HpyAV CCTTC 3 cut(s) 34, 330, 407
HpyCH4IV ACGT 3 cut(s) 231, 354, 381
HpyCH4V TGCA 1 cut(s) 547
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 53, 149, 195, 424, 433, 485, 517
HpyF3I CTNAG 3 cut(s) 64, 193, 206
HpySE526I ACGT 3 cut(s) 231, 354, 381
Hsp92I GRCGYC 1 cut(s) 479
Hsp92II CATG 3 cut(s) 76, 119, 407
HspAI GCGC 2 cut(s) 225, 518
KpnI GGTACC 1 cut(s) 327
KroI GCCGGC 1 cut(s) 507
KroNI GCCGGC 1 cut(s) 509
LmnI GCTCC 1 cut(s) 279
Lsp1109I GCAGC 1 cut(s) 44
MaeI CTAG 1 cut(s) 20
MaeII ACGT 3 cut(s) 231, 354, 381
MaeIII GTNAC 2 cut(s) 160, 361
MboII GAAGA 2 cut(s) 84, 100
MhlI GDGCHC 2 cut(s) 250, 549
MlyI GAGTC 1 cut(s) 168
MroNI GCCGGC 1 cut(s) 507
MspA1I CMGCKG 1 cut(s) 421
MspI CCGG 4 cut(s) 96, 243, 508, 529
MspR9I CCNGG 3 cut(s) 200, 244, 251
MvaI CCWGG 2 cut(s) 200, 251
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 8 cut(s) 29, 53, 149, 195, 424, 433, 485, 517
NaeI GCCGGC 1 cut(s) 509
NciI CCSGG 1 cut(s) 244
NgoMIV GCCGGC 1 cut(s) 507
NlaIII CATG 3 cut(s) 76, 119, 407
NlaIV GGNNCC 4 cut(s) 247, 248, 325, 426
NmeAIII GCCGAG 1 cut(s) 486
NmuCI GTSAC 2 cut(s) 160, 361
NsbI TGCGCA 1 cut(s) 519
PdiI GCCGGC 1 cut(s) 509
PfeI GAWTC 3 cut(s) 7, 373, 389
PkrI GCNGC 5 cut(s) 34, 151, 154, 420, 435
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
PpuMI RGGWCCY 1 cut(s) 202
Psp5II RGGWCCY 1 cut(s) 202
Psp6I CCWGG 2 cut(s) 198, 249
PspGI CCWGG 2 cut(s) 198, 249
PspN4I GGNNCC 4 cut(s) 247, 248, 325, 426
PspOMI GGGCCC 1 cut(s) 246
PspPI GGNCC 4 cut(s) 202, 246, 247, 416
PspPPI RGGWCCY 1 cut(s) 202
RsaI GTAC 1 cut(s) 325
RsaNI GTAC 1 cut(s) 324
SatI GCNGC 5 cut(s) 33, 150, 153, 419, 434
Sau96I GGNCC 4 cut(s) 202, 246, 247, 416
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 3 cut(s) 200, 244, 251
SduI GDGCHC 2 cut(s) 250, 549
SinI GGWCC 1 cut(s) 202
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SsiI CCGC 6 cut(s) 45, 147, 150, 153, 419, 434
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 3 cut(s) 198, 242, 249
TaiI ACGT 3 cut(s) 234, 357, 384
TauI GCSGC 4 cut(s) 152, 155, 421, 436
TfiI GAWTC 3 cut(s) 7, 373, 389
TscAI CASTG 2 cut(s) 301, 370
TseFI GTSAC 2 cut(s) 160, 361
TseI GCWGC 1 cut(s) 32
Tsp45I GTSAC 2 cut(s) 160, 361
TspDTI ATGAA 2 cut(s) 392, 523
TspRI CASTG 2 cut(s) 301, 370
VneI GTGCAC 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 234
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.