MD04G1133500.v1.1

Legume lectin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0020828
Physical Location & Seq
Reverse (-)
22007812 .. 22013498
5687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1133500.v1.1.491

Sequence Viewer

Length: 3876 bp
ATGTTGTTCAAGTTTGTAATACTCATTCTGACAAGCGTAGCAGCAGCTGAAGATTTCAATTTCACCTACCTTGGCTTCCGATCTGCAAATCTTAGCCTAGAAGGCGTAGCAGAAGTGACATCCAATGGTATTCTGAGGGTTACAAATGATACTGTTCAGAGCATTGGTCATGCCTTCTATCCCAACCCAGTAACTTTCAAGAACTCATCTGATGGACCAGTTTTATCTTTCTCTTCCACATTCATCTTTGCTATGAGATATAAAGTTCGCCAAGGCCATGGAATCGCCTTTGTCATTGCTCCAACAAAAGGGCTGCCTGGAGCTTCTCCAGTTGGCTTCTTTGGCCTTTTCAACAGGACGAATGATGGCAGTCCAACGAATCATGTTTTTGCTGTAGAGCTGGACACTTTCGAGAACCCAGAATTCAGTGACATCAATGACAACCATGTCGGGATTGATATTAATGGTGTAGTCTCTGTGGTATCTGCCCCAGCCGGATATTATGCTGATGAAAGATTGATCACGAGTGGGCAGCCTGTGCAAGTTTGGGTGGACTATGATGGTACCAAGAAGCAAATCAATGTCTCTATGGCACTGGTTAGTAATGGTAAGCCCTATAATCCACTTTTGTCTTTGACTCGTGACCTTTCCCCAGTCCTTCACAGAACCATGTACATGGGTTTCTCCGCATCCACTGGTAATCTAACAGCAATTAATTATGTTTTGGGCTGGAGCTTTAAGATGAACGGTCATGCTGAAGAACTTGGTCTCTCACAACTTCCTAAGATGCCTCGTTTAGAACCTAAAAAAATACCTAAACTTTTGACCATTGGCCTGCCTGTGATATTAGTGAGTTCCGCTTTGCTAGCATTTGTTTTTTGGGTATACTATGCCATAAGAAGAAAGAAGAAGTTTGCAGAACTGCTTGAAGACTGGGAGCTTGAGTATGGGCCTCAGAGGTTTAAATATAGAGAGTTATATATTGCCACAAAAGGGTTTCGGGAGAAGGAGCTTTTGGGCAAGGGTGGATTTGGTAAGGTTTACAGAGGTATATCGCCGACCTCGGGAACTGTGATTGCTGTGAAGAGGGTCTCGCACGAATCAAGGCAGGGGATGAAGGAGTTTGTGGCAGAAATTGTCAGCAATGGACGTCTTCGTCACCGGAATTTAGTTCCTTTGTTGGGCTATTGCAGAAGAAAAGGGGAGCTATTGTTGGTCTATGAGTACATGCCTAATGGAAGTCTGGACAAGTACCTCTTTGACCGACCGGCAGTCACTCTCGATTGGAGCCAGAGATTTATAGTCATAAGAGGTGTAGCATTAGGTCTGCTTTATCTGCACGAAGAATGGGATCAGGTTGTGGTTCACAGAGATGTCAAGGCCAGTAATATTTTACTAGATGGGGAATGGAATGGAAGGTTAGGAGATTTTGGGCTTGCAAGATTATATGACCACGGCGCAGACCCTCAAACTACTCATATAGCTGGAACATTTGGGTATCTAGCTCCAGAGCATACAAGATCATGTCGGGCGACAACGAGTAGTGATGTGTTTGCTTTCGGTGTATGTTTGCTGGAAGTTGCCTGTGGAAGAAGGCCAATAGAATATCATGGCTCCGAGAATGTGATTTTGGTTGATTGGGTGTTTTCTTGTTGGGAAGGACGTAATATTCTTGAGGCGAAAGATCCAAAATTAGGTAATGATTTTGTAGCAGAGGAGGTGGAGTTGGTGTTGAAGCTTGGGCTGTTTTGCTGCCAATCAGAGCCTTCAATGAGGCCAAGGATGCGCCAAGTTGTGCAGCATTTGGAGGATAAAGTTCCTTTGCCTGCAGAGTTTTCACTTCTTGGTCTCTCCTCACGTGGCTTAGGCATTTCTCATAATGAAGGTTTTGATGAATATACAATGGCCCCAGGGTCTTCAGTGTCATCGTATGTTCCAGATTCGTCATTCCTCTCCTGCGTTGCTGAAGATCTCACTTTCACGTACCATGGTTTCCGGTCTGCGAATCTTAGCCTAGATGGCATAGCAGAAGTGACATTCAATGGCCTTCTGAAGCTTACAAATGAGACCGCCTTCAAGATGGGTCATGCCTTCTATCCCAACCCAGTAACTTTCAAGAACTCATCTTGTGGCACAGTTTTCTCCTTCTCTGTCACCTTCGTCTTTGCTATGAGATATCCTGATCTCTGTGATCATGGAATAGCCTTTATCATTGCTCCAACCAGAGGGCTGCCCGGAGCTTCTTCAAGCCAGTACCTAGGCCTTTTCAACAGGACAAACAATGGCAGTCCTACCAATCATGTTTTTGCTGTAGAGCTTGACACTTTCAAGAACGAAGAGTTCAAAGACATCAATGACAACCATGTAGGGATTGATATTAATGGTATGGTTTCTGAGGTGTCTGCTCCAGCAGGATATTATGTTGGAGAAAGAGTGATTACGAATGGCCAACCCGTGCAAGTATGGGTGGACTATGATGGTAGCAAGAAGCAAATAAATGTCTCTATGTCTCCAGTTAGTAATGGTAAGCCCTACATTCCACTTTTGTCTTTGACGCGTGACCTTTCCCCAATCCTTAACGAAATCATGTACATAGGCTTCTCCGCGTCCACTAGCAAGCTTACTGCATCTGCATATGTTTTGGGGTGGAGCTTTAAGTTGAATGGCCAGGCTGAAGAGATTGATCTCTCAAAGCTTCCCAAGCTGCCTCCGCTAGGACCAAAAAAAAGATCTAAACATTTGACCATTGCTCTGCCTGTAATAATTGGGAGTTCGGTTTTGTTAGCACTTCTTTATGGTGTACTCTTTGCCATAAGAAGAAACAAGTTTGCGGAACTGCTTGAAGACTGGGAGCTTGAGTACGGACCTCAAAGGTTTAAATACAAAGAGTTATATATTGCCACAAAAGGGTTTAGGGAGAAGGAGCTTTTGGGGAAGGGTGGATTTGGTAAGGTATATAAAGGCATATTACCCAACACAAAAGCTGAGATTGCTGTGAAGAGGGAGTCACACGAGTCAAGGCAGGGGATGAAGGAGTTCGTGGCAGAAATTGTTAGCAATGGAAGACTTCGTCACCGAAACCTAGTTCCTTTGTTGGGCTATTGCAGAAGAAAAGGAGAGCTGCTGTTGGTGTATGAGTACATGCCTAATGGAAGCCTCGACAAGTACCTCTATGGCCAACCGACGGTCACTCTCAATTGGAGCCAGAGATTTAGGGTCATCAAAGGCGTAGCATCAGGGTTGCTTTATCTCCATGAAGAATGGGATCAGGTTGTGATTCACAGAGATGTGAAGGCCAGTAATGTATTACTCGATGGAGAATGGAACGGAAAGCTAGGAGATTTTGGGCTTGCAAGACTACATGACCACGGCGCAGACCCTCAAACTACTCATATAGCTGGAACATTTGGGTACCTAGCTCCTGAGCACACAAGATCAGGCCGGGCCACAACGAGCACTGATGTGTTCTCTTTTGGGGTATTTTTGCTTGAAGTTGCCTGTGGAAGGAAGCCAATCGAGACGCATGATTCAGAGGTTGTCATTTTAGTTGACCGGGTGTTTTGTTGTTGGAAAAGAAGTAACATTCTTGAGGCAAAAGATCCAAACTTGGGAACCGAATTTGTAGCAGAGGAGGTGGAGTTGGTATTGAAGCTCGGGCTGTTATGCTCTCAGTCAGAGCCTGCAGCTAGGCCAAGCATGCGCCAAGTTGTGCAGTACTTGGAGGGAGACCTTCTTTTGCCGGAGCTGTCACCCCTTGGTGTTTCTGCCAGCGGCTTATCGTTTCCTCACAAAGAAGGTTTTGACGAATTTGCAACGGGCACGGGGTTGTCGCATTCATATGCTTCAGAGTCATCACGCCTCTCAGGTGGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase

Protein Analysis

1292

Amino Acids

143.27

Weight (kDa)

6.82

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 20 - 261 5.6e-78 Legume lectin domain
Lectin_L-type_dom PF18483 30 - 239 2.9e-09 Legume lectin beta-barrel domain
Pkinase PF00069 333 - 601 3.2e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 336 - 602 4.8e-41 Protein tyrosine and serine/threonine kinase
Lectin_legB PF00139 658 - 900 7.6e-79 Legume lectin domain
Lectin_L-type_dom PF18483 680 - 877 6.3e-07 Legume lectin beta-barrel domain
Pkinase PF00069 971 - 1238 3.7e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 974 - 1240 8.7e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020828)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37710
malus_domestica MD04G1133500.v1.1 MD12G1146300.v1.1 MD12G1146500.v1.1
pyrus_communis pycom12g14020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 446, 1155
AatII GACGTC 1 cut(s) 1153
Acc65I GGTACC 2 cut(s) 563, 3411
AccB1I GGYRCC 2 cut(s) 563, 3411
AccI GTMKAC 1 cut(s) 885
AccII CGCG 2 cut(s) 2557, 2606
AciI CCGC 8 cut(s) 687, 858, 2070, 2604, 2711, 2831, 3771, 3871
AclWI GGATC 4 cut(s) 1359, 1679, 3273, 3593
AcoI YGGCCR 4 cut(s) 2446, 2665, 3175, 3868
AcsI RAATTY 4 cut(s) 422, 1165, 3617, 3806
AcuI CTGAAG 7 cut(s) 69, 777, 1902, 1986, 2072, 2694, 3828
AcvI CACGTG 1 cut(s) 1859
AcyI GRCGYC 1 cut(s) 1150
AflIII ACRYGT 1 cut(s) 2555
AhdI GACNNNNNGTC 1 cut(s) 1271
AjnI CCWGG 3 cut(s) 316, 1909, 2667
AjuI GAANNNNNNNTTGG 6 cut(s) 998, 1030, 1613, 1645, 2912, 2944
AleI CACNNNNGTG 1 cut(s) 3461
Alw21I GWGCWC 2 cut(s) 3429, 3458
AlwI GGATC 4 cut(s) 1359, 1679, 3273, 3593
AlwNI CAGNNNCTG 2 cut(s) 47, 3680
Ama87I CYCGRG 2 cut(s) 1063, 3653
ApoI RAATTY 4 cut(s) 422, 1165, 3617, 3806
AseI ATTAAT 3 cut(s) 462, 714, 2379
Asp700I GAANNNNTTC 1 cut(s) 3035
Asp718I GGTACC 2 cut(s) 563, 3411
AspA2I CCTAGG 1 cut(s) 2257
AspLEI GCGC 4 cut(s) 1460, 1788, 3374, 3702
AspS9I GGNCC 6 cut(s) 215, 950, 1906, 2717, 2864, 3444
AsuC2I CCSGG 3 cut(s) 2235, 3443, 3554
AsuHPI GGTGA 5 cut(s) 55, 1151, 2146, 3065, 3741
AsuNHI GCTAGC 1 cut(s) 865
AvaI CYCGRG 2 cut(s) 1063, 3653
AvaII GGWCC 3 cut(s) 215, 2717, 2864
AvrII CCTAGG 1 cut(s) 2257
BaeGI GKGCMC 1 cut(s) 3821
BalI TGGCCA 3 cut(s) 2448, 2667, 3177
BanI GGYRCC 2 cut(s) 563, 3411
BarI GAAGNNNNNNTAC 2 cut(s) 2582, 2614
BauI CACGAG 3 cut(s) 523, 639, 3011
BbrPI CACGTG 1 cut(s) 1859
BbsI GAAGAC 5 cut(s) 936, 1145, 1908, 2850, 3070
Bbv12I GWGCWC 2 cut(s) 3429, 3458
BccI CCATC 8 cut(s) 206, 359, 554, 1394, 2012, 2074, 2471, 3308
BceAI ACGGC 2 cut(s) 1471, 3385
BciT130I CCWGG 3 cut(s) 318, 1911, 2669
BclI TGATCA 2 cut(s) 519, 2191
BcnI CCSGG 3 cut(s) 2235, 3443, 3554
BfmI CTRYAG 4 cut(s) 393, 1827, 2310, 3681
BglI GCCNNNNNGGC 2 cut(s) 102, 2019
BglII AGATCT 2 cut(s) 1969, 2729
BlnI CCTAGG 1 cut(s) 2257
BmcAI AGTACT 1 cut(s) 3716
Bme1390I CCNGG 6 cut(s) 318, 1911, 2235, 2669, 3443, 3554
Bme18I GGWCC 3 cut(s) 215, 2717, 2864
BmeRI GACNNNNNGTC 1 cut(s) 1271
BmeT110I CYCGRG 2 cut(s) 1063, 3653
BmgT120I GGNCC 6 cut(s) 215, 950, 1906, 2717, 2864, 3444
BmiI GGNNCC 7 cut(s) 565, 1289, 1615, 1908, 3203, 3413, 3613
BmrFI CCNGG 6 cut(s) 318, 1911, 2235, 2669, 3443, 3554
BmrI ACTGGG 5 cut(s) 182, 647, 943, 2099, 2857
BmsI GCATC 5 cut(s) 698, 777, 1773, 2636, 3242
BmtI GCTAGC 1 cut(s) 869
BmuI ACTGGG 5 cut(s) 182, 647, 943, 2099, 2857
BpiI GAAGAC 5 cut(s) 936, 1145, 1908, 2850, 3070
BpmI CTGGAG 6 cut(s) 312, 339, 751, 1491, 2391, 2496
Bpu10I CCTNAGC 2 cut(s) 1864, 3423
BpuEI CTTGAG 4 cut(s) 962, 1694, 2876, 3608
BpuMI CCSGG 3 cut(s) 2235, 3443, 3554
BsaAI YACGTR 2 cut(s) 1859, 1983
BsaHI GRCGYC 1 cut(s) 1150
BsaI GGTCTC 5 cut(s) 773, 1096, 1853, 2060, 3720
BsaWI WCCGGW 2 cut(s) 1161, 1995
Bse118I RCCGGY 1 cut(s) 1267
Bse3DI GCAATG 5 cut(s) 294, 1150, 2211, 2745, 3064
BseBI CCWGG 3 cut(s) 318, 1911, 2669
BseGI GGATG 5 cut(s) 119, 689, 1119, 1788, 3033
BseMI GCAATG 5 cut(s) 294, 1150, 2211, 2745, 3064
BseMII CTCAG 7 cut(s) 125, 968, 2385, 2976, 3414, 3683, 3876
BseRI GAGGAG 3 cut(s) 1730, 1843, 3644
BseSI GKGCMC 1 cut(s) 3821
BseYI CCCAGC 1 cut(s) 490
BsgI GTGCAG 3 cut(s) 1322, 1817, 3731
Bsh1236I CGCG 2 cut(s) 2557, 2606
Bsh1285I CGRYCG 1 cut(s) 1268
BshNI GGYRCC 2 cut(s) 563, 3411
BsiEI CGRYCG 1 cut(s) 1268
BsiHKAI GWGCWC 2 cut(s) 3429, 3458
BsiHKCI CYCGRG 2 cut(s) 1063, 3653
BsiSI CCGG 8 cut(s) 495, 1162, 1268, 1996, 2235, 3442, 3553, 3740
BsmBI CGTCTC 1 cut(s) 3512
BsmI GAATGC 1 cut(s) 3832
Bso31I GGTCTC 5 cut(s) 773, 1096, 1853, 2060, 3720
BsoBI CYCGRG 2 cut(s) 1063, 3653
Bsp1286I GDGCHC 3 cut(s) 3429, 3458, 3821
Bsp1407I TGTACA 2 cut(s) 672, 2589
Bsp19I CCATGG 2 cut(s) 277, 1987
BspACI CCGC 8 cut(s) 687, 858, 2070, 2604, 2711, 2831, 3771, 3871
BspCNI CTCAG 7 cut(s) 126, 967, 2386, 2977, 3415, 3682, 3875
BspFNI CGCG 2 cut(s) 2557, 2606
BspLI GGNNCC 7 cut(s) 565, 1289, 1615, 1908, 3203, 3413, 3613
BspMAI CTGCAG 2 cut(s) 1831, 3685
BspOI GCTAGC 1 cut(s) 869
BspPI GGATC 4 cut(s) 1359, 1679, 3273, 3593
BspT107I GGYRCC 2 cut(s) 563, 3411
BspTNI GGTCTC 5 cut(s) 773, 1096, 1853, 2060, 3720
BsrDI GCAATG 5 cut(s) 294, 1150, 2211, 2745, 3064
BsrFI RCCGGY 1 cut(s) 1267
BsrGI TGTACA 2 cut(s) 672, 2589
BssAI RCCGGY 1 cut(s) 1267
BssNAI GTATAC 1 cut(s) 886
BssNI GRCGYC 1 cut(s) 1150
BssSI CACGAG 3 cut(s) 523, 639, 3011
BssT1I CCWWGG 7 cut(s) 70, 271, 277, 1778, 1987, 2257, 3754
Bst1107I GTATAC 1 cut(s) 886
Bst2BI CACGAG 3 cut(s) 523, 639, 3011
Bst2UI CCWGG 3 cut(s) 318, 1911, 2669
Bst4CI ACNGT 5 cut(s) 154, 749, 1072, 2137, 3187
Bst6I CTCTTC 5 cut(s) 238, 1079, 2331, 2670, 2993
BstACI GRCGYC 1 cut(s) 1150
BstAPI GCANNNNNTGC 1 cut(s) 538
BstAUI TGTACA 2 cut(s) 672, 2589
BstBAI YACGTR 2 cut(s) 1859, 1983
BstC8I GCNNGC 9 cut(s) 836, 867, 1437, 1827, 2618, 3351, 3681, 3698, 3769
BstDSI CCRYGG 4 cut(s) 277, 1453, 1987, 3367
BstENI CCTNNNNNAGG 2 cut(s) 2712, 3502
BstF5I GGATG 5 cut(s) 119, 689, 1119, 1788, 3033
BstFNI CGCG 2 cut(s) 2557, 2606
BstHHI GCGC 4 cut(s) 1460, 1788, 3374, 3702
BstMCI CGRYCG 1 cut(s) 1268
BstNI CCWGG 3 cut(s) 318, 1911, 2669
BstNSI RCATGY 3 cut(s) 1231, 3145, 3700
BstSCI CCNGG 6 cut(s) 316, 1909, 2233, 2667, 3441, 3552
BstSFI CTRYAG 4 cut(s) 393, 1827, 2310, 3681
BstSLI GKGCMC 1 cut(s) 3821
BstUI CGCG 2 cut(s) 2557, 2606
BstV2I GAAGAC 5 cut(s) 936, 1145, 1908, 2850, 3070
BstX2I RGATCY 4 cut(s) 1684, 1969, 2729, 3598
BstXI CCANNNNNNTGG 2 cut(s) 278, 676
BstYI RGATCY 4 cut(s) 1684, 1969, 2729, 3598
BstZ17I GTATAC 1 cut(s) 886
BtgI CCRYGG 4 cut(s) 277, 1453, 1987, 3367
BtsCI GGATG 5 cut(s) 119, 689, 1119, 1788, 3033
BtsIMutI CAGTG 5 cut(s) 433, 593, 693, 1926, 3456
Cac8I GCNNGC 9 cut(s) 836, 867, 1437, 1827, 2618, 3351, 3681, 3698, 3769
CaiI CAGNNNCTG 2 cut(s) 47, 3680
CfoI GCGC 4 cut(s) 1460, 1788, 3374, 3702
Cfr10I RCCGGY 1 cut(s) 1267
Cfr13I GGNCC 6 cut(s) 215, 950, 1906, 2717, 2864, 3444
CseI GACGC 3 cut(s) 2563, 2595, 3529
DraI TTTAAA 2 cut(s) 964, 2878
DrdI GACNNNNNNGTC 2 cut(s) 446, 1155
DriI GACNNNNNGTC 1 cut(s) 1271
DseDI GACNNNNNNGTC 2 cut(s) 446, 1155
EaeI YGGCCR 4 cut(s) 2446, 2665, 3175, 3868
Eam1104I CTCTTC 5 cut(s) 238, 1079, 2331, 2670, 2993
Eam1105I GACNNNNNGTC 1 cut(s) 1271
EarI CTCTTC 5 cut(s) 238, 1079, 2331, 2670, 2993
Eco130I CCWWGG 7 cut(s) 70, 271, 277, 1778, 1987, 2257, 3754
Eco147I AGGCCT 1 cut(s) 2262
Eco31I GGTCTC 5 cut(s) 773, 1096, 1853, 2060, 3720
Eco32I GATATC 1 cut(s) 2177
Eco47I GGWCC 3 cut(s) 215, 2717, 2864
Eco57I CTGAAG 7 cut(s) 69, 777, 1902, 1986, 2072, 2694, 3828
Eco72I CACGTG 1 cut(s) 1859
Eco88I CYCGRG 2 cut(s) 1063, 3653
EcoNI CCTNNNNNAGG 2 cut(s) 2712, 3502
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 3 cut(s) 316, 1909, 2667
EcoRV GATATC 1 cut(s) 2177
EcoT14I CCWWGG 7 cut(s) 70, 271, 277, 1778, 1987, 2257, 3754
ErhI CCWWGG 7 cut(s) 70, 271, 277, 1778, 1987, 2257, 3754
Esp3I CGTCTC 1 cut(s) 3512
FalI AAGNNNNNCTT 2 cut(s) 1241, 1273
FauNDI CATATG 2 cut(s) 2635, 3838
FbaI TGATCA 2 cut(s) 519, 2191
FblI GTMKAC 1 cut(s) 885
FokI GGATG 5 cut(s) 106, 676, 1126, 1795, 3040
GlaI GCGC 4 cut(s) 1459, 1787, 3373, 3701
GsaI CCCAGC 1 cut(s) 494
GsuI CTGGAG 6 cut(s) 312, 339, 751, 1491, 2391, 2496
HapII CCGG 8 cut(s) 495, 1162, 1268, 1996, 2235, 3442, 3553, 3740
HgaI GACGC 3 cut(s) 2563, 2595, 3529
HhaI GCGC 4 cut(s) 1460, 1788, 3374, 3702
Hin1I GRCGYC 1 cut(s) 1150
Hin6I GCGC 4 cut(s) 1458, 1786, 3372, 3700
HinP1I GCGC 4 cut(s) 1458, 1786, 3372, 3700
HincII GTYRAC 1 cut(s) 3550
HindII GTYRAC 1 cut(s) 3550
HindIII AAGCTT 4 cut(s) 1736, 2054, 2618, 2693
HpaII CCGG 8 cut(s) 495, 1162, 1268, 1996, 2235, 3442, 3553, 3740
HphI GGTGA 5 cut(s) 55, 1151, 2146, 3065, 3741
Hpy166II GTNNAC 8 cut(s) 553, 886, 1042, 1366, 2470, 2610, 2801, 3550
Hpy8I GTNNAC 8 cut(s) 553, 886, 1042, 1366, 2470, 2610, 2801, 3550
Hpy99I CGWCG 1 cut(s) 3187
HpyCH4III ACNGT 5 cut(s) 154, 749, 1072, 2137, 3187
HpyCH4IV ACGT 4 cut(s) 1150, 1663, 1858, 1982
HpySE526I ACGT 4 cut(s) 1150, 1663, 1858, 1982
Hsp92I GRCGYC 1 cut(s) 1150
HspAI GCGC 4 cut(s) 1458, 1786, 3372, 3700
KpnI GGTACC 2 cut(s) 567, 3415
Ksp22I TGATCA 2 cut(s) 519, 2191
LweI GCATC 5 cut(s) 698, 777, 1773, 2636, 3242
MaeII ACGT 4 cut(s) 1150, 1663, 1858, 1982
MfeI CAATTG 1 cut(s) 3196
MflI RGATCY 4 cut(s) 1684, 1969, 2729, 3598
MhlI GDGCHC 3 cut(s) 3429, 3458, 3821
MlsI TGGCCA 3 cut(s) 2448, 2667, 3177
MluI ACGCGT 1 cut(s) 2555
MluNI TGGCCA 3 cut(s) 2448, 2667, 3177
MlyI GAGTC 4 cut(s) 631, 3014, 3023, 3857
MmeI TCCRAC 5 cut(s) 326, 398, 2243, 2404, 3548
Mox20I TGGCCA 3 cut(s) 2448, 2667, 3177
MroXI GAANNNNTTC 1 cut(s) 3035
MscI TGGCCA 3 cut(s) 2448, 2667, 3177
MseI TTAA 8 cut(s) 462, 714, 738, 963, 2379, 2577, 2655, 2877
MslI CAYNNNNRTG 5 cut(s) 674, 1371, 3285, 3461, 3837
Msp20I TGGCCA 3 cut(s) 2448, 2667, 3177
MspA1I CMGCKG 3 cut(s) 47, 3771, 3873
MspI CCGG 8 cut(s) 495, 1162, 1268, 1996, 2235, 3442, 3553, 3740
MspR9I CCNGG 6 cut(s) 318, 1911, 2235, 2669, 3443, 3554
MunI CAATTG 1 cut(s) 3196
Mva1269I GAATGC 1 cut(s) 3832
MvaI CCWGG 3 cut(s) 318, 1911, 2669
MvnI CGCG 2 cut(s) 2557, 2606
NciI CCSGG 3 cut(s) 2235, 3443, 3554
NcoI CCATGG 2 cut(s) 277, 1987
NdeI CATATG 2 cut(s) 2635, 3838
NheI GCTAGC 1 cut(s) 865
NlaIV GGNNCC 7 cut(s) 565, 1289, 1615, 1908, 3203, 3413, 3613
NspI RCATGY 3 cut(s) 1231, 3145, 3700
OliI CACNNNNGTG 1 cut(s) 3461
PaeI GCATGC 1 cut(s) 3700
PasI CCCWGGG 1 cut(s) 1910
PceI AGGCCT 1 cut(s) 2262
PctI GAATGC 1 cut(s) 3832
PdmI GAANNNNTTC 1 cut(s) 3035
PfeI GAWTC 7 cut(s) 282, 379, 1100, 1940, 2005, 3277, 3527
PflFI GACNNNGTC 1 cut(s) 3069
PleI GAGTC 4 cut(s) 631, 3013, 3022, 3856
PmaCI CACGTG 1 cut(s) 1859
PmlI CACGTG 1 cut(s) 1859
PpsI GAGTC 4 cut(s) 631, 3013, 3022, 3856
Ppu21I YACGTR 2 cut(s) 1859, 1983
PshBI ATTAAT 3 cut(s) 462, 714, 2379
Psp6I CCWGG 3 cut(s) 316, 1909, 2667
PspCI CACGTG 1 cut(s) 1859
PspFI CCCAGC 1 cut(s) 490
PspGI CCWGG 3 cut(s) 316, 1909, 2667
PspN4I GGNNCC 7 cut(s) 565, 1289, 1615, 1908, 3203, 3413, 3613
PspPI GGNCC 6 cut(s) 215, 950, 1906, 2717, 2864, 3444
PstI CTGCAG 2 cut(s) 1831, 3685
PstNI CAGNNNCTG 2 cut(s) 47, 3680
PsuI RGATCY 4 cut(s) 1684, 1969, 2729, 3598
PsyI GACNNNGTC 1 cut(s) 3069
PvuII CAGCTG 1 cut(s) 47
RseI CAYNNNNRTG 5 cut(s) 674, 1371, 3285, 3461, 3837
SaqAI TTAA 8 cut(s) 462, 714, 738, 963, 2379, 2577, 2655, 2877
Sau96I GGNCC 6 cut(s) 215, 950, 1906, 2717, 2864, 3444
ScaI AGTACT 1 cut(s) 3716
SchI GAGTC 4 cut(s) 631, 3014, 3023, 3857
ScrFI CCNGG 6 cut(s) 318, 1911, 2235, 2669, 3443, 3554
SduI GDGCHC 3 cut(s) 3429, 3458, 3821
SfaNI GCATC 5 cut(s) 698, 777, 1773, 2636, 3242
SfcI CTRYAG 4 cut(s) 393, 1827, 2310, 3681
SinI GGWCC 3 cut(s) 215, 2717, 2864
SmiMI CAYNNNNRTG 5 cut(s) 674, 1371, 3285, 3461, 3837
SmlI CTYRAG 4 cut(s) 941, 1673, 2855, 3587
SmoI CTYRAG 4 cut(s) 941, 1673, 2855, 3587
SphI GCATGC 1 cut(s) 3700
SseBI AGGCCT 1 cut(s) 2262
SsiI CCGC 8 cut(s) 687, 858, 2070, 2604, 2711, 2831, 3771, 3871
SspI AATATT 2 cut(s) 1390, 1669
StuI AGGCCT 1 cut(s) 2262
StyD4I CCNGG 6 cut(s) 316, 1909, 2233, 2667, 3441, 3552
StyI CCWWGG 7 cut(s) 70, 271, 277, 1778, 1987, 2257, 3754
TaaI ACNGT 5 cut(s) 154, 749, 1072, 2137, 3187
TaiI ACGT 4 cut(s) 1153, 1666, 1861, 1985
TaqI TCGA 5 cut(s) 411, 1281, 3159, 3312, 3516
TaqII GACCGA 1 cut(s) 1278
TatI WGTACW 6 cut(s) 672, 1224, 2589, 2800, 3138, 3714
TauI GCSGC 2 cut(s) 3774, 3873
TfiI GAWTC 7 cut(s) 282, 379, 1100, 1940, 2005, 3277, 3527
Tru1I TTAA 8 cut(s) 462, 714, 738, 963, 2379, 2577, 2655, 2877
Tru9I TTAA 8 cut(s) 462, 714, 738, 963, 2379, 2577, 2655, 2877
TscAI CASTG 5 cut(s) 433, 600, 700, 1926, 3463
TspDTI ATGAA 9 cut(s) 232, 525, 758, 1130, 1896, 1908, 3044, 3270, 3825
TspGWI ACGGA 2 cut(s) 2877, 3342
TspRI CASTG 5 cut(s) 433, 600, 700, 1926, 3463
Tth111I GACNNNGTC 1 cut(s) 3069
VpaK11BI GGWCC 3 cut(s) 215, 2717, 2864
VspI ATTAAT 3 cut(s) 462, 714, 2379
XagI CCTNNNNNAGG 2 cut(s) 2712, 3502
XapI RAATTY 4 cut(s) 422, 1165, 3617, 3806
XceI RCATGY 3 cut(s) 1231, 3145, 3700
XmaJI CCTAGG 1 cut(s) 2257
XmiI GTMKAC 1 cut(s) 885
XmnI GAANNNNTTC 1 cut(s) 3035
ZraI GACGTC 1 cut(s) 1151
ZrmI AGTACT 1 cut(s) 3716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.