Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0020828

Physical Location & Seq

Genomic Coordinates Reverse (-)

22508893 .. 22513380

Gene Structure 4488 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1146300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2094 bp

ATGCCGGTGAGAGTGGTGGAGACCTCCGCGCCTTCTCAACTTTCAGGTTCAAATTGTGGACAGACAACTCCTCCAGCTTGCACACTTTTAAGCGTTGGACAGAAAATGTTGGAAACTATGGGAAAATGGAGGGGTTGGAGGGATTGGAGTATGGGATTCAGCATAGCAGCTGCTGAAGATCTCAATTTCGCCTACCATGGTTTCGGGTCTGCAAATCTTAGCCTAGACGGCATAGCAAGAGTGACACCCAATGGTCTTCTGAGGCTTACAAATTACACTAGATTCAAGATTGGTCATGCCTTCTATCCCAACCCAGTAATTTTCAAGAACTCATCTAACGGTACAGTTCTCTCCTTCTCTACCACTTTCATCTTTGCTATGAGATATACAGGTCTCTATGGCCACGGAATAGCCTTTGTCATCGCTCCAACAAGAGGGCTGCCCCACGCTGCTCCAGCCCAACACCTAGGCCTTTTCAACTTTACAAACGATGGCAATCCAACAAATCATATTTTTGCTGTTGAGCTTGACACTTTTCGGAACGATGAGTTCAAGGACATCAATGATAACCATGTCGGGATTGATATTAATGGTTTGGCCTCTGAGGTGGCTTCTCCTGCCGGATATTATGTCGGAGAAAGATTGATCACCAGTGGCCAACCGGTACAAGTTTGGGTGGACTATGATGGTAGCAAGAAGCAAATCAATGTCTCTTTGGCTCTAGTTAGTAATGGTAAGCCCTATATTCCACTTTTGTCTTTGACACGCGACCTTTCCCCAGTTCTTCACAGAACCATGTACGTGGGCTTCTCCTCGTCCACTGGCCAGCTTACTGCTTCTGCTTACTTGTTGGGGTGGAGCTTTAAGATGAATGGTCAGGCTGAAGAACTTGTTCTCTCACAACTTCCCAAGATGCCTCGGCTAGGACCTAAAAAAATATCTAAACTTTTGACCATTGGTCTTCCTGTGATCTTGGTGAGTGTCGCTTTGCTAGCATTTCTTTTTTGTGTCAACCATGCCTTAAGAAGAAAGAAGAAGTTTGCAGAACTGCTTGAGGATTGGGAGCTTGAATATGGGCCTCAAAGGTTTAAATACAAAGAGTTATATATTGCCACAAAAGGGTTTCGAGAAGAGGAACTTTTGGGAAAGGGTGGATTCGGTAAGGTTTATAAAGGTATATTGCCCACCTCTGGGATTGAGATTGCTGTGAAGAGGGTCTCACACGAATCAAGACAGGGGATGAAGGAGTTTGTGGCAGAAATTGTCAGCAATGGACGTCTTCGTCACCGAAATTTAGTCCCATTATTGGGCTACTGCAGGAGAAAAGGAGAGCTATTGTTGGTTTATGAGTACATGCCTAATGGAAGCCTGGACAAGTACCTCTTTGGCCAACCGGCGGTTACTCTCGATTGGAGGCAAAGAGTTAGAGTCATAAGAGGTGTAGCATCAGGGTTGCTTTATTTGCATGAAGAATGGGATCAGGTTGTGGTTCACAGAGATGTCAAGGCCAGCAATGTATTACTAGATGGGGAATGGAATGGAAGACTAGGAGATTTTGGGCTTGCAAGATTATATGACCACGGCTCAGACCCTCAAACTACTCATATAGCTGGAACATTTGGGTATCTAGCTCCTGAGCATGCAAGATCAGGTCGGGCCACAACAAGCAGTGATGTGTTTGCTTTCGGTGTATTTTTGCTTGAAGTTGCAAGTGGAAGAAGGCCAATAGAGTATCATGGCTCTGAGGATGTGATTTTGGTTGATTGGGTCTTTTCTTGTTGGAAAGGAAGTAATATTCTTGAGGCGAAAGACCCAAAGTTAGGTAATGATTTTGTAGCGGAGGAGGTGGAGTTGGTGTTGAAGCTTGGGCTGTTGTGCTGCCAATCAGAGCCTACAGCAAGGCCAAGGATGCGCCAAGTTGTGCAGCATTTGGAGGGTGATATTCCTTTGCCTGCAGAGTTGTCACTTCTCAGTGCCTCTGCAAGTGGCCTAGCCATTTCTCAGAATGAAGGTTTTGATGAGTCTACAATGGCCTGGGGGTCTTCACCATCATCATATGCTGCAGATTCGTCATTCCTCTACAGTGGTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0010033 GO:0014070 GO:0016020 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0044464 GO:0046677 GO:0048229 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

698

Amino Acids

76.93

Weight (kDa)

8.02

Isoelectric Point (pI)

35.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	61 - 302	1.7e-78	Legume lectin domain
Lectin_L-type_dom	PF18483	83 - 280	1.7e-06	Legume lectin beta-barrel domain
Pkinase	PF00069	376 - 643	4.1e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	378 - 644	9.7e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0020828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37710
malus_domestica	MD04G1133500.v1.1 MD12G1146300.v1.1 MD12G1146500.v1.1
pyrus_communis	pycom12g14020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1170
AasI	GACNNNNNNGTC	1 cut(s)	1281
AatII	GACGTC	1 cut(s)	1279
AccI	GTMKAC	1 cut(s)	2024
AccII	CGCG	2 cut(s)	29, 768
AciI	CCGC	3 cut(s)	27, 1397, 1838
AclWI	GGATC	1 cut(s)	1485
AcoI	YGGCCR	4 cut(s)	400, 655, 823, 1387
AcsI	RAATTY	1 cut(s)	1291
AcuI	CTGAAG	2 cut(s)	195, 903
AcyI	GRCGYC	1 cut(s)	1276
AfaI	GTAC	5 cut(s)	343, 666, 800, 1352, 1379
AfiI	CCNNNNNNNGG	9 cut(s)	434, 923, 1119, 1190, 1191, 1306, 1307, 1396, 1820
AflII	CTTAAG	1 cut(s)	1021
AgeI	ACCGGT	1 cut(s)	661
AgsI	TTSAA	8 cut(s)	51, 286, 325, 478, 553, 1070, 1703, 1861
AhdI	GACNNNNNGTC	1 cut(s)	957
AjnI	CCWGG	2 cut(s)	1368, 2033
Alw26I	GTCTC	4 cut(s)	14, 398, 715, 1222
AlwI	GGATC	1 cut(s)	1485
AlwNI	CAGNNNCTG	1 cut(s)	173
ApeKI	GCWGC	7 cut(s)	167, 170, 439, 449, 1878, 1924, 2060
ApoI	RAATTY	1 cut(s)	1291
AseI	ATTAAT	1 cut(s)	588
AsiGI	ACCGGT	1 cut(s)	661
Asp700I	GAANNNNTTC	1 cut(s)	891
AspA2I	CCTAGG	1 cut(s)	466
AspLEI	GCGC	2 cut(s)	31, 1914
AspS9I	GGNCC	3 cut(s)	926, 1076, 1656
AsuHPI	GGTGA	6 cut(s)	19, 640, 988, 1277, 1949, 2037
AsuNHI	GCTAGC	1 cut(s)	991
AvaII	GGWCC	1 cut(s)	926
AvrII	CCTAGG	1 cut(s)	466
BalI	TGGCCA	4 cut(s)	402, 657, 825, 1389
BarI	GAAGNNNNNNTAC	2 cut(s)	791, 823
BbsI	GAAGAC	5 cut(s)	248, 953, 1271, 1549, 2034
BbvI	GCAGC	7 cut(s)	157, 179, 426, 436, 1865, 1936, 2047
BccI	CCATC	4 cut(s)	485, 680, 1520, 2056
BceAI	ACGGC	2 cut(s)	244, 1597
BciT130I	CCWGG	2 cut(s)	1370, 2035
BclI	TGATCA	1 cut(s)	645
BcoDI	GTCTC	4 cut(s)	14, 398, 715, 1222
BfmI	CTRYAG	5 cut(s)	1315, 1893, 1953, 2061, 2080
BfrI	CTTAAG	1 cut(s)	1021
BglI	GCCNNNNNGGC	1 cut(s)	228
BglII	AGATCT	1 cut(s)	178
BisI	GCNGC	7 cut(s)	168, 171, 440, 450, 1879, 1925, 2061
BlnI	CCTAGG	1 cut(s)	466
BlsI	GCNGC	7 cut(s)	169, 172, 441, 451, 1880, 1926, 2062
Bme1390I	CCNGG	2 cut(s)	1370, 2035
Bme18I	GGWCC	1 cut(s)	926
BmeRI	GACNNNNNGTC	1 cut(s)	957
BmgT120I	GGNCC	3 cut(s)	926, 1076, 1656
BmrFI	CCNGG	2 cut(s)	1370, 2035
BmrI	ACTGGG	2 cut(s)	308, 773
BmsI	GCATC	3 cut(s)	903, 1454, 1899
BmtI	GCTAGC	1 cut(s)	995
BmuI	ACTGGG	2 cut(s)	308, 773
BpiI	GAAGAC	5 cut(s)	248, 953, 1271, 1549, 2034
BpmI	CTGGAG	2 cut(s)	57, 438
Bpu10I	CCTNAGC	1 cut(s)	1635
BpuEI	CTTGAG	2 cut(s)	1073, 1820
BsaAI	YACGTR	1 cut(s)	802
BsaBI	GATNNNNATC	1 cut(s)	495
BsaHI	GRCGYC	1 cut(s)	1276
BsaI	GGTCTC	3 cut(s)	14, 398, 1222
BsaJI	CCNNGG	7 cut(s)	196, 403, 466, 917, 1579, 1904, 2034
BsaWI	WCCGGW	1 cut(s)	661
BsaXI	ACNNNNNCTCC	6 cut(s)	55, 85, 1835, 1841, 1865, 1871
Bsc4I	CCNNNNNNNGG	9 cut(s)	434, 923, 1119, 1190, 1191, 1306, 1307, 1396, 1820
Bse118I	RCCGGY	3 cut(s)	4, 661, 1393
Bse1I	ACTGG	4 cut(s)	314, 651, 779, 826
Bse3DI	GCAATG	2 cut(s)	1276, 1519
Bse8I	GATNNNNATC	1 cut(s)	495
BseBI	CCWGG	2 cut(s)	1370, 2035
BseDI	CCNNGG	7 cut(s)	196, 403, 466, 917, 1579, 1904, 2034
BseGI	GGATG	3 cut(s)	1245, 1753, 1914
BseJI	GATNNNNATC	1 cut(s)	495
BseLI	CCNNNNNNNGG	9 cut(s)	434, 923, 1119, 1190, 1191, 1306, 1307, 1396, 1820
BseMI	GCAATG	2 cut(s)	1276, 1519
BseMII	CTCAG	7 cut(s)	251, 594, 1599, 1626, 1734, 1984, 2015
BseNI	ACTGG	4 cut(s)	314, 651, 779, 826
BseRI	GAGGAG	3 cut(s)	60, 802, 1856
BseXI	GCAGC	7 cut(s)	157, 179, 426, 436, 1865, 1936, 2047
BsgI	GTGCAG	1 cut(s)	1943
Bsh1236I	CGCG	2 cut(s)	29, 768
BshTI	ACCGGT	1 cut(s)	661
BsiSI	CCGG	4 cut(s)	5, 621, 662, 1394
BslFI	GGGAC	1 cut(s)	1283
BslI	CCNNNNNNNGG	9 cut(s)	434, 923, 1119, 1190, 1191, 1306, 1307, 1396, 1820
BsmAI	GTCTC	4 cut(s)	14, 398, 715, 1222
BsmFI	GGGAC	1 cut(s)	1283
Bso31I	GGTCTC	3 cut(s)	14, 398, 1222
Bsp143I	GATC	5 cut(s)	178, 645, 969, 1477, 1646
Bsp19I	CCATGG	1 cut(s)	196
BspACI	CCGC	3 cut(s)	27, 1397, 1838
BspCNI	CTCAG	7 cut(s)	252, 595, 1598, 1627, 1735, 1983, 2014
BspFNI	CGCG	2 cut(s)	29, 768
BspMAI	CTGCAG	3 cut(s)	1319, 1957, 2065
BspOI	GCTAGC	1 cut(s)	995
BspPI	GGATC	1 cut(s)	1485
BspTI	CTTAAG	1 cut(s)	1021
BspTNI	GGTCTC	3 cut(s)	14, 398, 1222
BsrDI	GCAATG	2 cut(s)	1276, 1519
BsrFI	RCCGGY	3 cut(s)	4, 661, 1393
BsrI	ACTGG	4 cut(s)	314, 651, 779, 826
BssAI	RCCGGY	3 cut(s)	4, 661, 1393
BssECI	CCNNGG	7 cut(s)	196, 403, 466, 917, 1579, 1904, 2034
BssMI	GATC	5 cut(s)	178, 645, 969, 1477, 1646
BssNI	GRCGYC	1 cut(s)	1276
BssT1I	CCWWGG	3 cut(s)	196, 466, 1904
Bst2UI	CCWGG	2 cut(s)	1370, 2035
Bst4CI	ACNGT	3 cut(s)	341, 346, 2084
Bst6I	CTCTTC	2 cut(s)	1125, 1205
BstACI	GRCGYC	1 cut(s)	1276
BstAFI	CTTAAG	1 cut(s)	1021
BstBAI	YACGTR	1 cut(s)	802
BstC8I	GCNNGC	7 cut(s)	79, 827, 993, 1510, 1563, 1641, 1953
BstDEI	CTNAG	8 cut(s)	218, 260, 603, 1585, 1635, 1743, 1970, 2001
BstDSI	CCRYGG	3 cut(s)	196, 403, 1579
BstENI	CCTNNNNNAGG	1 cut(s)	921
BstF5I	GGATG	3 cut(s)	1245, 1753, 1914
BstFNI	CGCG	2 cut(s)	29, 768
BstHHI	GCGC	2 cut(s)	31, 1914
BstKTI	GATC	5 cut(s)	181, 648, 972, 1480, 1649
BstMAI	GTCTC	4 cut(s)	14, 398, 715, 1222
BstMBI	GATC	5 cut(s)	178, 645, 969, 1477, 1646
BstMWI	GCNNNNNNNGC	6 cut(s)	228, 455, 617, 992, 1462, 1909
BstNI	CCWGG	2 cut(s)	1370, 2035
BstNSI	RCATGY	2 cut(s)	1357, 1643
BstSCI	CCNGG	2 cut(s)	1368, 2033
BstSFI	CTRYAG	5 cut(s)	1315, 1893, 1953, 2061, 2080
BstUI	CGCG	2 cut(s)	29, 768
BstV1I	GCAGC	7 cut(s)	157, 179, 426, 436, 1865, 1936, 2047
BstV2I	GAAGAC	5 cut(s)	248, 953, 1271, 1549, 2034
BstX2I	RGATCY	1 cut(s)	178
BstXI	CCANNNNNNTGG	1 cut(s)	802
BstYI	RGATCY	1 cut(s)	178
BtgI	CCRYGG	3 cut(s)	196, 403, 1579
BtgZI	GCGATG	1 cut(s)	406
BtsCI	GGATG	3 cut(s)	1245, 1753, 1914
BtsI	GCAGTG	1 cut(s)	1675
BtsIMutI	CAGTG	5 cut(s)	658, 819, 1675, 1978, 2089
Cac8I	GCNNGC	7 cut(s)	79, 827, 993, 1510, 1563, 1641, 1953
CaiI	CAGNNNCTG	1 cut(s)	173
CfoI	GCGC	2 cut(s)	31, 1914
Cfr10I	RCCGGY	3 cut(s)	4, 661, 1393
Cfr13I	GGNCC	3 cut(s)	926, 1076, 1656
Csp6I	GTAC	5 cut(s)	342, 665, 799, 1351, 1378
CspAI	ACCGGT	1 cut(s)	661
CviAII	CATG	9 cut(s)	197, 296, 572, 796, 1016, 1354, 1466, 1640, 1736
CviQI	GTAC	5 cut(s)	342, 665, 799, 1351, 1378
DdeI	CTNAG	8 cut(s)	218, 260, 603, 1585, 1635, 1743, 1970, 2001
DpnI	GATC	5 cut(s)	180, 647, 971, 1479, 1648
DpnII	GATC	5 cut(s)	178, 645, 969, 1477, 1646
DraI	TTTAAA	1 cut(s)	1090
DrdI	GACNNNNNNGTC	1 cut(s)	1281
DriI	GACNNNNNGTC	1 cut(s)	957
DseDI	GACNNNNNNGTC	1 cut(s)	1281
EaeI	YGGCCR	4 cut(s)	400, 655, 823, 1387
Eam1104I	CTCTTC	2 cut(s)	1125, 1205
Eam1105I	GACNNNNNGTC	1 cut(s)	957
EarI	CTCTTC	2 cut(s)	1125, 1205
Eco130I	CCWWGG	3 cut(s)	196, 466, 1904
Eco147I	AGGCCT	1 cut(s)	471
Eco31I	GGTCTC	3 cut(s)	14, 398, 1222
Eco47I	GGWCC	1 cut(s)	926
Eco57I	CTGAAG	2 cut(s)	195, 903
EcoNI	CCTNNNNNAGG	1 cut(s)	921
EcoO109I	RGGNCCY	1 cut(s)	926
EcoRII	CCWGG	2 cut(s)	1368, 2033
EcoT14I	CCWWGG	3 cut(s)	196, 466, 1904
ErhI	CCWWGG	3 cut(s)	196, 466, 1904
FaeI	CATG	9 cut(s)	200, 299, 575, 799, 1019, 1357, 1469, 1643, 1739
FalI	AAGNNNNNCTT	4 cut(s)	1122, 1154, 1367, 1399
FaqI	GGGAC	1 cut(s)	1283
FatI	CATG	9 cut(s)	196, 295, 571, 795, 1015, 1353, 1465, 1639, 1735
FauNDI	CATATG	1 cut(s)	2056
FbaI	TGATCA	1 cut(s)	645
FblI	GTMKAC	1 cut(s)	2024
Fnu4HI	GCNGC	7 cut(s)	168, 171, 440, 450, 1879, 1925, 2061
FokI	GGATG	3 cut(s)	1252, 1760, 1921
Fsp4HI	GCNGC	7 cut(s)	168, 171, 440, 450, 1879, 1925, 2061
GlaI	GCGC	2 cut(s)	30, 1913
GluI	GCNGC	7 cut(s)	168, 171, 440, 450, 1879, 1925, 2061
GsuI	CTGGAG	2 cut(s)	57, 438
HapII	CCGG	4 cut(s)	5, 621, 662, 1394
HhaI	GCGC	2 cut(s)	31, 1914
Hin1I	GRCGYC	1 cut(s)	1276
Hin1II	CATG	9 cut(s)	200, 299, 575, 799, 1019, 1357, 1469, 1643, 1739
Hin6I	GCGC	2 cut(s)	29, 1912
HinP1I	GCGC	2 cut(s)	29, 1912
HincII	GTYRAC	1 cut(s)	1012
HindII	GTYRAC	1 cut(s)	1012
HindIII	AAGCTT	1 cut(s)	1862
HinfI	GANTC	7 cut(s)	156, 282, 1155, 1226, 1428, 2021, 2066
HpaII	CCGG	4 cut(s)	5, 621, 662, 1394
HphI	GGTGA	6 cut(s)	19, 640, 988, 1277, 1949, 2037
Hpy166II	GTNNAC	6 cut(s)	59, 679, 819, 1012, 1492, 2025
Hpy188I	TCNGA	8 cut(s)	261, 540, 604, 635, 1588, 1744, 1888, 2004
Hpy188III	TCNNGA	8 cut(s)	286, 325, 577, 1127, 1230, 1406, 1634, 1799
Hpy8I	GTNNAC	6 cut(s)	59, 679, 819, 1012, 1492, 2025
HpyAV	CCTTC	6 cut(s)	42, 310, 364, 1237, 1713, 2003
HpyCH4III	ACNGT	3 cut(s)	341, 346, 2084
HpyCH4IV	ACGT	2 cut(s)	801, 1276
HpyF10VI	GCNNNNNNNGC	6 cut(s)	228, 455, 617, 992, 1462, 1909
HpyF3I	CTNAG	8 cut(s)	218, 260, 603, 1585, 1635, 1743, 1970, 2001
HpySE526I	ACGT	2 cut(s)	801, 1276
Hsp92I	GRCGYC	1 cut(s)	1276
Hsp92II	CATG	9 cut(s)	200, 299, 575, 799, 1019, 1357, 1469, 1643, 1739
HspAI	GCGC	2 cut(s)	29, 1912
Ksp22I	TGATCA	1 cut(s)	645
Kzo9I	GATC	5 cut(s)	178, 645, 969, 1477, 1646
LmnI	GCTCC	5 cut(s)	430, 457, 858, 1063, 1636
Lsp1109I	GCAGC	7 cut(s)	157, 179, 426, 436, 1865, 1936, 2047
LweI	GCATC	3 cut(s)	903, 1454, 1899
MaeII	ACGT	2 cut(s)	801, 1276
MaeIII	GTNAC	4 cut(s)	241, 1283, 1399, 1962
MalI	GATC	5 cut(s)	180, 647, 971, 1479, 1648
MboI	GATC	5 cut(s)	178, 645, 969, 1477, 1646
MflI	RGATCY	1 cut(s)	178
MlsI	TGGCCA	4 cut(s)	402, 657, 825, 1389
MluCI	AATT	6 cut(s)	52, 184, 271, 318, 1260, 1291
MluNI	TGGCCA	4 cut(s)	402, 657, 825, 1389
MlyI	GAGTC	2 cut(s)	1437, 2030
MmeI	TCCRAC	7 cut(s)	76, 90, 116, 452, 524, 613, 1760
Mox20I	TGGCCA	4 cut(s)	402, 657, 825, 1389
MroXI	GAANNNNTTC	1 cut(s)	891
MscI	TGGCCA	4 cut(s)	402, 657, 825, 1389
MseI	TTAA	5 cut(s)	89, 588, 864, 1022, 1089
MslI	CAYNNNNRTG	2 cut(s)	800, 1497
Msp20I	TGGCCA	4 cut(s)	402, 657, 825, 1389
MspA1I	CMGCKG	1 cut(s)	170
MspCI	CTTAAG	1 cut(s)	1021
MspI	CCGG	4 cut(s)	5, 621, 662, 1394
MspR9I	CCNGG	2 cut(s)	1370, 2035
MvaI	CCWGG	2 cut(s)	1370, 2035
MvnI	CGCG	2 cut(s)	29, 768
MwoI	GCNNNNNNNGC	6 cut(s)	228, 455, 617, 992, 1462, 1909
NcoI	CCATGG	1 cut(s)	196
NdeI	CATATG	1 cut(s)	2056
NdeII	GATC	5 cut(s)	178, 645, 969, 1477, 1646
NheI	GCTAGC	1 cut(s)	991
NlaIII	CATG	9 cut(s)	200, 299, 575, 799, 1019, 1357, 1469, 1643, 1739
NmeAIII	GCCGAG	1 cut(s)	898
NmuCI	GTSAC	3 cut(s)	241, 1283, 1962
NspI	RCATGY	2 cut(s)	1357, 1643
PaeI	GCATGC	1 cut(s)	1643
PceI	AGGCCT	1 cut(s)	471
PdmI	GAANNNNTTC	1 cut(s)	891
PfeI	GAWTC	5 cut(s)	156, 282, 1155, 1226, 2066
PinAI	ACCGGT	1 cut(s)	661
PkrI	GCNGC	7 cut(s)	169, 172, 441, 451, 1880, 1926, 2062
PleI	GAGTC	2 cut(s)	1436, 2029
PpsI	GAGTC	2 cut(s)	1436, 2029
Ppu21I	YACGTR	1 cut(s)	802
PpuMI	RGGWCCY	1 cut(s)	926
PshBI	ATTAAT	1 cut(s)	588
PsiI	TTATAA	1 cut(s)	1170
Psp5II	RGGWCCY	1 cut(s)	926
Psp6I	CCWGG	2 cut(s)	1368, 2033
PspGI	CCWGG	2 cut(s)	1368, 2033
PspPI	GGNCC	3 cut(s)	926, 1076, 1656
PspPPI	RGGWCCY	1 cut(s)	926
PstI	CTGCAG	3 cut(s)	1319, 1957, 2065
PstNI	CAGNNNCTG	1 cut(s)	173
PsuI	RGATCY	1 cut(s)	178
PvuII	CAGCTG	1 cut(s)	170
RsaI	GTAC	5 cut(s)	343, 666, 800, 1352, 1379
RsaNI	GTAC	5 cut(s)	342, 665, 799, 1351, 1378
RseI	CAYNNNNRTG	2 cut(s)	800, 1497
SaqAI	TTAA	5 cut(s)	89, 588, 864, 1022, 1089
SatI	GCNGC	7 cut(s)	168, 171, 440, 450, 1879, 1925, 2061
Sau3AI	GATC	5 cut(s)	178, 645, 969, 1477, 1646
Sau96I	GGNCC	3 cut(s)	926, 1076, 1656
SchI	GAGTC	2 cut(s)	1437, 2030
ScrFI	CCNGG	2 cut(s)	1370, 2035
SfaNI	GCATC	3 cut(s)	903, 1454, 1899
SfcI	CTRYAG	5 cut(s)	1315, 1893, 1953, 2061, 2080
SinI	GGWCC	1 cut(s)	926
SmiMI	CAYNNNNRTG	2 cut(s)	800, 1497
SmlI	CTYRAG	3 cut(s)	1021, 1052, 1799
SmoI	CTYRAG	3 cut(s)	1021, 1052, 1799
SphI	GCATGC	1 cut(s)	1643
Sse9I	AATT	6 cut(s)	52, 184, 271, 318, 1260, 1291
SseBI	AGGCCT	1 cut(s)	471
SsiI	CCGC	3 cut(s)	27, 1397, 1838
SspI	AATATT	1 cut(s)	1795
StuI	AGGCCT	1 cut(s)	471
StyD4I	CCNGG	2 cut(s)	1368, 2033
StyI	CCWWGG	3 cut(s)	196, 466, 1904
TaaI	ACNGT	3 cut(s)	341, 346, 2084
TaiI	ACGT	2 cut(s)	804, 1279
TaqI	TCGA	3 cut(s)	1126, 1407, 2089
TasI	AATT	6 cut(s)	52, 184, 271, 318, 1260, 1291
TatI	WGTACW	1 cut(s)	1350
TfiI	GAWTC	5 cut(s)	156, 282, 1155, 1226, 2066
Tru1I	TTAA	5 cut(s)	89, 588, 864, 1022, 1089
Tru9I	TTAA	5 cut(s)	89, 588, 864, 1022, 1089
TscAI	CASTG	5 cut(s)	658, 826, 1675, 1978, 2089
TseFI	GTSAC	3 cut(s)	241, 1283, 1962
TseI	GCWGC	7 cut(s)	167, 170, 439, 449, 1878, 1924, 2060
Tsp45I	GTSAC	3 cut(s)	241, 1283, 1962
TspDTI	ATGAA	5 cut(s)	358, 884, 1256, 1482, 2022
TspGWI	ACGGA	1 cut(s)	420
TspRI	CASTG	5 cut(s)	658, 826, 1675, 1978, 2089
Vha464I	CTTAAG	1 cut(s)	1021
VpaK11BI	GGWCC	1 cut(s)	926
VspI	ATTAAT	1 cut(s)	588
XagI	CCTNNNNNAGG	1 cut(s)	921
XapI	RAATTY	1 cut(s)	1291
XceI	RCATGY	2 cut(s)	1357, 1643
XmaJI	CCTAGG	1 cut(s)	466
XmiI	GTMKAC	1 cut(s)	2024
XmnI	GAANNNNTTC	1 cut(s)	891
ZraI	GACGTC	1 cut(s)	1277

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.