Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008383

Physical Location & Seq

Genomic Coordinates Reverse (-)

25457284 .. 25459305

Gene Structure 2022 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1163200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2022 bp

ATGGCTTTACCAATTTCTTTAGCCTTCCTCCTCTTCTTTGCCATGTTTTCACCGGCTTGCGGAGTTGAGTTTTTGTTCAACGGATTCAATGGAAGTGAGGCAAGCGACCTAATCCGAGAGGGGGCGACTATATTCAAGCCCTCCGGCATGCTCAGGCTCACAAACGCATCACATAATATTATAGGCCATGCATTCTATCCCAAACCTATAAACTTTTTTGATAAAAACTCTTCTTCATACCCTAATGCTTCTTCCTTCAGCACACATTTTGTTTTTGCAATTGTCTCCCCTAGTTCCGGCCACGGAGGCTTTGGCCTAGCCTTCACACTCTCTCCCTCTCCAAAATTCCCTGGGGCGGAACCCGGGCATTTCTTTGGGATTTTCAACTCCACAACTGACAGTAACACCTCAAACCACATCCTTTCTGTGGAATTCGACACGGTGAATGGGTTTAAAGAGAACTCCAACACTGCAGGAAATCATGTTGGTATAAACAACAACAGTGTGTACTCTGTAGCTTCTGAACCAGCTGCTTACTCTGTCAAAGGAGCACACAAAGAAGAAGTAAACTTGGAAAGCGGTGAACTCATCCAAGCTTGGATAGACTACGATGGCAAAACTCAAATGCTGAATGTCACAATATCTCCCATACATGTGGAGAAACCTGAGAGACCACTATTGTCGCACCCTATCAATTTATCTACTACTCTTCAAGAGACTATGTACGCCGGCTTCTCTGCATCCACCGGAGAACAAGCGAGCTCTCATTACATATTAGCGTGGAGTTTCGCGGTGGATGGAATACCGAGACCAATCAATGTTTCGGAACTTCCTAAACCGCCAAAAGAGAAAGGTTCATCCTCTTACAGACCCGAAATCAAGGCTCTTATAATTGCCTTATCGGTTGTGGTTTGCGTGCTGTTGGGAGTTTTGTTGTTCTTCACCTTGTACAAAGGAATGCATGAGTTTGAGAGTCTTGAGGATTGGGAGCTGGATTGTCCTCACAGGTTCAAGTACAAGGATCTTTATGCAGCAACAAAGGGTTTCAAGGCCTCCGGTGTGATTGGAGCCGGCGGTTTTGGTGAAGTTTACAAGGGTGTCTTACCTTCTAGTGGATGTGAAATCGCGGTTAAGAAGATCACCCAAAATTCGATCCAAGGAATGAGGGAATTCGCTTCGGAAATTGAGAGCTTGGGAAAGTTGAGGCATAAGAACTTGGTCCATCTCCAGGGCTGGTGCAAGAAGAAGAGCGATCTCCTCATAGTCTACGATTACATCCCATATGGAAGCCTAGACTCGCTCATTTTTGGGTCCAAAAACAACTTTGTGCTGAGCTGGGCACAAAGGTTCAACATCCTCAAAGGCATTGCAACAGGGCTCTTGTACCTGCATGAGGAATGGGAGCAAGTGGTGATTCATCGCGATGTGAAGTCGAGCAATGTGTTAATAGATGCAGAAATGAACCCGAGGCTAAGCGACTTCGGCCTTGCCAGGCTGTATGATCATGGAGAAATATCACACACTACTGGCGTGGTTGGCACAATTGGGTATATTGCACCAGAATTGGCTCGCACAGGGAAGGCCTCCACATGCTCCGATGTGTTTGCATATGGAGTTCTACTCCTTGAAGTGGCTTGTGGGCAGAGACCTATCGGCTCAGGCGAGTTCATATTGGCGGATTGGGTGATGGAGAATCATGAAATGGATCACATACTCGATACAGTTGATCCGAACTTGGACTCGAATTTCGATGTTAAAGAGATGAAGTTGGTGCTGGAACTAGGTTTGCTTTGCACTCACTACAAACCAGAAGCTAGGCCTACCATGAGACAAGTGGTTCGATATCTCAATGAGGAGGATCAGCCTCCTGAAGTCGAAAACTGGAACCTCCTTGATTCGCGTCAGAACAGTGAATTGAACGCAAGATTTATGCAGGTTTTTTCGGTTGATACGATGGCATCGTATCCTTCATCGTCCTTGGGCGGGATCTCTTGCAGTTCCATGAACACCGGCAGATGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Lectin_legB Pkinase

Protein Analysis

674

Amino Acids

74.15

Weight (kDa)

5.61

Isoelectric Point (pI)

36.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	22 - 278	3.7e-77	Legume lectin domain
Lectin_L-type_dom	PF18483	42 - 256	8.6e-09	Legume lectin beta-barrel domain
Pkinase	PF00069	351 - 614	7.3e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	353 - 616	1.1e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008383)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08870 AT3G08870 AT5G01540 AT5G01550 AT5G01560
fragaria_vesca	FvH4_6g10960
malus_domestica	MD04G1163200.v1.1 MD12G1176700.v1.1
prunus_persica	Prupe.6G282700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0462261
rosa_laevigata	RLG00000024873 RLG00000032509
rosa_multiflora	Rmu_sc0006188.1_g000002 Rmu_sc0020622.1_g000003
rosa_roxburghii	Rroxscaffold_6G00417640
rosa_rugosa	Rorug03G0055200 Rorug03G0055200 Rorug03G0055300
rosa_samantha	Rh3AG114000 Rh3BG117100 Rh3CG119900 Rh3DG118900
rosa_wichuraiana	Rw3G009620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	890
Acc36I	ACCTGC	2 cut(s)	1395, 1924
AccI	GTMKAC	1 cut(s)	1266
AccII	CGCG	4 cut(s)	791, 1127, 1422, 1900
AciI	CCGC	9 cut(s)	60, 356, 579, 791, 839, 1074, 1127, 1676, 1983
AclWI	GGATC	6 cut(s)	1029, 1147, 1713, 1721, 1866, 1994
AcoI	YGGCCR	1 cut(s)	298
AcsI	RAATTY	5 cut(s)	344, 431, 1147, 1169, 1744
AcuI	CTGAAG	2 cut(s)	241, 1890
AfaI	GTAC	5 cut(s)	509, 725, 950, 1016, 1385
AflIII	ACRYGT	1 cut(s)	652
AjnI	CCWGG	3 cut(s)	349, 1227, 1490
AjuI	GAANNNNNNNTTGG	2 cut(s)	805, 837
AluBI	AGCT	8 cut(s)	518, 530, 596, 762, 991, 1191, 1335, 1814
AluI	AGCT	8 cut(s)	518, 530, 596, 762, 991, 1191, 1335, 1814
Alw21I	GWGCWC	2 cut(s)	553, 764
Alw26I	GTCTC	6 cut(s)	289, 664, 710, 802, 1639, 1822
AlwI	GGATC	6 cut(s)	1029, 1147, 1713, 1721, 1866, 1994
Ama87I	CYCGRG	2 cut(s)	362, 1465
AoxI	GGCC	7 cut(s)	184, 298, 313, 1050, 1483, 1581, 1817
ApeKI	GCWGC	2 cut(s)	530, 1031
ApoI	RAATTY	5 cut(s)	344, 431, 1147, 1169, 1744
ArsI	GACNNNNNNTTYG	4 cut(s)	1730, 1762, 1821, 1853
AspS9I	GGNCC	2 cut(s)	1219, 1311
AsuC2I	CCSGG	2 cut(s)	363, 364
AsuHPI	GGTGA	8 cut(s)	42, 454, 593, 934, 1094, 1132, 1423, 1696
AvaI	CYCGRG	2 cut(s)	362, 1465
AvaII	GGWCC	2 cut(s)	1219, 1311
BaeGI	GKGCMC	1 cut(s)	1342
BanII	GRGCYC	2 cut(s)	764, 1380
BarI	GAAGNNNNNNTAC	2 cut(s)	716, 748
Bbv12I	GWGCWC	2 cut(s)	553, 764
BbvI	GCAGC	2 cut(s)	517, 1043
BccI	CCATC	6 cut(s)	605, 791, 1230, 1681, 1948, 2010
BcgI	CGANNNNNNTGC	2 cut(s)	1586, 1620
BciT130I	CCWGG	3 cut(s)	351, 1229, 1492
BciVI	GTATCC	1 cut(s)	1974
BclI	TGATCA	1 cut(s)	1501
BcnI	CCSGG	2 cut(s)	363, 364
BcoDI	GTCTC	6 cut(s)	289, 664, 710, 802, 1639, 1822
BfaI	CTAG	6 cut(s)	291, 317, 1110, 1292, 1781, 1815
BfmI	CTRYAG	2 cut(s)	471, 513
BfuAI	ACCTGC	2 cut(s)	1395, 1924
BfuI	GTATCC	1 cut(s)	1974
BglI	GCCNNNNNGGC	1 cut(s)	306
BisI	GCNGC	2 cut(s)	531, 1032
BlpI	GCTNAGC	2 cut(s)	1331, 1472
BlsI	GCNGC	2 cut(s)	532, 1033
Bme1390I	CCNGG	5 cut(s)	351, 363, 364, 1229, 1492
Bme18I	GGWCC	2 cut(s)	1219, 1311
BmeT110I	CYCGRG	2 cut(s)	362, 1465
BmgT120I	GGNCC	2 cut(s)	1219, 1311
BmiI	GGNNCC	4 cut(s)	360, 1069, 1312, 1886
BmrFI	CCNGG	5 cut(s)	351, 363, 364, 1229, 1492
BmsI	GCATC	4 cut(s)	176, 749, 1441, 1967
BplI	GAGNNNNNCTC	2 cut(s)	1605, 1637
BpmI	CTGGAG	1 cut(s)	1211
Bpu10I	CCTNAGC	2 cut(s)	152, 1657
Bpu1102I	GCTNAGC	2 cut(s)	1331, 1472
BpuEI	CTTGAG	1 cut(s)	998
BpuMI	CCSGG	2 cut(s)	363, 364
BsaI	GGTCTC	3 cut(s)	664, 802, 1639
BsaJI	CCNNGG	8 cut(s)	301, 349, 350, 362, 1156, 1228, 1466, 1977
BsaWI	WCCGGW	2 cut(s)	746, 1055
BsaXI	ACNNNNNCTCC	4 cut(s)	316, 346, 628, 658
Bse118I	RCCGGY	4 cut(s)	52, 728, 1070, 2009
Bse1I	ACTGG	2 cut(s)	1531, 1886
Bse3DI	GCAATG	2 cut(s)	1365, 1444
BseBI	CCWGG	3 cut(s)	351, 1229, 1492
BseDI	CCNNGG	8 cut(s)	301, 349, 350, 362, 1156, 1228, 1466, 1977
BseGI	GGATG	8 cut(s)	417, 588, 740, 802, 857, 1121, 1275, 1353
BseMI	GCAATG	2 cut(s)	1365, 1444
BseMII	CTCAG	4 cut(s)	166, 657, 1322, 1671
BseNI	ACTGG	2 cut(s)	1531, 1886
BseRI	GAGGAG	3 cut(s)	20, 1247, 1868
BseSI	GKGCMC	1 cut(s)	1342
BseXI	GCAGC	2 cut(s)	517, 1043
BseYI	CCCAGC	1 cut(s)	1335
Bsh1236I	CGCG	4 cut(s)	791, 1127, 1422, 1900
BshFI	GGCC	7 cut(s)	186, 300, 315, 1052, 1485, 1583, 1819
BsiHKAI	GWGCWC	2 cut(s)	553, 764
BsiHKCI	CYCGRG	2 cut(s)	362, 1465
BsiSI	CCGG	9 cut(s)	53, 144, 297, 363, 729, 747, 1056, 1071, 2010
BsmAI	GTCTC	6 cut(s)	289, 664, 710, 802, 1639, 1822
BsmI	GAATGC	2 cut(s)	191, 963
BsnI	GGCC	7 cut(s)	186, 300, 315, 1052, 1485, 1583, 1819
Bso31I	GGTCTC	3 cut(s)	664, 802, 1639
BsoBI	CYCGRG	2 cut(s)	362, 1465
Bsp1286I	GDGCHC	4 cut(s)	553, 764, 1342, 1380
Bsp1407I	TGTACA	1 cut(s)	948
Bsp143I	GATC	9 cut(s)	1021, 1137, 1152, 1252, 1501, 1705, 1726, 1858, 1986
Bsp1720I	GCTNAGC	2 cut(s)	1331, 1472
Bsp68I	TCGCGA	1 cut(s)	1422
BspACI	CCGC	9 cut(s)	60, 356, 579, 791, 839, 1074, 1127, 1676, 1983
BspANI	GGCC	7 cut(s)	186, 300, 315, 1052, 1485, 1583, 1819
BspCNI	CTCAG	4 cut(s)	165, 658, 1323, 1670
BspFNI	CGCG	4 cut(s)	791, 1127, 1422, 1900
BspHI	TCATGA	1 cut(s)	1696
BspLI	GGNNCC	4 cut(s)	360, 1069, 1312, 1886
BspMAI	CTGCAG	1 cut(s)	475
BspMI	ACCTGC	2 cut(s)	1395, 1924
BspPI	GGATC	6 cut(s)	1029, 1147, 1713, 1721, 1866, 1994
BspQI	GCTCTTC	1 cut(s)	1241
BspTNI	GGTCTC	3 cut(s)	664, 802, 1639
BsrDI	GCAATG	2 cut(s)	1365, 1444
BsrFI	RCCGGY	4 cut(s)	52, 728, 1070, 2009
BsrGI	TGTACA	1 cut(s)	948
BsrI	ACTGG	2 cut(s)	1531, 1886
BssAI	RCCGGY	4 cut(s)	52, 728, 1070, 2009
BssECI	CCNNGG	8 cut(s)	301, 349, 350, 362, 1156, 1228, 1466, 1977
BssMI	GATC	9 cut(s)	1021, 1137, 1152, 1252, 1501, 1705, 1726, 1858, 1986
BssT1I	CCWWGG	2 cut(s)	1156, 1977
Bst2UI	CCWGG	3 cut(s)	351, 1229, 1492
Bst4CI	ACNGT	5 cut(s)	401, 442, 503, 1723, 1910
Bst6I	CTCTTC	4 cut(s)	38, 235, 714, 1241
BstAUI	TGTACA	1 cut(s)	948
BstC8I	GCNNGC	8 cut(s)	58, 103, 149, 730, 760, 917, 1072, 1570
BstDEI	CTNAG	5 cut(s)	152, 666, 1331, 1472, 1657
BstDSI	CCRYGG	1 cut(s)	301
BstENI	CCTNNNNNAGG	1 cut(s)	1391
BstF5I	GGATG	8 cut(s)	417, 588, 740, 802, 857, 1121, 1275, 1353
BstFNI	CGCG	4 cut(s)	791, 1127, 1422, 1900
BstKTI	GATC	9 cut(s)	1024, 1140, 1155, 1255, 1504, 1708, 1729, 1861, 1989
BstMAI	GTCTC	6 cut(s)	289, 664, 710, 802, 1639, 1822
BstMBI	GATC	9 cut(s)	1021, 1137, 1152, 1252, 1501, 1705, 1726, 1858, 1986
BstMWI	GCNNNNNNNGC	3 cut(s)	306, 1482, 1536
BstNI	CCWGG	3 cut(s)	351, 1229, 1492
BstNSI	RCATGY	3 cut(s)	151, 656, 1593
BstSCI	CCNGG	5 cut(s)	349, 361, 362, 1227, 1490
BstSFI	CTRYAG	2 cut(s)	471, 513
BstSLI	GKGCMC	1 cut(s)	1342
BstUI	CGCG	4 cut(s)	791, 1127, 1422, 1900
BstV1I	GCAGC	2 cut(s)	517, 1043
BstX2I	RGATCY	2 cut(s)	1021, 1986
BstXI	CCANNNNNNTGG	1 cut(s)	655
BstYI	RGATCY	2 cut(s)	1021, 1986
BsuI	GTATCC	1 cut(s)	1974
BsuRI	GGCC	7 cut(s)	186, 300, 315, 1052, 1485, 1583, 1819
BtgI	CCRYGG	1 cut(s)	301
BtgZI	GCGATG	2 cut(s)	1403, 1437
BtsCI	GGATG	8 cut(s)	417, 588, 740, 802, 857, 1121, 1275, 1353
BtsI	GCAGTG	1 cut(s)	468
BtsIMutI	CAGTG	3 cut(s)	468, 508, 1915
BtuMI	TCGCGA	1 cut(s)	1422
BveI	ACCTGC	2 cut(s)	1395, 1924
Cac8I	GCNNGC	8 cut(s)	58, 103, 149, 730, 760, 917, 1072, 1570
CciI	TCATGA	1 cut(s)	1696
Cfr10I	RCCGGY	4 cut(s)	52, 728, 1070, 2009
Cfr13I	GGNCC	2 cut(s)	1219, 1311
Cfr9I	CCCGGG	1 cut(s)	362
CseI	GACGC	1 cut(s)	1889
Csp6I	GTAC	5 cut(s)	508, 724, 949, 1015, 1384
CviQI	GTAC	5 cut(s)	508, 724, 949, 1015, 1384
DdeI	CTNAG	5 cut(s)	152, 666, 1331, 1472, 1657
DpnI	GATC	9 cut(s)	1023, 1139, 1154, 1254, 1503, 1707, 1728, 1860, 1988
DpnII	GATC	9 cut(s)	1021, 1137, 1152, 1252, 1501, 1705, 1726, 1858, 1986
DraI	TTTAAA	1 cut(s)	454
EaeI	YGGCCR	1 cut(s)	298
Eam1104I	CTCTTC	4 cut(s)	38, 235, 714, 1241
EarI	CTCTTC	4 cut(s)	38, 235, 714, 1241
EciI	GGCGGA	2 cut(s)	371, 1691
Ecl136II	GAGCTC	1 cut(s)	762
Eco130I	CCWWGG	2 cut(s)	1156, 1977
Eco147I	AGGCCT	3 cut(s)	1052, 1583, 1819
Eco24I	GRGCYC	2 cut(s)	764, 1380
Eco31I	GGTCTC	3 cut(s)	664, 802, 1639
Eco32I	GATATC	1 cut(s)	1844
Eco47I	GGWCC	2 cut(s)	1219, 1311
Eco53kI	GAGCTC	1 cut(s)	762
Eco57I	CTGAAG	2 cut(s)	241, 1890
Eco88I	CYCGRG	2 cut(s)	362, 1465
EcoICRI	GAGCTC	1 cut(s)	762
EcoNI	CCTNNNNNAGG	1 cut(s)	1391
EcoRI	GAATTC	2 cut(s)	431, 1169
EcoRII	CCWGG	3 cut(s)	349, 1227, 1490
EcoRV	GATATC	1 cut(s)	1844
EcoT14I	CCWWGG	2 cut(s)	1156, 1977
EcoT22I	ATGCAT	2 cut(s)	193, 963
EcoT38I	GRGCYC	2 cut(s)	764, 1380
ErhI	CCWWGG	2 cut(s)	1156, 1977
FalI	AAGNNNNNCTT	2 cut(s)	1085, 1117
FauI	CCCGC	1 cut(s)	1976
FauNDI	CATATG	2 cut(s)	1282, 1609
FbaI	TGATCA	1 cut(s)	1501
FblI	GTMKAC	1 cut(s)	1266
Fnu4HI	GCNGC	2 cut(s)	531, 1032
FokI	GGATG	8 cut(s)	404, 575, 727, 809, 844, 1128, 1262, 1340
FriOI	GRGCYC	2 cut(s)	764, 1380
Fsp4HI	GCNGC	2 cut(s)	531, 1032
FspBI	CTAG	6 cut(s)	291, 317, 1110, 1292, 1781, 1815
GluI	GCNGC	2 cut(s)	531, 1032
GsaI	CCCAGC	1 cut(s)	1339
GsuI	CTGGAG	1 cut(s)	1211
HaeIII	GGCC	7 cut(s)	186, 300, 315, 1052, 1485, 1583, 1819
HapII	CCGG	9 cut(s)	53, 144, 297, 363, 729, 747, 1056, 1071, 2010
HgaI	GACGC	1 cut(s)	1889
HindIII	AAGCTT	1 cut(s)	594
HinfI	GANTC	7 cut(s)	84, 973, 1295, 1414, 1693, 1739, 1895
HpaII	CCGG	9 cut(s)	53, 144, 297, 363, 729, 747, 1056, 1071, 2010
HphI	GGTGA	8 cut(s)	42, 454, 593, 934, 1094, 1132, 1423, 1696
Hpy166II	GTNNAC	5 cut(s)	508, 568, 584, 1090, 1267
Hpy188I	TCNGA	7 cut(s)	116, 523, 826, 1180, 1597, 1731, 1905
Hpy188III	TCNNGA	5 cut(s)	713, 977, 1421, 1697, 1868
Hpy8I	GTNNAC	5 cut(s)	508, 568, 584, 1090, 1267
HpyAV	CCTTC	6 cut(s)	34, 265, 331, 1116, 1573, 1977
HpyCH4III	ACNGT	5 cut(s)	401, 442, 503, 1723, 1910
HpyF10VI	GCNNNNNNNGC	3 cut(s)	306, 1482, 1536
HpyF3I	CTNAG	5 cut(s)	152, 666, 1331, 1472, 1657
KroI	GCCGGC	2 cut(s)	728, 1070
KroNI	GCCGGC	2 cut(s)	730, 1072
Ksp22I	TGATCA	1 cut(s)	1501
Kzo9I	GATC	9 cut(s)	1021, 1137, 1152, 1252, 1501, 1705, 1726, 1858, 1986
LguI	GCTCTTC	1 cut(s)	1241
LmnI	GCTCC	5 cut(s)	548, 988, 1067, 1402, 1598
Lsp1109I	GCAGC	2 cut(s)	517, 1043
LweI	GCATC	4 cut(s)	176, 749, 1441, 1967
MaeI	CTAG	6 cut(s)	291, 317, 1110, 1292, 1781, 1815
MaeIII	GTNAC	2 cut(s)	401, 634
MalI	GATC	9 cut(s)	1023, 1139, 1154, 1254, 1503, 1707, 1728, 1860, 1988
MboI	GATC	9 cut(s)	1021, 1137, 1152, 1252, 1501, 1705, 1726, 1858, 1986
MfeI	CAATTG	2 cut(s)	279, 1542
MflI	RGATCY	2 cut(s)	1021, 1986
MhlI	GDGCHC	4 cut(s)	553, 764, 1342, 1380
MlyI	GAGTC	3 cut(s)	982, 1289, 1733
MmeI	TCCRAC	1 cut(s)	489
Mph1103I	ATGCAT	2 cut(s)	193, 963
MroNI	GCCGGC	2 cut(s)	728, 1070
MseI	TTAA	4 cut(s)	453, 1131, 1445, 1755
MslI	CAYNNNNRTG	2 cut(s)	653, 1422
MspA1I	CMGCKG	1 cut(s)	530
MspI	CCGG	9 cut(s)	53, 144, 297, 363, 729, 747, 1056, 1071, 2010
MspR9I	CCNGG	5 cut(s)	351, 363, 364, 1229, 1492
MunI	CAATTG	2 cut(s)	279, 1542
Mva1269I	GAATGC	2 cut(s)	191, 963
MvaI	CCWGG	3 cut(s)	351, 1229, 1492
MvnI	CGCG	4 cut(s)	791, 1127, 1422, 1900
MwoI	GCNNNNNNNGC	3 cut(s)	306, 1482, 1536
NaeI	GCCGGC	2 cut(s)	730, 1072
NciI	CCSGG	2 cut(s)	363, 364
NdeI	CATATG	2 cut(s)	1282, 1609
NdeII	GATC	9 cut(s)	1021, 1137, 1152, 1252, 1501, 1705, 1726, 1858, 1986
NgoMIV	GCCGGC	2 cut(s)	728, 1070
NlaIV	GGNNCC	4 cut(s)	360, 1069, 1312, 1886
NmuCI	GTSAC	1 cut(s)	634
NruI	TCGCGA	1 cut(s)	1422
NsiI	ATGCAT	2 cut(s)	193, 963
NspI	RCATGY	3 cut(s)	151, 656, 1593
PaeI	GCATGC	1 cut(s)	151
PagI	TCATGA	1 cut(s)	1696
PasI	CCCWGGG	1 cut(s)	350
PceI	AGGCCT	3 cut(s)	1052, 1583, 1819
PciI	ACATGT	1 cut(s)	652
PciSI	GCTCTTC	1 cut(s)	1241
PcsI	WCGNNNNNNNCGW	3 cut(s)	1659, 1949, 1958
PctI	GAATGC	2 cut(s)	191, 963
PdiI	GCCGGC	2 cut(s)	730, 1072
PfeI	GAWTC	4 cut(s)	84, 1414, 1693, 1895
PkrI	GCNGC	2 cut(s)	532, 1033
PleI	GAGTC	3 cut(s)	981, 1289, 1733
PpsI	GAGTC	3 cut(s)	981, 1289, 1733
PscI	ACATGT	1 cut(s)	652
PsiI	TTATAA	1 cut(s)	890
Psp124BI	GAGCTC	1 cut(s)	764
Psp6I	CCWGG	3 cut(s)	349, 1227, 1490
PspFI	CCCAGC	1 cut(s)	1335
PspGI	CCWGG	3 cut(s)	349, 1227, 1490
PspN4I	GGNNCC	4 cut(s)	360, 1069, 1312, 1886
PspPI	GGNCC	2 cut(s)	1219, 1311
PstI	CTGCAG	1 cut(s)	475
PsuI	RGATCY	2 cut(s)	1021, 1986
PvuII	CAGCTG	1 cut(s)	530
RruI	TCGCGA	1 cut(s)	1422
RsaI	GTAC	5 cut(s)	509, 725, 950, 1016, 1385
RsaNI	GTAC	5 cut(s)	508, 724, 949, 1015, 1384
RseI	CAYNNNNRTG	2 cut(s)	653, 1422
SacI	GAGCTC	1 cut(s)	764
SapI	GCTCTTC	1 cut(s)	1241
SaqAI	TTAA	4 cut(s)	453, 1131, 1445, 1755
SatI	GCNGC	2 cut(s)	531, 1032
Sau3AI	GATC	9 cut(s)	1021, 1137, 1152, 1252, 1501, 1705, 1726, 1858, 1986
Sau96I	GGNCC	2 cut(s)	1219, 1311
SchI	GAGTC	3 cut(s)	982, 1289, 1733
ScrFI	CCNGG	5 cut(s)	351, 363, 364, 1229, 1492
SduI	GDGCHC	4 cut(s)	553, 764, 1342, 1380
SfaNI	GCATC	4 cut(s)	176, 749, 1441, 1967
SfcI	CTRYAG	2 cut(s)	471, 513
SinI	GGWCC	2 cut(s)	1219, 1311
SmaI	CCCGGG	1 cut(s)	364
SmiMI	CAYNNNNRTG	2 cut(s)	653, 1422
SmlI	CTYRAG	1 cut(s)	977
SmoI	CTYRAG	1 cut(s)	977
SphI	GCATGC	1 cut(s)	151
SseBI	AGGCCT	3 cut(s)	1052, 1583, 1819
SsiI	CCGC	9 cut(s)	60, 356, 579, 791, 839, 1074, 1127, 1676, 1983
SspI	AATATT	1 cut(s)	178
SspMI	CTAG	6 cut(s)	291, 317, 1110, 1292, 1781, 1815
SstI	GAGCTC	1 cut(s)	764
StuI	AGGCCT	3 cut(s)	1052, 1583, 1819
StyD4I	CCNGG	5 cut(s)	349, 361, 362, 1227, 1490
StyI	CCWWGG	2 cut(s)	1156, 1977
TaaI	ACNGT	5 cut(s)	401, 442, 503, 1723, 1910
TaqI	TCGA	8 cut(s)	435, 1151, 1433, 1716, 1742, 1749, 1840, 1875
TatI	WGTACW	3 cut(s)	507, 948, 1014
TfiI	GAWTC	4 cut(s)	84, 1414, 1693, 1895
Tru1I	TTAA	4 cut(s)	453, 1131, 1445, 1755
Tru9I	TTAA	4 cut(s)	453, 1131, 1445, 1755
TscAI	CASTG	3 cut(s)	475, 508, 1915
TseFI	GTSAC	1 cut(s)	634
TseI	GCWGC	2 cut(s)	530, 1031
Tsp45I	GTSAC	1 cut(s)	634
TspDTI	ATGAA	9 cut(s)	225, 846, 1406, 1475, 1657, 1713, 1778, 1959, 2018
TspGWI	ACGGA	2 cut(s)	96, 318
TspMI	CCCGGG	1 cut(s)	362
TspRI	CASTG	3 cut(s)	475, 508, 1915
VpaK11BI	GGWCC	2 cut(s)	1219, 1311
XagI	CCTNNNNNAGG	1 cut(s)	1391
XapI	RAATTY	5 cut(s)	344, 431, 1147, 1169, 1744
XceI	RCATGY	3 cut(s)	151, 656, 1593
XcmI	CCANNNNNNNNNTGG	2 cut(s)	308, 1831
XmaI	CCCGGG	1 cut(s)	362
XmiI	GTMKAC	1 cut(s)	1266
XspI	CTAG	6 cut(s)	291, 317, 1110, 1292, 1781, 1815
Zsp2I	ATGCAT	2 cut(s)	193, 963

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.