Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0017121

Physical Location & Seq

Genomic Coordinates Forward (+)

30463117 .. 30465069

Gene Structure 1953 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1224800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1953 bp

ATGCAGCAATGTGGGCGAGGCATTCGCTGCCTTTCGAGTTCGAGTTCAATGGGGAGGAGGATCCACTGGGATCCGACTGTTGCGCTCGAACTCAATCACCCGGCTCTTGTGTTGTTGGAGAAGTGCAGCACAAGGCACCATTTCAAGCAGATTTTGGGGCAGATGATGAGGACCTCTTTAATTGGTCAGACATTTCCCATGAGCCGGCTTCTCACATTCTCCGCCATCTCACACCCTGAAAATTTAGACATGGCCGTCCTCCTTTTCGATCACTTTACTCCGTATCCCAATCTTTACATTTATAATTCGATGATTTCTGCATTATCTTTCTCGAAAAGCCAAGCATTTGCTCTGTACAGTTCCATGCTCAAAGCTGGCATATACCCTGATAAACACACCCTTCTTTACTTGCTCCAAGCATCCGAATGTGTAGTGGAAGCAAAGCAGATCCACAGCCACGCTGTAGTTACAGGTTTGTCGTCACACCGGTACTTGCAGAACACCCTCATGAAGATATATCTGGAAAAAGGAGAGATGGGGCTTGCTCAACAGTTGTTTGATGACGTGCCAACGCCGGATACAGTCCCGTTCAATATCATGATCGTTGGTTATGCCAAGATGGGATATTTTTCGGAAGCTCTGCAACTGTTTTATGAAATGGTGGGTTTGGGTCTTGAGCCGGATGAGTTTACCATTCTAGGACTTCTTATATCTTGTGGACTATTAGGAGATGCTCGGCTTGGAATGTCTGTTCATGCATGGATTGAGAGGAAGAAGGCCATTACGTCTGGAAATTTGATTTTGGGTAATGCTCTTTTAGACATGTATGTGAAATGGAACAAATTGGAGGTTGCCCAAAGTCTTTTTGATGCTTTAGCGGAGAAGGATATCGTGTCCTGGAACACCATGGCTGCAGGATATGCCAAGGTTGGGAAAATAGAACTTGCGCGGAATTATTTTGAGCAAATGCCTAGAAGAGATCTTGTTTCTTGGAATTCATTAATTGCTGGATATGCCAAGAAGGGCGATTACACAATGGCGGTGAAGGTATTCAACGAAATGATTACAGAGAATGCAAAGCCTGACAGTATCACTCTGGTTAATTTGGTCTCTGCTGCAGCGGAAACTGGAGCGCTGGACCATGGAAGATCAATGCATGGCTTGATTGTGAGGATGCAGTTGAAAATAGATGTATTTTTGGGTTCGGCATTAATAGACATGTACTGCAAGGCCGGAAGCATTGAACGAGCCTTCATGGTTTTCCGGGGACTGACAGAGAAAGATGTTACCGTATGGACAACAATGATCTCTGGATTCGCATTCCATGGTTACGGGTGCAAAGCCCTGGATCTGTTTTCGGAGATGCAGGGAGGGCTATCTCCGAACGGGGTGACTCTTCTTGCTGTTCTTACAGCTTGTAGCCACAGCGGCCTTGTGGATGAAGGGCTTAATCTATTCAACAACATGGACAAAAAATTTGGTATTGAACCAGGAGTTGAGCACTATGGGTGTTTAGTGGACCTTTTATGTCGATCGGGAAGGCTGGCAGAGGCGAAAGAAGTGATAAAAAAGATGCCGGTAAGGCCAAGCCGAAACATTTGGGGAGCAATGCTGAGTGCTTGTAGAGCACAGGGAGACATGGAACTAGCCGAAACAGCTTCAGCAGAGCTACTCAAGTTAGAGCCGGGGAGAGAGGGCGGATACATTATGATGTCGAATATATACGCTGCTTGTGGAAAGTGGAGCTACTCGGATAAGATTCGAGAAGTAATGGAGATCAGGGGAGTGAAGAAGGCCGCAGGTTGCAGTAGCGTGAACGTCGACGGAGTTGTCCACAACTTTGTTGCCGCCGATAAGCGGCATCCGAGATGGGAAGAAATCTTCCTTGTTTTGCAGTGCATGAAAAGTGAAATGAAGCTGGCTGCTGGTTTCCGATTTGATCCATTTGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1900865 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

651

Amino Acids

72.17

Weight (kDa)

7.25

Isoelectric Point (pI)

40.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	192 - 235	5.7e-09	PPR repeat family
PPR	PF01535	196 - 225	4.2e-07	PPR repeat
PPR_2	PF13041	327 - 371	1.5e-11	PPR repeat family
PPR	PF01535	329 - 357	1.8e-08	PPR repeat
PPR_2	PF13041	427 - 474	1.4e-06	PPR repeat family
PPR_1	PF12854	494 - 525	1.7e-06	PPR repeat
E_motif	PF20431	546 - 605	9.1e-15	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0017121)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04750
malus_domestica	MD04G1224800.v1.1
prunus_persica	Prupe.6G343500_v2.0.a1
pyrus_communis	pycom04g19800
rosa_chinensis	RchiOBHm_Chr3g0451101
rosa_laevigata	RLG00000025690
rosa_multiflora	Rmu_ssc0000461.1_g000006
rosa_roxburghii	Rroxscaffold_6G00428570
rosa_rugosa	Rorug02G0631300
rosa_samantha	Rh3AG034300 Rh3BG034600 Rh3DG034300
rosa_wichuraiana	Rw3G002490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	303
AasI	GACNNNNNNGTC	2 cut(s)	254, 1829
Acc36I	ACCTGC	1 cut(s)	1790
AccB1I	GGYRCC	1 cut(s)	135
AccI	GTMKAC	1 cut(s)	1821
AccII	CGCG	1 cut(s)	949
AclWI	GGATC	7 cut(s)	55, 65, 68, 78, 442, 1356, 1934
AcoI	YGGCCR	1 cut(s)	252
AcsI	RAATTY	4 cut(s)	241, 793, 994, 1475
AcuI	CTGAAG	1 cut(s)	1644
AfaI	GTAC	3 cut(s)	356, 491, 1223
AfeI	AGCGCT	1 cut(s)	1134
AfiI	CCNNNNNNNGG	3 cut(s)	204, 930, 1857
AflIII	ACRYGT	2 cut(s)	822, 1218
AgeI	ACCGGT	1 cut(s)	486
AgsI	TTSAA	8 cut(s)	48, 145, 592, 1054, 1183, 1244, 1459, 1487
AjiI	CACGTC	1 cut(s)	565
AjnI	CCWGG	3 cut(s)	896, 1344, 1489
AloI	GAACNNNNNNTCC	2 cut(s)	735, 767
AluBI	AGCT	7 cut(s)	374, 638, 1415, 1658, 1669, 1746, 1918
AluI	AGCT	7 cut(s)	374, 638, 1415, 1658, 1669, 1746, 1918
Alw21I	GWGCWC	2 cut(s)	1503, 1630
Alw26I	GTCTC	2 cut(s)	1114, 1629
AlwI	GGATC	7 cut(s)	55, 65, 68, 78, 442, 1356, 1934
Aor51HI	AGCGCT	1 cut(s)	1134
AoxI	GGCC	6 cut(s)	252, 777, 1230, 1429, 1583, 1794
ApeKI	GCWGC	8 cut(s)	4, 27, 126, 911, 1115, 1118, 1727, 1922
ApoI	RAATTY	4 cut(s)	241, 793, 994, 1475
ArsI	GACNNNNNNTTYG	2 cut(s)	1460, 1492
AseI	ATTAAT	2 cut(s)	1001, 1211
AsiGI	ACCGGT	1 cut(s)	486
Asp700I	GAANNNNTTC	1 cut(s)	1049
AspLEI	GCGC	3 cut(s)	85, 949, 1135
AspS9I	GGNCC	3 cut(s)	171, 1138, 1519
AsuC2I	CCSGG	3 cut(s)	101, 1265, 1686
AsuHPI	GGTGA	3 cut(s)	89, 1054, 1402
AvaII	GGWCC	3 cut(s)	171, 1138, 1519
BamHI	GGATCC	2 cut(s)	60, 70
BanI	GGYRCC	1 cut(s)	135
BarI	GAAGNNNNNNTAC	2 cut(s)	1013, 1045
Bbv12I	GWGCWC	2 cut(s)	1503, 1630
BbvI	GCAGC	8 cut(s)	14, 16, 138, 898, 1102, 1130, 1714, 1909
BccI	CCATC	4 cut(s)	233, 529, 613, 1863
BceAI	ACGGC	1 cut(s)	239
BciT130I	CCWGG	3 cut(s)	898, 1346, 1491
BciVI	GTATCC	3 cut(s)	294, 571, 1694
BcnI	CCSGG	3 cut(s)	101, 1265, 1686
BcoDI	GTCTC	2 cut(s)	1114, 1629
BfaI	CTAG	3 cut(s)	698, 972, 1646
BfmI	CTRYAG	3 cut(s)	462, 912, 1116
BfoI	RGCGCY	1 cut(s)	1136
BfuAI	ACCTGC	1 cut(s)	1790
BfuI	GTATCC	3 cut(s)	294, 571, 1694
BglI	GCCNNNNNGGC	2 cut(s)	1428, 1582
BglII	AGATCT	1 cut(s)	979
Bme1390I	CCNGG	6 cut(s)	101, 898, 1265, 1346, 1491, 1686
Bme18I	GGWCC	3 cut(s)	171, 1138, 1519
BmgBI	CACGTC	1 cut(s)	565
BmgT120I	GGNCC	3 cut(s)	171, 1138, 1519
BmiI	GGNNCC	3 cut(s)	62, 72, 137
BmrFI	CCNGG	6 cut(s)	101, 898, 1265, 1346, 1491, 1686
BmrI	ACTGGG	1 cut(s)	76
BmsI	GCATC	7 cut(s)	428, 721, 859, 1164, 1353, 1563, 1870
BmuI	ACTGGG	1 cut(s)	76
BpmI	CTGGAG	1 cut(s)	1149
BpuEI	CTTGAG	2 cut(s)	695, 1658
BpuMI	CCSGG	3 cut(s)	101, 1265, 1686
BsaI	GGTCTC	1 cut(s)	1114
BsaJI	CCNNGG	7 cut(s)	906, 924, 1141, 1264, 1324, 1344, 1685
BsaWI	WCCGGW	1 cut(s)	486
Bsc4I	CCNNNNNNNGG	3 cut(s)	204, 930, 1857
Bse118I	RCCGGY	3 cut(s)	204, 486, 1576
Bse1I	ACTGG	2 cut(s)	71, 1132
Bse3DI	GCAATG	2 cut(s)	14, 1614
BseBI	CCWGG	3 cut(s)	898, 1346, 1491
BseDI	CCNNGG	7 cut(s)	906, 924, 1141, 1264, 1324, 1344, 1685
BseGI	GGATG	5 cut(s)	419, 688, 1179, 1444, 1861
BseLI	CCNNNNNNNGG	3 cut(s)	204, 930, 1857
BseMI	GCAATG	2 cut(s)	14, 1614
BseMII	CTCAG	1 cut(s)	1604
BseNI	ACTGG	2 cut(s)	71, 1132
BseRI	GAGGAG	1 cut(s)	70
BseXI	GCAGC	8 cut(s)	14, 16, 138, 898, 1102, 1130, 1714, 1909
BsgI	GTGCAG	1 cut(s)	145
Bsh1236I	CGCG	1 cut(s)	949
Bsh1285I	CGRYCG	1 cut(s)	1535
BshFI	GGCC	6 cut(s)	254, 779, 1232, 1431, 1585, 1796
BshNI	GGYRCC	1 cut(s)	135
BshTI	ACCGGT	1 cut(s)	486
BsiEI	CGRYCG	1 cut(s)	1535
BsiHKAI	GWGCWC	2 cut(s)	1503, 1630
BsiSI	CCGG	9 cut(s)	101, 205, 487, 575, 680, 1233, 1264, 1577, 1685
BslFI	GGGAC	2 cut(s)	569, 1281
BslI	CCNNNNNNNGG	3 cut(s)	204, 930, 1857
BsmAI	GTCTC	2 cut(s)	1114, 1629
BsmFI	GGGAC	2 cut(s)	569, 1281
BsmI	GAATGC	3 cut(s)	21, 1078, 1319
BsnI	GGCC	6 cut(s)	254, 779, 1232, 1431, 1585, 1796
Bso31I	GGTCTC	1 cut(s)	1114
Bsp1286I	GDGCHC	2 cut(s)	1503, 1630
Bsp1407I	TGTACA	1 cut(s)	354
Bsp19I	CCATGG	3 cut(s)	906, 1141, 1324
BspANI	GGCC	6 cut(s)	254, 779, 1232, 1431, 1585, 1796
BspCNI	CTCAG	1 cut(s)	1605
BspFNI	CGCG	1 cut(s)	949
BspHI	TCATGA	2 cut(s)	507, 597
BspLI	GGNNCC	3 cut(s)	62, 72, 137
BspMAI	CTGCAG	2 cut(s)	916, 1120
BspMI	ACCTGC	1 cut(s)	1790
BspPI	GGATC	7 cut(s)	55, 65, 68, 78, 442, 1356, 1934
BspT107I	GGYRCC	1 cut(s)	135
BspTNI	GGTCTC	1 cut(s)	1114
BsrDI	GCAATG	2 cut(s)	14, 1614
BsrFI	RCCGGY	3 cut(s)	204, 486, 1576
BsrGI	TGTACA	1 cut(s)	354
BsrI	ACTGG	2 cut(s)	71, 1132
BssAI	RCCGGY	3 cut(s)	204, 486, 1576
BssECI	CCNNGG	7 cut(s)	906, 924, 1141, 1264, 1324, 1344, 1685
BssT1I	CCWWGG	4 cut(s)	906, 924, 1141, 1324
Bst2UI	CCWGG	3 cut(s)	898, 1346, 1491
Bst4CI	ACNGT	7 cut(s)	79, 359, 552, 583, 648, 1088, 1291
Bst6I	CTCTTC	2 cut(s)	970, 1401
BstAPI	GCANNNNNTGC	2 cut(s)	27, 920
BstAUI	TGTACA	1 cut(s)	354
BstC8I	GCNNGC	5 cut(s)	206, 376, 543, 1545, 1920
BstDEI	CTNAG	1 cut(s)	1613
BstDSI	CCRYGG	3 cut(s)	906, 1141, 1324
BstF5I	GGATG	5 cut(s)	419, 688, 1179, 1444, 1861
BstFNI	CGCG	1 cut(s)	949
BstH2I	RGCGCY	1 cut(s)	1136
BstHHI	GCGC	3 cut(s)	85, 949, 1135
BstMAI	GTCTC	2 cut(s)	1114, 1629
BstMCI	CGRYCG	1 cut(s)	1535
BstMWI	GCNNNNNNNGC	9 cut(s)	13, 27, 920, 1013, 1372, 1428, 1582, 1625, 1655
BstNI	CCWGG	3 cut(s)	898, 1346, 1491
BstNSI	RCATGY	2 cut(s)	826, 1222
BstSCI	CCNGG	6 cut(s)	99, 896, 1263, 1344, 1489, 1684
BstSFI	CTRYAG	3 cut(s)	462, 912, 1116
BstUI	CGCG	1 cut(s)	949
BstV1I	GCAGC	8 cut(s)	14, 16, 138, 898, 1102, 1130, 1714, 1909
BstX2I	RGATCY	5 cut(s)	60, 70, 447, 979, 1348
BstYI	RGATCY	5 cut(s)	60, 70, 447, 979, 1348
BsuI	GTATCC	3 cut(s)	294, 571, 1694
BsuRI	GGCC	6 cut(s)	254, 779, 1232, 1431, 1585, 1796
BtgI	CCRYGG	3 cut(s)	906, 1141, 1324
BtrI	CACGTC	1 cut(s)	565
BtsCI	GGATG	5 cut(s)	419, 688, 1179, 1444, 1861
BtsI	GCAGTG	1 cut(s)	1901
BtsIMutI	CAGTG	2 cut(s)	64, 1901
BveI	ACCTGC	1 cut(s)	1790
Cac8I	GCNNGC	5 cut(s)	206, 376, 543, 1545, 1920
CciI	TCATGA	2 cut(s)	507, 597
CfoI	GCGC	3 cut(s)	85, 949, 1135
Cfr10I	RCCGGY	3 cut(s)	204, 486, 1576
Cfr13I	GGNCC	3 cut(s)	171, 1138, 1519
Csp6I	GTAC	3 cut(s)	355, 490, 1222
CspAI	ACCGGT	1 cut(s)	486
CviQI	GTAC	3 cut(s)	355, 490, 1222
DdeI	CTNAG	1 cut(s)	1613
DrdI	GACNNNNNNGTC	2 cut(s)	254, 1829
DseDI	GACNNNNNNGTC	2 cut(s)	254, 1829
EaeI	YGGCCR	1 cut(s)	252
Eam1104I	CTCTTC	2 cut(s)	970, 1401
EarI	CTCTTC	2 cut(s)	970, 1401
EciI	GGCGGA	2 cut(s)	211, 1713
Eco130I	CCWWGG	4 cut(s)	906, 924, 1141, 1324
Eco31I	GGTCTC	1 cut(s)	1114
Eco32I	GATATC	1 cut(s)	889
Eco47I	GGWCC	3 cut(s)	171, 1138, 1519
Eco47III	AGCGCT	1 cut(s)	1134
Eco57I	CTGAAG	1 cut(s)	1644
EcoO109I	RGGNCCY	1 cut(s)	171
EcoRI	GAATTC	1 cut(s)	994
EcoRII	CCWGG	3 cut(s)	896, 1344, 1489
EcoRV	GATATC	1 cut(s)	889
EcoT14I	CCWWGG	4 cut(s)	906, 924, 1141, 1324
EcoT22I	ATGCAT	2 cut(s)	760, 1158
ErhI	CCWWGG	4 cut(s)	906, 924, 1141, 1324
FaqI	GGGAC	2 cut(s)	569, 1281
FblI	GTMKAC	1 cut(s)	1821
FokI	GGATG	5 cut(s)	406, 695, 1186, 1451, 1848
FspBI	CTAG	3 cut(s)	698, 972, 1646
GlaI	GCGC	3 cut(s)	84, 948, 1134
GsuI	CTGGAG	1 cut(s)	1149
HaeII	RGCGCY	1 cut(s)	1136
HaeIII	GGCC	6 cut(s)	254, 779, 1232, 1431, 1585, 1796
HapII	CCGG	9 cut(s)	101, 205, 487, 575, 680, 1233, 1264, 1577, 1685
HhaI	GCGC	3 cut(s)	85, 949, 1135
Hin6I	GCGC	3 cut(s)	83, 947, 1133
HinP1I	GCGC	3 cut(s)	83, 947, 1133
HincII	GTYRAC	1 cut(s)	1822
HindII	GTYRAC	1 cut(s)	1822
HinfI	GANTC	3 cut(s)	1314, 1393, 1759
HpaII	CCGG	9 cut(s)	101, 205, 487, 575, 680, 1233, 1264, 1577, 1685
HphI	GGTGA	3 cut(s)	89, 1054, 1402
Hpy166II	GTNNAC	6 cut(s)	690, 719, 1519, 1816, 1822, 1834
Hpy188I	TCNGA	9 cut(s)	75, 189, 424, 634, 1360, 1383, 1753, 1866, 1934
Hpy188III	TCNNGA	9 cut(s)	331, 508, 521, 598, 674, 789, 1311, 1536, 1763
Hpy8I	GTNNAC	6 cut(s)	690, 719, 1519, 1816, 1822, 1834
Hpy99I	CGWCG	2 cut(s)	1823, 1826
HpyAV	CCTTC	9 cut(s)	410, 769, 877, 1015, 1039, 1261, 1436, 1533, 1786
HpyCH4III	ACNGT	7 cut(s)	79, 359, 552, 583, 648, 1088, 1291
HpyCH4IV	ACGT	3 cut(s)	564, 785, 1818
HpyF10VI	GCNNNNNNNGC	9 cut(s)	13, 27, 920, 1013, 1372, 1428, 1582, 1625, 1655
HpyF3I	CTNAG	1 cut(s)	1613
HpySE526I	ACGT	3 cut(s)	564, 785, 1818
HspAI	GCGC	3 cut(s)	83, 947, 1133
KroI	GCCGGC	1 cut(s)	204
KroNI	GCCGGC	1 cut(s)	206
LmnI	GCTCC	4 cut(s)	417, 1130, 1604, 1743
Lsp1109I	GCAGC	8 cut(s)	14, 16, 138, 898, 1102, 1130, 1714, 1909
LweI	GCATC	7 cut(s)	428, 721, 859, 1164, 1353, 1563, 1870
MaeI	CTAG	3 cut(s)	698, 972, 1646
MaeII	ACGT	3 cut(s)	564, 785, 1818
MaeIII	GTNAC	5 cut(s)	466, 480, 1285, 1328, 1390
MboII	GAAGA	8 cut(s)	523, 784, 987, 1158, 1388, 1801, 1873, 1886
MflI	RGATCY	5 cut(s)	60, 70, 447, 979, 1348
MhlI	GDGCHC	2 cut(s)	1503, 1630
MlyI	GAGTC	1 cut(s)	1387
MmeI	TCCRAC	2 cut(s)	96, 98
Mph1103I	ATGCAT	2 cut(s)	760, 1158
MroNI	GCCGGC	1 cut(s)	204
MroXI	GAANNNNTTC	1 cut(s)	1049
MseI	TTAA	5 cut(s)	179, 1001, 1101, 1211, 1449
MslI	CAYNNNNRTG	4 cut(s)	424, 506, 827, 1709
MspA1I	CMGCKG	2 cut(s)	1121, 1428
MspI	CCGG	9 cut(s)	101, 205, 487, 575, 680, 1233, 1264, 1577, 1685
MspR9I	CCNGG	6 cut(s)	101, 898, 1265, 1346, 1491, 1686
Mva1269I	GAATGC	3 cut(s)	21, 1078, 1319
MvaI	CCWGG	3 cut(s)	898, 1346, 1491
MvnI	CGCG	1 cut(s)	949
MwoI	GCNNNNNNNGC	9 cut(s)	13, 27, 920, 1013, 1372, 1428, 1582, 1625, 1655
NaeI	GCCGGC	1 cut(s)	206
NciI	CCSGG	3 cut(s)	101, 1265, 1686
NcoI	CCATGG	3 cut(s)	906, 1141, 1324
NgoMIV	GCCGGC	1 cut(s)	204
NlaIV	GGNNCC	3 cut(s)	62, 72, 137
NmeAIII	GCCGAG	1 cut(s)	715
NmuCI	GTSAC	2 cut(s)	480, 1390
NsiI	ATGCAT	2 cut(s)	760, 1158
NspI	RCATGY	2 cut(s)	826, 1222
PagI	TCATGA	2 cut(s)	507, 597
PciI	ACATGT	2 cut(s)	822, 1218
PctI	GAATGC	3 cut(s)	21, 1078, 1319
PdiI	GCCGGC	1 cut(s)	206
PdmI	GAANNNNTTC	1 cut(s)	1049
PfeI	GAWTC	2 cut(s)	1314, 1759
PfoI	TCCNGGA	1 cut(s)	896
PinAI	ACCGGT	1 cut(s)	486
Ple19I	CGATCG	1 cut(s)	1535
PleI	GAGTC	1 cut(s)	1387
PpsI	GAGTC	1 cut(s)	1387
PpuMI	RGGWCCY	1 cut(s)	171
PscI	ACATGT	2 cut(s)	822, 1218
PshBI	ATTAAT	2 cut(s)	1001, 1211
PsiI	TTATAA	1 cut(s)	303
Psp5II	RGGWCCY	1 cut(s)	171
Psp6I	CCWGG	3 cut(s)	896, 1344, 1489
PspGI	CCWGG	3 cut(s)	896, 1344, 1489
PspN4I	GGNNCC	3 cut(s)	62, 72, 137
PspPI	GGNCC	3 cut(s)	171, 1138, 1519
PspPPI	RGGWCCY	1 cut(s)	171
PstI	CTGCAG	2 cut(s)	916, 1120
PsuI	RGATCY	5 cut(s)	60, 70, 447, 979, 1348
PvuI	CGATCG	1 cut(s)	1535
RsaI	GTAC	3 cut(s)	356, 491, 1223
RsaNI	GTAC	3 cut(s)	355, 490, 1222
RseI	CAYNNNNRTG	4 cut(s)	424, 506, 827, 1709
SalI	GTCGAC	1 cut(s)	1820
SaqAI	TTAA	5 cut(s)	179, 1001, 1101, 1211, 1449
Sau96I	GGNCC	3 cut(s)	171, 1138, 1519
SchI	GAGTC	1 cut(s)	1387
ScrFI	CCNGG	6 cut(s)	101, 898, 1265, 1346, 1491, 1686
SduI	GDGCHC	2 cut(s)	1503, 1630
SfaNI	GCATC	7 cut(s)	428, 721, 859, 1164, 1353, 1563, 1870
SfcI	CTRYAG	3 cut(s)	462, 912, 1116
SgrDI	CGTCGACG	1 cut(s)	1820
SinI	GGWCC	3 cut(s)	171, 1138, 1519
SmiMI	CAYNNNNRTG	4 cut(s)	424, 506, 827, 1709
SmlI	CTYRAG	2 cut(s)	674, 1673
SmoI	CTYRAG	2 cut(s)	674, 1673
SspMI	CTAG	3 cut(s)	698, 972, 1646
StyD4I	CCNGG	6 cut(s)	99, 896, 1263, 1344, 1489, 1684
StyI	CCWWGG	4 cut(s)	906, 924, 1141, 1324
TaaI	ACNGT	7 cut(s)	79, 359, 552, 583, 648, 1088, 1291
TaiI	ACGT	3 cut(s)	567, 788, 1821
TatI	WGTACW	2 cut(s)	354, 1221
TauI	GCSGC	4 cut(s)	1431, 1799, 1850, 1861
TfiI	GAWTC	2 cut(s)	1314, 1759
Tru1I	TTAA	5 cut(s)	179, 1001, 1101, 1211, 1449
Tru9I	TTAA	5 cut(s)	179, 1001, 1101, 1211, 1449
TscAI	CASTG	2 cut(s)	71, 1901
TseFI	GTSAC	2 cut(s)	480, 1390
TseI	GCWGC	8 cut(s)	4, 27, 126, 911, 1115, 1118, 1727, 1922
Tsp45I	GTSAC	2 cut(s)	480, 1390
TspDTI	ATGAA	8 cut(s)	524, 669, 743, 987, 1243, 1455, 1916, 1928
TspGWI	ACGGA	2 cut(s)	270, 1839
TspRI	CASTG	2 cut(s)	71, 1901
VpaK11BI	GGWCC	3 cut(s)	171, 1138, 1519
VspI	ATTAAT	2 cut(s)	1001, 1211
XapI	RAATTY	4 cut(s)	241, 793, 994, 1475
XceI	RCATGY	2 cut(s)	826, 1222
XmiI	GTMKAC	1 cut(s)	1821
XmnI	GAANNNNTTC	1 cut(s)	1049
XspI	CTAG	3 cut(s)	698, 972, 1646
Zsp2I	ATGCAT	2 cut(s)	760, 1158

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.