MD04G1240600.v1.1

At4g13230-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0024412
Physical Location & Seq
Reverse (-)
31813470 .. 31815765
2296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1240600.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGCAAGCTTAACTAGTCTGTTTACCACCGTCCCCAAATCCGGCCTTGTCGGAGCATCCGTTGCAAGGAACGCCACTTCAACCCCTAGGATTTTTTTGGCTACAGCCCCTAGACGTATTCAAGCAAGTTCACAGCAAGATGCAGCTGCAGAAGTCAATGAAACTAGGAATGCTGCTGAAAATGTTTCGCAGACAGCCAAAGACATGGCCAGCAAGGTGTCTGCAACCGCACAGGATGTGTCTGAGAAAGCAAAGCAGACAGCACAAGATGCATGGAGCCAAGCTAAGGGCACCGCCCAGAAGGCGGCAGACACTGTGATGGGCAAGGCCGACGAATCCAAGGAATTCGTCAAAGAAAATGCTGAGCAGGTCAAGAAAAGCATGAATACCAAGAAAGAGACCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

14.28

Weight (kDa)

9.43

Isoelectric Point (pI)

29.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024412)

Species Orthologous Gene IDs
malus_domestica MD04G1240600.v1.1 MD12G1257600.v1.1
prunus_persica Prupe.6G358200_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 358
AccB1I GGYRCC 1 cut(s) 290
AciI CCGC 3 cut(s) 228, 294, 305
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 1 cut(s) 344
AfiI CCNNNNNNNGG 4 cut(s) 41, 66, 286, 304
AgsI TTSAA 2 cut(s) 81, 122
AhlI ACTAGT 1 cut(s) 14
AluBI AGCT 3 cut(s) 9, 146, 284
AluI AGCT 3 cut(s) 9, 146, 284
Alw26I GTCTC 1 cut(s) 392
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 3 cut(s) 43, 207, 327
ApeKI GCWGC 3 cut(s) 143, 146, 173
ApoI RAATTY 1 cut(s) 344
AspA2I CCTAGG 1 cut(s) 86
AvrII CCTAGG 1 cut(s) 86
BaeGI GKGCMC 1 cut(s) 293
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 1 cut(s) 290
BbvI GCAGC 3 cut(s) 133, 155, 160
BccI CCATC 1 cut(s) 313
BcoDI GTCTC 1 cut(s) 392
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 4 cut(s) 15, 87, 111, 165
BfmI CTRYAG 2 cut(s) 102, 147
BfuAI ACCTGC 1 cut(s) 358
BglI GCCNNNNNGGC 1 cut(s) 302
BisI GCNGC 4 cut(s) 144, 147, 174, 306
BlnI CCTAGG 1 cut(s) 86
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 4 cut(s) 145, 148, 175, 307
BmiI GGNNCC 2 cut(s) 278, 292
BmsI GCATC 3 cut(s) 65, 130, 259
Bpu10I CCTNAGC 1 cut(s) 285
Bpu1102I GCTNAGC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 392
BsaJI CCNNGG 2 cut(s) 86, 339
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 66, 286, 304
BseDI CCNNGG 2 cut(s) 86, 339
BseGI GGATG 2 cut(s) 56, 241
BseLI CCNNNNNNNGG 4 cut(s) 41, 66, 286, 304
BseMII CTCAG 2 cut(s) 234, 354
BseSI GKGCMC 1 cut(s) 293
BseXI GCAGC 3 cut(s) 133, 155, 160
BshFI GGCC 3 cut(s) 45, 209, 329
BshNI GGYRCC 1 cut(s) 290
BsiSI CCGG 1 cut(s) 42
BslFI GGGAC 1 cut(s) 17
BslI CCNNNNNNNGG 4 cut(s) 41, 66, 286, 304
BsmAI GTCTC 1 cut(s) 392
BsmFI GGGAC 1 cut(s) 17
BsmI GAATGC 1 cut(s) 175
BsnI GGCC 3 cut(s) 45, 209, 329
Bso31I GGTCTC 1 cut(s) 392
Bsp1286I GDGCHC 1 cut(s) 293
Bsp1720I GCTNAGC 1 cut(s) 363
BspACI CCGC 3 cut(s) 228, 294, 305
BspANI GGCC 3 cut(s) 45, 209, 329
BspCNI CTCAG 2 cut(s) 235, 355
BspLI GGNNCC 2 cut(s) 278, 292
BspMAI CTGCAG 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 358
BspT107I GGYRCC 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 392
BssECI CCNNGG 2 cut(s) 86, 339
BssT1I CCWWGG 2 cut(s) 86, 339
Bst4CI ACNGT 2 cut(s) 31, 316
BstAPI GCANNNNNTGC 2 cut(s) 62, 269
BstC8I GCNNGC 2 cut(s) 7, 211
BstDEI CTNAG 3 cut(s) 243, 285, 363
BstF5I GGATG 2 cut(s) 56, 241
BstMAI GTCTC 1 cut(s) 392
BstMWI GCNNNNNNNGC 4 cut(s) 62, 71, 269, 302
BstSFI CTRYAG 2 cut(s) 102, 147
BstSLI GKGCMC 1 cut(s) 293
BstV1I GCAGC 3 cut(s) 133, 155, 160
BstXI CCANNNNNNTGG 1 cut(s) 205
BsuRI GGCC 3 cut(s) 45, 209, 329
BtsCI GGATG 2 cut(s) 56, 241
BtsIMutI CAGTG 1 cut(s) 312
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 2 cut(s) 7, 211
CaiI CAGNNNCTG 1 cut(s) 314
CviAII CATG 3 cut(s) 205, 273, 382
DdeI CTNAG 3 cut(s) 243, 285, 363
EaeI YGGCCR 1 cut(s) 207
Eco130I CCWWGG 2 cut(s) 86, 339
Eco31I GGTCTC 1 cut(s) 392
EcoRI GAATTC 1 cut(s) 344
EcoT14I CCWWGG 2 cut(s) 86, 339
EcoT22I ATGCAT 1 cut(s) 274
ErhI CCWWGG 2 cut(s) 86, 339
FaeI CATG 3 cut(s) 208, 276, 385
FaiI YATR 5 cut(s) 206, 274, 383, 404, 406
FaqI GGGAC 1 cut(s) 17
FatI CATG 3 cut(s) 204, 272, 381
Fnu4HI GCNGC 4 cut(s) 144, 147, 174, 306
FokI GGATG 2 cut(s) 43, 248
Fsp4HI GCNGC 4 cut(s) 144, 147, 174, 306
FspBI CTAG 4 cut(s) 15, 87, 111, 165
GluI GCNGC 4 cut(s) 144, 147, 174, 306
HaeIII GGCC 3 cut(s) 45, 209, 329
HapII CCGG 1 cut(s) 42
Hin1II CATG 3 cut(s) 208, 276, 385
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 1 cut(s) 335
HpaII CCGG 1 cut(s) 42
Hpy166II GTNNAC 2 cut(s) 24, 131
Hpy188I TCNGA 2 cut(s) 53, 244
Hpy188III TCNNGA 1 cut(s) 373
Hpy8I GTNNAC 2 cut(s) 24, 131
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 2 cut(s) 31, 316
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 5 cut(s) 65, 143, 149, 224, 272
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 71, 269, 302
HpyF3I CTNAG 3 cut(s) 243, 285, 363
HpySE526I ACGT 1 cut(s) 115
Hsp92II CATG 3 cut(s) 208, 276, 385
LmnI GCTCC 2 cut(s) 53, 276
LpnPI CCDG 5 cut(s) 55, 218, 223, 311, 353
Lsp1109I GCAGC 3 cut(s) 133, 155, 160
LweI GCATC 3 cut(s) 65, 130, 259
MaeI CTAG 4 cut(s) 15, 87, 111, 165
MaeII ACGT 1 cut(s) 115
MhlI GDGCHC 1 cut(s) 293
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 1 cut(s) 344
MluNI TGGCCA 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 31
Mox20I TGGCCA 1 cut(s) 209
Mph1103I ATGCAT 1 cut(s) 274
MscI TGGCCA 1 cut(s) 209
MseI TTAA 1 cut(s) 11
MslI CAYNNNNRTG 1 cut(s) 317
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 175
MwoI GCNNNNNNNGC 4 cut(s) 62, 71, 269, 302
NlaIII CATG 3 cut(s) 208, 276, 385
NlaIV GGNNCC 2 cut(s) 278, 292
NsiI ATGCAT 1 cut(s) 274
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PctI GAATGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 335
PkrI GCNGC 4 cut(s) 145, 148, 175, 307
PspN4I GGNNCC 2 cut(s) 278, 292
PstI CTGCAG 1 cut(s) 151
PstNI CAGNNNCTG 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 317
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 4 cut(s) 144, 147, 174, 306
SduI GDGCHC 1 cut(s) 293
SetI ASST 6 cut(s) 11, 118, 148, 219, 286, 372
SfaNI GCATC 3 cut(s) 65, 130, 259
SfcI CTRYAG 2 cut(s) 102, 147
SmiMI CAYNNNNRTG 1 cut(s) 317
SpeI ACTAGT 1 cut(s) 14
Sse9I AATT 1 cut(s) 344
SsiI CCGC 3 cut(s) 228, 294, 305
SspMI CTAG 4 cut(s) 15, 87, 111, 165
StyI CCWWGG 2 cut(s) 86, 339
TaaI ACNGT 2 cut(s) 31, 316
TaiI ACGT 1 cut(s) 118
TasI AATT 1 cut(s) 344
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TscAI CASTG 1 cut(s) 319
TseI GCWGC 3 cut(s) 143, 146, 173
TspDTI ATGAA 2 cut(s) 174, 398
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 1 cut(s) 319
XapI RAATTY 1 cut(s) 344
XmaJI CCTAGG 1 cut(s) 86
XspI CTAG 4 cut(s) 15, 87, 111, 165
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.