MD12G1257600.v1.1

At4g13230-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0024412
Physical Location & Seq
Reverse (-)
32480260 .. 32481372
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1257600.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCAAGCTTAACTAGTCTGTTTACCACCCTCTCCAAATCCAGCCATGTCGGAGCAGCAATTGCAAGGAACGCCACTTCAACTCCTAGGATTTTTTTGGCTACAGCCCCTAGACGACGTATTCAAGCAAGTTCACAGCAAGATGCACCTGCAGTAGAGAATGAAGCTAGGAATGCTGCTGAAAATGTTTCGCAGACAACCAAAGACATGGCCGGCAAGGTGTCTGCAACCGCACAAGATGTGTCTGAGAAAGCAAAGCAGACAGCACAAGATGCTTGGAGCCAAGCCAAGGGCACCGCCCAGAAGGCGACAGACACCGTGATGGGCAAGGCCGAAGAATCCAAGGAGTTCGTCAAAGAAAATGCTGAGCAGGTCAAGAAAAGCATGAATACCAAGAAAGAGACCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

14.53

Weight (kDa)

9.6

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024412)

Species Orthologous Gene IDs
malus_domestica MD04G1240600.v1.1 MD12G1257600.v1.1
prunus_persica Prupe.6G358200_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 157
Acc36I ACCTGC 2 cut(s) 157, 361
AccB1I GGYRCC 1 cut(s) 293
AciI CCGC 2 cut(s) 231, 297
AcoI YGGCCR 1 cut(s) 210
AfiI CCNNNNNNNGG 1 cut(s) 289
AgsI TTSAA 2 cut(s) 81, 125
AhlI ACTAGT 1 cut(s) 14
AluBI AGCT 2 cut(s) 9, 167
AluI AGCT 2 cut(s) 9, 167
Alw26I GTCTC 1 cut(s) 395
AoxI GGCC 2 cut(s) 210, 330
ApeKI GCWGC 2 cut(s) 56, 176
AspA2I CCTAGG 1 cut(s) 86
AvrII CCTAGG 1 cut(s) 86
BaeGI GKGCMC 1 cut(s) 296
BanI GGYRCC 1 cut(s) 293
BbvI GCAGC 2 cut(s) 68, 163
BccI CCATC 1 cut(s) 316
BcoDI GTCTC 1 cut(s) 395
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 4 cut(s) 15, 87, 111, 168
BfmI CTRYAG 2 cut(s) 102, 150
BfuAI ACCTGC 2 cut(s) 157, 361
BglI GCCNNNNNGGC 1 cut(s) 305
BisI GCNGC 2 cut(s) 57, 177
BlnI CCTAGG 1 cut(s) 86
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 2 cut(s) 58, 178
BmiI GGNNCC 2 cut(s) 281, 295
BmsI GCATC 2 cut(s) 133, 262
Bpu1102I GCTNAGC 1 cut(s) 366
BsaI GGTCTC 1 cut(s) 395
BsaJI CCNNGG 3 cut(s) 86, 288, 342
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse118I RCCGGY 1 cut(s) 212
BseDI CCNNGG 3 cut(s) 86, 288, 342
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMII CTCAG 2 cut(s) 237, 357
BseSI GKGCMC 1 cut(s) 296
BseXI GCAGC 2 cut(s) 68, 163
BshFI GGCC 2 cut(s) 212, 332
BshNI GGYRCC 1 cut(s) 293
BsiSI CCGG 1 cut(s) 213
BslI CCNNNNNNNGG 1 cut(s) 289
BsmAI GTCTC 1 cut(s) 395
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 2 cut(s) 212, 332
Bso31I GGTCTC 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 296
Bsp1720I GCTNAGC 1 cut(s) 366
BspACI CCGC 2 cut(s) 231, 297
BspANI GGCC 2 cut(s) 212, 332
BspCNI CTCAG 2 cut(s) 238, 358
BspLI GGNNCC 2 cut(s) 281, 295
BspMAI CTGCAG 1 cut(s) 154
BspMI ACCTGC 2 cut(s) 157, 361
BspT107I GGYRCC 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 395
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 3 cut(s) 86, 288, 342
BssT1I CCWWGG 3 cut(s) 86, 288, 342
Bst4CI ACNGT 1 cut(s) 319
BstAPI GCANNNNNTGC 2 cut(s) 62, 272
BstC8I GCNNGC 2 cut(s) 7, 214
BstDEI CTNAG 2 cut(s) 246, 366
BstMAI GTCTC 1 cut(s) 395
BstMWI GCNNNNNNNGC 5 cut(s) 62, 71, 173, 272, 305
BstSFI CTRYAG 2 cut(s) 102, 150
BstSLI GKGCMC 1 cut(s) 296
BstV1I GCAGC 2 cut(s) 68, 163
BstXI CCANNNNNNTGG 1 cut(s) 208
BsuRI GGCC 2 cut(s) 212, 332
BveI ACCTGC 2 cut(s) 157, 361
Cac8I GCNNGC 2 cut(s) 7, 214
Cfr10I RCCGGY 1 cut(s) 212
CviAII CATG 3 cut(s) 47, 208, 385
CviJI RGCY 9 cut(s) 9, 45, 101, 107, 167, 212, 282, 287, 332
CviKI_1 RGCY 9 cut(s) 9, 45, 101, 107, 167, 212, 282, 287, 332
DdeI CTNAG 2 cut(s) 246, 366
EaeI YGGCCR 1 cut(s) 210
Eco130I CCWWGG 3 cut(s) 86, 288, 342
Eco31I GGTCTC 1 cut(s) 395
EcoT14I CCWWGG 3 cut(s) 86, 288, 342
ErhI CCWWGG 3 cut(s) 86, 288, 342
FaeI CATG 3 cut(s) 50, 211, 388
FaiI YATR 5 cut(s) 48, 209, 386, 407, 409
FatI CATG 3 cut(s) 46, 207, 384
Fnu4HI GCNGC 2 cut(s) 57, 177
Fsp4HI GCNGC 2 cut(s) 57, 177
FspBI CTAG 4 cut(s) 15, 87, 111, 168
GluI GCNGC 2 cut(s) 57, 177
HaeIII GGCC 2 cut(s) 212, 332
HapII CCGG 1 cut(s) 213
Hin1II CATG 3 cut(s) 50, 211, 388
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 1 cut(s) 338
HpaII CCGG 1 cut(s) 213
Hpy166II GTNNAC 2 cut(s) 24, 134
Hpy188I TCNGA 2 cut(s) 53, 247
Hpy188III TCNNGA 1 cut(s) 376
Hpy8I GTNNAC 2 cut(s) 24, 134
Hpy99I CGWCG 1 cut(s) 120
HpyAV CCTTC 1 cut(s) 298
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 4 cut(s) 65, 146, 152, 227
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 71, 173, 272, 305
HpyF3I CTNAG 2 cut(s) 246, 366
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 3 cut(s) 50, 211, 388
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
LmnI GCTCC 2 cut(s) 53, 279
LpnPI CCDG 5 cut(s) 55, 162, 226, 314, 356
Lsp1109I GCAGC 2 cut(s) 68, 163
LweI GCATC 2 cut(s) 133, 262
MaeI CTAG 4 cut(s) 15, 87, 111, 168
MaeII ACGT 1 cut(s) 118
MboII GAAGA 1 cut(s) 347
MfeI CAATTG 1 cut(s) 60
MhlI GDGCHC 1 cut(s) 296
MluCI AATT 1 cut(s) 60
MmeI TCCRAC 1 cut(s) 31
MnlI CCTC 1 cut(s) 41
MroNI GCCGGC 1 cut(s) 212
MseI TTAA 1 cut(s) 11
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 1 cut(s) 213
MunI CAATTG 1 cut(s) 60
Mva1269I GAATGC 1 cut(s) 178
MwoI GCNNNNNNNGC 5 cut(s) 62, 71, 173, 272, 305
NaeI GCCGGC 1 cut(s) 214
NgoMIV GCCGGC 1 cut(s) 212
NlaIII CATG 3 cut(s) 50, 211, 388
NlaIV GGNNCC 2 cut(s) 281, 295
PaqCI CACCTGC 1 cut(s) 157
PctI GAATGC 1 cut(s) 178
PdiI GCCGGC 1 cut(s) 214
PfeI GAWTC 1 cut(s) 338
PkrI GCNGC 2 cut(s) 58, 178
PspN4I GGNNCC 2 cut(s) 281, 295
PstI CTGCAG 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 320
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 2 cut(s) 57, 177
SduI GDGCHC 1 cut(s) 296
SetI ASST 6 cut(s) 11, 121, 151, 169, 222, 375
SfaNI GCATC 2 cut(s) 133, 262
SfcI CTRYAG 2 cut(s) 102, 150
SmiMI CAYNNNNRTG 1 cut(s) 320
SpeI ACTAGT 1 cut(s) 14
Sse9I AATT 1 cut(s) 60
SsiI CCGC 2 cut(s) 231, 297
SspMI CTAG 4 cut(s) 15, 87, 111, 168
StyI CCWWGG 3 cut(s) 86, 288, 342
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 1 cut(s) 121
TasI AATT 1 cut(s) 60
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TseI GCWGC 2 cut(s) 56, 176
TspDTI ATGAA 2 cut(s) 177, 401
XmaJI CCTAGG 1 cut(s) 86
XspI CTAG 4 cut(s) 15, 87, 111, 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.