MD05G1009100.v1.1
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024450
Physical Location & Seq
Forward (+)
1971763 .. 1974038
2276 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1009100.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGCTGGAACTGAAGCAACTACTCTACTGCTTTCTACTCAAAGCCAAAGGCCTTGTTTTTACTCGAGCAGTGTATCGAAAAATAAGAATTTGGTCAACTCCTTTTCAGGAGATGAAGGTGAATCATGTTATGTACTTCCAAATCCAGTCATTGACTACTCCATGCTCATCTCCGGTGATGTTCTAAAACGTACTAAAGCCATCAGTGACCTTAACGAAGCTTGCCTCAACTTTGGCTTCTTCACAGTGACGAATCATGGGATCCCGGACAGCCTGATGGGGAGTGTTATGAATTGGCTTTCCAATTTTTTTTATCAGAATGACGAGGAGAAGCGACGTTATGAGACAAATGATTCGAAGGATAAAATCAGATTCCGGTGGGGTGGCAGAACCCAAAGAGAGCTTCTCCACATGAGAGCACATCCCACCTTTCATTGCCCAACAAAGCCTGCCAATTCCACGGTTTTACAAGAGTATTGTGAGAAAATGCGAGAAATGGGAGTCCAATTACTTAGAGGGATTTCAAAATCCCTAGGGCTTGAAGAGGACTACATAGAGAAGAAAATGAACCTGGAATCTGGCTATAATGTTTTCGGACCAAATTACTATCCGGCGCTGTCGCATTCATCGGACGACAAGAATCAGATTGGCCAATTCCCTCATCGTGACCCTGGCTTACTTGTCCTCCTTGCTCAAGACGTTGATGGGGGACTTCAGATTGAGCACCAGAAAAAGTGGTTCAATGCAAACTTTCCTCCGAGTTCCATCTTTGTCATTGTTGCTGACCATATAGAGATTCTGACCAATGGGAAGTACAAGAGCTTGTTGCATCGGGTGGCTTTGACCAACGAAGTGGAAAGGAAGAGTTTACCCTTCTTTTTTGGACCATCATTGGAAGCAACTGTGAGGCCTGCACCTGAGTTTTTAGACGAGCACAACCCACCCTTTTATCGTGAAATGACGTATAAGGACTACTTGGAATCCAATAAGCACCATGTGATTGAAGCCAGAGCAAACTTGAATCAGATTCGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

346

Amino Acids

39.47

Weight (kDa)

6.32

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 49 - 127 2.4e-16 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 201 - 296 1.6e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024450)

Species Orthologous Gene IDs
malus_domestica MD05G1009100.v1.1
prunus_persica Prupe.8G011100_v2.0.a1
pyrus_communis pycom05g00470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 256, 269
AcoI YGGCCR 1 cut(s) 649
AcsI RAATTY 1 cut(s) 88
AcuI CTGAAG 2 cut(s) 33, 698
AdeI CACNNNGTG 1 cut(s) 997
AfaI GTAC 3 cut(s) 135, 193, 815
AgsI TTSAA 5 cut(s) 525, 542, 742, 1004, 1021
AjnI CCWGG 2 cut(s) 570, 670
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AluBI AGCT 3 cut(s) 221, 403, 822
AluI AGCT 3 cut(s) 221, 403, 822
Alw21I GWGCWC 3 cut(s) 421, 726, 936
Alw26I GTCTC 1 cut(s) 338
AlwI GGATC 2 cut(s) 256, 269
Ama87I CYCGRG 1 cut(s) 64
AoxI GGCC 3 cut(s) 50, 649, 908
ApoI RAATTY 1 cut(s) 88
ArsI GACNNNNNNTTYG 2 cut(s) 337, 369
AspA2I CCTAGG 1 cut(s) 532
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 2 cut(s) 596, 884
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 2 cut(s) 131, 188
AsuII TTCGAA 2 cut(s) 356, 1030
AvaI CYCGRG 1 cut(s) 64
AvaII GGWCC 2 cut(s) 596, 884
AvrII CCTAGG 1 cut(s) 532
BalI TGGCCA 1 cut(s) 651
BamHI GGATCC 1 cut(s) 261
Bbv12I GWGCWC 3 cut(s) 421, 726, 936
BccI CCATC 5 cut(s) 209, 271, 698, 773, 895
BciT130I CCWGG 2 cut(s) 572, 672
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 1 cut(s) 338
BfaI CTAG 1 cut(s) 533
BfoI RGCGCY 1 cut(s) 617
BlnI CCTAGG 1 cut(s) 532
Bme1390I CCNGG 3 cut(s) 266, 572, 672
Bme18I GGWCC 2 cut(s) 596, 884
BmeT110I CYCGRG 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 596, 884
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 3 cut(s) 266, 572, 672
BmsI GCATC 1 cut(s) 838
BplI GAGNNNNNCTC 2 cut(s) 390, 422
Bpu14I TTCGAA 2 cut(s) 356, 1030
BpuEI CTTGAG 1 cut(s) 678
BpuMI CCSGG 1 cut(s) 266
BsaBI GATNNNNATC 1 cut(s) 1031
BsaJI CCNNGG 3 cut(s) 459, 532, 670
BsaWI WCCGGW 2 cut(s) 173, 375
BsaXI ACNNNNNCTCC 2 cut(s) 669, 699
Bse1I ACTGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 433
Bse8I GATNNNNATC 1 cut(s) 1031
BseBI CCWGG 2 cut(s) 572, 672
BseDI CCNNGG 3 cut(s) 459, 532, 670
BseGI GGATG 1 cut(s) 421
BseJI GATNNNNATC 1 cut(s) 1031
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 1 cut(s) 909
BseNI ACTGG 1 cut(s) 146
BseRI GAGGAG 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 897
BshFI GGCC 3 cut(s) 52, 651, 910
BsiHKAI GWGCWC 3 cut(s) 421, 726, 936
BsiHKCI CYCGRG 1 cut(s) 64
BsiSI CCGG 4 cut(s) 174, 266, 376, 611
BslFI GGGAC 1 cut(s) 723
BsmAI GTCTC 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 723
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 3 cut(s) 52, 651, 910
BsoBI CYCGRG 1 cut(s) 64
Bsp119I TTCGAA 2 cut(s) 356, 1030
Bsp1286I GDGCHC 3 cut(s) 421, 726, 936
Bsp143I GATC 1 cut(s) 261
BspANI GGCC 3 cut(s) 52, 651, 910
BspCNI CTCAG 1 cut(s) 910
BspLI GGNNCC 1 cut(s) 263
BspPI GGATC 2 cut(s) 256, 269
BspT104I TTCGAA 2 cut(s) 356, 1030
BsrDI GCAATG 1 cut(s) 433
BsrI ACTGG 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 459, 532, 670
BssMI GATC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 532
Bst2UI CCWGG 2 cut(s) 572, 672
Bst4CI ACNGT 3 cut(s) 247, 463, 904
Bst6I CTCTTC 2 cut(s) 537, 857
BstBI TTCGAA 2 cut(s) 356, 1030
BstC8I GCNNGC 3 cut(s) 223, 450, 912
BstDEI CTNAG 2 cut(s) 512, 918
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 1 cut(s) 421
BstH2I RGCGCY 1 cut(s) 617
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 1 cut(s) 264
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 1 cut(s) 261
BstNI CCWGG 2 cut(s) 572, 672
BstSCI CCNGG 3 cut(s) 264, 570, 670
BstX2I RGATCY 1 cut(s) 261
BstXI CCANNNNNNTGG 1 cut(s) 853
BstYI RGATCY 1 cut(s) 261
BsuRI GGCC 3 cut(s) 52, 651, 910
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 1 cut(s) 421
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 3 cut(s) 76, 211, 252
Cac8I GCNNGC 3 cut(s) 223, 450, 912
CfoI GCGC 1 cut(s) 616
Cfr13I GGNCC 2 cut(s) 596, 884
Csp6I GTAC 3 cut(s) 134, 192, 814
CviAII CATG 5 cut(s) 126, 163, 257, 412, 995
CviQI GTAC 3 cut(s) 134, 192, 814
DdeI CTNAG 2 cut(s) 512, 918
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
DraIII CACNNNGTG 1 cut(s) 997
EaeI YGGCCR 1 cut(s) 649
Eam1104I CTCTTC 2 cut(s) 537, 857
EarI CTCTTC 2 cut(s) 537, 857
Eco130I CCWWGG 1 cut(s) 532
Eco147I AGGCCT 2 cut(s) 52, 910
Eco47I GGWCC 2 cut(s) 596, 884
Eco57I CTGAAG 2 cut(s) 33, 698
Eco88I CYCGRG 1 cut(s) 64
EcoRII CCWGG 2 cut(s) 570, 670
EcoT14I CCWWGG 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 532
FaeI CATG 5 cut(s) 129, 166, 260, 415, 998
FalI AAGNNNNNCTT 2 cut(s) 959, 991
FaqI GGGAC 1 cut(s) 723
FatI CATG 5 cut(s) 125, 162, 256, 411, 994
FokI GGATG 1 cut(s) 408
FspBI CTAG 1 cut(s) 533
GlaI GCGC 1 cut(s) 615
HaeII RGCGCY 1 cut(s) 617
HaeIII GGCC 3 cut(s) 52, 651, 910
HapII CCGG 4 cut(s) 174, 266, 376, 611
HhaI GCGC 1 cut(s) 616
Hin1II CATG 5 cut(s) 129, 166, 260, 415, 998
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 1 cut(s) 219
HpaII CCGG 4 cut(s) 174, 266, 376, 611
HphI GGTGA 2 cut(s) 131, 188
Hpy166II GTNNAC 2 cut(s) 97, 869
Hpy188III TCNNGA 4 cut(s) 108, 665, 695, 953
Hpy8I GTNNAC 2 cut(s) 97, 869
Hpy99I CGWCG 1 cut(s) 339
HpyAV CCTTC 3 cut(s) 110, 352, 883
HpyCH4III ACNGT 3 cut(s) 247, 463, 904
HpyCH4IV ACGT 4 cut(s) 190, 337, 699, 962
HpyCH4V TGCA 3 cut(s) 746, 829, 914
HpyF3I CTNAG 2 cut(s) 512, 918
HpySE526I ACGT 4 cut(s) 190, 337, 699, 962
Hsp92II CATG 5 cut(s) 129, 166, 260, 415, 998
HspAI GCGC 1 cut(s) 614
Kzo9I GATC 1 cut(s) 261
LweI GCATC 1 cut(s) 838
MaeI CTAG 1 cut(s) 533
MaeII ACGT 4 cut(s) 190, 337, 699, 962
MaeIII GTNAC 3 cut(s) 206, 247, 665
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 4 cut(s) 232, 554, 571, 874
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 3 cut(s) 421, 726, 936
MlsI TGGCCA 1 cut(s) 651
MluCI AATT 7 cut(s) 88, 292, 304, 454, 506, 601, 653
MluNI TGGCCA 1 cut(s) 651
MlyI GAGTC 1 cut(s) 510
MnlI CCTC 8 cut(s) 236, 319, 509, 538, 669, 695, 765, 900
Mox20I TGGCCA 1 cut(s) 651
MscI TGGCCA 1 cut(s) 651
MseI TTAA 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 651
MspI CCGG 4 cut(s) 174, 266, 376, 611
MspR9I CCNGG 3 cut(s) 266, 572, 672
Mva1269I GAATGC 1 cut(s) 622
MvaI CCWGG 2 cut(s) 572, 672
NciI CCSGG 1 cut(s) 266
NdeII GATC 1 cut(s) 261
NlaIII CATG 5 cut(s) 129, 166, 260, 415, 998
NlaIV GGNNCC 1 cut(s) 263
NmuCI GTSAC 3 cut(s) 206, 247, 665
NspV TTCGAA 2 cut(s) 356, 1030
PaeR7I CTCGAG 1 cut(s) 64
PceI AGGCCT 2 cut(s) 52, 910
PctI GAATGC 1 cut(s) 622
PfoI TCCNGGA 1 cut(s) 264
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
Psp6I CCWGG 2 cut(s) 570, 670
PspGI CCWGG 2 cut(s) 570, 670
PspN4I GGNNCC 1 cut(s) 263
PspPI GGNCC 2 cut(s) 596, 884
PspXI VCTCGAGB 1 cut(s) 64
PsuI RGATCY 1 cut(s) 261
RsaI GTAC 3 cut(s) 135, 193, 815
RsaNI GTAC 3 cut(s) 134, 192, 814
SaqAI TTAA 1 cut(s) 213
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 2 cut(s) 596, 884
SchI GAGTC 1 cut(s) 510
ScrFI CCNGG 3 cut(s) 266, 572, 672
SduI GDGCHC 3 cut(s) 421, 726, 936
SfaNI GCATC 1 cut(s) 838
Sfr274I CTCGAG 1 cut(s) 64
SfuI TTCGAA 2 cut(s) 356, 1030
SinI GGWCC 2 cut(s) 596, 884
SlaI CTCGAG 1 cut(s) 64
SmlI CTYRAG 2 cut(s) 64, 693
SmoI CTYRAG 2 cut(s) 64, 693
Sse9I AATT 7 cut(s) 88, 292, 304, 454, 506, 601, 653
SseBI AGGCCT 2 cut(s) 52, 910
SspMI CTAG 1 cut(s) 533
StuI AGGCCT 2 cut(s) 52, 910
StyD4I CCNGG 3 cut(s) 264, 570, 670
StyI CCWWGG 1 cut(s) 532
TaaI ACNGT 3 cut(s) 247, 463, 904
TaiI ACGT 4 cut(s) 193, 340, 702, 965
TaqI TCGA 4 cut(s) 65, 77, 356, 1030
TasI AATT 7 cut(s) 88, 292, 304, 454, 506, 601, 653
TatI WGTACW 2 cut(s) 133, 813
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 3 cut(s) 76, 211, 252
TseFI GTSAC 3 cut(s) 206, 247, 665
Tsp45I GTSAC 3 cut(s) 206, 247, 665
TspDTI ATGAA 5 cut(s) 129, 305, 422, 581, 615
TspRI CASTG 3 cut(s) 76, 211, 252
VpaK11BI GGWCC 2 cut(s) 596, 884
XapI RAATTY 1 cut(s) 88
XhoI CTCGAG 1 cut(s) 64
XmaJI CCTAGG 1 cut(s) 532
XspI CTAG 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.