pycom05g00470
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0024450
Physical Location & Seq
Forward (+)
591450 .. 594678
3229 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00470.2

Sequence Viewer

Length: 1398 bp
ATGGGACGAGACGGGACGGGACAGGACAGAACGGAATGGAGCGGGAGCAATGATGCCCTCGGATGGAAACAAGGAGGAAGAAGAAGGAGACGGAGAGGTTATAATTTTGTGTTCCACGGATGTGGAACGAGTCGTTCCAGGGGGGTGAGGTGGAATGAAAATTCACCCAAAACTCGTCCCGTGGAACAGCTCGTCCCACCCGTTTTAGGCGCACCAAACGTGGAACGGAACGTCTTATCCCACTATGTTCCATTCCGTCCCGTCCCACGTACCAAACGATACCTAAAGTACATGTTTATTTTGATAGTATCTTCCAATCGAACCGCAAAAATGGCTGGAACTGAAGCAACTACTCTACTGCTTTCTACTCAAAGCCAAAGGCCTTGTTTTTACTCGAGCAGTGTGTCGAAAAATAAGAATTTGGTCAACTCCTTTTCAGGATATGAAGGTGAATCATGTTATGTACTTCCAAATCCAGTCATTGACTACTCCATGCTCATCTCCGGTGATGTTCTAAAACGTACTAAAGCCATCAGTGACCTTAACGAAGCTTGCCTCAACTTTGGCTTCTTTACAGTGACGAATCATGGGATCCCGGACAGCCTGATGGGGAGTGTTATGAATTGGCTTTCCAAATTTTTTTATCAGAATGACGAGGAGAAGCGACGATATGAGACAAATGATTCGAAGGATAAAATCAGATTCCGGTGGGGTGGCAGAACCCAAAGAGAGCTTCTTCACATGAGAGCACATCCCACCTTTCATTGCCCAACAAAGCCTGCCAATTCCACGGTTTTACAAGAGTATTGTGAGAAAATGCGAGAAATGGGAGTCCAATTACTTAGAGGGATTTCAAAATCCCTAGGGCTTGAAGAGGACTACATAGAGAAGAAAATGAACCTGGAATCTGGCTATAATGTTTTCGGACCAAATTACTATCCGGCGCTGTCGCATTCATCGGACGACAAGAATCAGATTGGCCAATTCCCTCATCGTGACCCTGGCTTACTTGTCCTCCTTGCTCAAGACGTTGATGGGGGACTTCAGATTGAGCACCAGAAAAAGTGGTTCAATGCAAACTTTCCTCCGAGTTCCATCTTTGTCATTCTTGCTGACCATATAGAGGTTCATTTAAATTCTTCTCCACGAATAAACATTCTGACCAATGGGAAGTACAAGAGCTTGTTGCATCGGGTGGCTTTGACCAACGAAGTGGAAAGGAAGAGTTTACCCTTCTTTTTTGGACCATCATTGGAATCAACTGTGAGGCCTGCACCTGAGTTTGTAGACGAGCACAACCCACCCTTTTATCGTGAAATGACTTATAATGACTACTTGGAATCCAATAAGCACCATGTGATTGAAGCCAGAGCAAACTTGAATCAGATTCGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

466

Amino Acids

53.31

Weight (kDa)

9.34

Isoelectric Point (pI)

57.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024450)

Species Orthologous Gene IDs
malus_domestica MD05G1009100.v1.1
prunus_persica Prupe.8G011100_v2.0.a1
pyrus_communis pycom05g00470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 102, 1326
AccBSI CCGCTC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 1287
AciI CCGC 2 cut(s) 42, 324
AclWI GGATC 2 cut(s) 586, 599
AcoI YGGCCR 1 cut(s) 979
AcsI RAATTY 4 cut(s) 160, 418, 635, 1135
AcuI CTGAAG 2 cut(s) 363, 1028
AdeI CACNNNGTG 1 cut(s) 1357
AfaI GTAC 5 cut(s) 271, 290, 465, 523, 1175
AfiI CCNNNNNNNGG 3 cut(s) 63, 206, 1123
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 5 cut(s) 855, 872, 1072, 1364, 1381
AjnI CCWGG 3 cut(s) 137, 900, 1000
AjuI GAANNNNNNNTTGG 2 cut(s) 717, 749
AleI CACNNNNGTG 1 cut(s) 120
AloI GAACNNNNNNTCC 4 cut(s) 177, 209, 221, 253
AluBI AGCT 4 cut(s) 190, 551, 733, 1182
AluI AGCT 4 cut(s) 190, 551, 733, 1182
Alw21I GWGCWC 3 cut(s) 751, 1056, 1296
Alw26I GTCTC 3 cut(s) 3, 82, 668
AlwI GGATC 2 cut(s) 586, 599
Ama87I CYCGRG 1 cut(s) 394
AoxI GGCC 3 cut(s) 380, 979, 1268
ApoI RAATTY 4 cut(s) 160, 418, 635, 1135
ArsI GACNNNNNNTTYG 2 cut(s) 667, 699
AspA2I CCTAGG 1 cut(s) 862
AspLEI GCGC 2 cut(s) 212, 946
AspS9I GGNCC 2 cut(s) 926, 1244
AsuC2I CCSGG 1 cut(s) 596
AsuHPI GGTGA 4 cut(s) 156, 157, 461, 518
AsuII TTCGAA 2 cut(s) 686, 1390
AvaI CYCGRG 1 cut(s) 394
AvaII GGWCC 2 cut(s) 926, 1244
AvrII CCTAGG 1 cut(s) 862
BalI TGGCCA 1 cut(s) 981
BamHI GGATCC 1 cut(s) 591
Bbv12I GWGCWC 3 cut(s) 751, 1056, 1296
BccI CCATC 6 cut(s) 57, 539, 601, 1028, 1103, 1255
BciT130I CCWGG 3 cut(s) 139, 902, 1002
BcnI CCSGG 1 cut(s) 596
BcoDI GTCTC 3 cut(s) 3, 82, 668
BfaI CTAG 1 cut(s) 863
BfoI RGCGCY 1 cut(s) 947
BlnI CCTAGG 1 cut(s) 862
Bme1390I CCNGG 4 cut(s) 139, 596, 902, 1002
Bme18I GGWCC 2 cut(s) 926, 1244
BmeT110I CYCGRG 1 cut(s) 394
BmgT120I GGNCC 2 cut(s) 926, 1244
BmiI GGNNCC 1 cut(s) 593
BmrFI CCNGG 4 cut(s) 139, 596, 902, 1002
BmsI GCATC 2 cut(s) 43, 1198
Bpu14I TTCGAA 2 cut(s) 686, 1390
BpuEI CTTGAG 1 cut(s) 1008
BpuMI CCSGG 1 cut(s) 596
BsaAI YACGTR 1 cut(s) 269
BsaBI GATNNNNATC 1 cut(s) 1391
BsaJI CCNNGG 7 cut(s) 58, 115, 138, 180, 789, 862, 1000
BsaWI WCCGGW 2 cut(s) 503, 705
BsaXI ACNNNNNCTCC 2 cut(s) 999, 1029
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 206, 1123
Bse1I ACTGG 1 cut(s) 476
Bse3DI GCAATG 2 cut(s) 55, 763
Bse8I GATNNNNATC 1 cut(s) 1391
BseBI CCWGG 3 cut(s) 139, 902, 1002
BseDI CCNNGG 7 cut(s) 58, 115, 138, 180, 789, 862, 1000
BseGI GGATG 3 cut(s) 68, 125, 751
BseJI GATNNNNATC 1 cut(s) 1391
BseLI CCNNNNNNNGG 3 cut(s) 63, 206, 1123
BseMI GCAATG 2 cut(s) 55, 763
BseMII CTCAG 1 cut(s) 1269
BseNI ACTGG 1 cut(s) 476
BseRI GAGGAG 1 cut(s) 671
BsgI GTGCAG 1 cut(s) 1257
BshFI GGCC 3 cut(s) 382, 981, 1270
BsiHKAI GWGCWC 3 cut(s) 751, 1056, 1296
BsiHKCI CYCGRG 1 cut(s) 394
BsiSI CCGG 4 cut(s) 504, 596, 706, 941
BslFI GGGAC 8 cut(s) 18, 28, 33, 162, 179, 243, 248, 1053
BslI CCNNNNNNNGG 3 cut(s) 63, 206, 1123
BsmAI GTCTC 3 cut(s) 3, 82, 668
BsmBI CGTCTC 2 cut(s) 3, 82
BsmFI GGGAC 8 cut(s) 18, 28, 33, 162, 179, 243, 248, 1053
BsmI GAATGC 1 cut(s) 952
BsnI GGCC 3 cut(s) 382, 981, 1270
BsoBI CYCGRG 1 cut(s) 394
Bsp119I TTCGAA 2 cut(s) 686, 1390
Bsp1286I GDGCHC 3 cut(s) 751, 1056, 1296
Bsp143I GATC 1 cut(s) 591
BspACI CCGC 2 cut(s) 42, 324
BspANI GGCC 3 cut(s) 382, 981, 1270
BspCNI CTCAG 1 cut(s) 1270
BspLI GGNNCC 1 cut(s) 593
BspPI GGATC 2 cut(s) 586, 599
BspT104I TTCGAA 2 cut(s) 686, 1390
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 2 cut(s) 55, 763
BsrI ACTGG 1 cut(s) 476
BssECI CCNNGG 7 cut(s) 58, 115, 138, 180, 789, 862, 1000
BssMI GATC 1 cut(s) 591
BssT1I CCWWGG 1 cut(s) 862
Bst2UI CCWGG 3 cut(s) 139, 902, 1002
Bst4CI ACNGT 3 cut(s) 577, 793, 1264
Bst6I CTCTTC 2 cut(s) 867, 1217
BstBAI YACGTR 1 cut(s) 269
BstBI TTCGAA 2 cut(s) 686, 1390
BstC8I GCNNGC 3 cut(s) 553, 780, 1272
BstDEI CTNAG 2 cut(s) 842, 1278
BstDSI CCRYGG 3 cut(s) 115, 180, 789
BstF5I GGATG 3 cut(s) 68, 125, 751
BstH2I RGCGCY 1 cut(s) 947
BstHHI GCGC 2 cut(s) 212, 946
BstKTI GATC 1 cut(s) 594
BstMAI GTCTC 3 cut(s) 3, 82, 668
BstMBI GATC 1 cut(s) 591
BstMWI GCNNNNNNNGC 1 cut(s) 332
BstNI CCWGG 3 cut(s) 139, 902, 1002
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 4 cut(s) 137, 594, 900, 1000
BstX2I RGATCY 1 cut(s) 591
BstXI CCANNNNNNTGG 2 cut(s) 122, 1213
BstYI RGATCY 1 cut(s) 591
BsuRI GGCC 3 cut(s) 382, 981, 1270
BtgI CCRYGG 3 cut(s) 115, 180, 789
BtsCI GGATG 3 cut(s) 68, 125, 751
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 3 cut(s) 406, 541, 582
Cac8I GCNNGC 3 cut(s) 553, 780, 1272
CfoI GCGC 2 cut(s) 212, 946
Cfr13I GGNCC 2 cut(s) 926, 1244
Csp6I GTAC 5 cut(s) 270, 289, 464, 522, 1174
CviAII CATG 6 cut(s) 292, 456, 493, 587, 742, 1355
CviQI GTAC 5 cut(s) 270, 289, 464, 522, 1174
DdeI CTNAG 2 cut(s) 842, 1278
DpnI GATC 1 cut(s) 593
DpnII GATC 1 cut(s) 591
DraI TTTAAA 1 cut(s) 1134
DraIII CACNNNGTG 1 cut(s) 1357
EaeI YGGCCR 1 cut(s) 979
Eam1104I CTCTTC 2 cut(s) 867, 1217
EarI CTCTTC 2 cut(s) 867, 1217
Eco130I CCWWGG 1 cut(s) 862
Eco147I AGGCCT 2 cut(s) 382, 1270
Eco47I GGWCC 2 cut(s) 926, 1244
Eco57I CTGAAG 2 cut(s) 363, 1028
Eco88I CYCGRG 1 cut(s) 394
EcoRII CCWGG 3 cut(s) 137, 900, 1000
EcoT14I CCWWGG 1 cut(s) 862
ErhI CCWWGG 1 cut(s) 862
Esp3I CGTCTC 2 cut(s) 3, 82
FaeI CATG 6 cut(s) 295, 459, 496, 590, 745, 1358
FaqI GGGAC 8 cut(s) 18, 28, 33, 162, 179, 243, 248, 1053
FatI CATG 6 cut(s) 291, 455, 492, 586, 741, 1354
FauI CCCGC 1 cut(s) 35
FblI GTMKAC 1 cut(s) 1287
FokI GGATG 3 cut(s) 75, 132, 738
FspBI CTAG 1 cut(s) 863
GlaI GCGC 2 cut(s) 211, 945
HaeII RGCGCY 1 cut(s) 947
HaeIII GGCC 3 cut(s) 382, 981, 1270
HapII CCGG 4 cut(s) 504, 596, 706, 941
HhaI GCGC 2 cut(s) 212, 946
Hin1II CATG 6 cut(s) 295, 459, 496, 590, 745, 1358
Hin6I GCGC 2 cut(s) 210, 944
HinP1I GCGC 2 cut(s) 210, 944
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 549
HpaII CCGG 4 cut(s) 504, 596, 706, 941
HphI GGTGA 4 cut(s) 156, 157, 461, 518
Hpy166II GTNNAC 3 cut(s) 427, 1229, 1288
Hpy188III TCNNGA 4 cut(s) 438, 995, 1025, 1313
Hpy8I GTNNAC 3 cut(s) 427, 1229, 1288
Hpy99I CGWCG 1 cut(s) 669
HpyAV CCTTC 4 cut(s) 78, 440, 682, 1243
HpyCH4III ACNGT 3 cut(s) 577, 793, 1264
HpyCH4IV ACGT 5 cut(s) 219, 231, 268, 520, 1029
HpyCH4V TGCA 3 cut(s) 1076, 1189, 1274
HpyF10VI GCNNNNNNNGC 1 cut(s) 332
HpyF3I CTNAG 2 cut(s) 842, 1278
HpySE526I ACGT 5 cut(s) 219, 231, 268, 520, 1029
Hsp92II CATG 6 cut(s) 295, 459, 496, 590, 745, 1358
HspAI GCGC 2 cut(s) 210, 944
Kzo9I GATC 1 cut(s) 591
LmnI GCTCC 2 cut(s) 39, 45
LweI GCATC 2 cut(s) 43, 1198
MaeI CTAG 1 cut(s) 863
MaeII ACGT 5 cut(s) 219, 231, 268, 520, 1029
MaeIII GTNAC 3 cut(s) 536, 577, 995
MalI GATC 1 cut(s) 593
MbiI CCGCTC 1 cut(s) 42
MboI GATC 1 cut(s) 591
MboII GAAGA 8 cut(s) 90, 93, 303, 728, 884, 901, 1131, 1234
MflI RGATCY 1 cut(s) 591
MhlI GDGCHC 3 cut(s) 751, 1056, 1296
MlsI TGGCCA 1 cut(s) 981
MluNI TGGCCA 1 cut(s) 981
MlyI GAGTC 2 cut(s) 139, 840
Mox20I TGGCCA 1 cut(s) 981
MscI TGGCCA 1 cut(s) 981
MseI TTAA 2 cut(s) 543, 1133
MslI CAYNNNNRTG 1 cut(s) 120
Msp20I TGGCCA 1 cut(s) 981
MspI CCGG 4 cut(s) 504, 596, 706, 941
MspR9I CCNGG 4 cut(s) 139, 596, 902, 1002
Mva1269I GAATGC 1 cut(s) 952
MvaI CCWGG 3 cut(s) 139, 902, 1002
MwoI GCNNNNNNNGC 1 cut(s) 332
NciI CCSGG 1 cut(s) 596
NdeII GATC 1 cut(s) 591
NlaIII CATG 6 cut(s) 295, 459, 496, 590, 745, 1358
NlaIV GGNNCC 1 cut(s) 593
NmuCI GTSAC 3 cut(s) 536, 577, 995
NspI RCATGY 1 cut(s) 295
NspV TTCGAA 2 cut(s) 686, 1390
OliI CACNNNNGTG 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 394
PceI AGGCCT 2 cut(s) 382, 1270
PciI ACATGT 1 cut(s) 291
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 274
PctI GAATGC 1 cut(s) 952
PfoI TCCNGGA 1 cut(s) 594
PleI GAGTC 2 cut(s) 138, 839
PpsI GAGTC 2 cut(s) 138, 839
Ppu21I YACGTR 1 cut(s) 269
PscI ACATGT 1 cut(s) 291
PsiI TTATAA 2 cut(s) 102, 1326
Psp6I CCWGG 3 cut(s) 137, 900, 1000
PspGI CCWGG 3 cut(s) 137, 900, 1000
PspN4I GGNNCC 1 cut(s) 593
PspPI GGNCC 2 cut(s) 926, 1244
PspXI VCTCGAGB 1 cut(s) 394
PsuI RGATCY 1 cut(s) 591
RsaI GTAC 5 cut(s) 271, 290, 465, 523, 1175
RsaNI GTAC 5 cut(s) 270, 289, 464, 522, 1174
RseI CAYNNNNRTG 1 cut(s) 120
SaqAI TTAA 2 cut(s) 543, 1133
Sau3AI GATC 1 cut(s) 591
Sau96I GGNCC 2 cut(s) 926, 1244
SchI GAGTC 2 cut(s) 139, 840
ScrFI CCNGG 4 cut(s) 139, 596, 902, 1002
SduI GDGCHC 3 cut(s) 751, 1056, 1296
SfaNI GCATC 2 cut(s) 43, 1198
Sfr274I CTCGAG 1 cut(s) 394
SfuI TTCGAA 2 cut(s) 686, 1390
SinI GGWCC 2 cut(s) 926, 1244
SlaI CTCGAG 1 cut(s) 394
SmiI ATTTAAAT 1 cut(s) 1134
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 2 cut(s) 394, 1023
SmoI CTYRAG 2 cut(s) 394, 1023
SseBI AGGCCT 2 cut(s) 382, 1270
SsiI CCGC 2 cut(s) 42, 324
SspMI CTAG 1 cut(s) 863
StuI AGGCCT 2 cut(s) 382, 1270
StyD4I CCNGG 4 cut(s) 137, 594, 900, 1000
StyI CCWWGG 1 cut(s) 862
SwaI ATTTAAAT 1 cut(s) 1134
TaaI ACNGT 3 cut(s) 577, 793, 1264
TaiI ACGT 5 cut(s) 222, 234, 271, 523, 1032
TaqI TCGA 5 cut(s) 319, 395, 407, 686, 1390
TatI WGTACW 3 cut(s) 288, 463, 1173
Tru1I TTAA 2 cut(s) 543, 1133
Tru9I TTAA 2 cut(s) 543, 1133
TscAI CASTG 3 cut(s) 406, 541, 582
TseFI GTSAC 3 cut(s) 536, 577, 995
Tsp45I GTSAC 3 cut(s) 536, 577, 995
TspDTI ATGAA 7 cut(s) 171, 459, 635, 752, 911, 945, 1118
TspGWI ACGGA 5 cut(s) 47, 106, 132, 241, 245
TspRI CASTG 3 cut(s) 406, 541, 582
VpaK11BI GGWCC 2 cut(s) 926, 1244
XapI RAATTY 4 cut(s) 160, 418, 635, 1135
XceI RCATGY 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 394
XmaJI CCTAGG 1 cut(s) 862
XmiI GTMKAC 1 cut(s) 1287
XspI CTAG 1 cut(s) 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.