Prupe.8G011100_v2.0.a1
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0024450
Physical Location & Seq
Forward (+)
912590 .. 915381
2792 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G011100.1

Sequence Viewer

Length: 1035 bp
ATGTCTGGAACTCGAGCAACTCCACTACTGCTTACTCAAAGTCAAAACCCTTGTTTTCAGTCCTCACAAAGTGTTTCAAGTACCAACGGCAGCATAAACTGCATTTCAGGACGTGACCATGAATCATGTCATGTTCCAAATCCAATCATTGACTACTCCATGCTCAACTCCAATGATTGTCTCAAAAGGTCTCAAGCTATCAATGACCTCAATCAAGCTTGCCTCAACTATGGCTTCTTCGCAGTGACAAATCATGGGATCCCAGATAGCTTGATTGGGAGTGTTATGAATTGGCTTTCCAAATTTTTTAATCAAAGTGATGAGGAGAAGCGAAGGTATGATACGAATGATCCGACGGATAGGATCAGATTCAGGTGGGGCGGCAGAACCCAAAGAGAGCTTCTCCACATGAGGGCACATCCCACCTTCCATTGCCCAACAAAGCCTGCCGATTCCATGGTTTTAACAGAGTATTGTGAGAGAATGCGAGAGATGGGAATGCAATTACTTAGAGGGATTTCAAAATCTCTAGGGCTTGAAGAATGCTACATAGAAAACAAAATGAAGCTGGAATCGGGCTACAACGTTTTTGGACCAAATTACTATCCAGCACTGTCGCAGTCCTCGGATGATAAGAATCAGATTGGCCAATTCCCTCATCGCGACCCTGGTTTACTTGTTCTCCTTGCACAAAACGTGGGTGGGGGGCTTCAGATAGAACATCAGAAGAAGTGGCTCAATGCAGATTTTCCTCCTAGTTCCATTTTTGTCATTGTTGCCGACCATATAGAGATTCTTACCAATGGAAAATACAAGAGCTTGTTGCATCGGGTGGCCTTAAACAGCGAAGTGGAAAGGATGAGTTTACCCTTCTTTTTTGGGCCATCGTTGGACGTAACTGTGAAGCCTGAACCAGAGTTTGTGGACGACCACAACCCACCCTCTTATCGTCAAATGACTTACAAAGCATACTTGGAATCCAATGACCACCATGTGATTGAAGCGAGAGCAAACTTGAATCAGATTCGACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

345

Amino Acids

39.12

Weight (kDa)

6.35

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024450)

Species Orthologous Gene IDs
malus_domestica MD05G1009100.v1.1
prunus_persica Prupe.8G011100_v2.0.a1
pyrus_communis pycom05g00470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 663
AciI CCGC 1 cut(s) 381
AclI AACGTT 1 cut(s) 585
AclWI GGATC 4 cut(s) 253, 266, 344, 371
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 1 cut(s) 302
AcuI CTGAAG 1 cut(s) 695
AdeI CACNNNGTG 2 cut(s) 71, 994
AfaI GTAC 1 cut(s) 82
AfiI CCNNNNNNNGG 1 cut(s) 412
AgsI TTSAA 5 cut(s) 78, 522, 539, 1001, 1018
AjiI CACGTC 1 cut(s) 113
AjnI CCWGG 1 cut(s) 667
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AluBI AGCT 6 cut(s) 197, 218, 270, 400, 568, 819
AluI AGCT 6 cut(s) 197, 218, 270, 400, 568, 819
Alw26I GTCTC 2 cut(s) 185, 195
AlwI GGATC 4 cut(s) 253, 266, 344, 371
Ama87I CYCGRG 1 cut(s) 12
AoxI GGCC 3 cut(s) 646, 834, 881
ApeKI GCWGC 1 cut(s) 90
ApoI RAATTY 1 cut(s) 302
AspS9I GGNCC 2 cut(s) 593, 881
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 1 cut(s) 593
BaeGI GKGCMC 1 cut(s) 418
BalI TGGCCA 1 cut(s) 648
BamHI GGATCC 1 cut(s) 258
BarI GAAGNNNNNNTAC 2 cut(s) 325, 357
BbvI GCAGC 1 cut(s) 102
BccI CCATC 2 cut(s) 487, 892
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 1 cut(s) 669
BcoDI GTCTC 2 cut(s) 185, 195
BfaI CTAG 2 cut(s) 530, 756
BisI GCNGC 2 cut(s) 91, 382
BlsI GCNGC 2 cut(s) 92, 383
Bme1390I CCNGG 1 cut(s) 669
Bme18I GGWCC 1 cut(s) 593
BmeT110I CYCGRG 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 593, 881
BmiI GGNNCC 1 cut(s) 260
BmrFI CCNGG 1 cut(s) 669
BmsI GCATC 1 cut(s) 835
BplI GAGNNNNNCTC 2 cut(s) 387, 419
BpuEI CTTGAG 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 636
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 3 cut(s) 456, 624, 667
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse3DI GCAATG 1 cut(s) 430
Bse8I GATNNNNATC 1 cut(s) 636
BseBI CCWGG 1 cut(s) 669
BseDI CCNNGG 3 cut(s) 456, 624, 667
BseGI GGATG 3 cut(s) 418, 634, 864
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMI GCAATG 1 cut(s) 430
BseRI GAGGAG 1 cut(s) 338
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 663
BshFI GGCC 3 cut(s) 648, 836, 883
BsiHKCI CYCGRG 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 412
BsmAI GTCTC 2 cut(s) 185, 195
BsmI GAATGC 3 cut(s) 489, 504, 548
BsnI GGCC 3 cut(s) 648, 836, 883
Bso31I GGTCTC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 3 cut(s) 258, 349, 363
Bsp19I CCATGG 1 cut(s) 456
Bsp68I TCGCGA 1 cut(s) 663
BspACI CCGC 1 cut(s) 381
BspANI GGCC 3 cut(s) 648, 836, 883
BspFNI CGCG 1 cut(s) 663
BspLI GGNNCC 1 cut(s) 260
BspPI GGATC 4 cut(s) 253, 266, 344, 371
BspTNI GGTCTC 1 cut(s) 195
BsrDI GCAATG 1 cut(s) 430
BssECI CCNNGG 3 cut(s) 456, 624, 667
BssMI GATC 3 cut(s) 258, 349, 363
BssT1I CCWWGG 1 cut(s) 456
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 2 cut(s) 615, 901
BstAPI GCANNNNNTGC 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 220, 447
BstDEI CTNAG 1 cut(s) 509
BstDSI CCRYGG 1 cut(s) 456
BstF5I GGATG 3 cut(s) 418, 634, 864
BstFNI CGCG 1 cut(s) 663
BstKTI GATC 3 cut(s) 261, 352, 366
BstMAI GTCTC 2 cut(s) 185, 195
BstMBI GATC 3 cut(s) 258, 349, 363
BstMWI GCNNNNNNNGC 1 cut(s) 99
BstNI CCWGG 1 cut(s) 669
BstSCI CCNGG 1 cut(s) 667
BstSLI GKGCMC 1 cut(s) 418
BstUI CGCG 1 cut(s) 663
BstV1I GCAGC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
BsuRI GGCC 3 cut(s) 648, 836, 883
BtgI CCRYGG 1 cut(s) 456
BtgZI GCGATG 1 cut(s) 644
BtrI CACGTC 1 cut(s) 113
BtsCI GGATG 3 cut(s) 418, 634, 864
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 2 cut(s) 249, 611
BtuMI TCGCGA 1 cut(s) 663
Cac8I GCNNGC 2 cut(s) 220, 447
Cfr13I GGNCC 2 cut(s) 593, 881
Csp6I GTAC 1 cut(s) 81
CviAII CATG 8 cut(s) 119, 126, 131, 160, 254, 409, 457, 992
CviQI GTAC 1 cut(s) 81
DdeI CTNAG 1 cut(s) 509
DpnI GATC 3 cut(s) 260, 351, 365
DpnII GATC 3 cut(s) 258, 349, 363
DraIII CACNNNGTG 2 cut(s) 71, 994
EaeI YGGCCR 1 cut(s) 646
Eco130I CCWWGG 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 593
Eco57I CTGAAG 1 cut(s) 695
Eco88I CYCGRG 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 456
FaeI CATG 8 cut(s) 122, 129, 134, 163, 257, 412, 460, 995
FatI CATG 8 cut(s) 118, 125, 130, 159, 253, 408, 456, 991
Fnu4HI GCNGC 2 cut(s) 91, 382
FokI GGATG 3 cut(s) 405, 641, 871
Fsp4HI GCNGC 2 cut(s) 91, 382
FspBI CTAG 2 cut(s) 530, 756
GluI GCNGC 2 cut(s) 91, 382
HaeIII GGCC 3 cut(s) 648, 836, 883
Hin1II CATG 8 cut(s) 122, 129, 134, 163, 257, 412, 460, 995
HindIII AAGCTT 1 cut(s) 216
Hpy166II GTNNAC 3 cut(s) 674, 866, 925
Hpy188I TCNGA 8 cut(s) 354, 368, 628, 642, 714, 726, 1023, 1034
Hpy188III TCNNGA 3 cut(s) 6, 108, 662
Hpy8I GTNNAC 3 cut(s) 674, 866, 925
Hpy99I CGWCG 1 cut(s) 358
HpyAV CCTTC 3 cut(s) 327, 436, 880
HpyCH4III ACNGT 2 cut(s) 615, 901
HpyCH4IV ACGT 4 cut(s) 112, 585, 696, 894
HpyCH4V TGCA 5 cut(s) 102, 502, 689, 743, 826
HpyF10VI GCNNNNNNNGC 1 cut(s) 99
HpyF3I CTNAG 1 cut(s) 509
HpySE526I ACGT 4 cut(s) 112, 585, 696, 894
Hsp92II CATG 8 cut(s) 122, 129, 134, 163, 257, 412, 460, 995
Kzo9I GATC 3 cut(s) 258, 349, 363
Lsp1109I GCAGC 1 cut(s) 102
LweI GCATC 1 cut(s) 835
MaeI CTAG 2 cut(s) 530, 756
MaeII ACGT 4 cut(s) 112, 585, 696, 894
MaeIII GTNAC 3 cut(s) 113, 244, 895
MalI GATC 3 cut(s) 260, 351, 365
MboI GATC 3 cut(s) 258, 349, 363
MboII GAAGA 3 cut(s) 229, 551, 739
MflI RGATCY 1 cut(s) 258
MhlI GDGCHC 1 cut(s) 418
MlsI TGGCCA 1 cut(s) 648
MluCI AATT 5 cut(s) 289, 302, 503, 598, 650
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 1 cut(s) 1023
MmeI TCCRAC 2 cut(s) 377, 870
Mox20I TGGCCA 1 cut(s) 648
MscI TGGCCA 1 cut(s) 648
MseI TTAA 3 cut(s) 309, 464, 839
Msp20I TGGCCA 1 cut(s) 648
MspR9I CCNGG 1 cut(s) 669
Mva1269I GAATGC 3 cut(s) 489, 504, 548
MvaI CCWGG 1 cut(s) 669
MvnI CGCG 1 cut(s) 663
MwoI GCNNNNNNNGC 1 cut(s) 99
NcoI CCATGG 1 cut(s) 456
NdeII GATC 3 cut(s) 258, 349, 363
NlaIII CATG 8 cut(s) 122, 129, 134, 163, 257, 412, 460, 995
NlaIV GGNNCC 1 cut(s) 260
NmuCI GTSAC 2 cut(s) 113, 244
NruI TCGCGA 1 cut(s) 663
PaeR7I CTCGAG 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 350
PctI GAATGC 3 cut(s) 489, 504, 548
PfeI GAWTC 9 cut(s) 122, 369, 452, 572, 637, 793, 977, 1018, 1024
PkrI GCNGC 2 cut(s) 92, 383
PleI GAGTC 1 cut(s) 1023
PpsI GAGTC 1 cut(s) 1023
Psp1406I AACGTT 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 1 cut(s) 260
PspPI GGNCC 2 cut(s) 593, 881
PspXI VCTCGAGB 1 cut(s) 12
PsuI RGATCY 1 cut(s) 258
RruI TCGCGA 1 cut(s) 663
RsaI GTAC 1 cut(s) 82
RsaNI GTAC 1 cut(s) 81
SaqAI TTAA 3 cut(s) 309, 464, 839
SatI GCNGC 2 cut(s) 91, 382
Sau3AI GATC 3 cut(s) 258, 349, 363
Sau96I GGNCC 2 cut(s) 593, 881
SchI GAGTC 1 cut(s) 1023
ScrFI CCNGG 1 cut(s) 669
SduI GDGCHC 1 cut(s) 418
SfaNI GCATC 1 cut(s) 835
Sfr274I CTCGAG 1 cut(s) 12
SinI GGWCC 1 cut(s) 593
SlaI CTCGAG 1 cut(s) 12
SmlI CTYRAG 2 cut(s) 12, 192
SmoI CTYRAG 2 cut(s) 12, 192
Sse9I AATT 5 cut(s) 289, 302, 503, 598, 650
SsiI CCGC 1 cut(s) 381
SspMI CTAG 2 cut(s) 530, 756
StyD4I CCNGG 1 cut(s) 667
StyI CCWWGG 1 cut(s) 456
TaaI ACNGT 2 cut(s) 615, 901
TaiI ACGT 4 cut(s) 115, 588, 699, 897
TaqI TCGA 2 cut(s) 13, 1027
TasI AATT 5 cut(s) 289, 302, 503, 598, 650
TauI GCSGC 1 cut(s) 384
TfiI GAWTC 9 cut(s) 122, 369, 452, 572, 637, 793, 977, 1018, 1024
Tru1I TTAA 3 cut(s) 309, 464, 839
Tru9I TTAA 3 cut(s) 309, 464, 839
TscAI CASTG 2 cut(s) 249, 618
TseFI GTSAC 2 cut(s) 113, 244
TseI GCWGC 1 cut(s) 90
Tsp45I GTSAC 2 cut(s) 113, 244
TspDTI ATGAA 3 cut(s) 135, 302, 578
TspGWI ACGGA 1 cut(s) 371
TspRI CASTG 2 cut(s) 249, 618
VpaK11BI GGWCC 1 cut(s) 593
XapI RAATTY 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 12
XspI CTAG 2 cut(s) 530, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.