Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0015124

Physical Location & Seq

Genomic Coordinates Forward (+)

2386057 .. 2391230

Gene Structure 5174 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1011800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGCAACTGTGGTTGCCATGCCCAAGATCCGGATGAAGATTAGAGCTTTCCCAACAGATTTTTTCCCGCCATTCTTCCCTCAATCCCACAACAAATTCTCAGTCTTCCATGGGAGGAGGAGGAGTTTTTCGGTCTCTGCTAAGTCGACCCAACACCCAGTACGCGTACGCTTCGCTCCTTCTCCCACCGGGAACCTCCATGTCGGTGGAGCTCGAACTGCGCTCTTCAATTATCTGTTTGCCAGGTCCAATGGAGGGAAGTTTGTTTTGAGAATTGAAGACACTGATTTGGAGAGGTCTACTAAGGAATCTGAGGAGGCTGTCTTACATGATCTCTCTTGGCTTGGCCTCAATTGGGATGAAGGACCTGGTGTGGATGGAGAGTATGGTCCATACCGACAATCAGAAAGAAATTTGTTGTACAAACAGTATGCTGAGAAGCTTGTAGAATCTGGTCATGTCTATCGCTGCTTTTGCTCTAGTGAGGAACTAGAGAAAATGAAGGAGATTGCAAAACTAAAACAACTGCCACCGGTATACACAGGGAAGTGGGCCAATGCAACAGACCAAGAAGTACAAGAAGAGCTGGAAAGAGGAACTCCATACACATATCGTTTTCGTGTGCCAAAGGAAGGGAGTTTAAAAATCAATGACCTTATTCGAGGCGAGGTCAGTTGGAACCTGAACACTCTTGGAGATTTTGTAATTATGAGAAGCAATGGTCAACCAGTGTACAATTTTTGCGTCACAGTTGATGATGCTACTATGGCCATCTCACATGTTATTAGAGCAGAGGAGCATTTACCAAATACTCTAAGGCAAGCATTAATATATAAGGCTCTAGGATTCCAAATGCCAGACTTTGCACATGTTTCCTTAATTCTTGCCCCTGATCGGAGTAAGCTGTCAAAGAGGCATGGTGCGACTTCTGTAGGCCAGTACAGGGAGATGGGCTATCTACCCCAGGCAATGGTGAACTACTTGGCACTGTTAGGTTGGGGTGATGGCACTGAGAATGAATTCTTCACCCTTGAGAAGCTAGTTGAGAAATTTACAATTGGTCGCGTCAACAAAAGTGGTGCCATTTTCGACTCGACCAAATTAAGGTGGATGAATGGTCAACACTTAAGAGCACTTCCATCTGAGGAGTTGACAAAGCTTATTGGCGAGCAGTGGAAAAATACTGGCCTCCTTACTGATTCAGAGGGCTCATTTGTTGAAGAGGCAGTAGAGCTGCTCAAAGATGGGATCGATTTAACACCAGATTCAGATAAAGCTCTCTCCAACTTGTTATCTTATCCTTTACATGACACTTTATCCAGTGCTGAAGCCAAACCAGTCTTAGAAGACAAACTTTCTGAGTTTGCTGCCAGTTTTATTGCTGCTTATGACAGCGGTGAGCTTGTTGGTGCACTTGAAGAAGGCCATGCTGGTTGGCAAAAGTGGGTTAAGAGCTTTGGCAAATCACTGAAGCGCAAGGGAAAATCACTGTTTATGCCTCTCCGAGTGCTGTTAACAGGAAAGTTCCATGGCCCAGATATGGGGACTAGCGTGCTTTTACTGCACAAAGCGGGAAAGTCTGGTATTGTTGCACCTCAAGCCGGGTTTGTGACAATAGATGAAAGGTTCAAAATATTGAGGGAAGTTGACTGGGAAGCATTCATCAAGGATCAGCCTCTCCTGGAGACTGCCACCATTGTATCAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006424 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006996 GO:0007005 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0035670 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0071704 GO:0071840 GO:0090304 GO:0090567 GO:0099402 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00860 ko00970 ko01100 ko01110 ko01120 map00860 map00970 map01100 map01110 map01120

Pfam Domains

Protein Families

tRNA-synt_1c

Protein Analysis

570

Amino Acids

63.92

Weight (kDa)

6.97

Isoelectric Point (pI)

36.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1c	PF00749	54 - 370	6.8e-104	tRNA synthetases class I (E and Q), catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015124)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64050
fragaria_vesca	FvH4_2g14910
malus_domestica	MD05G1011800.v1.1 MD10G1011800.v1.1
prunus_persica	Prupe.8G016800_v2.0.a1 Prupe.8G016800_v2.0.a1
pyrus_communis	pycom05g00680
rosa_chinensis	RchiOBHm_Chr6g0277491
rosa_laevigata	RLG00000013297
rosa_multiflora	Rmu_sc0017670.1_g000002
rosa_roxburghii	Rroxscaffold_7G00191270
rosa_rugosa	Rorug06G0108700
rosa_samantha	Rh6BG223400 Rh6CG225900 Rh6DG216200
rosa_wichuraiana	Rw6G019220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1079
AccB7I	CCANNNNNTGG	1 cut(s)	970
AccI	GTMKAC	3 cut(s)	146, 299, 537
AccII	CGCG	2 cut(s)	165, 1065
AccIII	TCCGGA	1 cut(s)	30
AciI	CCGC	3 cut(s)	68, 1395, 1571
AclWI	GGATC	3 cut(s)	22, 1256, 1677
AcoI	YGGCCR	1 cut(s)	768
AcsI	RAATTY	4 cut(s)	95, 412, 1019, 1049
AcuI	CTGAAG	2 cut(s)	1347, 1490
AfaI	GTAC	6 cut(s)	162, 168, 422, 576, 734, 941
AflII	CTTAAG	1 cut(s)	1126
AflIII	ACRYGT	3 cut(s)	163, 778, 868
AgeI	ACCGGT	1 cut(s)	532
AgsI	TTSAA	5 cut(s)	229, 278, 1220, 1418, 1630
AjnI	CCWGG	4 cut(s)	242, 367, 963, 1680
Alw21I	GWGCWC	3 cut(s)	214, 1135, 1414
Alw26I	GTCTC	2 cut(s)	139, 1679
Alw44I	GTGCAC	1 cut(s)	1410
AlwI	GGATC	3 cut(s)	22, 1256, 1677
Aor13HI	TCCGGA	1 cut(s)	30
AoxI	GGCC	7 cut(s)	346, 552, 768, 934, 1186, 1423, 1531
ApaLI	GTGCAC	1 cut(s)	1410
ApeKI	GCWGC	4 cut(s)	468, 1234, 1367, 1382
ApoI	RAATTY	4 cut(s)	95, 412, 1019, 1049
ArsI	GACNNNNNNTTYG	2 cut(s)	87, 119
AseI	ATTAAT	1 cut(s)	827
AsiGI	ACCGGT	1 cut(s)	532
Asp700I	GAANNNNTTC	1 cut(s)	1019
AspLEI	GCGC	2 cut(s)	223, 1476
AspS9I	GGNCC	5 cut(s)	246, 365, 389, 552, 1532
AsuC2I	CCSGG	2 cut(s)	190, 1603
AsuHPI	GGTGA	4 cut(s)	985, 1013, 1018, 1409
AvaII	GGWCC	3 cut(s)	246, 365, 389
BaeGI	GKGCMC	1 cut(s)	1414
BaeI	ACNNNNGTAYC	1 cut(s)	1683
BalI	TGGCCA	1 cut(s)	770
BanI	GGYRCC	1 cut(s)	1079
BanII	GRGCYC	2 cut(s)	214, 1211
BbsI	GAAGAC	3 cut(s)	97, 285, 1353
Bbv12I	GWGCWC	3 cut(s)	214, 1135, 1414
BbvI	GCAGC	4 cut(s)	455, 1221, 1354, 1369
BccI	CCATC	6 cut(s)	371, 779, 943, 998, 1147, 1238
BciT130I	CCWGG	4 cut(s)	244, 369, 965, 1682
BcnI	CCSGG	2 cut(s)	190, 1603
BcoDI	GTCTC	2 cut(s)	139, 1679
BfaI	CTAG	5 cut(s)	480, 491, 842, 1040, 1548
BfmI	CTRYAG	1 cut(s)	930
BfrI	CTTAAG	1 cut(s)	1126
BisI	GCNGC	4 cut(s)	469, 1235, 1368, 1383
BlsI	GCNGC	4 cut(s)	470, 1236, 1369, 1384
Bme1390I	CCNGG	6 cut(s)	190, 244, 369, 965, 1603, 1682
Bme18I	GGWCC	3 cut(s)	246, 365, 389
BmgT120I	GGNCC	5 cut(s)	246, 365, 389, 552, 1532
BmiI	GGNNCC	3 cut(s)	194, 680, 1081
BmrFI	CCNGG	6 cut(s)	190, 244, 369, 965, 1603, 1682
BmrI	ACTGGG	2 cut(s)	152, 1660
BmsI	GCATC	1 cut(s)	748
BmuI	ACTGGG	2 cut(s)	152, 1660
BpiI	GAAGAC	3 cut(s)	97, 285, 1353
BpmI	CTGGAG	1 cut(s)	1703
BpuEI	CTTGAG	2 cut(s)	1052, 1581
BpuMI	CCSGG	2 cut(s)	190, 1603
Bsa29I	ATCGAT	1 cut(s)	1251
BsaI	GGTCTC	1 cut(s)	139
BsaJI	CCNNGG	3 cut(s)	109, 963, 1528
BsaWI	WCCGGW	2 cut(s)	30, 532
BsaXI	ACNNNNNCTCC	4 cut(s)	109, 139, 687, 717
Bse118I	RCCGGY	1 cut(s)	532
Bse1I	ACTGG	8 cut(s)	158, 728, 937, 1189, 1320, 1337, 1371, 1655
Bse3DI	GCAATG	2 cut(s)	724, 975
BseAI	TCCGGA	1 cut(s)	30
BseBI	CCWGG	4 cut(s)	244, 369, 965, 1682
BseCI	ATCGAT	1 cut(s)	1251
BseDI	CCNNGG	3 cut(s)	109, 963, 1528
BseGI	GGATG	4 cut(s)	39, 364, 382, 1116
BseMI	GCAATG	2 cut(s)	724, 975
BseMII	CTCAG	6 cut(s)	114, 303, 426, 1002, 1134, 1350
BseNI	ACTGG	8 cut(s)	158, 728, 937, 1189, 1320, 1337, 1371, 1655
BseRI	GAGGAG	6 cut(s)	130, 133, 136, 329, 809, 1160
BseSI	GKGCMC	1 cut(s)	1414
BseXI	GCAGC	4 cut(s)	455, 1221, 1354, 1369
BsgI	GTGCAG	1 cut(s)	1547
Bsh1236I	CGCG	2 cut(s)	165, 1065
BshFI	GGCC	7 cut(s)	348, 554, 770, 936, 1188, 1425, 1533
BshNI	GGYRCC	1 cut(s)	1079
BshTI	ACCGGT	1 cut(s)	532
BshVI	ATCGAT	1 cut(s)	1251
BsiHKAI	GWGCWC	3 cut(s)	214, 1135, 1414
BsiSI	CCGG	4 cut(s)	31, 189, 533, 1602
BsiWI	CGTACG	1 cut(s)	166
BslFI	GGGAC	1 cut(s)	1558
BsmAI	GTCTC	2 cut(s)	139, 1679
BsmFI	GGGAC	1 cut(s)	1558
BsmI	GAATGC	1 cut(s)	1658
BsnI	GGCC	7 cut(s)	348, 554, 770, 936, 1188, 1425, 1533
Bso31I	GGTCTC	1 cut(s)	139
Bsp1286I	GDGCHC	4 cut(s)	214, 1135, 1211, 1414
Bsp13I	TCCGGA	1 cut(s)	30
Bsp1407I	TGTACA	2 cut(s)	420, 732
Bsp143I	GATC	5 cut(s)	27, 331, 892, 1248, 1669
Bsp19I	CCATGG	2 cut(s)	109, 1528
BspACI	CCGC	3 cut(s)	68, 1395, 1571
BspANI	GGCC	7 cut(s)	348, 554, 770, 936, 1188, 1425, 1533
BspCNI	CTCAG	6 cut(s)	113, 304, 427, 1003, 1135, 1351
BspDI	ATCGAT	1 cut(s)	1251
BspEI	TCCGGA	1 cut(s)	30
BspFNI	CGCG	2 cut(s)	165, 1065
BspLI	GGNNCC	3 cut(s)	194, 680, 1081
BspPI	GGATC	3 cut(s)	22, 1256, 1677
BspQI	GCTCTTC	2 cut(s)	230, 576
BspT107I	GGYRCC	1 cut(s)	1079
BspTI	CTTAAG	1 cut(s)	1126
BspTNI	GGTCTC	1 cut(s)	139
BsrDI	GCAATG	2 cut(s)	724, 975
BsrFI	RCCGGY	1 cut(s)	532
BsrGI	TGTACA	2 cut(s)	420, 732
BsrI	ACTGG	8 cut(s)	158, 728, 937, 1189, 1320, 1337, 1371, 1655
BssAI	RCCGGY	1 cut(s)	532
BssECI	CCNNGG	3 cut(s)	109, 963, 1528
BssMI	GATC	5 cut(s)	27, 331, 892, 1248, 1669
BssNAI	GTATAC	1 cut(s)	538
BssT1I	CCWWGG	2 cut(s)	109, 1528
Bst1107I	GTATAC	1 cut(s)	538
Bst2UI	CCWGG	4 cut(s)	244, 369, 965, 1682
Bst4CI	ACNGT	5 cut(s)	10, 429, 751, 990, 1491
Bst6I	CTCTTC	3 cut(s)	230, 576, 1215
BstAFI	CTTAAG	1 cut(s)	1126
BstAUI	TGTACA	2 cut(s)	420, 732
BstC8I	GCNNGC	3 cut(s)	822, 1169, 1553
BstDSI	CCRYGG	2 cut(s)	109, 1528
BstF5I	GGATG	4 cut(s)	39, 364, 382, 1116
BstFNI	CGCG	2 cut(s)	165, 1065
BstHHI	GCGC	2 cut(s)	223, 1476
BstKTI	GATC	5 cut(s)	30, 334, 895, 1251, 1672
BstMAI	GTCTC	2 cut(s)	139, 1679
BstMBI	GATC	5 cut(s)	27, 331, 892, 1248, 1669
BstMWI	GCNNNNNNNGC	5 cut(s)	218, 474, 767, 1561, 1598
BstNI	CCWGG	4 cut(s)	244, 369, 965, 1682
BstNSI	RCATGY	2 cut(s)	782, 872
BstSCI	CCNGG	6 cut(s)	188, 242, 367, 963, 1601, 1680
BstSFI	CTRYAG	1 cut(s)	930
BstSLI	GKGCMC	1 cut(s)	1414
BstUI	CGCG	2 cut(s)	165, 1065
BstV1I	GCAGC	4 cut(s)	455, 1221, 1354, 1369
BstV2I	GAAGAC	3 cut(s)	97, 285, 1353
BstX2I	RGATCY	1 cut(s)	27
BstXI	CCANNNNNNTGG	1 cut(s)	206
BstYI	RGATCY	1 cut(s)	27
BstZ17I	GTATAC	1 cut(s)	538
Bsu15I	ATCGAT	1 cut(s)	1251
BsuRI	GGCC	7 cut(s)	348, 554, 770, 936, 1188, 1425, 1533
BsuTUI	ATCGAT	1 cut(s)	1251
BtgI	CCRYGG	2 cut(s)	109, 1528
BtsCI	GGATG	4 cut(s)	39, 364, 382, 1116
BtsI	GCAGTG	1 cut(s)	1178
BtsIMutI	CAGTG	8 cut(s)	282, 735, 986, 1008, 1178, 1327, 1466, 1487
Cac8I	GCNNGC	3 cut(s)	822, 1169, 1553
CfoI	GCGC	2 cut(s)	223, 1476
Cfr10I	RCCGGY	1 cut(s)	532
Cfr13I	GGNCC	5 cut(s)	246, 365, 389, 552, 1532
ClaI	ATCGAT	1 cut(s)	1251
CseI	GACGC	2 cut(s)	733, 1054
CsiI	ACCWGGT	1 cut(s)	367
Csp6I	GTAC	6 cut(s)	161, 167, 421, 575, 733, 940
CspAI	ACCGGT	1 cut(s)	532
CspCI	CAANNNNNGTGG	2 cut(s)	1057, 1092
CviQI	GTAC	6 cut(s)	161, 167, 421, 575, 733, 940
DpnI	GATC	5 cut(s)	29, 333, 894, 1250, 1671
DpnII	GATC	5 cut(s)	27, 331, 892, 1248, 1669
DraI	TTTAAA	1 cut(s)	642
EaeI	YGGCCR	1 cut(s)	768
Eam1104I	CTCTTC	3 cut(s)	230, 576, 1215
EarI	CTCTTC	3 cut(s)	230, 576, 1215
Ecl136II	GAGCTC	1 cut(s)	212
Eco130I	CCWWGG	2 cut(s)	109, 1528
Eco24I	GRGCYC	2 cut(s)	214, 1211
Eco31I	GGTCTC	1 cut(s)	139
Eco47I	GGWCC	3 cut(s)	246, 365, 389
Eco53kI	GAGCTC	1 cut(s)	212
Eco57I	CTGAAG	2 cut(s)	1347, 1490
EcoICRI	GAGCTC	1 cut(s)	212
EcoO109I	RGGNCCY	1 cut(s)	365
EcoRI	GAATTC	1 cut(s)	1019
EcoRII	CCWGG	4 cut(s)	242, 367, 963, 1680
EcoT14I	CCWWGG	2 cut(s)	109, 1528
EcoT38I	GRGCYC	2 cut(s)	214, 1211
ErhI	CCWWGG	2 cut(s)	109, 1528
FalI	AAGNNNNNCTT	2 cut(s)	1338, 1370
FaqI	GGGAC	1 cut(s)	1558
FauI	CCCGC	2 cut(s)	75, 1564
FblI	GTMKAC	3 cut(s)	146, 299, 537
Fnu4HI	GCNGC	4 cut(s)	469, 1235, 1368, 1383
FokI	GGATG	4 cut(s)	46, 371, 389, 1123
FriOI	GRGCYC	2 cut(s)	214, 1211
Fsp4HI	GCNGC	4 cut(s)	469, 1235, 1368, 1383
FspBI	CTAG	5 cut(s)	480, 491, 842, 1040, 1548
GlaI	GCGC	2 cut(s)	222, 1475
GluI	GCNGC	4 cut(s)	469, 1235, 1368, 1383
GsuI	CTGGAG	1 cut(s)	1703
HaeIII	GGCC	7 cut(s)	348, 554, 770, 936, 1188, 1425, 1533
HapII	CCGG	4 cut(s)	31, 189, 533, 1602
HgaI	GACGC	2 cut(s)	733, 1054
HhaI	GCGC	2 cut(s)	223, 1476
Hin6I	GCGC	2 cut(s)	221, 1474
HinP1I	GCGC	2 cut(s)	221, 1474
HincII	GTYRAC	7 cut(s)	147, 725, 1069, 1121, 1152, 1515, 1648
HindII	GTYRAC	7 cut(s)	147, 725, 1069, 1121, 1152, 1515, 1648
HindIII	AAGCTT	2 cut(s)	440, 1157
HinfI	GANTC	6 cut(s)	308, 449, 846, 1091, 1199, 1265
HpaI	GTTAAC	1 cut(s)	1515
HpaII	CCGG	4 cut(s)	31, 189, 533, 1602
HphI	GGTGA	4 cut(s)	985, 1013, 1018, 1409
Hpy188I	TCNGA	8 cut(s)	313, 406, 897, 1144, 1204, 1270, 1360, 1505
Hpy188III	TCNNGA	1 cut(s)	31
HpyAV	CCTTC	5 cut(s)	189, 356, 496, 626, 1415
HpyCH4III	ACNGT	5 cut(s)	10, 429, 751, 990, 1491
HpyCH4V	TGCA	6 cut(s)	512, 560, 866, 1412, 1564, 1592
HpyF10VI	GCNNNNNNNGC	5 cut(s)	218, 474, 767, 1561, 1598
HspAI	GCGC	2 cut(s)	221, 1474
Kpn2I	TCCGGA	1 cut(s)	30
KspAI	GTTAAC	1 cut(s)	1515
Kzo9I	GATC	5 cut(s)	27, 331, 892, 1248, 1669
LguI	GCTCTTC	2 cut(s)	230, 576
LmnI	GCTCC	3 cut(s)	181, 209, 796
Lsp1109I	GCAGC	4 cut(s)	455, 1221, 1354, 1369
LweI	GCATC	1 cut(s)	748
MabI	ACCWGGT	1 cut(s)	367
MaeI	CTAG	5 cut(s)	480, 491, 842, 1040, 1548
MaeIII	GTNAC	2 cut(s)	745, 1609
MalI	GATC	5 cut(s)	29, 333, 894, 1250, 1671
MboI	GATC	5 cut(s)	27, 331, 892, 1248, 1669
MfeI	CAATTG	2 cut(s)	352, 1056
MflI	RGATCY	1 cut(s)	27
MhlI	GDGCHC	4 cut(s)	214, 1135, 1211, 1414
MlsI	TGGCCA	1 cut(s)	770
MluI	ACGCGT	1 cut(s)	163
MluNI	TGGCCA	1 cut(s)	770
MlyI	GAGTC	1 cut(s)	1085
MmeI	TCCRAC	2 cut(s)	656, 1308
Mox20I	TGGCCA	1 cut(s)	770
MroI	TCCGGA	1 cut(s)	30
MroXI	GAANNNNTTC	1 cut(s)	1019
MscI	TGGCCA	1 cut(s)	770
MseI	TTAA	8 cut(s)	641, 827, 878, 1103, 1127, 1256, 1449, 1514
MslI	CAYNNNNRTG	1 cut(s)	204
Msp20I	TGGCCA	1 cut(s)	770
MspA1I	CMGCKG	1 cut(s)	1395
MspCI	CTTAAG	1 cut(s)	1126
MspI	CCGG	4 cut(s)	31, 189, 533, 1602
MspR9I	CCNGG	6 cut(s)	190, 244, 369, 965, 1603, 1682
MunI	CAATTG	2 cut(s)	352, 1056
Mva1269I	GAATGC	1 cut(s)	1658
MvaI	CCWGG	4 cut(s)	244, 369, 965, 1682
MvnI	CGCG	2 cut(s)	165, 1065
MwoI	GCNNNNNNNGC	5 cut(s)	218, 474, 767, 1561, 1598
NciI	CCSGG	2 cut(s)	190, 1603
NcoI	CCATGG	2 cut(s)	109, 1528
NdeII	GATC	5 cut(s)	27, 331, 892, 1248, 1669
NlaIV	GGNNCC	3 cut(s)	194, 680, 1081
NmuCI	GTSAC	2 cut(s)	745, 1609
NspI	RCATGY	2 cut(s)	782, 872
PciI	ACATGT	2 cut(s)	778, 868
PciSI	GCTCTTC	2 cut(s)	230, 576
PctI	GAATGC	1 cut(s)	1658
PdmI	GAANNNNTTC	1 cut(s)	1019
PfeI	GAWTC	5 cut(s)	308, 449, 846, 1199, 1265
Pfl23II	CGTACG	1 cut(s)	166
PflMI	CCANNNNNTGG	1 cut(s)	970
PfoI	TCCNGGA	1 cut(s)	1680
PinAI	ACCGGT	1 cut(s)	532
PkrI	GCNGC	4 cut(s)	470, 1236, 1369, 1384
PleI	GAGTC	1 cut(s)	1085
PpsI	GAGTC	1 cut(s)	1085
PpuMI	RGGWCCY	1 cut(s)	365
PscI	ACATGT	2 cut(s)	778, 868
PshBI	ATTAAT	1 cut(s)	827
Psp124BI	GAGCTC	1 cut(s)	214
Psp5II	RGGWCCY	1 cut(s)	365
Psp6I	CCWGG	4 cut(s)	242, 367, 963, 1680
PspGI	CCWGG	4 cut(s)	242, 367, 963, 1680
PspLI	CGTACG	1 cut(s)	166
PspN4I	GGNNCC	3 cut(s)	194, 680, 1081
PspPI	GGNCC	5 cut(s)	246, 365, 389, 552, 1532
PspPPI	RGGWCCY	1 cut(s)	365
PsuI	RGATCY	1 cut(s)	27
RsaI	GTAC	6 cut(s)	162, 168, 422, 576, 734, 941
RsaNI	GTAC	6 cut(s)	161, 167, 421, 575, 733, 940
RseI	CAYNNNNRTG	1 cut(s)	204
SacI	GAGCTC	1 cut(s)	214
SalI	GTCGAC	1 cut(s)	145
SapI	GCTCTTC	2 cut(s)	230, 576
SaqAI	TTAA	8 cut(s)	641, 827, 878, 1103, 1127, 1256, 1449, 1514
SatI	GCNGC	4 cut(s)	469, 1235, 1368, 1383
Sau3AI	GATC	5 cut(s)	27, 331, 892, 1248, 1669
Sau96I	GGNCC	5 cut(s)	246, 365, 389, 552, 1532
SchI	GAGTC	1 cut(s)	1085
ScrFI	CCNGG	6 cut(s)	190, 244, 369, 965, 1603, 1682
SduI	GDGCHC	4 cut(s)	214, 1135, 1211, 1414
SexAI	ACCWGGT	1 cut(s)	367
SfaNI	GCATC	1 cut(s)	748
SfcI	CTRYAG	1 cut(s)	930
SinI	GGWCC	3 cut(s)	246, 365, 389
SmiMI	CAYNNNNRTG	1 cut(s)	204
SmlI	CTYRAG	3 cut(s)	1031, 1126, 1596
SmoI	CTYRAG	3 cut(s)	1031, 1126, 1596
SsiI	CCGC	3 cut(s)	68, 1395, 1571
SspI	AATATT	1 cut(s)	1635
SspMI	CTAG	5 cut(s)	480, 491, 842, 1040, 1548
SstI	GAGCTC	1 cut(s)	214
StyD4I	CCNGG	6 cut(s)	188, 242, 367, 963, 1601, 1680
StyI	CCWWGG	2 cut(s)	109, 1528
TaaI	ACNGT	5 cut(s)	10, 429, 751, 990, 1491
TaqI	TCGA	6 cut(s)	146, 214, 661, 1089, 1094, 1251
TaqII	GACCGA	1 cut(s)	121
TatI	WGTACW	4 cut(s)	420, 574, 732, 939
TfiI	GAWTC	5 cut(s)	308, 449, 846, 1199, 1265
Tru1I	TTAA	8 cut(s)	641, 827, 878, 1103, 1127, 1256, 1449, 1514
Tru9I	TTAA	8 cut(s)	641, 827, 878, 1103, 1127, 1256, 1449, 1514
TscAI	CASTG	8 cut(s)	289, 735, 993, 1015, 1178, 1327, 1473, 1494
TseFI	GTSAC	2 cut(s)	745, 1609
TseI	GCWGC	4 cut(s)	468, 1234, 1367, 1382
Tsp45I	GTSAC	2 cut(s)	745, 1609
TspDTI	ATGAA	7 cut(s)	50, 375, 515, 1032, 1127, 1635, 1651
TspRI	CASTG	8 cut(s)	289, 735, 993, 1015, 1178, 1327, 1473, 1494
Van91I	CCANNNNNTGG	1 cut(s)	970
Vha464I	CTTAAG	1 cut(s)	1126
VneI	GTGCAC	1 cut(s)	1410
VpaK11BI	GGWCC	3 cut(s)	246, 365, 389
VspI	ATTAAT	1 cut(s)	827
XapI	RAATTY	4 cut(s)	95, 412, 1019, 1049
XceI	RCATGY	2 cut(s)	782, 872
XmiI	GTMKAC	3 cut(s)	146, 299, 537
XmnI	GAANNNNTTC	1 cut(s)	1019
XspI	CTAG	5 cut(s)	480, 491, 842, 1040, 1548

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.