Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0019611

Physical Location & Seq

Genomic Coordinates Reverse (-)

7584970 .. 7602803

Gene Structure 17834 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1044300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGTGGAAGCTTCACCCCCAGCCTCTGCTGGCGATCAAGGCCAATGTCTCCGCTAACCCAAAAACCTGGATCGTTATCGGTCTCACCGTCGCAGGGATCGTGGTCCTGGCCGAGACCCACCGCCGAAGACGACGCCGAACCGGAACACAAATCCTTCTGTCCGAACCATTTATAGAGCCCCGCTTCCAACTCCTTCCCTTCCCTCAGCCCCCTCCCCCCGCCGCCACTCAACCCCTCGCCTCCCTCTCCTTCGCTGTCTCCGACATATTTGATGTGAAGGGTTATGTGACTGGGTTTGGGAGTCCCGATTGGGCGCGAACGCATGAAGCGGCGACCAAGACGGCGTTCGTGGTGACGGCTCTGCTAAAAAATGGGGCTACTTGCGTTGGCCACACCGTCATGGACGAGCTCAATTTTGGGATCACTGGTGAGAATGTTCATTACGGAACGCCTGTGAATCCTGAATTACAATCTCACATTCCGGGAGGATCTTCCAGTGGTTCTGCCGTGGCTGTTGCAGCCGGTCTTGTTGACTTTGCTCTTGGTATTGATACAACTGGATGTGTTAGAATTCCAGCATCATTTTGTGGTGTCATTGGGTACCGACCGTCTCATGGGGCTGTATCTATGCTTGGAGTTCTGCCAAATTCACAGAGTTTAGACGCTGTTGGAATTTTTGCCTGCAATCCATCTATTCTACGTCGTGTTGGACATGTTTTGCTTCAACTGAATTCTGTGGAACCTAAAAGGGCGAGATGCCTTATTTTTGCTGATGATCTCTTTCAGCTTCCTAAAATTCCTACTGAGAAGACAGTACATGTTGTCAGCAAAGCAATTGAACATCTATCTGGGTACCAACCTCCAAAGCATATGAATTTTAGTCAGTATATTGCTTCAAATGTACCCAGTTTAAAAGCATTTCGTGAAGATTCCACAAAACGTCAAAATGGAGTATTTACTTTGAAAGCTCTCTCTTCTGTGATGCTTTTGATACAGAGTTGTGAATTCAAAACCAATCATGAAGAATGGGTTAAATCAGCGAAACCAAAGCTAGGGCCAGATGTATCAGATCGTGTTCGTGCAGCAATTAACTCTACAAATGAAAACATGAAAACCCTATATAAAGTAAGGACTGAGATGAGAGCTGCCCTTCAAAGTCTTTTAAAGGATGATGGAATATTAGTGATTCCCACAGTCGCAGATCCTCCATTAAAGCTTAATACAAAGAAAGGGGGGTCATCTGAGTTTAATGATAGAACATTAGCATTAACAAGCATTGTCAGCATGTCTGGGTGCTGTCAGGTTACAGTACCAGTAGGAAAGCACAATGACTGCCCTATCTCTGTTTCATTCATCGCGTTCCATGGTGTTGATAAATTTCTCCTTGATACAGTCTTGGATATGTACTCTTCAGTTCAGGAGAAAGTAAGCATTACATCCCATTCATTGCCATTGCCAGATACCAATGGGAACATGGATGCTTCAGAACTCTTAAAAGAGAAGGGAAATGCTGCATTTAAGGGAAGGCAATGGAATAAGGCTGTCAACTATTATACTGAAGCTATCAAATTGAACATGATGAGCGCAACTTACTACTCCAACCGCGCAGCTGCTTACTTGGAGTTGGGGTGCTTCCAGCAAGCTGAAGAGGACTGCAGCAAGGCAATATCACTTGACAAAAAGAATGTGAAGGCATATCTTAGACGCGGAACAGCTAGAGAATCACTTCTTTGTTATAAGGAGGCTGCTCAAGATTTCAAGCATGCCCTTGTTCTTGAACCTCAAAATAAGGTTGCCAGTCTTGCTGAAAAAAGACTAAAGAAACTGCTGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0009987 GO:0015031 GO:0015833 GO:0016043 GO:0033036 GO:0033365 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0072655

KEGG Pathways

Metabolic & Signaling

ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120

Pfam Domains

Protein Families

Amidase TPR_1 TPR_11

Protein Analysis

612

Amino Acids

66.67

Weight (kDa)

9.2

Isoelectric Point (pI)

36.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	76 - 462	1.5e-54	Amidase
TPR_BSK1_C	PF25575	491 - 561	7.5e-08	Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_11	PF13414	502 - 543	7e-06	TPR repeat
TPR_1	PF00515	530 - 562	2.5e-07	Tetratricopeptide repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019611)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09420
malus_domestica	MD05G1044300.v1.1 MD10G1051400.v1.1
prunus_persica	Prupe.8G059900_v2.0.a1 Prupe.8G059900_v2.0.a1
pyrus_communis	pycom05g03700 pycom10g03620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1737
Acc65I	GGTACC	2 cut(s)	600, 852
AccB1I	GGYRCC	2 cut(s)	600, 852
AccII	CGCG	4 cut(s)	316, 1358, 1605, 1707
AciI	CCGC	8 cut(s)	51, 121, 181, 219, 222, 329, 1603, 1707
AclWI	GGATC	5 cut(s)	77, 104, 428, 496, 1196
AcoI	YGGCCR	2 cut(s)	108, 388
AcsI	RAATTY	8 cut(s)	570, 646, 672, 730, 795, 874, 1004, 1376
AcuI	CTGAAG	4 cut(s)	1395, 1467, 1578, 1665
AcyI	GRCGYC	1 cut(s)	133
AfaI	GTAC	6 cut(s)	602, 816, 854, 903, 1311, 1406
AfiI	CCNNNNNNNGG	3 cut(s)	93, 614, 1052
AflIII	ACRYGT	2 cut(s)	712, 817
AgsI	TTSAA	9 cut(s)	725, 839, 897, 964, 1009, 1154, 1573, 1759, 1778
AjnI	CCWGG	2 cut(s)	65, 105
AjuI	GAANNNNNNNTTGG	2 cut(s)	329, 361
Alw21I	GWGCWC	1 cut(s)	411
Alw26I	GTCTC	5 cut(s)	52, 86, 107, 262, 615
AlwI	GGATC	5 cut(s)	77, 104, 428, 496, 1196
AlwNI	CAGNNNCTG	1 cut(s)	25
AoxI	GGCC	4 cut(s)	39, 108, 388, 1055
ApeKI	GCWGC	8 cut(s)	518, 1082, 1145, 1511, 1607, 1610, 1656, 1745
ApoI	RAATTY	8 cut(s)	570, 646, 672, 730, 795, 874, 1004, 1376
Asp700I	GAANNNNTTC	1 cut(s)	1725
Asp718I	GGTACC	2 cut(s)	600, 852
AspLEI	GCGC	3 cut(s)	316, 1586, 1607
AspS9I	GGNCC	2 cut(s)	103, 1055
AsuC2I	CCSGG	1 cut(s)	483
AsuHPI	GGTGA	4 cut(s)	5, 76, 364, 440
AvaII	GGWCC	1 cut(s)	103
BalI	TGGCCA	1 cut(s)	390
BanI	GGYRCC	2 cut(s)	600, 852
BanII	GRGCYC	2 cut(s)	180, 411
BbsI	GAAGAC	2 cut(s)	133, 815
Bbv12I	GWGCWC	1 cut(s)	411
BbvCI	CCTCAGC	1 cut(s)	204
BbvI	GCAGC	8 cut(s)	530, 1094, 1132, 1498, 1597, 1619, 1668, 1732
BccI	CCATC	2 cut(s)	697, 1166
BceAI	ACGGC	3 cut(s)	357, 372, 491
BciT130I	CCWGG	2 cut(s)	67, 107
BcnI	CCSGG	1 cut(s)	483
BcoDI	GTCTC	5 cut(s)	52, 86, 107, 262, 615
BfaI	CTAG	2 cut(s)	1052, 1716
BfmI	CTRYAG	1 cut(s)	1654
Bme1390I	CCNGG	3 cut(s)	67, 107, 483
Bme18I	GGWCC	1 cut(s)	103
BmgT120I	GGNCC	2 cut(s)	103, 1055
BmiI	GGNNCC	3 cut(s)	602, 741, 854
BmrFI	CCNGG	3 cut(s)	67, 107, 483
BmrI	ACTGGG	2 cut(s)	300, 900
BmsI	GCATC	4 cut(s)	587, 746, 972, 1468
BmuI	ACTGGG	2 cut(s)	300, 900
BpiI	GAAGAC	2 cut(s)	133, 815
Bpu10I	CCTNAGC	1 cut(s)	204
BpuEI	CTTGAG	1 cut(s)	1734
BpuMI	CCSGG	1 cut(s)	483
BsaBI	GATNNNNATC	1 cut(s)	74
BsaHI	GRCGYC	1 cut(s)	133
BsaI	GGTCTC	2 cut(s)	86, 107
BsaJI	CCNNGG	2 cut(s)	507, 1363
BsaWI	WCCGGW	1 cut(s)	140
BsaXI	ACNNNNNCTCC	2 cut(s)	1613, 1643
Bsc4I	CCNNNNNNNGG	3 cut(s)	93, 614, 1052
Bse118I	RCCGGY	1 cut(s)	521
Bse1I	ACTGG	7 cut(s)	295, 430, 495, 562, 906, 1313, 1797
Bse3DI	GCAATG	3 cut(s)	1445, 1451, 1535
Bse8I	GATNNNNATC	1 cut(s)	74
BseBI	CCWGG	2 cut(s)	67, 107
BseDI	CCNNGG	2 cut(s)	507, 1363
BseGI	GGATG	4 cut(s)	566, 1174, 1436, 1483
BseJI	GATNNNNATC	1 cut(s)	74
BseLI	CCNNNNNNNGG	3 cut(s)	93, 614, 1052
BseMI	GCAATG	3 cut(s)	1445, 1451, 1535
BseMII	CTCAG	5 cut(s)	218, 795, 1125, 1233, 1820
BseNI	ACTGG	7 cut(s)	295, 430, 495, 562, 906, 1313, 1797
BseXI	GCAGC	8 cut(s)	530, 1094, 1132, 1498, 1597, 1619, 1668, 1732
BseYI	CCCAGC	1 cut(s)	18
BsgI	GTGCAG	1 cut(s)	1101
Bsh1236I	CGCG	4 cut(s)	316, 1358, 1605, 1707
Bsh1285I	CGRYCG	1 cut(s)	608
BshFI	GGCC	4 cut(s)	41, 110, 390, 1057
BshNI	GGYRCC	2 cut(s)	600, 852
BsiEI	CGRYCG	1 cut(s)	608
BsiHKAI	GWGCWC	1 cut(s)	411
BsiSI	CCGG	3 cut(s)	141, 482, 522
BslFI	GGGAC	1 cut(s)	288
BslI	CCNNNNNNNGG	3 cut(s)	93, 614, 1052
BsmAI	GTCTC	5 cut(s)	52, 86, 107, 262, 615
BsmBI	CGTCTC	1 cut(s)	615
BsmFI	GGGAC	1 cut(s)	288
BsnI	GGCC	4 cut(s)	41, 110, 390, 1057
Bso31I	GGTCTC	2 cut(s)	86, 107
Bsp1286I	GDGCHC	2 cut(s)	180, 411
Bsp143I	GATC	8 cut(s)	33, 69, 96, 420, 488, 775, 1069, 1201
Bsp19I	CCATGG	1 cut(s)	1363
BspACI	CCGC	8 cut(s)	51, 121, 181, 219, 222, 329, 1603, 1707
BspANI	GGCC	4 cut(s)	41, 110, 390, 1057
BspCNI	CTCAG	5 cut(s)	217, 796, 1126, 1234, 1821
BspFNI	CGCG	4 cut(s)	316, 1358, 1605, 1707
BspHI	TCATGA	1 cut(s)	1018
BspLI	GGNNCC	3 cut(s)	602, 741, 854
BspMAI	CTGCAG	1 cut(s)	1658
BspPI	GGATC	5 cut(s)	77, 104, 428, 496, 1196
BspT107I	GGYRCC	2 cut(s)	600, 852
BspTNI	GGTCTC	2 cut(s)	86, 107
BsrDI	GCAATG	3 cut(s)	1445, 1451, 1535
BsrFI	RCCGGY	1 cut(s)	521
BsrI	ACTGG	7 cut(s)	295, 430, 495, 562, 906, 1313, 1797
BssAI	RCCGGY	1 cut(s)	521
BssECI	CCNNGG	2 cut(s)	507, 1363
BssMI	GATC	8 cut(s)	33, 69, 96, 420, 488, 775, 1069, 1201
BssNI	GRCGYC	1 cut(s)	133
BssT1I	CCWWGG	1 cut(s)	1363
Bst2UI	CCWGG	2 cut(s)	67, 107
Bst4CI	ACNGT	7 cut(s)	88, 397, 609, 814, 1195, 1309, 1393
Bst6I	CTCTTC	3 cut(s)	979, 1414, 1641
BstACI	GRCGYC	1 cut(s)	133
BstC8I	GCNNGC	4 cut(s)	30, 682, 1641, 1764
BstDEI	CTNAG	6 cut(s)	204, 804, 1134, 1242, 1700, 1829
BstDSI	CCRYGG	2 cut(s)	507, 1363
BstF5I	GGATG	4 cut(s)	566, 1174, 1436, 1483
BstFNI	CGCG	4 cut(s)	316, 1358, 1605, 1707
BstHHI	GCGC	3 cut(s)	316, 1586, 1607
BstKTI	GATC	8 cut(s)	36, 72, 99, 423, 491, 778, 1072, 1204
BstMAI	GTCTC	5 cut(s)	52, 86, 107, 262, 615
BstMBI	GATC	8 cut(s)	33, 69, 96, 420, 488, 775, 1069, 1201
BstMCI	CGRYCG	1 cut(s)	608
BstMWI	GCNNNNNNNGC	4 cut(s)	38, 518, 1281, 1802
BstNI	CCWGG	2 cut(s)	67, 107
BstNSI	RCATGY	4 cut(s)	716, 821, 1288, 1766
BstSCI	CCNGG	3 cut(s)	65, 105, 481
BstSFI	CTRYAG	1 cut(s)	1654
BstUI	CGCG	4 cut(s)	316, 1358, 1605, 1707
BstV1I	GCAGC	8 cut(s)	530, 1094, 1132, 1498, 1597, 1619, 1668, 1732
BstV2I	GAAGAC	2 cut(s)	133, 815
BstX2I	RGATCY	2 cut(s)	488, 1201
BstXI	CCANNNNNNTGG	1 cut(s)	66
BstYI	RGATCY	2 cut(s)	488, 1201
BsuRI	GGCC	4 cut(s)	41, 110, 390, 1057
BtgI	CCRYGG	2 cut(s)	507, 1363
BtgZI	GCGATG	1 cut(s)	1339
BtsCI	GGATG	4 cut(s)	566, 1174, 1436, 1483
BtsIMutI	CAGTG	2 cut(s)	423, 502
Cac8I	GCNNGC	4 cut(s)	30, 682, 1641, 1764
CaiI	CAGNNNCTG	1 cut(s)	25
CciI	TCATGA	1 cut(s)	1018
CfoI	GCGC	3 cut(s)	316, 1586, 1607
Cfr10I	RCCGGY	1 cut(s)	521
Cfr13I	GGNCC	2 cut(s)	103, 1055
CseI	GACGC	3 cut(s)	141, 671, 1713
Csp6I	GTAC	6 cut(s)	601, 815, 853, 902, 1310, 1405
CviQI	GTAC	6 cut(s)	601, 815, 853, 902, 1310, 1405
DdeI	CTNAG	6 cut(s)	204, 804, 1134, 1242, 1700, 1829
DpnI	GATC	8 cut(s)	35, 71, 98, 422, 490, 777, 1071, 1203
DpnII	GATC	8 cut(s)	33, 69, 96, 420, 488, 775, 1069, 1201
DraI	TTTAAA	2 cut(s)	912, 1164
EaeI	YGGCCR	2 cut(s)	108, 388
Eam1104I	CTCTTC	3 cut(s)	979, 1414, 1641
EarI	CTCTTC	3 cut(s)	979, 1414, 1641
Ecl136II	GAGCTC	1 cut(s)	409
Eco130I	CCWWGG	1 cut(s)	1363
Eco24I	GRGCYC	2 cut(s)	180, 411
Eco31I	GGTCTC	2 cut(s)	86, 107
Eco47I	GGWCC	1 cut(s)	103
Eco53kI	GAGCTC	1 cut(s)	409
Eco57I	CTGAAG	4 cut(s)	1395, 1467, 1578, 1665
EcoICRI	GAGCTC	1 cut(s)	409
EcoRI	GAATTC	3 cut(s)	570, 730, 1004
EcoRII	CCWGG	2 cut(s)	65, 105
EcoT14I	CCWWGG	1 cut(s)	1363
EcoT38I	GRGCYC	2 cut(s)	180, 411
ErhI	CCWWGG	1 cut(s)	1363
Esp3I	CGTCTC	1 cut(s)	615
FaqI	GGGAC	1 cut(s)	288
FauI	CCCGC	2 cut(s)	188, 226
FauNDI	CATATG	1 cut(s)	870
FokI	GGATG	4 cut(s)	573, 1181, 1423, 1490
FriOI	GRGCYC	2 cut(s)	180, 411
FspBI	CTAG	2 cut(s)	1052, 1716
GlaI	GCGC	3 cut(s)	315, 1585, 1606
GsaI	CCCAGC	1 cut(s)	22
HaeIII	GGCC	4 cut(s)	41, 110, 390, 1057
HapII	CCGG	3 cut(s)	141, 482, 522
HgaI	GACGC	3 cut(s)	141, 671, 1713
HhaI	GCGC	3 cut(s)	316, 1586, 1607
Hin1I	GRCGYC	1 cut(s)	133
Hin6I	GCGC	3 cut(s)	314, 1584, 1605
HinP1I	GCGC	3 cut(s)	314, 1584, 1605
HincII	GTYRAC	2 cut(s)	532, 1546
HindII	GTYRAC	2 cut(s)	532, 1546
HindIII	AAGCTT	2 cut(s)	8, 1214
HinfI	GANTC	5 cut(s)	301, 457, 929, 1186, 1721
HpaII	CCGG	3 cut(s)	141, 482, 522
HphI	GGTGA	4 cut(s)	5, 76, 364, 440
Hpy166II	GTNNAC	2 cut(s)	532, 1546
Hpy188I	TCNGA	5 cut(s)	163, 262, 1069, 1243, 1486
Hpy188III	TCNNGA	7 cut(s)	305, 461, 923, 1019, 1418, 1751, 1775
Hpy8I	GTNNAC	2 cut(s)	532, 1546
Hpy99I	CGWCG	3 cut(s)	92, 135, 705
HpyAV	CCTTC	9 cut(s)	164, 203, 208, 259, 271, 1160, 1495, 1518, 1684
HpyCH4III	ACNGT	7 cut(s)	88, 397, 609, 814, 1195, 1309, 1393
HpyCH4IV	ACGT	2 cut(s)	700, 940
HpyCH4V	TGCA	5 cut(s)	518, 684, 1082, 1514, 1656
HpyF10VI	GCNNNNNNNGC	4 cut(s)	38, 518, 1281, 1802
HpyF3I	CTNAG	6 cut(s)	204, 804, 1134, 1242, 1700, 1829
HpySE526I	ACGT	2 cut(s)	700, 940
Hsp92I	GRCGYC	1 cut(s)	133
HspAI	GCGC	3 cut(s)	314, 1584, 1605
KpnI	GGTACC	2 cut(s)	604, 856
Kzo9I	GATC	8 cut(s)	33, 69, 96, 420, 488, 775, 1069, 1201
Lsp1109I	GCAGC	8 cut(s)	530, 1094, 1132, 1498, 1597, 1619, 1668, 1732
LweI	GCATC	4 cut(s)	587, 746, 972, 1468
MaeI	CTAG	2 cut(s)	1052, 1716
MaeII	ACGT	2 cut(s)	700, 940
MaeIII	GTNAC	3 cut(s)	286, 352, 1303
MalI	GATC	8 cut(s)	35, 71, 98, 422, 490, 777, 1071, 1203
MboI	GATC	8 cut(s)	33, 69, 96, 420, 488, 775, 1069, 1201
MboII	GAAGA	8 cut(s)	138, 483, 820, 938, 966, 1034, 1401, 1658
MfeI	CAATTG	1 cut(s)	834
MflI	RGATCY	2 cut(s)	488, 1201
MhlI	GDGCHC	2 cut(s)	180, 411
MlsI	TGGCCA	1 cut(s)	390
MluNI	TGGCCA	1 cut(s)	390
MlyI	GAGTC	1 cut(s)	310
MmeI	TCCRAC	5 cut(s)	211, 285, 649, 688, 1623
Mox20I	TGGCCA	1 cut(s)	390
MroXI	GAANNNNTTC	1 cut(s)	1725
MscI	TGGCCA	1 cut(s)	390
MslI	CAYNNNNRTG	1 cut(s)	398
Msp20I	TGGCCA	1 cut(s)	390
MspA1I	CMGCKG	1 cut(s)	1610
MspI	CCGG	3 cut(s)	141, 482, 522
MspR9I	CCNGG	3 cut(s)	67, 107, 483
MunI	CAATTG	1 cut(s)	834
MvaI	CCWGG	2 cut(s)	67, 107
MvnI	CGCG	4 cut(s)	316, 1358, 1605, 1707
MwoI	GCNNNNNNNGC	4 cut(s)	38, 518, 1281, 1802
NciI	CCSGG	1 cut(s)	483
NcoI	CCATGG	1 cut(s)	1363
NdeI	CATATG	1 cut(s)	870
NdeII	GATC	8 cut(s)	33, 69, 96, 420, 488, 775, 1069, 1201
NlaIV	GGNNCC	3 cut(s)	602, 741, 854
NmeAIII	GCCGAG	1 cut(s)	136
NmuCI	GTSAC	2 cut(s)	286, 352
NspI	RCATGY	4 cut(s)	716, 821, 1288, 1766
PaeI	GCATGC	1 cut(s)	1766
PagI	TCATGA	1 cut(s)	1018
PciI	ACATGT	2 cut(s)	712, 817
PcsI	WCGNNNNNNNCGW	3 cut(s)	84, 96, 258
PdmI	GAANNNNTTC	1 cut(s)	1725
PfeI	GAWTC	4 cut(s)	457, 929, 1186, 1721
PfoI	TCCNGGA	1 cut(s)	481
PleI	GAGTC	1 cut(s)	309
PpsI	GAGTC	1 cut(s)	309
PscI	ACATGT	2 cut(s)	712, 817
PsiI	TTATAA	1 cut(s)	1737
Psp124BI	GAGCTC	1 cut(s)	411
Psp6I	CCWGG	2 cut(s)	65, 105
PspFI	CCCAGC	1 cut(s)	18
PspGI	CCWGG	2 cut(s)	65, 105
PspN4I	GGNNCC	3 cut(s)	602, 741, 854
PspPI	GGNCC	2 cut(s)	103, 1055
PstI	CTGCAG	1 cut(s)	1658
PstNI	CAGNNNCTG	1 cut(s)	25
PsuI	RGATCY	2 cut(s)	488, 1201
PvuII	CAGCTG	1 cut(s)	1610
RsaI	GTAC	6 cut(s)	602, 816, 854, 903, 1311, 1406
RsaNI	GTAC	6 cut(s)	601, 815, 853, 902, 1310, 1405
RseI	CAYNNNNRTG	1 cut(s)	398
SacI	GAGCTC	1 cut(s)	411
Sau3AI	GATC	8 cut(s)	33, 69, 96, 420, 488, 775, 1069, 1201
Sau96I	GGNCC	2 cut(s)	103, 1055
SchI	GAGTC	1 cut(s)	310
ScrFI	CCNGG	3 cut(s)	67, 107, 483
SduI	GDGCHC	2 cut(s)	180, 411
SfaNI	GCATC	4 cut(s)	587, 746, 972, 1468
SfcI	CTRYAG	1 cut(s)	1654
SinI	GGWCC	1 cut(s)	103
SmiMI	CAYNNNNRTG	1 cut(s)	398
SmlI	CTYRAG	1 cut(s)	1749
SmoI	CTYRAG	1 cut(s)	1749
SphI	GCATGC	1 cut(s)	1766
SsiI	CCGC	8 cut(s)	51, 121, 181, 219, 222, 329, 1603, 1707
SspI	AATATT	1 cut(s)	1179
SspMI	CTAG	2 cut(s)	1052, 1716
SstI	GAGCTC	1 cut(s)	411
StyD4I	CCNGG	3 cut(s)	65, 105, 481
StyI	CCWWGG	1 cut(s)	1363
TaaI	ACNGT	7 cut(s)	88, 397, 609, 814, 1195, 1309, 1393
TaiI	ACGT	2 cut(s)	703, 943
TaqII	GACCGA	1 cut(s)	68
TatI	WGTACW	2 cut(s)	814, 1404
TauI	GCSGC	2 cut(s)	224, 332
TfiI	GAWTC	4 cut(s)	457, 929, 1186, 1721
TscAI	CASTG	2 cut(s)	430, 502
TseFI	GTSAC	2 cut(s)	286, 352
TseI	GCWGC	8 cut(s)	518, 1082, 1145, 1511, 1607, 1610, 1656, 1745
Tsp45I	GTSAC	2 cut(s)	286, 352
TspDTI	ATGAA	9 cut(s)	339, 428, 887, 1035, 1116, 1124, 1338, 1342, 1434
TspGWI	ACGGA	1 cut(s)	459
TspRI	CASTG	2 cut(s)	430, 502
VpaK11BI	GGWCC	1 cut(s)	103
XapI	RAATTY	8 cut(s)	570, 646, 672, 730, 795, 874, 1004, 1376
XceI	RCATGY	4 cut(s)	716, 821, 1288, 1766
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1471
XmnI	GAANNNNTTC	1 cut(s)	1725
XspI	CTAG	2 cut(s)	1052, 1716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.